355 results on '"Kiyotake Suenaga"'
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2. Allelopathic Activity of Leaf Wastes of Liriodendron tulipifera for Sustainable Management
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Hisashi Kato-Noguchi, Kota Hayashi, Arihiro Iwasaki, and Kiyotake Suenaga
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allelochemical ,industrial waste ,lipiferolide ,ornamental tree ,soil mixture ,sustainability ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Logging operations of Liriodendron tulipifera L., as timber trees, and fallen leaves in autumn from ornamental trees produce a large amount of leaf waste. In this study, the allelopathy of L. tulipifera fresh and fallen leaves was investigated for the development of potential applications of leaf waste. The extracts of fresh and fallen leaves of L. tulipifera showed growth inhibitory activity against weed species, Vulpia myuros (L.) C.C.Gmel., Echinochloa crus-galli (L.) P.Beauv., and Lolium multiflorum Lam., under laboratory conditions. The powder of L. tulipifera fresh and fallen leaves also inhibited the germination of E. crus-galli under greenhouse conditions. A potent allelochemical was isolated from fresh and fallen leaf extracts through a bioassay-guided separation process, and was identified as lipiferolide. Lipiferolide inhibited the growth of L. multiflorum and Lepidum sativum in a concentration-dependent manner. This investigation suggests that the leaf waste of L. tulipifera from logging operations and fallen leaves is potentially useful for the purpose of weed control, such as through the use of soil additive materials from leaves or the creation of foliar spray from leaf extracts. The development of weed control materials using L. tulipifera leaf waste may be a means with which to minimize waste, reducing environmental impacts and economic concerns.
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- 2024
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3. Allelopathy and Allelochemicals in Chamaecyparis obtusa Leaves for the Development of Sustainable Agriculture
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Hisashi Kato-Noguchi, Kumpei Mori, Arihiro Iwasaki, and Kiyotake Suenaga
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allelochemical ,(+)-dihydrosesamin ,eco-friendly agriculture ,hinokiic acid ,soil additive ,timber processing waste ,Agriculture - Abstract
Chamaecyparis obtusa (Siebold et Zucc.) Endl. is cultivated in the temperate region of East Asia for its high-quality and profitable timber. The timber-processing industry produces a large amount of waste, such as tree bark, branches, and leaves, and finding ways to minimize such waste is economically and environmentally efficient. In the present study, the allelopathic activity and allelochemicals of the C. obtusa leaves were investigated to develop potential application methods. C. obtusa leaves were phytotoxic and associated leaf extracts significantly suppressed the growth of the weeds; Echinochloa crus-galli, Phleum pratense and Lolium multiflorum under laboratory conditions. The leaf powder applied onto the soil surface also suppressed the germination of E. crus-galli and P. pratense under greenhouse conditions. Hinokiic acid and (+)-dihydrosesamin were isolated from the leaves and structurally identified. Both compounds suppressed the growth of cress and P. pratense in a concentration dependent manner. When the leaves of C. obtua were applied as a soil additive, hinokiic acid and (+)-dihydrosesamin in the leaves potentially cause the growth inhibitory activity by leaching into the soil. These experiments showed that the leaves and the leaf extracts of C. obtusa are phytotoxic. These experiments also demonstrated that the leaves and the leaf extracts of C. obtusa have growth inhibitory potential against several plant species, and the leaves produce allelochemicals. Therefore, the leaves and leaf extracts of C. obtusa may be useful for weed suppression purposes. The leaf biomass of C. obtusa is frequently large and the timber processing industry produces significant leaf waste. The development of weed control products using waste leaves may be a solution to minimize the timber processing waste to reduce environmental impact and provide economic value. However, leaf material should be evaluated for weed suppression and herbicidal activity as a soil additive and also possible as a foliar spray under field conditions.
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- 2024
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4. Allelopathic Potential of Marsdenia tenacissima (Roxb.) Moon against Four Test Plants and the Biological Activity of Its Allelopathic Novel Compound, 8-Dehydroxy-11β-O-Acetyl-12β-O-Tigloyl-17β-Marsdenin
- Author
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Seinn Moh Moh, Naoaki Kurisawa, Kiyotake Suenaga, and Hisashi Kato-Noguchi
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Marsdenia tenacissima ,allelopathic substances ,growth inhibition ,novel compound (steroidal glycoside 3 ,8-dehydroxy-11β-O-acetyl-12β-O-tigloyl-17β-marsdenin) ,Botany ,QK1-989 - Abstract
Plant parts and extracts that are rich in bioactive substances with allelopathic potential can be explored as a possible alternative to herbicides for natural weed control in sustainable agriculture. In the present study, we investigated the allelopathic potential of Marsdenia tenacissima leaves and its active substances. Aqueous methanol extracts of M. tenacissima showed significant inhibitory activities against the growth of lettuce (Lactuca sativa L.), alfalfa (Medicago sativa L.), timothy (Phleum pratense L.), and barnyard grass (Echinochloa crusgalli (L.) Beauv.). The extracts were purified through various chromatography steps, and one active substance was isolated and determined by spectral data to be a novel compound, assigned as steroidal glycoside 3 (8-dehydroxy-11β-O-acetyl-12β-O-tigloyl-17β-marsdenin). Steroidal glycoside 3 significantly inhibited the seedling growth of cress at a concentration of 0.03 mM. The concentrations needed for 50% growth inhibition of the cress shoots and roots were 0.25 and 0.03 mM, respectively. These results suggest that steroidal glycoside 3 may be responsible for the allelopathy of M. tenacissima leaves.
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- 2023
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5. Isolation and Identification of Plant-Growth Inhibitory Constituents from Polygonum chinense Linn and Evaluation of Their Bioherbicidal Potential
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Thang Lam Lun, Arihiro Iwasaki, Kiyotake Suenaga, and Hisashi Kato-Noguchi
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Polygonum chinense ,allelopathic substances ,(−)-3-hydroxy-β-ionone ,(−)-3-hydroxy-7,8-β-ionone ,Botany ,QK1-989 - Abstract
Polygonum chinense Linn. is a medicinal and invasive plant that belongs to the family Polygonaceae. The pharmacological activities and phytochemical constituents of Polygonum chinense are well reported, but the allelopathic effects and potent allelopathic substances of P. chinense remain to be investigated. Hence, this experiment was conducted to separate and characterize potentially allelopathic substances from an extract of the Polygonum chinense plant. The Polygonum chinense plant extracts highly suppressed the growth of cress (Lepidium sativum L.), lettuce (Lactuca sativa L.), barnyard grass (Echinochloa crusgalli (L.) P. Beauv.), and timothy grass (Phleum pratense L.) seedlings in a species- and concentration-dependent way. Two active substances were separated using a series of purification procedures and determined through spectral analysis as (−)-3-hydroxy-β-ionone and (−)-3-hydroxy-7,8-dihydro-β-ionone. These two compounds significantly suppressed the seedling growth of Lepidium sativum (cress) at concentrations of 0.01 and 1 mM, respectively. The extract concentrations necessary for 50% growth inhibition (I50 values) of the cress hypocotyls and roots were 0.05 and 0.07 mM for (−)-3-hydroxy-β-ionone, respectively, and 0.42 and 1.29 mM for (−)-3-hydroxy-7,8-β-ionone, respectively. These findings suggest that these two compounds are in charge of the inhibitory effects of the Polygonum chinense extract and may serve as weed control agents.
