42 results on '"Kleiman, F."'
Search Results
2. Understanding civil servants' intentions to open data: factors influencing behavior to disclose data
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Kleiman, F. (author), Jansen, S.J.T. (author), Meijer, Sebastiaan (author), Janssen, M.F.W.H.A. (author), Kleiman, F. (author), Jansen, S.J.T. (author), Meijer, Sebastiaan (author), and Janssen, M.F.W.H.A. (author)
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Purpose: The opening of government data is high on the policy agenda of governments worldwide. However, data release faces barriers due to limited support of civil servants, whereas the literature neglects civil servants' role in opening data. This paper aims at understanding why civil servants can be reluctant to support the disclosure of data. The authors developed a model to explain civil servants' behavioral intention to open data. Design/methodology/approach: The authors test a series of hypotheses by collecting and analyzing survey data from 387 civil servants and by applying multivariate hierarchical regression. Findings: The results indicate the factors influencing the behavior of civil servants. Social influences, performance expectancy, data management knowledge and risks have a significant influence. Personal characteristics control these effects. Research limitations/implications: Caution is needed to generalize the findings towards the support to open data provision by civil servants. Though the analyzed sample was limited to Brazil, other countries and cultures might yield different outcomes. Larger and more diversified samples might indicate significant effects on variables not found in this research. Practical implications: The insights can be used to develop policies for increasing the support of civil servants towards governmental data disclosure. Originality/value: This study suggests factors of influence to civil servants' behavior intentions to disclose governmental data. It results in a model of factors, specifically for their behavioral intention at the individual level., Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., Transport and Logistics, Information and Communication Technology, Real Estate Management, Engineering, Systems and Services
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- 2023
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3. New ways for monitoring the public workforce after the pandemic: The management and performance program in the Brazilian Federal Government
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Kleiman, F. (author), Rego, Roberto Seara Mac Hado Pojo (author), Farias, Rogerio De Souza (author), Nogueira, Nathália Junca (author), Brito, Thais Barral De Oliveira (author), Marasca Bertazzi, Danilo Marasca (author), Barbosa, Marcelo Mendes (author), Kleiman, F. (author), Rego, Roberto Seara Mac Hado Pojo (author), Farias, Rogerio De Souza (author), Nogueira, Nathália Junca (author), Brito, Thais Barral De Oliveira (author), Marasca Bertazzi, Danilo Marasca (author), and Barbosa, Marcelo Mendes (author)
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The sanitary emergency of the COVID-19 pandemic resulted in a push for the adoption of teleworking in both public and private sector. In the Brazilian Federal Government, such effort was supported by a national program named Management and Performance Program - PGD (Programa de Gestão e Desempenho). The PGD enabled the country's federal civil servants from the Executive to work from home and was established with three main pillars. First, it supported new ways of contracting deliverables between management and teams. They allowed work to be monitored remotely instead of the old-fashioned procedure of controlling time sheets. Second, to unleash the possibilities of such contracting, governmental units were pushed to map their main service delivery processes and transform them into tasks that could be rationally distributed to teams. Third, new digital systems registered these agreements and monitored their execution monthly. Based on the case study of the PGD, this policy paper describes the development of the PGD within the Brazilian government and indicates challenges to progress with its implementation. We conclude that the PGD might have created the needed structure for the government to step towards new ways of managing the workforce. This policy paper presented the case study of the PGD, a transformation program within the Brazilian government that enabled remote work in public service in Brazil. The PGD study case was based on documents, websites, articles, and news from the Program's implementation process. Many of the presented discussions are ongoing and aim to support practical and academic discussions on the challenges of implementing government transformation. The PGD is the Brazilian national Program that comprises legislation, digital systems, and management practices improved during the pandemic to enable teleworking in the government. Besides remote work, it can be seen as a driver for setting new work planning and monitoring practices. The PGD deman, Information and Communication Technology
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- 2023
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4. How do personality traits influence Open Government Data (OGD) adoption and usage? Investigating the indirect and moderating effects
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Rizun, Nina (author), Alexopoulos, Charalampos (author), Saxena, Stuti (author), Kleiman, F. (author), Matheus, R. (author), Rizun, Nina (author), Alexopoulos, Charalampos (author), Saxena, Stuti (author), Kleiman, F. (author), and Matheus, R. (author)
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Open Government Data (OGD) research has focused for a long on the adoption and usage from the perspectives of users across different contexts. The underlying rationale for this specific focus is that OGD initiatives are undertaken to further citizen engagement with OGD for value generation and innovation purposes. Conceding that usage propensity is different across individuals, it is important to understand the influence of personality traits vis-à-vis OGD adoption and usage. Given that OGD has been regarded as a sophisticated "technology"and the role of personality traits has been considered as important in the adoption and usage of "technologies"in general, therefore, the present study contributes to the extant OGD-focused literature from a novel dimension. The study invokes the adapted model of the Unified Theory of Technology Adoption and Use (UTAUT) alongside the HEXACO-100 inventory constructs for studying the relationships between the constructs with a sample of 530 respondents. The results demonstrate that higher user Openness to Experience contributes to their higher Effort and Performance Expectancy; exposure to Social Influence; an increased level of Trust; and a more positive perception of Facilitating Conditions and Information Quality. Agreeable people are more likely to voluntarily use OGD. An individual's conscientiousness improves their perception of factors related to OGD quality. Excessive emotionality leads to a more critical perception of systems and information quality issues. Our findings also attest to the moderating impact of Honesty-Humility across Information Quality-Behavioral Intention positively; Extraversion across Information Quality-Behavioral Intention negatively and Extraversion across Trust-Behavioral Intention positively. Honesty turns out to be important for considering Information Quality vis-à-vis OGD adoption and usage but whilst extroverts are concerned about Information Quality, i.e. flawless information retrieval via OGD s, Information and Communication Technology
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- 2023
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5. Serious Games for Building Data Capacity
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Di Staso, D. (author), Mulder, I. (author), Janssen, M.F.W.H.A. (author), Kleiman, F. (author), Di Staso, D. (author), Mulder, I. (author), Janssen, M.F.W.H.A. (author), and Kleiman, F. (author)
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Open data can support the creation of new services, facilitate research, and provide insights into everyday issues affecting citizens. Although public administrations are making efforts to create sustainable and inclusive open data systems, there is limited capacity to identify suitable datasets, clean, release, and reuse them. Serious games offer a possible solution for data capacity building and have already been used to train civil servants and citizens on the topic of open data. This research presents a review of serious games and discusses their potential for data capacity building. The games selected in the review are classified and described according to their different learning outcomes, formats, and type of media. Most serious games found in this review can be categorized as teaching games and are designed to raise data awareness, which is only a limited aspect of building data capacity. We found a lack of design games, research games, and policy games. Given their success for ideation in other fields, design games offer a particular opportunity to build data capacity by generating new ideas about how to reuse open datasets., Design Conceptualization and Communication, Information and Communication Technology
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- 2022
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6. A Systematic Literature Review on the Use of Games for Attitude Change: Searching for Factors Influencing Civil Servants' Attitudes
