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Background: As the pet population grows, there is increasing attention on the health and well-being of companion animals. Weaning, a common challenge for young mammals, often leads to issues such as diarrhea, growth retardation, and in severe cases, even mortality. However, the specific changes in gut microbiota and metabolites in kittens following weaning remain unclear. In this study, we conducted a comprehensive investigation of the dynamic changes in the gut microbiota, serum metabolism, antioxidant capacity, and immune function of kittens at various time points: days 0, 4, and 30 post-weaning. Results: Significant changes in the immune response and gut microbiota were observed in kittens following weaning. Specifically, IgM levels increased significantly (P < 0.01, n = 20), while IgA and IgG levels showed a sustained elevation. Weaning also disrupted the intestinal microbiota, leading to notable changes in serum metabolism. On day 4 post-weaning, there was a decrease in beneficial bacteria such as Bacteroides vulgatus, Fusobacterium nucleatum, Anaerostipes caccae, and Butyricico-ccaceae. However, by day 30, beneficial bacteria including Candidatus Arthro-mitus, Holdemanella, and Bifidobacterium had increased (P < 0.05, n = 20). Serum metabolites showed clear separation across time points, with day 0 and day 4 exhibiting similar patterns. A total of 45 significantly altered metabolites (P < 0.05, n = 20) were identified, primarily related to vitamins, steroids, peptides, organic acids, lipids, and carbohydrates. Pathway analysis revealed significant enrichment in eight metabolic pathways, with key changes in arginine metabolism and biosynthesis. Additionally, bacteria such as Bacteroides fragilis, Bacteroides stercoris, Leuconostoc citreum, and Bifidobacterium adolescentis were positively correlated with serum metabolic changes, emphasizing the link between gut microbiota and systemic metabolism (P < 0.05, n = 20). Conclusion: Our study demonstrated that the composition and function of intestinal microorganisms as well as serum metabolic profiles of weaned kittens presented dynamic changes. These findings not only deepen our understanding of the effects of weaning on kitten health, but also provide valuable insights into post-weaning nutritional regulation strategies for kittens. [ABSTRACT FROM AUTHOR]

Simple Summary: The juvenile period is a critical stage of cat growth; in particular, the metabolic activity of the kitten's intestinal microbiota is critical to overall health. This study investigated the effects of a probiotic complex on the intestinal health of kittens. We found that complex probiotics significantly altered the levels of metabolites associated with inflammation and constipation in the intestines of kittens. This suggests that complex probiotics may promote intestinal health in kittens by modulating changes in various metabolites. This study provides a rationale for the development of probiotic supplements for kittens. Cats are popular companions for humans, and their health is of importance to a growing number of pet owners. The juvenile period is a critical stage of cat growth; in particular, the metabolic activity of the intestinal microbiome of kittens is critical for processing nutrients and supporting overall health. In this study, the effect of complex probiotics on the intestinal health of kittens was explored through a metabolomics analysis. Twenty-four healthy kittens were randomly assigned to two groups (n = 12): the control group was provided a basal diet and the probiotics group was provided the basal diet supplemented with complex probiotics (given at the same time daily). The kittens were acclimatized for 5 days, and the experiment was conducted for 14 days. We collected feces from each kitten on days 1 and 14 for metabolomic analyses. Compared to the control, the probiotics group had significantly higher (p < 0.05) methylmalonylcarnitine, lysyl-hydroxyproline, phenylpropionylglycine, and vitamin K3 levels, and significantly lower (p < 0.05) gamma-glutamyl-L-putrescine, cis-gondoic acid, myristic acid, 12,13-DiHOME, and glycodeoxycholic acid levels. The results of this study suggest that complex probiotics promote intestinal health in kittens by regulating changes in various metabolites in the intestine and may have a mitigating effect on intestinal inflammation. [ABSTRACT FROM AUTHOR]

Abstract Background As the pet population grows, there is increasing attention on the health and well-being of companion animals. Weaning, a common challenge for young mammals, often leads to issues such as diarrhea, growth retardation, and in severe cases, even mortality. However, the specific changes in gut microbiota and metabolites in kittens following weaning remain unclear. In this study, we conducted a comprehensive investigation of the dynamic changes in the gut microbiota, serum metabolism, antioxidant capacity, and immune function of kittens at various time points: days 0, 4, and 30 post-weaning. Results Significant changes in the immune response and gut microbiota were observed in kittens following weaning. Specifically, IgM levels increased significantly (P

Identifying when kittens are in fearful emotional states is crucial to protecting welfare, preventing behavioral issues, and ensuring caretaker and researcher assessments are accurate. Research is needed to confirm if kittens have fully developed fear responses similar to those of adult cats when they are in situations that evoke fear. To assess which behaviors kittens show when they are avoiding novel and startling stimuli, kittens (5 to 8 weeks old; n = 46) completed trials involving exposure to such stimuli, alternating with blank control trials. Trials were separated into immediate responses (during a short stimulus presentation or equivalent control period), and delayed responses (after stimulus removal). Mixed regression models, with litter and kitten as random intercepts, were used to model durations and frequencies of behaviors. Across both time phases, kittens displayed significantly longer durations of arched back, piloerection, freezing, and tail tucking, shorter durations of eating, and greater rates of putting their ears back during fear trials compared to blank trials. During stimulus presentations, kittens displayed significantly shorter durations of retreating to a hutch and greater rates of flinching, whereas after stimulus removal, kittens displayed significantly longer durations of crouching and shorter durations of upright tail. Responses were also affected by kitten coat color, sex, and being mother-reared. Thus, fear behaviors in 5-to-8-week-old kittens are fully developed and similar to what is observed in adult cats. These results allow for accurate identification of fear in kittens, with the goal of improving research on kitten fear development and kitten welfare.

