141 results on '"Kozarski, Maja"'
Search Results
2. Mushroom β-glucan and polyphenol formulations as natural immunity boosters and balancers: nature of the application
- Author
-
Kozarski, Maja, Klaus, Anita, van Griensven, Leo, Jakovljevic, Dragica, Todorovic, Nina, Wan-Mohtar, Wan Abd Al Qadr Imad, and Vunduk, Jovana
- Published
- 2023
- Full Text
- View/download PDF
3. Oxidative stress prevention by edible mushrooms and their role in cellular longevity
- Author
-
Kozarski, Maja, primary and van Griensven, Leo J. L. D., additional
- Published
- 2022
- Full Text
- View/download PDF
4. Wine is more than just a beverage: Chemical diversity, health benefits, and immunomodulating potential of wine polyphenols
- Author
-
Mitrović, Danka, primary, Sredović Ignjatović, Ivana, additional, Kozarski, Maja, additional, and Popović‐ Đorđević, Jelena, additional
- Published
- 2024
- Full Text
- View/download PDF
5. Bioprospecting of Selected Species of Polypore Fungi from the Western Balkans
- Author
-
Kozarski, Maja, primary, Klaus, Anita, additional, Špirović-Trifunović, Bojana, additional, Miletić, Srdjan, additional, Lazić, Vesna, additional, Žižak, Željko, additional, and Vunduk, Jovana, additional
- Published
- 2024
- Full Text
- View/download PDF
6. Effect of modified atmosphere packaging on selected functional characteristics of Agaricus bisporus
- Author
-
Vunduk, Jovana, Kozarski, Maja, Djekic, Ilija, Tomašević, Igor, and Klaus, Anita
- Published
- 2021
- Full Text
- View/download PDF
7. Impact of grape pomace as a cultivation substrate on the Pleurotus ostreatus chemical and biological properties
- Author
-
Doroški Ana, Klaus Anita, Kozarski Maja, Nikolić Biljana, Vunduk Jovana, Lazić Vesna, and Đekić Ilija
- Subjects
pleurotus ostreatus ,food waste ,quality index ,grape pomace ,mushroom ,Technology (General) ,T1-995 - Abstract
The objective of this study was to develop a single quality index of chemical characteristics of Pleurotus ostreatus extracts on 7th and 14th day of its shelf life, derived from the mushroom fruiting bodies. P. ostreatus was cultivated on four substrates containing different ratio of wine industry waste-grape pomace (P) and wheat straw (S): 100P, 80P20S, 50P50S, 20P80S. Four quality parameters of P. ostreatus mushroom extracts, i.e. antioxidative parameters: ABTS+ and DPPH• free radical scavenging capability, total phenolic compounds (TPC) and total polysaccharides (TPS) were used to define the final extract quality index. Analysis indicated 100P and 80P20S as the samples cultivated on the substrate with higher percent of grape pomace, as the best quality at the 7th day of its shelf life. On the other hand, final quality score indicated 50P50S and 20P80S, cultivated on a substrate with a lower percent of grape pomace, as the best quality samples at the 14th day of its shelf life. According to the results, samples cultivated on a higher pomace content substrate are of better quality in a shorter storage time period.
- Published
- 2021
- Full Text
- View/download PDF
8. Identifying the Biological Potential of Western Balkan Polypore Mushroom Species to Mitigate the Negative Effects of Global Mushroom Cultivation
- Author
-
Kozarski, Maja, primary, Klaus, Anita, additional, Špirović-Trifunović, Bojana, additional, Miletić, Srdjan, additional, Lazić, Vesna, additional, Žižak, Željko, additional, and Vunduk, Jovana, additional
- Published
- 2023
- Full Text
- View/download PDF
9. The influence of mushroom Coriolus versicolor and hazelnuts enrichment on antioxidant activities and bioactive content of dark chocolate
- Author
-
Kozarski Maja S., Klaus Anita S., Vunduk Jovana Đ., and Nikšić Miomir P.
- Subjects
antioxidant potency composite index ,dark chocolate ,enriched dark chocolate products ,Food processing and manufacture ,TP368-456 - Abstract
Oxidative stress is among the main culprits for the progression of chronic diseases such as cardiovascular diseases, diabetes, and neurodegenerative disorders. It is well known that dark chocolate possesses polyphenols as major constituents which dietary consumption has been associated to health beneficial effects. Consequently, this study aimed to analyze dark chocolate (DC) and dark chocolate enriched with medicinal mushroom Coriolus versicolor (DCC) and hazelnuts (DCH) regarding antioxidant potential and total polyphenol (TPC), flavonoid (TFC), flavan-3-ol (FLA) and proanthocyanidin (PCA) content. DPPH•, CUPRAC and ABTS•+ assays were applied for measuring antioxidant capacity. The average of all antioxidant tests for each product was used for calculating the antioxidant potency composite index (ACI). The mean values of all antioxidant assays indicated that all chocolate products contained potent antioxidants. The addition of hazelnuts to dark chocolate significantly affected its total flavonoid content. Regression analysis among results obtained with antioxidant assays revealed that dark chocolate and dark chocolate products may act in mixed mode, by direct reduction via electron transfer or by radical quenching via H atom transfer. Flavan-3ols had the most significant impact on the ability of the analyzed samples to reduce metals, while proanthocyanidins primarily acted as radical scavengers. The obtained results provided additional information regarding the value-added dark chocolates enriched with bioactive compounds of medicinal mushroom and plant origin.
- Published
- 2020
10. Health impact of the commercially cultivated mushroom Agaricus bisporus and wild-growing mushroom Ganoderma resinaceum - a comparative overview
- Author
-
Kozarski Maja S., Klaus Anita S., Vunduk Jovana Đ., Jakovljević Dragica M., Jadranin Milka B., and Nikšić Miomir P.
- Subjects
hot water extracts ,cytotoxicity ,enzyme inhibition ,lipid peroxidation ,Chemistry ,QD1-999 - Abstract
The health promoting effects of hot water extracts obtained from fruiting bodies of the commercially cultivated mushroom Agaricus bisporus (AbHW) and the wild-growing mushroom Ganoderma resinaceum (GrHW) originating from northern Serbia are presented in this research. These abilities were compared in vitro by the prevention of lipid peroxidation (LPx) in a linoleic acid model system, inhibition of the angiotension converting I enzyme (ACE) that could help in the maintenance of a normal blood pressure level and strengthening the ability of the central cholinergic neuron by inhibiting the activity of acetylcholinesterase (AChE). Cytotoxic activities were observed towards selected human malignant (HeLa and K562) cell lines and normal- -human peripheral blood mononuclear cells (PBMC). GrHW contains higher phenolics (5.9 g (100 g)-1), inhibition of LPx (EC50 = 1.07 mg mL-1), ACE (IC50 = 0.54 mg mL-1) and AChE (IC50 = 0.37 mg mL-1), and exhibited a significant selectivity in the antitumour action against HeLa (IC50 = 0.14 mg mL-1) and K562 (IC50 = 0.11 mg mL-1) cells. AbHW contained higher total protein (6.4 g (100 g)-1), carbohydrate (75.4 g (100 g)-1) and β-glucan (55.1 g (100 g)-1) contents and induced significant proliferation of healthy PBMC from 152–116 % in the concentration range of 0.047–0.187 mg mL-1. The difference in the biological activity of the extracts provides guidance on their use as functional food. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. III 46001, Grant no. III 46010 and Grant no. III 43004]
- Published
- 2020
- Full Text
- View/download PDF
11. Insight into Bioactive Compounds, Antioxidant and Anti-Diabetic Properties of Rosehip (Rosa canina L.)-Based Tisanes with Addition of Hibiscus Flowers (Hibiscus sabdariffa L.) and Saffron (Crocus sativus L.).