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- 2023
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6. Allelopathic Substances of Osmanthus spp. for Developing Sustainable Agriculture
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Hisashi Kato-Noguchi, Yuri Hamada, Misuzu Kojima, Sanae Kumagai, Arihiro Iwasaki, and Kiyotake Suenaga
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allelochemical ,10-acetoxyligustroside ,decomposition ,fallen leaf ,growth inhibition ,Osmanthus ,Botany ,QK1-989 - Abstract
Osmanthus fragrans Lour. has been cultivated for more than 2500 years because of the fragrance and color of the flowers. The flowers and roots have been used in tea, liquors, foods, and traditional Chinese medicine. The species contains more than 180 compounds including terpenoids, phenylpropanoids, polyphenols, flavonoids, and sterols. However, there has been limited information available on the allelopathic properties and allelopathic substances of O. fragrans. We investigated the allelopathy and allelopathic substances of O. fragrans and Osmanthus heterophyllus (G.Don) P.S. Green, as well as Osmanthus × fortunei Carrière, which is the hybrid species between O. fragrans and O. heterophyllus. The leaf extracts of O. fragrans, O. heterophyllus, and O. × fortunei suppressed the growth of cress (Lepidium sativum L.), alfalfa (Medicago sativa L.), Lolium multiflorum Lam., and Vulpia myuros (L.) C.C.Gmel with the extract concentration dependently. The extract of the hybrid species O. × fortune was the most active among the extracts. The main allelopathic substances of O. × fortunei and O. fragrans were isolated and identified as (+)-pinoresinol and 10-acetoxyligustroside, respectively. (+)-Pinoresinol was also found in the fallen leaves of O. × fortunei. Both compounds showed an allelopathic activity on the growth of cress and L. multiflorum. On the other hand, several allelopathic substances including (+)-pinoresinol may be involved in the allelopathy of O. heterophyllus. O. fragrans, O. heterophyllus, and O. × fortunei are evergreen trees. but their senescent leaves fall and cover the soil under the trees. It is possible that those allelopathic substances are liberated through the decomposition process of the leaves into their rhizosphere soil, and that they accumulate in the soil and provide a competitive advantage to the species through the inhibition of the growth of the neighboring competing plants. Therefore, the leaves of these Osmanthus species are allelopathic and potentially useful for weed management options in some agriculture settings to reduce commercial herbicide dependency for the developing sustainable agriculture systems.
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- 2023
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7. Assessment of the Phytotoxic Potential of Dregea volubilis (L.f.) Benth. ex Hook.f. and Identification of its Phytotoxic Substances for Weed Control
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Ei Han Kyaw, Arihiro Iwasaki, Kiyotake Suenaga, and Hisashi Kato-Noguchi
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leaf extracts ,growth inhibition ,3-hydroxy-α-ionone ,5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one ,Agriculture (General) ,S1-972 - Abstract
The phytotoxic potential of plants and the effect of their active components on another plant species is being explored as a potential alternative to synthetic herbicides for weed control. In the current study, we investigated the phytotoxic potential of the leaves of Dregea volubilis (L.f.) Benth. ex Hook.f. against four test plants [timothy (Phleum pratense L.), barnyard grass (Echinochloa crus-galli (L.) P. Beauv), lettuce (Lactuca sativa L.), and alfalfa (Medicago sativa L.)] and observed significant growth inhibition on those plants at concentrations >3 mg D.W. equivalent extract mL−1. A bioassay-governed purification of the D. volubilis extracts using different chromatography phases produced two growth inhibitory compounds, 3-hydroxy-α-ionone (compound 1) and 5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one (compound 2). The compounds retarded the growth of barnyard grass and cress (Lepidium sativum L.) with I50 (concentration required for 50% growth suppression) values ranging from 0.098 to 0.450 mM for 3-hydroxy-α-ionone and 0.029 to 0.420 mM for 5-hydroxy-3,4-dimethyl-5-pentylfuran-2(5H)-one. Thus, the extracts and identified compounds may have the possibility to be utilized as bioagents for weed control.
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- 2022
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8. Evaluation of phytotoxic potential and identification of phytotoxic substances in Cassia alata Linn. leaves
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Krishna Rany Das, Arihiro Iwasaki, Kiyotake Suenaga, and Hisashi Kato-Noguchi
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cassia alata ,phytotoxic substances ,rutin ,syringone ,eco-friendly weed management ,Plant culture ,SB1-1110 - Abstract
Cassia alata (Caesalpiniaceae) is a large perennial shrub native to tropical America. It is an important medicinal and ornamental plant and has been reported to possess several pharmacological properties. However, phytotoxic substances from C. alata have not yet been documented in the literature. Therefore, this study seeks to evaluate the phytotoxic potential of C. alata leaves and to identify inhibitory substances for the purpose of eco-friendly weed management. The effect of aqueous methanol extracts of C. alata on the seedling growth of alfalfa, cress, lettuce, rapeseed, barnyard grass, foxtail fescue, Italian ryegrass, and timothy was examined. The level of inhibition of the extracts corresponded to concentration. Several chromatographic steps were performed to separate the extracts, and through spectroscopic analysis, two active substances were isolated and characterised as rutin and syringone. These two active substances significantly inhibited the seedling growth of cress and foxtail fescue. The range of I50 values (required concentration for 50% growth inhibition) of rutin and syringone for the seedling growth of cress and foxtail fescue were 129.5–417.8 and 160.1–466.5 µM, respectively. These results indicate that the two identified active phytotoxic substances from C. alata may be responsible for its growth inhibitory properties.
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- 2019
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9. Phytotoxic Effects of Senna garrettiana and Identification of Phytotoxic Substances for the Development of Bioherbicides
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Ramida Krumsri, Arihiro Iwasaki, Kiyotake Suenaga, and Hisashi Kato-Noguchi
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biological activity ,bioherbicide ,growth inhibition ,phytotoxic compounds ,Agriculture (General) ,S1-972 - Abstract
Phytotoxic substances in plants that may serve as alternative natural herbicides for controlling weeds are required for sustainable agriculture. We explored the phytotoxic activities of aqueous methanol extracts of Senna garrettiana (Craib) Irwin & Barneby leaves and the active substances they contain. The results revealed that the S. garrettiana leaf extracts had significant phytotoxic effects on three dicotyledons (Lepidium sativum L., Lactuca sativa L., and Medicago sativa L.) and two monocotyledons (Phleum pratense L. and Lolium multiflorum Lam.). An bioassay-guided isolation process yielded three active substances; caffeic acid, methyl caffeate, and (S)-6-hydroxymellein inhibited the seed germination, seedling growth, and biomass accumulation of L. sativum in a concentration-dependent manner. Based on the concentration required for 50% growth inhibition (IC50), (S)-6-hydroxymellein had the highest inhibitory effects on L. sativum in all test parameters, followed by methyl caffeate and caffeic acid. The L. sativum roots were the most susceptible to (S)-6-hydroxymellein (IC50 = 383 µM) and caffeic acid (IC50 = 2627 µM), whereas methyl caffeate (IC50 = 1361 µM) had the greatest effect on the L. sativum shoots. Thus, three isolated compounds may explain the phytotoxic effects of the S. garrettiana extracts. Consequently, caffeic acid, methyl caffeate, and (S)-6-hydroxymellein could be potential candidates for the future production of bioherbicides.
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- 2022
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10. Two Allelopathic Substances from Plumbago rosea Stem Extracts and Their Allelopathic Effects
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Thang Lam Lun, Arihiro Iwasaki, Kiyotake Suenaga, and Hisashi Kato-Noguchi
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Plumbago rosea ,allelopathic substances ,7,4′,5′-tri-O-methyl dihydroquercetin ,7,4′,5′-tri-O-methylampelopsin ,Agriculture - Abstract
The plant Plumbago rosea Linn., belonging to the Plumbaginaceae family, is an important medicinal herb distributed in part of Southeast Asia, and there are many reports of its pharmacological properties. However, the allelopathic activities of P. rosea have not been examined. Thus, the present study was conducted to assess the allelopathic activity of P. rosea and to identify its allelopathic substances. The aqueous methanol stem extract of P. rosea significantly suppressed the seedling growth of barnyard grass (Echinochloa crus-galli L. P. Beauv.), Italian ryegrass (Lolium multiflorum Lam.), timothy (Phleum pretense L.), cress (Lepidium sativum L.), lettuce (Lactuca sativa L.), and alfalfa (Medicago sativa L.). The extract of P. rosea was then purified through chromatographic steps, and two active substances were isolated and determined as 7,4′,5′-tri-O-methyl dihydroquercetin and 7,4′,5′-tri-O-methylampelopsin. The two compounds significantly inhibited the seedling growth of cress, with 7,4′,5′-tri-O-methylampelopsin showing a greater inhibitory effect than 7,4′,5′-tri-O-methyl dihydroquercetin. This result may be due to the 3′-OH group in 7,4′,5′-tri-O-methylampelopsin. The effective concentrations of both compounds required for 50% growth inhibition (EC50 values) of cress seedlings were 0.24 mM and 0.59 mM for root and shoot, and 0.07 mM and 0.21 mM, respectively. These findings suggest that the two compounds may contribute to the allelopathic effect of P. rosea and could be used as a natural source of allelopathic substances.
