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Protein-protein interactions and interactions with other molecules including DNA and RNA, play an important role in a range of biological activities and processes in all living cells. Understanding of protein-protein interactions, new approaches, and tools for their modulations are valuable for medicine, biotechnology, and drug development. We used the interleukin-10 family of cytokines as a model system for our research of biological interactions and modulation of their functions. A key prerequisite to study biological processes and a detailed understanding of biomolecular interactions is a recombinant protein that is stable under a broad range of conditions. Recombinant protein expression in sufficient yield and quality is often a challenging task. Therefore, we aimed at developing new approaches in protein design and production. In the first part of our study, we modified IL-24, a member of the IL-10 family to increase its expression and stability. We demonstrated that protein engineering is a powerful tool in research of difficult protein targets. In the second part of our study, we adopted new approaches in designing new protein scaffolds suitable for use in the ribosome and yeast display techniques. Protein scaffolds have become promising alternatives to antibodies in protein drug...

This diploma thesis focuses on the issue of revisions of real estate cadastre data. The beginning of the work is devoted to a brief summary of land records, from historical to contemporary. Furthermore, the work addresses the revision of real estate cadastre data in the area of Ratibořské Hory. The attention of the diploma thesis is paid mainly to the finding of inconsistencies in the geometric determination of bui-ldings. At the end of the work is a statistical evaluation of the data.

Cílem této teoreticko-metodologické studie je vymezit didaktický konstrukt dynamická místa kurikula, zasadit ho do širšího teoretického rámce, charakterizovat s ním spojené metody výzkumu a uvést příklady dobré praxe. V úvodní části příspěvku definujeme pojem dynamická místa a zasazujeme jej do kontextu etablovaných konstruktů jako je obsahová transformace nebo modernizace kurikula. V této části studie také diskutujeme roli formálního a neformálního vzdělávání při řešení dynamických míst kurikula. Další část příspěvku je zaměřena metodologicky a věnuje se různým způsobům vymezování dynamických míst oborů a jejich výběru a legitimizace pro následnou transformaci. Poslední část se věnuje příkladům dobré praxe. Představujeme zde dva návrhy řešení zjištěných dynamických míst z kurikula fyziky a zeměpisu pro ZŠ. Konstrukt dynamických míst je platný také pro kurikulum středních škol. V textu nicméně klademe důraz především na implementaci dynamických míst do kurikula základních škol, které se dotýká všech žáků. Dynamická místa kurikula v tomto textu představujeme jako potenciální spojnici mezi formálním a neformálním vzděláváním. Prvním důvodem je, že při procesu vymezování a výběru dynamických míst se nabízí spolupráce zástupců formálního vzdělávání (oboroví didaktici, učitelé z praxe) i vzdělávání neformálního (pracovníci science center a dalších institucí neformálního vzdělávání). Druhým důvodem je vysoký potenciál institucí neformálního vzdělávání pro ztvárnění dynamických míst díky dobře vybaveným expozicím a laboratořím. Skrze výukové programy nabízené institucemi neformálního vzdělávání školám tak může docházet k aktualizaci kurikula formálního vzdělávání. The aim of this theoretical-methodological study is to defi ne the construct of dynamic areas of curricula, to contextualize it, to describe the research methods of the construct, and to present examples of good practice. Th e term ‘dynamic area’ is defi ned and put into the context of established constructs such as content transformation or the modernization of curricula in the fi rst part of the paper. Th e role of formal and non-formal education in the process of transformation is discussed in the same part of the paper. Th e second part deals with research methods for the specifi cation of dynamic areas of disciplines and methods dealing with the selection and legitimization of the dynamic areas for content transformation. Th e last part focuses on the presentation of examples of good practice. Two suggestions as to how to present selected dynamic areas of geography and physics Kuberská, M., Masopust, P., Kolářová, L., Desenský, P., Slavík, J., Mentlík, P. 313 curricula are described here. Th e dynamic areas of the curricula are presented as a potential connection between formal and non-formal education in this study. Th e fi rst reason for this is because of the advantages of cooperation between providers of formal (lecturers and teachers) and non-formal (for example, science centre staff ) education in the process of the specifi cation and selection of the dynamic areas of the disciplines. Th e second reason is the signifi cant potential of non-formal education institutions for the presentation of selected dynamic areas because of their well-equipped expositions and laboratories. It should be possible to innovate the curricula of formal education through educational programmes off ered by non-formal education institutions to schools.

This thesis deals with the historical changes in the selected area. It is a cadastral area called Vilice. This thesis consists of a research of literature and a practical part. The literary research describes and explains these concepts: landscape and historical development, land use development in the Czech Republic and historical milestones of land use change in the Czech Republic. The practical part deals with a field survey, mapping, a reconstruction and a creation of digital maps in the selected area. It also contains a description of each land use change and their aggregate assessment.

