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As an endocrine disruptor chemical, pesticide exposure may affect the regulation of growth hormones such as insulin-like growth factor-1 (IGF-1). A few current studies to date have noted that long-term pesticide exposure disrupted IGF-1, a potential risk of stunting in children. This study aims to evaluate studies to date of the effect of pesticide exposure on stunting incidence. This systematic review and meta-analysis adhered to the PRISMA (Preferred Reporting Items for Systematic Review and Meta-Analysis) guidelines. The PubMed and EBSCO databases were searched for relevant articles without publication restrictions. This review aimed to include reviews, randomized controlled trials (RCT), and cohort, case-control, and cross-sectional studies, which provide actual exposure types of pesticides with stunting measurement by height-age z score. A screening, extraction, and synthesis were conducted, leading to a consensus for reaching mutual agreement. The analysis was performed using Microsoft Excel 2017 for the screening and extraction, Revman version 5.4 software for the meta-analysis, and OpenMEE software for the meta-regression. Of the 13 studies subjected to the qualitative analysis, 6 were eligible for inclusion in the meta-analysis: 2 reviews, 2 RCTs, 2 cohorts, 2 case-control, and 5 cross-sectional studies. Exposure to organophosphate and pyrethroid pesticides was not associated with stunting (P=0.78; odds ratio [OR], 1.11; 95% confidence interval [CI], 0.65–1.88). Heterogeneity existed for 79% of the meta-analysis (P≤0.000; z=-5.37; 95% CI, -0.034 to -0.016), and the meta-regression identified age as the causative covariate. Pesticide exposure, regardless of type, is not associated with stunting in children.

Background: Anemia, commonly known as a deficiency in blood, influences individual behavior. Higher knowledge about anemia before conception correlates with a greater awareness of nutritional needs. Preventing anemia in preconception women involves iron tablet consumption, while dates serve as an alternative means to prevent anemia. This study aims to ascertain the influence of anemia knowledge, date consumption, and iron tablet intake on hemoglobin levels among prospective pregnant women at the Klaten Selatan Community Health Center. This research heald on January to September 2023 in the operational area of the Klaten Selatan Community Health Center. Methods: This research adopts a pre-experimental design employing a one- group pretest-posttest study. Purposive Random Sampling method is used, encompassing 40 research subjects. The dependent variable is hemoglobin levels, while the independent variables are knowledge about anemia, date consumption, and iron tablets. Data collection involves primary data through questionnaire responses and observation sheets. Research analysis includes univariate analysis, bivariate analysis utilizing the Spearman Rho test for knowledge variables and the Friedman test for date and iron tablet consumption variables. Results: There is a significant impact of knowledge about anemia (p = 0.000), date consumption (p = 0.0094), and iron tablet intake (p = 0.0073) on hemoglobin levels among prospective pregnant women. Conclusion: Knowledge about anemia influences hemoglobin levels, and there is an increase in hemoglobin levels after consuming dates and iron tablets among prospective pregnant women at the Klaten Selatan Community Health Center. Keywords: Knowledge, Dates, Iron Tablets, Hemoglobin Levels

Kegiatan pengabdian masyarakat ini dilakukan di Pesantren Al Fatih di Desa Klampar Kecamatan Proppo, Kabupaten Pamekasan Madura. Tujuan kegiatan pengabdian kepada masyarakat ini adalah untuk meningkatkan kualitas pengelolaan usaha peternakan sapi pedaging, dan untuk meningkatkan peran dan fungsi pondok pesantren sebagai pendidikan non formal guna mendukung ketahanan pangan serta penguatan penerapan biosecurity dalam upaya pencegahan PMK. Metode yang digunakan dalam kegiatan ini melalui observasi lokasi, diskusi dan wawancara, Participatory Rural, Participatory Technology Development, Educative, Forum Group Discussion (FGD). Hasil kegiatan menunjukkan bahwa peserta sangat antusias saat pelaksanaan. Edukasi yang diberikan pada peserta memberikan peningkatan pengetahuan tentang biosecurity yang signifikan, dari nilai pre-test 68.86 point, sedangkan nilai post-test 78.16 point, sehingga terjadi peningkatan 14%. Pengetahuan yang diaedukasikan meliputi; biosecurity PMK, sanitasi kandang, biosecurity keluar masuk wilayah farm/peternakan dan isolasi sapi yang sakit. Kesimpulan dalam kaegiatan ini adalah, peserta mampu memahami tentang materi peningkatan kualitas ternak melalui biosafety dan biosecurity PMK dan menerapkan teknologi tepat guna tentang formulasi pakan, dan sanitasi kandang untuk pencegahan dan reinfeksi PMK.

