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302 results on '"Korchev, Yuri E."'

1. Electrochemical Nanopipette Sensor for In Vitro/In Vivo Detection of Cu2+ Ions

Author

Timoshenko, Roman V., primary, Gorelkin, Petr V., additional, Vaneev, Alexander N., additional, Krasnovskaya, Olga O., additional, Akasov, Roman A., additional, Garanina, Anastasiia S., additional, Khochenkov, Dmitry A., additional, Iakimova, Tamara M., additional, Klyachko, Natalia L., additional, Abakumova, Tatiana O., additional, Shashkovskaya, Vera S., additional, Chaprov, Kirill D., additional, Makarov, Alexander A., additional, Mitkevich, Vladimir A., additional, Takahashi, Yasufumi, additional, Edwards, Christopher R. W., additional, Korchev, Yuri E., additional, and Erofeev, Alexander S., additional

Published
2023
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3. Electrochemical Nanopipette Sensor for In Vitro/In Vivo Detection of Cu2+ Ions.

Author

Timoshenko, Roman V., Gorelkin, Petr V., Vaneev, Alexander N., Krasnovskaya, Olga O., Akasov, Roman A., Garanina, Anastasiia S., Khochenkov, Dmitry A., Iakimova, Tamara M., Klyachko, Natalia L., Abakumova, Tatiana O., Shashkovskaya, Vera S., Chaprov, Kirill D., Makarov, Alexander A., Mitkevich, Vladimir A., Takahashi, Yasufumi, Edwards, Christopher R. W., Korchev, Yuri E., and Erofeev, Alexander S.

Published
2024
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6. Electrochemical Nanopipette Sensor for In Vitro/In Vivo Detection of Cu2+Ions

Author

Timoshenko, Roman V., Gorelkin, Petr V., Vaneev, Alexander N., Krasnovskaya, Olga O., Akasov, Roman A., Garanina, Anastasiia S., Khochenkov, Dmitry A., Iakimova, Tamara M., Klyachko, Natalia L., Abakumova, Tatiana O., Shashkovskaya, Vera S., Chaprov, Kirill D., Makarov, Alexander A., Mitkevich, Vladimir A., Takahashi, Yasufumi, Edwards, Christopher R. W., Korchev, Yuri E., and Erofeev, Alexander S.

Abstract

In vitro/in vivo detection of copper ions is a challenging task but one which is important in the development of new approaches to the diagnosis and treatment of cancer and hereditary diseases such as Alzheimer’s, Wilson’s, etc. In this paper, we present a nanopipette sensor capable of measuring Cu2+ions with a linear range from 0.1 to 10 μM in vitro and in vivo. Using the gold-modified nanopipette sensor with a copper chelating ligand, we evaluated the accumulation ability of the liposomal form of an anticancer Cu-containing complex at three levels of biological organization. First, we detected Cu2+ions in a single cell model of human breast adenocarcinoma MCF-7 and in murine melanoma B16 cells. The insertion of the nanoelectrode did not result in leakage of the cell membrane. We then evaluated the distribution of the Cu-complex in MCF-7 tumor spheroids and found that the diffusion-limited accumulation was a function of the depth, typical for 3D culture. Finally, we demonstrated the use of the sensor for Cu2+ion detection in the brain of an APP/PS1 transgenic mouse model of Alzheimer’s disease and tumor-bearing mice in response to injection (2 mg kg–1) of the liposomal form of the anticancer Cu-containing complex. Enhanced stability and selectivity, as well as distinct copper oxidation peaks, confirmed that the developed sensor is a promising tool for testing various types of biological systems. In summary, this research has demonstrated a minimally invasive electrochemical technique with high temporal resolution that can be used for the study of metabolism of copper or copper-based drugs in vitro and in vivo.

