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Your search keyword '"Kuraoka, Masayuki"' showing total 152 results
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152 results on '"Kuraoka, Masayuki"'

1. An epitope-enriched immunogen expands responses to a conserved viral site

Author

Caradonna, Timothy M., Ronsard, Larance, Yousif, Ashraf S., Windsor, Ian W., Hecht, Rachel, Bracamonte-Moreno, Thalia, Roffler, Anne A., Maron, Max J., Maurer, Daniel P., Feldman, Jared, Marchiori, Elisa, Barnes, Ralston M., Rohrer, Daniel, Lonberg, Nils, Oguin, Thomas H., III, Sempowski, Gregory D., Kepler, Thomas B., Kuraoka, Masayuki, Lingwood, Daniel, and Schmidt, Aaron G.

Published
2022
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2. A non-neutralizing glycoprotein B monoclonal antibody protects against herpes simplex virus disease in mice

Author

Kuraoka, Masayuki, Aschner, Clare Burn, Windsor, Ian W., Mahant, Aakash Mahant, Garforth, Scott J., Kong, Susan Luozheng, Achkar, Jacqueline M., Almo, Steven C., Kelsoe, Garnett, and Herold, Betsy C.

Subjects
Glycoproteins -- Health aspects, Herpes simplex -- Drug therapy, Herpesvirus vaccines -- Research, Pharmaceutical research, Monoclonal antibodies -- Research, Health care industry
Abstract

There is an unmet need for monoclonal antibodies (mAbs) for prevention or as adjunctive treatment of herpes simplex virus (HSV) disease. Most vaccine and mAb efforts focus on neutralizing antibodies, but for HSV this strategy has proven ineffective. Preclinical studies with a candidate HSV vaccine strain, [DELTA]gD-2, demonstrated that non-neutralizing antibodies that activate Fc[gamma] receptors (Fc[gamma]Rs) to mediate antibody-dependent cellular cytotoxicity (ADCC) provide active and passive protection against HSV-1 and HSV-2. We hypothesized that this vaccine provides a tool to identify and characterize protective mAbs. We isolated HSV-specific mAbs from germinal center and memory B cells and bone marrow plasmacytes of [DELTA]gD-2- vaccinated mice and evaluated these mAbs for binding, neutralizing, and Fc[gamma]R-activating activity and for protective efficacy in mice. The most potent protective mAb, BMPC-23, was not neutralizing but activated murine Fc[gamma]RIV, a biomarker of ADCC. The cryo-electron microscopic structure of the Fab-glycoprotein B (gB) assembly identified domain IV of gB as the epitope. A single dose of BMPC-23 administered 24 hours before or after viral challenge provided significant protection when configured as mouse IgG2c and protected mice expressing human Fc[gamma]RIII when engineered as a human IgG1. These results highlight the importance of FcR-activating antibodies in protecting against HSV., Introduction Before the SARS-CoV-2 pandemic, few monoclonal antibodies (mAbs) had advanced into the clinic for treatment or prevention of infectious diseases, with the notable exception of palivizumab for respiratory syncytial [...]

Published
2023
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3. Protective human antibodies against a conserved epitope in pre- and postfusion influenza hemagglutinin

Author

Finney, Joel, primary, Moseman, Annie Park, additional, Kong, Susan, additional, Watanabe, Akiko, additional, Song, Shengli, additional, Walsh, Richard M., additional, Kuraoka, Masayuki, additional, Kotaki, Ryutaro, additional, Moseman, E. Ashley, additional, McCarthy, Kevin R., additional, Liao, Dongmei, additional, Liang, Xiaoe, additional, Nie, Xiaoyan, additional, Lavidor, Olivia, additional, Abbott, Richard, additional, Harrison, Stephen C., additional, and Kelsoe, Garnett, additional

Published
2023
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6. Immune checkpoint modulation enhances HIV-1 antibody induction

Author

Bradley, Todd, Kuraoka, Masayuki, Yeh, Chen-Hao, Tian, Ming, Chen, Huan, Cain, Derek W., Chen, Xuejun, Cheng, Cheng, Ellebedy, Ali H., Parks, Robert, Barr, Maggie, Sutherland, Laura L., Scearce, Richard M., Bowman, Cindy M., Bouton-Verville, Hilary, Santra, Sampa, Wiehe, Kevin, Lewis, Mark G., Ogbe, Ane, Borrow, Persephone, Montefiori, David, Bonsignori, Mattia, Anthony Moody, M., Verkoczy, Laurent, Saunders, Kevin O., Ahmed, Rafi, Mascola, John R., Kelsoe, Garnett, Alt, Frederick W., and Haynes, Barton F.

