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121 results on '"Ko, Hee-Chul"'

11. Phytochemical content and antioxidant activity in eight citrus cultivars grown in Jeju Island according to harvest time.

Author

Kim, Jae-Won, Ko, Hee Chul, Jang, Mi-Gyeong, Han, Sang Heon, Kim, Hyun Jung, and Kim, Se-Jae

Subjects
*HARVESTING time, *CULTIVARS, *CITRUS, *FLAVONOIDS, *ANTIOXIDANTS, *FRUIT skins, *ISLANDS, *ORANGES
Abstract

This study aimed to compare phytochemical content and antioxidant activities of eight diverse citrus cultivars grown in Jeju Island depending on their harvest time owing to their health benefits. Plant peels, flesh, and leaves were extracted, and their flavonoid contents were measured by HPLC. Flavonoid levels were high in peel followed by leaves and juices. It was highest in immature fruits and rapidly declined during ripening. While total phenolic content in all citrus peels and fleshes reduced during ripening, those of leaves did not significantly decrease. Antioxidant activity matched the total phenolic content. This indicates that immature citrus tissues exhibited the highest flavonoid and phenolic content with high antioxidant activity and can be potentially employed as a readily accessible natural antioxidant source. [ABSTRACT FROM AUTHOR]

Published
2023
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26. Callophyllis japonicaextract improves high-fat diet-induced obesity and inhibits adipogenesis in 3T3-L1 cells

Author

Ko， Hee Chul, Sungil Kang, and Se-Jae Kim

Subjects
medicine.medical_specialty, Triglyceride, 3T3-L1 Cells, Ethyl acetate, Adipose tissue, medicine.disease, Obesity, General Biochemistry, Genetics and Molecular Biology, chemistry.chemical_compound, Endocrinology, chemistry, Callophyllis japonica, Adipogenesis, Lactate dehydrogenase, Internal medicine, medicine, Animal Science and Zoology
Abstract

The anti-obesity potential of an ethanolic extract of the edible red alga Callophyllis japonica extract (CJE) was investigated in mice fed a high-fat diet (HFD). CJE administration into HFD mice revealed suppression of body weight, adipose tissue weight, serum total cholesterol, triglyceride, and glucose levels in a dose-dependent manner. Also, it reduced serum levels of glutamic pyruvic transaminase, glutamic oxaloacetic transaminase, and lactate dehydrogenase, as well as the accumulation of fatty droplets in liver tissue. CJE and its ethyl acetate fraction inhibited adipogenesis in 3T3-L1 adipocytes by down-regulating the adipocyte-specific transcriptional regulators. Taken together, these results suggest that CJE reduces obesity in mice fed an HFD by inhibiting lipid accumulation and adipogenesis in the adipose tissues.

Published
2012

48. Callophyllis japonica extract improves high-fat diet-induced obesity and inhibits adipogenesis in 3T3-L1 cells.

Author

Kang, Seong-Il, Shin, Hye-Sun, Kim, Hyo-Min, Yoon, Seon-A, Kang, Seung-Woo, Ko, Hee-Chul, and Kim, Se-Jae

Subjects
RED algae, OBESITY treatment, ADIPOGENESIS, ADIPOSE tissues, FAT cells, LACTATE dehydrogenase
Abstract

The anti-obesity potential of an ethanolic extract of the edible red alga Callophyllis japonica extract (CJE) was investigated in mice fed a high-fat diet (HFD). CJE administration into HFD mice revealed suppression of body weight, adipose tissue weight, serum total cholesterol, triglyceride, and glucose levels in a dose-dependent manner. Also, it reduced serum levels of glutamic pyruvic transaminase, glutamic oxaloacetic transaminase, and lactate dehydrogenase, as well as the accumulation of fatty droplets in liver tissue. CJE and its ethyl acetate fraction inhibited adipogenesis in 3T3-L1 adipocytes by down-regulating the adipocyte-specific transcriptional regulators. Taken together, these results suggest that CJE reduces obesity in mice fed an HFD by inhibiting lipid accumulation and adipogenesis in the adipose tissues. [ABSTRACT FROM AUTHOR]

Published
2012
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49. Preparation of a polymethoxyflavone-rich fraction (PRF) of Citrus sunki Hort. ex Tanaka and its antiproliferative effects

Author

Ko, Hee-Chul, Jang, Mi-Gyeong, Kang, Chang-Hee, Lee, Nam-Ho, Kang, Seong-Il, Lee, Sun-Ryung, Park, Deok-Bae, and Kim, Se-Jae

Subjects
*CHEMICAL peel, *FLAVONOIDS, *CITRUS, *NUCLEAR magnetic resonance, *FUNCTIONAL foods, *FOOD composition
Abstract

Abstract: The peel of Citrus sunki is a rich source of polymethoxyflavones (PMFs). In this study, a PMF-rich fraction (PRF) was obtained from the peels of C. sunki by hot-water extraction and subsequent fractionation with n-hexane. Six PMFs and two demethylated PMFs were isolated by semi-preparative HPLC, and their structures were identified by 1H NMR and 13C NMR. To define the synergistic effects of PRF, the PRF and its eight individual components were assessed for antiproliferative activity using HL-60 cells. The PRF showed greater growth inhibitory activity (IC 50 value=25.14μg/ml), compared with its individual components. Flow cytometric analysis confirmed that the PRF significantly increased the percentage of sub-G1 cells (30.1% at 25μg/ml). Our study provides an efficient method for preparing a PRF for use as a functional food ingredient. [Copyright &y& Elsevier]

Published
2010
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50. Polymethoxyflavone-rich Fraction from Citrus sunkiLeaves Alleviates Renal Dysfunction in Mice with Unilateral Ureteral Obstruction

Author

Baek, Songyee, Jang, Mi Gyeong, Kim, Jae-Won, Ko, Hee Chul, Nam, Mi Hyun, Hur, Sung-Pyo, Park, Soo Ah, and Kim, Se-Jae

Abstract

Polymethoxyflavones (PMFs) are flavonoid compounds present in citrus plants that are proposed to be advantageous to human health. However, the advantageous effects of PMFs in the context of renal dysfunction are unclear. In this study, we made a PMF-rich fraction (PRF) from the leaves of Citrus sunkiHort ex. Tanaka and identified its components using liquid chromatography and mass spectrometry. We then investigated the effect of PRF—comprising 9 types of PMF—on renal dysfunction induced by unilateral ureteral obstruction (UUO) in mice. Animals were divided into four experimental groups (n= 7 per group): I) sham-operated group (Sham); II) UUO group (UUO); III) UUO + Enalapril 0.1 mg/1 mL (UUO + Enap); IV) UUO + PRF 100 mg/kg/day (UUO + PRF). All mice were orally administered with the drugs once a day from 7 days before UUO to 1 week after UUO. After the experiments were over, serum and tissues were taken for biochemical and histological analysis. PRF promoted the recovery of body weight in the background of UUO. Biochemical and histological analysis revealed that PRF ameliorated UUO-induced renal dysfunction and moderately reversed inflammation and tubulointerstitial fibrosis. Further, PRF inhibited the expression of endothelial nitric oxide synthase (eNOS), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), transforming growth factor-β (TGF-β), collagen I (Col-I), and collagen IV (Col-IV). These results suggest that PRF improves UUO-induced renal dysfunction by regulating the expression of inflammatory and fibrotic response-related genes.

Published
2022
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