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223 results on '"Kotiaho T"'

7. Combined transcriptomics, proteomics and metabolomics analysis identifies metabolic pathways associated with the loss of cardiac regeneration

Author

Talman, V., Teppo, J., Poho, P., Movahedi, P., Vaikkinen, A., Pahikkala, T., Kotiaho, T., Kostiainen, R., Varjosalo, M., Ruskoaho, H., Doctoral Programme in Drug Research, Faculty of Pharmacy, Drug Research Program, Division of Pharmaceutical Chemistry and Technology, Institute of Biotechnology, Tapio Kotiaho / Principal Investigator, Department of Chemistry, Risto Kostiainen / Principal Investigator, Molecular Systems Biology, Division of Pharmacology and Pharmacotherapy, and Regenerative pharmacology group

Subjects
317 Pharmacy, education
Published
2018

10. Mechanism of the oxidation of heptafulvenes to tropones studied by online mass spectrometry and density functional theory calculations

Author

Nilsson, S. M. (Sofia M. E.), Henschel, H. (Henning), Scotti, G. (Gianmario), Haapala, M. (Markus), Kiriazis, A. (Alexandros), Boije af Gennäs, G. (Gustav), Kotiaho, T. (Tapio), Yli-Kauhaluoma, J. (Jari), Nilsson, S. M. (Sofia M. E.), Henschel, H. (Henning), Scotti, G. (Gianmario), Haapala, M. (Markus), Kiriazis, A. (Alexandros), Boije af Gennäs, G. (Gustav), Kotiaho, T. (Tapio), and Yli-Kauhaluoma, J. (Jari)

Abstract

We have identified the most likely reaction mechanism for oxidizing heptafulvenes to the corresponding tropones by experimental and theoretical investigations. The experimental studies were done by coupling a three-dimensional printed miniaturized reactor with an integrated electrospray ionization needle to a mass spectrometer. Using the experimentally observed ions as a basis, nine alternative reaction pathways were investigated with density functional theory calculations. The lowest energy reaction pathway starts with the formation of an epoxide that is opened upon the addition of a second equivalent of the oxidizing species meta-chloroperoxybenzoic acid. The adduct formed then undergoes a Criegee-like rearrangement to yield a positively charged hemiketal, which on deprotonation dissociates into acetone and tropone. Overall, the reaction mechanism resembles a Hock-like rearrangement.

Published
2019

14. Variation in collision cross section of acetate dimers

Author

Pedersen, Christian S., Mauriala, T., Adamov, A., Viitanen, A.-K., Mäkela, J.M., Sysoev, A., Lauritsen, Frants Roager, Kotiaho, T., Pedersen, Christian S., Mauriala, T., Adamov, A., Viitanen, A.-K., Mäkela, J.M., Sysoev, A., Lauritsen, Frants Roager, and Kotiaho, T.

Published
2007

40. Liquid chromatography/atmospheric pressure ionization–mass spectrometry in drug metabolism studies

Author

Kostiainen, R., Kotiaho, T., Kuuranne, T., and Auriola, S.

Abstract

The study of the metabolic fate of drugs is an essential and important part of the drug development process. The analysis of metabolites is a challenging task and several different analytical methods have been used in these studies. However, after the introduction of the atmospheric pressure ionization (API) technique, electrospray and atmospheric pressure chemical ionization, liquid chromatography/mass spectrometry (LC/MS) has become an important and widely used method in the analysis of metabolites owing to its superior specificity, sensitivity and efficiency. In this paper the feasibility of LC/API-MS techniques in the identification, structure characterization and quantitation of drug metabolites is reviewed. Sample preparation, LC techniques, isotope labeling, suitability of different MS techniques, such as tandem mass spectrometry, and high-resolution MS in drug metabolite analysis, are summarized and discussed. Automation of data acquisition and interpretation, special techniques and possible future trends are also the topics of the review. Copyright © 2003 John Wiley & Sons, Ltd.

Published
2003
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41. Poly(dimethylsiloxane) electrospray devices fabricated with diamond-like carbon–poly(dimethylsiloxane) coated SU-8 masters

Author

Huikko, K., Östman, P., Grigoras, K., Tuomikoski, S., Tiainen, V.-M., Soininen, A., Puolanne, K., Manz, A., Franssila, S., Kostiainen, R., and Kotiaho, T.

Abstract

This study presents coupling of a poly(dimethylsiloxane) (PDMS) micro-chip with electrospray ionization-mass spectrometry (ESI-MS). Stable electrospray is generated directly from a PDMS micro-channel without pressure assistance. Hydrophobic PDMS aids the formation of a small Taylor cone in the ESI process and facilitates straightforward and low-cost batch production of the ESI-MS chips. PDMS chips were replicated with masters fabricated from SU-8 negative photoresist. A novel coating, an amorphous diamond-like carbon–poly(dimethylsiloxane) hybrid, deposited on the masters by the filtered pulsed plasma arc discharge technique, improved significantly the lifetime of the masters in PDMS replications. PDMS chip fabrication conditions were observed to affect the amount of background peaks in the MS spectra. With an optimized fabrication process (PDMS curing agent/silicone elastomer base ratio of 1/8 (w/w), curing at 70 °C for 48 h) low background spectra were recorded for the analytes. The performance of PDMS devices was examined in the ESI-MS analysis of some pharmaceutical compounds and amino acids.

Published
2003

47. Mikrokanavia biosiruihin

Author

Franssila, S., Kotiaho, T., Kostiainen, R., Baumann, M., Leppihalme, M., Ari Hokkanen, and Grigoras, K.

Abstract

Piikiekolle syövytetyillä mikrokanavilla voidaan ohjailla mikroskooppisia neste- ja kaasuvirtauksia kemiallisen analyysin tarpeisiin. Aikaansaadut mikrofluidistiikan komponentit ovat yleensä kahdesta piikiekosta liitettyjä kolmiulotteisia verkostoja. Niiden suunnittelu ja valmistus ovat kuitenkin räätälintyötä.

48. Mobility scale adjustment using 2,6-DtBP

Author

Anna-Kaisa Viitanen, Mauriala, T., Mattila, T., Adamov, A., Pedersen, C. S., Jyrki Mikael Mäkelä, Marjamäki, M., Sysoev, A., Jorma Keskinen, and Kotiaho, T.

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