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Kleiman, F. (author), Meijer, Sebastiaan (author), Janssen, M.F.W.H.A. (author), Kleiman, F. (author), Meijer, Sebastiaan (author), and Janssen, M.F.W.H.A. (author)
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Governments are increasingly using games for civic engagement, decision making, and education. Serious gaming is a type of game that has often been advocated as a means for changing the attitude of its players and can be used for changing the attitude of civil servants. However, the relationship between games and attitude change in civil servants remains unexplored. This paper aims at identifying factors leading to attitude change of civil servants. As hardly any paper is focused on civil servants' attitude change through games, the authors broaden their research to attitude change through games in general. Out of 483 documents, 19 reference papers were analyzed in detail. Eighty-one games were found, and more than 13 different theories were identified containing 30 different influencing factors, which were found mostly to be unrelated and context-dependent. The conceptual dispersion between studies indicates that the resulting overview of factors is a first step towards creating a uniform theory. The results can help governments to design better games., Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., Transport and Logistics, Information and Communication Technology, Engineering, Systems and Services
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- 2022
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7. Changer le comportement des agents publics face à l’ouverture des données publiques: évaluation de l’effet d’un jeu par comparaison des intentions avant et après l’intervention
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Kleiman, F. (author), Janssen, M.F.W.H.A. (author), Meijer, Sebastiaan (author), Kleiman, F. (author), Janssen, M.F.W.H.A. (author), and Meijer, Sebastiaan (author)
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Les administrations adoptent de plus en plus des politiques d’ouverture des données. Toutefois, les agents publics jugent difficile de respecter ces nouvelles politiques. Les jeux peuvent les aider à pratiquer l’ouverture des données et à modifier leur comportement en faveur d’une plus grande ouverture. Dans cet article, nous évaluons l’effet d’un jeu dans le cadre d’une expérimentation qui compare, avant et après l’intervention, plusieurs facteurs ayant une incidence sur l’ouverture des données. Les bénéfices observés sont inattendus à plusieurs égards. Le jeu a permis de partager des connaissances dans les domaines de la gestion, de la confidentialité et de la sécurité des données, de modifier de manière significative les bénéfices perçus, et de faire évoluer de manière positive l’intention de comportement. Remarques à l’intention des praticiens Le comportement des agents publics a une incidence sur la façon dont les politiques publiques sont mises en œuvre. Pour beaucoup, la diffusion de données publiques par les administrations est fondamentale pour accroître la transparence publique et la participation des citoyens, et pour ouvrir de nouvelles perspectives économiques. Les jeux peuvent influer sur l’attitude des agents publics, et donc modifier les décisions des administrations. Transmettre des connaissances et fournir des enseignements tirés de nouvelles expériences peut influencer l’attitude des agents publics à l’égard des données ouvertes. Les administrations peuvent également utiliser les jeux à cette fin., Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., Transport and Logistics, Information and Communication Technology, Engineering, Systems and Services
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- 2022
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8. Real-time PCR strategy for rapid discrimination among main lymnaeid species from Argentina
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Duffy, T., Kleiman, F., Pietrokovsky, S., Issia, L., Schijman, A.G., and Wisnivesky-Colli, C.
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- 2009
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9. p53 inhibits mRNA 3′ processing through its interaction with the CstF/BARD1 complex
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Nazeer, F I, Devany, E, Mohammed, S, Fonseca, D, Akukwe, B, Taveras, C, and Kleiman, F E
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- 2011
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10. Evaluation of a Pilot Game to Change Civil Servants’ Willingness Towards Open Data Policy Making
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Kleiman, F., Janssen, M.F.W.H.A., Meijer, Sebastiaan, Wardaszko, Marcin, Lukosch, Heide, Kanegae, Hidehiko, Kriz, Willy Christian, and Grzybowska-Brzezińska, Mariola
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Open government ,Design ,business.industry ,Quasi-experiment ,media_common.quotation_subject ,Open data ,Game ,Public relations ,Civil servants ,Domain (software engineering) ,Intervention (law) ,Perception ,Bureaucracy ,business ,Survey ,media_common - Abstract
The adoption of open data policy-making by governments is limited due to different types of constraints. Civil servants are reluctant to open their data to the public for many reasons. The lack of knowledge of benefits that can be produced by the release of data and the overestimation of risks and operational complexity seems to decrease their willingness to support the opening of data. The idea that a serious game intervention can change awareness of participants in different domains is already known. Yet, games are domain dependent and concepts differ per domain. A game has never been used for the emerging domain of open data in which civil servants are operating in a bureaucratic environment having a risk-averse culture and strict institutional rules. A role-playing game prototype was designed for civil servants to experience open data policy-making. This paper analyses its first results aiming at changes of perception for the participants of the game and aims to understand the changes in behavior of civil servants that played it. For some participants, the game influenced their attitude, whereas others were not influenced. Suggesting that different approaches might be necessary for changing the attitude of different groups.
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- 2021
11. Changing Civil Servants' Awareness about Open Data Using a Collaborative Digital Game
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Di Staso, D., Kleiman, F., Crompvoets, Joep, Janssen, M.F.W.H.A., Lee, Jooho, Pereira, Gabriela Viale, and Hwang, Sungsoo
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Open government ,Government ,serious games ,Point (typography) ,business.industry ,05 social sciences ,collaborative learning ,050301 education ,open data ,Collaborative learning ,02 engineering and technology ,Public relations ,Transparency (behavior) ,Civil servants ,Open data ,covid-19 ,open data policy ,020204 information systems ,Accountability ,0202 electrical engineering, electronic engineering, information engineering ,Business ,gaming ,0503 education - Abstract
According to open government principles, public administration should make its data available to the public to create transparency, accountability, and to facilitate participation in public decision-making. Open-Data Policies (ODPs) were developed to encourage the opening of governmental data to the public, but require collaboration and awareness from all levels of government. Gaming has proved to be successful for creating awareness. Yet the pandemic hinders in-person gameplay, and there is a need for an online game that can be played remotely. This research uses an in-person game as the starting point to develop an online collaborative digital game. The feature and design choices of the digital game are described, along with its main differences when compared to the in-person one. The essential features include interaction and discussion about open data among players, the assignment of different roles, and points awarded when making the right decision to open, partially share, or close a dataset.
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- 2021
12. A Digital Game to Learn About Open Data
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Di Staso, D., Kleiman, F., Crompvoets, Joep, Janssen, M.F.W.H.A., Scholl, Hans Jochen, Gil-Garcia, J. Ramon, Janssen, Marijn, Kalampokis, Evangelos, Lindgren, Ida, and Rodríguez Bolívar, Manuel Pedro
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Serious games ,Government ,business.industry ,media_common.quotation_subject ,Open data ,Public sector ,ComputingMilieux_PERSONALCOMPUTING ,Public office ,Sample (statistics) ,Likert scale ,Gaming ,Perception ,Open data policy ,Professional environment ,Marketing ,Covid-19 ,business ,media_common - Abstract
The implementation of open data policies requires the efforts of many public employees across different levels of government, who may be unaware of the benefits and risks of open data. Serious games have demonstrated potential for training in a professional environment. For this research, a collaborative digital serious game about open data was developed. A sample of 24 civil servants played the game. Pre-test and post-test surveys were used to evaluate the effects of the game on participants’ perception of open data. Likert score changes between pre-test and post-test indicated that the game had a positive effect on the willingness to share public sector data. By simulating the setting of a public office and by having players make decisions about whether to open certain datasets, the game facilitated learning about the benefits and disadvantages of opening data.