This bachelor thesis deals with the issue of multiple sclerosis. It is a demyelinating disease affecting the central nervous system by inflammatory bearings. The onset of this disease is usually between the years 20 to 40 of age. As a consequence of it patients become disabled. The theoretical part of this thesis is focused on the current status of this problematics. In individual chapters of this bachelor thesis it has been dealts with anatomy and physiology of the nervous system, characteristics, diagnostics, and therapy of multiple sclerosis, and nursing care for patients suffeof it. A qualitative research was used in the practical part. The method of interviewing and technique of semi-structured interview were used to collect the data. The research group consisted of nurses from the Neurology Department and respondents having MS. The first objective of bachelor thesis was to find out the specifics of nursing care for patients having MS. The second objective was to map patients' needs depending on the degree of disability of MS. All the research questions were answered by the executed research survey. This bachelor thesis can serve as a feedback for nurses caring for patients having MS. It can be used as well as a study material for students of nursing, or as an informational material for patients having MS and their families.

While analysing financial health of a company, it is important to consider its specific accounting policy, which affects the interpretation of financial credibility and reliability. This theses analyses the impact of the accounting policy of AERO Vodochody a.s. on financial analysis of this company from 2010 to 2014. Firstly, the thesis analyses the effect of the accounting policy of AERO Vodochody a.s. on its statement of financial position and secondly, it examines the influence of the entries in the statement of financial position on financial indicators. AERO Vodochody a.s. carried out a purchase merger of Technometra Radotín plc., which had very little impact not only on the statement of financial position of the acquiring company, but also on the financial indicators. Significant effect on the statement of financial position of AERO Vodochody a.s. had the spin-off of 43 % of its assent and liabilities. This transaction severly affected the development of depreciation, impairment and reserves, the structure of the statement of financial position and the financial indicators of AERO Vodochody a.s.

The main part of the thesis discusses the occurrence and spreading of non - indigenous species of bentic freshwater invertebrates in the Labe River and it is based on long - term physical, chemical and biological data provided by Povodí Labe, state enterprise. Our results show a decrease in the concentration of nutrients at monitored stretch of the Labe River during the time period between years 2000 and 2011. The relative richness of non - indigenous species in benthic communities was analysed by TCI (Taxonomical Contamination Index), while their relative abundance was analysed by ACI (Abundance Contamination Index). Values of those two indexes increased during the time period between years 1998 and 2012. A detailed analysis is focused on four species - Dikerogammarus villosus, Corbicula fluminea, Jaera istri and Corophium curvispinum. The highest ACI value, as well as the total abundance of D. villosus, C, fluminea and J. istri, has been found at the Velké Březno site. Different values of TCI and ACI indexes and different abundances of specific invasive species can be caused by diverse environmental conditions characterising the studied sites. Significant differences between physical - chemical parameters measured at specific sites has been shown by the Kruskal - Wallis test (α = 0, 05). Only the...

This diploma thesis deals with the problems of communication strategy in a non-profit organisation. The firts part of the thesis discusses background knowledge concerning non-profit organisations, communication, marketing and Public relations and produces the collection of tools and processes for practical use. The second part of the thesis conducts an analysis of the current communication strategy in the inquired organisation. Data analysis also suggests new ways of communication strategy, suitable communication tools and possible ways of publicity and the system of its use. The inquired organisation is Junák -- český skaut, přístav Třináctka Opařany, z. s.. It is the basic organisation unit of Junák and it focuses on the work with children and youth in their free time.

The thesis deals with the role of the state and society in their attitudes to poverty and social exclusion. The introductory part aims at characterising the phenomenon of poverty and social exclusion, and deals with the issue of how the society perceives poverty. It focuses on the concept of the culture of poverty, and attention is given to the need of solidarity, social justice and the social state. The following part of my thesis deals with the extent of poverty and social exclusion in the Czech Republic, as well as possibilities of a social safety net in the Czech Republic. It describes particular examples of the manifestation of social exclusion and poverty in the Czech society, especially in the sphere of housing, opportunities in the labour market and falling into destructive debts. It sheds light on the situation of Romanies as the worst affected population group. The current situation in the Czech Republic shows that the attitude of the society to poverty as a personal fault and failure, impedes mutual solidarity, and generates strategies connected with repression that fail to understand the causes of poverty. For this reason, they cannot be adequately efficient. The society should react to, consider and efficiently solve poverty-related problems. Inspiration may be found in Christian ethics and its concept of human dignity.

The aim of this bachelor thesis was to introduce the musicalness of the pre-school-aged children and its development. Topics connected with fipple flute - its history, application during teaching of the pre-school-aged children and mainly chapters focusing on the correct breathing while playing the instrument, can also be found in the work. The thesis mentions a distinguished Czech teacher of the fipple flute Vaclav Zilka and his cooperator docent Vit Petrů. Professor Zilka has continued his project called "The Wooden Flute" with another project - "Healing Flute" and significantly contributed to the recovering process of the children afflicted with asthma and other breathing difficulties. He has also created a well-organized and a simple-to-use course book fot small flute players, which involves basis of playing the musical instrument as well as several practical breathing exercises.

