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(1) Background: The aim of this study was to investigate the circadian rhythms of tongue features according to the effects of physiological phases over a 24 h period. (2) Methods: Fifteen healthy participants aged 20 to 69 years were recruited. The participants did not have current chronic diseases or past diseases and had to meet the inclusion and exclusion criteria. The participants stayed at the Gil Hospital for a duration of 2 nights and 3 days. On the first day, at 18:00, they consumed their allocated portions of food and water and then completed a questionnaire. At approximately 21:00, their tongue images were acquired using a computerized tongue image acquisition system, following which they slept for 8 h, commencing at 23:00. Measurements were taken from 07:00 through 21:00 on the second day, and the final acquisition was taken at 07:00 on the following morning, resulting in a total of eight images. The circadian rhythm was authenticated and quantified utilizing the single cosinor analysis, a technique for periodic regression analysis for fitting a 24 h cosine curve. (3) Results: Cosinor analysis revealed that all tongue features were significantly related to circadian rhythm. (4) Conclusions: The results of this study may be important for considering the time of day at which the tongue is observed and tongue status is evaluated. [ABSTRACT FROM AUTHOR]

The design and fabrication of a novel microfluidic nanoprobe system are presented. The nanoprobe consists of cantilevered ultrasharp volcano-like tips, with microfluidic capabilities consisting of microchannels connected to an on-chip reservoir. The chip possesses additional connection capabilities to a remote reservoir. The fabrication uses standard surface micromachining techniques and materials. Bulk micromachining is employed for chip release. The microchannels are fabricated in silicon nitride by a new methodology, based on edge underetching of a sacrificial layer, bird's beak oxidation for mechanically closing the edges, and deposition of a sealing layer. The design and integration of various elements of the system and their fabrication are discussed. The system is conceived mainly to work as a 'nanofountain pen', i.e., a continuously writing upgrade of the dip-pen nanolithography approach. Moreover, the new chip shows a much larger applicability area in fields such as electrochemical nanoprobes, nanoprobe-based etching, build-up tools for nanofabrication, or a probe for materials interactive analysis. Preliminary tests for writing and imaging with the new device were performed. These tests illustrate the capabilities of the new device and demonstrate possible directions for improvement. [1517] Index Terms--Dip-pen nanolithography, microfluidics, micropipette, nanoprobe.