Published
2020
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10. Infant Antibody Repertoires during the First Two Years of Influenza Vaccination

Author

Kuraoka, Masayuki, primary, Curtis, Nicholas C., additional, Watanabe, Akiko, additional, Tanno, Hidetaka, additional, Shin, Seungmin, additional, Ye, Kevin, additional, Macdonald, Elizabeth, additional, Lavidor, Olivia, additional, Kong, Susan, additional, Von Holle, Tarra, additional, Windsor, Ian, additional, Ippolito, Gregory C., additional, Georgiou, George, additional, Walter, Emmanuel B., additional, Kelsoe, Garnett, additional, Harrison, Stephen C., additional, Moody, M. Anthony, additional, Bajic, Goran, additional, and Lee, Jiwon, additional

Published
2022
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23. Immune-Focusing Properties of Virus-like Particles Improve Protective IgA Responses

Author

Onodera, Taishi, primary, Hashi, Kana, additional, Shukla, Rajni Kant, additional, Miki, Motohiro, additional, Takai-Todaka, Reiko, additional, Fujimoto, Akira, additional, Kuraoka, Masayuki, additional, Miyoshi, Tatsuya, additional, Kobayashi, Kazuo, additional, Hasegawa, Hideki, additional, Ato, Manabu, additional, Kelsoe, Garnett, additional, Katayama, Kazuhiko, additional, and Takahashi, Yoshimasa, additional

Published
2019
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28. Self-tolerance curtails the B cell repertoire to microbial epitopes

Author

Watanabe, Akiko, primary, Su, Kuei-Ying, additional, Kuraoka, Masayuki, additional, Yang, Guang, additional, Reynolds, Alexander E., additional, Schmidt, Aaron G., additional, Harrison, Stephen C., additional, Haynes, Barton F., additional, St. Clair, E. William, additional, and Kelsoe, Garnett, additional

Published
2019
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29. Antibodies to a Conserved Influenza Head Interface Epitope Protect by an IgG Subtype-Dependent Mechanism

Author

Watanabe, Akiko, primary, McCarthy, Kevin R., additional, Kuraoka, Masayuki, additional, Schmidt, Aaron G., additional, Adachi, Yu, additional, Onodera, Taishi, additional, Tonouchi, Keisuke, additional, Caradonna, Timothy M., additional, Bajic, Goran, additional, Song, Shengli, additional, McGee, Charles E., additional, Sempowski, Gregory D., additional, Feng, Feng, additional, Urick, Patricia, additional, Kepler, Thomas B., additional, Takahashi, Yoshimasa, additional, Harrison, Stephen C., additional, and Kelsoe, Garnett, additional

Published
2019
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31. 3Conserved cryptic recombination signals in Vκ gene segments are cleaved in small pre-B cells

Author

Kelsoe Garnett, Davila Marco, Kuraoka Masayuki, Lieberman Anne E, and Cowell Lindsay G

