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The datasets contain the results of the impact of five C-1748 derivatives (IKE28 - IKE32) on morphological transformation of Candida albicans strain ATCC 10231 cells. Photographic documentation prepared with the TECAN Spark 10M titration plate reader.

The datasets contain the results of the impact of five C-1748 derivatives (IKE28 - IKE32) on the biofilm formation of Candida albicans strain ATCC 10231 cells. Photographic documentation prepared with the TECAN Spark 10M titration plate reader.

The datasets contain the results of determining the minimum inhibitory concentration of C-1330 derivatives (IKE9-IKE14) against C. albicans ATCC 10231, C. glabrata ATCC 90030, C. krusei ATCC 6258 and C. parapsilosis ATCC 22019 by the modified M27-A3 specified by the CLSI.

The presented data complement the studies on the interplay between C-2028 (anticancer-active unsymmetrical bisacridine) and the glutathione S-transferase/glutathione (GST/GSH) system.

The principle of operation of the kit is based on the fact that the tested sample with endocrine activity (containing compounds capable of binding to the human estrogen receptor α, hERα) releases the fluorescently labeled ligand (FluormoneTM ES2) from the receptor-ligand complex, which results in a reduction of the polarization value fluorescence. The maximum value of fluorescence polarization is shown by the receptor-ligand complex, while the minimum mixture containing additionally 1 µM 17β-estradiol (the sex hormone with the highest biological activity).The endocrine potential of the tested samples was compared with the activity of estradiol by determining the endocrine equivalent concentration (EEQ).

The dataset contains the results of a single series of determinations of the antimicrobial properties against Listeria monocytogenes of the innovative CS-2 a preservative in the solution of model fluids.During the test, the infected product is inoculated (on Oxford Agar) at specified intervals (0 min. [Listeria cs2a 0] and after 1 day-24 h of incubation [Listeria cs2a 1]). This allows the degree of microbial reduction caused by the presence of the preservative in the sample to be determined.After the specified incubation period (48 h 37 ° C), plates containing, if possible, less than 300 colonies in a dilution series were selected for counting.

The dataset contains the results of a single series of determinations of the antimicrobial properties against E. coli of the innovative CS-2 b preservative in the solution of model fluids.During the test, the infected product is inoculated (on chromogenic Coliform Agar) at specified intervals (0 min. [cs2b 0] and after 1 day-24 h of incubation [cs2b 1]). This allows the degree of microbial reduction caused by the presence of the preservative in the sample to be determined.After the specified incubation period (48 h 37 ° C), plates containing, if possible, less than 300 colonies in a dilution series were selected for counting.

The dataset contains results determining the effect of zinc oxide addition at different concentrations on the dry matter content of chitosan gels in carbonic acid after a specified time. Zinc oxide at concentrations of 0.5 and 1% was added as a powder to a 1% solution of chitosan in carbonic acid and then homogenized. Differences were observed in the gelation time, which was significantly longer at the lower concentration of zinc oxide. Furthermore, the gel with 0.5% zinc oxide was less consistent compared to the gel with 1% zinc oxide added.

The rheological characteristic of pectin gel (with concetration 0,25% m/m, in two independent repetitions) wasmade on the basis of a viscosity measurement using a Brookfield viscometer, using LV SC4 - 27 spindle and shear rates from 1.7 to 85 s-1, in temperature of 25 C degee .The data allowed to characterize the used hydrogel preparations and assess their functional parameters as substances modifying the rheology of thickeners.

The dataset shows the change in the viscosity of a hydrogel medium over time. Viscometric measurement was used as a universal method for determining the cross-linking rate of chitosan hydrogel using metal oxides or other solutions with a negative net charge. The method was prepared as a method of predicting the kinetics of additive manufacturing using the bioprinting method dedicated to the production of cell scaffolds.

