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5. State of the processes of free radical oxidation in acute liver damage

Author

Kantyukov, S. A., Krivokhizhina, L. V., and Farkhutdinov, R. R.

Subjects
ГРНТИ 76.29, свободно-радикальное окисление, liver damage, хемилюминесценция, УДК 616.36-002, поражение печени, chemiluminescence, free radical oxidation
Abstract

В данном исследовании изучено состояние процессов свободно-радикального окисления (СРО) в крови и гомогенате печени методом регистрации железоиндуцированной хемилюминесценции и определением продуктов перекисного окисления липидов (ПОЛ) при остром экспериментальном токсическом поражении печени четыреххлористым углеродом. Показано, что в первые часы острого отравления возрастает интенсивность хемилюминесценции и накапливаются продукты ПОЛ в ткани печени и крови, обусловленное снижением общей антиокислительности, появлением свободных радикалов, увеличением количества гидроперекисей липидов. Снижение показателей хемилюминесценции в гомогенате печени к 2-3-м суткам интоксикации относительно первых часов обусловлено возрастанием скорости окисления, повреждением клеток печени, но не усилением мощности общей антиокислительной системы. Характер изменений СРО в крови и печени в динамике ее острого повреждения различен. In the present research we studied the state of the processes of free radical oxidation in blood and homogenate of liver by means of the method of ironinduced chemiluminescence registration and by determining the products of lipid peroxidation (LPO) in acute experimental toxic damage of the liver by carbon tetrachloride. We showed that during the first hours of acute intoxication, the intensity of chemiluminescence increases, and the products of LPO accumulate in liver tissue and in blood, which is caused by the decrease of general antioxidation, by the appearance of free radicals, by the rise of the amount of lipid hydroperoxides. The reduction of chemiluminescence indices in liver homogenate by the end of 2-3 days of intoxication relative to the first hours is caused by the increase of oxidation speed, by the damage of liver cells, but not by the increase of the strength of the whole antioxidant system. The character of changes of free radical oxidation in blood and liver differs depending on the dynamic of acute liver damage. Кантюков С.А., кандидат медицинских наук, доцент кафедры биохимии, Челябинская государственная медицинская академия (Челябинск). Kantyukov S.A., the candidate of medical sciences, the senior lecturer, the senior lecturer of chair of biochemistry of the Chelyabinsk state medical academy (Chelyabinsk). Кривохижина Л.В., доктор медицинских наук, профессор, заведующая кафедрой патологической физиологии, Челябинская государственная медицинская академия (Челябинск); e-mail: LVK.777@mail.ru. Krivohizhina L.V., the doctor of medical sciences, the professor, managing chair of pathological physiology of the Chelyabinsk state medical academy (Chelyabinsk); e-mail: LVK.777@mail.ru. Фархутдинов P.P., доктор медицинских наук, профессор, Башкирский государственный медицинский университет (Уфа). Farkhutdinov R.R., the doctor of medical sciences, the professor Bashkir state medical university (Ufa).

Published
2011

6. State of processes of free-radical oxidation at the thermal trauma of different severity level

Author

Фархутдинов, P. P., Kantjukov, S. A., Krivokhizhina, L. V., and Farkhutdinov, R. R.

Subjects
ГРНТИ 76.29, thermal trauma, термическая травма, free-radical oxidation, свободно-радикальное окисление, УДК 617-001, УДК 577.121.7, хемилюминесценция, chemiluminescence
Abstract

В настоящем исследовании определена интенсивность свободно-радикального окисления при экспериментальной ожоговой травме средней и тяжелой степени тяжести. Свободно-радикальное окисление в крови исследовали методом железоиндуцированной хемилюминесценции. Показано, что термическая травма независимо от степени тяжести сопровождается активацией свободно-радикального окисления. Интенсивность свободно-радикального окисления зависит от тяжести ожоговой травмы. Для стадии ожоговой токсемии при ожогах 3—4 степени тяжести характерно снижение в крови гидроперекисей липидов и интенсивности окисления липидов. In the present research intensity of free-radical oxidation is defined at an experimental burning injury of middle and severe severity levels. The free-radical oxidation in blood was examined using the iron induced chemicoluminescence method. The authors demonstrate that thermal trauma irrespective of severity level is accompanied by the activation of freeradical oxidation. Its intensity depends on the severity of the burning injury. The burning toxemia of the 3-4 severity level burns is characterized by decrease of hydroperoxides of lipids and intensity of oxidation of lipids in blood. Кантюков С.А., кандидат медицинских наук, доцент, доцент кафедры биохимии Челябинской государственной медицинской академии (Челябинск);тел. 8-351-2627823. Kantjukov S.A., candidate of medical sciences, the senior lecturer biochemistry chair the Chelyabinsk State Medical Academy (Chelyabinsk); ph. 8-351-2627823. Кривохижина Л.В., доктор медицинских наук, профессор, заведующая кафедрой патофизиологии Челябинской государственной медицинской академии (Челябинск); e-mail:LVK.777@mail.ru Krivokhizhina L.V., doctor of medical sciences, professor, the manager. Pathophysiology chair Chely abinsk State Medical Academy (Chelyabinsk); e-mail: LVK.777@mail.ru. Фархутдинов P.P., доктор медицинских наук, профессор Башкирского государственного медицинского университета (Уфа). Farkhutdinov R.R., doctor of medical sciences, the professor the Bashkir State Medical University (Уфа).