Subjects
Immunologic diseases. Allergy, RC581-607
Abstract

Abstract Background The cleavage of recombination signals (RS) at the boundaries of immunoglobulin V, D, and J gene segments initiates the somatic generation of the antigen receptor genes expressed by B lymphocytes. RS contain a conserved heptamer and nonamer motif separated by non-conserved spacers of 12 or 23 nucleotides. Under physiologic conditions, V(D)J recombination follows the "12/23 rule" to assemble functional antigen-receptor genes, i.e., cleavage and recombination occur only between RS with dissimilar spacer types. Functional, cryptic RS (cRS) have been identified in VH gene segments; these VH cRS were hypothesized to facilitate self-tolerance by mediating VH → VHDJH replacements. At the Igκ locus, however, secondary, de novo rearrangements can delete autoreactive VκJκ joins. Thus, under the hypothesis that V-embedded cRS are conserved to facilitate self-tolerance by mediating V-replacement rearrangements, there would be little selection for Vκ cRS. Recent studies have demonstrated that VH cRS cleavage is only modestly more efficient than V(D)J recombination in violation of the 12/23 rule and first occurs in pro-B cells unable to interact with exogenous antigens. These results are inconsistent with a model of cRS cleavage during autoreactivity-induced VH gene replacement. Results To test the hypothesis that cRS are absent from Vκ gene segments, a corollary of the hypothesis that the need for tolerizing VH replacements is responsible for the selection pressure to maintain VH cRS, we searched for cRS in mouse Vκ gene segments using a statistical model of RS. Scans of 135 mouse Vκ gene segments revealed highly conserved cRS that were shown to be cleaved in the 103/BCL2 cell line and mouse bone marrow B cells. Analogous to results for VH cRS, we find that Vκ cRS are conserved at multiple locations in Vκ gene segments and are cleaved in pre-B cells. Conclusion Our results, together with those for VH cRS, support a model of cRS cleavage in which cleavage is independent of BCR-specificity. Our results are inconsistent with the hypothesis that cRS are conserved solely to support receptor editing. The extent to which these sequences are conserved, and their pattern of conservation, suggest that they may serve an as yet unidentified purpose.

Published
2009
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32. Efficient Culture of Human Naïve and Memory B cells for Use as Antigen-presenting Cells

Author

Su, Kuei-Ying, Watanabe, Akiko, Yeh, Chen-Hao, Kelsoe, Garnett, and Kuraoka, Masayuki

Subjects
CD4-Positive T-Lymphocytes, B-Lymphocytes, Cell Culture Techniques, Receptors, Antigen, T-Cell, Antigen-Presenting Cells, Lymphocyte Activation, Article, Cell Line, CD4 Antigens, Tetanus Toxoid, Humans, Immunologic Memory, Cells, Cultured, Cell Proliferation
Abstract

The ability to culture and expand B cells in vitro has become a useful tool for studying human immunity. A limitation of current methods for human B-cell culture is the capacity to support mature B-cell proliferation. We have developed a culture method to support the efficient activation and proliferation of both naïve and memory human B cells. This culture supports extensive B-cell proliferation, with approximately 103-fold increases following 8 days in culture, and 106-fold increases when cultures are split and cultured for 8 more days. In culture, a significant fraction of naïve B cells undergo isotype switching and differentiate into plasmacytes. Culture-derived (CD) B cells are readily cryopreserved, and when recovered, retain their ability to proliferate and differentiate. Significantly, proliferating CD B cells express high levels of MHCII, CD80, and CD86. CD B cells act as APCs and present both alloantigens and microbial antigens to T cells. We are able to activate and expand antigen-specific memory B cells; these cultured cells are highly effective in presenting antigen to T cells. We have characterized the TCR repertoire of rare antigen-specific CD4+ T cells that proliferated in response to tetanus toxoid (TT) presented by autologous CD B cells. TCR Vβ usage by TT-activated CD4+ T cells differs from both resting and unspecifically activated CD4+ T cells. Moreover, we found that TT-specific TCR Vβ usage by CD4+ T cells was substantially different between donors. This culture method provides a platform for studying the BCR and TCR repertoires within a single individual.

Published
2016

38. Immunodominance of Antibody Recognition of the HIV Envelope V2 Region in Ig-Humanized Mice

Author

Wiehe, Kevin, primary, Nicely, Nathan I., additional, Lockwood, Bradley, additional, Kuraoka, Masayuki, additional, Anasti, Kara, additional, Arora, Sabrina, additional, Bowman, Cindy M., additional, Stolarchuk, Christina, additional, Parks, Robert, additional, Lloyd, Krissey E., additional, Xia, Shi-Mao, additional, Duffy, Ryan, additional, Shen, Xiaoying, additional, Kyratsous, Christos A., additional, Macdonald, Lynn E., additional, Murphy, Andrew J., additional, Scearce, Richard M., additional, Moody, M. Anthony, additional, Alam, S. Munir, additional, Verkoczy, Laurent, additional, Tomaras, Georgia D., additional, Kelsoe, Garnett, additional, and Haynes, Barton F., additional

Published
2017
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48. AID Expression and Activity in the Absence of Germinal Centers: Insights into Hyper-IgM Syndrome1

