1. A physical map of the mouse genome
- Author
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Asif T. Chinwalla, Daniel A. Russell, Richard D. Hutton, Rebecca McGrane, Pawan Pandoh, Catharine Gray, Margaret Krol, Kazutoyo Osoegawa, Mandeep Sekhon, Ewan Birney, Marco A. Marra, Michael Heaney, Reta Kutsche, R Evans, Pieter J. de Jong, Soo Sen Lee, Tony Cox, Elizabeth Gebregeorgis, Carol Scott, Jacqueline E. Schein, Duane E Smailus, David R. Bentley, Parvaneh Saeedi, Glen Threadgold, Ian R Mullenger, Suganthi Chittaranjan, Chris Fjell, Robert H. Waterston, Jim Stalker, Letticia Hsiao, Jason Maas, Martin Krzywinski, Jason Carter, John Douglas Mcpherson, Kelly Mead, Simon G. Gregory, Ian Bosdet, Bola Ayodeji, Anna-Liisa Prabhu, Joel A. Malek, George S. Yang, Dan Layman, Tony Gaige, Keita Geer, Jane Rogers, Tamara Feldblyum, Miranda Tsai, Larry Overton, Sara Jaeger, LaDeana W. Hillier, Kristine M. Wylie, Colin Kremitzki, Sheryl Taylor, Carrie Mathewson, Jyoti Shetty, Wesley Terpstra, James Smith, Getahun Tsegaye, Christopher A Fox, Steve Messervier, Natasja Wye, Candice McLeavy, Jill Vardy, William C. Nierman, Lorraine Spence, Alla Shvartsbeyn, Sofiya Shatsman, Readman Chiu, Claire M. Fraser, Michael Smith, John W. Wallis, Michael C. Holmes, Tim Hubbard, Ran Guin, Shaying Zhao, Jeffrey L Stott, Noreen Girn, Kimbly J Phillips, Rebecca S. Walker, Paul W. Burridge, Joseph J. Catanese, Steven J.M. Jones, Steven R. Ness, Dan Fuhrmann, Susanna Chan, and Jennifer Asano
- Subjects
Molecular Sequence Data ,Sequence alignment ,Computational biology ,Biology ,Genome ,Synteny ,Contig Mapping ,Chromosomes ,Mice ,Species Specificity ,Sequence Homology, Nucleic Acid ,Animals ,Humans ,Cloning, Molecular ,Conserved Sequence ,Genetics ,Radiation Hybrid Mapping ,Multidisciplinary ,Contig ,Genome, Human ,Genome project ,Physical Chromosome Mapping ,Human genome ,Chromosomes, Human, Pair 6 ,Sequence Alignment ,Reference genome - Abstract
A physical map of a genome is an essential guide for navigation, allowing the location of any gene or other landmark in the chromosomal DNA. We have constructed a physical map of the mouse genome that contains 296 contigs of overlapping bacterial clones and 16,992 unique markers. The mouse contigs were aligned to the human genome sequence on the basis of 51,486 homology matches, thus enabling use of the conserved synteny (correspondence between chromosome blocks) of the two genomes to accelerate construction of the mouse map. The map provides a framework for assembly of whole-genome shotgun sequence data, and a tile path of clones for generation of the reference sequence. Definition of the human-mouse alignment at this level of resolution enables identification of a mouse clone that corresponds to almost any position in the human genome. The human sequence may be used to facilitate construction of other mammalian genome maps using the same strategy.
- Published
- 2002