Published
2024
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9. Bifunctional Magnetite–Gold Nanoparticles for Magneto-Mechanical Actuation and Cancer Cell Destruction

Author

Garanina, Anastasiia S., primary, Efremova, Maria V., additional, Machulkin, Alexey E., additional, Lyubin, Evgeny V., additional, Vorobyeva, Natalia S., additional, Zhironkina, Oxana A., additional, Strelkova, Olga S., additional, Kireev, Igor I., additional, Alieva, Irina B., additional, Uzbekov, Rustem E., additional, Agafonov, Viatcheslav N., additional, Shchetinin, Igor V., additional, Fedyanin, Andrey A., additional, Erofeev, Alexander S., additional, Gorelkin, Peter V., additional, Korchev, Yuri E., additional, Savchenko, Alexander G., additional, and Abakumov, Maxim A., additional

Published
2022
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11. Scanning ion-conductance microscopy for studying β-amyloid aggregate formation on living cell surface

Author

Kolmogorov, Vasilii S., primary, Erofeev, Alexander S., additional, Barykin, Evgeny P., additional, Timoshenko, Roman V., additional, Lopatukhina, Elena V., additional, Kozin, Sergey A., additional, Salikhov, Sergey V., additional, Klyachko, Natalia L., additional, Mitkevich, Vladimir A., additional, Edwards, Christopher R.W., additional, Korchev, Yuri E., additional, Makarov, Alexander A., additional, and Gorelkin, Petr V., additional

Published
2022
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12. In Vitro/In VivoElectrochemical Detection of Pt(II) Species

Author

Vaneev, Alexander N., primary, Gorelkin, Petr V., additional, Krasnovskaya, Olga O., additional, Akasov, Roman A., additional, Spector, Daniil V., additional, Lopatukhina, Elena V., additional, Timoshenko, Roman V., additional, Garanina, Anastasiia S., additional, Zhang, Yanjun, additional, Salikhov, Sergey V., additional, Edwards, Christopher R. W., additional, Klyachko, Natalia L., additional, Takahashi, Yasufumi, additional, Majouga, Alexander G., additional, Korchev, Yuri E., additional, and Erofeev, Alexander S., additional

Published
2022
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22. Studying the Local Young's Modulus of PC-3 Cells Via Scanning Ion-Conductance Microscopy

Author

Kolmogorov, Vasilii, primary, Vaneev, Alexander, additional, Efremov, Yuri M., additional, Savin, Nikita, additional, Iakovlev, Aleksei, additional, Alova, Anna, additional, Lavrushkina, Svetlana, additional, Erofeev, Alexander, additional, Gorelkin, Petr, additional, Klyachko, Natalia, additional, Kireev, Igor, additional, Majouga, Alexander, additional, Novak, Pavel, additional, and Korchev, Yuri E., additional

Published
2021
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23. [[beta].sub.2]-adrenergic receptor redistribution in heart failure changes cAMP compartmentation

Author

Nikolaev, Viacheslav O., Moshkov, Alexey, Lyon, Alexander R., Miragoli, Michele, Novak, Pavel, Paur, Helen, Lohse, Martin J., Korchev, Yuri E., Harding, Sian E., and Gorelik, Julia

Subjects
Heart failure -- Development and progression, Cyclic adenylic acid -- Health aspects, Beta adrenoceptors -- Health aspects, Science and technology
Abstract

The [[beta].sub.1]-and [[beta].sub.2]-adrenergic receptors ([beta]ARs) on the surface of cardiomyocytes mediate distinct effects on cardiac function and the development of heart failure by regulating production of the second messenger cyclic adenosine monophosphate (cAMP). The spatial localization in cardiomyocytes of these [beta]ARs, which are coupled to heterotrimeric guanine nucleotide--binding proteins (G proteins), and the functional implications of their localization have been unclear. We combined nanoscale live-cell scanning ion conductance and fluorescente resonance energy transfer microscopy techniques and found that, in cardiomyocytes from healthy adult rats and mice, spatially confined [[beta].sub.2]AR-induced cAMP signals are localized exclusively to the deep transverse tubules, whereas functional [[beta].sub.1]ARs are distributed across the entire cell surface. In cardiomyocytes derived from a rat model of chronic heart failure, [[beta].sub.2]ARs were redistributed from the transverse tubules to the cell crest, which led to diffuse receptor-mediated cAMP signaling. Thus, the redistribution of [[beta].sub.2]ARs in heart failure changes compartmentation of cAMP and might contribute to the failing myocardial phenotype. 10.1126/science.1185988

Published
2010
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25. In Vitro/In Vivo Electrochemical Detection of Pt(II) Species.

