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19 results on '"Kundlacz, Cindy"'

2. BTG1 inactivation drives lymphomagenesis and promotes lymphoma dissemination through activation of BCAR1

Author

Delage, Lorric, Lambert, Mireille, Bardel, Émilie, Kundlacz, Cindy, Chartoire, Dimitri, Conchon, Axel, Peugnet, Anne-Laure, Gorka, Lucas, Auberger, Patrick, Jacquel, Arnaud, Soussain, Carole, Destaing, Olivier, Delecluse, Henri-Jacques, Delecluse, Susanne, Merabet, Samir, Traverse-Glehen, Alexandra, Salles, Gilles, Bachy, Emmanuel, Billaud, Marc, Ghesquières, Hervé, Genestier, Laurent, Rouault, Jean-Pierre, and Sujobert, Pierre

Published
2023
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6. Complete genome sequence of an Enterococcus devrieseistrain isolated from Zophobas moriolarvae

Author

Kundlacz, Cindy, Eddoubaji, Yasmine, Perreten, Vincent, Endimiani, Andrea, and Campos-Madueno, Edgar I.

Abstract

Enterococcusspp. are typically found in multiple settings and are sometimes responsible for difficult-to-treat infections. In this context, very little is known about Enterococcus devriesei(E. devriesei), a rare species first isolated from a river lamprey. Importantly, no complete genome of E. devrieseicurrently exists in public repositories.

Published
2025
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12. Novel Function of Bluetongue Virus NS3 Protein in Regulation of the MAPK/ERK Signaling Pathway

Author

Kundlacz, Cindy, primary, Pourcelot, Marie, additional, Fablet, Aurore, additional, Amaral Da Silva Moraes, Rayane, additional, Léger, Thibaut, additional, Morlet, Bastien, additional, Viarouge, Cyril, additional, Sailleau, Corinne, additional, Turpaud, Mathilde, additional, Gorlier, Axel, additional, Breard, Emmanuel, additional, Lecollinet, Sylvie, additional, van Rijn, Piet A., additional, Zientara, Stephan, additional, Vitour, Damien, additional, and Caignard, Grégory, additional

Published
2019
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14. Novel function of Bleutongue Virus NS3 Protein in Regulation of the MAPK/ERK Signaling Pathway

Author

Kundlacz, Cindy, Pourcelot, Marie, Fablet, Aurore, Amaral Da Silva Moraes, Rayane, Leger, Thibaut, Morlet, Bastien, Viarouge, Cyril, Sailleau, C., Turpaud, Mathilde, Gorlier, Axel, Breard, Emmanuel, Lecollinet, S., van Rijn, P.A., Zientara, Stephan, Vitour, Damien, Caignard, Gregory, Kundlacz, Cindy, Pourcelot, Marie, Fablet, Aurore, Amaral Da Silva Moraes, Rayane, Leger, Thibaut, Morlet, Bastien, Viarouge, Cyril, Sailleau, C., Turpaud, Mathilde, Gorlier, Axel, Breard, Emmanuel, Lecollinet, S., van Rijn, P.A., Zientara, Stephan, Vitour, Damien, and Caignard, Gregory

Abstract

Bluetongue virus (BTV) is an arbovirus transmitted by blood-feeding midges to a wide range of wild and domestic ruminants. In this report, we showed that BTV, through its nonstructural protein NS3 (BTV-NS3), is able to activate the mitogen-activated protein kinase/extracellular signal-regulated kinase (MAPK/ERK) pathway, as assessed by phosphorylation levels of ERK1/2 and the translation initiation factor eukaryotic translation initiation factor 4E (eIF4E). By combining immunoprecipitation of BTV-NS3 and mass spectrometry analysis from both BTV-infected and NS3-transfected cells, we identified the serine/threonine-protein kinase B-Raf (BRAF), a crucial player in the MAPK/ERK pathway, as a new cellular interactor of BTV-NS3. BRAF silencing led to a significant decrease in the MAPK/ERK activation by BTV, supporting a model wherein BTV-NS3 interacts with BRAF to activate this signaling cascade. This positive regulation acts independently of the role of BTV-NS3 in counteracting the induction of the alpha/beta interferon response. Furthermore, the intrinsic ability of BTV-NS3 to bind BRAF and activate the MAPK/ERK pathway is conserved throughout multiple serotypes/strains but appears to be specific to BTV compared to other members of Orbivirus genus. Inhibition of MAPK/ERK pathway with U0126 reduced viral titers, suggesting that BTV manipulates this pathway for its own replication. Altogether, our data provide molecular mechanisms that unravel a new essential function of NS3 during BTV infection.