Copyright of Summa, Animali da Compagnia is the property of Point Veterinaire Italie s.r.l. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

In the feline species, the mortality of live-born kittens over the first 2 months of life is around 10%. Although some losses are inevitable, progress in veterinary medicine and improved knowledge of feline neonatalogy should make it possible to reduce them to a lower level. The objectives of this study were: (i) to describe early growth in kittens, and (ii) to assess whether low birth weight kittens develop compensatory growth during the first week of life and if so, whether compensatory growth is associated with increased chances of survival. Using data collected from 5,504 kittens born in 193 different French catteries, five growth rates were calculated to reflect the growth of kittens during the first week after birth. Low birth weight kittens had higher growth rates than normal birth weight kittens. In addition, low birth weight kittens whose early growth was in the lowest 25% had a significantly higher 0-2 months mortality rate than all the other groups. Weight loss (or lack of weight gain) between birth and Day 2 was identified as a risk factor for 0-2 months mortality whatever the birth weight category. Finally, critical early growth thresholds were determined separately for low and normal birth weight kittens. These figures could help caregivers to validate the adequacy or inadequacy of kitten early growth. They will be able to quickly identify and provide appropriate care for the kittens whose growth is deemed insufficient in order to improve their chances of survival. [ABSTRACT FROM AUTHOR]

Case summary Two 18-day-old domestic shorthair kittens presented with dyspnoea, mild cyanosis, heart murmur, lung pattern, abnormal crawling and a marked dorsoventral flattening of the thorax. Deformity of the thoracic wall without pectus excavatum was diagnosed. Cardboard corsets tailored to each kitten were applied. This gave immediate respiratory relief, easing distress and pain. After 10 days, clinical and radiographic monitoring revealed normalisation of the shape of the chest wall with an improvement in general condition. At 6 months of age, clinical examination showed normal growth and development of both kittens, with a normal thoracic profile and shape also seen on radiographs. Relevance and novel information Data about flat-chested kitten syndrome and its aetiopathogenesis, treatment and outcome are scarce, but there is significant experience among breeders that is shared through online communities. High mortality rates are reported. The use of a cardboard splint appears to be novel in the published literature; in this case report, it proved to be a rapid and easy solution. For this reason, cardboard splints could be considered as a first approach for the clinical management of flat-chested kitten syndrome. [ABSTRACT FROM AUTHOR]

Extended maternal care, defined by protection, nursing and training of young, is a hallmark of mammalian reproduction. Prior maternal experience and maternal caregiving decisions during rearing may influence the success of offspring. Because most maternal caregiving behaviour, such as nursing and defence, requires contact or close proximity to the young, time spent with offspring may be a good proxy for maternal care provision when direct maternal behaviour is unobservable. Although different behavioural patterns can influence how carnivore mothers balance the competing energetic costs of brood raising and food acquisition, relatively little is known about the allocation of maternal care to neonates in solitary felids, such as cougars, Puma concolor. To evaluate how maternal behaviour and provisioning affect kitten survival, we remotely monitored 16 female cougars and their 49 neonate kittens using proximity-linked VHF collars on neonates and paired GPS collars on adults. We deployed collars on 5.5-week-old kittens and monitored their survival to 180 days postbirth. We derived several metrics to quantify maternal care behaviours and fitted Bayesian known-fate survival models to estimate the daily survival probability of kittens and to evaluate the influence of maternal behaviour on kitten survival. We found that kittens who received increased levels of maternal care, such as more time with their mother each day, had higher survival rates among both first-time and experienced mothers. However, experienced mothers exhibited higher kitten survival across all scenarios compared to first-time mothers. Our results suggests that prior maternal experience is a strong driver of increased kitten survival rates and that kitten survival rates are increased when mothers provide more maternal care. • For many mammals, maternal caregiving can be influential to survival of young. • Maternal care or prior experience may lead to better kitten survival outcomes. • rVHF collars on neonates can provide information about proximity to the mother. • Cougar kitten survival was higher from mothers who had previously raised kittens. • Maternal caregiving increased kitten survival for first-time and experienced moms. [ABSTRACT FROM AUTHOR]

Introduction: The domestic cat (Felis catus) is one of the most common pets. Worldwide, approximately one in five adults are sensitive to cat allergens. The major cat allergen is the secretoglobulin Fel d 1, which is primarily produced in the salivary and sebaceous glands. Chickens produce IgY antibodies, which are similar in structure to mammalian IgG. When chickens are exposed to Fel d 1, anti-Fel d 1-specific IgY (AFD1) is produced and is naturally concentrated in egg yolk. The aim of this study was to evaluate the tolerability, effects on growth and food consumption, and potential adverse effects of a chicken egg product ingredient containing AFD1 in kittens. Methods: This was a blinded, controlled study. Twenty-seven (27) eight-week old kittens were randomly assigned to three feeding groups containing 0 ppm AFD1 (Group 0), 8 ppm AFD1 (Group 1), and 16 ppm AFD1 (Group 2) for 84 days. Veterinary exams and bloodwork were performed on Day 42 and Day 84, and body weight and body condition score (BCS) were monitored weekly. Results: Throughout the study, there were no signs of nutritional deficiency or adverse clinical events in any of the subjects. Administration of a chicken egg product ingredient containing AFD1 in the diet (whether in coating or combination of coating and top dress) had no significant effect on body weight nor food consumption, and all subjects maintained a healthy Body Condition Score (BCS) throughout the study. Moreover, there were no biologically significant differences in the mean clinical chemistry and hematology parameters. Discussion: This study demonstrated that a diet formulated to contain up to 16 ppm AFD1, included in the coating and the top-dress of dry kitten food, was well tolerated, promoted adequate growth, and exhibited no adverse effects. [ABSTRACT FROM AUTHOR]

In the feline species, the mortality of live-born kittens over the first 2 months of life is around 10%. Although some losses are inevitable, progress in veterinary medicine and improved knowledge of feline neonatalogy should make it possible to reduce them to a lower level. The objectives of this study were: (i) to describe early growth in kittens, and (ii) to assess whether low birth weight kittens develop compensatory growth during the first week of life and if so, whether compensatory growth is associated with increased chances of survival. Using data collected from 5,504 kittens born in 193 different French catteries, five growth rates were calculated to reflect the growth of kittens during the first week after birth. Low birth weight kittens had higher growth rates than normal birth weight kittens. In addition, low birth weight kittens whose early growth was in the lowest 25% had a significantly higher 0–2 months mortality rate than all the other groups. Weight loss (or lack of weight gain) between birth and Day 2 was identified as a risk factor for 0–2 months mortality whatever the birth weight category. Finally, critical early growth thresholds were determined separately for low and normal birth weight kittens. These figures could help caregivers to validate the adequacy or inadequacy of kitten early growth. They will be able to quickly identify and provide appropriate care for the kittens whose growth is deemed insufficient in order to improve their chances of survival.