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- 2022
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11. Allelopathic Activity of a Novel Compound, 5,6-Dihydrogen-11α-O-acetyl-12β-O-tigloyl-17β-marsdenin, and a Known Steroidal Glycoside from the Leaves of Marsdenia tenacissima (Roxb.) Moon
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Seinn Moh Moh, Arihiro Iwasaki, Kiyotake Suenaga, and Hisashi Kato-Noguchi
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Marsdenia tenacissima ,allelopathic substance ,medicinal plant ,steroidal glycoside ,Agriculture - Abstract
Medicinal plants are rich sources of bioactive substances that can be used to develop environmentally friendly weed control alternatives. Marsdenia tenacissima (Roxb.) Moon is a traditional medicinal plant well known for its pharmacological activities and several bioactive compounds. However, its allelopathy and related substances have not been reported. Hence, the present study was conducted to explore the allelopathic potential and substances from M. tenacissima leaves. Aqueous methanol extracts of M. tenacissima showed significant inhibitory activities against the growth of cress (Lepidium sativum L.) and Italian ryegrass (Lolium multiflorum Lam.). The extracts were purified through various chromatography steps, and two allelopathic substances were isolated and determined by spectral data to be steroidal glycoside 1 (5,6-dihydrogen-11α-O-acetyl-12β-O-tigloyl-17β-marsdenin), a novel compound, and steroidal glycoside 2 (5,6-dihydrogen-11α,12β-di-O-tigloyl-17β-marsdenin). Both compounds significantly inhibited the growth of cress seedlings. Steroidal glycoside 1 showed 1.6- and 4-times greater growth inhibitory potential against the cress shoots and roots than steroidal glycoside 2. The concentrations needed for 50% growth inhibition of the cress seedling shoots and roots were 0.46 and 0.03 mM for steroidal glycoside 1, respectively, and 0.74 and 0.12 mM for steroidal glycoside 2, respectively. Therefore, these results suggest that steroidal glycosides 1 and 2 may be responsible for the allelopathy of the M. tenacissima leaves.
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- 2022
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12. Assessment of Allelopathic Potential of Senna garrettiana Leaves and Identification of Potent Phytotoxic Substances
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Ramida Krumsri, Arihiro Iwasaki, Kiyotake Suenaga, and Hisashi Kato-Noguchi
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phytotoxic activity ,phenolic compounds ,bioherbicide ,sustainable agriculture ,Agriculture - Abstract
Senna garrettiana (Craib) Irwin & Barneby (Fabaceae) is a medicinal plant known to be rich in biologically active compounds that could be exploited to produce bioherbicides. The present study was conducted to explore the allelopathic potential and phytotoxic substances of S. garrettiana. Extracts of S. garrettiana leaves were found to significantly inhibit the growth of Lepidium sativum L. and Echinochloa crus-galli (L.) P. Beauv. (p ≤ 0.05). The phytotoxic substances were isolated and identified as vanillic acid and ferulic acid by bioassay-directed fractionation and spectral data analysis. The two compounds were shown to significantly inhibit the seed germination, seedling growth, and dry biomass of L. sativum. Based on the concentration required for 50% growth inhibition (defined as IC50), the roots of L. sativum were the most sensitive to the compounds, and the inhibitory effect of ferulic acid (IC50 = 0.62 mM) was >1.3 times more potent than that of vanillic acid (IC50 = 0.82 mM). In addition, a mixture of the two compounds (0.3 mM) resulted in synergistic inhibitory activity against the L. sativum roots compared with the individual compounds. These results suggest that the extracts of S. garrettiana leaves and their phytotoxic compounds have potential as candidate natural herbicides.
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- 2022
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13. Allelopathy of the Medicinal Plant Dregea volubilis (L.f.) Benth. ex Hook.f. and Its Phytotoxic Substances with Allelopathic Activity
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Ei Han Kyaw, Arihiro Iwasaki, Kiyotake Suenaga, and Hisashi Kato-Noguchi
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Dregea volubilis ,allelopathic effect ,phytotoxic substances ,dehydrovomifoliol ,loliolide ,Agriculture - Abstract
Allelopathic plants and their related phytotoxic chemicals have been explored as alternatives for environmentally friendly weed management. Dregea volubilis (L.f.) Benth. ex Hook.f., a woody vine, is well-known for its uses in various therapeutic treatments, and several bioactive compounds have been isolated from this species. However, to date, no phytotoxic substances from D. volubilis have been reported. Hence, in this research, we aimed to investigate the allelopathic effect of D. volubilis and characterize its phytotoxic substances. Extracts of D. volubilis exhibited significant inhibitory activity against the seedling growth of Italian ryegrass (Lolium multiflorum Lam.) and cress (Lepidium sativum L.). The extracts were purified through several chromatography steps, yielding two phytotoxic substances, and using spectroscopy, their structures were determined as dehydrovomifoliol and loliolide. The compounds inhibited the Italian ryegrass and cress seedlings with the I50 (concentrations needed for 50% inhibition) values ranging from 0.022 to 0.102 mM, for loliolide and 3.24–4.60 mM, for dehydrovomifoliol. These results suggest that both phytotoxic compounds might be responsible for the allelopathy of D. volubilis, and they may be released into the soil through the decomposition of the D. volubilis leaf and probably act as allelopathic active substances. Therefore, the extracts of D. volubilis and its related phytotoxic compounds may be applied as biological agents for controlling weeds.
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- 2022
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14. Two allelopathic substances from Paspalum commersonii Lam.
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Farhana Zaman, Arihiro Iwasaki, Kiyotake Suenaga, and Hisashi Kato-Noguchi
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paspalum ,allelopathy ,growth inhibitor ,dehydrovomifoliol ,loliolide ,Plant culture ,SB1-1110 - Abstract
Paspalum commersonii (Poaceae) is a herbaceous perennial weed distributed in the tropics and subtropics regions and grows mainly in the moist, or even flooded soil. It often appears in the rice field as a competitive weed and difficult to manage. Its strong competitive nature indicates possible allelopathic potential of P. commersonii. However, no studies have been found yet on the allelopathic activity of P. commersonii. Thus, we investigated the allelopathic potential of this weed and determined its allelopathic substances. Aqueous methanol extracts of P. commersonii showed concentration-dependent inhibitory activity on the seedling growth of cress, alfalfa, rapeseed, lettuce, barnyard grass, foxtail fescue, Italian ryegrass, and timothy. Two substances were isolated through bioassay-guided fractionation and their structures were determined through spectral data as dehydrovomifoliol and loliolide. Dehydrovomifoliol and loliolide started inhibiting the shoot and root growth of cress at concentrations greater than 3 and 0.03 mM, respectively. The concentrations required for 50% inhibition (I50) of cress shoot and root growth were 3.34 and >3.50 mM for dehydrovomifoliol and 0.04 and 0.05 mM for loliolide, respectively. These results indicate that both substances may affect the inhibitory activity of P. commersonii.
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- 2018
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15. Allelopathy and Allelopathic Substances of Fossil Tree Species Metasequoia glyptostroboides
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Yuki Matuda, Arihiro Iwasaki, Kiyotake Suenaga, and Hisashi Kato-Noguchi
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allelopathy ,fallen leaf ,fossil tree ,decomposition ,mycorrhizal colonization ,growth inhibition ,Agriculture - Abstract
Metasequoia glyptostroboides Hu et W.C. Cheng is one of the oldest living conifer species, and it has remained unchanged for millions of years compared to its fossils from the Cretaceous period. The species are cultivated in the parks, gardens, and roadsides in many countries. We investigated the allelopathy and allelopathic substances in fallen leaves of M. glyptostroboides. An aqueous methanol extract of the fallen leaves inhibited the growth of cress (Lepidium sativum L.), lettuce (Lactuca sativa L.), alfalfa (Medicago sativa L.), Lolium multiflorum Lam., Phleum pretense L., and Vulpia myuros (L.) C.C.Gmel. The extract was then purified by several chromatographic steps, and two allelopathic substances were isolated and determined by spectral data to be (+)-rhododendrol and 9-epi-blumenol C. The compound inhibited the growth of cress and L. multiflorum. M. glyptostroboides is a deciduous perennial tree, and accumulation of its fallen leaves occur on the soil under the trees. Therefore, those allelopathic substances in the fallen leaves may be liberated into the rhizosphere soil during the decomposition process of fallen leaves and provide a competitive advantage for M. glyptostrob through the growth inhibition of competing plant species nearby. Therefore, M. glyptostroboides is allelopathic, and (+)-rhododendrol and 9-epi-blumenol C may be contribute to the allelopathy.
