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28 results on '"Komissarova, Natalia"'

1. Webliometric Indicators of IT Expertise of Different Groups of Authors of Digital Educational Resources

Author

Matukhin, Pavel, Galkina, Aleksandra, Isaeva, Irina, Provorotova, Elena, Komissarova, Natalia, Kacprzyk, Janusz, Series Editor, Pal, Nikhil R., Advisory Editor, Bello Perez, Rafael, Advisory Editor, Corchado, Emilio S., Advisory Editor, Hagras, Hani, Advisory Editor, Kóczy, László T., Advisory Editor, Kreinovich, Vladik, Advisory Editor, Lin, Chin-Teng, Advisory Editor, Lu, Jie, Advisory Editor, Melin, Patricia, Advisory Editor, Nedjah, Nadia, Advisory Editor, Nguyen, Ngoc Thanh, Advisory Editor, Wang, Jun, Advisory Editor, Popkova, Elena G., editor, and Sergi, Bruno S., editor

Published
2020
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2. SUBJECTS OF PUBLIC AUTHORITY IN MATTERS OF COMBATING TERRORISM: LEGAL REGULATION, ADMINISTRATIVE AND CRIMINAL ASPECT.

Author

Sinkevych, Olena, Yurovska, Viktoriia, Komissarova, Natalia, Tkachenko, Oleksandr, and Leheza, Yevhen

Subjects
COUNTERTERRORISM, TERRORISM, CABINET officers, TERRORISTS, PUBLIC administration, STATUS (Law), CRIMINALS, LIQUIDATION
Abstract

Copyright of Ius Humani is the property of Ius Humani and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2024
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17. Inhibition of a Transcriptional Pause by RNA Anchoring

Author

Komissarova, Natalia, Velikodvorskaya, Tatiana, Sen, Ranjan, King, Rodney A., Banik-Maiti, Sarbani, and Weisberg, Robert A.

Subjects
Models, Molecular, Bacteriophage HK022, Base Sequence, Transcription, Genetic, Protein Conformation, Escherichia coli Proteins, Molecular Sequence Data, DNA-Directed RNA Polymerases, Oligonucleotides, Antisense, Article, Viral Proteins, Gene Expression Regulation, Animals, Nucleic Acid Conformation, RNA, Transcriptional Elongation Factors, Uridine
Abstract

We describe a mechanism by which nascent RNA can inhibit transcriptional pausing. PutL RNA of bacteriophage HK022 suppresses transcription termination at downstream terminators and pausing within a nearby U-rich sequence. Using in vitro transcription assays and footprinting techniques, we demonstrate that this pausing results from backtracking of RNA polymerase, and that binding of nascent putL RNA to the enzyme limits backtracking by restricting re-entry of the transcript into the RNA exit channel. The restriction is local and relaxes as the transcript elongates. Our results suggest that putL RNA binds to the surface of RNA polymerase close to the RNA exit channel, a region that includes amino acid residues important for antitermination. Although binding is essential for antipausing and antitermination, these two activities of put differ: antipausing is limited to the immediate vicinity of the putL site, but antitermination is not. We propose that RNA anchoring to the elongation complex is a widespread mechanism of pause regulation.

Published
2008

27. Overextended RNA:DNA hybrid as a negative regulator of RNA polymerase II processivity11Edited by R. Ebright

Author

Kireeva, Maria L, Komissarova, Natalia, and Kashlev, Mikhail

Abstract

An eight nucleotide RNA:DNA hybrid at the 3′ end of the transcript is required for the stability of the elongation complex (EC) of RNA polymerase II. A non-template DNA strand is not needed for the stability of the EC, which contains this minimal hybrid. Here, we apply a recently developed method for promoter-independent assembly of functional EC of RNA polymerase II from synthetic RNA and DNA oligonucleotides to study the minimal composition of the nucleic acid array required for stability of the complex with RNA longer than eight nucleotides. We found that upon RNA extension beyond 14–16 nt in the course of transcription, non-template DNA becomes essential for maintaining a stable EC. Our data suggest that the overextended RNA:DNA hybrid formed in the absence the non-template DNA acts as a negative regulator of EC stability. The dissociation of the EC correlates with the backsliding of the polymerase along the overextended hybrid. The dual role of the hybrid provides a mechanism for the control of a correct nucleic acid architecture in the EC and of RNA polymerase II processivity.

Published
2000
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28. Iodine-125 radioprobing of E. coli RNA polymerase transcription elongation complexes.

Author

Karamychev VN, Tatusov A, Komissarova N, Kashlev M, Neumann RD, Zhurkin VB, and Panyutin IG

Subjects
Base Sequence, Binding Sites, Cytidine Triphosphate chemistry, DNA chemistry, Electrophoresis, Polyacrylamide Gel, Models, Biological, Molecular Sequence Data, Nucleic Acid Conformation, Oligonucleotides chemistry, Biochemistry methods, DNA-Directed RNA Polymerases chemistry, Escherichia coli enzymology, Iodine Radioisotopes chemistry, Transcription, Genetic
Published
2003
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