A two-month-old kitten was presented to TVCC, Mannuthy with dyspnoea and open mouth breathing. Auscultation of the thorax revealed muffled heart sounds. Radiography showed loss of normal diaphragmatic border, obscured cardiac silhouette and displaced gas filled intestinal loops into the thoracic cavity which were suggestive of diaphragmatic hernia. Under inhalant anaesthesia with intermittent positive pressure ventilation, was performed by cranial mid ventral celiotomy from umbilicus to the costo chondral arch. Lobes of liver along with intestinal loops were repositioned into abdominal cavity through diaphragmatic defect and the defect was successfully closed. Abdominal wall and skin were apposed in regular manner. The animal recovered uneventfully. [ABSTRACT FROM AUTHOR]

Simple Summary: Newborns are susceptible to infectious disease, and early life represents a critical developmental window of the immune system, which is important for preventing and fighting disease. There is evidence that nucleotides, oligosaccharides and vitamins can positively influence immune function; however, the evidence that is specifically applicable to cats is limited. This current study fed domestic short hair kittens either a dry format control diet or a test diet fortified with nucleotides, short-chain fructooligosaccharides, xylooligosaccharides, β-carotene and vitamin E until the kittens reached 28 weeks of age. The kittens followed a routine preventative medication schedule for vaccination and a suite of health, metabolism and immune parameters were measured at regular intervals throughout the study. All kittens remained healthy and did not display any clinically relevant signs of adverse health. Antibodies are produced following infection or vaccination. The level of antibody, the proportion of kittens who demonstrated an acceptable response to vaccination and the proportion of kittens who reached the recognised protective level of antibody were greater in kittens fed the test diet. The test diet group demonstrated a stronger antibody-mediated response to vaccination. Antibodies play a role in preventing disease; thus, this suggests that the test diet supports immune defence against immune challenges. Nucleotides, short-chain fructooligosaccharides (scFOS), xylooligosaccharides (XOS), β-carotene and vitamin E are reported to enhance immune function; however, the evidence of this in cats is limited. The aim of this study was to determine the immunomodulatory effects of these ingredients in kittens. Forty domestic short hair kittens were designated in litters to control or test diet for 28 weeks. Test diet was fortified with 0.33 g nucleotides, 0.45 g scFOS, 0.3 g XOS, 0.7 mg β-carotene and 66.5 mg vitamin E per 100 g diet. Kittens were vaccinated against feline parvovirus (FPV) and herpesvirus (FHV) at 10, 14 and 18 weeks. Kittens remained healthy, with no measured evidence of adverse health. Serum FPV and FHV antibody titres were significantly (p < 0.05) higher in the test diet group at week 23 and 27, respectively. A significantly (p < 0.05) higher proportion of test diet group kittens demonstrated an adequate response (four-fold titre increase) to FHV vaccination and a significantly (p < 0.05) higher proportion reached a protective antibody titre for FHV. Serum IgM was significantly (p < 0.05) higher in the test diet group. The test diet group demonstrated a stronger humoral immune response to vaccination, suggesting the diet supports immune defence, enabling a greater response to immune challenges. [ABSTRACT FROM AUTHOR]

Objectives: The aim of the present study was to investigate the construct validity, responsiveness and reliability of the Feline Grimace Scale (FGS) in kittens. Methods: A total of 36 healthy female kittens (aged 10 weeks to 6 months) were included in a prospective, randomized, blinded study. Video recordings of all kittens were made before and 1 and 2 h after ovariohysterectomy using an opioid-free injectable anesthetic protocol with or without multimodal analgesia. Additional recordings were taken before and 1 h after administration of rescue analgesia (buprenorphine 0.02 mg/kg IM) to painful kittens. Screenshots of facial images were collected from the video recordings for FGS scoring. Four observers blinded to treatment groups and time points scored 111 randomized images twice with a 5-week interval using the FGS. Five action units (AUs) were scored (ear position, orbital tightening, muzzle tension, whiskers position and head position; 0–2 each). Construct validity, responsiveness, and inter- and intra-rater reliability were evaluated using linear models with Benjamini–Hochberg correction, Wilcoxon signed-rank test and single intraclass correlation coefficients (ICCsingle), respectively (P <0.05). Results: FGS total ratio scores were higher at 1 and 2 h after ovariohysterectomy (median [interquartile range, IQR]: 0.30 [0.20–0.40] and 0.30 [0.20–0.40], respectively) than at baseline (median [IQR]: 0.10 [0.00–0.30]) (P <0.001). FGS total ratio scores were lower after the administration of rescue analgesia (median [IQR] before and after rescue analgesia) 0.40 [0.20–0.50] and 0.20 [0.10–0.38], respectively (P <0.001). Inter-rater ICCsingle was 0.68 for the FGS total ratio scores and 0.35–0.70 for all AUs considered individually. Intra-rater ICCsingle was 0.77–0.91 for the FGS total ratio scores and 0.55–1.00 for all AUs considered individually. Conclusions and relevance: The FGS is a valid and responsive acute pain-scoring instrument with moderate inter-rater reliability and good to excellent intra-rater reliability in kittens. [ABSTRACT FROM AUTHOR]

Copyright of Kocatepe Veterinary Journal / Kocatepe Veteriner Dergisi is the property of Afyon Kocatepe University, Faculty of Veterinary Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

A 3-month-old kitten weighing 1.05 kg was presented to the University veterinary hospital at Kokkalai in Thrissur district of Kerala, India, with the complaint of open-mouth breathing, lethargy and complete anorexia for the past three days, and weight loss. Detailed clinical examination revealed inspiratory dyspnoea without stridor, tachypnoea, muffled lung and heart sounds on auscultation, pale mucous membrane, and weak pulse. Haematology revealed leukocytosis with granulocytosis and monocytosis. Thoracic radiography, ultrasonography and thoracocentesis confirmed pyothorax. Microbial culture of thoracic aspirate revealed heavy growth of Acinetobacte spp. The cat responded to parenteral therapy with ceftiofur followed by cefixime orally for 21 days. Doxycycline was prescribed for concurrent mycoplasmosis. Thoracic radiograph 10 days later revealed a significant reduction in pleural fluid. A review of the case two months later found that the kitten recovered uneventfully. A rare case of Acinetobacter spp. associated pyothorax and its medical management in a cat is reported. The clinical manifestations, radiographic changes and ultrasonographic findings were discussed.