- Published
- 2021
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16. Metabolomic Characterization of a cf. Neolyngbya Cyanobacterium from the South China Sea Reveals Wenchangamide A, a Lipopeptide with In Vitro Apoptotic Potential in Colon Cancer Cells
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Lijian Ding, Rinat Bar-Shalom, Dikla Aharonovich, Naoaki Kurisawa, Gaurav Patial, Shuang Li, Shan He, Xiaojun Yan, Arihiro Iwasaki, Kiyotake Suenaga, Chengcong Zhu, Haixi Luo, Fuli Tian, Fuad Fares, C. Benjamin Naman, and Tal Luzzatto-Knaan
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metabolomics ,secondary metabolites ,natural products ,cyanobacteria ,Neolyngbya ,anticancer ,Biology (General) ,QH301-705.5 - Abstract
Metabolomics can be used to study complex mixtures of natural products, or secondary metabolites, for many different purposes. One productive application of metabolomics that has emerged in recent years is the guiding direction for isolating molecules with structural novelty through analysis of untargeted LC-MS/MS data. The metabolomics-driven investigation and bioassay-guided fractionation of a biomass assemblage from the South China Sea dominated by a marine filamentous cyanobacteria, cf. Neolyngbya sp., has led to the discovery of a natural product in this study, wenchangamide A (1). Wenchangamide A was found to concentration-dependently cause fast-onset apoptosis in HCT116 human colon cancer cells in vitro (24 h IC50 = 38 μM). Untargeted metabolomics, by way of MS/MS molecular networking, was used further to generate a structural proposal for a new natural product analogue of 1, here coined wenchangamide B, which was present in the organic extract and bioactive sub-fractions of the biomass examined. The wenchangamides are of interest for anticancer drug discovery, and the characterization of these molecules will facilitate the future discovery of related natural products and development of synthetic analogues.
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- 2021
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17. Allelopathic potential and an allelopathic substance in mango leaves
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Masahiko Suzuki, Md Sirajul Islam Khan, Arihiro Iwasaki, Kiyotake Suenaga, and Hisashi Kato-Noguchi
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allelopathy ,growth inhibition ,mango ,methyl gallate ,allelopathic substance ,Plant culture ,SB1-1110 - Abstract
The present study was aimed to determine the allelopathic potential of mango (Mangifera indica L.) leaves and to identify allelopathic substances in the leaves. The aqueous methanol extracts of mango leaves inhibited seedling growth of garden cress, radish, rapeseed, foxtail fescue and crabgrass, and the inhibitory effects increased with the increasing extract concentration, suggesting that mango leaves may contain allelopathic substances. The extract was then purified by several chromatographic runs through a bioassay-directed fractionation, and an growth inhibitory substance was isolated and identified by spectral data as methyl-3,4,5-trihydroxybenzoate (methyl gallate). Methyl gallate at the concentration of 10 mM inhibited 98.6% and 99.8% of root and shoot growth of garden cress relative to the control, respectively, and 94.4% and 49.3% of those of foxtail fescue, respectively. The concentrations of methyl gallate required for 50% growth inhibition (I50) on garden cress roots and shoots were 3.9 and 3.3 mM, and those on foxtail fescue roots and shoots were 1.5 and 9.5 mM, respectively. It is the first report of an allelopathic substance in mango leaves and allelopathic activity of methyl gallate. Therefore, the mango leaves may have potential as a soil additive material for the weed management options.
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- 2017
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18. Identification and Application of Bioactive Compounds from Garcinia xanthochymus Hook. for Weed Management
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Md. Mahfuzur Rob, Kawsar Hossen, Mst. Rokeya Khatun, Keitaro Iwasaki, Arihiro Iwasaki, Kiyotake Suenaga, and Hisashi Kato-Noguchi
- Subjects
Garcinia xanthochymus ,growth inhibitory compounds ,allelopathy ,vanillic acid ,methyl phloretate ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
The allelopathic potential of plant species and their related compounds has been increasingly reported to be biological tools for weed control. The allelopathic potential of Garcinia xanthochymus was assessed against several test plant species: lettuce, rapeseed, Italian ryegrass, and timothy. The extracts of G. xanthochymus leaves significantly inhibited all the test plants in a concentration- and species-specific manner. Therefore, to identify the specific compounds involved in the allelopathic activity of the G. xanthochymus extracts, assay-guided purification was carried out and two allelopathic compounds were isolated and identified as methyl phloretate {3-(4-hydroxyphenyl) propionic acid methyl ester} and vanillic acid (4-hydroxy-3-methoxybenzoic acid). Both of the substances significantly arrested the cress and timothy seedlings growth. I50 values (concentrations required for 50% inhibition) for shoots and roots growth of the cress and timothy were 113.6–104.6 and 53.3–40.5 μM, respectively, for methyl phloretate, and 331.6–314.7 and 118.8–107.4 μM, respectively, for vanillic acid, which implied that methyl phloretate was close to 3- and 2-fold more effective than vanillic acid against cress and timothy, respectively. This report is the first on the presence of methyl phloretate in a plant and its phytotoxic property. These observations suggest that methyl phloretate and vanillic acid might participate in the phytotoxicity of G. xanthochymus extract.
- Published
- 2021
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19. Phytotoxic Activity and Growth Inhibitory Substances from Albizia richardiana (Voigt.) King & Prain
- Author
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Kawsar Hossen, Arihiro Iwasaki, Kiyotake Suenaga, and Hisashi Kato-Noguchi
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Albizia richardiana ,sustainable agriculture ,allelopathic substances ,dehydrovomifoliol ,loliolide ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Albizia richardiana, a fast-growing, large deciduous tree belonging to the Fabaceae family, grows well in hot and humid areas but mainly grows in the tropics of the Old World. The medicinal and other uses of Albizia richardiana are well documented, but the phytotoxic effects of this tree have not yet been investigated. We conducted this study to investigate the phytotoxic activity of Albizia richardiana leaves and to identify growth inhibitory substances for controlling weeds in a sustainable way. Aqueous methanol extracts of Albizia richardiana leaves greatly suppressed the growth of cress and barnyard grass seedlings in a concentration- and species-dependent manner. Two phytotoxic substances were separated using several purification steps and characterized through spectral analysis as dehydrovomifoliol and loliolide. Dehydrovomifoliol and loliolide significantly arrested the seedling growth of cress in the concentrations of 0.1 and 0.01 mM, respectively. The extract concentrations needed for 50% growth inhibition (I50 values) of cress seedlings were 3.16–3.01 mM for dehydrovomifoliol and 0.03–0.02 mM for loliolide. The results suggest that these two allelopathic substances might play a vital role in the phytotoxicity of Albizia richardiana leaves.
- Published
- 2021
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20. Allelopathic Potential and Active Substances from Wedelia Chinensis (Osbeck)
- Author
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Kawsar Hossen, Krishna Rany Das, Shun Okada, Arihiro Iwasaki, Kiyotake Suenaga, and Hisashi Kato-Noguchi
- Subjects
Wedelia chinensis ,organic farming ,phytotoxic substances ,vanillic acid ,gallic acid ,Chemical technology ,TP1-1185 - Abstract
Wedelia chinensis (Asteraceae) is a wetland herb native to India, China, and Japan. It is a valuable medicinal plant recorded to have pharmaceutical properties. However, the phytotoxic potential of Wedelia chinensis has not yet been examined. Thus, we carried out this study to establish the allelopathic effects of Wedelia chinensis and to identify its phytotoxic substances. Extracts of Wedelia chinensis exhibited high inhibitory activity against the root and shoot growth of cress, alfalfa, rapeseed, lettuce, foxtail fescue, Italian ryegrass, timothy, and barnyard grass. The inhibition was varied with species and was dependent on concentrations. The extracts were separated through several purification steps, and the two effective substances were isolated and characterized as vanillic acid and gallic acid using spectral analysis. Vanillic acid and gallic acid significantly arrested the growth of cress and Italian ryegrass seedlings. The concentrations of vanillic acid and gallic acid needed for 50% inhibition (I50 values) of the seedling growth of the cress and Italian ryegrass were 0.04–15.4 and 0.45–6.6 mM, respectively. The findings suggest that vanillic acid and gallic acid may be required for the growth inhibitory activities of Wedelia chinensis.