- Author
-
Vasić, Dušan, Katanić Stanković, Jelena S., Urošević, Tijana, Kozarski, Maja, Naumovski, Nenad, Khan, Haroon, and Popović-Djordjević, Jelena
- Subjects
ROSELLE ,SAFFRON crocus ,HERBAL teas ,HIBISCUS ,BIOACTIVE compounds - Abstract
Tisane is a fruit or herbal infusion, commonly referred to as herbal tea. These products are consumed as part of a balanced diet, which is closely related to the trend of a healthier lifestyle. In this work, tisanes prepared from rosehip (R), and herbal mixtures containing rosehip/hibiscus flowers (R/H) and rosehip/hibiscus flowers/saffron (R/H/S) were studied. Rosehip was dried by the convective drying method at 40, 50 and 60 °C. Total phenolic content (TPC), total flavonoid content (TFC), total flavonol content (TFlC), total anthocyanin content (TAC), antioxidant properties (DPPH
∙ and ABTS∙+ assays) and in vitro inhibitory potential toward α-amylase of tisanes were examined. The highest TPC (based on dry weight (dw)) was measured in tisane obtained from rosehip dried at 60 °C (37.84 mg GAE/g dw). Tisanes prepared from a R/H/S mixture had the highest values of TFC (4.66–6.13 mg QUE/g dw), TFlC (2.67–3.98 mg QUE/g dw) and TAC (1.35–2.27 mg Cy 3-glc/g dw). The highest DPPH∙ scavenging activity (53.42 mg TE/g dw) was measured in rosehip (dried at 60 °C) tisane, whereas tisane prepared from a rosehip (dried at 60 °C)/hibiscus mixture expressed the best ABTS∙+ scavenging activity (107.44 mg TE/g dw). All tisane samples expressed high inhibitory potential toward α-amylase, with the highest activity of 85.03% and 89.90%, measured for tisanes prepared from rosehip/hibiscus flowers mixture (rosehip dried at 50 and 60 °C, respectively). [ABSTRACT FROM AUTHOR]- Published
- 2024
- Full Text
- View/download PDF
12. The Effect of Green Extraction Technologies on the Chemical Composition of Medicinal Chaga Mushroom Extracts.
- Author
-
Lazić, Vesna, Klaus, Anita, Kozarski, Maja, Doroški, Ana, Tosti, Tomislav, Simić, Siniša, and Vunduk, Jovana
- Subjects
INDUSTRIAL chemistry ,GLUCANS ,POLYSACCHARIDES ,PHENOLS ,CHLOROGENIC acid ,EXTRACTION techniques ,MUSHROOMS ,ETHANOL - Abstract
The mushroom industry should implement green extraction technologies; however, there is not enough information on the differences between these techniques expressed as the chemical composition of the resulting extract. In this study, selected types of green extraction techniques (GETs) were used on Chaga (Inonotus obliquus) (Fr.) Pilát from Serbia (IS) and Mongolia (IM) to examine the differences that would enable the composition-based technology choices in the mushroom supplement industry. Subcritical water extraction (SWE), microwave-assisted (MW) extraction, and ultrasonic-assisted extraction (VAE) were used to prepare the extracts. SWE was performed at two different temperatures (120 and 200 °C), while 96% ethanol, 50% ethanol, and water were used for MW and VAE. The yield, the content of total phenols, total proteins, and carbohydrates, qualitative and quantitative analysis of phenolic compounds, carbohydrates, including α- and β- and total glucans, and fatty acids, were determined in the obtained extracts. SWE resulted in a significantly higher yield, total polysaccharide, and glucan content than any other technique. Glucose was the most dominant monosaccharide in the SWE samples, especially those extracted at 200 °C. The MW 50% EtOH extracts showed the highest yield of total phenols. Among the tested phenolic compounds, chlorogenic acid was the most dominant. SWE can be recommended as the most efficient method for extracting commercially important compounds, especially glucans and phenols. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
13. Ganoderma lucidumas a cosmeceutical: Antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes
- Author
-
Kozarski Maja, Klaus Anita, Jakovljević Dragica, Todorović Nina, Wan-Mohtar Wan Abd Al Qadr Imad, and Nikšić Miomir
- Subjects
Ganoderma lucidum ,cosmeceutical ,maturate skin ,oxidative stress ,polysaccharide extracts ,Biology (General) ,QH301-705.5 - Abstract
Three different polysaccharide extracts of a wild European source of the mushroom Ganoderma lucidum were screened for their free radical-blocking potential, which could strengthen the skin’s barrier function, and provide a skinlightening effect via potential inhibition of tyrosinase. The anti-collagenase and anti-elastase activities, which can help to restore skin elasticity and tensile strength, were also evaluated for the three extracts. Carbohydrates were the most abundant components of the extracts, followed by smaller quantities of proteins, phenols and flavonoids. The glucan fraction represented between 48% and 61% of carbohydrate content in all three extracts. None of the analyzed extracts showed overt toxicity to spontaneously-transformed immortal human keratinocytes (HaCaT) at concentrations up to 2 mg/mL, and displayed superior scavenging ability on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl- 2-picrylhydrazyl (DPPH) radicals. The hot water crude polysaccharide extract (HWCP) and partially purified fraction (HWPP) were found to be effective inhibitors of lipid peroxidation (LPx), with an almost two-fold increased inhibition of LPx compared with ascorbic acid (EC50=1.65Ѓ}0.08 mg/mL), a common additive in cosmeceutical formulations and used at mg levels. Among the investigated extracts, HWCP showed the strongest inhibition potential on tyrosinase and skin extracellular matrix (ECM) degradation enzymes. These diverse functionalities indicate that G. lucidum may represent a promising source of natural cosmeceutical ingredients. [Projects of the Serbian Ministry of Education, Science and Technological Development, Grant no. III 46001, Grant no. III 46010 and Grant no. III 43004]
- Published
- 2019
- Full Text
- View/download PDF
14. The impact of puffball autolysis on selected chemical and biological properties: Puffball extracts as potential ingredients of skin-care products
- Author
-
Petrović Predrag, Ivanović Katarina, Jovanović Aleksandra, Simović Milica, Milutinović Violeta, Kozarski Maja, Petković Miloš, Cvetković Anka, Klaus Anita, and Bugarski Branko
- Subjects
puffballs ,mushrooms ,tyrosinase inhibitory activity ,antioxidant ,cosmeceutical ,Biology (General) ,QH301-705.5 - Abstract
Puffballs are fungi that produce globose fruiting bodies that undergo autolysis, transforming their insides into a spore bearing, powdery mass. Mature fruiting bodies are traditionally used to treat open skin wounds. In this study, methanol extracts of two puffball species, Handkea excipuliformis and Vascellum pratense, were examined and compared in order to provide insight into the changes these mushrooms undergo during maturation, with respect to their potential use in skin care and wound treatment. Some compounds involved in skin care and regeneration were quantified, and it was found that maturation increases the concentrations of almost all of these compounds. Antioxidant activity was also more pronounced in mature fruiting body extracts, which was in correlation with the higher content of antioxidants. Tyrosinase inhibition was vastly improved with autolysis, correlating with the higher phenolic content in mature fruiting body extracts. Antimicrobial activity was negatively affected by autolysis in the case of H. excipuliformis, whereas autolysis had little effect on the antimicrobial activity of V. pratense. Autolysis generally improved the biological activity and increased the concentrations of compounds involved in skin care, which justifies the traditional use of puffballs and makes them good candidates for various potential cosmetic and medicinal skin-care products. [Project of the Serbian Ministry of Education, Science and Technological Development, Grant no. III 46010]
- Published
- 2019
- Full Text
- View/download PDF
15. Challenging the difference between white and brown Agaricus bisporus mushrooms : Science behind consumers choice
- Author
-
Vunduk, Jovana, Djekic, Ilija, Petrović, Predrag, Tomašević, Igor, Kozarski, Maja, Despotović, Saša, Nikšić, Miomir, and Klaus, Anita
- Published
- 2018
- Full Text
- View/download PDF
16. Artificial Neural Network Prediction of Antiadhesion and Antibiofilm-Forming Effects of Antimicrobial Active Mushroom Extracts on Food-Borne Pathogens
- Author
-
Vunduk, Jovana, primary, Klaus, Anita, additional, Lazić, Vesna, additional, Kozarski, Maja, additional, Radić, Danka, additional, Šovljanski, Olja, additional, and Pezo, Lato, additional
- Published
- 2023
- Full Text
- View/download PDF
17. Biological potential of puffballs: A comparative analysis
- Author
-
Petrović, Predrag, Vunduk, Jovana, Klaus, Anita, Kozarski, Maja, Nikšić, Miomir, Žižak, Željko, Vuković, Nebojša, Šekularac, Gavrilo, Drmanić, Saša, and Bugarski, Branko
- Published
- 2016
- Full Text
- View/download PDF
18. Polysaccharides of higher fungi: Biological role, structure, and antioxidative activity
- Author
-
Kozarski Maja S., Klaus Anita S., Nikšić Miomir P., van Griensven Leo J.L.D., Vrvić Miroslav M., and Jakovljević Dragica M.