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- 2021
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13. WINNING DATA: Designing and testing a game to change civil servants' attitudes towards open governmental data provision
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Kleiman, F., Janssen, M.F.W.H.A., Meijer, S.A., and Delft University of Technology
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Serious Gaming ,Open Government ,Attitude ,Game Design ,Open data ,OGD ,Attitude change ,Serious Game - Abstract
Data is needed for a government to function, and civil servants generate data that can be opened. However, this data is not always publicly available. Governments open their data to meet societal needs to increase transparency, accountability, stimulate participation and innovation. The opening of governmental data can be seen as a source of uncertainty for public servants, or it can even be legally prohibited, depending on how the regulation is interpreted. For instance, open data might be experienced as a burden or not easy to practice, whereas the opening might create societal relevance. This research focuses on overcoming behavioral barriers for civil servants to manage data release at the individual level by using a serious game. Open data relates to any data produced by any device or person, which is publicly shared for free or at a minimal cost, and that can be accessed by anyone. These behavioral barriers for civil servants influence governments’ decisions to make data available to the public. Behavioral barriers are the impediments for governments to release open data which originates from human behaviors. The literature suggests that behaviors are difficult to measure, and therefore, we focus on attitudes, which are measurable through declared perception. Attitude refers to a set of beliefs and feelings which is a common predictor of behavior. In this research, we use governmental civil servants’ behavioral intention to support open data to measure attitudes and the change in behavior intentions of civil servants as a proxy to analyze attitude change. Serious games are game-based interventions designed for other goals than (only) entertaining the players. They offer a safe and controlled environment for experimentation and experiential learning. The research objective of this thesis is to develop and test a game to influence the attitudes of civil servants towards the release of open data by governments, by enabling them to experience the positive and negative sides of open data in the game. Design science research was used for prototyping development and testing a game in a quasi-experimental set-up. Four research questions guided the study: RQ1. What are the behavioral barriers for civil servants to support the opening of governmental data? RQ2. What are the requirements to design a game to change civil servants’ attitudes towards supporting the opening of governmental data? RQ3. Which game design mechanisms enable the change of civil servants’ attitudes towards opening governmental data? RQ4. What are the effects of the open data game on civil servant’s attitudes towards supporting the opening of data? Each research question demanded the application of specific research methods. As the first step, systematic literature reviews were performed in the field of 1) open data provision behavioral barriers, 2) games for civil servants, 3) games for open data, and 4) games designed for attitude change. The first literature review was used to answer RQ1, whereas the other aimed at RQ2. For RQ1 (What are the behavioral barriers for civil servants to support the opening of governmental data?), the literature review identified a list of 38 behavioral barriers for civil servants influencing the opening of data. These behavioral barriers discussed in this thesis should be considered to change civil servants’ attitudes to support the opening of governmental data. For RQ2 (What are the requirements to design a game to change civil servants’ attitudes towards supporting the opening of governmental data?), three literature reviews were conducted to find game design requirements from previous research. They targeted at specific aspects of proven serious games: 1) for civil servants, to better understand the audience characteristics which could influence gameplay; 2) using open data content to inspire metaphors and operational representation of data release in the game; and 3) to change attitudes of players, targeting at successful use of game use towards attitude change. For civil servants, many games exist, whereas, for open data provision, no games were found. Even though many mechanisms exist in the literature, they did not prescribe an operationalization for an open data game. To evolve towards the most suitable game, we followed an iterative process to better understand how the game could be realized. Games are context-dependent, particularly to our specific case, open data governmental provision. Likewise, the iterative process enabled testing the operationalization of such requirements into game mechanisms. Four prototypes resulted from this game design process. Each designed prototype was evaluated, updating the lists of requirements and mechanisms for the final version of the game. •Prototype 1: Cards for open data debriefing showed that engaging mechanics could help connecting players to the open data challenges, but a card game resulted in lower levels of knowledge transfer about open data; •Prototype 2. Solvd, a group debate play-setting, resulted in interactive content from group interactions. However, the game was not entertaining, resulting in a loss of engagement; •Prototype 3. Job-matching simulator, a decision-making labor-market digital game, helped to map the real-life public service data production and use routines. This prototype highlighted the need to represent situations encountered by public servants in reality, including risks and ways to prevent them; and •Prototype 4. Open data office, a role-playing game aimed at engagement and learning for attitude change. Still, it lacked a more precise metaphor for routines and the office environment. Likewise, playing roles with humans was found to be important for our learning goals, in addition to adjusting the number of players and rounds. The prototypes resulted in the following main requirements on a serious game to influence civil servants support to the opening of data: •Requirement 1. Open government data content used in the game should be highlighted; •Requirement 2. The focus should be on a game experience that enables experiential learning; •Requirement 3. Civil servants’ practical knowledge should be reflected in the game; •Requirement 4. The game should be used as a safe environment for experimentation; •Requirement 5. The game setting should be realistic; •Requirement 6. Game dynamics should be organized as a role-playing game; and •Requirement 7. The number of roles, players, and rounds should be limited. Additionally, the literature findings combined with the outcomes of the iterative design cycles, pilot-testing, and debriefing, enabled the answering of RQ3 (Which game design mechanisms enable the change of civil servants’ attitudes towards opening governmental data?). The final version of the game, named WINNING DATA, operationalized the requirements into mechanisms that enabled players to change their attitudes towards open data. These mechanisms emerged from the design process, where each prototype debriefing informed the next round of iteration and new prototype. For instance, the needs for open data content and realism are represented through assets such as forms, files, and demand cards; demand cards express pre-defined routines: service requests. Demands are identified by specific card codes, which enable an automatic scoring system for the game facilitation; the service delivery, processed by rolling sets of dice, results in the creation of datasets. Depending on the dice combinations, privacy and security crises can occur, affecting the challenges of the game. The following final list of mechanisms resulted from this process: •Mechanism 1: Dataset description and labeling; •Mechanism 2: Card codes; •Mechanism 3: Pre-defined demands (not random); •Mechanism 4: Forms, Files and Demand cards; •Mechanism 5: Service delivery goal; •Mechanism 6: Upgrades; •Mechanism 7: Facilitation; •Mechanism 8: Crisis board; •Mechanism 9: Dice as processing machine; •Mechanism 10: Multi-player (with different roles); and •Mechanism 11: Time-limited rounds. Based on these requirements and mechanisms, WINNING DATA was designed as a four-player role-playing in-person game that can be played in a two-hour session. The game was evaluated for its effects on the attitudes of civil servants towards supporting the opening of governmental data. Playing the game consists of five rounds in which participants switch roles. The roles are citizen, two civil servants, and a manager. The player, playing the role of a citizen, demands services to the one playing the role of a civil servant; the player playing the role of civil servant has to work together with the colleague and boss to deliver the service back. Each service delivered results in a dataset which is discussed by the team and labeled by the boss. Labeling decisions influence the chances of having a privacy or security crisis in the coming rounds, resulting from specific dice combinations. Lastly, game play, data collection, and statistical analysis were used to answer the RQ4 (What are the effects of the open data game on civil servant’s attitudes towards supporting the opening of data?). Our main hypothesis is that the attitudes of civil servants can be changed by using