Background and Aim: Egg yolk (EY) is commonly used as an extracellular cryoprotectant in semen diluents but has some negative effects. Therefore, this study aimed to assess the potential of lecithin derived from plants, such as soybeans, as an alternative extracellular cryoprotectant and to characterize liquid semen quality of Ongole crossbred bulls using a modified caudal epididymis plasma-3 [CEP-3 (m)] as a base diluent and aqueous soybean extract (ASE). Materials and Methods: A bull with progressive motility (PM) of fresh semen >70% was used. Two soybean extracts were also used, namely, ASE 1 and ASE 2, obtained by extraction procedures 1 and 2, respectively. The study was conducted using an experimental design with 11 treatments and ten replications, with diluents comprising different levels of ASE 1 and ASE 2, as well as a positive control with 10% EY. The parameters measured were motility (M) and its kinetic parameters, including PM, M, velocity curve linear, velocity straight linear, velocity average pathway, linearity, straightness, wobble, amplitude lateral head beat cross frequency, and hyperactivity using computer-assisted sperm analysis, viability, and spermatozoa abnormalities. Results: The CEP-3(m) diluent formula and ASE 1 at a 30% level maintained the PM of spermatozoa up to day 5 (40.7% ± 16.1%) of cold storage. Meanwhile, the CEP-3(m) diluent formula and ASE 2 could only maintain PM >40% until day 3 (42.1% ± 13.5%) of cold storage at a 30% level. The CEP-3(m) diluent and ASE 1 at a level of 25%–30% supported spermatozoa life (viability) up to day 5 with a value >80% (81.8 ± 3.5; 86.4 ± 2.6). The abnormality value of spermatozoa in various diluents during cold storage on days 0–5 was below 20%. Conclusion: Soybean extracts 1 and 2 can substitute EYs as extracellular cryoprotectants in modified CEP-3 basic diluents. Soybean extract 1 can support the life of spermatozoa up to day 5 but may cause the viscosity and movement of spermatozoa to be hyperactive. Soybean extract 2 can support the life of spermatozoa up to the 3rd day of cold storage and produces progressive (non-rotating) movement patterns. Further, research is recommended with higher levels of extract 2.

Abstract The purpose of this study was to evaluate the effect of environmental factors, namely seasonal changes and nutritional factors on the adaptive abilities shown by the metabolic response of Madura cattle. The research was conducted in Pademawu District, Pamekasan Regency, East Java from February to June 2022. The research material included 45 Madura cattle which included differences in sex and age. Metabolic response was analyzed descriptively. The results of the study obtained blood urea nitrogen (BUN) concentrations in male and female Madura cattle with different ages, including; 20.96 ± 6.79; 22.04 ± 5.12; 18.63 ± 1.94; 15.86 ± 2.72; 27.42 ± 14.5; 21.40 ± 8.2. Triglyceride concentrations in Madura cattle successively at different sexes and ages, namely; 11.00 ± 3.53; 21.60 ± 7.4; 11.00 ± 1.94; 12.00 ± 2.72; 9.20 ± 5.65; 11.00 ± 2.40. Glucose concentrations in male and female Madura sai with different ages, namely; 83.00 ± 16.90; 102.40 ± 13.90; 97.60 ± 12.60; 110.00 ± 13.3; 95.00 ± 8.4; 54.80 ± 8.7. Blood protein concentrations respectively; 7.70 ± 1.28; 10.30 ± 2.09; 10.14 ± 2.48; 9.16 ± 1.73; 9.56 ± 2.01; 6.16 ± 1.55. In general, the metabolic response of male and female Madura cattle at different age levels in the wet season is within the normal range. This shows that it can adapt well. Keywords: Madura cattle; Metabolic; Response; Season Abstrak Tujuan penelitian ini adalah mengevaluasi respon metabolik sapi Madura pada musim basah. Materi penelitian meliputi 45 ekor sapi Madura yang meliputi perbedaan jenis kelamin dan umur. Respon metabolic dianalisis secara deskriptif, hasil penelitian didapatkan konsentrasi Blood urea nitrogen (BUN) pada sapi Madura jantan dan betina dengan umur berbeda antara lain: 20,96 ± 6,79; 22,04 ± 5,12; 18,63 ± 1,94; 15,86 ± 2,72; 27,42 ± 14,5; 21,40 ± 8,2. Konsentrasi trigliseride pada sapi Madura berturut-turut pada jenis kelamin dan umur yang berbeda yaitu; 11,00 ± 3,53; 21,60 ± 7,4; 11,00 ± 1,94; 12,00 ± 2,72; 9,20 ± 5,65; 11,00 ± 2,40. Konsentrasi Glukosa pada sapi Madura jantan dan betina dengan umur berbeda yaitu: 83,00 ± 16,90; 102,40 ± 13,90; 97,60 ± 12,60; 110,00 ± 13,3; 95,00 ± 8,4; 54,80 ± 8,7. Konsentrasi protein darah berturut-turut: 7,70 ± 1,28; 10,30 ± 2,09; 10,14 ± 2,48; 9,16 ± 1,73; 9,56 ± 2,01; 6,16 ± 1,55. Respon metabolic sapi Madura jantan maupun betina pada tingkatan umur yang berbeda di musim basah berada dalam kisaran normal. Kata kunci: Metabolik; Musim; Respon; Sapi Madura

