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12. Novel GSK-3β Inhibitor Neopetroside A Protects Against Murine Myocardial Ischemia/Reperfusion Injury

Author

Kim, Hyoung Kyu, Kim, Min, Marquez, Jubert C., Jeong, Seung Hun, Ko, Tae Hee, Noh, Yeon Hee, Kha, Pham Trong, Choi, Ha Min, Kim, Dong Hyun, Kim, Jong Tae, Yang, Young Il, Ko, Kyung Soo, Rhee, Byoung Doo, Shubina, Larisa K., Makarieva, Tatyana N., Yashunsky, Dmitry Y., Gerbst, Alexey G., Nifantiev, Nikolay E., Stonik, Valentin A., and Han, Jin

Published
2022
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14. NEK2 Phosphorylates RhoGDI1 to Promote Cell Proliferation, Migration and Invasion Through the Activation of RhoA and Rac1 in Colon Cancer Cells.

Author

Lim, Jeewon, Hwang, Yo-Sep, Kim, Jong-Tae, Yoon, Hyang-Ran, Park, Hyo-Min, Han, Jahyeong, Kwon, Taeho, Lee, Kyung-Ho, Cho, Hee-Jun, and Lee, Hee-Gu

Subjects
CANCER cell motility, CANCER cell proliferation, CANCER cell migration, RHO GTPases, CELL migration
Abstract

Rho guanine nucleotide dissociation inhibitor 1 (RhoGDI1) plays a critical role in regulating the activity of Rho guanosine triphosphatases (GTPases). Phosphorylation of RhoGDI1 dynamically modulates the activation of Rho GTPases, influencing cell proliferation and migration. This study explored the involvement of Never In Mitosis A (NIMA)-related serine/threonine protein kinase 2 (NEK2) in phosphorylating RhoGDI1 and its implications in cancer cell behavior associated with tumor progression. We employed GST pull-down assays and immunoprecipitation to investigate the interaction between NEK2 and RhoGDI1. Truncation fragments identified the region of RhoGDI1 responsible for binding with NEK2. Phosphorylation assays determined the site of NEK2-mediated phosphorylation on RhoGDI1. Functional assays were conducted using overexpression of the RhoGDI1 substitution mutant to assess their impact on cancer cell behavior. NEK2 directly bound to RhoGDI1 and phosphorylated it at Ser174. This phosphorylation event facilitated cancer cell proliferation and motility by activating RhoA and Rac1. The RhoGDI1 aa 112–134 region was critical for the binding to NEK2. Disruption of the NEK2–RhoGDI1 interaction through overexpression of a RhoGDI1 truncated fragment (aa 112–134) led to diminished RhoGDI1 phosphorylation and RhoA/Rac1 activation induced by NEK2, resulting in reduced cancer cell proliferation and migration. Moreover, in vivo studies showed reduced tumor growth and lung metastasis when the NEK2–RhoGDI1 interaction was disrupted. This study indicates that NEK2 promotes the metastatic behaviors of cancer cells by activating RhoA and Rac1 by phosphorylating RhoGDI1. [ABSTRACT FROM AUTHOR]

Published
2024
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24. Study of the interactions of pions in the CALICE silicon-tungsten calorimeter prototype