- Published
- 2020
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21. Phytotoxic Activity and Identification of Phytotoxic Substances from Schumannianthus dichotomus
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Md. Mahfuzur Rob, Kawsar Hossen, Arihiro Iwasaki, Kiyotake Suenaga, and Hisashi Kato-Noguchi
- Subjects
schumannianthus dichotomus ,phytotoxic substances ,growth inhibition ,syringic acid ,methyl syringate ,Botany ,QK1-989 - Abstract
The phytotoxic potential of plants and their constituents against other plants is being increasingly investigated as a possible alternative to synthetic herbicides to control weeds in crop fields. In this study, we explored the phytotoxicity and phytotoxic substances of Schumannianthus dichotomus, a perennial wetland shrub native to Bangladesh, India, and Myanmar. Leaf extracts of S. dichotomus exerted strong phytotoxicity against two dicot species, alfalfa and cress, and two monocot species, barnyard grass and Italian ryegrass. A bioassay-driven purification process yielded two phenolic derivatives, syringic acid and methyl syringate. Both constituents significantly inhibited the growth of cress and Italian ryegrass in a concentration-dependent manner. The concentrations required for 50% growth inhibition (I50 value) of the shoot and root growth of cress were 75.8 and 61.3 μM, respectively, for syringic acid, compared with 43.2 and 31.5 μM, respectively, for methyl syringate. Similarly, to suppress the shoot and root growth of Italian rye grass, a greater amount of syringic acid (I50 = 213.7 and 175.9 μM) was needed than methyl syringate (I50 = 140.4 to 130.8 μM). Methyl syringate showed higher phytotoxic potential than syringic acid, and cress showed higher sensitivity to both substances. This study is the first to report on the phytotoxic potential of S. dichotomus and to identify phytotoxic substances from this plant material.
- Published
- 2020
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22. Nimbolide B and Nimbic Acid B, Phytotoxic Substances in Neem Leaves with Allelopathic Activity
- Author
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Hisashi Kato-Noguchi, Md Abdus Salam, Osamu Ohno, and Kiyotake Suenaga
- Subjects
allelopathy ,Azadirachta indica ,bioactive compound ,growth inhibitor ,medicinal plant ,phytotoxicity ,Organic chemistry ,QD241-441 - Abstract
Neem (Azadirachta indica) has been widely used as a traditional medicine and several bioactive compounds have been isolated from this species, but to date no potent allelopathic active substance has been reported. Therefore, we investigated possible allelopathic property and phytotoxic substances with allelopathic activity in neem. An aqueous methanol extract of neem leaves inhibited the growth of roots and shoots of cress, lettuce, alfalfa, timothy, crabgrass, ryegrass, barnyard grass and jungle rice. The extracts were then purified by several chromatographic runs while monitoring the inhibitory activity and two phytotoxic substances were isolated. The chemical structures of the two substances were determined by spectral data to correspond to novel compounds, nimbolide B (1) and nimbic acid B (2). Nimbolide B inhibited the growth of cress and barnyard grass at concentrations greater than 0.1‒3.0 μM. Nimbic acid B inhibited the growth of cress and barnyard grass at concentrations greater than 0.3–1.0 μM. These results suggest that nimbolide B and nimbic acid B may contribute to the allelopathic effects caused by neem leaves.
- Published
- 2014
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23. Tree Fern Cyathea lepifera May Survive by Its Phytotoxic Property
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Noriyuki Ida, Arihiro Iwasaki, Toshiaki Teruya, Kiyotake Suenaga, and Hisashi Kato-Noguchi
- Subjects
cyathea lepifera ,tree fern ,growth inhibitor ,phytotoxicity ,Botany ,QK1-989 - Abstract
Cyatheaceae (tree ferns) appeared during the Jurassic period and some of the species still remain. Those species may have some morphological and/or physiological characteristics for survival. A tree fern was observed to suppress the growth of other ligneous plants in a tropical forest. It was assumed that the fern may release toxic substances into the forest floor, but those toxic substances have not yet been identified. Therefore, we investigated the phytotoxicity and phytotoxic substances of Cyathea lepifera (J. Sm. ex Hook.) Copel. An aqueous methanol extract of C. lepifera fronds inhibited the growth of roots and shoots of dicotyledonous garden cress (Lepidum sativum L.), lettuce (Lactuca sativa L.), and alfalfa (Medicago sativa L.), and monocotyledonous ryegrass (Lolium multiflorum Lam.), timothy (Phleum pratense L.), and barnyardgrass (Echinochloa crus-galli (L.) P. Beauv.). The results suggest that C. lepifera fronds may have phytotoxicity and contain some phytotoxic substances. The extract was purified through several chromatographic steps during which inhibitory activity was monitored, and p-coumaric acid and (-)-3-hydroxy-β-ionone were isolated. Those compounds showed phytotoxic activity and may contribute to the phytotoxic effects caused by the C. lepifera fronds. The fronds fall and accumulate on the forest floor through defoliation, and the compounds may be released into the forest soils through the decomposition process of the fronds. The phytotoxic activities of the compounds may be partly responsible for the fern’s survival.
- Published
- 2019
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24. Garcienone, a Novel Compound Involved in Allelopathic Activity of Garcinia Xanthochymus Hook
- Author
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Md. Mahfuzur Rob, Arihiro Iwasaki, Ryota Suzuki, Kiyotake Suenaga, and Hisashi Kato-Noguchi
- Subjects
allelopathy ,Garcinia xanthochymus ,growth inhibition ,allelopathic substances ,garcienone ,Botany ,QK1-989 - Abstract
Plants are sources of diversified allelopathic substances that can be investigated for use in eco-friendly and efficient herbicides. An aqueous methanol extract from the leaves of Garcinia xanthochymus exhibited strong inhibitory activity against barnyard grass (Echinochloa crus-galli (L.) P. Beauv.), foxtail fescue (Vulpia myuros (L.) C.C.), alfalfa (Medicago sativa L.), and cress (Lepidium sativum L.), and appears to be a promising source of allelopathic substances. Hence, bio-activity guided purification of the extract through a series of column chromatography steps yielded a novel compound assigned as garcienone ((R, E)-5-hydroxy-5-((6S, 9S)-6-methyl-9-(prop-13-en-10-yl) tetrahydrofuran-6-yl) pent-3-en-2-one). Garcienone significantly inhibited the growth of cress at a concentration of 10 μM. The concentrations resulting in 50% growth inhibition (I50) of cress roots and shoots were 120.5 and 156.3 μM, respectively. This report is the first to isolate and identify garcienone and to determine its allelopathic potential.
- Published
- 2019
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25. Marine Natural Products: A Source of Novel Anticancer Drugs
- Author
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Shaden A. M. Khalifa, Nizar Elias, Mohamed A. Farag, Lei Chen, Aamer Saeed, Mohamed-Elamir F. Hegazy, Moustafa S. Moustafa, Aida Abd El-Wahed, Saleh M. Al-Mousawi, Syed G. Musharraf, Fang-Rong Chang, Arihiro Iwasaki, Kiyotake Suenaga, Muaaz Alajlani, Ulf Göransson, and Hesham R. El-Seedi
- Subjects
marine ,plants ,microorganism ,antitumor ,anticancer ,cytotoxic ,clinical trials ,drugs ,Biology (General) ,QH301-705.5 - Abstract
Cancer remains one of the most lethal diseases worldwide. There is an urgent need for new drugs with novel modes of action and thus considerable research has been conducted for new anticancer drugs from natural sources, especially plants, microbes and marine organisms. Marine populations represent reservoirs of novel bioactive metabolites with diverse groups of chemical structures. This review highlights the impact of marine organisms, with particular emphasis on marine plants, algae, bacteria, actinomycetes, fungi, sponges and soft corals. Anti-cancer effects of marine natural products in in vitro and in vivo studies were first introduced; their activity in the prevention of tumor formation and the related compound-induced apoptosis and cytotoxicities were tackled. The possible molecular mechanisms behind the biological effects are also presented. The review highlights the diversity of marine organisms, novel chemical structures, and chemical property space. Finally, therapeutic strategies and the present use of marine-derived components, its future direction and limitations are discussed.
- Published
- 2019
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26. Allelopathic Potency and an Active Substance from Anredera cordifolia (Tenore) Steenis
- Author
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Ichsan Nurul Bari, Hisashi Kato-Noguchi, Arihiro Iwasaki, and Kiyotake Suenaga
- Subjects
Anredera cordifolia ,allelopathy ,3-hydroxy-alpha-ionone ,seedling development ,Botany ,QK1-989 - Abstract
Anredera cordifolia (Tenore) Steenis is widely planted as an ornamental and medicinal plant in Indonesia. On the other hand, in some other countries this plant is classified as a noxious weed. As a harmful weed, A. cordifolia is reported to have the ability to smother all native vegetation, collapse canopies of tall trees, cultivate as a ground cover and disrupt native seedling development. There is no available information about the involvement of any allelochemicals from A. cordifolia related to these issues. The present study evaluated the allelopathic effect by isolating and identifying the allelopathic substance from A. cordifolia leaf extract. The allelopathic potency of A. cordifolia was determined by a series of bioassays of shoot and root growth on some selected test plants. Separation and purification of the active substances was achieved through several chromatography processes. Finally, the substances with allelopathic activity were identified through high-resolution electrospray ionization mass spectrometry (HRESIMS) analysis and determined by the specific rotation of compound, proton and carbon NMR spectroscopies. The results show that A. cordifolia possesses allelopathic properties which affect other plant species. The isolated compound from the plant material, 3-hydroxy-alpha-ionone, may contribute to the allelopathic effects of A. cordifolia.
