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1,146 results on '"Kim, Min-Woo"'

5. Identification of direct connections between the dura and the brain

Author

Smyth, Leon C. D., Xu, Di, Okar, Serhat V., Dykstra, Taitea, Rustenhoven, Justin, Papadopoulos, Zachary, Bhasiin, Kesshni, Kim, Min Woo, Drieu, Antoine, Mamuladze, Tornike, Blackburn, Susan, Gu, Xingxing, Gaitán, María I., Nair, Govind, Storck, Steffen E., Du, Siling, White, Michael A., Bayguinov, Peter, Smirnov, Igor, Dikranian, Krikor, Reich, Daniel S., and Kipnis, Jonathan

Published
2024
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31. Luciferase-LOV BRET enables versatile and specific transcriptional readout of cellular protein-protein interactions

Author

Kim, Christina K, Cho, Kelvin F, Kim, Min Woo, and Ting, Alice Y

Subjects
Biochemistry and Cell Biology, Biological Sciences, Genetics, Generic health relevance, Cytological Techniques, Genes, Reporter, HEK293 Cells, Humans, Light, Luciferases, Molecular Biology, Protein Interaction Mapping, Sensitivity and Specificity, BRET, LOV domain, cell biology, human embryonic kidneys 293 cells, luciferase, protein-protein interactions, Biological sciences, Biomedical and clinical sciences, Health sciences
Abstract

Technologies that convert transient protein-protein interactions (PPIs) into stable expression of a reporter gene are useful for genetic selections, high-throughput screening, and multiplexing with omics technologies. We previously reported SPARK (Kim et al., 2017), a transcription factor that is activated by the coincidence of blue light and a PPI. Here, we report an improved, second-generation SPARK2 that incorporates a luciferase moiety to control the light-sensitive LOV domain. SPARK2 can be temporally gated by either external light or addition of a small-molecule luciferin, which causes luciferase to open LOV via proximity-dependent BRET. Furthermore, the nested 'AND' gate design of SPARK2-in which both protease recruitment to the membrane-anchored transcription factor and LOV domain opening are regulated by the PPI of interest-yields a lower-background system and improved PPI specificity. We apply SPARK2 to high-throughput screening for GPCR agonists and for the detection of trans-cellular contacts, all with versatile transcriptional readout.

Published
2019

35. Alveolar Microdynamics during Tidal Ventilation in Live Animals Imaged by SPring‐8 Synchrotron.

Author

Kim, Min Woo, Yu, Seung Hyeon, Yang, Un, Nukiwa, Ryota, Cho, Hyeon Jung, Kwon, Nam Seop, Yong, Moon Jung, Kim, Nam Ho, Lee, Sang Hyeon, Lee, Jun Ho, Lim, Jae Hong, Kohmura, Yoshiki, Ishikawa, Tatsuya, Henry, Frank S., Imai, Yumiko, Oh, Seung Soo, Hwang, Hyung Ju, Tsuda, Akira, and Je, Jung Ho

Abstract

It is self‐evident that our chests expand and contract during breathing but, surprisingly, exactly how individual alveoli change shape over the respiratory cycle is still a matter of debate. Some argue that all the alveoli expand and contract rhythmically. Others claim that the lung volume change is due to groups of alveoli collapsing and reopening during ventilation. Although this question might seem to be an insignificant detail for healthy individuals, it might be a matter of life and death for patients with compromised lungs. Past analyses were based on static post‐mortem preparations primarily due to technological limitations, and therefore, by definition, incapable of providing dynamic information. In contrast, this study provides the first comprehensive dynamic data on how the shape of the alveoli changes, and, further, provides valuable insights into the optimal lung volume for efficient gas exchange. It is concluded that alveolar micro‐dynamics is nonlinear; and at medium lung volume, alveoli expand more than the ducts. [ABSTRACT FROM AUTHOR]

Published
2024
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37. Mechanisms of Groucho-mediated repression revealed by genome-wide analysis of Groucho binding and activity

Author

Chambers, Michael, Turki-Judeh, Wiam, Kim, Min Woo, Chen, Kenny, Gallaher, Sean D, and Courey, Albert J

Subjects
Biochemistry and Cell Biology, Genetics, Biological Sciences, Human Genome, Biotechnology, 2.1 Biological and endogenous factors, Aetiology, 1.1 Normal biological development and functioning, Underpinning research, Generic health relevance, Animals, Basic Helix-Loop-Helix Transcription Factors, Chromatin, Drosophila melanogaster, Embryo, Nonmammalian, Gene Expression Regulation, Developmental, Genomics, Protein Binding, Repressor Proteins, Groucho, Transcriptional repression, Drosophila embryogenesis, ChIP-seq, RNA-seq, Chromatin-associated RNA-seq, RNA polymerase II pausing, Chromatin-associated RNA-seq, RNA polymerase II pausing, Information and Computing Sciences, Medical and Health Sciences, Bioinformatics, Biological sciences, Biomedical and clinical sciences
Abstract

BackgroundThe transcriptional corepressor Groucho (Gro) is required for the function of many developmentally regulated DNA binding repressors, thus helping to define the gene expression profile of each cell during development. The ability of Gro to repress transcription at a distance together with its ability to oligomerize and bind to histones has led to the suggestion that Gro may spread along chromatin. However, much is unknown about the mechanism of Gro-mediated repression and about the dynamics of Gro targeting.ResultsOur chromatin immunoprecipitation sequencing analysis of temporally staged Drosophila embryos shows that Gro binds in a highly dynamic manner primarily to clusters of discrete (

Published
2017

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