Published
2010

7. Endotheliocyte functional status at terebrant affects of various genesis

Author

Osikov, M. V. and Krivokhizhina, L. V.

Subjects
ГРНТИ 76.29, endotheliocytes, dysfunction, эндотелиоциты, terebrant tissue damage, УДК 616.61-002, острое повреждение тканей, дисфункция
Abstract

В работе исследовано функциональное состояние эндотелиоцитов при острых повреждениях различного генеза по показателям продукции оксида азота (II) и адгезии к нему клеток крови. Установлено развитие дисфункции эндотелиоцитов при острой почечной недостаточности, острой печеночной недостаточности и септическом перитоните. Манифестация асептического перитонита не сопровождается изменением функционального состояния эндотелиоцитов. Суммарное содержание продуктов NO возрастает при острой почечной недостаточности, острой печеночной недостаточности и септическом перитоните. Адгезивная способность эндотелиоцитов изменяется при острой печеночной недостаточности и септическом перитоните по отношению к лейкоцитам. The work investigates the endotheliocyte functional status at terebrant affects of various genesis. The status is estimated by indicators of nitrogen oxide products (II) and blood cells conglutination to it. It is stated that acute renal failure, acute hepatic failure and septic peritonitis can induce the endothelial dysfunction. Demonstration of aseptic peritonitis is not accompanied by change of endotheliocyte functional status. Total content of nitrogen oxide products increases at acute renal failure, acute hepatic failure and septic peritonitis. At acute hepatic failure and septic peritonitis the adhesive capacity of endotheliocytes changes against leukocytes. Осиков Михаил Владимирович, кандидат медицинских наук, доцент кафедры патологической физиологии Челябинской государственной медицинской академии (г. Челябинск) (8-351-232-74-68). Osikov Mihail Vladimirovich, Candidate of medical sciences, Associate Professor of department of physiopathology of the Chelyabinsk State Medical Academy (Chelyabinsk) (8-351-232-74-68). Кривохижина Людмила Владимировна, заведующая кафедрой патологической физиологии Челябинской государственной медицинской академии (г. Челябинск) (8-351-232-74-68). Krivohizhina Ljudmila Vladimirovna, head of the department of physiopathology of the Chelyabinsk State Medical Academy (Chelyabinsk) (8-351-232-74-68).

Published
2009

8. [CERULOPLASMIN CORRECTION OF DYSLIPIDEMIA CAUSED BY CHRONIC PHYSICAL LOAD IN EXPERIMENT AT SUBMAXIMAL POWER.]

Author

Ermolaeva EN and Krivokhizhina LV

Subjects
Animals, Rats, Ceruloplasmin pharmacology, Dyslipidemias blood, Dyslipidemias drug therapy, Dyslipidemias etiology, Lipids blood, Physical Conditioning, Animal adverse effects
Abstract

Chronic submaximal physical load of aerobic-anaerobic charater leads to pro-atherogenic dyslipidemia, as manifested by increased levels of triglycerides, total cholesterol, and very-low-density lipoproteins. Ceruloplasmin (total dose 60 mg/kg body weight, triple intraperitoneal introduction on the 1st, 3rd, and 5th day of experiment) produced a partial correction of dyslipidemia, manifested by restoration of the normal levels of triglycerides, total cholesterol, and lipoproteins of very low, low, and high density. At the same time, ceruloplasmin administration did not influence the level of phospholipids.

Published
2016

9. [Influence of alpha-1-acid glycoprotein on blood rheology].

Author

Osikov MV, Krivokhizhina LV, and Makarov EV

Subjects
Animals, Cell Adhesion drug effects, Endothelium, Vascular, Male, Rats, Rats, Inbred Strains, Blood Coagulation drug effects, Blood Viscosity drug effects, Erythrocyte Aggregation drug effects, Orosomucoid pharmacology, Platelet Aggregation drug effects
Abstract

Hemorheologic effects of alpha-1-acid glycoprotein (AGP) were studied in experiment on 76 noninbred white male rats. AGP was administered i.p. to intact rats twice in a total dose of 300 mg/kg. The effects were evaluated 72 hours after the first injection. It was found that AGP accelerated platelet and erythrocyte aggregation, platelet-erythrocyte cooperation, enhanced adhesion of erythrocytes and agranulocytes to endothelium. On the contrary, adhesion of granulocytes and platelets to endothelium was diminished. Acceleration of platelet aggregation was mediated by stimulation of free radical processes in the cells. Thus, AGP produces anticoagulation action on blood theology.