a serious game. From the list of behavior barriers (RQ1), an initial list of factors influencing civil servants’ attitudes emerged. Four influencing factors were defined to influence Behavioral Intention, the dependent variable representing civil servants’ attitudes: lack of knowledge, performance expectancy, effort expectancy, and social influence. Explorative testing was conducted to determine which factors are at work and how the game affected them. The factors were hypothesized for testing game effects on civil servants’ attitudes to supporting open data. All factors were measured using a 33-item 7-point Likert scale questionnaire. The survey was used to measure the players’ attitudes before and after the game was played. Comparison enabled the assessment of the effects of change in their attitudes. In a quasi-experimental set-up, 77 civil servants played the game and filled in the pre- and post-test survey. Another 35 civil servants filled in the survey on two different occasions, without the gaming intervention, as a control group. The data was analyzed. Firstly, the internal reliability of the factors was checked, followed by explorative testing on the factors that did not load. The resulting factors were organized into a model which included Behavioral Intention as the dependent factor, measuring multiple dimensions of civil servants’ attitudes towards open data. Other seven factors were defined: Data Management Knowledge (DK), Performance Expectancy (PE), Risks (RK), Social Influence (SI), Knowledge of Data Production (DP), Data Sharing Knowledge (DS), and Data Costs (DC). The eight resulting hypotheses were tested using the 112 completed surveys: Hypothesis 1: Behavioral intention increases after playing the game; Hypothesis 2: The game results in more knowledge about ways to open data; Hypothesis 3: The game results in a better understanding of the expected benefits of opening data; Hypothesis 4: The game decreases expectations of the risks related to making data available; Hypothesis 5: The game reduces civil servants’ perceptions of open data practice difficulties, as exerted by hierarchies and legal frameworks; Hypothesis 6: The game increases civil servants’ knowledge of data production; Hypothesis 7: The game increases civil servants’ knowledge of the possibility of sharing data; and Hypothesis 8: The game increases civil servants’ perception of data provision costs. Through a Wilcoxon Signed Rank test, we assessed the main hypothesis and concluded that the game is likely to have a statistically significant effect on the dependent variable of Behavior Intention. As we did not find significant effects on behavior intention in the control group, our conclusion that civil servants who played the game are likely to have their attitudes towards open data increased by the game was strengthened. After that, the WINNING DATA’s gameplay additional seven hypotheses were tested. The game had a significant positive effect on Risks and Performance Expectancy. Though there were differences in the pre- and post-test scores of Data management knowledge, Social Influence, Knowledge of Data Production, Data Sharing Knowledge, and Data Costs, none of them were statistically significant. Our research has limitations resulting from (1) the limited number of participants and their distributions’ characteristics; (2) the absence of alternative strategies to which our results could be compared; and (3) the feasibility of more complex statistical analyses that were limited due to the available sample. Furthermore, this research (4) could not explore other diverse outcomes, such as a more complex model discussion on the factors influencing civil servants’ attitudes to support the opening of governmental data, which is needed and still to be done. Additionally, these limitations shed light on other improvements for new versions of the game. Future research is recommended to test the game with larger samples, players having a more diverse background, and coming from different countries. Using the same survey questions to different passive interventions, such as text and lectures, can also contribute to comparing the results. The long-term effects of the game were not investigated and recommended as a further research direction. Another further research direction is the digitalization of the game. Particularly in the light of the recent crisis of COVID-19, this is needed as playing the game with many persons in one room is not a good option. Likewise, advancing with the model discussions, including more open data elements, and extending the topics to other fields is also recommended by this thesis. Concluding, the game developed and tested during this project has proven its effects on changing civil servants’ attitudes towards the opening of governmental data. This thesis’s results can be used to design better interventions to make more governmental data available to the public.
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- 2021
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14. Behavioral factors influencing the opening of government data by civil servants
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Kleiman, F., Meijer, Sebastiaan, Janssen, M.F.W.H.A., Charalabidis, Yannis, Cunha, Maria Alexandra, and Sarantis, Demetrios
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Open Data Policy ,Related factors ,Open government ,Government ,Decision-Making ,business.industry ,media_common.quotation_subject ,05 social sciences ,02 engineering and technology ,Public relations ,Civil servants ,Open data ,Behavioral Barriers ,Incentive ,020204 information systems ,Political science ,0202 electrical engineering, electronic engineering, information engineering ,Quality (business) ,0509 other social sciences ,Listing (finance) ,050904 information & library sciences ,business ,media_common - Abstract
The actual opening of government data is done by civil servants operating within their institutional environment. As such, the institutional environment and their behavior towards the opening of data is key to increase the quality and quantity of open data. However, few studies have analyzed the behavior of government professionals towards open data policy. The objective of this paper is to review the existing literature to find the factors that influences civil servants behaviors towards open data. To identify the drivers and barriers, a literature review was performed listing the most cited papers with "open government data"and "barriers"focusing specific at the behavioral related factors. Even with the increasing of research on the topic of open data, still most of the papers focus on user drivers and barriers rather than on provider challenges. Even less studies focus on the civil servants' individual level of factors influencing their support to the release of governmental data. Most barriers found in the literature are related to infrastructural or technical issues. Whereas some individual level behavioral barriers could be found, including culture, lack of individual incentives and misunderstanding the impact of opening data, broader discussions on social norms, lack of education and experience are still missing in the literature. This paper contributes to the need for deeper understanding of the behavioral factors that influences the civil servants to support the opening of data.
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- 2020
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15. Evaluation of a Pilot Game to Change Civil Servants’ Willingness Towards Open Data Policy Making
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Kleiman, F. (author), Janssen, M.F.W.H.A. (author), Meijer, Sebastiaan (author), Kleiman, F. (author), Janssen, M.F.W.H.A. (author), and Meijer, Sebastiaan (author)
- Abstract
The adoption of open data policy-making by governments is limited due to different types of constraints. Civil servants are reluctant to open their data to the public for many reasons. The lack of knowledge of benefits that can be produced by the release of data and the overestimation of risks and operational complexity seems to decrease their willingness to support the opening of data. The idea that a serious game intervention can change awareness of participants in different domains is already known. Yet, games are domain dependent and concepts differ per domain. A game has never been used for the emerging domain of open data in which civil servants are operating in a bureaucratic environment having a risk-averse culture and strict institutional rules. A role-playing game prototype was designed for civil servants to experience open data policy-making. This paper analyses its first results aiming at changes of perception for the participants of the game and aims to understand the changes in behavior of civil servants that played it. For some participants, the game influenced their attitude, whereas others were not influenced. Suggesting that different approaches might be necessary for changing the attitude of different groups., Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., Information and Communication Technology
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- 2021
- Full Text
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16. Changing Civil Servants' Awareness about Open Data Using a Collaborative Digital Game
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Di Staso, D. (author), Kleiman, F. (author), Crompvoets, Joep (author), Janssen, M.F.W.H.A. (author), Di Staso, D. (author), Kleiman, F. (author), Crompvoets, Joep (author), and Janssen, M.F.W.H.A. (author)
- Abstract