Author

Vaneev, Alexander N., Gorelkin, Petr V., Krasnovskaya, Olga O., Akasov, Roman A., Spector, Daniil V., Lopatukhina, Elena V., Timoshenko, Roman V., Garanina, Anastasiia S., Yanjun Zhang, Salikhov, Sergey V., Edwards, Christopher R. W., Klyachko, Natalia L., Yasufumi Takahashi, Majouga, Alexander G., Korchev, Yuri E., and Erofeev, Alexander S.

Published
2022
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26. Mapping mechanical properties of living cells at nanoscale using intrinsic nanopipette–sample force interactions

Author

Kolmogorov, Vasilii S., primary, Erofeev, Alexander S., additional, Woodcock, Emily, additional, Efremov, Yuri M., additional, Iakovlev, Aleksei P., additional, Savin, Nikita A., additional, Alova, Anna V., additional, Lavrushkina, Svetlana V., additional, Kireev, Igor I., additional, Prelovskaya, Alexandra O., additional, Sviderskaya, Elena V., additional, Scaini, Denis, additional, Klyachko, Natalia L., additional, Timashev, Peter S., additional, Takahashi, Yasufumi, additional, Salikhov, Sergey V., additional, Parkhomenko, Yuri N., additional, Majouga, Alexander G., additional, Edwards, Christopher R. W., additional, Novak, Pavel, additional, Korchev, Yuri E., additional, and Gorelkin, Petr V., additional

Published
2021
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29. Characterization of a novel light source for simultaneous optical and scanning ion conductance microscopy

Author

Bruckbauer, Andreas, Ying, Liming, Rothery, Alison M., Korchev, Yuri E., and Klenerman, David

Subjects
Biological specimens, Fluorimetry -- Methods, Ionic solutions -- Usage, Images, Optical -- Analysis, Chemistry
Abstract

We have developed a novel light source suitable for imaging of biological samples. The method is based on the use of a micropipet filled with fluo-3. A fluorogenic complex is formed when fluo-3 meets calcium in the bath solution. The complex is excited by focusing a laser beam at the pipet tip to produce a submicrometer light source. This source is continually renewed at the tip, eliminating problems with photobleaching, and can be controlled by varying the applied potential. We first characterized the light source using fluorescence correlation measurements in order to optimize its properties. We then recorded an image of a model sample under buffer with submicrometer resolution using ion conductance distance control to demonstrate the feasibility of this approach.

Published
2002

31. In Vitro and In Vivo Electrochemical Measurement of Reactive Oxygen Species After Treatment with Anticancer Drugs

Author

Vaneev, Alexander N., primary, Gorelkin, Petr V., additional, Garanina, Anastasiia S., additional, Lopatukhina, Helena V., additional, Vodopyanov, Stepan S., additional, Alova, Anna V., additional, Ryabaya, Oxana O., additional, Akasov, Roman A., additional, Zhang, Yanjun, additional, Novak, Pavel, additional, Salikhov, Sergey V., additional, Abakumov, Maxim A., additional, Takahashi, Yasufumi, additional, Edwards, Christopher R. W., additional, Klyachko, Natalia L., additional, Majouga, Alexander G., additional, Korchev, Yuri E., additional, and Erofeev, Alexander S., additional

Published
2020
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32. High‐Resolution Electrochemical Mapping of the Hydrogen Evolution Reaction on Transition‐Metal Dichalcogenide Nanosheets

Author

Takahashi, Yasufumi, primary, Kobayashi, Yu, additional, Wang, Ziqian, additional, Ito, Yoshikazu, additional, Ota, Masato, additional, Ida, Hiroki, additional, Kumatani, Akichika, additional, Miyazawa, Keisuke, additional, Fujita, Takeshi, additional, Shiku, Hitoshi, additional, Korchev, Yuri E., additional, Miyata, Yasumitsu, additional, Fukuma, Takeshi, additional, Chen, Mingwei, additional, and Matsue, Tomokazu, additional