- Published
- 2019
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27. Isolation and Identification of Potent Allelopathic Substances in a Traditional Bangladeshi Rice Cultivar Kartikshail
- Author
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Hisashi Kato-Noguchi, Md Abdus Salam, and Kiyotake Suenaga
- Subjects
Allelopathy ,Bangladeshi rice ,Biological activity ,Echinochloa ,Growth inhibitor ,Plant culture ,SB1-1110 - Abstract
Aqueous methanol extracts of a traditional Bangladeshi rice cultivar (Oryza sativa L. cv. Kartikshail) inhibited root and shoot growth of cress (Lepidium sativum), lettuce (Lactuca sativa), alfalfa (Medicago sativa), timothy (Phleum pratense), crabgrass (Digitaria sanguinalis), Italian ryegrass (Lolium multiflorum), barnyardgrass (Echinochloa crus-galli) and jungle rice (Echinochloa colonum). The inhibition was increased with increasing the extract concentration, which suggests that cv. Kartikshail may have growth inhibitory substances and allelopathic potential. The aqueous methanol extract of cv. Kartikshail was purified and two main inhibitory substances were isolated and identified by spectral data as 3-hydroxy-β-ionone and 9-hydroxy-4-megastigmen-3-one. The concentrations required for 50% growth inhibition on cress roots and shoots, respectively, were 4.9 and 9.5 μM for 3-hydroxy-β-ionone, and 0.54 and 0.72 μM for 9-hydroxy-4-megastigmen-3-one. The concentrations required for 50% growth inhibition on barnyardgrass roots and shoots, respectively, were 160 and 310 μM for 3-hydroxy-β-ionone, and 53 and 140 μM for 9-hydroxy-4-megastigmen-3-one. The inhibitory activity of a mixture of the two compounds was much higher than that of the sum of the two compounds, suggesting that the two compounds may act synergistically to inhibit the growth of cress and barnyardgrass. The present research suggests that 3-hydroxy- β-ionone and 9-hydroxy-4-megastigmen-3-one may be responsible for the growth inhibitory effect of cv. Kartikshail and may play important roles in the allelopathy of cv. Kartikshail. The traditional Bangladeshi rice cultivar Kartikshail may be potentially useful for weed management as a weed suppressing agent when this rice cultivar is incorporated into the soil or included in rice-based cropping systems.
- Published
- 2011
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28. Isolation of C11 Cyclopentenones from Two Didemnid Species, Lissoclinum sp. and Diplosoma sp.
- Author
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Katsuhiro Ueda, Kiyotake Suenaga, Junsei Taira, Toshiaki Teruya, Takayuki Ogi, and Palupi Margiastuti
- Subjects
Lissoclinum ,Diplosoma ,cyclopentenone ,didemnenone ,cytotoxicity ,Biology (General) ,QH301-705.5 - Abstract
A series of new C11 cyclopentenones 1-7 was isolated, together with four known metabolites 9/10, 12 and 13, from the extract of the didemnid ascidian Lissoclinum sp. The other didemnid ascidian Diplosoma sp. contained didemnenones 1, 2 and 5, and five known metabolites 8-12. The structures of 1-7 were elucidated by spectroscopic analyses. Cytotoxicity of the isolated compounds was evaluated against three human cancer cell lines (HCT116, A431 and A549).
- Published
- 2009
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29. Three New Malyngamides from the Marine Cyanobacterium Moorea producens
- Author
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Kosuke Sueyoshi, Aki Yamano, Kaori Ozaki, Shimpei Sumimoto, Arihiro Iwasaki, Kiyotake Suenaga, and Toshiaki Teruya
- Subjects
marine cyanobacteria ,malyngamides ,glucose uptake ,AMPK ,Biology (General) ,QH301-705.5 - Abstract
Three new compounds of the malyngamide series, 6,8-di-O-acetylmalyngamide 2 (1), 6-O-acetylmalyngamide 2 (2), and N-demethyl-isomalyngamide I (3), were isolated from the marine cyanobacterium Moorea producens. Their structures were determined by spectroscopic analysis and chemical derivatization and degradation. These compounds stimulated glucose uptake in cultured L6 myotubes. In particular, 6,8-di-O-acetylmalyngamide 2 (1) showed potent activity and activated adenosine monophosphate-activated protein kinase (AMPK).
- Published
- 2017
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30. Suaveolic Acid: A Potent Phytotoxic Substance of Hyptis suaveolens
- Author
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A. K. M. Mominul Islam, Osamu Ohno, Kiyotake Suenaga, and Hisashi Kato-Noguchi
- Subjects
Technology ,Medicine ,Science - Abstract
Hyptis suaveolens (Lamiaceae) is an exotic invasive plant in many countries. Earlier studies reported that the aqueous, methanol, and aqueous methanol extract of H. suaveolens and its residues have phytotoxic properties. However, to date, the phytotoxic substances of this plant have not been reported. Therefore, the objectives of this study were isolation and identification of phytotoxic substances of H. suaveolens. Aqueous methanol extract of this plant was purified by several chromatographic runs through bioassay guided fractionation using garden cress (Lepidium sativum) as a test plant. Final purification of a phytotoxic substance was achieved by reverse phase HPLC and characterized as 14α-hydroxy-13β-abiet-8-en-18-oic acid (suaveolic acid) by high-resolution ESI-MS, 1H-,13C-NMR, CD, and specific rotation. Suaveolic acid inhibited the shoot growth of garden cress, lettuce (Lactuca sativa), Italian ryegrass (Lolium multiflorum), and barnyard grass (Echinochloa crus-galli) at concentrations greater than 30 µM. Root growth of all but lettuce was also inhibited at concentrations greater than 30 µM. The inhibitory activities were concentration dependent. Concentrations required for 50% growth inhibition of suaveolic acid for those test plant species were ranged from 76 to 1155 µM. Therefore, suaveolic acid is phytotoxic and may be responsible for the phytotoxicity of H. suaveolens plant extracts.
