Search

Your search keyword '"Kuon, Wolfgang"' showing total 15 results
Start Over Author "Kuon, Wolfgang"
15 results on '"Kuon, Wolfgang"'

8. Identification of Novel Human Aggrecan T Cell Epitopes in HLA-B27 Transgenic Mice Associated with Spondyloarthropathy

Author

Kuon, Wolfgang, primary, Kuhne, Maren, additional, Busch, Dirk H., additional, Atagunduz, Pamir, additional, Seipel, Martina, additional, Wu, Peihua, additional, Morawietz, Lars, additional, Fernahl, Gabriele, additional, Appel, Heiner, additional, Weiss, Elisabeth H., additional, Krenn, Veit, additional, and Sieper, Joachim, additional

Published
2004
Full Text
View/download PDF

10. Kaposi's Sarcoma-Associated Herpesvirus Cytotoxic T Lymphocytes Recognize and Target Darwinian Positively Selected Autologous K1 Epitopes

Author

Stebbing, Justin, primary, Bourboulia, Dimitra, additional, Johnson, Margaret, additional, Henderson, Stephen, additional, Williams, Ian, additional, Wilder, Natalie, additional, Tyrer, Mervyn, additional, Youle, Mike, additional, Imami, Nesrina, additional, Kobu, Toru, additional, Kuon, Wolfgang, additional, Sieper, Joachim, additional, Gotch, Frances, additional, and Boshoff, Chris, additional

Published
2003
Full Text
View/download PDF

11. Identification of HLA-B27-Restricted Peptides from the Chlamydia trachomatis Proteome with Possible Relevance to HLA-B27-Associated Diseases

Author

Kuon, Wolfgang, primary, Holzhütter, Hermann-Georg, additional, Appel, Heiner, additional, Grolms, Martina, additional, Kollnberger, Simon, additional, Traeder, Alexander, additional, Henklein, Peter, additional, Weiss, Elisabeth, additional, Thiel, Andreas, additional, Lauster, Roland, additional, Bowness, Paul, additional, Radbruch, Andreas, additional, Kloetzel, Peter-Michael, additional, and Sieper, Joachim, additional

Published
2001
Full Text
View/download PDF

15. Use of HLA-B27 tetramers to identify low-frequency antigen-specific T cells in Chlamydia-triggered reactive arthritis

Author

Appel, Heiner, Kuon, Wolfgang, Kuhne, Maren, Wu, Peihua, Kuhlmann, Stefanie, Kollnberger, Simon, Thiel, Andreas, Bowness, Paul, and Sieper, Joachim

Abstract

Reports of the use of HLA-B27/peptide tetrameric complexes to study peptide-specific CD8+ T cells in HLA-B27+-related diseases are rare. To establish HLA-B27 tetramers we first compared the function of HLA-B27 tetramers with HLA-A2 tetramers by using viral epitopes. HLA-B27 and HLA-A2 tetramers loaded with immunodominant peptides from Epstein–Barr virus were generated with comparable yields and both molecules detected antigen-specific CD8+ T cells. The application of HLA-B27 tetramers in HLA-B27-related diseases was performed with nine recently described Chlamydia-derived peptides in synovial fluid and peripheral blood, to examine the CD8+ T cell response against Chlamydia trachomatis antigens in nine patients with Chlamydia-triggered reactive arthritis (Ct-ReA). Four of six HLA-B27+ Ct-ReA patients had specific synovial T cell binding to at least one HLA-B27/Chlamydia peptide tetramer. The HLA-B27/Chlamydia peptide 195 tetramer bound to synovial T cells from three of six patients and HLA-B27/Chlamydia peptide 133 tetramer to synovial T cells from two patients. However, the frequency of these cells was low (0.02–0.09%). Moreover, we demonstrate two methods to generate HLA-B27-restricted T cell lines. First, HLA-B27 tetramers and magnetic beads were used to sort antigen-specific CD8+ T cells. Second, Chlamydia-infected dendritic cells were used to stimulate CD8+ T cells ex vivo. Highly pure CD8 T cell lines could be generated ex vivo by magnetic sorting by using HLA-B27 tetramers loaded with an EBV peptide. The frequency of Chlamydia-specific, HLA-B27 tetramer-binding CD8+ T cells could be increased by stimulating CD8+ T cells ex vivo with Chlamydia-infected dendritic cells. We conclude that HLA-B27 tetramers are a useful tool for the detection and expansion of HLA-B27-restricted CD8+ T cells. T cells specific for one or more of three Chlamydia-derived peptides were found at low frequency in synovial fluid from HLA-B27+ patients with Ct-ReA. These cells can be expanded ex vivo, suggesting that they are immunologically functional.

Published
2004
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results