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267 results on '"Knoblich, Jürgen A"'

3. In vitro modeling of the human dopaminergic system using spatially arranged ventral midbrain–striatum–cortex assembloids

Author

Reumann, Daniel, Krauditsch, Christian, Novatchkova, Maria, Sozzi, Edoardo, Wong, Sakurako Nagumo, Zabolocki, Michael, Priouret, Marthe, Doleschall, Balint, Ritzau-Reid, Kaja I., Piber, Marielle, Morassut, Ilaria, Fieseler, Charles, Fiorenzano, Alessandro, Stevens, Molly M., Zimmer, Manuel, Bardy, Cedric, Parmar, Malin, and Knoblich, Jürgen A.

Published
2023
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4. A nomenclature consensus for nervous system organoids and assembloids.

Author

Pașca, Sergiu, Arlotta, Paola, Camp, J, Cappello, Silvia, Gage, Fred, Knoblich, Jürgen, Park, In-Hyun, Reiner, Orly, Song, Hongjun, Studer, Lorenz, Temple, Sally, Testa, Giuseppe, Treutlein, Barbara, Vaccarino, Flora, Lancaster, Madeline, Ming, Guo-Li, Kriegstein, Arnold, Muotri, Alysson, and Bateup, Helen

Subjects
Consensus, Humans, Models, Biological, Nervous System, Organoids, Pluripotent Stem Cells, Terminology as Topic
Abstract

Self-organizing three-dimensional cellular models derived from human pluripotent stem cells or primary tissue have great potential to provide insights into how the human nervous system develops, what makes it unique and how disorders of the nervous system arise, progress and could be treated. Here, to facilitate progress and improve communication with the scientific community and the public, we clarify and provide a basic framework for the nomenclature of human multicellular models of nervous system development and disease, including organoids, assembloids and transplants.

Published
2022

5. ARID1B controls transcriptional programs of axon projection in an organoid model of the human corpus callosum

Author

Martins-Costa, Catarina, Wiegers, Andrea, Pham, Vincent A., Sidhaye, Jaydeep, Doleschall, Balint, Novatchkova, Maria, Lendl, Thomas, Piber, Marielle, Peer, Angela, Möseneder, Paul, Stuempflen, Marlene, Chow, Siu Yu A., Seidl, Rainer, Prayer, Daniela, Höftberger, Romana, Kasprian, Gregor, Ikeuchi, Yoshiho, Corsini, Nina S., and Knoblich, Jürgen A.

Published
2024
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6. Amplification of human interneuron progenitors promotes brain tumors and neurological defects

Author

Eichmüller, Oliver L, Corsini, Nina S, Vértesy, Ábel, Morassut, Ilaria, Scholl, Theresa, Gruber, Victoria-Elisabeth, Peer, Angela M, Chu, Julia, Novatchkova, Maria, Hainfellner, Johannes A, Paredes, Mercedes F, Feucht, Martha, and Knoblich, Jürgen A

Subjects
Biological Psychology, Biomedical and Clinical Sciences, Psychology, Cancer, Stem Cell Research, Stem Cell Research - Induced Pluripotent Stem Cell, Stem Cell Research - Nonembryonic - Non-Human, Stem Cell Research - Nonembryonic - Human, Brain Cancer, Orphan Drug, Congenital Structural Anomalies, Neurosciences, Pediatric, Tuberous Sclerosis, Brain Disorders, Rare Diseases, 2.1 Biological and endogenous factors, Neurological, Brain, Brain Neoplasms, Carcinogenesis, Cell Lineage, Cell Proliferation, Disease Progression, ErbB Receptors, Gene Expression Profiling, Humans, Induced Pluripotent Stem Cells, Interneurons, Loss of Heterozygosity, Neural Stem Cells, Organoids, RNA-Seq, TOR Serine-Threonine Kinases, Tuberous Sclerosis Complex 1 Protein, Tuberous Sclerosis Complex 2 Protein, General Science & Technology
Abstract

Evolutionary development of the human brain is characterized by the expansion of various brain regions. Here, we show that developmental processes specific to humans are responsible for malformations of cortical development (MCDs), which result in developmental delay and epilepsy in children. We generated a human cerebral organoid model for tuberous sclerosis complex (TSC) and identified a specific neural stem cell type, caudal late interneuron progenitor (CLIP) cells. In TSC, CLIP cells over-proliferate, generating excessive interneurons, brain tumors, and cortical malformations. Epidermal growth factor receptor inhibition reduces tumor burden, identifying potential treatment options for TSC and related disorders. The identification of CLIP cells reveals the extended interneuron generation in the human brain as a vulnerability for disease. In addition, this work demonstrates that analyzing MCDs can reveal fundamental insights into human-specific aspects of brain development.

