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Supergenes maintain adaptive clusters of alleles in the face of genetic mixing. Although usually attributed to inversions, supergenes can be complex, and reconstructing the precise processes that led to recombination suppression and their timing is challenging. We investigated the origin of the BC supergene, which controls variation in warning coloration in the African monarch butterfly, Danaus chrysippus. By generating chromosome-scale assemblies for all three alleles, we identified multiple structural differences. Most strikingly, we find that a region of more than 1 million bp underwent several segmental duplications at least 7.5 Ma. The resulting duplicated fragments appear to have triggered four inversions in surrounding parts of the chromosome, resulting in stepwise growth of the region of suppressed recombination. Phylogenies for the inversions are incongruent with the species tree and suggest that structural polymorphisms have persisted for at least 4.1 Myr. In addition to the role of duplications in triggering inversions, our results suggest a previously undescribed mechanism of recombination suppression through independent losses of divergent duplicated tracts. Overall, our findings add support for a stepwise model of supergene evolution involving a variety of structural changes. This article is part of the theme issue Genomic architecture of supergenes: causes and evolutionary consequences.

For ultra-high-speed digital transmission, required by 5G/6G communications, ultra-wideband common-mode rejection (CMR) structures with autonomous phase-balancing capability are proposed. Common-mode noise, caused by phase and amplitude unbalances, is one of the most undesired disturbances affecting modern digital circuits. According to the circuit design guides with a typically used differential line (DL) for high-speed digital transmission, common-mode rejection is achieved using CMR filters, and the unbalanced phase, caused by a length difference between the two signal lines of a DL, is compensated by inserting an additional delay line. However, due to nonlinear phase interactions between the two DLs and unbalanced electromagnetic (EM) interferences, the conventional compensation method is frequency-limited at around 10 GHz. To significantly enhance the common-mode rejection level and extend the phase recovery bandwidth, the proposed CMR structure utilizes a planar balanced line (BL), such as a coplanar stripline (CPS) or a parallel stripline (PSL), along with additional conductor strips arranged laterally near the BL. To demonstrate the performance of the proposed BL-based CMR structures, various types of CMR structures are fabricated, and the measurement results are compared with the 3D EM simulation results. As a result, it is proven that the proposed BL-based CMR structures have the capability to reject the common-mode noise with suppression levels of more than 10 dB and to simultaneously recover the phase balance from near DC to over 40 GHz. [ABSTRACT FROM AUTHOR]

An absolute timescale for evolution is essential if we are to associate evolutionary phenomena, such as adaptation or speciation, with potential causes, such as geological activity or climatic change. Timescales in most phylogenetic studies use geologically dated fossils or phylogeographic events as calibration points, but more recently, it has also become possible to use experimentally derived estimates of the mutation rate as a proxy for substitution rates. The large radiation of drosophilid taxa endemic to the Hawaiian islands has provided multiple calibration points for the Drosophila phylogeny, thanks to the "conveyor belt" process by which this archipelago forms and is colonized by species. However, published date estimates for key nodes in the Drosophila phylogeny vary widely, and many are based on simplistic models of colonization and coalescence or on estimates of island age that are not current. In this study, we use new sequence data from seven species of Hawaiian Drosophila to examine a range of explicit coalescent models and estimate substitution rates. We use these rates, along with a published experimentally determined mutation rate, to date key events in drosophilid evolution. Surprisingly, our estimate for the date for the most recent common ancestor of the genus Drosophila based on mutation rate (25-40 Ma) is closer to being compatible with independent fossil-derived dates (20-50 Ma) than are most of the Hawaiian-calibration models and also has smaller uncertainty. We find that Hawaiian-calibrated dates are extremely sensitive to model choice and give rise to point estimates that range between 26 and 192 Ma, depending on the details of the model. Potential problems with the Hawaiian calibration may arise from systematic variation in the molecular clock due to the long generation time of Hawaiian Drosophila compared with other Drosophila and/or uncertainty in linking island formation dates with colonization dates. As either source of error will bias estimates of divergence time, we suggest mutation rate estimates be used until better models are available.

A design method for an ultra-wideband coplanar-stripline-based vertical transition that can be used for ultra-high-speed digital interfaces is proposed. A conventional via structure, based on a differential line (DL), inherently possesses performance limitations (<10 GHz) due to difficulties in maintaining constant line impedance and smooth electric field transformation, in addition to the effects of signal skews, FR4 fiber weave, and unbalanced EM interferences. DL-based digital interfaces may not meet the demands of ultra-high-speed digital data transmission required for the upcoming 6G communications. The use of a coplanar stripline (CPS), a type of planar balanced line (BL), for the vertical transition, along with the ultra-wideband DL-to-CPS transition, mostly removes the inherent and unfavorable issues of the DL and enables ultra-high-speed digital data transmission. The design process of the transition is simplified using the analytical design formulas, derived using the conformal mapping method, of the transition. The characteristic line impedances of the transition are calculated and found to be in close agreement with the results obtained from EM simulations. Utilizing these results, the CPS-based vertical transition, maintaining the characteristic line impedance of 100 Ω, is designed and fabricated. The measured results confirm its ultra-wideband characteristics, with a maximum of 1.6 dB insertion loss and more than 10 dB return loss in the frequency range of DC to 30 GHz. Therefore, the proposed CPS-based vertical transition offers a significantly wider frequency bandwidth, i.e., more than three times that of conventional DL-based via structures. [ABSTRACT FROM AUTHOR]

