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1,123 results on '"Kim, Min Woo"'

4. Identification of direct connections between the dura and the brain

Author

Smyth, Leon C. D., Xu, Di, Okar, Serhat V., Dykstra, Taitea, Rustenhoven, Justin, Papadopoulos, Zachary, Bhasiin, Kesshni, Kim, Min Woo, Drieu, Antoine, Mamuladze, Tornike, Blackburn, Susan, Gu, Xingxing, Gaitán, María I., Nair, Govind, Storck, Steffen E., Du, Siling, White, Michael A., Bayguinov, Peter, Smirnov, Igor, Dikranian, Krikor, Reich, Daniel S., and Kipnis, Jonathan

Published
2024
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29. Luciferase-LOV BRET enables versatile and specific transcriptional readout of cellular protein-protein interactions

Author

Kim, Christina K, Cho, Kelvin F, Kim, Min Woo, and Ting, Alice Y

Subjects
Biochemistry and Cell Biology, Biological Sciences, Genetics, Generic health relevance, Cytological Techniques, Genes, Reporter, HEK293 Cells, Humans, Light, Luciferases, Molecular Biology, Protein Interaction Mapping, Sensitivity and Specificity, BRET, LOV domain, cell biology, human embryonic kidneys 293 cells, luciferase, protein-protein interactions, Biological sciences, Biomedical and clinical sciences, Health sciences
Abstract

Technologies that convert transient protein-protein interactions (PPIs) into stable expression of a reporter gene are useful for genetic selections, high-throughput screening, and multiplexing with omics technologies. We previously reported SPARK (Kim et al., 2017), a transcription factor that is activated by the coincidence of blue light and a PPI. Here, we report an improved, second-generation SPARK2 that incorporates a luciferase moiety to control the light-sensitive LOV domain. SPARK2 can be temporally gated by either external light or addition of a small-molecule luciferin, which causes luciferase to open LOV via proximity-dependent BRET. Furthermore, the nested 'AND' gate design of SPARK2-in which both protease recruitment to the membrane-anchored transcription factor and LOV domain opening are regulated by the PPI of interest-yields a lower-background system and improved PPI specificity. We apply SPARK2 to high-throughput screening for GPCR agonists and for the detection of trans-cellular contacts, all with versatile transcriptional readout.

Published
2019

34. Ultra‐strong and biodegradable nanocomposite fibers of poly(butylene adipate‐co‐terephthalate)/cellulose nanocrystal prepared by dry‐jet wet spinning.

Author

Lee, Youngeun, Kim, Min Woo, Kim, Hyo Jeong, Kim, Jin Kyung, Won, Tae Kyung, Miyawaki, Jin, Chae, Han Gi, and Eom, Youngho

Subjects
*CELLULOSE nanocrystals, *NYLON fibers, *CHEMICAL affinity, *FIBERS, *BUTENE, *NANOCOMPOSITE materials, *CELLULOSE, *POLYESTER fibers
Abstract

Fiber‐based products constitute a significant portion of plastic waste and cause environmental damage. In particular, discarded sanitary masks and fishing gear disintegrate into microfiber plastics, posing a significant threat to human health and ecosystems. In this study, we developed robust biodegradable nanocomposite fibers of poly(butylene adipate‐co‐terephthalate) (PBAT)/cellulose nanocrystals (CNCs) (1 and 2 wt%) through dry‐jet wet spinning, by using dimethyl sulfoxide (DMSO) as a common solvent. The control PBAT fibers exhibit remarkable mechanical performance with tensile strength and toughness of 160.0 MPa and 43.0 MJ m−3, respectively. CNC addition has a toughening effect with slightly reduced strength but enhanced toughness (148.8 MPa and 69.0 MJ m−3, respectively, for 2 wt% CNC); their mechanical performances are superior to those of previously reported PBAT‐based materials. The remarkable performance of the fibers is attributed to a highly oriented structure with a total draw ratio of 15 after post‐hot drawing. The control and nanocomposite fibers exhibit spot‐like patterns in 2D wide‐angle x‐ray diffraction patterns with Herman's orientation factor of 0.54–0.58. The theoretical Hansen solubility parameter confirmed the poor chemical affinity between the PBAT and CNC. Nonetheless, the rheological characterization revealed that well‐dispersed CNCs with DMSO produced a physical network in the PBAT matrix, resulting in the toughening effect. Such robust nanocomposite fibers consisting of fully biodegradable components are promising alternatives to nondegradable nylon and polyester fibers. Highlights: Robust biodegradable nanocomposite fibers of PBAT and CNC are prepared.Nanocomposite fibers are dry‐jet wet spun using DMSO as a common solvent.PBAT fibers exhibit strength and toughness of 160.0 MPa and 43.0 MJ m−3.2 wt% CNC toughens the PBAT fibers with an enhanced toughness of 69.0 MJ m−3.Rheological results confirm the toughening effect of well‐dispersed CNC in PBAT. [ABSTRACT FROM AUTHOR]

Published
2024
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35. Mechanisms of Groucho-mediated repression revealed by genome-wide analysis of Groucho binding and activity

Author

Chambers, Michael, Turki-Judeh, Wiam, Kim, Min Woo, Chen, Kenny, Gallaher, Sean D, and Courey, Albert J

Subjects
Biochemistry and Cell Biology, Genetics, Biological Sciences, Human Genome, Biotechnology, 2.1 Biological and endogenous factors, Aetiology, 1.1 Normal biological development and functioning, Underpinning research, Generic health relevance, Animals, Basic Helix-Loop-Helix Transcription Factors, Chromatin, Drosophila melanogaster, Embryo, Nonmammalian, Gene Expression Regulation, Developmental, Genomics, Protein Binding, Repressor Proteins, Groucho, Transcriptional repression, Drosophila embryogenesis, ChIP-seq, RNA-seq, Chromatin-associated RNA-seq, RNA polymerase II pausing, Chromatin-associated RNA-seq, RNA polymerase II pausing, Information and Computing Sciences, Medical and Health Sciences, Bioinformatics, Biological sciences, Biomedical and clinical sciences
Abstract

BackgroundThe transcriptional corepressor Groucho (Gro) is required for the function of many developmentally regulated DNA binding repressors, thus helping to define the gene expression profile of each cell during development. The ability of Gro to repress transcription at a distance together with its ability to oligomerize and bind to histones has led to the suggestion that Gro may spread along chromatin. However, much is unknown about the mechanism of Gro-mediated repression and about the dynamics of Gro targeting.ResultsOur chromatin immunoprecipitation sequencing analysis of temporally staged Drosophila embryos shows that Gro binds in a highly dynamic manner primarily to clusters of discrete (

Published
2017

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