Published
2022

7. ISSCR Guidelines for Stem Cell Research and Clinical Translation: The 2021 update

Author

Lovell-Badge, Robin, Anthony, Eric, Barker, Roger A, Bubela, Tania, Brivanlou, Ali H, Carpenter, Melissa, Charo, R Alta, Clark, Amander, Clayton, Ellen, Cong, Yali, Daley, George Q, Fu, Jianping, Fujita, Misao, Greenfield, Andy, Goldman, Steve A, Hill, Lori, Hyun, Insoo, Isasi, Rosario, Kahn, Jeffrey, Kato, Kazuto, Kim, Jin-Soo, Kimmelman, Jonathan, Knoblich, Jürgen A, Mathews, Debra, Montserrat, Nuria, Mosher, Jack, Munsie, Megan, Nakauchi, Hiromitsu, Naldini, Luigi, Naughton, Gail, Niakan, Kathy, Ogbogu, Ubaka, Pedersen, Roger, Rivron, Nicolas, Rooke, Heather, Rossant, Janet, Round, Jeff, Saitou, Mitinori, Sipp, Douglas, Steffann, Julie, Sugarman, Jeremy, Surani, Azim, Takahashi, Jun, Tang, Fuchou, Turner, Leigh, Zettler, Patricia J, and Zhai, Xiaomei

Subjects
Biochemistry and Cell Biology, Biological Sciences, Stem Cell Research - Nonembryonic - Non-Human, Stem Cell Research - Nonembryonic - Human, Regenerative Medicine, Stem Cell Research, Generic health relevance, Bioethical Issues, Humans, Policy, Practice Guidelines as Topic, Societies, Scientific, Stem Cells, Clinical Sciences, Biochemistry and cell biology
Abstract

The International Society for Stem Cell Research has updated its Guidelines for Stem Cell Research and Clinical Translation in order to address advances in stem cell science and other relevant fields, together with the associated ethical, social, and policy issues that have arisen since the last update in 2016. While growing to encompass the evolving science, clinical applications of stem cells, and the increasingly complex implications of stem cell research for society, the basic principles underlying the Guidelines remain unchanged, and they will continue to serve as the standard for the field and as a resource for scientists, regulators, funders, physicians, and members of the public, including patients. A summary of the key updates and issues is presented here.

Published
2021

8. Recommendations, guidelines, and best practice for the use of human induced pluripotent stem cells for neuropharmacological studies of neuropsychiatric disorders

Author

Dutan Polit, Lucia, Eidhof, Ilse, McNeill, Rhiannon V., Warre-Cornish, Katherine M., Yde Ohki, Cristine Marie, Walter, Natalie Monet, Sala, Carlo, Verpelli, Chiara, Radtke, Franziska, Galderisi, Silvana, Mucci, Armida, Collo, Ginetta, Edenhofer, Frank, Castrén, Maija L., Réthelyi, János M., Ejlersen, Morten, Hohmann, Sonja Simone, Ilieva, Mirolyuba S., Lukjanska, Renate, Matuleviciute, Rugile, Michel, Tanja Maria, de Vrij, Femke M.S., Kushner, Steven A., Lendemeijer, Bas, Kittel-Schneider, Sarah, Ziegler, Georg C., Gruber-Schoffnegger, Doris, Pasterkamp, R. Jeroen, Kasri, Amal, Potier, Marie-Claude, Knoblich, Jürgen A., Brüstle, Oliver, Peitz, Michael, Pich, Emilio Merlo, Harwood, Adrian J., Abranches, Elsa, Falk, Anna, Vernon, Anthony C., Grünblatt, Edna, and Srivastava, Deepak P.

Published
2023
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15. LifeTime and improving European healthcare through cell-based interceptive medicine

Author

Rajewsky, Nikolaus, Almouzni, Geneviève, Gorski, Stanislaw A., Aerts, Stein, Amit, Ido, Bertero, Michela G., Bock, Christoph, Bredenoord, Annelien L., Cavalli, Giacomo, Chiocca, Susanna, Clevers, Hans, De Strooper, Bart, Eggert, Angelika, Ellenberg, Jan, Fernández, Xosé M., Figlerowicz, Marek, Gasser, Susan M., Hubner, Norbert, Kjems, Jørgen, Knoblich, Jürgen A., Krabbe, Grietje, Lichter, Peter, Linnarsson, Sten, Marine, Jean-Christophe, Marioni, John C., Marti-Renom, Marc A., Netea, Mihai G., Nickel, Dörthe, Nollmann, Marcelo, Novak, Halina R., Parkinson, Helen, Piccolo, Stefano, Pinheiro, Inês, Pombo, Ana, Popp, Christian, Reik, Wolf, Roman-Roman, Sergio, Rosenstiel, Philip, Schultze, Joachim L., Stegle, Oliver, Tanay, Amos, Testa, Giuseppe, Thanos, Dimitris, Theis, Fabian J., Torres-Padilla, Maria-Elena, Valencia, Alfonso, Vallot, Céline, van Oudenaarden, Alexander, Vidal, Marie, and Voet, Thierry

Published
2020
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16. Reflections on the past two decades of neuroscience

Author

Bassett, Danielle S., Cullen, Kathleen E., Eickhoff, Simon B., Farah, Martha J., Goda, Yukiko, Haggard, Patrick, Hu, Hailan, Hurd, Yasmin L., Josselyn, Sheena A., Khakh, Baljit S., Knoblich, Jürgen A., Poirazi, Panayiota, Poldrack, Russell A., Prinz, Marco, Roelfsema, Pieter R., Spires-Jones, Tara L., Sur, Mriganka, and Ueda, Hiroki R.