- Subjects
polysaccharides ,fungi ,polysaccharide extracts ,antioxidative properties ,Chemical technology ,TP1-1185 - Abstract
Fungal polysaccharides attract a lot of attention due to their multiple challenging biological properties, such as: anti-tumor, anti-viral, anticomplementary, anticoagulant, hypolipidemic and immunomodulatory and immune-stimulatory activities, which all together make them suitable for application in many quite distinctive areas, such as food industry, biomedicine, cosmetology, agriculture, environmental protection and waste water management. This article presents results with respect to biological properties, structure and procedures related to the isolation and activation of polysaccharides of higher fungi. It is considered and presented along with a review of the critical antioxidative activity and possible influence of the structural composition of polysaccharide extracts (isolated from these higher fungi) upon their antioxidative properties.
- Published
- 2014
- Full Text
- View/download PDF
19. Zeolites as possible biofortifiers in Maitake cultivation
- Author
-
Vunduk Jovana, Klaus Anita, Kozarski Maja, Đorđević R., Jovanović Lj., and Nikšić M.
- Subjects
Grifola frondosa ,Maitake mushroom ,biofortification ,zeolite ,Minazel Plus ,ICP-OES ,Biology (General) ,QH301-705.5 - Abstract
The levels of Ni, Cu and Mg in Grifola frondosa (also known as Maitake mushroom) fruit body produced on zeolite Minazel Plus (MG)-supplemented substrate were measured with inductively coupled plasma optical emission spectrometry (ICP-OES). Two different concentrations of MG were added to the substrate for mushroom cultivation. Levels of selected metals were measured in cultivated dry carpophores. The content of Ni increased in fruit bodies produced on supplemented substrate, while in case of Cu, a pronounced decrease was observed. When two different concentrations of MG were implemented, the Mg level showed both positive and negative trend, depending on the applied concentration of zeolite. MG in a concentration of 1% showed the strongest influence on the observed elements in the cultivated fruiting body of Maitake mushroom. [Projekat Ministarstva nauke Republike Srbije, br. 46010]
- Published
- 2014
- Full Text
- View/download PDF
20. Lignicolous mushroom Fomitopsis pinicola as a potent inhibitor of lipid peroxidation
- Author
-
Kozarski, Maja, Klaus, Anita, Vunduk, Jovana, Lazić, Vesna, Spirović Trifunović, Bojana, Miletić, Srđan, Spasić, Snežana, and Jakovljević, Dragica
- Subjects
functional food ,antioxidant potential ,lipid peroxidation ,phenol profile ,lignicolus mushroom - Abstract
Excessive production of reactive oxygen species (ROS) often results in irreversible cell damage and leads to a number of disorders such as hypertension, heart disease, diabetes, inflammation, premature aging and cancer. Considering the importance of diet in prevention of diseases associated with oxidative stress, this study was conducted to evaluate antioxidant activity of the methanol extract of wild lignicolus mushroom Fomitopsis pinicola as natural source of functional food ingredients. Nowadays functional food products based on the lignicolous mushroom species are increasingly available on the market. Their powders and extracts are consumed as dietary supplements in the form of capsules or tablets and as additives in the formulation of healthier food products.
- Published
- 2022
21. Extract from wild strain of mushroom Ganoderma lucidum as natural antioxidant
- Author
-
Kozarski Maja S., Klaus Anita S., and Nikšić Miomir P.
- Subjects
antioxidant properties ,Ganoderma lucidum ,polysaccharide extract ,Science (General) ,Q1-390 - Abstract
Recently, much attention has been paid to revealing natural biomaterials for clinical purposes since use of synthetic antioxidants is restricted due to their carcinogenicity. Among various natural antioxidants, polysaccharides, in general, have strong antioxidant activities and can be explored as novel potential antioxidants. The aim of this work was to examine the antioxidant properties of hot water extracted polysaccharides from Ganoderma lucidum in the form of mature fruit bodies, collected from the Bojčinska forest near Belgrade, the Republic of Serbia. Antioxidant properties were assayed in vitro, by the conjugated diene method, reducing power, scavenging abilities against 1.1-diphenyl-2-picrylhydrazyl (DPPH) radicals and chelating ability on ferrous ions. At concentrations of 1 mg/ml, the scavenging ability of G. lucidum polysaccharide extract on DPPH radicals was 74.7 %. At 1 mg/ml, the radical scavenging ability of the positive controls BHT, ascorbic acid and α-tocopherol were 11.5, 77.1 and 79.4 %, respectively. The antioxidant activity of the polysaccharide extract increased as the concentration increased to 78.0% at 20 mg/ml. Antioxidant activities of ascorbic acid and α-tocopherol were 63.8 % and 65.4% at 20 mg/ml. Polysaccharide extract from G. lucidum showed steady increase in the reducing activity as concentrations increased to 2.9 at 20 mg/ml. Ascorbic acid, used as a positive control, had a reducing power of 3.9 at 5 mg/ml. Chelating effects of the polysaccharide extract on ferrous ion increased with the increased concentrations. At 0.1-20 mg/ml, the chelating ability of G. lucidum polysaccharide extract was between 10.3-87.8%. The chelating effect of the synthetic metal chelator EDTA was 100% at 0.1-20 mg/ml, while citric acid did not prove to be good chelating agent for ferrous ions in this assay since its chelating ability was 10.3% at 20 mg/ml.
- Published
- 2011
- Full Text
- View/download PDF
22. Antioxidant properties of hot water extracts from carpophore and spores of mushroom Ganoderma lucidum
- Author
-
Klaus Anita S., Kozarski Maja S., and Nikšić Miomir P.
- Subjects
Ganoderma lucidum ,antioxidant activity ,scavenging effect ,reducing power ,chelating effect ,polysaccharide ,mushroom extract ,Science (General) ,Q1-390 - Abstract
Ganoderma lucidum (Leyss.:Fr.) Karst is one of the medicinal mushrooms, which possesses enviable antioxidant properties. Objective of this investigation was to evaluate antioxidant activity, reducing power, scavenging abilities on 1.1-diphenyl-2-picrylhydrazyl (DPPH) radicals and chelating effects on ferrous ions of hot water extracts obtained from carpophore and spores of this mushroom. Hot water extract from carpophore (Gl-I) showed high antioxidant activity of 85.7 ± 0.7%, at 10 mg/ml, while antioxidant activity of hot water extract from spores (Gl-Is) was 9.2 ± 0.3% at 10 mg/ml. Reducing power of Gl-I reached a plateau of 3.4 ± 0.1 at 20 mg/ml, and 0.3 ± 0.0 at 20 mg/ml for Gl-Is. At 10 mg/ml, scavenging ability on DPPH radicals of Gl-I increased to 96.8 ± 2.5%, whereas Gl-Is scavenged DPPH radicals by 69.6 ± 2.5% at 10 mg/ml. Gl-I chelated 81.6 ± 3.6 % of ferrous ions at 20 mg/ml, while the chelating effect of Gl-Is was 73.8 ± 1.7%. The antioxidative activities of hot water extracts from carpophore and spores of the mushroom G. lucidum were concentration dependent and increased with an increase in the concentration.
