Search

Your search keyword '"Kud, Joanna"' showing total 15 results
Start Over Author "Kud, Joanna"
15 results on '"Kud, Joanna"'

3. Belowground Chemical Interactions: An Insight Into Host-Specific Behavior of Globodera spp. Hatched in Root Exudates From Potato and Its Wild Relative, Solanum sisymbriifolium

Author

Kud, Joanna, Pillai, Syamkumar Sivasankara, Raber, Gabriel, Caplan, Allan, Kuhl, Joseph C., Xiao, Fangming, and Dandurand, Louise-Marie

Subjects
belowground defenses, Solanum sisymbriifolium, pest management, Globodera spp, fungi, food and beverages, potato, Plant culture, RNA-Seq, Plant Science, root exudates, Original Research, SB1-1110
Abstract

Understanding belowground chemical interactions between plant roots and plant-parasitic nematodes is immensely important for sustainable crop production and soilborne pest management. Due to metabolic diversity and ever-changing dynamics of root exudate composition, the impact of only certain molecules, such as nematode hatching factors, repellents, and attractants, has been examined in detail. Root exudates are a rich source of biologically active compounds, which plants use to shape their ecological interactions. However, the impact of these compounds on nematode parasitic behavior is poorly understood. In this study, we specifically address this knowledge gap in two cyst nematodes, Globodera pallida, a potato cyst nematode and the newly described species, Globodera ellingtonae. Globodera pallida is a devastating pest of potato (Solanum tuberosum) worldwide, whereas potato is a host for G. ellingtonae, but its pathogenicity remains to be determined. We compared the behavior of juveniles (J2s) hatched in response to root exudates from a susceptible potato cv. Desirée, a resistant potato cv. Innovator, and an immune trap crop Solanum sisymbriifolium (litchi tomato – a wild potato relative). Root secretions from S. sisymbriifolium greatly reduced the infection rate on a susceptible host for both Globodera spp. Juvenile motility was also significantly influenced in a host-dependent manner. However, reproduction on a susceptible host from juveniles hatched in S. sisymbriifolium root exudates was not affected, nor was the number of encysted eggs from progeny cysts. Transcriptome analysis by using RNA-sequencing (RNA-seq) revealed the molecular basis of root exudate-mediated modulation of nematode behavior. Differentially expressed genes are grouped into two major categories: genes showing characteristics of effectors and genes involved in stress responses and xenobiotic metabolism. To our knowledge, this is the first study that shows genome-wide root exudate-specific transcriptional changes in hatched preparasitic juveniles of plant-parasitic nematodes. This research provides a better understanding of the correlation between exudates from different plants and their impact on nematode behavior prior to the root invasion and supports the hypothesis that root exudates play an important role in plant-nematode interactions.

Published
2022

6. The potato cyst nematode effector RHA1B is a ubiquitin ligase and uses two distinct mechanisms to suppress plant immune signaling

Author

Kud, Joanna, primary, Wang, Wenjie, additional, Gross, Rachel, additional, Fan, Youhong, additional, Huang, Li, additional, Yuan, Yulin, additional, Gray, Amanda, additional, Duarte, Aida, additional, Kuhl, Joseph C., additional, Caplan, Allan, additional, Goverse, Aska, additional, Liu, Yongsheng, additional, Dandurand, Louise-Marie, additional, and Xiao, Fangming, additional

Published
2019
Full Text
View/download PDF

14. Sl NAC1, a stress-related transcription factor, is fine-tuned on both the transcriptional and the post-translational level.

Author

Huang, Weizao, Miao, Min, Kud, Joanna, Niu, Xiangli, Ouyang, Bo, Zhang, Junhong, Ye, Zhibiao, Kuhl, Joseph C., Liu, Yongsheng, and Xiao, Fangming

Subjects
GENETIC transcription in plants, TOMATO genetics, TRANSCRIPTION factors, PROTEASOME inhibitors, PSEUDOMONAS diseases
Abstract

The plant-specific NAC ( NAM, ATAF1,2, CUC2) transcription factors play significant roles in diverse physiological processes. In this study, we determined the regulation of a stress-related tomato ( Solanum lycopersicum) NAC1 ( Sl NAC1) transcription factor at both the transcriptional and the post-translational level., The Sl NAC1 protein was found to be stable in the presence of proteasome-specific inhibitor MG132 or MG115 and ubiquitinated in plant cells, suggesting that the Sl NAC1 is subject to the ubiquitin-proteasome system-mediated degradation. Deletion analysis identified a short segment of 10 amino acids (aa261-270) that was required for ubiquitin-proteasome system-mediated degradation, among which two leucine residues (L268 and L269) were critical for the protein instability of Sl NAC1. Fusion of the degron ( Sl NAC1191-270) containing these 10 amino acids to green fluorescent protein was found to be sufficient to trigger the degradation of the fusion protein., In addition, the Sl NAC1 gene is strongly upregulated during Pseudomonas infection, while repression of the NAC1 ortholog in Nicotiana benthamiana resulted in enhanced susceptibility to Pseudomonas bacteria., These results suggest that rapid upregulation of the NAC1 gene resulting in more protein production is likely one of the strategies plants use to defend themselves against pathogen infection. [ABSTRACT FROM AUTHOR]

Published
2013
Full Text
View/download PDF

15. SlNAC1, a stress-related transcription factor, is fine-tuned on both the transcriptional and the post-translational level.

Author

Huang W, Miao M, Kud J, Niu X, Ouyang B, Zhang J, Ye Z, Kuhl JC, Liu Y, and Xiao F

Subjects
Disease Resistance genetics, Gene Silencing, Solanum lycopersicum microbiology, Plant Cells metabolism, Plant Cells microbiology, Plant Diseases microbiology, Plant Proteins chemistry, Plant Proteins genetics, Plant Proteins metabolism, Pseudomonas, Sequence Analysis, Protein, Trans-Activators chemistry, Trans-Activators genetics, Trans-Activators metabolism, Ubiquitination, Gene Expression Regulation, Plant, Solanum lycopersicum genetics, Plant Proteins physiology, Trans-Activators physiology
Abstract

The plant-specific NAC (NAM, ATAF1,2, CUC2) transcription factors play significant roles in diverse physiological processes. In this study, we determined the regulation of a stress-related tomato (Solanum lycopersicum) NAC1 (SlNAC1) transcription factor at both the transcriptional and the post-translational level. The SlNAC1 protein was found to be stable in the presence of proteasome-specific inhibitor MG132 or MG115 and ubiquitinated in plant cells, suggesting that the SlNAC1 is subject to the ubiquitin-proteasome system-mediated degradation. Deletion analysis identified a short segment of 10 amino acids (aa261-270) that was required for ubiquitin-proteasome system-mediated degradation, among which two leucine residues (L268 and L269) were critical for the protein instability of SlNAC1. Fusion of the degron (SlNAC1(191-270) ) containing these 10 amino acids to green fluorescent protein was found to be sufficient to trigger the degradation of the fusion protein. In addition, the SlNAC1 gene is strongly upregulated during Pseudomonas infection, while repression of the NAC1 ortholog in Nicotiana benthamiana resulted in enhanced susceptibility to Pseudomonas bacteria. These results suggest that rapid upregulation of the NAC1 gene resulting in more protein production is likely one of the strategies plants use to defend themselves against pathogen infection., (© 2012 The Authors. New Phytologist © 2012 New Phytologist Trust.)

Published
2013
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results