Author

Adloff, C., Karyotakis, Y., Repond, J., Yu, J., Eigen, G., Mikami, Y., Watson, N. K., Wilson, J. A., Goto, T., Mavromanolakis, G., Thomson, M. A., Ward, D. R., Yan, W., Benchekroun, D., Hoummada, A., Khoulaki, Y., Apostolakis, J., Ribon, A., Uzhinskiy, V., Benyamna, M., Cârloganu, C., Fehr, F., Gay, P., Blazey, G. C., Chakraborty, D., Dyshkant, A., Francis, K., Hedin, D., Lima, J. G., Zutshi, V., Hostachy, J. -Y., Krastev, K., Morin, L., D'Ascenzo, N., Cornett, U., David, D., Fabbri, R., Falley, G., Gadow, K., Garutti, E., Göttlicher, P., Jung, T., Karstensen, S., Lucaci-Timoce, A. -I., Lutz, B., Meyer, N., Morgunov, V., Reinecke, M., Sefkow, F., Smirnov, P., Vargas-Trevino, A., Wattimena, N., Wendt, O., Feege, N., Groll, M., Haller, J., Heuer, R. -D., Morozov, S., Richter, S., Samson, J., Kaplan, A., Schultz-Coulon, H. -Ch., Shen, W., Tadday, A., Bilki, B., Norbeck, E., Onel, Y., Kim, E. J., Kim, G., Kim, D-W., Lee, K., Lee, S. C., Kawagoe, K., Tamura, Y., Dauncey, P. D., Magnan, A. -M., Yilmaz, H., Zorba, O., Bartsch, V., Postranecky, M., Warren, M., Wing, M., Green, M. G., Salvatore, F., Bedjidian, M., Kieffer, R., Laktineh, I., Fouz, M. -C., Bailey, D. S., Barlow, R. J., Kelly, M., Thompson, R. J., Danilov, M., Tarkovsky, E., Baranova, N., Karmanov, D., Korolev, M., Merkin, M., Voronin, A., Frey, A., Lu, S., Seidel, K., Simon, F., Soldner, C., Weuste, L., Bonis, J., Bouquet, B., Callier, S., Cornebise, P., Doublet, Ph., Giannelli, M. Faucci, Fleury, J., Li, H., Martin-Chassard, G., Richard, F., de la Taille, Ch., Poeschl, R., Raux, L., Seguin-Moreau, N., Wicek, F., Anduze, M., Boudry, V., Brient, J-C., Gaycken, G., Jeans, D., de Freitas, P. Mora, Musat, G., Reinhard, M., Rougé, A., Ruan, M., Vanel, J-Ch., Videau, H., Park, K-H., Zacek, J., Cvach, J., Gallus, P., Havranek, M., Janata, M., Marcisovsky, M., Polak, I., Popule, J., Tomasek, L., Tomasek, M., Ruzicka, P., Sicho, P., Smolik, J., Vrba, V., Zalesak, J., Belhorma, B., Belmir, M., Nam, S. W., Park, I. H., Yang, J., Chai, Jong-Seo, Kim, Jong-Tae, Kim, Geun-Bum, Kang, J., and Kwon, Y. -J.

Subjects
Physics - Instrumentation and Detectors, High Energy Physics - Experiment
Abstract

A prototype silicon-tungsten electromagnetic calorimeter for an ILC detector was tested in 2007 at the CERN SPS test beam. Data were collected with electron and hadron beams in the energy range 8 to 80 GeV. The analysis described here focuses on the interactions of pions in the calorimeter. One of the main objectives of the CALICE program is to validate the Monte Carlo tools available for the design of a full-sized detector. The interactions of pions in the Si-W calorimeter are therefore confronted with the predictions of various physical models implemented in the GEANT4 simulation framework.

Published
2010
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25. Construction and Commissioning of the CALICE Analog Hadron Calorimeter Prototype

