Search

In the present study, aqueous leaf extract of Strobilanthes cordifolia J.R.I.Wood was combined with silver nitrate to synthesis silver nanoparticles (AgNPs). The AgNPs were characterized using visible spectroscopy (UV), X-ray diffraction (XRD), Fourier transform infrared spectrophotometer (FTIR), scanning electron microscope (SEM), energy dispersive X-ray (EDaX), particle size analysis, and transmission electron microscope (TEM). The UV spectrum absorption peak occurred at 438 nm. The FTIR analysis of the AgNPs indicated the presence of functional groups such as aldehyde, alkenes, and carboxylic acids. The crystalline structure of AgNPs was confirmed by XRD. The AgNPs have a spherical shape according to SEM. The AgNPs components composition was confirmed by EDaX. The particle size distribution of AgNPs is monodispersion in the range at 42.54 nm. TEM demonstrated the AgNPs size to be between 11.35 and 34.90 nm. The AgNPs exhibited good antibacterial property against Escherichia coli and Staphylococcus aureus. The antioxidant activity of the AgNPs was represented by increased DPPH, ABTS, and H

Hepatocellular carcinoma (HCC) is the most common primary liver cancer, with a rising worldwide burden due to a lack of efficient treatment techniques and diagnosis after it has metastasized. Therefore, small non-coding RNA (miRNAs) as protein translation inhibitors are gaining attention that degrades or suppress specific gene transcripts, making it a prime strategy for oncogenes or tumor suppression. Systematic research with miRNAs in combination with Arsenic, which has been employed as a drug to treat several diseases, including cancer, was focused on cellular responses through interacting with multiple biological targets. The differential gene expression of the DNA microarray dataset (GSE48441) revealed the association of sterol, cholesterol, and lipid metabolic processes. With the aid of the network pharmacology approach, hsa-mir-335-5p was uncovered to negatively regulate the important nodes driving the transport and utilization of essential compounds for the rapid growth and proliferation of cancer cells. The binding energies of the duplexes were validated by the minimal free energies of the mRNAs for hsa-mir-335-5p, indicating energetically desirable binding association. The molecular interactions between hsa-mir-335-5p, which interacts with the Argonaute protein in the RNA induced silencing complex, and the target-specific genes were also investigated, revealing its susceptibility to be employed in in vitro studies.

Objective: Current scientific investigations have evidenced that there is increased attention in developing novel drugs from bio source for the human health concern. This study's main objective was to intervene phytochemical constituents present in the ethanol leaf extract of an aromatic plant, Ixora brachiata (Roxb) and to evaluate their ability on antioxidant, anticancer and larvicidal properties, using invitro models. Methods: Antioxidant assay was performed by scavenging behaviour of DPPH, ABTS, H2 O2 and Reducing power screenings. The phytochemical composition was also screened using GC-MS technique. Cytotoxicity effect was determined by employing MTT Assay, the larvicidal potential was predicted by following the protocol described by WHO. In phytochemical screening higher concentration of alkaloids, flavonoids, phenolic and diterpenoids were detected in significant content. Geranyl linalool was the profound compound present, and it has been reported, used in various treatments for cancer ailments, similar to Taxol. Results: The cytotoxicity values of ethanol extract were found to be 11.57 to 55.95% against the cancer cells (A549) and 12.36-63.8% range was observed against HeLa cancers cell line. Larvicidal effects of the extract showed minimum LC50 value 14.69mg/ml against Aedes aegypti and 10.43mg/ml was recorded, against Culex quinquefasciatus, indicating, a promising capability of larvicidal action against mosquito larvae. Conclusions: This study envisaged that ethanol extracts of I.brachiata are a bright and capable therapeutic agent, to combat in the battle of life threatened diseases, and infectious emerging threats.

Natural products from plants, animals and minerals are the basis for treating human diseases. The present investigation explains that the antioxidant potential of aqueous extract of vitex negundo leaves. Phytochemical constituents, total phenolic and flavanoid content of aqueous analysis and vitro methods such as 1,1-diphenyl-2picrylhydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) radical scavenging assays and GC_MS studies are reported. In addition, TPC and TFC of the extract were evaluated. It was observed that the leaf extract total phenol (14.3 mg) and flavanoids (8.5 mg) Quercetin equivalent (Q/g) high level of Phenolic and flavonoid content that might have accounted for the strong activity observed ABTS 62% of inhibition µg/ mL almost equivalent to that of standard vitamin C and DPPH value of 66.32 µg/ mL almost equivalent to that of standard vitamin C results revealed that leaves of vitex negundo have Since this investigation is a preliminary study, a detailed study of the antioxidant mechanisms of specific phenol components is an absolute necessity. Many phytochemical constituents which may be responsible for many pharmacological activities.