According to open government principles, public administration should make its data available to the public to create transparency, accountability, and to facilitate participation in public decision-making. Open-Data Policies (ODPs) were developed to encourage the opening of governmental data to the public, but require collaboration and awareness from all levels of government. Gaming has proved to be successful for creating awareness. Yet the pandemic hinders in-person gameplay, and there is a need for an online game that can be played remotely. This research uses an in-person game as the starting point to develop an online collaborative digital game. The feature and design choices of the digital game are described, along with its main differences when compared to the in-person one. The essential features include interaction and discussion about open data among players, the assignment of different roles, and points awarded when making the right decision to open, partially share, or close a dataset., Information and Communication Technology
- Published
- 2021
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17. A Digital Game to Learn About Open Data
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Di Staso, D. (author), Kleiman, F. (author), Crompvoets, Joep (author), Janssen, M.F.W.H.A. (author), Di Staso, D. (author), Kleiman, F. (author), Crompvoets, Joep (author), and Janssen, M.F.W.H.A. (author)
- Abstract
The implementation of open data policies requires the efforts of many public employees across different levels of government, who may be unaware of the benefits and risks of open data. Serious games have demonstrated potential for training in a professional environment. For this research, a collaborative digital serious game about open data was developed. A sample of 24 civil servants played the game. Pre-test and post-test surveys were used to evaluate the effects of the game on participants’ perception of open data. Likert score changes between pre-test and post-test indicated that the game had a positive effect on the willingness to share public sector data. By simulating the setting of a public office and by having players make decisions about whether to open certain datasets, the game facilitated learning about the benefits and disadvantages of opening data., Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., Information and Communication Technology
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- 2021
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18. Winning Data: Designing and testing a game to change civil servants' attitudes towards open governmental data provision
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Kleiman, F. (author) and Kleiman, F. (author)
- Abstract
Data is needed for a government to function, and civil servants generate data that can be opened. However, this data is not always publicly available. Governments open their data to meet societal needs to increase transparency, accountability, stimulate participation and innovation. The opening of governmental data can be seen as a source of uncertainty for public servants, or it can even be legally prohibited, depending on how the regulation is interpreted. For instance, open data might be experienced as a burden or not easy to practice, whereas the opening might create societal relevance. This research focuses on overcoming behavioral barriers for civil servants to manage data release at the individual level by using a serious game. Open data relates to any data produced by any device or person, which is publicly shared for free or at a minimal cost, and that can be accessed by anyone. These behavioral barriers for civil servants influence governments’ decisions to make data available to the public. Behavioral barriers are the impediments for governments to release open data which originates from human behaviors. The literature suggests that behaviors are difficult to measure, and therefore, we focus on attitudes, which are measurable through declared perception. Attitude refers to a set of beliefs and feelings which is a common predictor of behavior. In this research, we use governmental civil servants’ behavioral intention to support open data to measure attitudes and the change in behavior intentions of civil servants as a proxy to analyze attitude change. Serious games are game-based interventions designed for other goals than (only) entertaining the players. They offer a safe and controlled environment for experimentation and experiential learning. The research objective of this thesis is to develop and test a game to influence the attitudes of civil servants towards the release of open data by governments, by enabling them to experience the positive and nega, Information and Communication Technology
- Published
- 2021
19. Gaming for Meaningful Interactions in Teleworking Lessons Learned during the COVID-19 Pandemic from Integrating Gaming in Virtual Meetings
- Author
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Kleiman, F. (author), Meijer, S.A. (author), Janssen, M.F.W.H.A. (author), Kleiman, F. (author), Meijer, S.A. (author), and Janssen, M.F.W.H.A. (author)
- Abstract
The recent need for social-distancing caused by COVID-19 resulted in working remotely, which can cause loneliness and disconnect from the organization. The pandemic stimulated people to look for novel ways to interact and, at the same time, socialize with each other. The change to home office increased the number of people using digital video calls; however, these impose restrictions on social bonding. In this commentary, we argue that the social bonding capacity of video calls can be increased by using games. Playing games can create a common object with shared goals, which can give players the feeling of being in the same environment, belonging to the same organization, working on joint objectives, and give an enjoyable experience. All work activities will unlikely return to the office after the pandemic is over, and preparing for remote working and socialization is needed. We recommend several avenues for research, including researching the concepts of online socialization and evaluating the effectiveness of gaming., Information and Communication Technology, Organisation and Governance
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- 2020
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20. A systematic literature review on the use of games for attitude change: Searching for factors influencing civil servants' attitudes
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Kleiman, F. (author), Meijer, Sebastiaan (author), Janssen, M.F.W.H.A. (author), Kleiman, F. (author), Meijer, Sebastiaan (author), and Janssen, M.F.W.H.A. (author)
- Abstract
Governments are increasingly using games for civic engagement, decision making, and education. Serious gaming is a type of game that has often been advocated as a means for changing the attitude of its players and can be used for changing the attitude of civil servants. However, the relationship between games and attitude change in civil servants remains unexplored. This paper aims at identifying factors leading to attitude change of civil servants. As hardly any paper is focused on civil servants' attitude change through games, the authors broaden their research to attitude change through games in general. Out of 483 documents, 19 reference papers were analyzed in detail. Eighty-one games were found, and more than 13 different theories were identified containing 30 different influencing factors, which were found mostly to be unrelated and context-dependent. The conceptual dispersion between studies indicates that the resulting overview of factors is a first step towards creating a uniform theory. The results can help governments to design better games., Green Open Access added to TU Delft Institutional Repository ‘You share, we take care!’ – Taverne project https://www.openaccess.nl/en/you-share-we-take-care Otherwise as indicated in the copyright section: the publisher is the copyright holder of this work and the author uses the Dutch legislation to make this work public., Information and Communication Technology
- Published
- 2020
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21. Behavioral factors influencing the opening of government data by civil servants: Initial findings from the literature
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Kleiman, F. (author), Meijer, Sebastiaan (author), Janssen, M.F.W.H.A. (author), Kleiman, F. (author), Meijer, Sebastiaan (author), and Janssen, M.F.W.H.A. (author)
- Abstract
The actual opening of government data is done by civil servants operating within their institutional environment. As such, the institutional environment and their behavior towards the opening of data is key to increase the quality and quantity of open data. However, few studies have analyzed the behavior of government professionals towards open data policy. The objective of this paper is to review the existing literature to find the factors that influences civil servants behaviors towards open data. To identify the drivers and barriers, a literature review was performed listing the most cited papers with "open government data"and "barriers"focusing specific at the behavioral related factors. Even with the increasing of research on the topic of open data, still most of the papers focus on user drivers and barriers rather than on provider challenges. Even less studies focus on the civil servants' individual level of factors influencing their support to the release of governmental data. Most barriers found in the literature are related to infrastructural or technical issues. Whereas some individual level behavioral barriers could be found, including culture, lack of individual incentives and misunderstanding the impact of opening data, broader discussions on social norms, lack of education and experience are still missing in the literature. This paper contributes to the need for deeper understanding of the behavioral factors that influences the civil servants to support the opening of data., Information and Communication Technology