- Published
- 2014
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31. Isolation of Hennaminal and Isolation and Total Synthesis of Hennamide, Pyrrolinone Compounds from the Marine Cyanobacterium Rivularia sp
- Author
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Hiroki Takahashi, Arihiro Iwasaki, Akira Ebihara, Raimu Taguchi, Ghulam Jeelani, Tomoyoshi Nozaki, and Kiyotake Suenaga
- Subjects
Organic Chemistry ,Physical and Theoretical Chemistry ,Biochemistry - Published
- 2023
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32. Unique Initiation and Termination Mechanisms Involved in the Biosynthesis of a Hybrid Polyketide-Nonribosomal Peptide Lyngbyapeptin B Produced by the Marine Cyanobacterium Moorena bouillonii
- Author
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Fumitaka Kudo, Takuji Chikuma, Mizuki Nambu, Taichi Chisuga, Shimpei Sumimoto, Arihiro Iwasaki, Kiyotake Suenaga, Akimasa Miyanaga, and Tadashi Eguchi
- Subjects
Molecular Medicine ,General Medicine ,Biochemistry - Published
- 2023
33. Total Synthesis of Caldorazole, a Potent Mitochondrial Respiratory Chain Inhibitor without Chiral Centers
- Author
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Yuta Miyamoto, Arihiro Iwasaki, Haruka Fujimura, Chihiro Kudo, Naoaki Kurisawa, Osamu Ohno, and Kiyotake Suenaga
- Subjects
Organic Chemistry - Published
- 2023
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34. Isolation and Total Synthesis of Beru’amide, an Antitrypanosomal Polyketide from a Marine Cyanobacterium Okeania sp
- Author
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Raimu Taguchi, Arihiro Iwasaki, Akira Ebihara, Ghulam Jeelani, Tomoyoshi Nozaki, and Kiyotake Suenaga
- Subjects
Organic Chemistry ,Physical and Theoretical Chemistry ,Biochemistry - Published
- 2022
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35. Isolation of Caldorazole, a Thiazole-Containing Polyketide with Selective Cytotoxicity under Glucose-Restricted Conditions
- Author
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Osamu Ohno, Arihiro Iwasaki, Kyouhei Same, Chihiro Kudo, Erika Aida, Kazuya Sugiura, Shimpei Sumimoto, Toshiaki Teruya, Etsu Tashiro, Siro Simizu, Kenji Matsuno, Masaya Imoto, and Kiyotake Suenaga
- Subjects
Thiazoles ,Glucose ,Polyketides ,Organic Chemistry ,Humans ,Deoxyglucose ,Physical and Theoretical Chemistry ,Glycolysis ,Biochemistry - Abstract
Caldorazole (
- Published
- 2022
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36. Structural Determination, Total Synthesis, and Biological Activity of Iezoside, a Highly Potent Ca2+-ATPase Inhibitor from the Marine Cyanobacterium Leptochromothrix valpauliae
- Author
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Naoaki Kurisawa, Arihiro Iwasaki, Kazuya Teranuma, Shingo Dan, Chikashi Toyoshima, Masaru Hashimoto, and Kiyotake Suenaga
- Subjects
Colloid and Surface Chemistry ,General Chemistry ,Biochemistry ,Catalysis - Published
- 2022
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37. Odookeanynes A and B, Acetylene-Containing Lipopeptides from an Okeania sp. Marine Cyanobacterium
- Author
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Aki Yamano, Yuka Asato, Noriyuki Natsume, Arihiro Iwasaki, Kiyotake Suenaga, and Toshiaki Teruya
- Subjects
Pharmacology ,Complementary and alternative medicine ,Organic Chemistry ,Drug Discovery ,Pharmaceutical Science ,Molecular Medicine ,Analytical Chemistry - Published
- 2021
- Full Text
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38. First Total Synthesis and Structure–Activity Relationship of Iheyamide A, an Antitrypanosomal Linear Peptide Isolated from a Dapis sp. Marine Cyanobacterium
- Author
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William H. Gerwick, Naoaki Kurisawa, Tomoyoshi Nozaki, Kiyotake Suenaga, Kazuya Teranuma, Yulia Rahmawati, Ghulam Jeelani, and Arihiro Iwasaki
- Subjects
Pharmacology ,chemistry.chemical_classification ,Natural product ,Stereochemistry ,Antiparasitic ,medicine.drug_class ,Organic Chemistry ,Pharmaceutical Science ,Total synthesis ,Trypanosoma brucei rhodesiense ,Peptide ,Analytical Chemistry ,chemistry.chemical_compound ,Complementary and alternative medicine ,chemistry ,Drug Discovery ,medicine ,Molecular Medicine ,Potency ,Structure–activity relationship ,Moiety - Abstract
Iheyamide A (1) is an antitrypanosomal linear peptide isolated from a Dapis sp. marine cyanobacterium by our group in 2020, and based on structure-activity relationships of its natural analogues, the C-terminal pyrrolinone moiety has been identified as the phamacophore for its antiparasitic activity. Further, we isolated this pyrrolinone moiety by itself as a new natural product from the marine cyanobacterium and named it iheyanone (2). As expected, iheyanone (2) showed antitrypanosomal activity, but its potency was weaker than iheyamide A (1). To clarify more detailed structure-activity relationships, we completed a total synthesis of iheyamide A (1) along with iheyanone (2) and evaluated the antitrypanosomal activities of several synthetic intermediates. As a result, we found that the longer the peptide chain, the stronger the antitrypanosomal activity. As iheyamide A (1) showed selective toxicity against Trypanosoma brucei rhodesiense, these findings can provide design guidelines for antitrypanosomal drugs.
- Published
- 2021
- Full Text
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39. Isolation and Total Synthesis of Kinenzoline, an Antitrypanosomal Linear Depsipeptide Isolated from a Marine Salileptolyngbya sp. Cyanobacterium
- Author
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Arihiro Iwasaki, Tomoyoshi Nozaki, Kiyotake Suenaga, Naoaki Kurisawa, Keisuke Otomo, and Ghulam Jeelani
- Subjects
Depsipeptide ,chemistry.chemical_compound ,Causative organism ,chemistry ,Stereochemistry ,Organic Chemistry ,Total synthesis ,Trypanosoma brucei rhodesiense ,Highly selective ,Lead compound ,IC50 - Abstract
Kinenzoline (1), a new linear depsipeptide, was isolated from a marine Salileptolyngbya sp. cyanobacterium. Its structure was elucidated by spectroscopic analyses and degradation reactions. In addition, we achieved a total synthesis of 1 and confirmed its structure. Kinenzoline (1) showed highly selective antiproliferative activity against the causative organism of sleeping sickness, Trypanosoma brucei rhodesiense (IC50 4.5 μM), compared to normal human cells (WI-38, IC50 > 100 μM). Kinenzoline (1) is a promising lead compound for the development of new antitrypanosomal drugs.
- Published
- 2021
- Full Text
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40. Isolation and Total Synthesis of Bromoiesol sulfates, Antitrypanosomal arylethers from a Salileptolyngbya sp. Marine Cyanobacterium
- Author
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Arihiro Iwasaki, Kiyotake Suenaga, Youhei Miura, Akira Ebihara, Ghulam Jeelani, and Tomoyoshi Nozaki
- Subjects
chemistry.chemical_compound ,Hydrolysis ,chemistry ,Stereochemistry ,Aryl ,Organic Chemistry ,Total synthesis ,Trypanosoma brucei rhodesiense ,Sulfate ,Small molecule - Abstract
Bromoiesol sulfates A (1) and B (2), new polyhalogenated aryl sulfates, were isolated from a Salileptolyngbya sp. marine cyanobacterium along with their hydrolyzed compounds, bromoiesols A (3) and B (4). To pick up the candidates of their structures, we used Small Molecule Accurate Recognition Technology (SMART), an artificial intelligence-based structure-prediction tool, and their structures were elucidated on the basis of single-crystal X-ray diffraction analysis of bromoiesols (3 and 4). In addition, to verify the structures, the total synthesis of bromoiesol A sulfate (1) and bromoiesol A (3) was achieved. The bromoiesol family, especially bromoiesols (3 and 4), selectively inhibited the growth of the bloodstream form of Trypanosoma brucei rhodesiense, the causative agent of human African sleeping sickness.
- Published
- 2021
- Full Text
- View/download PDF
41. Motobamide, an Antitrypanosomal Cyclic Peptide from a Leptolyngbya sp. Marine Cyanobacterium
- Author
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Kiyotake Suenaga, Ryota Suzuki, Toshinori Sato, Ghulam Jeelani, Naoaki Kurisawa, Arihiro Iwasaki, Tomoyoshi Nozaki, Teruhiko Matsubara, and Hiroki Takahashi
- Subjects
Pharmacology ,chemistry.chemical_classification ,010405 organic chemistry ,Stereochemistry ,Organic Chemistry ,Absolute configuration ,Pharmaceutical Science ,Trypanosoma brucei rhodesiense ,01 natural sciences ,High-performance liquid chromatography ,Cyclic peptide ,0104 chemical sciences ,Analytical Chemistry ,010404 medicinal & biomolecular chemistry ,Residue (chemistry) ,Complementary and alternative medicine ,chemistry ,Drug Discovery ,Molecular Medicine ,Cytotoxicity ,IC50 ,Leptolyngbya sp - Abstract
Motobamide (1), a new cyclic peptide containing a C-prenylated cyclotryptophan residue, was isolated from a marine Leptolyngbya sp. cyanobacterium. Its planar structure was established by spectroscopic and MS/MS analyses. The absolute configuration was elucidated based on a combination of chemical degradations, chiral-phase HPLC analyses, spectroscopic analyses, and computational chemistry. Motobamide (1) moderately inhibited the growth of bloodstream forms of Trypanosoma brucei rhodesiense (IC50 2.3 µM). However, it exhibited a weaker cytotoxicity against normal human cells (IC50 55 µM).
