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141 results on '"Kim, Shin‐Kwon"'

4. Stable Production of a Recombinant Single-Chain Eel Follicle-Stimulating Hormone Analog in CHO DG44 Cells.

Author

Byambaragchaa, Munkhzaya, Park, Sei Hyen, Kim, Sang-Gwon, Shin, Min Gyu, Kim, Shin-Kwon, Park, Myung-Hum, Kang, Myung-Hwa, and Min, Kwan-Sik

Subjects
FOLLICLE-stimulating hormone, EELS, PEPTIDES, CHO cell, MOLECULAR weights, AMINO acids
Abstract

This study aimed to produce single-chain recombinant Anguillid eel follicle-stimulating hormone (rec-eel FSH) analogs with high activity in Cricetulus griseus ovary DG44 (CHO DG44) cells. We recently reported that an O-linked glycosylated carboxyl-terminal peptide (CTP) of the equine chorionic gonadotropin (eCG) β-subunit contributes to high activity and time-dependent secretion in mammalian cells. We constructed a mutant (FSH-M), in which a linker including the eCG β-subunit CTP region (amino acids 115–149) was inserted between the β-subunit and α-subunit of wild-type single-chain eel FSH (FSH-wt). Plasmids containing eel FSH-wt and eel FSH-M were transfected into CHO DG44 cells, and single cells expressing each protein were isolated from 10 and 7 clones. Secretion increased gradually during the cultivation period and peaked at 4000–5000 ng/mL on day 9. The molecular weight of eel FSH-wt was 34–40 kDa, whereas that of eel FSH-M increased substantially, with two bands at 39–46 kDa. Treatment with PNGase F to remove the N glycosylation sites decreased the molecular weight remarkably to approximately 8 kDa. The EC50 value and maximal responsiveness of eel FSH-M were approximately 1.23- and 1.06-fold higher than those of eel FSH-wt, indicating that the mutant showed slightly higher biological activity. Phosphorylated extracellular-regulated kinase (pERK1/2) activation exhibited a sharp peak at 5 min, followed by a rapid decline. These findings indicate that the new rec-eel FSH molecule with the eCG β-subunit CTP linker shows potent activity and could be produced in massive quantities using the stable CHO DG44 cell system. [ABSTRACT FROM AUTHOR]

Published
2024
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8. Antiseptic religion : missionary medicine in 1885-1910 Korea

Author

Kim, Shin Kwon and Harrison, Mark

Subjects
610.6909519, Missionary Medicine, History of Medicine and Religion, East Asia and Korea, Religion, History of Medicine, Protestant Mission, Epidemic Disease
Abstract

The thesis explores the intersection between medicine and religion in the context of colonisation in Korea in the late nineteenth and early twentieth century. I will focus on the work of medical missionaries from Europe and North America that pursued perfect cleanliness in body, mind and society, including total abstinence and spiritual cleanliness, by spreading biomedical concept of hygiene. One of the points that I will articulate is the ways in which medicine as a colonising force in its own right worked in the mission field to produce 'the docile bodies of people' in the Foucauldian sense. I will argue that what mission medicine in Korea utilised and relied on for its work was a new concept of cleanliness based on biomedical knowledge, the germ theory, rather than the power of colonisation. It was because mission medicine in Korea often worked without collaborating with direct colonial powers. In this sense, Protestant Christianity and biomedicine shared a common foundation in 'cleanliness.' Consequently, I will try to emphasise the multi-dimensional and multi-directional role of the use of cleanliness as an efficacious tool for control of the body. In relation to the historiography of medicine in Korea, I will argue that Confucianism served the social and cultural control of bodies as a medicalised form and that Christianity tried to replace it by providing new knowledge concerning body, disease, health, and cleanliness. In the same respect, I will explore the historical relationship between the germ theory and missionary medicine in Korea. The germ theories of disease were not simply a new etiology but also an effective cultural implement to change people's lives. Thus, the theories did not simply remain in the realm of medicine but were introduced, disseminated, and applied to all matters relating to the body, including its mental and spiritual aspects, through the concept of cleanliness.