- Published
- 2020
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22. Changing civil servants’ behaviour concerning the opening of governmental data.: Evaluating the effect of a game by comparing civil servants’ intentions before and after a game intervention
- Author
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Kleiman, F. (author), Janssen, M.F.W.H.A. (author), Meijer, Sebastiaan (author), Jansen, S.J.T. (author), Kleiman, F. (author), Janssen, M.F.W.H.A. (author), Meijer, Sebastiaan (author), and Jansen, S.J.T. (author)
- Abstract
Open data policies are increasingly being adopted by governments. However, civil servants find it challenging to comply with open data policies. Gaming can help civil servants to practise opening data and can change their behaviour to support the opening of more data. In this article, the effect of playing a game is evaluated in an experiment in which several factors that influence the opening of data are compared before and after the game. The benefits appeared in unexpected ways and areas. Data management, privacy and security knowledge was transferred using the game, the perception of benefits showed significant changes, and behavioural intention was positively affected. Points for practitioners: Civil servants’ behaviour influences how public policies are enacted. The release of open data by governments is related by many as crucial for increasing public transparency and civic participation, and generating new economic opportunities. Games can influence the attitude of civil servants and, consequently, change governments’ decisions. Transferring knowledge and providing insights from new experiences can influence civil servants’ attitudes to open data. Moreover, governments can use games to influence civil servants’ attitudes., Information and Communication Technology, Housing Systems
- Published
- 2020
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23. Serious gaming for developing open government data policies by local governments
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Kleiman, F., Janssen, M., Meijer, Sebastiaan, Kleiman, F., Janssen, M., and Meijer, Sebastiaan
- Abstract
Developing open government by local government is cumbersome. Many local governments have no policies or are struggling to develop policies enabling to create value from open data. Policy-making is challenging due to the wicked nature of many policy problems, unclear objectives, and the involvement of diverse stakeholders. At the same time governments are opening their policy-making processes for participation by citizens and private companies. The goal of this paper is to present the structure of a game to increase the understanding of open-data policies by local governments. Open Data Policies are aimed at making public data available to be accessed and used by civil society. The game participants can experience the implications of various policies. This should help them to developed better policies., QC 20180828
- Published
- 2018
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24. Gaming to improve public policies by engaging local governments in open data policy-making
- Author
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Kleiman, F. (author), Janssen, M.F.W.H.A. (author), Kleiman, F. (author), and Janssen, M.F.W.H.A. (author)
- Abstract
Open government is a trend of public administration that strengthens the relationship between governments and the public. In open government, the opening of data is the mean to make governmental information accessible for citizens, firms, NGO's and other public bodies. However, one of the challenges is still to get national and local governments to open their data for use. There exist already national governmental policies for open data and research has been developed to support policies and to understand its effects. Opening by default requires that politicians and public servants change their behaviour towards the opening of data. The objective of the present research is to present how games can be used to change the behaviour of local government's civil servants towards providing and using open data to improve the public policies with which they work (overcome cultural resistance against opening data). Therefore, the engagement of civil servants in open data usage will be experimented through games with various interactions forms. Evaluation of what works (and what does not work) will be registered to produce new inputs on how to use gaming exercises on public policies in general and for local Open Data Policies specifically., Information and Communication Technology
- Published
- 2018
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25. Comparison of two coprological methods for the veterinary diagnosis of fasciolosis
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Kleiman, F., Pietrokovsky, S., Gil, S., and Wisnivesky-Colli, C.
- Subjects
cattle ,diagnosis ,método coprológico ,coprological method ,Faciola hepatica ,Fasciola hepatica ,bovino ,diagnóstico - Abstract
The sensitivity and utility of a standard faecal sedimentation method (FSM) and a modified stool sieving staining method (FSSM), both currently employed for the diagnosis of Fasciola hepatica infection were compared. Faecal samples were obtained from 51 bovines of an endemic area for fasciolosis in Southwestern Argentina. Each sample was placed in a recipient containing 5% formalin. Eight millilitres of the suspension, equivalent to 2g of faeces, were used for each of the two methods tested. The number of eggs found per sample was recorded. The proportion of positive samples obtained by the FSSM (27/51) was significantly higher than that by the FSM (11/51) (P
- Published
- 2005
26. The BARD1 C-Terminal Domain Structure and Interactions with Polyadenylation Factor CstF-50
- Author
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Edwards, R. A., primary, Lee, M. S., additional, Tsutakawa, S. E., additional, Williams, R. S., additional, Nazeer, I., additional, Kleiman, F. E., additional, Tainer, J. A., additional, and Glover, J. N., additional
- Published
- 2009
- Full Text
- View/download PDF
27. The 3' processing factor CstF functions in the DNA repair response
- Author
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Mirkin, N., primary, Fonseca, D., additional, Mohammed, S., additional, Cevher, M. A., additional, Manley, J. L., additional, and Kleiman, F. E., additional
- Published
- 2008
- Full Text
- View/download PDF
28. Comparison of two coprological methods for the veterinary diagnosis of fasciolosis
- Author
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Kleiman, F., primary, Pietrokovsky, S., additional, Gil, S., additional, and Wisnivesky-Colli, C., additional
- Published
- 2005
- Full Text
- View/download PDF
29. Subcellular distribution of 125I-insulin by rat liver: specificity and regulatory factors
- Author
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Izzo JL, Sr., primary, Roncone, A. M., additional, and Kleiman, F., additional
- Published
- 1983
- Full Text
- View/download PDF
30. The BARD1 C-terminal domain structure and interactions with polyadenylation factor CstF-50.
- Author
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Edwards RA, Lee MS, Tsutakawa SE, Williams RS, Nazeer I, Kleiman FE, Tainer JA, and Glover JN
- Subjects
- Binding Sites, Cleavage Stimulation Factor genetics, Crystallography, X-Ray, DNA Damage, Humans, In Vitro Techniques, Models, Molecular, Polyadenylation, Protein Interaction Domains and Motifs, Protein Structure, Tertiary, RNA Processing, Post-Transcriptional, Recombinant Fusion Proteins chemistry, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins metabolism, Scattering, Small Angle, Tumor Suppressor Proteins genetics, Ubiquitin-Protein Ligases genetics, X-Ray Diffraction, Cleavage Stimulation Factor chemistry, Cleavage Stimulation Factor metabolism, Tumor Suppressor Proteins chemistry, Tumor Suppressor Proteins metabolism, Ubiquitin-Protein Ligases chemistry, Ubiquitin-Protein Ligases metabolism
- Abstract
The BARD1 N-terminal RING domain binds BRCA1 while the BARD1 C-terminal ankyrin and tandem BRCT repeat domains bind CstF-50 to modulate mRNA processing and RNAP II stability in response to DNA damage. Here we characterize the BARD1 structural biochemistry responsible for CstF-50 binding. The crystal structure of the BARD1 BRCT domain uncovers a degenerate phosphopeptide binding pocket lacking the key arginine required for phosphopeptide interactions in other BRCT proteins. Small angle X-ray scattering together with limited proteolysis results indicates that ankyrin and BRCT domains are linked by a flexible tether and do not adopt a fixed orientation relative to one another. Protein pull-down experiments utilizing a series of purified BARD1 deletion mutants indicate that interactions between the CstF-50 WD-40 domain and BARD1 involve the ankyrin-BRCT linker but do not require ankyrin or BRCT domains. The structural plasticity imparted by the ANK-BRCT linker helps to explain the regulated assembly of different protein BARD1 complexes with distinct functions in DNA damage signaling including BARD1-dependent induction of apoptosis plus p53 stabilization and interactions. BARD1 architecture and plasticity imparted by the ANK-BRCT linker are suitable to allow the BARD1 C-terminus to act as a hub with multiple binding sites to integrate diverse DNA damage signals directly to RNA polymerase.