- Published
- 2021
- Full Text
- View/download PDF
42. Potential use of Schumannianthus dichotomus waste: the phytotoxic activity of the waste and its identified compounds
- Author
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Mahfuzur Rob, Kiyotake Suenaga, Toshiaki Teruya, Arihiro Iwasaki, Hisashi Kato-Noguchi, and Kaori Ozaki
- Subjects
0106 biological sciences ,0303 health sciences ,Rapeseed ,biology ,Chemistry ,Brassica ,Lactuca ,General Medicine ,biology.organism_classification ,01 natural sciences ,Pollution ,Vulpia ,Schumannianthus dichotomus ,Lepidium sativum ,Phleum ,03 medical and health sciences ,Horticulture ,Bioherbicide ,030304 developmental biology ,010606 plant biology & botany ,Food Science - Abstract
The phytotoxic potential of the leaves and twigs of Schumannianthus dichotomus, discarded in the mat-making industry against four test plants (lettuce (Lactuca sativa L.), rapeseed (Brassica napus L.), foxtail fescue (Vulpia myuros (L.) C.C. Gmel.) and timothy (Phleum pratense L.)) was investigated and found strong phytotoxic activity. An assay-guided fractionation of S. dichotomus extarcts against cress (Lepidium sativum L.) through a series of column chromatography steps yielded two compounds, 8-(5-oxo-2,5-dihydrofuran-2-yl) octanoic acid (ODFO) and (E)-6-hydroxy-2,6-dimethylocta-2,7-dienoic acid (8-carboxylinalool). ODFO and 8-carboxylinalool showed strong phytotoxic activity against cress and timothy. The concentrations required for 50% growth inhibition (I50 value) of the seedlings of cress and timothy were 111.94-128.01 and 36.30-91.75 µM, respectively, for ODFO, but the values were much higher at 315.98-379.13 and 107.92-148.41 µM, respectively, for 8-carboxylinalool, indicating the stronger phytotoxic activity of ODFO. This study is the first to isolate ODFO and 8-carboxylinalool from S. dichotomus and their phytotoxic potential while ODFO is firstly encountered from any natural source. The growth inhibitory activity of the identified compounds may explain their role in the phytotoxic activity of S. dichotomus, which suggests the possible use of its leaves and twigs or its active constituents as natural bioherbicides.
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- 2020
- Full Text
- View/download PDF
43. Allelopathic potential and identification of two allelopathic substances in Eleocharis atropurpurea
- Author
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Farhana Zaman, Hisashi Kato-Noguchi, Kiyotake Suenaga, and Arihiro Iwasaki
- Subjects
Acetosyringone ,biology ,Pantropical ,Plant Science ,Subtropics ,biology.organism_classification ,Syringaldehyde ,chemistry.chemical_compound ,chemistry ,Botany ,Eleocharis atropurpurea ,Identification (biology) ,Weed ,Ecology, Evolution, Behavior and Systematics ,Allelopathy - Abstract
Eleocharis atropurpurea is an annual weed and distributed widely in pantropical and subtropical regions. Yet, there have been no studies on the allelopathy of E. atropurpurea. Therefore, we investi...
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- 2020
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- View/download PDF
44. Iheyamides A–C, Antitrypanosomal Linear Peptides Isolated from a Marine Dapis sp. Cyanobacterium
- Author
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Kiyotake Suenaga, Tomoyoshi Nozaki, Ghulam Jeelani, Naoaki Kurisawa, and Arihiro Iwasaki
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Pharmacology ,010405 organic chemistry ,Stereochemistry ,Chemistry ,Organic Chemistry ,Pharmaceutical Science ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,010404 medicinal & biomolecular chemistry ,Complementary and alternative medicine ,Drug Discovery ,Molecular Medicine ,Degradation (geology) - Abstract
Iheyamides A (1), B (2), and C (3), new linear peptides, were isolated from a marine Dapis sp. cyanobacterium. Their structures were elucidated by spectroscopic analyses and degradation reactions. ...
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- 2020
- Full Text
- View/download PDF
45. Irijimasides A–E, Macrolide Glycosides from an Okeania sp. Marine Cyanobacterium
- Author
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Arihiro Iwasaki, Noriyuki Natsume, Aki Yamano, Toshiaki Teruya, Kiyotake Suenaga, Miki Yamada, and Shimpei Sumimoto
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Pharmacology ,chemistry.chemical_classification ,biology ,Activator (genetics) ,Stereochemistry ,Organic Chemistry ,Acid phosphatase ,Pharmaceutical Science ,Glycoside ,Ligand (biochemistry) ,Analytical Chemistry ,chemistry.chemical_compound ,medicine.anatomical_structure ,Complementary and alternative medicine ,chemistry ,RANKL ,Osteoclast ,Drug Discovery ,biology.protein ,medicine ,Molecular Medicine ,Derivatization ,Two-dimensional nuclear magnetic resonance spectroscopy - Abstract
Irijimasides A-E (1-5), a series of new 14-membered macrolide glycosides, were isolated from a marine cyanobacterium collected in Okinawa. The gross structures of 1-5 were established by spectroscopic analysis, including 2D NMR, while absolute stereostructures were determined based on NOESY spectra, chemical derivatization, and ECD data. All five macrolides suppressed receptor activator of nuclear factor-κB ligand (RANKL)-induced tartrate-resistant acid phosphatase (TRAP) activity in mouse RAW264 macrophage cells, indicating that these compounds inhibit osteoclast formation.
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- 2020
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46. Isolation and identification of two phytotoxic compounds from the medicinal plant Cassia alata L
- Author
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Kiyotake Suenaga, Krishna Rany Das, Keitaro Iwasaki, and Hisashi Kato-Noguchi
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biology ,Traditional medicine ,Cassia ,Rhododendrol ,Phytotoxicity ,Identification (biology) ,biology.organism_classification ,Isolation (microbiology) ,Agronomy and Crop Science - Published
- 2020
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47. Ikoamide, an Antimalarial Lipopeptide from an Okeania sp. Marine Cyanobacterium
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Tomoyoshi Nozaki, Teruhiko Matsubara, Keitaro Iwasaki, Yumiko Saito-Nakano, Shimpei Sumimoto, Arihiro Iwasaki, Kiyotake Suenaga, and Toshinori Sato
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Pharmacology ,Chromatography ,010405 organic chemistry ,Organic Chemistry ,Absolute configuration ,Pharmaceutical Science ,Lipopeptide ,01 natural sciences ,High-performance liquid chromatography ,0104 chemical sciences ,Analytical Chemistry ,010404 medicinal & biomolecular chemistry ,chemistry.chemical_compound ,Complementary and alternative medicine ,chemistry ,Drug Discovery ,Molecular Medicine ,Derivatization ,Cytotoxicity ,IC50 ,Human cancer - Abstract
An antimalarial lipopeptide, ikoamide, was isolated from an Okeania sp. marine cyanobacterium. Its gross structure was established by spectroscopic analyses, and the absolute configuration was clarified based on a combination of chiral-phase HPLC analyses, spectroscopic analyses, and derivatization reactions. Ikoamide showed strong antimalarial activity with an IC50 value of 0.14 μM without cytotoxicity against human cancer cell lines at 10 μM.
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- 2020
- Full Text
- View/download PDF
48. First Total Synthesis and Structure-Activity Relationship of Iheyamide A, an Antitrypanosomal Linear Peptide Isolated from a
- Author
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Arihiro, Iwasaki, Kazuya, Teranuma, Naoaki, Kurisawa, Yulia, Rahmawati, Ghulam, Jeelani, Tomoyoshi, Nozaki, William H, Gerwick, and Kiyotake, Suenaga
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Aquatic Organisms ,Structure-Activity Relationship ,Japan ,Molecular Structure ,Trypanosoma brucei brucei ,Cyanobacteria ,Peptides ,Trypanocidal Agents - Abstract
Iheyamide A (
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- 2021
49. Isolation and Total Synthesis of Bromoiesol sulfates, Antitrypanosomal arylethers from a
- Author
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Akira, Ebihara, Arihiro, Iwasaki, Youhei, Miura, Ghulam, Jeelani, Tomoyoshi, Nozaki, and Kiyotake, Suenaga
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Trypanosoma brucei rhodesiense ,Trypanosomiasis, African ,Artificial Intelligence ,Sulfates ,Antiprotozoal Agents ,Animals ,Humans - Abstract
Bromoiesol sulfates A (
- Published
- 2021
50. Isolation and Total Synthesis of Kinenzoline, an Antitrypanosomal Linear Depsipeptide Isolated from a Marine
- Author
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Naoaki, Kurisawa, Keisuke, Otomo, Arihiro, Iwasaki, Ghulam, Jeelani, Tomoyoshi, Nozaki, and Kiyotake, Suenaga
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Trypanosomiasis, African ,Depsipeptides ,Antiprotozoal Agents ,Animals ,Humans ,Cyanobacteria ,Trypanocidal Agents - Abstract
Kinenzoline (
- Published
- 2021
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