This study aimed to examine the distribution of feline calicivirus (FCV), feline herpesvirus (FHV), and feline panleukopenia virus (FPLV), which cause neonatal feline viral diseases in kittens aged one to three months, using a Dot-ELISA bases antibody test kit. The studied parameters included the animals" sex, clinical signs and the Dot-ELISA test kit values. Twenty kittens had eye lesions and 20 were without eye lesions. Basic ophthalmologic examinations were performed, including pupil, corneal, palpebral and menace response reflexes, direct ophthalmoscopy, and fluorescein staining. The study population consisted of 40 kittens (25 female and 15 male); 3 of them are British shorthaired and the rest is 37 tabby kittens. In half of the 20 kittens with eye lesions, the lesions were bilateral and the most common clinical lesions were conjunctivitis, mucopurulent discharge, and blepharospasm. Other notable clinical findings were iris staphyloma, corneal opacity, symblepharon, and panophthalmitis. A higher rate of seropositive results was determined against Calicivirus in kittens. The severity and appearance of the cases could vary depending on the virus accompanying the lesions. In conclusion, the Feline Calicivirus was the most frequently detected virus in 1 to 3-month-old kittens in this study and the clinical presentation may change according to the accompanying virus titers.

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Simple Summary: Low birth weight has been shown to increase the risk of mortality in feline species. Thus, it is important to provide pet professionals with weight thresholds in order to enable them to make the best use of this simple and inexpensive, but essential, management practice, i.e., weighing kittens at birth. Based on data collected from 194 French catteries, this study defined birth weight thresholds which allow for the identification of kittens at higher risk of 0–2 months mortality in 15 breeds (5596 kittens). Two thresholds were identified, classifying kittens into three groups: normal, low, and very low birth weight, characterized by low, moderate, and high risk of 0–2 months mortality, respectively. Values defining very low birth weight kittens varied between 60 g and 78 g depending on the breed and the values defining low birth weight kittens were between 74 g and 104 g. When used as alarm thresholds, these values will facilitate the detection of kittens requiring specific nursing. In many species, low birth weight is identified as a major determinant for neonatal survival. The objectives of the present study were (i) to assess, in a large feline purebred population, the impact of birth weight on 0–2 months mortality in kittens, and (ii) if such mortality occurs, to define cut-off values for birth weight to identify at-risk kittens. Data from 5596 kittens from 15 breeds and provided by 194 French breeders were analysed. A logistic mixed model was used to identify low birth weight, being a male, and being born in a large litter as significant risk factors for kitten mortality during the first two months after birth. Classification and regression tree analysis was used to define the thresholds, first at the species level and, when possible, at the breed level. Two thresholds were defined to group kittens into three categories: low, moderate, or high risk of 0–2 months mortality (normal, low, and very low birth weight, respectively). In our population, 19.7% of the kittens were classified as low birth weight and 1.9% as very low birth weight. Critical thresholds may differ between breeds with similar birth weight distributions and equivalent mortality rates (e.g., Russian Blue/Nebelung vs. Egyptian Mau). These critical birth weight thresholds, established in 15 breeds, could be used to identify kittens requiring more intensive nursing to improve survival. [ABSTRACT FROM AUTHOR]

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Kittens are prone to intestinal health problems as their intestines are not completely developed. Seaweed is rich in plant polysaccharides and bioactive substances that are highly beneficial to gut health. However, the effects of seaweed on cat gut health have not been assessed. This study compared the effects of dietary supplementation with enzymolysis seaweed powder and Saccharomyces boulardii on the intestinal health of kittens. In total, 30 Ragdoll kittens (age: 6 months; weight: 1.50 ± 0.29 kg) were assigned to three treatment groups for a 4-week feeding trial. The dietary treatment given was as follows: (1) basal diet (CON); (2) CON + enzymolysis seaweed powder (20 g/kg of feed) mixed evenly with the diet (SE); and (3) CON + Saccharomyces boulardii (2 × 1010 CFU/kg of feed) mixed evenly with the diet (SB). Compared with the CON and SB groups, dietary supplementation with the enzymolysis seaweed powder improved the immune and antioxidant capacity and also reduced the intestinal permeability and inflammation levels of kittens. The relative abundance of Bacteroidetes, Lachnospiraceae, Prevotellaceae, and Faecalibacterium in the SE group was higher than those in the CON and SB groups (p ≤ 0.05), while the relative abundance of Desulfobacterota, Sutterellaceae, and Erysipelatoclostridium in the SB group was lower than that in the SE group (p ≤ 0.05). Moreover, enzymolysis seaweed powder did not alter the level of intestinal SCFAs in kittens. Conclusively, supplementing kitten diet with enzymolysis seaweed powder can promote intestinal health by enhancing the gut barrier function and optimizing the microbiota composition. Our findings provide new perspectives on the application of enzymolysis seaweed powder. [ABSTRACT FROM AUTHOR]

A two-month-old, male intact, mixed-breed cat weighing 0.6 kg was presented with respiratory distress and anorexia. From the transthoracic echocardiographic, reduced fractional shortening (FS) and increased endocardial echogenicity were recognised with severe congestive heart failure (CHF). The kitten was administered an antibiotic and pimobendane under oxygen supplementation in an ICU cage. However, the respiratory condition worsened and the cat died the next day, and the subsequent necropsy and histopathology examinations confirmed endocardial fibroelastosis (EFE). There is a lack of information regarding the antemortem cardiac function evaluated by tissue Doppler imaging (TDI) in EFE cases. We report on the echocardiographic findings including the TDI in the EFE cat with a concomitant necropsy and histopathology confirmation in this paper. The echocardiographic findings showed presence of a ventricular false tendon within the left ventricle, a decrease in the left ventricular contractility (FS 11.1%, and a marked CHF). In this case, the echocardiographic findings were consistent with the human counterpart. However, these findings were like those of dilated cardiomyopathy and, hence, non-specific to EFE. As a result, veterinarians should keep in mind that endocardial fibroelastosis might be a possible reason for respiratory distress resulting from CHF with a low fractional shortening in young cats.