Knoxia sumatrensis leaf extracts in methanol were analyzed for the phytochemical compositions, antioxidant properties (DPPH, ABTS, and hydroxyl radical assay), anti-proliferative activity (lung can...

To find new sources of antimicrobial and antioxidant agents, methanol extracts of Hardwickia binata Roxb were evaluated systematically. To the present investigation for potential antibacterial and antioxidant phytochemicals in this study and also ADMET and molecular docking for CADD studies. Our results the polyphenol content (total phenol, total flavonoid) and antioxidant capacity of methanol extracts were examined. Identify skin cancer active sites from Hardwickia binata bioactive compounds using GCMS and molecular docking, network pharmacology. The free radical scavenging activities of the methanol extracts also were highest, with the antioxidant activity becoming significantly greater. Furthermore, in vitro antibacterial experiments against phytopathogens such as Staphylococcus aureus, Enterococcus faecalis, Escherichia coli and Pseudomonas aureginosa validated LE's antimicrobial efficacy. Compared to the other pathogens Enterococcus faecalis have high zone of inhibition (14 ± 0.54). Chemical composition of crude drug extracts was also performed by identify functional groups such as N–H, C–H, OH, and C = O in the methanol extract. GC-MS investigation revealed the existence of 30 bioactive chemicals and 7 active molecules were identify with ADMET and docking in skin cancer proteins (1P7K and 5OTE) among the phytocompounds, Quinoline, 1,2-dihydro-2,2,4-trimethyl (-4.2 kcal/mol; -4.6 kcal/mol), Hexadecanoic acid, methyl ester (-6. kcal/mol; -6.2 kcal/mol) and 5-Phenyl-2,4-pyrimidinediamine, 2TMS derivative (-8.11 kcal/mol; -7.50 kcal/mol) Hardwickia binata is the best compound for the human skin cancer possessed higher binding energy. Our results indicate that the plants can provide sources of natural compounds and the docking studies were carried out in order to predict the most probable kind of interaction, binding affinities, and orientations of the docked ligands at the target protein's active site.

In around the world, mosquito control is considered a most important because of the incapable of synthetic insecticides and the ecological pollution about by them. In this manner, need the eco-friendly insecticides to efficient control the mosquito disease is the need of the hour. We synthesized the eco-friendly of zinc oxide nanoparticles (ZnO-NPs) using the Knoxia sumatrensis aqueous leaf extract (Ks-ALE) as a reducing and stabilizing agent. The synthesis of ZnO-NPs was confirmed by UV with an absorption peak at 354 nm. ZnO-NPs crystal structure was analyzed by X-ray diffraction (XRD). Fourier transform infrared spectroscopy (FT-IR) spectra revealed the chloride, cyclic alcohols, sulfonamies, carboxylic acids, oximes, phosphines, alkenes and alcohol & phenol. Field emission-scanning electron microscopy (FE-SEM) showed that the NP’s are rod shaped with 50–80 nm size and also energy dispersive spectra (EDaX) spectra showed presence of zinc. Antioxidant assay showed superior activity and evidenced by DPPH, ABTS and H2O2 radical assays. Furthermore, the ZnO-NPs exhibited strong activity in MCF-7 cell line with IC50 value is 58.87 μg/mL. Mosquito larvicidal activity of ZnO-NPs produced significant activity and excellent larvicidal activity was noticed in Cx. quinquefasciatus with LC50 0.08, mg/mL and LC9019.46 mg/mL. This study suggests that synthesized ZnO-NPs using Knoxia sumatrensis leaf extract have good biological activities and it makes them an ideal candidate for pharmacological studies.

This study describes the antiproliferative activity of thiocolchicoside and colchicine compounds in a dose-dependent manner against breast cancer cell line (MCF-7). MTT assay results show that IC50...