Published
2020
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34. Measuring Ion Fluxes in Sperm

Author

Darszon, Alberto, primary, Wood, Christopher D., additional, Beltrán, Carmen, additional, Sánchez, Daniel, additional, Rodríguez, Esmeralda, additional, Gorelik, Julia, additional, Korchev, Yuri E., additional, and Nishigaki, Takuya, additional

Published
2004
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35. Angular Approach Scanning Ion Conductance Microscopy

Author

Shevchuk, Andrew, Tokar, Sergiy, Gopal, Sahana, Sanchez-Alonso, Jose L., Tarasov, Andrei I., Vélez-Ortega, A. Catalina, Chiappini, Ciro, Rorsman, Patrik, Stevens, Molly M., Gorelik, Julia, Frolenkov, Gregory I., Klenerman, David, Korchev, Yuri E., Wellcome Trust, and Biotechnology and Biological Sciences Research Council (BBSRC)

Subjects
Adult, Male, Patch-Clamp Techniques, 02 Physical Sciences, Biophysics, Equipment Design, Microscopy, Scanning Probe, 06 Biological Sciences, Culture Media, Rats, Sprague-Dawley, Mice, Micromanipulation, Imaging, Three-Dimensional, Cell Biophysics, Microscopy, Electron, Scanning, Animals, Humans, Nanotechnology, Female, 03 Chemical Sciences, Cells, Cultured, HeLa Cells
Abstract

Scanning ion conductance microscopy (SICM) is a super-resolution live imaging technique that uses a glass nanopipette as an imaging probe to produce three-dimensional (3D) images of cell surface. SICM can be used to analyze cell morphology at nanoscale, follow membrane dynamics, precisely position an imaging nanopipette close to a structure of interest, and use it to obtain ion channel recordings or locally apply stimuli or drugs. Practical implementations of these SICM advantages, however, are often complicated due to the limitations of currently available SICM systems that inherited their design from other scanning probe microscopes in which the scan assembly is placed right above the specimen. Such arrangement makes the setting of optimal illumination necessary for phase contrast or the use of high magnification upright optics difficult. Here, we describe the designs that allow mounting SICM scan head on a standard patch-clamp micromanipulator and imaging the sample at an adjustable approach angle. This angle could be as shallow as the approach angle of a patch-clamp pipette between a water immersion objective and the specimen. Using this angular approach SICM, we obtained topographical images of cells grown on nontransparent nanoneedle arrays, of islets of Langerhans, and of hippocampal neurons under upright optical microscope. We also imaged previously inaccessible areas of cells such as the side surfaces of the hair cell stereocilia and the intercalated disks of isolated cardiac myocytes, and performed targeted patch-clamp recordings from the latter. Thus, our new, to our knowledge, angular approach SICM allows imaging of living cells on nontransparent substrates and a seamless integration with most patch-clamp setups on either inverted or upright microscopes, which would facilitate research in cell biophysics and physiology.

Published
2016
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37. Correlative SICM‐FCM reveals changes in morphology and kinetics of endocytic pits induced by disease‐associated mutations in dynamin

Author

Ali, Tayyibah, primary, Bednarska, Joanna, additional, Vassilopoulos, Stéphane, additional, Tran, Martin, additional, Diakonov, Ivan A., additional, Ziyadeh-Isleem, Azza, additional, Guicheney, Pascale, additional, Gorelik, Julia, additional, Korchev, Yuri E., additional, Reilly, Mary M., additional, Bitoun, Marc, additional, and Shevchuk, Andrew, additional

Published
2019
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38. Nanoscale Imaging of Primary Cilia with Scanning Ion Conductance Microscopy

Author

Zhou, Yuanshu, Saito, Masaki, Miyamoto, Takafumi, Novak, Pavel, Shevchuk, Andrew I, Korchev, Yuri E, Fukuma, Takeshi, and Takahashi, Yasufumi

Abstract

Primary cilia are hair-like sensory organelles whose dimensions and location vary with cell type and culture condition. Herein, we employed scanning ion conductance microscopy (SICM) to visualize the topography of primary cilia from different cell types. By combining SICM with fluorescence imaging, we successfully distinguished between surface cilia that project outward from the cell surface and subsurface cilia that are trapped below it. The nanoscale structure of the ciliary pocket, which cannot be easily identified using a confocal fluorescence microscope, was observed in SICM images. Furthermore, we developed a topographic reconstruction method using current-distance profiles to evaluate the relationship between set point and topographic image and found that a low set point is important for detecting the true topography of a primary cilium using hopping mode SICM.