Kitten is the earliest stage of a cat’s life, starting from 0 – 6 months old. As the golden age of a human baby in their first 1000 days of life, a kitten is the golden period for cats to grow and explore the environments. People tend to adopt cats at this stage as it is easier for them to shape and understand the characteristics of their cats. Therefore, understanding the behaviors of cats during the kitten period is fundamental. This study observed the behaviors of two pet kittens at 13 weeks of age when they roamed around the urban backyard. An ethogram was designed to record the duration of states (in second) and the frequency of events (per observation) of all behaviors. During the assessments, both kittens dominantly performed exploratory behaviors. They tended to explore the environment by sniffing ground, climbing trees, staring at birds, and walking around the backyard. These behaviors could be seen more often than their playing behaviors (object play and social play). In conclusion, this study supports the view that during the late socialization period (9-16 weeks), kittens seem to show the intense exploration of the environment as part of the development of predatory behavior or hunting skills in wild nature settings.

A 3-month-old kitten weighing 1.05 kg was presented to the University veterinary hospital at Kokkalai in Thrissur district of Kerala, India, with the complaint of open-mouth breathing, lethargy and complete anorexia for the past three days, and weight loss. Detailed clinical examination revealed inspiratory dyspnoea without stridor, tachypnoea, muffled lung and heart sounds on auscultation, pale mucous membrane, and weak pulse. Haematology revealed leukocytosis with granulocytosis and monocytosis. Thoracic radiography, ultrasonography and thoracocentesis confirmed pyothorax. Microbial culture of thoracic aspirate revealed heavy growth of Acinetobacter spp. The cat responded to parenteral therapy with ceftiofur followed by cefixime orally for 21 days. Doxycycline was prescribed for concurrent mycoplasmosis. Thoracic radiograph 10 days later revealed a significant reduction in pleural fluid. A review of the case two months later found that the kitten recovered uneventfully. A rare case of Acinetobacter spp. associated pyothorax and its medical management in a cat is reported. The clinical manifestations, radiographic changes and ultrasonographic findings were discussed. [ABSTRACT FROM AUTHOR]

Kittens in their first four weeks are in their most critical period because they do not yet have the ability to thermoregulate their bodies, and it is still difficult for them to adapt to environmental temperatures. Due to this condition, veterinary clinics and cat-lover communities need facilities that can maintain a kitten's body temperature within the normal range. One way to help in the care of these kittens is to use a special incubator for animals. Incubators are useful in situations where animals cannot control their body temperature conditions. The expected method to monitor the work system of the incubator is internet-based monitoring, as part of IoT (internet of things). Monitoring is very important for animal health workers and cat lovers in monitoring the temperature and humidity in the incubator using the internet, which allows monitoring to be carried out anytime and anywhere from a smartphone through the Blynk application. The purpose of this research is to create an IoT-based kitten incubator monitoring system through the blynk application so that the owner or nurse of the kitten can monitor in real time via a smartphone so that time efficiency can be improved, by using NodeMCU ESP8266 microcontroller with fuzzy logic method. The incubator can work automatically to regulate the temperature through lighting and air settings in it, with the applied temperature ranging from 26 degrees to 30 degrees Celsius. [ABSTRACT FROM AUTHOR]

Study Objectives: Ponto-geniculo-occipital (PGO) waves are a hallmark feature of paradoxical sleep (PS), emerging just before PS onset during the transition from slow wave sleep (SWS) to PS (TSWS-PS) and persisting throughout the PS phase in adult cats. These waves are considered a biomarker for the maturation of PS. However, their developmental trajectory in mammals remains poorly understood. This study investigated the emergence and maturation of PGO waves during PS in developing kittens., Methods: Electrodes were stereotaxically implanted into the lateral geniculate nucleus, accurately localized using magnetic resonance imaging (MRI). Recording included cortical electroencephalogram, nuchal muscle activity, PGO waves and rapid eye movements (REMs)., Results: Despite the presence of abundant PS and frequent muscle twitches accompanied by REMs in neonatal kittens, PGO spikes were first detected at postnatal day (PND) 16, with an average onset at PND 19. Initially, these waves appeared with lower density and amplitude during the TSWS-PS and PS phases. Over the following weeks, PGO wave density and amplitude increased, along with prolonged durations during both TSWS-PS and PS. Type I waves (singlet and bigeminy) predominated during TSWS-PS, while type II waves (clusters) were more frequent during PS. The proportion of type II waves and their co-occurrence with REMs increased with age. The adult-like pattern of PGO waves was fully established by PND 50., Conclusions: The emergence and maturation of PGO waves during PS are closely linked to the development of the sleep-wake cycle and may play a role in learning and memory processes., (© The Author(s) 2025. Published by Oxford University Press on behalf of Sleep Research Society. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site—for further information please contact journals.permissions@oup.com.)

Copyright of Kafkas Universitesi Veteriner Fakultesi Dergisi is the property of University of Kafkas, Faculty of Veterinary Medicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Birth weight is one of the earliest health parameters with short (neonatal period) and long term (adulthood) implications for an individual. The present work was conducted on the domestic cat, with the objective of determining factors affecting kitten birth weight. Data voluntarily shared by 139 French breeders allowed building a large dataset of purebred kittens (n = 3,547) from 15 breeds. A linear mixed model with queen and cattery as random terms was used to investigate variation factors of kitten birth weight such as breed, litter size, season of birth, age of the queen, presence of stillborn in the litter, sex of the kitten. The most important factor was breed which explained 25% of the variation in birth weight observed in the study population. The five other parameters were also significant but explained only a small additional part of the variance (less than 3% each). Analyses showed that kitten birth weight increased with the age of the queen, was higher in males than females and in litters without stillbirth than in litters with at least one stillborn. In addition, lower birth weights were recorded in summer and autumn compared to other seasons, and birth weight values decreased as the number of kittens in the litter increased. In order to group feline breeds according to their average birth weights and litter sizes, a K-means algorithm was used to identify three clusters among the 15 breeds represented (Group 1: small litter sizes/low birth weights; Group 2: large litter sizes/intermediate birth weights; Group 3: large litter sizes/high birth weights). This study, based on a large dataset established at the national scale, provides reference values of feline birth weights for breeders and veterinarians. The next step could be to explore the relationship between birth weight and neonatal mortality to help identify neonates requiring specific care. • The study describes birth weight values in purebred kittens. • Kitten birth weight is affected by breed, litter size, sex, season of birth, age of the queen and litter composition. • Three breed groups were formed on the basis of birth weight and litter size. [ABSTRACT FROM AUTHOR]

