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Your search keyword '"Kim, Byeongyeon"' showing total 115 results
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115 results on '"Kim, Byeongyeon"'

14. Nanoporous electroporation needle for localized intracellular delivery in deep tissues.

Author

Lee, Gyeong Won, Kim, Byeongyeon, Lee, Tae Wook, Yim, Sang‐Gu, Chandrasekharan, Ajeesh, Kim, Hyewon, Choi, Sungyoung, and Yang, Seung Yun

Subjects
*ELECTROPORATION, *PROPIDIUM iodide, *TISSUES, *LABORATORY animals, *GENE transfection, *NEEDLES & pins
Abstract

The exogenous control of intracellular drug delivery has been shown to improve the overall efficacy of therapies by reducing nonspecific off‐target toxicity. However, achieving a precise on‐demand dosage of a drug in deep tissues with minimal damage is still a challenge. In this study, we report an electric‐pulse‐driven nanopore‐electroporation (nEP) system for the localized intracellular delivery of a model agent in deep tissues. Compared with conventional bulk electroporation, in vitro nEP achieved better transfection efficiency (>60%) with a high cell recovery rate (>95%) under a nontoxic low electroporation condition (40 V). Furthermore, in vivo nEP using a nanopore needle electrode with a side drug‐releasing compartment offered better control over the dosage release, time, and location of propidium iodide, which was used as a model agent for intracellular delivery. In a pilot study using experimental animals, the nEP system exhibited two times higher transfection efficiency of propidium iodide in the thigh muscle tissue, while minimizing tissue damage (<20%) compared to that of bulk electroporation. This tissue‐penetrating nEP platform can provide localized, safe, and effective intracellular delivery of diverse therapeutics into deep tissues in a controlled manner. [ABSTRACT FROM AUTHOR]

Published
2023
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30. Blood Divider for Simple, Surface Tension‐Based Isolation of Peripheral Blood Mononuclear Cells.

Author

Kim, Byeongyeon, Seo, In‐Ho, Seo, Gwang Myeong, Kim, Woo‐Joong, Shin, Eui‐Cheol, and Choi, Sungyoung

Subjects
*MONONUCLEAR leukocytes, *DENSITY gradient centrifugation, *BLOOD cells
Abstract

Peripheral blood mononuclear cell (PBMC) separation is routinely performed by centrifugation with a density gradient medium, and is essential for immunological research and clinical applications. PBMC separation highly relies on laborious, time‐consuming blood layering and PBMC collection by careful pipetting. Current methods to address this problem use a physical barrier formed between the separation medium and a blood sample to avoid the need for delicate pipetting, but still pose a risk of red blood cell (RBC) contamination. Here, a blood divider (B‐DV) is proposed as a centrifuge tube insert that allows blood cells to pass through a separation medium in one direction, while blocking fluid passage in the opposite direction and thus preventing RBC contamination during PBMC harvest. The B‐DV is a 3D‐printed mesh in which a fluid can divide into two, above and below the mesh face as soon as inverting the centrifuge tube. Such complete fluid division enables minimizing the volume of the separation medium to the extent of RBC volume to be packed, thus reducing the sedimentation distance for RBCs, and facilitating rapid PBMC isolation by simple tube inverting. This technology enables rapid, easy PBMC separation without user‐to‐user variability on PBMC separation efficiencies. [ABSTRACT FROM AUTHOR]

Published
2022
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42. Super-Resolution Three-Dimensional Imaging of Actin Filaments in Cultured Cells and the Brain viaExpansion Microscopy

Author

Park, Chan E, Cho, Youngbin, Cho, In, Jung, Hyunsu, Kim, Byeongyeon, Shin, Jennifer H., Choi, Sungyoung, Kwon, Seok-Kyu, Hahn, Young Ki, and Chang, Jae-Byum

Abstract

Actin is an essential protein in almost all life forms. It mediates diverse biological functions, ranging from controlling the shape of cells and cell movements to cargo transport and the formation of synaptic connections. Multiple diseases are closely related to the dysfunction of actin or actin-related proteins. Despite the biological importance of actin, super-resolution imaging of it in tissue is still challenging, as it forms very dense networks in almost all cells inside the tissue. In this work, we demonstrate multiplexed super-resolution volumetric imaging of actin in both cultured cells and mouse brain slices viaexpansion microscopy (ExM). By introducing a simple labeling process, which enables the anchoring of an actin probe, phalloidin, to a swellable hydrogel, the multiplexed ExM imaging of actin filaments was achieved. We first showed that this technique could visualize the nanoscale details of actin filament organizations in cultured cells. Then, we applied this technique to mouse brain slices and visualized diverse actin organizations, such as the parallel actin filaments along the long axis of dendrites and dense actin structures in postsynaptic spines. We examined the postsynaptic spines in the mouse brain and showed that the organizations of actin filaments are highly diverse. This technique, which enables the high-throughput 60 nm resolution imaging of actin filaments and other proteins in cultured cells and thick tissue slices, would be a useful tool to study the organization of actin filaments in diverse biological circumstances and how they change under pathological conditions.

Published
2020
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