Published
2024
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41. Simultaneous noncontact topography and electrochemical imaging by SECM/SICM featuring ion current feedback regulation

Author

Takahashi, Yasufumi, Shevchuk, Andrew I., Novak, Pavel, Murakami, Yumi, Shiku, Hitoshi, Korchev, Yuri E., and Matsue, Tomakazu

Subjects
Dextrose -- Chemical properties, Glucose -- Chemical properties, Oxidation-reduction reaction -- Analysis, Peroxidase -- Chemical properties, Peroxidase -- Structure, Scanning electron microscopes -- Usage, Voltammetry -- Usage, Chemistry
Abstract

A hybrid system of scanning electrochemical microscopy (SECM) and scanning ion conductance microscopy (SICM) with ion current feedback nanopositioning control for simultaneous imaging of noncontact topography and spatial distribution of electrochemical species is described. The measurements have shown the distribution of activity of the enzyme spots on uneven surfaces with submicrometer resolution.

Published
2010

42. A renewable nanosensor based on a glass nanopipette

Author

Piper, Joe D., Clarke, Richard W., Korchev, Yuri E., Liming Ying, and Klenerman, David

Subjects
Chemiluminescence -- Analysis, Sodium compounds -- Optical properties, Hydrogen-ion concentration -- Research, Tubes -- Usage, Chemistry
Abstract

The ability to detect both ratiometric and intensity-based fluorescent changes for physiological levels of pH and sodium respectively is demonstrated by employing a simply fabricated nanosensor. The approach should prove to be generally applicable to any fluorescence-based reporter dye and thus a wide range of analytes and concentrations.

Published
2006

43. Microdomain-Specific Modulation of L-Type Calcium Channels Leads to Triggered Ventricular Arrhythmia in Heart Failure

Author

Sanchez-Alonso, Jose L., primary, Bhargava, Anamika, additional, O’Hara, Thomas, additional, Glukhov, Alexey V., additional, Schobesberger, Sophie, additional, Bhogal, Navneet, additional, Sikkel, Markus B., additional, Mansfield, Catherine, additional, Korchev, Yuri E., additional, Lyon, Alexander R., additional, Punjabi, Prakash P., additional, Nikolaev, Viacheslav O., additional, Trayanova, Natalia A., additional, and Gorelik, Julia, additional

Published
2016
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49. Nanoscale visualization of redox activity at lithium-ion battery cathodes

Author

Takahashi, Yasufumi, primary, Kumatani, Akichika, additional, Munakata, Hirokazu, additional, Inomata, Hirotaka, additional, Ito, Komachi, additional, Ino, Kosuke, additional, Shiku, Hitoshi, additional, Unwin, Patrick R., additional, Korchev, Yuri E., additional, Kanamura, Kiyoshi, additional, and Matsue, Tomokazu, additional

Published
2014
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50. An addressable antibody nanoarray produced on a nanostructured surface

Author

Bruckbauer, Andreas; Zhou, Dejian, Kang, Dae-Joon, Korchev, Yuri E., Abell, Chris, and Klenerman, David

Subjects
Biomolecules -- Research, Adsorption -- Methods, Nanotechnology -- Research, Chemistry
Abstract

Nanofabricated surfaces provide a region with different chemical functionality for selective adsorption of biomolecules to produce a nanoarray of biomolecules and also provide a topographic feature enabling one to return to the same position on the surface. It is possible to selectively address by nanopipet delivery of functional antibody, as per the demonstration.

Published
2004

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