- Published
- 2011
- Full Text
- View/download PDF
23. Antioxidant activity of water extracts from fruit body of Lentinus edodes enriched with selenium
- Author
-
Savić Milena D., Klaus Anita S., Kozarski Maja S., and Nikšić Miomir P.
- Subjects
Antioxidant activity ,Extract ,Selenium ,Shiitake ,Science (General) ,Q1-390 - Abstract
Shiitake (Lentinus edodes) belongs to medically important and delicious fungi. It is recognizable for its healing properties, excellent taste and rich aroma. According to the traditional Japanese and Chinese medicine, shiitake mushroom significantly increases the strength and vitality of the body. Shiitake contains immunostimulants, compounds that lower cholesterol, prevents clogging of blood vessels, regulates the pressure, balances blood sugar levels, regulates digestion, and improves the performance of respiratory organs by its antirheumatic and antiallergic activities. Shiitake is recommended to use as food, prevention and cure, usually in a form of a spice (dried and ground) or tea. It can be consumed fresh, too. The objective of this study was to test the effect of enrichment in selenium on antioxidant, reducing and free radical scavenging activity of water extracts from fruit body of Lentinus edodes. The fungus was enhanced by adding organic selenium, zinc (II) complex with the ligand 2.6-bis diacetylpyridine (selenosemicarbazon) and inorganic compounds (Na2SeO3) of selenium in nutritional substrate where the fungus was grown. The total selenium content in fruit body was around 50 ppm for the sample enriched with selenium originating from organic sources, and 80 ppm for the sample enriched with selenium from inorganic sources. Samples were prepared by extraction of fruiting bodies in heated water. The results indicated that water extracts of whole fruit bodies, from both control and mushrooms supplemented with selenium, had quite good antioxidant activity. However, there was no significant difference between the samples supplemented with selenium content and those that were not.
- Published
- 2011
- Full Text
- View/download PDF
24. Antioxidative and immunomodulating activities of polysaccharide extracts of the medicinal mushrooms Agaricus bisporus, Agaricus brasiliensis, Ganoderma lucidum and Phellinus linteus
- Author
-
Kozarski, Maja, Klaus, Anita, Niksic, Miomir, Jakovljevic, Dragica, Helsper, Johannes P.F.G., and Van Griensven, Leo J.L.D.
- Published
- 2011
- Full Text
- View/download PDF
25. Influence of bioactive compounds extracted from mushroom Ganoderma lucidum on B and T cells
- Author
-
Klaus Anita S., Kozarski Maja S., and Nikšić Miomir P.
- Subjects
b-glucans ,bioactive polysaccharides ,Ganoderma lucidum ,Jurkatcells ,JY cells ,Science (General) ,Q1-390 - Abstract
Ganoderma lucidum (Leyss.: Fr.) Karst is one of the most often used mushrooms in traditional medicine of Far Eastern people. Because of its bitter taste and wooden build it is not suitable for nutrition, but the bioactive substances extracted from this mushroom possess very important medicinal characteristics. The aim of this experiment was to investigate the effects of different concentrations of isolated Ganoderma lucidum GL-I extract on the growth of JY (B) and Jurkat (T) cells. Obtained extracts were added to the cells in concentrations 1 mg/ml, 100 ng/ml, 10 ng/ml, 1 ng/ml and 100 pg/ml. JY and Jurkat cells were exposed to the action of bioactive compounds, b-glucans, during the incubation period of 72 h, at 37°C, in the atmosphere with 5% CO2 and their number was counted. Among all tested concentrations of extract the most important influence showed concentration of 1 mg/ml, which reduced the number of B cells by 61.46%, while in the case of T cells their number was reduced by 57.14%.
- Published
- 2009
- Full Text
- View/download PDF
26. Influence of structural features on immunostimulating activity of glucans extracted from Agaricus blazei mushroom
- Author
-
Kozarski Maja S., Klaus Anita S., and Nikšić Miomir P.
- Subjects
Agaricus blazei ,b-D-glucans ,IFN-g ,immunostimulating activity ,PBMC's ,Science (General) ,Q1-390 - Abstract
High molecular weight b-D-glucans derived from Basidiomycetes cell walls are able to specifically activate cellular and humoral components of the host immune system. The aim of this paper was to examine immunomodulating activity of native, chemically and enzimatically modified glucans from Agaricus blazei mushroom and to determine which structural features are of primary importance for their stimulation referring to humane immune cells. The immunomodulating activities were tested in vitro, by stimulation of peripheral blood mononuclear cells (PBMCs) and measuring of interferon-gamma (IFN-g) production by enzyme linked immunosorbent assay (ELISA). Measurements of immunomodulatory capacity of Agaricus blazei native glucans showed their expressive immunostimulating effect on activated PBMCs and synthesis of IFN-g. The results obtained after the stimulation of cells with 1M H2SO4 and 1M NaOH, the treated glucans showed that primary structure is of more importance than the tertiary structure of the triple helix for their immunostimulating activity and synthesis of IFN-g. Glucans of lower molecular weight obtained after acid hydrolysis appeared as effective immunostimulators of PBMC's. The results obtained after the incubation of cells with 1,6 b-glucanase modified glucans suggest that b-(1,6) binding of glucose monomers probably has no importance for the production of imunostimulating effects, in vitro. This confirmed that b-(1,3) bonds are the primary determinants of immunomodulatory activities and stimulation of IFN-g synthesis.
- Published
- 2009
- Full Text
- View/download PDF
27. Changes of quality and free radical scavenging activity of strawberry and raspberry frozen under different conditions
- Author
-
Stevanović, Snežana M., primary, Petrović, Tanja S., additional, Marković, Dragan D., additional, Milovančević, Uroš M., additional, Stevanović, Simo V., additional, Urošević, Tijana M., additional, and Kozarski, Maja S., additional
- Published
- 2021
- Full Text
- View/download PDF
28. Changes of quality and free radical scavenging activity of strawberry and raspberry frozen under different conditions.
- Author
-
Stevanović, Snežana M., Petrović, Tanja S., Marković, Dragan D., Milovančević, Uroš M., Stevanović, Simo V., Urošević, Tijana M., and Kozarski, Maja S.