Kambing merupakan sumber daging dan susu di banyak negara berkembang, karena kemampuannya untuk bertahan hidup dan tumbuh di bawah lingkungan iklim yang keras dibandingkan ruminansia lainnya. Fenomena ini memudahkan beternak kambing tanpa fasilitas yang canggih dan kebutuhan nutrisinya dapat dipenuhi dari berbagai jenis pakan atau padang penggembalaan yang tersedia. Tujuan utama dari penelitian ini adalah untuk mengevaluasi pengaruh penambahan biji kurma dalam pakan konsentrat terhadap rata-rata pertambahan bobot badan harian dan status gizi kambing Peranakan Etawah. Dua puluh ekor kambing dipilih secara acak dan didistribusikan ke dalam empat perlakuan masing-masing 5 ekor kambing. Kambing diberi pakan hijauan 60% Pennisetum purpureum dan 40% pakan konsentrat yang mengandung 0; 7.5; 15 dan 22,5% tepung biji kurma. Terdapat pengaruh yang nyata (P

This study aimed to determine the relationship between estrous characteristics with the success of AI in Madura cattle. A total of 50 female Madura cattle were used in this research. The estrus quality was observed, including vulva temperature, vulva swelling, vulva color, cervical pH, and cervical mucus. The parameters were NRR-1, NRR-2, Pregnancy Rate, and conception rate. The results showed that Madura cattle with the quality of estrus in the uneven red vulva color category showed better results than red vulva color at NRR-1, NRR-2, CR, and PR values, 100%, 93,33%, 93,33%, and 93,33% respectively. Abundant cervical mucus showed better results than the moderate mucus on the NRR-1, NRR-2, CR, and PR values, which were 100%, 87,5%, 75%, and 75%, respectively. Then a vulva temperature of 37,0-37,9ºC showed better results than a vulva temperature >38ºC in the NRR-1, CR, and PR values, namely 92,12%, 64,71%, and 70,59. Furthermore, the pH condition of cervical mucus with a pH value of 7 showed better results than cervical mucus with a pH of 8 at NRR-1, NRR-2, CR, and PR, 94,12%, 94,12%, 70,59%, and 76,47%. In the very swollen vulva category, the results were better than slightly swollen at the NRR-1 value of 100%. This study concludes that the characteristic uneven color of the vulva, the vulva temperature is 37,0-37,9, the abundant cervical mucus, cervical pH 7, and a very swollen vulva give a high percentage of the success of AI.