Abstract Purpose The present study compared the expression of early growth responsive gene-1 (Egr-1) in visual cortex between amblyopia kittens and normal kittens, and to explore the role of Egr-1 in the pathogenesis of amblyopia. Methods A total of 20 healthy kittens were randomly divided into deprivation group and control group with 10 kittens in each group. Raised in natural light, and covered the right eye of the deprived kittens with a black opaque covering cloth. Pattern visual evoked potentials (PVEP) were measured before and at the 1st, 3rd and 5th week after covering in all kittens. After the last PVEP test, all kittens were killed. The expression of Egr-1 in the visual cortex of the two groups was compared by immunohistochemistry and in situ hybridization. Results PVEP detection showed that at the age of 6 and 8 weeks, the P100 wave latency in the right eye of deprivation group was higher than that in the left eye of deprivation group (P

A two-month-old, male intact, mixed-breed cat weighing 0.6 kg was presented with respiratory distress and anorexia. From the transthoracic echocardiographic, reduced fractional shortening (FS) and increased endocardial echogenicity were recognised with severe congestive heart failure (CHF). The kitten was administered an antibiotic and pimobendane under oxygen supplementation in an ICU cage. However, the respiratory condition worsened and the cat died the next day, and the subsequent necropsy and histopathology examinations confirmed endocardial fibroelastosis (EFE). There is a lack of information regarding the antemortem cardiac function evaluated by tissue Doppler imaging (TDI) in EFE cases. We report on the echocardiographic findings including the TDI in the EFE cat with a concomitant necropsy and histopathology confirmation in this paper. The echocardiographic findings showed presence of a ventricular false tendon within the left ventricle, a decrease in the left ventricular contractility (FS 11.1%, and a marked CHF). In this case, the echocardiographic findings were consistent with the human counterpart. However, these findings were like those of dilated cardiomyopathy and, hence, non-specific to EFE. As a result, veterinarians should keep in mind that endocardial fibroelastosis might be a possible reason for respiratory distress resulting from CHF with a low fractional shortening in young cats. [ABSTRACT FROM AUTHOR]

Kittens are prone to intestinal health problems as their intestines are not completely developed. Seaweed is rich in plant polysaccharides and bioactive substances that are highly beneficial to gut health. However, the effects of seaweed on cat gut health have not been assessed. This study compared the effects of dietary supplementation with enzymolysis seaweed powder and Saccharomyces boulardii on the intestinal health of kittens. In total, 30 Ragdoll kittens (age: 6 months; weight: 1.50 ± 0.29 kg) were assigned to three treatment groups for a 4-week feeding trial. The dietary treatment given was as follows: (1) basal diet (CON); (2) CON + enzymolysis seaweed powder (20 g/kg of feed) mixed evenly with the diet (SE); and (3) CON + Saccharomyces boulardii (2 × 1010 CFU/kg of feed) mixed evenly with the diet (SB). Compared with the CON and SB groups, dietary supplementation with the enzymolysis seaweed powder improved the immune and antioxidant capacity and also reduced the intestinal permeability and inflammation levels of kittens. The relative abundance of Bacteroidetes, Lachnospiraceae, Prevotellaceae, and Faecalibacterium in the SE group was higher than those in the CON and SB groups (p ≤ 0.05), while the relative abundance of Desulfobacterota, Sutterellaceae, and Erysipelatoclostridium in the SB group was lower than that in the SE group (p ≤ 0.05). Moreover, enzymolysis seaweed powder did not alter the level of intestinal SCFAs in kittens. Conclusively, supplementing kitten diet with enzymolysis seaweed powder can promote intestinal health by enhancing the gut barrier function and optimizing the microbiota composition. Our findings provide new perspectives on the application of enzymolysis seaweed powder.

Case summary Two 18-day-old domestic shorthair kittens presented with dyspnoea, mild cyanosis, heart murmur, lung pattern, abnormal crawling and a marked dorsoventral flattening of the thorax. Deformity of the thoracic wall without pectus excavatum was diagnosed. Cardboard corsets tailored to each kitten were applied. This gave immediate respiratory relief, easing distress and pain. After 10 days, clinical and radiographic monitoring revealed normalisation of the shape of the chest wall with an improvement in general condition. At 6 months of age, clinical examination showed normal growth and development of both kittens, with a normal thoracic profile and shape also seen on radiographs. Relevance and novel information Data about flat-chested kitten syndrome and its aetiopathogenesis, treatment and outcome are scarce, but there is significant experience among breeders that is shared through online communities. High mortality rates are reported. The use of a cardboard splint appears to be novel in the published literature; in this case report, it proved to be a rapid and easy solution. For this reason, cardboard splints could be considered as a first approach for the clinical management of flat-chested kitten syndrome., Competing Interests: The authors declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article., (© The Author(s) 2024.)

Purpose: The present study compared the expression of early growth responsive gene-1 (Egr-1) in visual cortex between amblyopia kittens and normal kittens, and to explore the role of Egr-1 in the pathogenesis of amblyopia.Methods: A total of 20 healthy kittens were randomly divided into deprivation group and control group with 10 kittens in each group. Raised in natural light, and covered the right eye of the deprived kittens with a black opaque covering cloth. Pattern visual evoked potentials (PVEP) were measured before and at the 1st, 3rd and 5th week after covering in all kittens. After the last PVEP test, all kittens were killed. The expression of Egr-1 in the visual cortex of the two groups was compared by immunohistochemistry and in situ hybridization.Results: PVEP detection showed that at the age of 6 and 8 weeks, the P100 wave latency in the right eye of deprivation group was higher than that in the left eye of deprivation group (P < 0.05) and the right eye of control group (P < 0.05), while the amplitude decreased (P < 0.05). The number of positive cells (P < 0.05) and mean optical density (P < 0.05) of Egr-1 protein expression in visual cortex of 8-week-old deprivation group were lower than those of normal group, as well as the number (P < 0.05) and mean optical density of Egr-1 mRNA-positive cells (P < 0.05).Conclusions: Monocular form deprivation amblyopia can lead to the decrease of Egr-1 protein and mRNA expression in visual cortex, and then promote the occurrence and development of amblyopia. [ABSTRACT FROM AUTHOR]