The dataset contains the results of a single series of determinations of the antimicrobial properties against Staphylococcus aureus of the innovative CS-2 a preservative in the solution of model fluids.During the test, the infected product is inoculated (on Baird-Parker Agar) at specified intervals (0 min. and after 1 day-24 h of incubation ). This allows the degree of microbial reduction caused by the presence of the preservative in the sample to be determined.After the specified incubation period (48 h 37 ° C), plates containing, if possible, less than 300 colonies in a dilution series were selected for counting.

The dataset includes the effect of flaxseed addition on the properties of toasted bread bakery products such as basic composition, moisture content immediately after baking and after a defined time, porosity, acidity and total baking loss. The following bread variants were investigated: control bread, bread with 8% and 12% flaxseed addition and competitive bread.

The dataset contains the results of Jerusalem artichoke addition on the texture of wheat bread. The following bread variants were tested: control bread, bread with 15% and 30% Jerusalem artichoke addition. Measurements were made immediately after baking. On the basis of data, the following parameters were determined: hardness, elasticity, cohesion and chewiness.

Inhibition of Yeast Topoisomerase II were analyzed according to relaxation assay kit from Inspiralis. Briefly, 250 ng of supercoiled pBR322 DNA, 1 mM ATP, 1-200 μM of analyzed compound were mixed with reaction buffer (1 mM Tris-HCl (pH 7.9), 10 mM KCl, 0.5 mM MgCl 2, 0.2 % (v/v) glycerol). The reaction was initiated by the addition of an enzyme, allowed to proceed at 30°C for 30 min and terminated by addition of 40% (w/v) sucrose, 100 mM Tris-HCl pH 8, 10 mM EDTA, 0.5 mg mL -1 Bromophenol Blue. Two step extraction with chloroform:isoamyl alcohol (24:1) and butanol water were made and mixtures were analyzed on the 1% agarose gel in 1x TAE buffer, 3h, 4.5 V cm -1. Gel was stained in GelRed 3x staining solution for 30 min and photographed with Gel Doc XR+Gel Documentation System.

The dataset includes the effect of wax foundation addition on the basic mechanical properties of the filaments. PCL and wax foundation addition at 10 and 15% were used for extrusion. The mechanical properties of the resulting filaments were evaluated by a double compression test using an Instron model 5543 universal testing machine. Parameters such as tensile strength, elongation and Young's modulus were determined from the raw data imported into a spreadsheet.

The dataset contains the results of flaxseed addition on the texture of toasted bread. The following bread variants were tested: control bread, bread with 8% and 12% linseed addition and competitive bread. Measurements were made immediately after baking and after 4 days. On the basis of data the following parameters were determined: hardness, elasticity, cohesion and chewiness.

The datasets contain the results of determining the minimum inhibitory concentration of imidazoacridinone derivatives against C. albicans ATCC 10231, C. glabrata ATCC 90030, C. krusei ATCC 6258 and C. parapsilosis ATCC 22019 and C. albicans clinical strains by the modified M27-A3 specified by the CLSI.

Concentration levels of five selected steroid hormones in influent and effluent waters from wastewater treatment plant were determined using SPE(C18)-HPLC-ESI/MS/MS method. Sex hormones like: estradiol, estriol, estrone, 17-α-ethynyloestradiol, progesterone were present in all influent wastwater samples.

The datasets contain the results of determining the effect of raw material type on endotoxin content as well as the effect of purification methods. The endotoxin concentration was determined using the PyroGene Recombinant Factor C Endpoint Fluorescent Assay. Beckmann-Kenko sodium alginate and Chemat sodium salt of alginic acid were used for the assays. The 1% solutions of alginate were used for the experiments, unpurified and purified using chloroform extraction before and after precipitation. A 0.7% solutions of chitosan in 0.1M glycolic acid from Chitone, Simga, Chin, Chemat and Chitinor were also used in this study. Purification of the solution from the endotoxin was by chloroform extraction and carbon dioxide saturation method. On the basis of raw data imported into a spreadsheet, parameters such as the concentration of endotoxins (EU/ml) in the raw materials and the effect of purification methods were determined.

