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21 results on '"Kim, Bieong-Kil"'

21. Synthesis of NBD-labeled DOTAP analog to track intracellular delivery of liposome.

Author

Doh KO, Kim BK, Lee TJ, Park JW, and Seu YB

Subjects
Animals, COS Cells, Chlorocebus aethiops, Fatty Acids, Monounsaturated chemistry, Fluorescent Dyes chemistry, Humans, Liposomes chemistry, MCF-7 Cells, Quaternary Ammonium Compounds chemistry, Endocytosis, Fatty Acids, Monounsaturated metabolism, Fluorescent Dyes metabolism, Liposomes metabolism, Quaternary Ammonium Compounds metabolism, Staining and Labeling
Abstract

A DOTAP analog labeled by NBD on the head group (DTNBD) was designed and synthesized to label DOTAP liposome. The structure was confirmed by (1)H NMR and FAB-MS, and the fluorescence of the newly synthesized DT-NBD was observed by fluorescent microscopy. The transfection efficiency of DOTAP liposome containing DT-NBD was comparable to commonly used NBD PE in COS7 and MCF7 cells. Furthermore, the level of cellular uptake and fluorescent intensity of fluorescent liposome containing DT-NBD was higher than NBD PE. Therefore, the novel NBD-labeled DOTAP analog seems to be effectively used for investigation of the cellular interaction and transfection mechanism of DOTAP liposome.

Published
2013
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