A 4-month-old male indoor cat was referred for dyspnea, mental dullness and weakness, which appeared two days earlier. The cat had been adopted at 3 months of age. Physical exam showed cyanosis, dyspnea and mild hypothermia. The “spot test” was supportive of methemoglobinemia. Co-oximetry blood gas analysis revealed severe methemoglobinemia (81.40%), severe hyperchloremia and mild hyponatremia. CBC, biochemistry and urinalysis were within normal limits, blood smear showed the presence of Heinz bodies. Treatment was instituted with oxygen therapy, methylene blue 1% solution, ascorbic acid, intravenous fluid therapy. The clinical course was favorable with rapid improvement of cyanosis and methemoglobinemia (4.2%). Acquired methemoglobinemia was hypothesized. Two weeks after discharge the cat was asymptomatic but mild methemoglobinemia (15.60%) and hyperchloremia were evident. Exposure to oxidants contained in drinking water was suspected so the owners were instructed to use bottled water only. One month later the cat was asymptomatic, and methemoglobinemia and chloremia were within normal limits. Even if a congenital form due to cytochrome b5 reductase deficiency cannot be ruled out, drinking water intoxication is the most likely cause of methemoglobinemia in this cat. [ABSTRACT FROM AUTHOR]

The critical socialisation period for kittens is estimated to be 2–7 weeks of age. Kittens reared in shelters often have poorer outcomes due to high rates of infectious disease. Therefore, many shelters are reliant on foster care programs to improve kitten survival rates and behaviour. However, little is known about the impact of specific early socialisation factors on kitten behaviour and health. During a 23-month period, 598 kitten foster carers completed a kitten socialisation questionnaire. The relationships between kitten weight/age at intake, days in care, singleton status, and the presence or absence of the queen during care on kitten behaviour and disease incidence were explored. Associations with exposure to noise, adults, children, location where the kitten was kept in the home, time spent alone, and ringworm treatment were also investigated. Overall being kept in social areas of the house with less noise/activity and spending some time alone was associated with positive kitten behaviour. There was no difference in fear or anxiety responses for bottle fed kittens, singletons and those without the queen. Ringworm treatment was associated with more fearful behaviour in kittens. and relevance: Future foster carers should be encouraged to keep kittens in social areas of the house, whilst also ensuring kittens can spend some time alone. If the home is loud, carers should slowly introduce the kitten/s to the louder and busier areas of the house before being housed there all the time. Training of carers to use more positive reinforcement techniques for administering ringworm treatment may benefit kitten behaviour. • Kittens housed in quieter social areas was associated with positive behaviour. • For kittens, spending some time alone was associated with positive behaviour. • Bottle fed and single orphan kittens were no more likely to be fearful or aggressive. • Ringworm treatment was associated with more fearful behaviours in kittens. [ABSTRACT FROM AUTHOR]

Abstract Background Exploring the role of vasoactive intestinal peptide (VIP) in the lateral geniculate body (LGBd) in visual development and studying the therapeutic effect of VIP on amblyopic kittens. Methods Three-week-old domestic cats were divided into a control group (n = 10) and a monocular deprivation group (n = 20), with an eye mask covering the right eye of those in the deprived group. After pattern visual evoked potential (PVEP) recording confirmed the formation of monocular amblyopia, the left LGBd was isolated from 5 kittens in each group. The remaining control kittens continued to be raised, and the remaining deprivation group was divided into a VIP intervention group (n = 5), Sefsol (caprylic acid monoglyceride, VIP solution) intervention group (n = 5) and amblyopia non-intervention group (n = 5) after removal of the eye mask. Three weeks later, PVEPs, VIP immunohistochemistry and VIP mRNA expression in the left LGBd were compared across groups. Results At 6 weeks of age, there were significant differences in P100 wave latency and amplitude and VIP immunohistochemistry and in situ hybridization between the control group and the deprivation group (P 0.05), as well as the number (P

Copyright of Summa, Animali da Compagnia is the property of Point Veterinaire Italie s.r.l. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Summa, Animali da Compagnia is the property of Point Veterinaire Italie s.r.l. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Summa, Animali da Compagnia is the property of Point Veterinaire Italie s.r.l. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Summa, Animali da Compagnia is the property of Point Veterinaire Italie s.r.l. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The guidelines, authored by a Task Force of experts in feline clinical medicine, are an update and extension of the AAFP–AAHA Feline Life Stage Guidelines published in 2010. The guidelines are published simultaneously in the Journal of Feline Medicine and Surgery (volume 23, issue 3, pages 211–233, DOI: 10.1177/1098612X21993657) and the Journal of the American Animal Hospital Association (volume 57, issue 2, pages 51–72, DOI: 10.5326/JAAHA-MS-7189). A noteworthy change from the earlier guidelines is the division of the cat's lifespan into a five-stage grouping with four distinct age-related stages (kitten, young adult, mature adult, and senior) as well as an end-of-life stage, instead of the previous six. This simplified grouping is consistent with how pet owners generally perceive their cat's maturation and aging process, and provides a readily understood basis for an evolving, individualized, lifelong feline healthcare strategy. The guidelines include a comprehensive table on the components of a feline wellness visit that provides a framework for systematically implementing an individualized life stage approach to feline healthcare. Included are recommendations for managing the most critical health-related factors in relation to a cat's life stage. These recommendations are further explained in the following categories: behavior and environmental needs; elimination; life stage nutrition and weight management; oral health; parasite control; vaccination; zoonoses and human safety; and recommended diagnostics based on life stage. A discussion on overcoming barriers to veterinary visits by cat owners offers practical advice on one of the most challenging aspects of delivering regular feline healthcare. [ABSTRACT FROM AUTHOR]

Abstract Pentatrichomonas hominis and Tritrichomonas foetus (cat genotype) have been commonly identified as intestinal trichomonads in both dogs and cats. Although P. hominis is considered as non-pathogenic protozoa in many kinds of mammals, it has the potential for zoonotic transmission. T. foetus has been recognized as the emerging causative agent of diarrhea in cats without the risk of zoonotic transmission. As pet shops are the major source of young companion animals, the present study discusses the molecular prevalence of P. hominis and T. foetus from 544 pet shop puppies and 409 kittens. The results suggest that the prevalence of P. hominis (puppies: 7.0%; kittens: 0.5%) and T. foetus (puppies: 0%; kittens: 2.4%) in pet shop young animals are low. In addition, the infections of P. hominis and T. foetus are not always associated with the clinical signs (soft or diarrhea feces).