Author

Kuraoka, Masayuki, Liao, Dongmei, Yang, Kaiyong, Allgood, Sallie D., Levesque, Marc C., Kelsoe, Garnett, and Ueda, Yoshihiro

Subjects
Mice, Knockout, Stem Cells, Molecular Sequence Data, B-Lymphocyte Subsets, Gene Expression Regulation, Developmental, Receptors, Antigen, B-Cell, Bone Marrow Cells, Germinal Center, Hyper-IgM Immunodeficiency Syndrome, Lymphocyte Activation, Article, Immunophenotyping, Mice, Inbred C57BL, Mice, Cell Line, Tumor, Cytidine Deaminase, Animals, Humans, Female, Somatic Hypermutation, Immunoglobulin, Gene Rearrangement, B-Lymphocyte, Cell Line, Transformed, Signal Transduction
Abstract

Somatic hypermutation normally occurs as a consequence of the expression of activation-induced cytidine deaminase (AID) by Ag-activated, mature B cells during T cell-dependent germinal center responses. Nonetheless, despite their inability to express CD154 and initiate GC responses, patients with type 1 hyper-IgM syndrome (HIGM1) support populations of IgM(+)IgD(+)CD27(+) B cells that express mutated Ig genes. The origin of these mutated B cells is unknown; the IgM(+)IgD(+)CD27(+) cells do not express AID and appear to acquire mutations independent of stringent selection by Ag. Here, we demonstrate that immature/transitional 1 B cells from the bone marrow of CD154-deficient mice express AID and acquire Ig mutations that lack the hallmarks of antigenic selection via BCR signaling. Comparable levels of AID expression was found in developmentally immature B cells recovered from murine fetal liver and from human immature/transitional 1 B cells recovered from umbilical cord blood. AID expression in human fetal liver was also robust, approaching that of human tonsil tissue and the human germinal center B cell line, Ramos. These observations led us to conclude that AID expression in developing human B cells is the origin of the mutated IgM(+)IgD(+)CD27(+) B cells present in HIGM1 patients, and we propose that both mice and humans share a latent, AID-dependent pathway for the preimmune diversification of B lymphocytes that is more prominent in chicken, sheep, and rabbits.

Published
2009

49. IL-1RI dependent HSC proliferation is necessary for Inflammatory Granulopoiesis and Reactive Neutrophilia1

Author

Ueda, Yoshihiro, Cain, Derek W., Kuraoka, Masayuki, Kondo, Motonari, and Kelsoe, Garnett

Subjects
Inflammation, Mice, Knockout, Neutrophils, Multipotent Stem Cells, Receptors, Interleukin-1, hemic and immune systems, Cell Differentiation, Flow Cytometry, Hematopoietic Stem Cells, Polymerase Chain Reaction, Article, Hematopoiesis, Mice, Adjuvants, Immunologic, Alum Compounds, Animals, RNA, Messenger, Cell Proliferation, Granulocytes, Interleukin-1
Abstract

Infections and inflammation trigger neutrophilias that are supported by a hematopoietic program of accelerated granulopoiesis known as emergency granulopoiesis. The intrinsic factors that drive reactive neutrophilias and emergency granulopoiesis have been inferred but not demonstrated. Here, we show that alum cannot elicit reactive neutrophilias in IL-1R type I (IL-1RI)(-/-) mice, whereas other inflammatory responses, including eosinophilia and Ab production, remain intact. Analysis of this specific impairment revealed an unanticipated role for IL-1RI in supporting increased proliferation by granulocyte/macrophage progenitors and, surprisingly, multipotent progenitors and hematopoietic stem cells (HSC). Indeed, HSC and multipotent progenitor proliferative responses were most suppressed in IL-1RI(-/-) mice, suggesting a critical role for their proliferation in inflammatory granulopoiesis. Whereas IL-1 drives increased HSC proliferation directly in vitro, IL-1RI expression by radiation-resistant host cells was both necessary and sufficient for alum-induced HSC, multipotent progenitor, and granulocyte/macrophage progenitor proliferation and reactive neutrophilias in radiation chimeric mice. Thus, IL-1 plays a necessary, but indirect, role in the support of alum-induced neutrophilias by expanding both pluripotent and myeloid progenitor compartments to accelerate granulopoiesis.

Published
2009

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