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Fragestellung: Die Goniometermessung gilt in der klinischen Praxis als Standardverfahren zur Messung des Bewegungsumfangs (ROM) des oberen Sprunggelenks und hat damit unmittelbar Auswirkungen auf Diagnose und Therapieentscheidungen. Betrachtet man die Inter- und Intra-Rater-Reliabilität der Goniometermessung [zum vollständigen Text gelangen Sie über die oben angegebene URL]

Seasonal exposure to birch pollen (BP) is a major cause of pollinosis. The specific role of Toll-like receptor 4 (TLR4) in BP-induced allergic inflammation and the identification of key factors in birch pollen extracts (BPE) initiating this process remain to be explored. This study aimed to examine (i) the importance of TLR4 for dendritic cell (DC) activation by BPE, (ii) the extent of the contribution of BPE-derived lipopolysaccharide (LPS) and other potential TLR4 adjuvant(s) in BPE, and (iii) the relevance of the TLR4-dependent activation of BPE-stimulated DCs in the initiation of an adaptive immune response. In vitro, activation of murine bone marrow-derived DCs (BMDCs) and human monocyte-derived DCs by BPE or the equivalent LPS (nLPS) was analyzed by flow cytometry. Polymyxin B (PMB), a TLR4 antagonist and TLR4-deficient BMDCs were used to investigate the TLR4 signaling in DC activation. The immunostimulatory activity of BPE was compared to protein-/lipid-depleted BPE-fractions. In co-cultures of BPE-pulsed BMDCs and Bet v 1-specific hybridoma T cells, the influence of the TLR4-dependent DC activation on T cell activation was analyzed. In vivo immunization of IL-4 reporter mice was conducted to study BPE-induced Th2 polarization upon PMB pre-treatment. Murine and human DC activation induced by either BPE or nLPS was inhibited by the TLR4 antagonist or by PMB, and abrogated in TLR4-deficient BMDCs compared to wild-type BMDCs. The lipid-free but not the protein-free fraction showed a reduced capacity to activate the TLR4 signaling and murine DCs. In human DCs, nLPS only partially reproduced the BPE-induced activation intensity. BPE-primed BMDCs efficiently stimulated T cell activation, which was repressed by the TLR4 antagonist or PMB, and the addition of nLPS to Bet v 1 did not reproduce the effect of BPE. In vivo, immunization with BPE induced a significant Th2 polarization, whereas administration of BPE pre-incubated with PMB showed a decreased tendency. These findings suggest that TLR4 is a major pathway by which BPE triggers DC activation that is involved in the initiation of adaptive immune responses. Further characterization of these BP-derived TLR4 adjuvants could provide new candidates for therapeutic strategies targeting specific mechanisms in BP-induced allergic inflammation.

SSCI-VIDE+ING+ABU:DFA:YSC; International audience; Beyond the formulation of catalytic active phases, the design of the catalyst carrier or support itself is an interesting opportunity for stability and effectiveness improvements [1]. As the overall transport of the reactants and products is key, the diffusion has to be controlled at the scale of the micro and mesopores to overcome mass transport limitations, i.e. debottlenecking the catalyst activity, but also at the scale of the macropores. Therefore, to be efficient, all pore levels need to be interconnected.

Fragestellung: Die Anzahl von Revisionsoperationen bei Hüftprothesen steigt international an. Die Wahl der Operationstechniken und Implantate zur Behandlung der gelockerten Implantate ist dabei von vielen Faktoren abhängig. Zu diesen gehören der Knochensubstanzverlust und der Weichteilschaden.[zum vollständigen Text gelangen Sie über die oben angegebene URL], Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2018)

Fragestellung: In unzementierten Totalhüftprothesen ist eine niedrige Knochendichte mit aseptischer Lockerung assoziiert. In der Regel wird die Knochendichte mittels dual-energy X-ray absorptiometry (DEXA) oder einem quantitativen CT gemessen. Dies stellt einen präoperativen Mehraufwand und[zum vollständigen Text gelangen Sie über die oben angegebene URL], Deutscher Kongress für Orthopädie und Unfallchirurgie (DKOU 2018)