Author

Adloff, C., Karyotakis, Y., Repond, J., Brandt, A., Brown, H., De, K., Medina, C., Smith, J., Li, J., Sosebee, M., White, A., Yu, J., Buanes, T., Eigen, G., Mikami, Y., Miller, O., Watson, N. K., Wilson, J. A., Goto, T., Mavromanolakis, G., Thomson, M. A., Ward, D. R., Yan, W., Benchekroun, D., Hoummada, A., Khoulaki, Y., Oreglia, M., Benyamna, M., Cârloganu, C., Gay, P., Ha, J., Blazey, G. C., Chakraborty, D., Dyshkant, A., Francis, K., Hedin, D., Lima, G., Zutshi, V., Babkin, V. A., Bazylev, S. N., Fedotov, Yu. I., Slepnev, V. M., Tiapkin, I. A., Volgin, S. V., Hostachy, J. -Y., Morin, L., D?Ascenzo, N., Cornett, U., David, D., Fabbri, R., Falley, G., Feege, N., Gadow, K., Garutti, E., Göttlicher, P., Jung, T., Karstensen, S., Korbel, V., Lucaci-Timoce, A. -I., Lutz, B., Meyer, N., Morgunov, V., Reinecke, M., Schätzel, S., Schmidt, S., Sefkow, F., Smirnov, P., Vargas-Trevino, A., Wattimena, N., Wendt, O., Groll, M., Heuer, R. -D., Richter, S., Samson, J., Kaplan, A., Schultz-Coulon, H. -Ch., Shen, W., Tadday, A., Bilki, B., Norbeck, E., Onel, Y., Kim, E. J., Kim, G., Kim, D-W., Lee, K., Lee, S. C., Kawagoe, K., Tamura, Y., Ballin, J. A., Dauncey, P. D., Magnan, A. -M., Yilmaz, H., Zorba, O., Bartsch, V., Postranecky, M., Warren, M., Wing, M., Giannelli, M. Faucci, Green, M. G., Salvatore, F., Kieffer, R., Laktineh, I., Fouz, M. C, Bailey, D. S., Barlow, R. J., Thompson, R. J., Batouritski, M., Dvornikov, O., Shulhevich, Yu., Shumeiko, N., Solin, A., Starovoitov, P., Tchekhovski, V., Terletski, A., Bobchenko, B., Chadeeva, M., Danilov, M., Markin, O., Mizuk, R., Novikov, E., Rusinov, V., Tarkovsky, E., Andreev, V., Kirikova, N., Komar, A., Kozlov, V., Soloviev, Y., Terkulov, A., Buzhan, P., Dolgoshein, B., Ilyin, A., Kantserov, V., Kaplin, V., Karakash, A., Popova, E., Smirnov, S., Baranova, N., Boos, E., Gladilin, L., Karmanov, D., Korolev, M., Merkin, M., Savin, A., Voronin, A., Topkar, A., Freyk, A., Kiesling, C., Lu, S., Prothmann, K., Seidel, K., Simon, F., Soldner, C., Weuste, L., Bouquet, B., Callier, S., Cornebise, P., Dulucq, F., Fleury, J., Li, H., Martin-Chassard, G., Richard, F., de la Taille, Ch., Poeschl, R., Raux, L., Ruan, M., Seguin-Moreau, N., Wicek, F., Anduze, M., Boudry, V., Brient, J-C., Gaycken, G., Cornat, R., Jeans, D., de Freitas, P. Mora, Musat, G., Reinhard, M., Rougé, A., Vanel, J-Ch., Videau, H., Park, K-H., Zacek, J., Cvach, J., Gallus, P., Havranek, M., Janata, M., Kvasnicka, J., Marcisovsky, M., Polak, I., Popule, J., Tomasek, L., Tomasek, M., Ruzicka, P., Sicho, P., Smolik, J., Vrba, V., Zalesak, J., Arestov, Yu., Ammosov, V., Chuiko, B., Gapienko, V., Gilitski, Y., Koreshev, V., Semak, A., Sviridov, Yu., Zaets, V., Belhorma, B., Belmir, M., Baird, A., Halsall, R. N., Nam, S. W., Park, I. H., Yang, J., Chai, Jong-Seo, Kim, Jong-Tae, Kim, Geun-Bum, Kim, Y., Kang, J., Kwon, Y. -J., Kim, Ilgoo, Lee, Taeyun, Park, Jaehong, Sung, Jinho, Itoh, S., Kotera, K., Nishiyama, M., Takeshita, T., Weber, S., and Zeitnitz, C.