The rheological characteristic of pectin gel (with concetration 0,45% m/m, in three independent repetition of sample) was made on the basis of a viscosity measurement using a Brookfield viscometer, using LV SC4 - 25 spindle and shear rates from 1.1 to 55 s-1.The data allowed to characterize the used hydrogel preparations and assess their functional parameters as substances modifying the rheology of thickeners.

Optical measurements of C-1305 derivatives (IKE1-IKE8). The absorption spectra were recorded at 300 - 800 nm for solutions with 16 μg / mL derivative concentration. The fluorescence emission spectra were determined at 420-800 nm with the excitation wavelengths 360 or 415 nm for solutions with 1 μg / mL derivative concentration. All measurements were recorded using a multiplate reader Tecan Spark 10M.

The dataset includes results to determine the effect of chitosan concentration and molecular weight on endotoxin content as well as the effect of purification methods. The endotoxin concentration was determined using the PyroGene Recombinant Factor C Endpoint Fluorescent Assay. Chitosan 0.6% in 0.1M glycolic acid from Chemat LMW, MMW, and HMW were used in this study. Also chitosan from Chemat MMW with the following concentrations of 0.2, 0.4, 0.6, and 0.8% purified by chloroform extraction with carbon dioxide saturation method has been used in the experiments. On the basis of raw data imported into a spreadsheet, parameters such as the concentration of endotoxins (EU/ml) in the raw materials and the effect of purification methods were determined.

The rheological characteristics of the pectin hydrogel modified by hydroxypropylcellulose (added in a concetration range from 0.1 to 0.55% m/m) were made on the basis of the viscosity assessment using a Brookfield viscometer with an LV SC4 - 25 spindle and a shear rate of 0,22 s-1 in the temperature range 19-96 ° C.The data allowed selection of optimal conditions for the technological process of manufacturing the final product.

Unsymmetrical bisacridines (UAs) are a novel potent class of antitumor-active therapeutics. The aim of this study was to investigate the possible role of human glutathione S-transferase M1-1 (hGSTM1-1) in the glutathione (GSH) conjugation of a representative UA, C‑2028.

The rheological characteristics of the high-fructose syrup modified stability of pectin (added in a concentration 2% m/m) were made on the basis of the viscosity assessment using a Brookfield viscometer with an LV SC4 - 25 spindle and a shear rate of 0,22 s-1 in the temperature range 25-96 ° C. The data allowed selection of optimal conditions for the technological process of manufacturing the final product.

Data set presents the results of monosaccharides and ethyl alcohol determination in samples of fermented cereal drink with increased health value based on barley malt and roasting of rye, chicory and beetroot, and with the addition of hops. The HPLC method with RID detector was used for determinations

The dataset contains the results of determination the effect of rhamnolipids concentration, calcium chloride concentration and ionic cross-linking time on the mechanical properties of alginate hydrogels prepared by immersing the alginate mixture limited by the dialysis membrane in an appropriate cross-linking solution containing calcium ions. The mechanical properties of the obtained hydrogels were assessed based on a double compression test with the use of a universal testing machine Instron model 5543. On the basis of raw data imported into a spreadsheet, parameters such as hardness, flexibility and cohesion were determined.

The rheological characteristics of poly(acrylic acid) PAA solutions were based on viscosity measurements using a Brookfield viscometer, using LV SC4 - 27 spindles and shear rates from 1,7 to 39 s-1 (Fig. 1). PAA solutions with were prepared in water. The data allowed to characterize the used PAA and assess their functional parameters as rheology modifying substances of adhesive.

The dataset contains the results of a single series of determinations of the antimicrobial properties against E. coli and S. aureus of polyethylene films containing the nanozinc filler.

