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[Purpose/Significance] Interdisciplinary research has emerged as a key driver of knowledge innovation, its essence being integration of knowledge from different disciplines. A deep and nuanced understanding of the research content of interdisciplinary knowledge integration is essential to foster innovation beyond traditional academic boundaries. Most of the research reviews on interdisciplinary research summarize theories, concepts and indicators from the macro level, while the content of interdisciplinary knowledge integration at the micro level is scattered, and lacks systematicity and logic. Therefore, this study attempts to classify and summarize the research methods, research content and research ideas involved in order to provide reference for scholars and researchers engaged in interdisciplinary knowledge integration. [Method/Process] Based on Web of Science (WOS) and China National Knowledge Infrastructure (CNKI) database as the primary data sources, we reviewed the relevant literature on interdisciplinary knowledge integration. By clarifying the intrinsic meaning of interdisciplinary knowledge integration, we have systematically reviewed and summarized the existing research findings from three aspects. First, research on the degree of knowledge integration mainly involves measurement methods. Second, research on the content of knowledge integration is based on citations and keywords. Third, research on the process of knowledge integration involves its integration path and process, and the characteristics of the integration stage. On this basis, this paper also summarizes the limitations and challenges of existing research, and provides research perspectives for subsequent research on interdisciplinary knowledge integration. [Results/Conclusions] The existing results have extensively explored the definition, measurement method, content, and process of interdisciplinary knowledge integration, but there are problems such as limited applicability of knowledge integration measurement methods, insufficient semantic disclosure, and the lack of systematic research on the degree of knowledge integration. Future research in this area should pay attention to the following three aspects. First, the applicability of methods for measuring interdisciplinary knowledge integration should be improved in order to better adapt to the development of interdisciplinary knowledge integration. Second, there is also a need to strengthen the study of knowledge integration from the perspective of knowledge increment, examining how new knowledge is created and how it contributes to the advancement of the area. In addition, efforts should be made to explore new theories and methods, grasp the development law of interdisciplinary knowledge integration, and expand the application scope and application value of interdisciplinary knowledge integration.

The overactivation of the osteoclasts is a crucial pathological factor in the development of osteoporosis. MZF1, belonging to the scan-zinc finger family, plays a significant role in various processes associated with tumor malignant progression and acts as an essential transcription factor regulating osteoblast expression. However, the exact role of MZF1 in osteoclasts has not been determined. In this study, the purpose of our study was to elucidate the role of MZF1 in osteoclastogenesis. First, we established MZF1-deficient female mice and evaluated the femur bone phenotype by micro–computed tomography and histological staining. Our findings indicate that MZF1−/− mice exhibited a low bone mass osteoporosis phenotype. RANKL could independently induce the differentiation of RAW264.7 cells into osteoclasts, and we found that the expression level of MZF1 protein decreased gradually. Then, the CRISPR/Cas 9 gene-editing technique was used to build a RAW264.7 cell model with MZF1 knockout, and RANKL was used to independently induce MZF1−/− and wild-type cells to differentiate into mature osteoclasts. Tartrate-resistant acid phosphatase staining and F-actin fluorescence results showed that the MZF1−/− group produced more tartrate-resistant acid phosphatase–positive mature osteoclasts and larger actin rings. The expression of osteoclast-associated genes (including tartrate-resistant acid phosphatase, CTSK, c-Fos, and NFATc1) was evaluated by reverse transcription quantitative polymerase chain reaction and Western blot. The expression of key genes of osteoclast differentiation in the MZF1−/− group was significantly increased. Furthermore, we found that cell viability was increased in the early stages of RANKL-induced cell differentiation in the MZF1−/− group cells. We examined some prevalent ferroptosis markers, including malondialdehyde, glutathione, and intracellular Fe, the active form of iron in the cytoplasm during the early stages of osteoclastogenesis. The results suggest that MZF1 may be involved in osteoclast differentiation by regulating RANKL-induced ferroptosis of osteoclasts. Collectively, our findings shed light on the essential involvement of MZF1 in the regulation of osteoclastogenesis in osteoporosis and provide insights into its potential underlying mechanism. [ABSTRACT FROM AUTHOR]

In order to reveal the change laws of microstructure in apple slices during hot air‐explosion puffing drying, the microstructure images and parameters of apple slices under different drying conditions were obtained by using paraffin sectioning, microscopic observation, and image processing techniques with apples as raw material and CO2 as drying medium. Subsequently, the changes in internal cell and pore structure of dried apple slices were analyzed, as well as the relationship between micro‐parameters of apple slices and macro‐measurement indicators was further obtained. Results indicated that the overall cross‐sectional area, cross‐sectional perimeter, equivalent diameter, and porosity of cells increased by explosion puffing drying, while the cell roundness and wall roughness factors changed relatively little. Meanwhile, macro–micro parameters of expanded apples were fitted and analyzed. Then, the obtained mathematical model showed a nonlinear correlation as experimental and predicted results exhibited good agreement, with determination coefficients R2 more significant than 0.8, which could better expect the macro–microparameters to change in the drying process. The results of this research could provide an essential reference for exploring the change laws of tissue microstructure parameters in apples dried by hot air‐explosion puffing drying, with expansion and rehydration ratios under different experimental conditions, as well as optimizing the puffing drying process and designing corresponding drying equipment. Practical Applications: Fresh apples have high moisture content and are not easy to store, so drying is usually used to reduce the moisture content to extend shelf time and increase economic benefits. For the drying process of fruit and vegetable products, scholars mainly focused on the influence of microstructure parameters on moisture migration, drying rate, heat and mass transfer and other factors, but did not give a quantitative description of how microstructure parameters affect macroscopic parameters. In this article, the relationship between microstructural changes and macroscopic indicators was investigated by taking puffed apple slices as an example. Research could provide an essential reference for exploring the change laws of tissue microstructure parameters in apples dried by hot air‐explosion puffing drying, with expansion and rehydration ratios under different experimental conditions, as well as optimizing the puffing drying process and designing corresponding drying equipment. [ABSTRACT FROM AUTHOR]