Subjects
Physics - Instrumentation and Detectors, High Energy Physics - Experiment
Abstract

An analog hadron calorimeter (AHCAL) prototype of 5.3 nuclear interaction lengths thickness has been constructed by members of the CALICE Collaboration. The AHCAL prototype consists of a 38-layer sandwich structure of steel plates and highly-segmented scintillator tiles that are read out by wavelength-shifting fibers coupled to SiPMs. The signal is amplified and shaped with a custom-designed ASIC. A calibration/monitoring system based on LED light was developed to monitor the SiPM gain and to measure the full SiPM response curve in order to correct for non-linearity. Ultimately, the physics goals are the study of hadron shower shapes and testing the concept of particle flow. The technical goal consists of measuring the performance and reliability of 7608 SiPMs. The AHCAL was commissioned in test beams at DESY and CERN. The entire prototype was completed in 2007 and recorded hadron showers, electron showers and muons at different energies and incident angles in test beams at CERN and Fermilab., Comment: 36 pages, 32 figures

Published
2010
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26. Therapeutic Effect of Donepezil on Neuroinflammation and Cognitive Impairment after Moderate Traumatic Brain Injury.

Author

Youn, Dong Hyuk, Lee, Younghyurk, Han, Sung Woo, Kim, Jong-Tae, Jung, Harry, Han, Gui Seung, Yoon, Jung In, Lee, Jae Jun, and Jeon, Jin Pyeong

Subjects
TREATMENT effectiveness, BRAIN injuries, REACTIVE oxygen species, MICROTUBULE-associated proteins, BAX protein
Abstract

Background: Despite the important clinical issue of cognitive impairment after moderate traumatic brain injury (TBI), there is currently no suitable treatment. Here, we used in vitro and in vivo models to investigate the effect of Donepezil—an acetylcholinesterase (AChE) inhibitor—on cognitive impairment in the acute period following injury, while focusing on neuroinflammation and autophagy- and mitophagy-related markers. Methods: The purpose of the in vitro study was to investigate potential neuroprotective effects in TBI-induced cells after donepezil treatment, and the in vivo study, the purpose was to investigate therapeutic effects on cognitive impairment in the acute period after injury by analyzing neuroinflammation and autophagy- and mitophagy-related markers. The in vitro TBI model involved injuring SH-SY5Y cells using a cell-injury controller and then investigating the effect of donepezil at a concentration of 80 μM. The in vivo TBI model was made using a stereotaxic impactor for male C57BL/6J mice. Immuno-histochemical markers and cognitive functions were compared after 7 days of donepezil treatment (1 mg/kg/day). Mice were divided into four groups: sham operation with saline treatment, sham operation with donepezil treatment, TBI with saline treatment, and TBI with donepezil treatment (18 mice in each group). Donepezil treatment was administered within 4 h post-TBI. Results: In vitro, donepezil was found to lead to increased cell viability and 5,5′,6,6′-tetrachloro-1,1′,3,3′-tetraethylbenzimi-dazolylcarbocyanine iodide (JC-1), along with decreased reactive oxygen species (ROS), lactate-dehydrogenase (LDH), 2′-7′-dichlorodihydrofluorescein diacetate (DCFH-DA)-positive cells, and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-positive cells. The mRNA and protein expressions of neuroinflammation (Cyclooxygenase-2, COX-2; NOD-like receptor protein 3, NLRP3; Caspase-1; and Interleukin-1 beta, IL-1β), as well as autophagy- and mitophagy-related markers (death-associated protein kinase 1, DAPK1; PTEN-induced kinase 1, PINK1; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like, BNIP3L; Beclin-1, BECN1; BCL2-associated X protein, BAX; microtubule-associated protein 1A/1B-light chain 3B (LC3B); Sequestosome-1; and p62) were all found to decrease after donepezil treatment. The in vivo study also showed that donepezil treatment resulted in decreased levels of cortical tissue losses and brain swelling in TBI compared to the TBI group without donepezil treatment. Donepezil treatment was also shown to decrease the mRNA and Western blotting expressions of all markers, and especially COX-2 and BNIP3L, which showed the most significant decreases. Moreover, TBI mice showed an decreased escape latency, increased alteration rate, and improved preference index, altogether pointing to better cognitive performance after donepezil treatment. Conclusions: Donepezil treatment may be beneficial in improving cognitive impairment in the early phase of moderate traumatic brain injury by ameliorating neuroinflammation, as well as autophagy and mitophagy. [ABSTRACT FROM AUTHOR]

Published
2024
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