- Subjects
RASPBERRIES ,BERRIES ,FREE radicals ,FROZEN foods industry ,STRAWBERRIES ,FROZEN fruit ,FRUIT industry - Abstract
The effects of different freezing methods and long‐term frozen storage on quality of strawberry and raspberry were investigated, that is, the retention of initial property and free radical scavenging (AO) activity after individual quick freezing (IQF) and conventional (discontinuous) freezing. The physicochemical properties, vitamin C, and AO activity were more affected by long‐term frozen storage than by freezing itself; however, their better retention was obtained after IQF. Moreover, AO activity was found to be higher in frozen samples compared to fresh fruits, but significant decrease was detected after 8 months of frozen storage. Despite the significantly higher initial content of vitamin C in strawberry, it demonstrated a lower AO activity than raspberry. Sensory attributes were significantly affected by freezing; the most considerable changes were recorded on their texture/firmness, especially after conventional (slow) freezing. Therefore, IQF can generally be suggested as a more suitable method of preserving these delicate fruits than slow freezing. Practical applications: Nowadays, consumers are increasingly interested in foods that possess not only good nutritional value but also health‐promoting property. Berries are known as rich sources of micronutrients and antioxidant compounds, but consumption of fresh strawberry and raspberry fruits may be limited because of seasonal and market availability; therefore, they must be preserved commonly by freezing. Frozen food industry is of crucial importance for developing countries, in which the fast freezing industry is rapidly developed. Investigation of the effect of industrial freezing and frozen storage on retention of the quality‐related property and antioxidant activity is important for providing the best quality for consumers and health benefits, as well as international fruit trade. In addition, the results obtained in this study could provide best practice and guidance for the frozen fruit industry. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
29. Mushroom β-glucan and polyphenol formulations as natural immunityboosters and balancers:nature of the application
- Author
-
Kozarski, Maja, Klaus, Anita, van Griensven, Leo, Jakovljevic, Dragica, Todorovic, Nina, Wan-Mohtar, Wan Abd Al Qadr Imad, and Vunduk, Jovana
- Abstract
Mushrooms are experiencing a kind of renaissance as a part of the contemporary human diet. These valuable organisms are more than food, they fit in perfectly as a novel market group known as nutra-mycoceuticals. Immune-balancing mushroom dietary fibers and secondary metabolites such as polyphenols are the main focus of the healthcare industry. Wellness and cosmetic companies are increasingly using mushroom extracts rich in these ingredients. This review considers the basic molecular immunomodulatory mechanisms of action of the most commonly used mushroom dietary fibers, β-glucans. The literature data on their bioavailability, metabolic transformations, preclinical and human clinical research, and safety are discussed. Immunomodulatory mechanisms of polyphenol ingredients are also considered. These molecules present great potential in the design of the new immunity balancerformulations according to their widespread structural diversity. Finally, we draw attention to the perspectives of modern trends in mushroom nutraceutical and cosmeceutical formulations to strengthen and balance immunity.
- Published
- 2023
- Full Text
- View/download PDF
30. Компаративни прглед здравствених ефеката комерцијално узгајане гљиве Agaricus bisporus и самоникле врсте гливе Ganoderma resinaceum
- Author
-
Kozarski, Maja S., Klaus, Anita, Vunduk, Jovana, Jakovljević, Dragica, Jadranin, Milka, and Nikšić, Miomir
- Subjects
cytotoxicity ,hot water extracts ,lipid peroxidation ,enzyme inhibition - Abstract
The health promoting effects of hot water extracts obtained from fruiting bodies of the commercially cultivated mushroom Agaricus bisporus (AbHW) and the wild-growing mushroom Ganoderma resinaceum (GrHW) originating from northern Serbia are presented in this research. These abilities were compared in vitro by the prevention of lipid peroxidation (LPx) in a linoleic acid model system, inhibition of the angiotension converting I enzyme (ACE) that could help in the maintenance of a normal blood pressure level and strengthening the ability of the central cholinergic neuron by inhibiting the activity of acetylcholinesterase (AChE). Cytotoxic activities were observed towards selected human malignant (HeLa and K562) cell lines and normal- -human peripheral blood mononuclear cells (PBMC). GrHW contains higher phenolics (5.9 g (100 g)-1), inhibition of LPx (EC50 = 1.07 mg mL-1), ACE (IC50 = 0.54 mg mL-1) and AChE (IC50 = 0.37 mg mL-1), and exhibited a significant selectivity in the antitumour action against HeLa (IC50 = 0.14 mg mL-1) and K562 (IC50 = 0.11 mg mL-1) cells. AbHW contained higher total protein (6.4 g (100 g)-1), carbohydrate (75.4 g (100 g)-1) and β-glucan (55.1 g (100 g)-1) contents and induced significant proliferation of healthy PBMC from 152–116 % in the concentration range of 0.047–0.187 mg mL-1. The difference in the biological activity of the extracts provides guidance on their use as functional food. У овоме раду поређен је здраствени ефекaт врелих водених екстраката добијених из плодоносних тела комерцијално узгајане јестиве гљиве Agaricus bisporus (AbHW) и самоникле врсте гљиве Ganoderma resinaceum (GrHW) из региона северне Србије. Здраствени ефекат је поређен in vitro превенцијом липидне пероксидације (LPx) у модел систему линолеинске киселине, инхибицијом ангиотензин конвертујућег ензима (ACE) који има улогу у одржавању нормалног нивоа крвног притиска и јачањем способности централних холинергичких неурона инхибицијом активности ацетилхолинестеразе (AChE). Цитотоксична активност је праћена на хуманим ћелијама тумора грлића материц (HeLa) и ћелијама хроничне мијелоидне леукемије (K562), као и на здравим мононуклеарним ћелијама периферног крвотока (PBMC). GrHW је показао већи садржај фенолних компоненти (5,9 g (100 g)-1), већу способност инхибиције LPx (EC50 = 1,07 mg mL-1), ACE (IC50 = 0,54 mg mL-1) и AChE (IC50 = 0,37 mg mL-1); показао је већу селективност у антитуморском дејству према HeLa (IC50 = 0,14 mg mL-1) и K562 (IC50 = 0,11 mg mL-1) ћелијама. AbHW је показао већи укупни садржај протеина (6,4 g (100 g)-1), угљених хидрата (75,4 g (100 g)-1) и β-глукана (55,1 g (100 g)-1) и значајно je стимулисао пролиферацију PBMC ћелија од 152–116 % у распону концентрација од 0,046–0,187 mg mL-1. Разлика у биолошкој активности екстраката даје смернице у њиховој примени као функционалнe хранe.
- Published
- 2020
31. Health impact of the commercially cultivated mushroom Agaricus bisporus and the wild-growing mushroom Ganoderma resinaceum - A comparative overview
- Author
-
Kozarski, Maja and Kozarski, Maja
- Abstract
The health promoting effects of hot water extracts obtained from fruiting bodies of the commercially cultivated mushroom Agaricus bisporus (AbHW) and the wild-growing mushroom Ganoderma resinaceum (GrHW) originating from northern Serbia are presented in this research. These abilities were compared in vitro by the prevention of lipid peroxidation (LPx) in a linoleic acid model system, inhibition of the angiotension converting I enzyme (ACE) that could help in the maintenance of a normal blood pressure level and strengthening the ability of the central cholinergic neuron by inhibiting the activity of acetylcholinesterase (AChE). Cytotoxic activities were observed towards selected human malignant (HeLa and K562) cell lines and normal-human peripheral blood mononuclear cells (PBMC). GrHW contains higher phenolics (5.9 g (100 g)(-1)), inhibition of LPx (EC50 = 1.07 mg mL(-1)), ACE (IC50 = 0.54 mg mL(-1)) and AChE (IC50 = 0.37 mg mL(-1)), and exhibited a significant selectivity in the antitumour action against HeLa (IC50 = 0.14 mg mL(-1)) and K562 (IC50 = 0.11 mg mL(-1)) cells. AbHW contained higher total protein (6.4 g (100 g)(-1)), carbohydrate (75.4 g (100 g)(-1)) and beta-glucan (55.1 g (100 g)(-1)) contents and induced significant proliferation of healthy PBMC from 152-116 % in the concentration range of 0.047-0.187 mg mL(-1). The difference in the biological activity of the extracts provides guidance on their use as functional food.