Jawa Timur merupakan provinsi dengan jumlah santri terbesar keempaat di Indonesia. Warisan budaya nyantri masih sangat lekat di masyarakat sehingga pertumbuhan pesantren senantiasa meningkat setiap tahunnya. Oleh sebabnya, pondok pesantren memiliki potensi sebagai agen pemberdayaan masyaraat. PP Nurul Islam (NURIS) merupakan pesantren yang telah eksis sejak 1969. Namun, pengembangan softskill santri sampai saat ini masih terbatas karena kendala finansial dan minimnya sumber daya. Tujuan pengabdian ini adalah meningkatkan keterampilan santri melalui pelatihan dan pengembangan produk usaha berbasis hidroponik. Adapun metode pengabdian antara lain: (1) Focus Group Discussion (FGD) dan sosialisai kegiatan; (2) workshop, praktik, dan pendampingan hidroponik; dan (3) evaluasi kegiatan. Rangkaian kegiatan terlaksana selama 3 bulan, diawali kegiatan pembibitan sayur dan pembuatan instalasi hidroponik 120 lubang. Selanjutnya workshop materi kewirausahaan dan hidroponik, serta pendampingan dan hilirisasi produk. Evaluasi kegiatan berdasarkan kuisioner. Panen pertama sercapai setelah 2 bulan HST dengan keuntungan bersih mencapai Rp. 185.000. Keberhasilan kegiatan tercapai berdasarkan indikator 100% kehadiran, peningkatan wawasan siswa berdasarkan skor pretest-posttest serta penilaian kebermanfaatan, kemenarikan, dan kejelasan program.

ABSTRACT. Penelitian ini bertujuan untuk mengevaluasi pejatan sapi Madura dengan menggunakan metode pendugaan nilai pemuliaan berdasarkan bobot badan dan ukuran tubuh. Pengambilan sampel dilakukan secara Purposive sampling dengan menggunakan data recording keturunan 7 pejantan sapi (684, Adikara/160726, 685, Kelesap/160725, Muntahai/16011001, Banteng/934, 386) untuk bobot badan (BB), tinggi badan (TB), panjang badan (PB) dan lingkar dada (LD) kelahiran tahun 2014-2020. Analisis data dengan koreksi umur 1 tahun ke 365 hari, nilai heritabilitas, nilai pemuliaan, dan korelasi rangking nilai pemuliaan. Hasil analisis nilai heritabilitas bobot badan dan ukuran tubuh sapi Madura umur 1 tahun tergolong kategori sedang hingga tinggi yaitu 0,57 (BB), 0,48 (TB), 0,83 (PB), dan 0,61 (LD). Sebanyak 42,85% bobot badan dan panjang badan pejantan sapi memiliki nilai pemuliaan positif, sedangkan pada tinggi badan dan lingkar dada memiliki nilai pemuliaan positif sebesar 28,57%. Korelasi rangking nilai pemuliaan bobot badan dengan ukuran tubuh sapi Madura umur 1 tahun tergolong kategori tinggi yaitu 0,86 (BB-TB), 0,68 (BB-PB) dan 0,86 (BB- LD). Kesimpulan dari penelitian ini yaitu pejantan sapi di UPT Pembibitan dan Kesehatan Hewan Provinsi Jawa Timur yang memiliki nilai pemuliaan positif pada bobot badan dan ukuran tubuh adalah pejantan Ke lesap/160725 dan Adikara/160726 sehingga pejantan tersebut dapat digunakan sebagai tetua dalam program seleksi untuk meningkatkan mutu genetik dan menghasilkan sapi unggul. (Evaluation of sire breeding values on Madura cattle based on body weight and body measurement of their offspring at one-year-age) ABSTRAK. This study aimed to evaluate Madura cattle by using the method of estimating breeding value based on body weight and body measurement. The sampling was carried out by Purposive sampling using data recording offspring the 7 cattle (684, Adikara/160726, 685, Kelesap/160725, Muntahai/16011001, Banteng/934, 386) for Body Weight (BW), Withers Height (WH), Body Length (BL), and Chest Girth (CG) of born in 2014-2020. The data were analyzed by correction of 1 year to 365 days of age, heritability value, breeding value, and correlation ranking of breeding values. The results of the analysis of heritability values of body weight and body measurement of Madura cattle at 1 year of age rating on medium to high category were 0,57 (BW), 0,48 (WH), 0,83 (BL), and 0,61 (CG). A total of 42,85% body weight and body length of cattle at 1 year age have a positive value, while the body height and chest girth has a positive breeding value of 28,57%. The correlation between body weight and body measurement at 1-year-age Madura cattle classified as high category was 0,86 (BW-WH), 0,68 (BW-BL) and 0,86 (BW-CG). The conclusion this study the cattle at the UPT Breeding and Animal Health of East Java Province which has positive values on body weight and body measurement are the Ke lesap/160725 and Adikara/160726 males, while sires can be used as elders in the selection program to improve genetic quality and to produce superior cattle.