- Published
- 2008
- Full Text
- View/download PDF
31. Dynamics of Fasciola hepatica transmission in the Andean Patagonian valleys, Argentina.
- Author
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Kleiman F, Pietrokovsky S, Prepelitchi L, Carbajo AE, and Wisnivesky-Colli C
- Subjects
- Animal Husbandry, Animals, Anthelmintics therapeutic use, Argentina epidemiology, Cattle, Cattle Diseases epidemiology, Cattle Diseases prevention & control, Fascioliasis epidemiology, Fascioliasis parasitology, Fascioliasis transmission, Female, Population Dynamics, Seasons, Time Factors, Cattle Diseases parasitology, Cattle Diseases transmission, Fasciola hepatica physiology, Fascioliasis veterinary, Snails parasitology
- Abstract
We described the transmission dynamics of Fasciola hepatica at its southern distribution range. Studies of prevalence and egg output in cattle and population dynamics and infection in snails were performed in a farm in the Andean Patagonian valleys, Argentina, between December 1998 and February 2002. Snail surveys were conducted from spring to autumn. Infection was diagnosed coprologically in the whole herd at the beginning and end of the study, and in a cohort of heifers at the beginning and end of 2001. A twice-a-year anthelmintic treatment was implemented in 1999. The relationship of the variables mentioned above with temperature and rainfall was determined. Lymnaea viatrix showed a life-span of about 15 months and an annual pattern of population dynamics. Specimens were frequently found in temporary environments and lagoons, and rarely in streams. Snail abundance and soil-water availability were directly related in temporary environments and inversely related in lagoons. Overall prevalence in L. viatrix was 0.67% (range: 0.9-14%) and infection was detected in summer and autumn. At the beginning of the study, calves were the least infected age group (15%). Prevalences and median egg counts in grazing animals were similar at the beginning (heifers: 81%, 3.3 epg; cows: 60%, 1.3 epg) and end of the study (heifers and cows: around 51%, 1 epg). Likewise, the prevalence in the cohort of heifers remained similar (around 40%) between surveys. Transmission to cattle was highly effective despite of the short activity period and the low infection rate of snails, and the regular anthelminthic treatment. There would be two seasonal transmission peaks, one in summer-autumn, when infected snails were present, and the other in early spring due to overwintering metacercariae. Some recommendations based on the climatic conditions of the region are provided to minimize snail infection and ultimately to reduce the incidence of fasciolosis in cattle.
- Published
- 2007
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32. [A focus study from a case of human fascioliasis in Neuquén].
- Author
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Rubel D, Prepelitchi L, Kleiman F, Carnevale S, and Wisnivesky-Colli C
- Subjects
- Adult, Animals, Cattle, Enzyme-Linked Immunosorbent Assay, Fascioliasis blood, Fascioliasis veterinary, Feces parasitology, Female, Food Parasitology, Goats, Humans, Lymnaea parasitology, Nasturtium parasitology, Parasite Egg Count, Sheep, Statistics, Nonparametric, Cattle Diseases parasitology, Fasciola hepatica isolation & purification, Fascioliasis etiology
- Abstract
An epidemiological focal study was performed in Loncopué, Neuquén, Argentina, in November 2002 to detect the origin of the infection in a human case of fascioliasis confirmed by an indirect-ELISA test, six months before the study. Thirty five individual fecal samples were taken from domestic livestock, and watercress plants and snails were collected from the irrigation ditches connected to a main canal in the surroundings of the patient's house. A new blood sample was taken from the already recovered patient. The patient was still seropositive to Fasciola hepatica antigens. No metacercariae were found in the 222 watercress leaves checked. All the snails collected (n=130) were identified as Lymnaea viatrix and two out of 101 (2%) were infected with F. hepatica larvae. Coprological analysis showed F. hepatica eggs in 100% of goats (10/10), 82% of sheep (9/11) and 86% of bovines (6/7). The number of eggs per gram shed by positive goats (median=20.7, Q1=6.2, Q3=34.5) and sheep (4, 1.8, 13) was significantly higher than in cows (0.3, 0.3, 1.7) (p < 0.01). Local veterinary control programs were apparently not effective in this case. Anthelmintics used and treatment schedule should be revised and small herds raised at households should also be included and treated.
- Published
- 2005
33. Southernmost finding of Lymnaea viatrix orbigny, 1835 (Pulmonata: Lymnaeidae), intermediate host of Fasciola hepatica (Linnaeus, 1758) (Trematoda: Digenea), in urban and rural areas of Patagonia, Argentina.
- Author
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Kleiman F, Pietrokovsky S, Paraense WL, and Wisnivesky-Colli C
- Subjects
- Animals, Argentina, Rats, Rats, Wistar, Fasciola hepatica isolation & purification, Lymnaea parasitology
- Abstract
We report the first finding of Lymnaea viatrix south of parallel 41 masculine S, in rural and urban areas from Argentina. Ninety snails were collected during year 2000, from a concrete pond at a Public Square in El Bolsón Village, Río Negro province, and 811 snails in November 1999, and during 2000 from waterbodies within a farm at Cholila locality, Chubut province. Fasciola hepatica infection was detected in 0.9% snails from the rural area. We discuss the potential risk of L. viatrix to public health in urban areas and its epidemiological importance in rural areas of the Andean Patagonian region.
- Published
- 2004
- Full Text
- View/download PDF
34. First report of Lymnaea columella Say, 1817 (Pulmonata: Lymnaeidae) naturally infected with Fasciola hepatica (Linnaeus,1758) (Trematoda: Digenea) in Argentina.
- Author
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Prepelitchi L, Kleiman F, Pietrokovsky SM, Moriena RA, Racioppi O, Alvarez J, and Wisnivesky-Colli C
- Subjects
- Animals, Argentina, Rats, Rats, Wistar, Fasciola hepatica isolation & purification, Lymnaea parasitology
- Abstract
We report the first evidence of natural infection of Lymnaea columella with Fasciola hepatica in Argentina. A sample of 601 snails was collected in May 2003 in northeastern Corrientes, a province bounded on the north by Paraguay, on the east by Brazil and on the southeast by Uruguay. Among 500 examined snails, 44 (8.8%) were exclusively infected with F. hepatica. Parasite identification was based on morphological features of cercariae from snails, and of eggs and adult flukes from Wistar rats. We discuss the events suggesting that an enzootic transmission cycle of F. hepatica has been recently established in northeastern Corrientes.
- Published
- 2003
- Full Text
- View/download PDF
35. The BARD1-CstF-50 interaction links mRNA 3' end formation to DNA damage and tumor suppression.
- Author
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Kleiman FE and Manley JL
- Subjects
- Germ-Line Mutation physiology, HeLa Cells, Humans, In Vitro Techniques, RNA, Messenger genetics, mRNA Cleavage and Polyadenylation Factors, Carrier Proteins genetics, Carrier Proteins metabolism, DNA Damage physiology, Genes, Tumor Suppressor genetics, RNA-Binding Proteins genetics, RNA-Binding Proteins metabolism, Tumor Suppressor Proteins, Ubiquitin-Protein Ligases
- Abstract
The mRNA polyadenylation factor CstF interacts with the BRCA1-associated protein BARD1, and this interaction represses the nuclear mRNA polyadenylation machinery in vitro. Given the suspected role of BRCA1/BARD1 in DNA repair, we tested whether inhibition of mRNA processing is linked to DNA damage. Strikingly, we found that 3' cleavage in extracts from cells treated with hydroxyurea or ultraviolet light was strongly, but transiently, inhibited. Although no changes were detected in CstF, BARD1, and BRCA1 protein levels, increased amounts of a CstF/BARD1/BRCA1 complex were detected. Supporting the physiological significance of these results, a previously identified tumor-associated germline mutation in BARD1 (Gln564His) reduced binding to CstF and abrogated inhibition of polyadenylation. Together these results indicate a link between mRNA 3' processing and DNA repair and tumor suppression.