- Published
- 2020
32. The influence of mushroom Coriolus versicolor and hazelnuts enrichment on antioxidant activities and bioactive content of dark chocolate
- Author
-
Kozarski, Maja and Kozarski, Maja
- Abstract
Oxidative stress is among the main culprits for the progression of chronic diseases such as cardiovascular diseases, diabetes, and neurodegenerative disorders. It is well known that dark chocolate possesses polyphenols as major constituents which dietary consumption has been associated to health beneficial effects. Consequently, this study aimed to analyze dark chocolate (DC) and dark chocolate enriched with medicinal mushroom Coriolus versicolor (DCC) and hazelnuts (DCH) regarding antioxidant potential and total polyphenol (TPC), flavonoid (TFC), flavan-3-ol (FLA) and proanthocyanidin (PCA) content. DPPH•, CUPRAC and ABTS•+ assays were applied for measuring antioxidant capacity. The average of all antioxidant tests for each product was used for calculating the antioxidant potency composite index (ACI). The mean values of all antioxidant assays indicated that all chocolate products contained potent antioxidants. The addition of hazelnuts to dark chocolate significantly affected its total flavonoid content. Regression analysis among results obtained with antioxidant assays revealed that dark chocolate and dark chocolate products may act in mixed mode, by direct reduction via electron transfer or by radical quenching via H atom transfer. Flavan-3ols had the most significant impact on the ability of the analyzed samples to reduce metals, while proanthocyanidins primarily acted as radical scavengers. The obtained results provided additional information regarding the value-added dark chocolates enriched with bioactive compounds of medicinal mushroom and plant origin., Oksidativni stres je odgovoran za napredovanje hroničnih oboljenja kao što su kardiovaskularne bolesti, dijabetes i neurodegenerativni poremećaji. Poznato je da crna čokolada sadrži polifenole čiji unos ostvaruje pozitivan uticaj na zdravlje. Shodno tome, cilj ovog istraživanja bio je da se analizira antioksidativni potencijal i ukupni sadržaj polifenola (TPC), flavonoida (TFC), flavan-3-ola (FLA) i proantocijanidina (PCA) crne čokolade (DC) i crne čokolade sa dodatkom gljive Coriolus versicolor (DCC) i lešnika (DCH). Antioksidativni potencijal poređen je primenom DPPH•, CUPRAC i ABTS•+ testova za merenje antioksidativnih sposobnosti in vitro. Srednja vrednost svih antioksidativnih testova za svaki analizirani proizvod korišćena je za izračunavanje antioksidativnog kompozitnog indeksa (ACI). Rezultati istraživanja su pokazali da DC, DCC i DCH imaju izraženi antioksidativni potencijal. Dodavanje lešnika tamnoj čokoladi značajno je uticalo na njen ukupni sadržaj flavonoida. Regresiona analiza rezultata dobijenih antioksidativnim testovima je potvrdila da DC, DCC i DCH ispoljavaju antioksidativni potencijal različitim reakcionim mehanizmima tj. direktnom redukcijom prenošenjem elektrona i hvatanjem slobodnih radikala prenošenjem vodonikovih atoma. Potvrđeno je da flavan-3-oli imaju značajan uticaj na sposobnost analiziranih uzoraka da redukuju metale, dok proantocijanidini uglavnom deluju kao hvatači slobodnih radikala. Dobijeni rezultati ovog istraživanja pružaju dodatne informacije o biološkom potencijalu crne čokolade sa dodatkom bioaktivnih komponenata biljnog porekla i lekovitih gljiva.
- Published
- 2020
33. The influence of grape pomace substrate on quality characterization of Pleurotus ostreatus —Total quality index approach
- Author
-
Doroški, Ana, primary, Klaus, Anita, additional, Kozarski, Maja, additional, Cvetković, Stefana, additional, Nikolić, Biljana, additional, Jakovljević, Dragica, additional, Tomasevic, Igor, additional, Vunduk, Jovana, additional, Lazić, Vesna, additional, and Djekic, Ilija, additional
- Published
- 2020
- Full Text
- View/download PDF
34. Ganoderma lucidum as a cosmeceutical: antiradical potential and inhibitory effect on hyperpigmentation and skin extracellular matrix degradation enzymes
- Author
-
Kozarski, Maja and Kozarski, Maja
- Abstract
Three different polysaccharide extracts of a wild European source of the mushroom Ganoderma lucidum were screened for their free radical-blocking potential, which could strengthen the skin's barrier function, and provide a skin-lightening effect via potential inhibition of tyrosinase. The anti-collagenase and anti-elastase activities, which can help to restore skin elasticity and tensile strength, were also evaluated for the three extracts. Carbohydrates were the most abundant components of the extracts, followed by smaller quantities of proteins, phenols and flavonoids. The glucan fraction represented between 48% and 61% of carbohydrate content in all three extracts. None of the analyzed extracts showed overt toxicity to spontaneously-transformed immortal human keratinocytes (HaCaT) at concentrations up to 2 mg/mL, and displayed superior scavenging ability on 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals. The hot water crude polysaccharide extract (HWCP) and partially purified fraction (HWPP) were found to be effective inhibitors of lipid peroxidation (LPx), with an almost two-fold increased inhibition of LPx compared with ascorbic acid (EC50 = 1.65 +/- 0.08 mg/mL), a common additive in cosmeceutical formulations and used at mg levels. Among the investigated extracts, HWCP showed the strongest inhibition potential on tyrosinase and skin extracellular matrix (ECM) degradation enzymes. These diverse functionalities indicate that G. lucidum may represent a promising source of natural cosmeceutical ingredients.
- Published
- 2019
35. Antioxidative potential of the oyster mushroom (Pleurotus ostreatus) cultivated on grape pomace and straw substrate
- Author
-
Doroški, Ana, Kozarski, Maja, Klaus, Anita, Nikšić, Miomir, Vunduk, Jovana, and Djekić, Ilija
- Published
- 2018
- Full Text
- View/download PDF
36. The influence of grape pomace substrate on quality characterization of Pleurotus ostreatus—Total quality index approach.
- Author
-
Doroški, Ana, Klaus, Anita, Kozarski, Maja, Cvetković, Stefana, Nikolić, Biljana, Jakovljević, Dragica, Tomasevic, Igor, Vunduk, Jovana, Lazić, Vesna, and Djekic, Ilija
- Subjects
CULTIVATED mushroom ,WHEAT straw ,PLEUROTUS ostreatus ,TOTAL quality management ,CHEMICAL composition of plants ,GRAPES ,HAZARDOUS wastes - Abstract
Total quality index approach was used to reflect the quality of Pleurotus ostreatus mushroom, cultivated on grape pomace (P) as food waste substrate and wheat straw (S), in four different combined ratios: 100P; 80P20S; 50P50S; and 20P80S, with 100S, serving as control substrate. This approach involved two directions of mushroom analysis. The whole mushroom fruiting bodies analysis introduced a single total quality index (TQIM) based on 11 quality parameters. The mushroom water extracts analysis was presented by another single total quality index (TQIE), with seven quality parameters. This mathematical model revealed that the mushroom extracts have a better overall quality score with higher pomace content in substrate mixture, compared to the whole mushroom quality changes throughout the observed shelf life period. Future research may pay attention to the phytotoxicity of grape pomace as a food waste, as well as the influence of grape pomace on glucan content, as a health‐beneficial compounds. Practical applications: From the sustainable part of view, advantage of this type of production is its belonging to the sustainable food waste management strategy. The main importance of food waste use is their exploitation through bioconversion process into the new value‐added products, generating near zero waste. Also, the type and chemical composition of plant waste used as mushroom substrate affects the nutritional profile and quantity of the produced mushroom. Therefore, the application of this principle of production benefits winemakers on the one hand, eliminating the generation of toxic waste and protecting the environment. From a quality point of view, approach outlined in this research shows practical application of using quality index as a tool for overall quality evaluation. Finally, combining food waste for producing mushrooms evaluated through a quality index perspective paves the way for increasing mushroom in the mushroom values chain. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
37. Total quality index of Agaricus bisporus mushrooms packed in modified atmosphere