The rheological characteristics of hydrogel were made on the basis of a viscosity measurement using a Brookfield viscometer, using LV SC4 - 27 spindle and shear rates from 1.7 to 34 s-1.

The video presents instructional work on the production of un-matured white soft cheese.

Municipal waste waters are one of the main sources of estrogenic compounds in aquatic environments. Feminization of fish downstream of Waste Water Treatment Plants (WWTPs) discharges has been observed worldwide. Some estrogenic chemicals, particularly steroid estrogens, are known to cause disruption of the endocrine system of fishes and abnormalities of the reproductive tract in ng/L concentrations, which commonly occur in aquatic environment worldwide.

The rheological characteristics of hydrogel were made on the basis of a viscosity measurement using a Brookfield viscometer, using LV SC4 - 25 spindle and shear rates from 1.1 to 55 s-1.

The rheological characteristics of the refined oil were made on the basis of the viscosity assessment using a Brookfield viscometer with an LV SC4 - 18 spindle and a shear rate of 52.8 s-1 in from temperature 19-96 °C.

The profiling of tannic acid is determined by examining the content and proportion / ratio of gallotannins [Aras et al. 2016, Clifford et al. 2007, Gauri et al. 2012, Nishizawa et al. 1982]. In order to check the ion mass distribution (TIC) in an aqueous solution of commercial tannic acid, a direct sample injection (TA) was performed into the mass spectrometer. Then, to obtain the chromatographic profile, the sample was analyzed by HPLC combination with a DAD and MS (-ESI) SCAN detectors. The compounds characteristic of the MS SCAN (ESI-) spectrum of tannic acid are a combination of various intermediate forms: galloyl glucoses and galloylquinic acid. The values ​​(m/z) [M-H] - of ions in the MS spectras (TIC) correspond to the following compounds: 169 - gallic acid, 321 - digallic acid, 343 - galloylquinic acid, 483 - digalloyl glucose, 495 - digalloylquinic acid 635 - trigalloyl glucose, 647 - trigalloylquinic acid, 787 - tetragalloyl glucose, 799 - trigalloylquinic acid 939 - pentagalloyl glucose, 1091 - hexagalloyl glucose, 1243 - heptagalloyl glucose, 1396 - octagalloyl glucose.

The rheological characteristics of hydrogel were made on the basis of a viscosity measurement using a Brookfield viscometer, using LV SC4 - 18 spindle and shear rates from 6.6 to 330 s-1.

Oak gall−nuts are pathological excrescences on the leaves of oaks (in Poland: Quercus robur L. and Q. petraea Liebl.) created by the Cynips quercusfolii L. The potential medical use of these non−wood forest products should be taken under consideration because of widespread use in folk medicine, but also because of the presence of gall−nuts of Middle Eastern oak species Quercus infectoria in the current Farmakopea… [2017]. The aim of this study was the preliminary analyzes of Quercus robur L. galls in the context of the diversity of the raw material and its possible consequences for the variability in tannin content, and therefore for the therapeutic value. The galls were collected in September 2018 from five living trees forming compact mid−field tree plantation in Podkarpackie Region (SE Poland). To determine the tannin content, hide powder method was used, while mass spectrometry was used for determining the identity of the raw material. The tannin content was found to be between 10.02 ±0.2% and 15.47 ±0.07%. There were no statistical differences between the tannin content of four weight groups of tested gall−nuts. The analysis of mass spectra showed the presence of gallic acid and tannin disintegration products. The results indicate that the tannin content in domestic oak galls is lower than in Quercus infectoria ones, for which less than 20% is required, however Polish galls are still the richest native tannin raw material. It was not proved that the content of tannins in oak galls was influenced by their size. A large variation in tannin content depending on the sample suggests that it might be possible to identify i.e. forest sites or climatic zones, where the obtained gall−nuts will be characterized by the high tannin content., Sylwan 163 (9): 746-753