Increased industrialization demand using synthetic dyes in the newspaper, cosmetics, textiles, food, and leather industries. As a consequence, harmful chemicals from dye industries are released into water reservoirs with numerous structural components of synthetic dyes, which are hazardous to the ecosystem, plants and humans. The discharge of synthetic dye into various aquatic environments has a detrimental effect on the balance and integrity of ecological systems. Moreover, numerous inorganic dyes exhibit tolerance to degradation and repair by natural and conventional processes. So, the present condition requires the development of efficient and effective waste management systems that do not exacerbate environmental stress or endanger other living forms. Numerous biological systems, including microbes and plants, have been studied for their ability to metabolize dyestuffs. To minimize environmental impact, bioremediation uses endophytic bacteria, which are plant beneficial bacteria that dwell within plants and may improve plant development in both normal and stressful environments. Moreover, Phytoremediation is suitable for treating dye contaminants produced from a wide range of sources. This review article proves a comprehensive evaluation of the most frequently utilized plant and microbes as dye removal technologies from dye-containing industrial effluents. Furthermore, this study examines current existing technologies and proposes a more efficient, cost-effective method for dye removal and decolorization on a big scale. This study also aims to focus on advanced degradation techniques combined with biological approaches, well regarded as extremely effective treatments for recalcitrant wastewater, with the greatest industrial potential.

Plant-derived antioxidants are a large group of natural products with the capacity to reduce radical-scavenging. Due to their potent therapeutic and preventive actions, these compounds receive a lot of attention from scientists, particularly pharmacologists. The pharmacological activities of the Azima tetracantha Lam. (AT) plant, belonging to the Salvadoraceae family, reported here justifies its traditional use in treating several diseases or disorders. This study aims to look at the propensity of certain plant compounds found in natural AT plant extracts that might play a critical role as a secondary metabolite in cervical cancer treatment. There is a shortage of information on the plant’s phytochemical and biological characteristics. Methanol (MeOH) solvent extracts of the dried AT plant were screened phytochemically. Its aqueous extract was tested for antioxidant, antiseptic, anti-inflammatory, and anticancerous properties. Absorption Distribution Metabolism and Excretion (ADME/T), Docking, and HPLC were also performed. In clinical treatment, the plant shown no adverse effects. The antioxidant activity was evaluated and showed the highest concentration at 150 µg/mL (63.50%). MeOH leaf extract of AT exhibited the highest and best inhibitory activity against Staphylococcus aureus (15.3 mm/1000) and displayed a high antiseptic potential. At a 200 µg/mL concentration, MeOH leaves-extract inhibited red blood cells (RBC) hemolysis by 66.56 ± 0.40, compared with 62.33 ± 0.40 from the standard. Albumin’s ability to suppress protein denaturation ranged from 16.75 ± 0.65 to 62.35 ± 0.20 inhibitions in this test, providing even more support for its favorable anti-inflammatory properties. The ADME/T studies were considered for a potential cancer drug molecule, and one of our compounds from MeOH extract fills the ADME and toxicity parameters. The forms of compound 4 showed a strong hydrogen-bonding interaction with the vital amino acids (ASN923, THR410, LEU840TRY927, PHE921, and GLY922). A total of 90% of cell inhibition was observed when HeLa cell lines were treated with 300 µg/mL of compound 4 (7-acetyl-3a1-methyl- 4,14-dioxo-1,2,3a,3a1,4,5,5a,6,8a,9b,10,11,11a-tetradecahydro-2,5a epoxy5,6a (methanooxymethano)phenaleno[1′,9′:5,6,7]indeno[1,7a-b]oxiren-2-yl acetate). The polyphenol compounds demonstrated significant advances in anticancer drug properties, and it could lead to activation of cancer cell apoptosis.

Chitosan derived silver biocomposites (CsS) were produced by green synthesis using Carmona retusa (Vahl) Masam aqueous leaf extract. UV–Vis spectra of synthesized CsS biocomposites showed absorption maxima at 441 nm. FESEM average particle size was 51 nm and spherical in shape. TEM images of CsS biocomposite ranged between 30 nm to 60 nm, the DLS measurement showed the size of 234.1 nm for chitosan derived AgNPs. In FTIR spectra, the C–H winding and were observed for CsS biocomposites. In addition, the elemental composition showed uniform grain boundaries as recognized by EDaX spectra. In-vitro antioxidant activity CsS biocomposites showed the ability to scavenge free radicals. Cytotoxicity analysis of CsS biocomposites on MCF-7 breast cancer cell line revealed 90% inhibition at 500 μg/ml concentration. C. retusa mediated synthesis AgNPs coated chitosan biocomposite showed strong larvicidal activity with low LC50 and LC90 values against the malarial vector, An. stephensi, Ae. aegypti, and Cx. quinquefasciatus respectively. Eight bioactive components were present in aqueous leaf extracts of C. retusa. Based on this study we suggest that C. retusa plant mediated AgNPs chitosan derived silver biocomposites (CsS) has anti-cancerous and insecticidal activity which can further be explored for commercialization.