- Author
-
Đekić, Ilija, Vunduk, Jovana, Tomašević, Igor, Kozarski, Maja, Petrović, Predrag, Nikšić, Miomir, and Klaus, Anita
- Subjects
modified atmosphere packaging ,total quality index ,Agaricus bisporus ,quality characteristics - Abstract
BACKGROUND: The aim of this study was to develop a total quality index and examine the effects of modified atmosphere packaging (MAP) on the quality of Agaricus bisporus mushrooms stored for 22 days at 4 ∘C. Mushrooms were packaged under three MAPs: high nitrogen packaging (HNP), low carbon dioxide packaging (LCP) and low oxygen packaging (LOP). Passive MAP with air inside initially was used as the atmosphere treatment (AIR). RESULTS: This research revealed two phases in quality deterioration of A. bisporus mushrooms. During the first week, most of the quality parameters were not statistically different. Thereafter, odor intensities were stronger for all four types of packaging. Color difference and browning index values showed significantly lower color changes for AIR and LOP compared with HNP and LCP mushrooms. CONCLUSION: The best total quality index was calculated for LOP, followed by LCP and AIR. The findings of this study are useful with respect to examining two-component MAPs, separating the limiting factors (O2 and CO2) and evaluating quality deterioration effects and the total quality index of A. bisporus mushrooms. © 2016 Society of Chemical Industry
- Published
- 2017
38. Application of quality function deployment on shelf-life analysis of Agaricus bisporus Portobello
- Author
-
Djekic, Ilija, primary, Vunduk, Jovana, additional, Tomašević, Igor, additional, Kozarski, Maja, additional, Petrovic, Predrag, additional, Niksic, Miomir, additional, Pudja, Predrag, additional, and Klaus, Anita, additional
- Published
- 2017
- Full Text
- View/download PDF
39. Total quality index of Agaricus bisporus mushrooms packed in modified atmosphere
- Author
-
Djekic, Ilija, primary, Vunduk, Jovana, additional, Tomašević, Igor, additional, Kozarski, Maja, additional, Petrovic, Predrag, additional, Niksic, Miomir, additional, Pudja, Predrag, additional, and Klaus, Anita, additional
- Published
- 2016
- Full Text
- View/download PDF
40. Fenological Characteristics of the Wines of Merlot Variety (Vitis Vinifera L.) and Clones Selected in Serbia
- Author
-
Vujović, Dragan, Popović-Djordjević, Jelena, Kosović, Aleksandra, and Kozarski, Maja
- Subjects
ashes ,Merlot ,vino ,pepeo ,polifenoli ,wine ,total acidity ,polyphenols ,ukupne kiseline - Abstract
The study of the wine quality of Merlot variety standard and clones 022, 025 and 029, obtained in the third phase of clonal selection of Merlot variety was done in the period 2009-2012. The following components were measured: pH value of wines, total acidity, aldehydes, esters, total polyphenols and ashes. The largest content of esters and aldehydes was found in the wine of clone 025 and was significantly different compared to the wines of standard and other clones. The content of polyphenols, total acidity and pH values between the wines of standard and clones were not significantly different. Ash content in the tested wines ranged from 2.623 g/l to 2.748 g/l, indicating their authenticity. The studied wines are of good quality. U periodu 2009.-2012. godine izvršeno je ispitivanje kvaliteta vina standarda sorte Merlot i klonova 022, 025 i 029, dobijenih klonskom selekcijom trećeg vegetativnog potomstva sorte Merlot. Određeni su pH vrednost vina, ukupne kiseline, aldehidi, estri, ukupni polifenoli i pepeo. Sadržaj estara i aldehida je najveći u vinu klona 025 i statistički se značajno razlikuje u poređenju sa vinom standarda i ostalih klonova. Sadržaj polifenola, ukupnih kiselina i pH vrednosti između vina standarda i klonova se statistički značajno ne razlikuju. Sadržaj pepela u ispitivanim vinima je od 2.623 g/l do 2.748 g/l, što ukazuje na njihovu autentičnost. Ispitivana vina su dobrog kvaliteta.
- Published
- 2015
41. Antitumor activities of some macrofungi extracts
- Author
-
Zizak, Z., Klaus, Anita, Kozarski, Maja, Vunduk, Jovana, Nikšić, Miomir, and Juranić, Zorica
- Published
- 2014
42. Antibacterial and antifungal potential of wild basidiomycete mushroom Ganoderma applanatum
- Author
-
Klaus, Anita, primary, Kozarski, Maja, additional, Vunduk, Jovana, additional, Petrovic, Predrag, additional, and Niksic, Miomir, additional
- Published
- 2016
- Full Text
- View/download PDF
43. Addition of Zeolites to Improve the Functional Characteristics of the Hen of the Wood or Maitake Medicinal Mushroom, Grifola frondosa (Agaricomycetes)
- Author
-
Vunduk, Jovana, primary, Klaus, Anita, additional, Kozarski, Maja, additional, Dordevic, Radovan, additional, Miladinovic, Zoran, additional, Jovanovic, Ljubinko, additional, Niksic, Miomir, additional, and Van Griensven, Leonardus J.L.D., additional
- Published
- 2016
- Full Text
- View/download PDF
44. Antioxidants of Edible Mushrooms
- Author
-
Kozarski, Maja and Kozarski, Maja
- Abstract
Oxidative stress caused by an imbalanced metabolism and an excess of reactive oxygen species (ROS) lead to a range of health disorders in humans. Our endogenous antioxidant defense mechanisms and our dietary intake of antioxidants potentially regulate our oxidative homeostasis. Numerous synthetic antioxidants can effectively improve defense mechanisms, but because of their adverse toxic effects under certain conditions, preference is given to natural compounds. Consequently, the requirements for natural, alternative sources of antioxidant foods identified in edible mushrooms, as well as the mechanistic action involved in their antioxidant properties, have increased rapidly. Chemical composition and antioxidant potential of mushrooms have been intensively studied. Edible mushrooms might be used directly in enhancement of antioxidant defenses through dietary supplementation to reduce the level of oxidative stress. Wild or cultivated, they have been related to significant antioxidant properties due to their bioactive compounds, such as polyphenols, polysaccharides, vitamins, carotenoids and minerals. Antioxidant and health benefits, observed in edible mushrooms, seem an additional reason for their traditional use as a popular delicacy food. This review discusses the consumption of edible mushrooms as a powerful instrument in maintaining health, longevity and life quality.
- Published
- 2015
45. Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms
- Author
-
Kozarski, Maja and Kozarski, Maja
- Abstract
The methanolic extract of the wild edible mushroom Cantharellus cibarius Fr. (chanterelle) was analyzed for in vitro antioxidative, cytotoxic, antihypertensive and antibacterial activities. Various primary and secondary metabolites were found. Phenols were the major antioxidant components found in the extract (49.8 mg g(-1)), followed by flavonoids, whose content was approximately 86% of the total phenol content. Antioxidant activity, measured by four different methods, was high for inhibition of lipid peroxidation (EC50 = 1.21 mg mL(-1)) and chelating ability (EC50 = 0.64 mg mL(-1)). The antioxidant activity of the C. cibarius methanol extract was achieved through chelating iron compared to hydrogen atom and/or electron transfer. The extract showed good selectivity in cytotoxicity on human cervix adenocarcinoma HeLa, breast carcinoma MDA-MB-453 and human myelogenous leukemia K562, compared to normal control human fetal lung fibroblasts MRC-5 and human lung bronchial epithelial cells BEAS-2B. The extract had inhibitory activity against angiotensin converting I enzyme (ACE) (IC50 = 0.063 mg mL(-1)). The extract revealed selective antimicrobial activity against Gram-positive bacteria with the highest potential against E. faecalis. The medicinal and health benefits, observed in wild C. cibarius mushroom, seem an additional reason for its traditional use as a popular delicacy food.
- Published
- 2015
46. Antioxidants of Edible Mushrooms
- Author
-
Kozarski, Maja, primary, Klaus, Anita, additional, Jakovljevic, Dragica, additional, Todorovic, Nina, additional, Vunduk, Jovana, additional, Petrović, Predrag, additional, Niksic, Miomir, additional, Vrvic, Miroslav, additional, and van Griensven, Leo, additional
- Published
- 2015
- Full Text
- View/download PDF
47. Antioxidant and antimicrobial potentials of champignon mushroom
- Author
-
Kozarski, Maja, Klaus, Anita, Nikšić, Miomir, Jakovljević, Dragica, and Vrvić, Miroslav M.