Published
2020
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21. Neutral competition explains the clonal composition of neural organoids.

Author

Pflug, Florian G., Haendeler, Simon, Esk, Christopher, Lindenhofer, Dominik, Knoblich, Jürgen A., and von Haeseler, Arndt

Subjects
PLURIPOTENT stem cells, ORGANOIDS, HUMAN stem cells, FETAL brain, NEURAL development, SURVIVAL analysis (Biometry)
Abstract

Neural organoids model the development of the human brain and are an indispensable tool for studying neurodevelopment. Whole-organoid lineage tracing has revealed the number of progenies arising from each initial stem cell to be highly diverse, with lineage sizes ranging from one to more than 20,000 cells. This high variability exceeds what can be explained by existing stochastic models of corticogenesis and indicates the existence of an additional source of stochasticity. To explain this variability, we introduce the SAN model which distinguishes Symmetrically diving, Asymmetrically dividing, and Non-proliferating cells. In the SAN model, the additional source of stochasticity is the survival time of a lineage's pool of symmetrically dividing cells. These survival times result from neutral competition within the sub-population of all symmetrically dividing cells. We demonstrate that our model explains the experimentally observed variability of lineage sizes and derive the quantitative relationship between survival time and lineage size. We also show that our model implies the existence of a regulatory mechanism which keeps the size of the symmetrically dividing cell population constant. Our results provide quantitative insight into the clonal composition of neural organoids and how it arises. This is relevant for many applications of neural organoids, and similar processes may occur in other developing tissues both in vitro and in vivo. Author summary: Organoids are tissue that is grown in vitro, but which replicates many aspects of a specific organ. Neural organoids grown from around 20,000 human pluripotent stem cells in particular replicate many aspects of the developing human brain, and have become an important tool for studying human neurodevelopment. Lineage-tracing allows the contribution of each ancestral stem cells to the organoid to be measured quantitatively. Surprisingly, these contributions vary between a single and more than 20,000 cells, which affects many applications of neural organoids. In this paper we study how these variations arise. We introduce the so-called SAN model, named after the symmetrically, asymmetrically, and non-dividing cells it distinguishes. This model explains the large variation in lineage sizes as being caused by neutral competition between symmetrically dividing cells, i.e. by random chance as cells divide and differentiate. Our model helps to understand the growth of neural organoids quantitatively, and provides way to account for the large difference in lineage sizes in applications of neural organoids. Neutral competition as the cause of large lineage size differences may also be relevant beyond neural organoids, and possibly in the development of tissue in vivo. [ABSTRACT FROM AUTHOR]

Published
2024
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30. Mentorship in Science: Response to AlShebli et al., Nature Communications 2020

Author

Barker, Roger, Bronner, Marianne, Doetsch, Fiona, Greco, Valentina, Hochedlinger, Konrad, Knoblich, Juergen, Kriegstein, Arnold, Lebkowski, Jane, Lendahl, Urban, Melton, Doug, Murry, Chuck, Pera, Martin, Pereira, Lygia, Schoeler, Hans, Takanori, Takebe, Wysocka, Joanna, Zon, Leonard, Mummery, Christine, Little, Melissa, Lin, Haifan, Clark, Amander, Zaret, Ken, Srivastava, Deepak, Fuchs, Elaine, Watt, Fiona, and Temple, Sally

Published
2021
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34. Safeguarding gene drive experiments in the laboratory: Multiple stringent confinement strategies should be used whenever possible

Author

Akbari, Omar S., Bellen, Hugo J., Bier, Ethan, Bullock, Simon L., Burt, Austin, Church, George M., Cook, Kevin R., Duchek, Peter, Edwards, Owain R., Esvelt, Kevin M., Gantz, Valentino M., Golic, Kent G., Gratz, Scott J., Harrison, Melissa M., Hayes, Keith R., James, Anthony A., Kaufman, Thomas C., Knoblich, Juergen, Malik, Harmit S., Matthews, Kathy A., O'Connor-Giles, Kate M., Parks, Annette L., Perrimon, Norbert, Port, Fillip, Russell, Steven, Ueda, Ryu, and Wildonger, Jill

Published
2015

41. The HUSH complex controls brain architecture and protocadherin fidelity

Author

Hagelkruys, Astrid, primary, Horrer, Marion, additional, Taubenschmid-Stowers, Jasmin, additional, Kavirayani, Anoop, additional, Novatchkova, Maria, additional, Orthofer, Michael, additional, Pai, Tsung-Pin, additional, Cikes, Domagoj, additional, Zhuk, Sergei, additional, Balmaña, Meritxell, additional, Esk, Christopher, additional, Koglgruber, Rubina, additional, Moeseneder, Paul, additional, Lazovic, Jelena, additional, Zopf, Lydia M., additional, Cronin, Shane J.F., additional, Elling, Ulrich, additional, Knoblich, Jürgen A., additional, and Penninger, Josef M., additional

Published
2022
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