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The solid-state extraction technique and chromatography mass spectrometry were used to study the intrinsic composition of organic components of the mineral water obtained from the Munok deposit located in the Kazachinsko-Lensk district, Irkutsk region. The active components determining the mechanism of action of a mineral water-based preparation were identified and showed to operate similarly to those of Naftusya mineral water. The balneotherapeutic preparation was fabricated making use of the earlier developed technology of water vapor distillation. The analysis of organic components of mineral water and its extraction products showed the presence of aliphatic hydrocarbons with the high content of unsaturated compounds. The preparation thus obtained, unlike the standard balneotherapeutic medicines, does riot contain an inorganic matrix; it was shown to increase body resistance in the phase of remission in the patients treated for the management of urolithiasis.

The objective of the present work was to study the wound-healing potential of the new preparation, Extramin (a 32% ethanol extract of organic substances from Novonukutskaya mineral water) in a series of experiments on a model of chemical burns in rabbits. The wound healing process was monitored based on biochemical, hematiological, and morphological indicators. Analysis of the results allows for the conclusion that Extramin is a powerful stimulator of the wound-healing processes and can be recommended for further clinical studies.

A new technology for isolation of medicinal balneological preparations from mineral waters has been developed. It is based on the extraction of biologically active compounds (BAC) from mineral waters with ethanol following oxidative hydrolysis and water vapour distillation. This technology has no analogs both in this country and abroad. Unlike ordinary balneological medicines, those obtained by the new method do not contain inorganic matrix, show higher activity, and remain stable for a longer period. The primary composition of BACs isolated by the above technology was studied using solid-phase extraction and chromatomass-spectrometry. Analysis of Extramine, a medicinal preparation from Novonukutskaya hydrogen sulfide mineral water (Siberian Matsesta), allowed to identify active components responsible for its therapeutic effects and elucidate mechanisms of their action. The 15-year-long experience with practical application of BACs obtained with the help of the new technology has demonstrated that they possesses antiallergic, antiviral, and analeptic activities. These compounds increase general resistance of the organism to infection, promote removal of toxic substances, accelerate wound and injury healing, and prevent the development of herpes infection.

A male polar bear was caught at the Franz Josef Land at the age of 1 year and then lived in Moscow ZOO for 6 years. Thickening of the distal part of the right hind limb was noted in 1993. Destructive changes in the region of epymetaphysis were observed roentgenologically suggesting osteolytic osteosarcoma. The bear was euthanized and, at autopsy, the tumor had 15 cm in diameter and the distal parts of fibula and tibia were completely destroyed. Numerous cavities containing blood and necrotic areas were observed in the central parts of the tumor. The tumor was firm with hemorrhages in the peripheral parts. Multiple confluent metastases were found in all lobes of both lungs with no metastases in other organs. No radioactivity was found in the tumor tissue and bone at radiochemical analysis.