ABSTRACT. Knowledge of farmers about estrus detection is one of the factors that affect fixed-time artificial insemination (AI). This study aims to evaluate the success rate of AI using Y sexing frozen semen based on the estrus character of cattle. Forty-five Limousin Crossed Cows involved in this research with a Body Condition Score of 3-5 (1-9 scale), 1.8-7 years old. Y sexing frozen semen is produced by the Singosari Center for Artificial Insemination using Percoll's Gradient Density Centrifugation method. Observation of estrus character was done before insemination with the deep insemination technique of AI. Artificial insemination was carried out using double doses at the 2nd and 8th hours after estrus. Rheinbio vitamins as BioATP+ are injected after AI. The results showed that the Conception Rate and Pregnancy Rate based on the estrus character was higher as indicated by the red colour of the vulva was 17.78% and 22.22%; very swollen vulva was 20% and 22.22%; abundant cervical mucus was 15.56% and 17.78%, and vaginal temperatures ranging from 38.0-38.5oC was 13.33% and 20%. In conclusion, the low percentage of pregnancy in this study was caused by various factors, mainly the genetic quality, early embryonic efficacy, maintenance management especially feed, and there were cows that experienced reproductive disorders. (Evaluasi keberhasilan inseminasi buatan menggunakan semen beku sexing berdasarkan karakter estrus yang berbeda) ABSTRAK. Pengetahuan peternak mengenai deteksi estrus merupakan salah satu faktor yang berpengaruh terhadap ketepatan waktu inseminasi. Penelitian ini bertujuan untuk mengevaluasi tingkat keberhasilan IB menggunakan semen sexing Y berdasarkan karakter estrus ternak. Akseptor yang digunakan pada penelitian ini berjumlah 45 ekor Sapi Persilangan Limousin dengan BCS 3-5 dan umur berkisar antara 1,8-7 tahun. Semen beku sexing Y merupakan hasil produksi Balai Besar Inseminasi Buatan (BBIB) Singosari menggunakan metode Sentrifugasi Densitas Gradien Percoll (SGDP). Pengamatan karakter estrus dilakukan sebelum inseminasi. Inseminasi Buatan dilakukan dengan menggunakan double dosis pada jam ke-2 dan jam ke-8 dengan teknik deep insemination. Vitamin Rheinbio sebagai BioATP+ diinjeksikan setelah IB. Hasil penelitian menunjukkan bahwa keberhasilan kebuntingan ditinjau dari nilai conception rate (CR) dan pregnancy rate (PR) berdasarkan karakter estrus ternak lebih tinggi ditunjukkan pada kondisi warna vulva merah merata yaitu 17,78% dan 22,22%; vulva yang sangat bengkak yaitu 20% dan 22,22%; banyak mengeluarkan lendir servik yaitu 15,56% dan 17,78%, serta suhu vagina yang berkisar antara 38,0-38,5oC yaitu 13,33% dan 20%. Persentase kebuntingan yang rendah pada penelitian ini disebabkan oleh berbagai faktor, utamanya kualitas genetik ternak, kematian embrio dini, manajemen pemeliharaan khususnya pakan, dan terdapat ternak yang mengalami gangguan reproduksi.