Published
2017

11. Effect of Host-Associated Bacillus-Supplemented Artificial Diets on Growth, Survival Rate, and Gene Expression in Early-Stage Eel Larvae (Anguilla japonica)

Author

Jang, Won Je, primary, Kim, Shin-Kwon, additional, Park, So Young, additional, Kim, Dong Pil, additional, Heo, Yun-Jy, additional, Kim, Haham, additional, Lee, Su-Jeong, additional, Shin, Min Gyu, additional, Lee, Eun-Woo, additional, Lee, Seunghyung, additional, and Lee, Jong Min, additional

Published
2023
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15. DNA barcoding and morphological identification of spiny lobsters in South Korean waters: a new record of Panulirus longipes and Panulirus homarus homarus

Author

Hettiarachchi, Sachithra Amarin, primary, Hyeon, Ji-Yeon, additional, Mahardini, Angka, additional, Kim, Hyung-Suk, additional, Byun, Jun-Hwan, additional, Kim, Han-Jun, additional, Jeong, Jong-Gyun, additional, Yeo, Jung-Kyu, additional, Kim, Shin-Kwon, additional, Kim, Se-Jae, additional, Heo, Youn-Seong, additional, Sathyadith, Jonathan, additional, Kang, Do-Hyung, additional, and Hur, Sung-Pyo, additional

Published
2022
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18. Characterization of Latilactobacillus curvatus MS2 isolated from Korean traditional fermented seafood and cholesterol reduction effect as synbiotics with isomalto-oligosaccharide in BALB/c mice

Author

Kim, Shin-Kwon, primary, Jang, Won Je, additional, Kim, Chae Eun, additional, Lee, Su-Jeong, additional, Jeon, Mi-Hyeon, additional, Kim, Tae-Yong, additional, Lee, Hyun-Tai, additional, Park, Jung-Ha, additional, Kim, Chan-Hee, additional, Lee, Jong Min, additional, and Lee, Eun-Woo, additional

Published
2021
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20. Identification and molecular characterization of peroxiredoxin 6 from Japanese eel (Anguilla japonica) revealing its potent antioxidant properties and putative immune relevancy

Author

Priyathilaka, Thanthrige Thiunuwan, Kim, Yucheol, Udayantha, H. M.V., Lee, Seongdo, Herath, H. M.L.P.B., Lakmal, Chaminda H.H., Elvitigala, Don Anushka Sandaruwan, Umasuthan, Navaneethaiyer, Godahewa, G. I., Kang, Seong Il, Jeong, Hyung Bok, Kim, Shin Kwon, Kim, Dae Jung, and Lim, Bong Soo

Published
2016
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36. Addition of the red macroalgae Turkish Towel Chondracanthus exasperates and taurine improves the performance of alternative plant‐based feeds for juvenile sablefish Anoplopoma fimbria

Author

Johnson, Ronald B., primary, Nicklason, Peter M., additional, Armbruster, Lisa C., additional, Sommers, Frank C., additional, Kim, Shin‐Kwon, additional, Marancik, David, additional, Jee, Junghoon, additional, Gadberry, Brad A., additional, and Colt, John E., additional

Published
2020
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42. Effects of Lactococcuslactis subsp. lactis I2 with ��-Glucooligosaccharides on Growth, Innate Immunity and Streptococcosis Resistance in Olive Flounder (Paralichthys olivaceus)

Author

Hasan, Tawheed, primary, Jang, Won Je, additional, Tak, Jin Yeong, additional, Lee, Bong-Joo, additional, Kim, Kang Woong, additional, Hur, Sang Woo, additional, Han, Hyon-Sob, additional, Kim, Bo-Seong, additional, Min, Do-Huh, additional, Kim, Shin-Kwon, additional, and Kong, In-Soo, additional

Published
2018
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44. Digestive Physiological Characteristics of the Gobiidae

Author

Hur, Sang-Woo, primary, Kim, Shin-Kwon, additional, Kim, Dae-Jung, additional, Lee, Bae-Ik, additional, Park, Su-Jin, additional, Hwang, Hyung-Gyu, additional, Jun, Je-Cheon, additional, Myeong, Jeong-In, additional, Lee, Chi-Hoon, additional, and Lee, Young-Don, additional

Published
2016
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