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The metropolis of Kolkata stands uniquely positioned to implement a natural sewage treatment paradigm through the utilization of waste stabilization ponds, specifically within the East Kolkata Wetlands (EKW). These shallow oxidation ponds harness solar irradiation and algae bacteria symbiotic processes to effectively treat incoming sewage. Concurrently, nutrient-rich effluents are assimilated through fish production, converting available nutrients into protein—a hallmark of nature-based treatment. A portion of raw sewage is used to cultivate a chunk of vegetables before treatment in fish ponds, and the reclaimed water after treatment is used for vegetable and paddy cultivation downstream. This investigation explains the delineation of a sewage flow system to EKW, a Ramsar-designated site. Substantively, it offers quantitative insights into the sewage volumes and quality undergoing treatment. The sewage flow is higher in the winter months (909.07 MLD) compared to the summer months (709.34 MLD). In general, the sewage from the Kolkata city flowing to the EKW is moderately polluted. Extensive scrutiny of sewage from pond inlets and outlets serves as a quantitative metric for evaluating treatment efficacy. EKW efficiently treats the sewage, demonstrating 59.1% Biological Oxygen Demand (BOD) removal and a 99.28% reduction in fecal coliform. The natural treatment system excels in removing ammoniacal nitrogen (80.38%) and phosphate (90%). The treated water’s quality along the EKW boundary, culminating at the Kulti Gong River discharge point, was systematically assessed. Analytical findings indicate that all measured concentrations in the treated water adhere to prescribed inland surface water discharge standards prescribed by the Central Pollution Control Board, India, barring a marginal elevation in BOD during winter. Evidently, the EKW system adeptly manages substantial sewage volumes, fostering efficient treatment while concurrently facilitating resource recovery through fish production, yielding economic dividends. Despite its substantial land footprint, preserving this inherently sustainable wastewater management paradigm is imperative.

Background: Contracted subscapularis tears, especially Lafosse grade 3 and 4, pose a significant challenge in surgical repair due to the need for extensive release of retracted tendon and the risk of cut-through due to poor tissue quality. The posterior viewing portal may hinder adequate visualization and extensive release of retracted tendons, particularly in addressing the bursal surface of the tears. Tension-free robust repair is critical to prevent retears and for achieving successful outcomes for these massive and complex retracted tears. This study investigates the clinical outcomes of the Lasso-Loop technique with an anterolateral viewing portal, aiming to enhance tendon release, improve footprint fixation, and optimize tissue grip, particularly in cases of chronic retracted Lafosse 3 and 4 tears. This innovation also eliminates the need for arthroscope switching and serves as a crucial measure for averting axillary nerve injuries during the procedure. Methods: The study included 40 patients with isolated subscapularis or combined anterosuperior rotator cuff tears (Lafosse grade 3 and 4) that underwent arthroscopic subscapularis repair using a Lasso-Loop technique through an anterolateral viewing portal. Evaluations were conducted both preoperatively and at predefined postoperative intervals: 6 weeks, 3 months, 6 months, 1 year, and 2 years. The evaluations in this study encompassed a comprehensive range of clinical assessments, which included a complete range of movements, specialized tests for subscapularis muscle functionality (specifically the lift-off test and belly press test), the utilization of the University of California at Los Angeles and Oxford shoulder scoring systems, and a postoperative ultrasonography conducted at the 6-month mark to assess the presence of any retear. Clinical outcomes were compared with that of the opposite normal shoulder. Results: There was considerable improvement in the active painless range of motion postoperatively. During the 2-year follow-up, the positive belly press test decreased from 50% (n = 20) to 2.5% (n = 1), while the positive lift-off test decreased from 100% (n = 40) to 5% (n = 2). The modified University of California at Los Angeles score increased significantly from 10.15 preoperatively to 30.17 at 2 years postoperatively (P value = .001). Oxford’s Shoulder Score increased significantly from 15.6 preoperatively to 40.64 at 2 years postoperatively (P value = .001). Conclusion: This technique has produced positive functional outcomes, in cases categorized as massive subscapularis tears and chronic tears indicating the superiority over other conventional techniques of subscapularis repair described in the literature.

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Impacts of hydrogen annealing on crystallographic characterization, electronic structure, and optical, photocatalytic, and magnetic properties of polycrystalline Zn1−xCoxO (x = 0.01–0.06) samples have been considered. Structural analyses based on powder X-ray diffraction, Rietveld refinement, and Raman spectroscopy prove all materials having the P63mc wurtzite-type structure. The Co-doping and hydrogenation changed the concentration of Zn– and O–related defects whose energy levels occupy the band gap. This also enhanced photocatalytic performance of hydrogenated samples with x > 0.02. X-ray and UV–Vis absorption analyses indicate the substitution of Co2+ for Zn2+ in the wurtzite-type ZnO lattice, leading to irregularly changed the unit-cell parameters. While all the as-prepared samples are paramagnetic, the hydrogenated ones exhibit weak ferromagnetism. Ferromagnetic (FM) ordering increases when x increases, particularly for x ≥ 0.02. According to the results achieved from studying crystalline and electronic structures, we believe that oxygen-vacancies-mediated interactions between Co2+ ions and H–Co–H exchange dimers enhanced FM ordering in hydrogenated Zn1−xCoxO. Computational investigations have also indicated that the magnetization value of H–Zn1−xCoxO is influenced by the positioning of the H dopant, meaning that the couplings between Co and H play an essential role in establishing FM order in H–Zn1−xCoxO. [ABSTRACT FROM AUTHOR]