ABSTRACT. Penelitian ini bertujuan mengevaluasi keberhasilan IB menggunakan metode dosis tunggal dan ganda pada sapi Madura. Penelitian menggunakan 25 ekor dengan metode IB dosis tunggal dan 25 ekor dengan metode IB dosis ganda. IB menggunakan semen beku sapi Madura yang diproduksi oleh Balai Besar Inseminasi Buatan (BBIB) Singosari, Malang. Teknik deposisi semen adalah 4+ (deep insemination) yaitu pada posisi cornua uteri selanjutnya dilakukan IB dosis tunggal (jam ke-8 setelah menunjukkan tanda-tanda berahi) dan IB dosis ganda (jam ke-2 dan ke-8 setelah menunjukkan tanda-tanda berahi). Akseptor diinjeksi dengan Bio ATP+ merek “Rheinbio” 10 ml per ekor dan pemberian pakan konsentrat merek “Novo NC62” 1 kg per ekor per hari selama tujuh hari setelah IB. Variabel penelitian meliputi Non Return Rate (NRR), Conception Rate (CR), dan Service Per Conception (S/C). Hasil penelitian menunjukkan bahwa IB dosis tunggal menghasilkan nilai NRR 1 sebesar 92%, NRR 2 sebesar 80%, CR sebesar 60%, dan S/C sebesar 1,71. Sedangkan pada IB dosis ganda menghasilkan nilai NRR 1 sebesar 92%, NRR 2 sebesar 84%, CR sebesar 68%, dan S/C sebesar 1,53. Kesimpulan penelitian adalah nilai NRR, CR, dan S/C pada sapi Madura dengan IB dosis ganda lebih baik daripada dengan IB dosis tunggal, dan IB dosis ganda dapat meningkatkan conception rate pada sapi Madura. (The success different of artificial insemination using single and double dosage method of Madura cows) ABSTRAK. This study aims to evaluate the success of AI using methods single and double dosage plus in Madura cows. The study used 25 Madura cow using the AI method single dosage and 25 Madura cow using the AI method double dosage. AI uses frozen semen from Madura cows produced by the Singosari Center for Artificial Insemination. The semen deposition technique is 4+ (deep insemination) in the cornua uteri position then AI implemented with a single dosage (8th hours after showing signs of estrus) and double dosage (2nd and 8th hours after showing signs of estrus). The acceptors were injected with 10 ml of "Rheinbio'' brand Bio ATP+ per cow and 1 kg of concentrate "Novo NC62" brand per cow per day was given for seven days after IB. Research variables include Non Return Rate (NRR), Conception Rate (CR), and Service Per Conception (S/C). The results showed that AI of single dosage had value NRR 1 of 92%, NRR 2 of 80%, CR of 60%, and S/C of 1.71. While AI of double dosage had value NRR 1 of 92%, NRR 2 of 84%, CR of 68%, and S/C of 1.53. In conclusion, the value of NRR, CR, and S/C in Madura cows with AI double dosage was better than AI single dosage, and AI double dosage could increase the conception rate in Madura cows.

This study aimed to investigated the morphometric characteristics of Pote goats, a local Indonesian goat breed found in Bangkalan Regency, East Java Province. A total of 139 respondents participated in the study, providing 622 female and male Pote goats aged over three months as research materials. This study employed a descriptive research method, including surveys and interviews, to retrieve data using purposive sampling technique. Quantitative data, such as body weight and body size, were analyzed descriptively using the average and standard deviation. Qualitative data, including body color, facial shape/profile, horns, beard, papillae, rewos fur, and udder shape, were analyzed using phenotypic frequency. The findings of this study revealed that Pote goats in Bangkalan are a small type of local Indonesian goat based on the body weight of an adult female and a large type based on shoulder height. Body weight and body size were found to increase with age. Furthermore, the dominant qualitative characteristics of Pote goats in Bangkalan include white color on the body, convex facial profile, drooping and widened ears with relatively short size, straight right and left horns, mane ruff, no papillae, and jug-shaped udder.

Heat shock proteins (HSPs) associated with stress reactions play an important role in cell survival by activating numerous regulatory proteins and inducing black apoptosis. This study aimed to identify the potential SNPs of HSP70 gene in Kacang goats in Indonesia. Forty-three Kacang goats were selected from Sidoarjo and Tulungagung districts. The DNA isolated from blood samples was successfully amplified using the polymerase chain reaction (PCR) method with a pair of primers. The PCR products were sequenced in the coding region. The sequences were successfully aligned to determine the potential SNPs. A novel SNP (c.1311C>T) was found in this study. This SNP was categorized as a synonymous mutation. The insertion-deletion (in-del) mutation was also observed at the 1151st and 1161st nucleotide positions. Based on these mutations, four haplotypes were constructed where haplotype 1 had the highest frequency in Sidoarjo. Indonesian goats had no close relation with Iraqi goats according to Neighbor-Joing with Kimura’s 2-parameter approach. Haplotypes 1 and 2 in Indonesian goats had three different bases with all haplotypes in Iraqi goats in Median Joining Network. The study concluded that a novel SNP of HSP70 gene was identified in Kacang goats.