Published
2019

15. The nonstructural protein NSs of Schmallenberg virus is targeted to the nucleolus and induces nucleolar disorganization

Author

Gouzil, Julie, Fablet, Aurore, Lara, Estelle, Caignard, Grégory, Cochet, Marielle, Kundlacz, Cindy, Palmarini, Massimo, Varela, Mariana, Breard, Emmanuel, Sailleau, Corinne, Viarouge, Cyril, Coulpier, Muriel, Zientara, Stéphan, and Vitour, Damien

Abstract

Schmallenberg virus (SBV) was discovered in Germany in late 2011 and then spread rapidly to many European countries. SBV is an orthobunyavirus that causes abortion and congenital abnormalities in ruminants. A virus-encoded nonstructural protein, termed NSs, is a major virulence factor of SBV, and it is known to promote the degradation of Rpb1, a subunit of the RNA polymerase II (Pol II) complex, and therefore hampers global cellular transcription. In this study, we found that NSs is mainly localized in the nucleus of infected cells and specifically appears to target the nucleolus through a nucleolar localization signal (NoLS) localized between residues 33 and 51 of the protein. NSs colocalizes with nucleolar markers such as B23 (nucleophosmin) and fibrillarin. We observed that in SBV-infected cells, B23 undergoes a nucleolus-to-nucleoplasm redistribution, evocative of virus-induced nucleolar disruption. In contrast, the nucleolar pattern of B23 was unchanged upon infection with an SBV recombinant mutant with NSs lacking the NoLS motif (SBVΔNoLS). Interestingly, unlike wild-type SBV, the inhibitory activity of SBVΔNoLS toward RNA Pol II transcription is impaired. Overall, our results suggest that a putative link exists between NSs-induced nucleolar disruption and its inhibitory function on cellular transcription, which consequently precludes the cellular antiviral response and/or induces cell death.

Published
2017

16. Nonstructural Protein NSs of Schmallenberg Virus Is Targeted to the Nucleolus and Induces Nucleolar Disorganization

Author

Gouzil, Julie, primary, Fablet, Aurore, additional, Lara, Estelle, additional, Caignard, Grégory, additional, Cochet, Marielle, additional, Kundlacz, Cindy, additional, Palmarini, Massimo, additional, Varela, Mariana, additional, Breard, Emmanuel, additional, Sailleau, Corinne, additional, Viarouge, Cyril, additional, Coulpier, Muriel, additional, Zientara, Stéphan, additional, and Vitour, Damien, additional

Published
2017
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17. A new OCH β-lactamase from a Brucella pseudintermedia (Ochrobactrum intermedium) strain isolated from Zophobas morio larvae

Author

Kundlacz, Cindy, Aldeia, Claudia, Eddoubaji, Yasmine, Campos-Madueno, Edgar I., and Endimiani, Andrea

Abstract

•A cephalosporin-resistant Brucella pseudintermedia(Ops-OCH-23) was found in Z. moriolarvae.•The strain underwent hybrid whole-genome sequencing with both Illumina and Nanopore.•Analysis of 16S rRNA revealed 100% homology between Ops-OCH-23 and other B. pseudintermediastrains.•Core-genome analysis indicated that Ops-OCH-23 was similar to strain CTOTU49018 reported in Germany.•A new chromosomal class C OCH β-lactamase was identified in Ops-OCH-23.

Published
2023
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18. BTG1 inactivation drives lymphomagenesis and promotes lymphoma dissemination through activation of BCAR1

Author

Delage, Lorric, Lambert, Mireille, Bardel, Émilie, Kundlacz, Cindy, Chartoire, Dimitri, Conchon, Axel, Peugnet, Anne-Laure, Gorka, Lucas, Auberger, Patrick, Jacquel, Arnaud, Soussain, Carole, Destaing, Olivier, Delecluse, Henri-Jacques, Delecluse, Susanne, Merabet, Samir, Traverse-Glehen, Alexandra, Salles, Gilles, Bachy, Emmanuel, Billaud, Marc, Ghesquières, Hervé, Genestier, Laurent, Rouault, Jean-Pierre, and Sujobert, Pierre

Abstract

Understanding the functional role of mutated genes in cancer is required to translate the findings of cancer genomics into therapeutic improvement. BTG1is recurrently mutated in the MCD/C5 subtype of diffuse large B-cell lymphoma (DLBCL), which is associated with extranodal dissemination. Here, we provide evidence that Btg1knock out accelerates the development of a lethal lymphoproliferative disease driven by Bcl2 overexpression. Furthermore, we show that the scaffolding protein BCAR1 is a BTG1 partner. Moreover, after BTG1deletion or expression of BTG1mutations observed in patients with DLBCL, the overactivation of the BCAR1-RAC1 pathway confers increased migration ability in vitro and in vivo.These modifications are targetable with the SRC inhibitor dasatinib, which opens novel therapeutic opportunities in BTG1mutated DLBCL.

Published
2022
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19. Complete genome sequence of Pseudomonas canadensis strain Pcan-CK-23 isolated from Zophobas morio larvae.

Author

Kundlacz C, Aldeia C, Eddoubaji Y, Campos-Madueno EI, and Endimiani A

Abstract

We present the complete genome sequence of Pseudomonas canadensis . The strain (Pcan-CK-23) was isolated from Zophobas morio (superworm) larvae. The genome consisted of a 6,424,469 bp chromosome with a GC content of 60.3% and 5,973 genes. Pcan-CK-23 can be used as a reference genome for further studies with P. canadensis ., Competing Interests: The authors declare no conflict of interest.

Published
2024
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