Search

Your search keyword '"Kropp, Christina"' showing total 13 results
Start Over Author "Kropp, Christina"
13 results on '"Kropp, Christina"'

5. High Density Bioprocessing of Human Pluripotent Stem Cells by Metabolic Control and in Silico Modeling

Author

Manstein, Felix, Ullmann, Kevin, Kropp, Christina, Halloin, Caroline, Triebert, Wiebke, Franke, Annika, Farr, Clara-Milena, Sahabian, Anais, Haase, Alexandra, Breitkreuz, Yannik, Peitz, Michael, Brüstle, Oliver, Kalies, Stefan, Martin, Ulrich, Olmer, Ruth, Zweigerdt, Robert, Manstein, Felix, Ullmann, Kevin, Kropp, Christina, Halloin, Caroline, Triebert, Wiebke, Franke, Annika, Farr, Clara-Milena, Sahabian, Anais, Haase, Alexandra, Breitkreuz, Yannik, Peitz, Michael, Brüstle, Oliver, Kalies, Stefan, Martin, Ulrich, Olmer, Ruth, and Zweigerdt, Robert

Abstract

To harness the full potential of human pluripotent stem cells (hPSCs) we combined instrumented stirred tank bioreactor (STBR) technology with the power of in silico process modeling to overcome substantial, hPSC-specific hurdles toward their mass production. Perfused suspension culture (3D) of matrix-free hPSC aggregates in STBRs was applied to identify and control process-limiting parameters including pH, dissolved oxygen, glucose and lactate levels, and the obviation of osmolality peaks provoked by high density culture. Media supplements promoted single cell-based process inoculation and hydrodynamic aggregate size control. Wet lab-derived process characteristics enabled predictive in silico modeling as a new rational for hPSC cultivation. Consequently, hPSC line-independent maintenance of exponential cell proliferation was achieved. The strategy yielded 70-fold cell expansion in 7 days achieving an unmatched density of 35 × 106 cells/mL equivalent to 5.25 billion hPSC in 150 mL scale while pluripotency, differentiation potential, and karyotype stability was maintained. In parallel, media requirements were reduced by 75% demonstrating the outstanding increase in efficiency. Minimal input to our in silico model accurately predicts all main process parameters; combined with calculation-controlled hPSC aggregation kinetics, linear process upscaling is also enabled and demonstrated for up to 500 mL scale in an independent bioreactor system. Thus, by merging applied stem cell research with recent knowhow from industrial cell fermentation, a new level of hPSC bioprocessing is revealed fueling their automated production for industrial and therapeutic applications.

Published
2021

6. High Density Bioprocessing of Human Pluripotent Stem Cells by Metabolic Control and in Silico Modeling

Author

Manstein, Felix, primary, Ullmann, Kevin, additional, Kropp, Christina, additional, Halloin, Caroline, additional, Triebert, Wiebke, additional, Franke, Annika, additional, Farr, Clara-Milena, additional, Sahabian, Anais, additional, Haase, Alexandra, additional, Breitkreuz, Yannik, additional, Peitz, Michael, additional, Brüstle, Oliver, additional, Kalies, Stefan, additional, Martin, Ulrich, additional, Olmer, Ruth, additional, and Zweigerdt, Robert, additional

Published
2021
Full Text
View/download PDF

10. Sensitivity of human pluripotent stem cells to insulin precipitation induced by peristaltic pump-based medium circulation: considerations on process development

Author

Massai, Diana, primary, Bolesani, Emiliano, additional, Diaz, Diana Robles, additional, Kropp, Christina, additional, Kempf, Henning, additional, Halloin, Caroline, additional, Martin, Ulrich, additional, Braniste, Tudor, additional, Isu, Giuseppe, additional, Harms, Vanessa, additional, Morbiducci, Umberto, additional, Dräger, Gerald, additional, and Zweigerdt, Robert, additional

Published
2017
Full Text
View/download PDF

11. Impact of Feeding Strategies on the Scalable Expansion of Human Pluripotent Stem Cells in Single-Use Stirred Tank Bioreactors

Author

Kropp, Christina, primary, Kempf, Henning, additional, Halloin, Caroline, additional, Robles-Diaz, Diana, additional, Franke, Annika, additional, Scheper, Thomas, additional, Kinast, Katharina, additional, Knorpp, Thomas, additional, Joos, Thomas O., additional, Haverich, Axel, additional, Martin, Ulrich, additional, Zweigerdt, Robert, additional, and Olmer, Ruth, additional

Published
2016
Full Text
View/download PDF

12. Controlling Expansion and Cardiomyogenic Differentiation of Human Pluripotent Stem Cells in Scalable Suspension Culture

Author

Kempf, Henning, primary, Olmer, Ruth, additional, Kropp, Christina, additional, Rückert, Michael, additional, Jara-Avaca, Monica, additional, Robles-Diaz, Diana, additional, Franke, Annika, additional, Elliott, David A., additional, Wojciechowski, Daniel, additional, Fischer, Martin, additional, Roa Lara, Angelica, additional, Kensah, George, additional, Gruh, Ina, additional, Haverich, Axel, additional, Martin, Ulrich, additional, and Zweigerdt, Robert, additional

Published
2014
Full Text
View/download PDF

13. Sensitivity of human pluripotent stem cells to insulin precipitation induced by peristaltic pump-based medium circulation: Considerations on process development

Author

Massai, Diana, Bolesani, Emiliano, Diaz, Diana R., Kropp, Christina, Kempf, Henning, Halloin, Caroline, Martin, Ulrich, Braniste, Tudor, Isu, Giuseppe, Harms, Vanessa, Morbiducci, Umberto, Dräger, Gerald, and Zweigerdt, Robert

Subjects
2. Zero hunger, human pluripotent stem cells, Dewey Decimal Classification::600 | Technik::610 | Medizin, Gesundheit, hPSCs
Abstract

Controlled large-scale production of human pluripotent stem cells (hPSCs) is indispensable for their envisioned clinical translation. Aiming at advanced process development in suspension culture, the sensitivity of hPSC media to continuous peristaltic pump-based circulation, a well-established technology extensively used in hydraulically-driven bioreactors, was investigated. Unexpectedly, conditioning of low protein media (i.e. E8 and TeSR-E8) in a peristaltic pump circuit induced severe viability loss of hPSCs cultured as aggregates in suspension. Optical, biochemical, and cytological analyses of the media revealed that the applied circulation mode resulted in the reduction of the growth hormone insulin by precipitation of micro-sized particles. Notably, in contrast to insulin depletion, individual withdrawal of other medium protein components (i.e. bFGF, TGFβ1 or transferrin) provoked minor reduction of hPSC viability, if any. Supplementation of the surfactant glycerol or the use of the insulin analogue Aspart did not overcome the issue of insulin precipitation. In contrast, the presence of bovine or human serum albumin (BSA or HSA, respectively) stabilized insulin rescuing its content, possibly by acting as molecular chaperone-like protein, ultimately supporting hPSC maintenance. This study highlights the potential and the requirement of media optimization for automated hPSC processing and has broad implications on media development and bioreactor-based technologies. © 2017 The Author(s).

Catalog

Books, media, physical & digital resources
See catalog results