- Published
- 2001
- Full Text
- View/download PDF
36. Functional interaction of BRCA1-associated BARD1 with polyadenylation factor CstF-50.
- Author
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Kleiman FE and Manley JL
- Subjects
- Antibodies, Monoclonal, Carrier Proteins chemistry, Carrier Proteins genetics, Cell Nucleus metabolism, DNA Damage, DNA Repair, DNA-Binding Proteins metabolism, HeLa Cells, Humans, Proliferating Cell Nuclear Antigen metabolism, RNA-Binding Proteins chemistry, RNA-Binding Proteins genetics, Rad51 Recombinase, Recombinant Fusion Proteins metabolism, Repetitive Sequences, Amino Acid, Zinc Fingers, mRNA Cleavage and Polyadenylation Factors, Carrier Proteins metabolism, Poly A metabolism, RNA Polymerase II metabolism, RNA Precursors metabolism, RNA, Messenger metabolism, RNA-Binding Proteins metabolism, Tumor Suppressor Proteins, Ubiquitin-Protein Ligases
- Abstract
Polyadenylation of messenger RNA precursors requires a complex protein machinery that is closely integrated with the even more complex transcriptional apparatus. Here a polyadenylation factor, CstF-50 (cleavage stimulation factor), is shown to interact in vitro and in intact cells with a nuclear protein of previously unknown function, BRCA1-associated RING domain protein (BARD1). The BARD1-CstF-50 interaction inhibits polyadenylation in vitro. BARD1, like CstF-50, also interacts with RNA polymerase II. These results indicate that BARD1-mediated inhibition of polyadenylation may prevent inappropriate RNA processing during transcription, perhaps at sites of DNA repair, and they reveal an unanticipated integration of diverse nuclear events.
- Published
- 1999
- Full Text
- View/download PDF
37. A frequent TG deletion near the polyadenylation signal of the human HEXB gene: occurrence of an irregular DNA structure and conserved nucleotide sequence motif in the 3' untranslated region.
- Author
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Kleiman FE, Ramírez AO, Dodelson de Kremer R, Gravel RA, and Argaraña CE
- Subjects
- 3' Untranslated Regions, Animals, Argentina, DNA chemistry, Female, Gene Frequency, Genetic Carrier Screening, Guanine, Hexosaminidase B, Humans, Male, Mammals, Nucleic Acid Conformation, Poly A metabolism, Reverse Transcriptase Polymerase Chain Reaction, Thymine, DNA metabolism, Sequence Deletion, beta-N-Acetylhexosaminidases genetics
- Abstract
While screening for new mutations in the HEXB gene, which encodes the beta-subunit of beta-hexosaminidase, a TG deletion (deltaTG) was found in the 3' untranslated region (3'UTR) of the gene, 7 bp upstream from the polyadenylation signal. Examination of DNA samples of 145 unrelated Argentinean individuals from different racial backgrounds showed that the deltaTG allele was present with a frequency of approximately 0.1, compared with the wild-type (WT) allele. The deletion was not associated with infantile or variant forms of Sandhoff disease when present in combination with a deleterious allele. Total Hex and Hex B enzymatic activities measured in individuals heterozygous for deltaTG and a null allele, IVS-2 + 1G-->A (G-->A), were approximately 30% lower than the activities of G-->A/WT individuals. Analysis of the HEXB mRNA from leukocytes of deltaTG/WT individuals by RT-PCR of the 3'UTR showed that the deltaTG allele is present at lower level than the WT allele. By polyacrylamide gel electrophoresis, it was determined that a PCR fragment containing the +TG version of the 3'UTR of the HEXB gene had an irregular structure. On inspection of genes containing a TG dinucleotide upstream from the polyadenylation signal we found that this dinucleotide was part of a conserved sequence (TGTTTT) immersed in a A/T-rich region. This sequence arrangement was present in more than 40% analyzed eukaryotic mRNAs, including in the human, mouse and cat HEXB genes. The significance of the TG deletion in reference to Sandhoff disease as well as the possible functional role of the consensus sequence and the DNA structure of the 3'UTR are considered.
- Published
- 1998
- Full Text
- View/download PDF
38. Sandhoff disease in Argentina: high frequency of a splice site mutation in the HEXB gene and correlation between enzyme and DNA-based tests for heterozygote detection.
- Author
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Kleiman FE, de Kremer RD, de Ramirez AO, Gravel RA, and Argaraña CE
- Subjects
- Argentina epidemiology, Clinical Enzyme Tests, DNA Mutational Analysis, Gene Frequency, Hexosaminidase B, Humans, Incidence, Leukocytes enzymology, Polymerase Chain Reaction, RNA Splicing genetics, Sandhoff Disease diagnosis, Sandhoff Disease epidemiology, beta-N-Acetylhexosaminidases genetics, Genetic Carrier Screening, Mutation, Sandhoff Disease genetics, beta-N-Acetylhexosaminidases blood
- Abstract
The level of beta-hexosaminidase activity in plasma and leukocytes and the frequency of three known HEXB mutations were studied in an Argentinean deme with high incidence of infantile Sandhoff disease. Two mutations were previously identified in one of two Sandhoff patients from the region, a splice mutation, IVS-2 + 1 G-->A, and a 4-bp deletion, delta CTTT782-785. These mutations, and a 16-kb deletion from the 5' end of the HEXB gene common in non-Argentineans, were screened in 9 Sandhoff patients (all unrelated), 24 obligate heterozygotes, 33 additional individuals belonging to families with affected members, and 64 randomly ascertained individuals from the high risk region. Of 31 independent alleles examined, including those of the two patients previously reported, 30 had the IVS-2 splice mutation and only the originally reported patient had the delta CTTT deletion. The 16-kb deletion was not observed. Further, among the 57 unaffected members of families with a previous history of Sandhoff disease, and absolute correlation was found between carrier diagnosis by enzyme assay of leukocytes and the DNA-based tests for mutation. One of the 64 controls was classified as a carrier by enzyme assay but did not have one of the three mutations screened. We conclude that a single mutation predominates in this Argentinean population and that the DNA-based test can be an effective supplement or alternative to enzyme-based testing.
- Published
- 1994
- Full Text
- View/download PDF
39. Toothpaste discoloration of composite resin in vitro.
- Author
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Speiser AM, Kleiman MA, Saczawa AF, and Kleiman F
- Subjects
- Color, Composite Resins, Dentifrices adverse effects, Toothpastes adverse effects
- Published
- 1983
40. [Levomepromazine in anesthesiological practice].
- Author
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KLEIMAN F
- Subjects
- Anesthesia, Anesthesiology, Methotrimeprazine, Phenothiazines therapy
- Published
- 1961
41. Obstetric anesthesia; trichlorethylene.
- Author
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KLEIMAN F
- Subjects
- Humans, Anesthesia, Anesthesiology, Trichloroethylene
- Published
- 1949
42. [Effects of anesthesia on the newborn infant].
- Author
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KLEIMAN F
- Subjects
- Female, Infant, Newborn physiology, Pregnancy, Anesthesia, Anesthesia, Obstetrical complications, Anesthesiology, Pregnancy Complications
- Published
- 1961
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