An introduction of invasive species is considered to be one of the most actual problems of these days and is also related with water environment and water invertebrates. Occurences of many non-native species has been recorded in waters of the Czech Republic and these species can be divided into two groups. Species of the first group are invasive alien species - their ecological patterns (e.g. high growing rates, high reproductive capacity or ability to transfer exotic diseases) make their spreading more successful. North-American crayfish species (Orconectes limosus and Pacifastacus leniusculus), Dreissena polymorpha or Dikerogammarus villosus are the typical representatives of this group. The spreading of these species is very remarkable, since their introduction causes many harmful effects and has negative impacts on native fauna. However, the appearance of other group of water invertebrates has been found in our waters. These are also non-indigenous species, but their spreading is very inconspicuous - they do not reach high population densities and their invasion does not cause negative impacts on native fauna and ecosystems. The evidence of these harmless invasive species has been recorded thanks to the monitoring programs and we possibly wouldn't know about them without this monitoring. The...

This thesis characterizes the products mandatory liability insurance of car traffic, car insurance and some additional insurances of cars. The main objective is the analysis of calculation and of criterias, that determinate the price of insurance. The end point is approach comparison to setting up the price by insurance companies on the Czech insurance market.

The aim of this work is to describe the importance of NAFTA for the foreign trade of Canada and its impact on the territorial and commodity composition of Canada's foreign trade. Trade relations with the USA and Mexico. Factors influencing foreign trade (strong Canadian dollar, financial crisis in the USA, intra-company trade).

Havarijní pojištění je dobrovolné majetkové pojištění, kterým je možné podle klientem zvolené pojistné ochrany krýt riziko havárie, živelní události, odcizení a vandalismu. Na celkovém předepsaném pojistném se podílí havarijní pojištění 13-ti % a meziroční nárůst je v současnosti představován 6-ti %. Ve své práci jsem charakterizovala produkty nejvýznamnějších účastníků na trhu, provedla obsahové i cenové srovnání jednotlivých nabídek a přiblížila, jakým způsobem se liší přístup ke kalkulaci u jednotlivých pojišťoven.

Vývoj obchodu České republiky v letech 2001-2005 s jednotlivými zeměmi EU15 a s EU15 jako celkem (tzn. vývoj exportu, importu, bilance a obratu). Vývoj komoditní struktury s jednotlivými zeměmi a s EU15 jako celkem podle klasifikace SITC a významné změny v této struktuře. Cílem je zhodnotit význam obchodu se zeměmi EU15 pro Českou republiku.

The bachelor thesis deals with the issue of critical thinking and its development at upper secondary school which aims to provide the latest findings in this topic. Thesis is divided into two parts. First one summarizes current findings and researches related to critical thinking in education, describes what critical thinking is, why it is important for upper secondary school pupils, which specifics are brought by its development and which psychological aspects are associated with its development. In the second part, there is a proposal of a research project where the goal is to find out the effect of program Reading and Writing for Critical Thinking, as the only united program in this area in the Czech Republic, to development of critical thinking at upper secondary school pupils

Risky sexual behavior is a phenomenon which can have a negative impact on physical, psychic and social aspects of young girls' lives. This paper is focused on some specific issues of the voluntary premature sexual intercourse of girls before and after the age of 15. Listed are mainly psychological aspects of this phenomenon but also legal regulations relating to early sexual activities of adolescents. The paper deals with the main factors that contribute to the desire for early sexual experiences, further with potential consequences of this behavior and with the importance of prevention of this type of risky sexual conduct. In the empirical part of this work, a qualitative research is proposed which focuses on psychological aspects affecting decision-making of girls with different sexual experience as well as on subjective assessment of their conduct. Furthermore, the research proposal deals with the issue of risk behavior. In addition, in subsequent interviews the tendency for drug abuse will be examined. Keywords: girls adolescence risk behavior sexual risk behavior early sexual experience premature sexual intercourse prevention of sexual risk behavior

Bachelor thesis contains theoretical part and a research design. At the beginning the thesis focuses on current situation in the stability of partnerships in the Czech Republic. It also describes skills, knowledge and attitudes that usually influence the stability of partnerships and marriages. The thesis also deals with the question where and how to gain these skills, knowledge and attitudes. It briefly mentions the influence of a family and in detail focuses on elementary school, its functions and possible actions in this area. Especially discusses the forms, methods and conditions for effective education to stable partnerships and marriages. Presented research design is created as a longitudinal study. Research participants are divided into a control group and a treatment group. Pupils in the research group participate in a long- term program for gaining skills, knowledge and attitudes that could support stability of the individuals' partnerships in the future. The control group does not participate in this program. After 10, 20 and 30 years, the research participants are tested. We find out if the relationships of students who have gone through the program are more stable than relationships of the students in control group.