Published
2007

11. [The action of the cells of hemopoietic organs in chick embryos on mouse CFUdc and CLFUdc].

Author

Krivokhizhina LV

Subjects
Animals, Cell Differentiation, Cell Division, Cells, Cultured cytology, Chick Embryo, Colony-Forming Units Assay, Diffusion Chambers, Culture, Liver cytology, Mice, Mice, Inbred CBA, Spleen cytology, Yolk Sac cytology, Bone Marrow Cells, Hematopoietic Stem Cells cytology
Abstract

The humoral influence of cells of hemopoietic organs of chicken embryos of different terms on the development of the colony and cluster formation of mononuclears of the bone marrow of mice was studied in joint cultivation in two-compartment cylindrical diffuse microchambers. The process of formation of colonies and clusters is inhibited by cells of the yolk sac on the 2nd-4th day of the development, by cells of the liver on the 8th-12th day, of the spleen on the 13th-18th day and of the bone marrow--on the 15th day. The yolk sac cells were found to have most considerable inhibiting influence on proliferation and differentiation of cells on the 2nd day of the development of chicken embryo. The yolk sac cells on the 6th day stimulate the formation of colonies and clusters. The yolk sac, beginning from the 4th day of the development, and the liver release humoral factors promoting the formation of erythroid colonies. The erythroid colonies are formed but when cultivated on the vascular membrane of the chicken embryo; the erythroid colonies are not formed when cultivated in the abdominal cavity of mice. Local erythropoietinoid factors are not synthetized by the spleen and bone marrow cells. A supposition is put forward that a combination of the local inhibiting and erythropoietic effects promotes the erythroid differentiation of cells.

Published
1991

12. [Colony- and cluster-forming ability of mouse bone marrow depending on its functional state and cultivation conditions].

Author

Krivokhizhina LV

Subjects
Animals, Cells, Cultured, Chick Embryo, Mice, Bone Marrow Cells, Hematopoietic Stem Cells physiology
Abstract

The dependence of colony- and cluster-forming ability (CFA and CLFA) of bone marrow cells on its initial state and the conditions of cultivation was studied. Intact mice and mice with stimulated erythropoiesis were used in the experiment. The bone marrow was separated on a density gradient and cultivated in diffusion chambers (DC) on a plasma clot with addition of the tissue fluid of a chick embryo. Uni- and bicameral milliporous cylindrical microchambers were used. The DC were put into the abdominal cavity of mice first given a cyclophosphamide injection and on the vascular membrane of the chick embryo. The bicameral DC were employed in cocultivation of yolk-sac cells of a 4-week-old chick embryo and bone marrow mononuclears of both experimental groups. The CFA and CLFA of bone marrow mononuclears depend in respect of quantity and quality on the condition of its cultivation rather than on its initial state. It is concluded on the basis of tests for the inductive effects of yolk-sac cells on the CFA and CLFA that in study of cell inductors under conditions of DC and in cultivation in vivo one should take into account the initial state of the hematopoietic target cells, the sensitivity of cells to stimulation, the conditions of cultivation which allow the cells to emerge into the process of this or that differentiation even in the presence of an inductor.

Published
1991

13. [The effect of the humoral action of the cells from chick embryo hematopoietic organs on the colony-forming capacity of normal mouse bone-marrow cells and in activated erythropoiesis].

Author

Krivokhizhina LV

Subjects
Animals, Bone Marrow drug effects, Bone Marrow physiology, Cells, Cultured cytology, Cells, Cultured drug effects, Cells, Cultured physiology, Chick Embryo, Colony-Forming Units Assay, Diffusion Chambers, Culture, Erythropoiesis drug effects, Mice, Mice, Inbred CBA, Phenylhydrazines pharmacology, Stimulation, Chemical, Time Factors, Bone Marrow Cells, Erythropoiesis physiology, Hematopoietic Stem Cells physiology
Abstract

The cells of chick embryo hematopoietic organs were cultivated together with mouse bone marrow cells in bicameral diffusion chambers under normal conditions and in activated erythropoiesis. The yolk sac cells inhibited colony formation on the 2nd, 4th days of development, the hepatic cells--on the 8th, 12th days, the splenic cells--on the 13th, 17th days, and the bone marrow cells--on the 15th day. The cells of the yolk sac sharply intensify the formation of colonies by the marrow mononuclears, in particular, on the 6th developmental day in activated erythropoiesis. The yolk sac (on the 4th, 6th days of development), the liver, and the spleen produce humoral factors which facilitate the formation of erythroid colonies by the marrow cells of mice. The cells of a 4-day-old yolk sac inhibit the formation of granulocytic colonies, and the synthesis of inhibitors ceases in a 6-day-old yolk sac. It is suggested that the synthesis of local humoral inhibitors of the proliferation of colony-forming units controls the intensity of hematopoiesis in the organ and, by reducing proliferation, facilitates the emergency of cells into differentiation independently, according to the law or under the effect of local humoral poetins.

Published
1991

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