Khaista Rahman,1,* Shahid Ullah Khan,2,* Shah Fahad,2,3 Ming Xian Chang,4,5 Aqleem Abbas,6 Wasim Ullah Khan,7 Lutfur Rahman,8 Zaheer Ul Haq,9 Ghulam Nabi,4,5 Dilfaraz Khan10 1College of Animal Sciences/State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, China; 2College of Plant Sciences and Technology/National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; 3Department of Agriculture, The University of Swabi, Khyber Pakhtunkhwa, Anbar, Pakistan; 4State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, the Chinese Academy of Sciences, Wuhan 430072, Hubei, China; 5University of the Chinese Academy of Sciences, Shijingshan District, Beijing, China; 6Provincial Key Lab of Plant Pathology of Hubei Province, Huazhong Agricultural University, Hongshan District, Wuhan 430070, China; 7School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou, China; 8Molecular Systematics & Applied Ethno Botany Lab (MoSEL), Department of Biotechnology, Quaid I Azam University, Islamabad, Pakistan; 9School of Chemistry and Chemical Engineering, Shanghai Jiao tong University, Shanghai, China; 10Institute of Chemical Sciences, Gomal University, Dera Ismail Khan, Pakistan *These authors contributed equally to this work Abstract: Malaria, the exterminator of ~1.5 to 2.7 million human lives yearly, is a notorious disease known throughout the world. The eradication of this disease is difficult and a challenge to scientists. Vector elimination and effective chemotherapy for the patients are key tactics to be used in the fight against malaria. However, drug resistance and environmental and social concerns are the main hurdles in this fight against malaria. Overcoming these limitations is the major challenge for the 21st-century malarial researchers. Adapting the principles of nano-biotechnology to both vector control and patient therapy is the only solution to the problem. Several compounds such as lipids, proteins, nucleic acid and metallic nanoparticles (NPs) have been successfully used for the control of this lethal malaria disease. Other useful natural reagents such as microbes and their products, carbohydrates, vitamins, plant extracts and biodegradable polymers, are also used to control this disease. Among these particles, the plant-based particles such as leaf, root, stem, latex, and seed give the best antagonistic response against malaria. In the present review, we describe certain efforts related to the control, prevention and treatment of malaria. We hope that this review will open new doors for malarial research. Keywords: malaria, vectors, chemotherapy, drug resistance, nano-biotechnology

Shahid Ullah Khan,1,2 Tawfik A Saleh,3 Abdul Wahab,4 Muhammad Hafeez Ullah Khan,1,2 Dilfaraz Khan,5 Wasim Ullah Khan,6 Abdur Rahim,7 Sajid Kamal,8 Farman Ullah Khan,9 Shah Fahad1,10 1College of Plant Sciences and Technology, 2National Key Laboratory of Crop Genetics Improvement, Huazhong Agricultural University, Wuhan, People’s Republic of China; 3Department of Chemistry, King Fahd University of Petroleum & Minerals, Dhahran, Saudi Arabia; 4Department of Pharmacy, Kohat University of Science and Technology, Kohat, 5Institute of Chemical Sciences, Gomal University, Dera Ismail Khan, Pakistan; 6School of Chemistry and Chemical Engineering, Sun Yat-Sen University, Guangzhou, People’s Republic of China; 7Interdisciplinary Research Centre in Biomedical Materials (IRCBM), COMSATS Institute of Information Technology, Lahore, Pakistan; 8School of Biotechnology, Jiangnan University, Wuxi, People’s Republic of China; 9Department of Chemistry, University of Science and Technology, Bannu, 10Department of Agriculture, University of Swabi, Swabi, Pakistan Abstract: Silver nanotechnology has received tremendous attention in recent years, owing to its wide range of applications in various fields and its intrinsic therapeutic properties. In this review, an attempt is made to critically evaluate the chemical, physical, and biological synthesis of silver nanoparticles (AgNPs) as well as their efficacy in the field of theranostics including microbiology and parasitology. Moreover, an outlook is also provided regarding the performance of AgNPs against different biological systems such as bacteria, fungi, viruses, and parasites (leishmanial and malarial parasites) in curing certain fatal human diseases, with a special focus on cancer. The mechanism of action of AgNPs in different biological systems still remains enigmatic. Here, due to limited available literature, we only focused on AgNPs mechanism in biological systems including human (wound healing and apoptosis), bacteria, and viruses which may open new windows for future research to ensure the versatile application of AgNPs in cosmetics, electronics, and medical fields. Keywords: synthesis of AgNPs, theranostics, antimicrobial properties, biomedical applications of AgNPs