1. A p53-dependent translational program directs tissue-selective phenotypes in a model of ribosomopathies
- Author
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Tiu, Gerald C, Kerr, Craig H, Forester, Craig M, Krishnarao, Pallavi S, Rosenblatt, Hannah D, Raj, Nitin, Lantz, Travis C, Zhulyn, Olena, Bowen, Margot E, Shokat, Leila, Attardi, Laura D, Ruggero, Davide, and Barna, Maria
- Subjects
Biochemistry and Cell Biology ,Biological Sciences ,Biotechnology ,Stem Cell Research - Nonembryonic - Non-Human ,Genetics ,Stem Cell Research ,Rare Diseases ,Generic health relevance ,Adaptor Proteins ,Signal Transducing ,Animals ,Body Patterning ,Cell Cycle Proteins ,Extremities ,Gene Expression Regulation ,Developmental ,Haploinsufficiency ,Mice ,Mice ,Knockout ,Phenotype ,Protein Biosynthesis ,Protein Processing ,Post-Translational ,Ribosomal Proteins ,Ribosomes ,Tumor Suppressor Protein p53 ,4E-BP1 ,limb development ,nucleolar stress ,p53 ,ribosomal protein haploinsufficiency ,ribosome profiling ,ribosomopathy ,translational control ,Medical and Health Sciences ,Developmental Biology ,Biochemistry and cell biology - Abstract
In ribosomopathies, perturbed expression of ribosome components leads to tissue-specific phenotypes. What accounts for such tissue-selective manifestations as a result of mutations in the ribosome, a ubiquitous cellular machine, has remained a mystery. Combining mouse genetics and in vivo ribosome profiling, we observe limb-patterning phenotypes in ribosomal protein (RP) haploinsufficient embryos, and we uncover selective translational changes of transcripts that controlling limb development. Surprisingly, both loss of p53, which is activated by RP haploinsufficiency, and augmented protein synthesis rescue these phenotypes. These findings are explained by the finding that p53 functions as a master regulator of protein synthesis, at least in part, through transcriptional activation of 4E-BP1. 4E-BP1, a key translational regulator, in turn, facilitates selective changes in the translatome downstream of p53, and this thereby explains how RP haploinsufficiency may elicit specificity to gene expression. These results provide an integrative model to help understand how in vivo tissue-specific phenotypes emerge in ribosomopathies.
- Published
- 2021