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Quantitative phase imaging (QPI) aims at studying weakly scattering and absorbing biological specimens with subwavelength accuracy without any external staining mechanisms. Use of a reference beam at an angle is one of the necessary criteria for recording of high resolution holograms in most of the interferometric methods used for quantitative phase imaging. The spatial separation of the dc and twin images is decided by the reference beam angle and Fourier-filtered reconstructed image will have a very poor resolution if hologram is recorded below a minimum reference angle condition. However, it is always inconvenient to have a large reference beam angle while performing high resolution microscopy of live cells and biological specimens with nanometric features. In this paper, we treat reconstruction of digital holographic microscopy images as a constrained optimization problem with smoothness constraint in order to recover only complex object field in hologram plane even with overlapping dc and twin image terms. We solve this optimization problem by gradient descent approach iteratively and the smoothness constraint is implemented by spatial averaging with appropriate size. This approach will give excellent high resolution image recovery compared to Fourier filtering while keeping a very small reference angle. We demonstrate this approach on digital holographic microscopy of live cells by recovering the quantitative phase of live cells from a hologram recorded with nearly zero reference angle.

Source: ICHE Conference Archive - https://mdi-de.baw.de/icheArchive

A case report, the clinical features, and a histopathological report in one case of Hypereosinophilic syndrome (HES) is reported. This patient had exfoliative dermatitis, urticaria and angiodema. She had received treatment with steroids which showed improvement and recurrence after stopping steroid therapy. Unique feature of this case was that skin eruptions were the main presentation. Skin eruptions may be the only manifestation of otherwise asymptomatic patient of HES.

In the present work, static liquid morphologies confined to linear micron sized surface grooves were studied experimentally and analyzed theoretically. Geometries with increasing complexities, from wedges to trapezoidal grooves, were explored with the main focus on triangular grooves. In contrast to chemically structured substrates where only liquid morphologies with positive Laplace pressure are found, topographically structured substrates exhibit liquid morphologies with both positive and negative Laplace pressure. Depending upon the wettability and the exact geometry of substrates, either drop-like morphologies or elongated filaments with positive or negative Laplace pressure represent the generic equilibrium structures on the substrates. For very high contact angles, drop-like morphologies are dominant irrespective of the underlying substrate geometry. Transitions between these liquid morphologies can be triggered by varying the wettability or the geometry of substrates. In the present work, various cross sections of the grooves were explored while the wettability was controlled by various self-assembly monolayers or by means of the electrowetting effect. Upon changing the apparent contact angle of an aqueous drop by electrowetting, the transition between the drop-like and elongated filament morphologies could be triggered and thus a liquid can be transported along prefabricated grooves on demand. A clear threshold behavior for filling of the grooves was observed which corresponds to the stability boundaries of the static wetting morphologies in the respective groove geometry. The length of the liquid filament that advances into the groove depends on the exact geometry of the groove and the electrical properties of the system. An electrical model is presented to explain this behavior. Unlike liquid filaments in rectangular grooves, liquid filaments in triangular grooves become unstable when they are quenched from a filling into a non-filling regime. This instability of liquid filaments in triangular grooves was studied in detail using homogeneous filaments of glassy polymer (polystyrene) which have been prepared in a non equilibrium state by deposition from a solution. At elevated temperature, molten polystyrene restores its material contact angle with the substrate thus forming filaments with positive Laplace pressure. After dewetting, this liquid filament decays into isolated droplets with a characteristic spacing, depending upon wedge geometry, wettability and filament width. This instability is driven by the interplay of local filament width and Laplace pressure and constitutes a wide class of one-dimensional instabilities which also includes the Rayleigh - Plateau instability as a special case. The dynamics of this instability was also studied via in situ AFM experiments which allows to determine the time constant of the instability. A careful analysis of the time constant of the instability allows for the quantitative determination of the slip length in the system.