- Subjects
Antimicrobial ,Agaricus bisporus ,Antioxidant ,Polysaccharide extract - Abstract
Fruiting bodies of some wild and cultivatable mushrooms contain medicinal compounds which are being used in traditional medicines and cosmetics. Champignon mushroom (Agaricus bisporus) is the most widely cultivated species of edible mushroom worldwide. This paper focuses on antioxidant and antimicrobial importance of A bisporus. Water-soluble polysaccharide-enriched fraction was isolated from the dry carpophores of Agaricus bisporus. Antioxidant activities were investigated using in vitro assay systems: 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging and chelating ability on ferrous ions. Antimicrobial activity was tested against Gram positive and Gram negative bacteria in vitro by disk diffusion method in order to determine the zones of inhibition. At concentrations of 0.1-10 mg/ml, the scavenging abilities of A bisporus ranged between 12.3-75.5 %. The radical scavenging ability of the positive controls-tocopherol and ascorbic acid, at the concentrations of 0.1-20 mg/ml, were between 79.9-80.8 and 80.6-91.1 %, respectively. Polysaccharide extract from A bisporus showed steadily increasing chelating ability as concentrations increased to 88.2 % at 20 mg/ml. The chelating ability of the citric acid was between 7.2-10.7 %, at the concentrations of 0.1-20 mg/ml. The study of antimicrobial potential of polysaccharide extract showed more potent activity against Gram-positive Enterococcus faecalis ATCC 49532 (26.7 ± 0.2 mm), Bacillus cereus 10876 (27.5 ± 0.4 mm), Geobacillus stearothermophylus ATCC 7953 (22.8 ± 0.3 mm) than Gram-negative bacteria Pseudomonas aeruginosa ATCC 35032 (10.4 ± 0.6 mm), Proteus hauseri ATCC 13315 (12.1 ± 0.1 mm) Escherichia coli (0157:H7) 35150 (12.7 ± 0.4 mm) with exception of Klebsiella pneumoniae ATCC 27736 (22.3 ± 0.2 mm).
- Published
- 2012
48. Hemijska karakterizacija, antioksidativna i antimikrobna svojstva polisaharidnih ekstrakata odabranih vrsta gljiva
- Author
-
Kozarski, Maja S., Vrvić, Miroslav M., Jakovljević, Dragica M., Tešević, Vele, Nikšić, Miomir, and Todorović, Nina
- Subjects
chelating ability ,antimicrobial properties ,polysaccharides ,phenols ,moć redukcije ,DPPH scavenging activity ,mikrobicidno dejstvo ,reducing power ,food and medicine ,sposobnost hvatanja slobodnih DPPH radikala ,polisaharidi ,gljive ,inhibition of lipid peroxidation ,microbiostatic ,mikrobiostatsko dejstvo ,hrana i lekovi ,mushrooms ,proteins ,antioxidant properties ,fenoli ,antioksidativnasvojstva ,antimikrobna svojstva ,proteini ,sposobnost heliranja ,microbicidal ,inhibicija peroksidacije lipida - Abstract
Polisaharidi gljiva, biljaka i mikroorganizama privlače veliku pažnju zbog svojihinteresantnih bioloških svojstava kao što su: antitumorno, antivirusno, antioksidativno,antikomplementarno, antikoagulaciono, hipolipidemijsko dejstvo, kao iimunomodulatorska i imunostimulativna aktivnost, što ih sve čini pogodnim za primenuu mnogim oblastima, među kojima su i prehrambena industrija, kozmetika,biomedicina, poljoprivreda, zaštita životne sredine i otpadnih voda.Oksidativna oštećenja prirodnih i industrijski pripremljenih namirnica predstavljajuveliki ekonomski problem u prehrambenoj industriji, jer direktno utiču na karakteristikekvaliteta kao što su ukus, boja, tekstura i nutritivne vrednosti. Pored toga, može doći donastanka potencijalno toksičnih proizvoda. Reaktivne vrste kiseonika učestvuju upatofiziologiji različitih kliničkih poremećaja, kao što su: ishemija, reperfuzionepovrede, infarkt miokarda, reumatoidni artritis, neurodegenerativni poremećaji,ateroskleroza, hipertenzija, hemoragički šok i dijabetes.Mikrobiološko kvarenje hrane i trovanje hranom su jedan od najvažnijih problema sakojima se suočava prehrambena industrija. Alimentarne intoksikacije su danas globalniproblem. Porast rezistentnosti patogena na poznate lekove stvara potrebu za razvojemnovih antimikrobnih agenasa.U ovom radu su proučavana antioksidativna i antimikrobna svojstva polisaharidnihekstrakata odabranih vrsta gljiva iz klase Basidiomycetes: Agaricus bisporus, Agaricusbrasiliensis, Ganoderma applanatum, Ganoderma lucidum, Lentinula edodes, Phellinuslinteus i Trametes versicolor.Na osnovu dobijenih rezultata može se zaključiti da polisaharidni ekstrakti, dobijeninakon ekstrakcije vrelom vodom, alkoholne precipitacije i dijalize, predstavljajusmešu/komplekse polisaharida, proteina i fenola... Polysaccharides from mushrooms, plants and microorganisms have receivedconsiderable attention due to their biological activities, such as antitumor, antiviral,antioxidation, anticomplementary, anticoagulant, hypolipidemic, immunostimulant andimmunological activities, which made them possible to be used in many fields includingfood, cosmetics, biomedicine, agriculture, environmental protection and wastewatermanagement.Oxidative deterioration in natural and processed foods is a large economic concern inthe food industry because it affects many quality characteristics such as flavor, color,texture, and the nutritive value of foods. In addition, its products are potentially toxic.Also, reactive oxygen species have been implicated in the pathophysiology of variousclinical disorders, including ischemia, reperfusion injury, myocardial infarction,rheumatoid arthritis, neurodegenerative processes, atherosclerosis, acute hypertension,hemorrhagic shock and diabetes mellitus.Food spoilage and food poisoning are among the most important issues facing thefood industry. Foodborne illness is a global problem. Development of novelantibacterial compounds for resistant organisms is becoming critically important.The present study was focused on the researching of possible antioxidant andantimicrobial properties of polysaccharide extracts from the selected Basidiomycetesmushroom species: Agaricus bisporus, Agaricus brasiliensis, Ganoderma applanatum,Ganoderma lucidum, Lentinula edodes, Phellinus linteus and Trametes versicolor.Based on the analysis, the extracts contained a mixture/complex of polysaccharides,proteins and polyphenols, which were still present in the extracts after hot watertreatment, ethanol precipitation and dialysis...
- Published
- 2012
49. Biological potential of extracts of the wild edible Basidiomycete mushroom Grifola frondosa
- Author
-
Klaus, Anita, primary, Kozarski, Maja, additional, Vunduk, Jovana, additional, Todorovic, Nina, additional, Jakovljevic, Dragica, additional, Zizak, Zeljko, additional, Pavlovic, Vladimir, additional, Levic, Steva, additional, Niksic, Miomir, additional, and Van Griensven, Leo J.L.D., additional
- Published
- 2015
- Full Text
- View/download PDF
50. Nutraceutical properties of the methanolic extract of edible mushroom Cantharellus cibarius (Fries): primary mechanisms
- Author
-
Kozarski, Maja, primary, Klaus, Anita, additional, Vunduk, Jovana, additional, Zizak, Zeljko, additional, Niksic, Miomir, additional, Jakovljevic, Dragica, additional, Vrvic, Miroslav M., additional, and Van Griensven, Leo J. L. D., additional
- Published
- 2015
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.