We studied the pharmacological potential effects of viral components, performed target prediction and network analysis, and investigated interactive pathways utilizing a pharmacology approach to molecular docking and dynamics and network pharmacology. The radical scavenging activity was undertaken to evaluate the effect of substituent on the antioxidant activities of the all-synthesized compounds and shows promising activity. A benefit of utilizing phytochemicals contain arrangements becomes the excellent patient safety with little negative effects. The compounds (Trans-13-Octadecenoic-acid −188.24 kal/mol (ARG 431, GLN 470, ARG 431, SER 433, TYR 458 and ARG 431). PASS prediction screening results for bioactive compounds of trust standard for human intestinal absorption and penetration of the blood-brain barrier. Likewise, another predicate also has a 0.816% level of confidence toward intestinal absorption 95% level of confidence for BBB penetration using viral trials of 5-Hydroxymethyl furfural is a feasible key to the design of antiviral therapies for viral protease inhibition. In designing novel antiviral medicines, the recognized pharmacophore structures of bioactive compounds may be valuable. A benefit of utilizing phytochemicals containing preparations becomes the excellent patient safety with little negative effects. In designing novel antiviral medicines, the recognized pharmacophore structures of bioactive compounds may be useful.

Plants produced natural generating products play a significant role in drug discovery of new bioactive compounds and these are used for advancement of innovative curative drugs for specific target health diseases. In this study Docking and ADME/T virtual screening method are apply for in drug discovery and can be divided into ligand- and target structure-based. The aim of this study was to analyze the

Mosquito control is facing a challenge worldwide, due to ineffectiveness of chemical insecticides. Identification of novel and ecofriendly insecticides is the need of the hour. In the present study mosquito control efficacy, antioxidants and anti-cancerous potential of silver nanoparticle conjugated with Carmona retusa (Vahl) Masam nanoparticles. Synthesized nanoparticles had UV absorption maximum at 420 nm, size ranging between 20 and 40 nm based of TEM, has cubic structure, C–H bending and Ag Metal bands. Results show high free radical scavenging ability of C. retusa derived silver nanoparticles as evidenced by DPPH radical and H2O2 radical assay. C. retusa derived nanoparticles produce 80% inhibition in MCF-7 cell line at concentration of 500 μg/ml. High larvicidal activity with LC50 values of 116.681 ppm for Anopheles stephensi, 198.766 ppm for Aedes aegypti, and 83.553 ppm for Culex quinquefasciatus were observed. Based on the findings of the study we suggest that C. retusa plant mediated AgNPs has anticancerous and mosquito larvicidal property and could be bioprospected for drug development and mosquito control.

Objective Abutilon indicum is a medicinal plant belonging to the Malvaceae family. The current study has been developed to detect Abutilon indicum bio-activity to produce an adequate drug design for cancer. Methods The objective of this work is to perform molecular docking and dynamics as well as inhibitors and cancer cell line studies of Abutilon indicum would be essentially effective to use current strong medicines from oncology therapies. Results By Docking best finding binding energy -12.02kcal/mol (ARG310, ASP323, SER291, THR358, GLU293) amino acid has been found to be immersed in the formation of the hydrogen interaction. This finding also indicates that a range of compounds are ADMET positive drug molecules in cancer studies. Network pharmacology showed that the signal rule ERG, PTEN, NKX31, AR, ETV4, STAT3, PTPN11, CBL, KRAS, EREG, STAT3, GRB2, HRAS, and SHC1, and the axis of DRD2. Molecular simulation trajectories show that RMS deviation profiles were relatively stable during the simulation and it indicated the orientations were created by the docking studies. In cell lines MCF-7, the active compound R-N-1'-methoxycarbonyl-2'-phenylethyl-4-hydroxy benzamide has anticancer inhibitory 76.56% at 100μg/mL. The ASP323 interaction of EGFR inhibitors interaction molecules were derived that can be successfully used to explain the cancer activities. Conclusion The results of pharmacodynamic and toxicity for natural organic derived compound and its active results epidermal growth factor receptor for identifying novel drugs for the treatment confirms compound moderate to a good cancer drug.