A 4- month- old domestic short hair (DSH) kitten with malformed, non weight bearing and painful left pelvic limb without any history of trauma was referred to small animal hospital. Hemimelia is a congenital complete or partial absence of one or more than one bone in limbs. All appendicular bones can be affected and many variations have been shown. Radial, tibial and ulnar hemimielia are usually common. In the present clinical report we found terminal hemimelia in the left hind limb along with agenesis of tibia, metatarsal bones and phalanges and agenesis of metatarsal bones and phalanges in the contralateral hindlimb. In treatment of tibial hemimelia, it may be possible to prevent permanent muscle contraction, bone deformation and varus but in this case, amputation of affected limb was recommended to the owner because of severe limb deformities, limb rotation and impossibility of arthrodesis. Castration/neutering to prevent congenital spread and radiography of other body parts to detect concurrent disorders is recommended in these cases.

Phimosis is usually result from congenital anomaly, trauma, and prepuce or penis tumor. The present case explained the occurrence of congenital phimosis in a 3 month male Scottish Fold Kitten. The kitten was presented with the history of oliguria and urinary incontinence. In physical examination, preputial swellings without urine discharge were observed. Other Clinical parameters, biochemical and complete blood count results were normal. The surgical treatment as the solve method for correction the phimosis, was done under general anesthesia and the little full thickness skin fold was removed from apex of prepuce. The skin and mucosa were opposed together with a simple interrupted suture pattern. The patient was followed for 1 mouth, after surgery, and there was no recurrence or post operation complication. Phimosis is reported in many animal species, but the most affected animal are dogs. There are few reports about feline phimosis, but no congenital cases were reported in Scottish Fold breed. [ABSTRACT FROM AUTHOR]

Copyright of Summa, Animali da Compagnia is the property of Point Veterinaire Italie s.r.l. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

The lungworm Aelurostrongylus abstrusus is one of the main causes of respiratory diseases in cats worldwide. This report describes the unusual case of a kitten infected with A. abstrusus and presented to a veterinary clinic in Brazil with lethargy, dysphagia, non-ambulatory tetraplegia, and pelvic limbs bilateral myoclonus. The clinical picture of the kitten worsened with generalized flaccid tetraplegia and death a few days after hospitalization. At necropsy, hemorrhagic necrosis and subarachnoid hemorrhages were detected in several areas of the central nervous system. Nematode stages were found at post-mortem histological examinations in lungs, cerebellum, subarachnoid space of the brain and spinal cord. Microscopic and molecular (PCRs-coupled-sequencing protocols) examination showed the presence of A. abstrusus in histological samples. This study describes the first neurological aelurostrongylosis due to ectopic localization of adult worms in the central nervous system of a cat, causing acute hemorrhagic multifocal meningoencephalomyelitis. Further studies are necessary to elucidate whether unusual localizations and the migration of A. abstrusus are more frequent than expected.

A 4-month-old male indoor cat was referred for dyspnea, mental dullness and weakness, which appeared two days earlier. The cat had been adopted at 3 months of age. Physical exam showed cyanosis, dyspnea and mild hypothermia. The “spot test” was supportive of methemoglobinemia. Co-oximetry blood gas analysis revealed severe methemoglobinemia (81.40%), severe hyperchloremia and mild hyponatremia. CBC, biochemistry and urinalysis were within normal limits, blood smear showed the presence of Heinz bodies. Treatment was instituted with oxygen therapy, methylene blue 1% solution, ascorbic acid, intravenous fluid therapy. The clinical course was favorable with rapid improvement of cyanosis and methemoglobinemia (4.2%). Acquired methemoglobinemia was hypothesized. Two weeks after discharge the cat was asymptomatic but mild methemoglobinemia (15.60%) and hyperchloremia were evident. Exposure to oxidants contained in drinking water was suspected so the owners were instructed to use bottled water only. One month later the cat was asymptomatic, and methemoglobinemia and chloremia were within normal limits. Even if a congenital form due to cytochrome b5 reductase deficiency cannot be ruled out, drinking water intoxication is the most likely cause of methemoglobinemia in this cat.

Objective: The expression of early growth responsive gene-1 (Egr-1) in the lateral geniculate body in the normal kittens and those affected with amblyopia caused by monocular visual deprivation was compared to explore the potential significance of Egr-1 in the pathogenesis of amblyopia., Methods: A total of 30 healthy kittens were equally and randomly divided into the control ( n  = 15) and the deprivation group ( n  = 15). The kittens were raised in natural light and the right eyes of the deprived kittens were covered with a black opaque covering. Pattern visual evoked potential (PVEP) was measured before and 1, 3, and 5 weeks after covering. Five kittens from each group were randomly selected and euthanized with 2% sodium pentobarbital (100 mg/kg) during the 1st, 3rd and 5th week after covering. The expression of Egr-1 in the lateral geniculate body in the two groups was compared by performing immunohistochemistry and in situ hybridization., Results: After three weeks of covering, PVEP detection indicated that the P100 wave latency in the deprivation group was significantly higher than that in the control group (P < 0.05), whereas the amplitude decreased markedly (P < 0.05). The number of the positive cells (P < 0.05) and mean optical density (P < 0.05) of Egr-1 protein expression in the lateral geniculate body of the deprivation group were found to be substantially lower in comparison to the normal group, as well as the number (P < 0.05) and mean optical density of Egr-1 mRNA-positive cells (P < 0.05). However, with increase of age, positive expression of Egr-1 in the control group showed an upward trend (P < 0.05), but this trend was not noted in the deprivation group (P > 0.05)., Conclusions: Monocular form deprivation can lead to substantially decreased expressions of Egr-1 protein and mRNA in the lateral geniculate body, which in turn can affect the normal expression of neuronal functions in the lateral geniculate body, thereby promoting the occurrence and development of amblyopia., Competing Interests: Declaration of conflicting interestsThe author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article.