Ultra-wideband (UWB) antennas cover a frequency range of 3.1 to 10.6 GHz and have sparked a lot of research interest as an essential part of wireless communication systems as they provide high data transmission speeds, are less expensive, and consume less power. UWB antennas are widely used in radar imaging, radio frequency identification, public security, and other high-accuracy positioning devices such as altimetry. Some smart applications of UWB antennas are vehicular radar systems, surveillance systems, software-defined radios, spectrum analysis, proximity fuses, etc. Multiple-input-multiple-output (MIMO) is a multiplexing technology that adopts multiple antennas both at the transmitter and receiver, which can enhance the channel capacity. MIMO technology is extensively used in several applications, such as in portable devices, wireless body area networks (WBANs), vehicular communication, and satellite–terrestrial networks. Generally, the MIMO antennas are used to obtain high reliability, high capacity, high throughput, and high security. The UWB MIMO antennas (UMAs) are considered the best choice for wireless communication systems as they offer reliability and wide transmission capacity, in contrast to unit antenna elements (AEs), without increasing system bandwidth or transmission power. The present-day and future communications systems need higher throughput to meet the demands of users. The signal transfer rate can be improved by improving channel bandwidth or increasing the number of receiving antennas. However, the main issue in designing UMAs is to provide high isolation between AEs because mutual coupling interactions between them affect the generated radiation patterns, leading to worse performance and failing to meet the operative constraints and requirements. When introducing decoupling techniques (DTs), researchers experience numerous challenges, including an increase in antenna size, design complexity, and cross-polarization. This article offers an organized review and simulated study of the various DTs in UMAs. The simulated study has been carried out through the implementation of various types of DTs on the same two-port UMA, which consists of two microstrip-fed circular-shaped AEs with open-end slotted partial ground. In contrast with previously reported review articles, this article provides a detailed study of various types of DTs reported so far and a better understanding for selecting appropriate DTs, which help in designing UMAs with better performance. [ABSTRACT FROM AUTHOR]

Polymer electrolytes based on poly (ethylene oxide) and ionic liquid (IL), 1-methyl 3-propyl imidazolium iodide (PMII) were prepared in film form by solution casting technique. Atomic force microscope (AFM) picture confirms the high surface roughness of films. The maximum ionic conductivity of the polymer /IL/[I.sub.2] electrolyte was 3.56 x [10.sup.-4] S [cm.sup.-1] at room temperature. Doping of iodine in humid atmosphere formed 'fractal like structure' which affected ionic conductivity. The dyesensitized solar cell (DSSC) using maximum ionic conductivity solid film at different humidity shows promising results. POLYM. ENG. SCI., 49:862-865, 2009. ©2009 Society of Plastics Engineers, INTRODUCTION Dye-sensitized solar cell (DSSC), as an alternate source in place of photo electrochemical solar cells, came in consideration after the novel work of Gratzel and co-workers (1), (2). The [...]

In natural populations, genetic variation affects resistance to disease. Knowing how much variation exists, and understanding the genetic architecture of this variation, is important for medicine, for agriculture, and for understanding evolutionary processes. To investigate the extent and nature of genetic variation affecting resistance to pathogens, we are studying a tractable model system: Drosophila melanogaster and its natural pathogen the vertically transmitted sigma virus. We show that considerable genetic variation affects transmission of the virus from parent to offspring. However, maternal and paternal transmission of the virus is affected by different genes. Maternal transmission is a simple Mendelian trait: most of the genetic variation is explained by a polymorphism in ref(2)P, a gene already well known to affect resistance to sigma. In contrast, there is considerable genetic variation in paternal transmission that cannot be explained by ref(2)P and is caused by other loci on chromosome 2. Furthermore, we found no genetic correlation between paternal transmission of the virus and resistance to infection by the sigma virus following injection. This suggests that different loci affect viral replication and paternal transmission.

An essential component of the immune system of animals is the production of antimicrobial peptides (AMPs). In vertebrates and termites the protein sequence of some AMPs evolves rapidly under positive selection, suggesting that they may be coevolving with pathogens. However, antibacterial peptides in Drosophila tend to be highly conserved. We have inferred the selection pressures acting on Drosophila antifungal peptides (drosomycins) from both the divergence of drosomycin genes within and between five species of Drosophila and polymorphism data from Drosophila simulans and D. melanogaster. In common with Drosophila antibacterial peptides, there is no evidence of adaptive protein evolution in any of the drosomycin genes, suggesting that they do not coevolve with pathogens. It is possible that this reflects a lack of specific fungal and bacterial parasites in Drosophila populations. The polymorphism data from both species differed from neutrality at one locus, but this was not associated with changes in the protein sequence. The synonymous site diversity was greater in D. simulans than in D. melanogaster, but the diversity both upstream of the genes and at nonsynonymous sites was similar. This can be explained if both upstream and nonsynonymous mutations are slightly deleterious and are removed more effectively from D. simulans due to its larger effective population size.