The objective of this research was to examine the population structure and production of Madura cows. Indonesian local Madura cows are originally from Madura island. Its development is constantly improved to sustain the existence and production of Madura cows as the main contributor to beef. The research was conducted in Lenteng sub-district, Sumenep district, East Java province from August to September 2023. The research method used was the survey method and sampling was done by accidental sampling. Madura cows data used were 263 heads. Data analysis using descriptive. The results showed that the population structure of Madura cows was 9.89% male calves and 13.69% females, 7.22% young males and 28.52% females, 0% bull and 41% cows. The number of cows to the population was 40.68%, the number of births to the population was 13.69%, the number of mortalities to the population was 2.28%, the Natural Increase was 11.41% with Calf Crop was 29.51%. The results indicated that the production of Madura cows is still low. It is suggested to procure selected males for artificial insemination or natural mating. Based on the population structure there is a rarity of males.

The purpose of this research was to figure out the population structure and productivity of Peranakan Ongole cattle in the breeding source area. The research was conducted from September 2022 to August 2023 in the breeding source area for Peranakan Ongole cattle, Napis village, Tambakrejo sub-district, Bojonegoro district, East Java province. Data was obtained by recording for 1 year on 1,015 heads Peranakan Ongole cattle. Research variables include population structure and cattle productivity. The results of population structure research showed that the number of male calves was 10.34%, female calves 8.13%, bulls 17.61%, and cows 63.93%. Peranakan Ongole cattle calf crop was 55.01%, calf births to the population were 37.04% with the number of male calves born at 53.46% and the number of female calves born at 46.54%. The percentage of cows to the population was 63.94%. The percentage of mortality to the population was 2.27%. Total livestock income to the population was 12.12% and total livestock output to the population was 31.13%. The natural increase in Peranakan Ongole cattle was 34.78%. The research results showed that the Calf crop value is quite good. To increase the percentage of calf crops, it is necessary to limit and select the production of prospective and productive heifers and cows. Prospective heifers and cows who are productive and have good potential must be preserved to increase the number of births and produce good-quality calves.

This investigation aimed to identify the production and composition of crops as feed, classify the types of integrated farming in different locations, and compare dairy goats based on their morphometric characteristics. The data used were collected from July to November, 2021 in the Malang, Lumajang, and Bangkalan regencies of Indonesia, via a survey and direct observations. Descriptive, multivariate, and variance analyses were adopted using RStudio to analyze the collected data. In Malang and Lumajang, farmers adopted agroforestry, alley cropping, and cover crops as the main types of integrated farming. The integrated farming adopted by the farmers in Malang, Bangkalan, and Lumajang helped to explain the differences in forage crops. Calliandra calothyrsus, Indigofera zollingeriana, and Thitonia diversifolia are non-grasses having high levels of average production. In Lumajang, farmers provided only a few combinations with Calliandra which was the most intensively grown crop. In Malang, six crops were combined for the dairy goat feed. Pennisetum purpureum was the crop most intensely grown to feed the PE (Etawa crossbred). In Bangkalan, Pote-Arosbaya goats were fed a combination of eight crops. Moringa oleifera, Bambusa sp., Artocarpus heterophyllus, and natural grasses were the most popular feed crops. In Malang and Lumajang, the PE Singosari and Senduro goats had a high degree of similarity based on their morphometric characteristics, respectively.

Molecular information using gene variants could support livestock selection to improve livestock productivity. This study aimed to identify the variants in part of the MSTN gene (part of the third exon coding region), a gene known for its significant effect on muscle growth. DNA was isolated from 95 Madura cattle blood samples collected in Pamekasan, Madura. PCR was used to amplify to MSTN region in part of the third exon, and one-way sequencing was conducted to obtain the sequence from the amplified product. Six SNPs were identified. Four SNPs were novel mutations, and the other two SNPs were recorded in the Ensembl database (rs466598800 and rs211583837). Among the identified novel mutations, one mutation in c.1301G/T caused a stop codon for the TT genotype cattle. However, no cattle carried the TT genotype. Three other mutations in c.1324, c.1373, and c.1399 were missense mutations that change the amino acids Q311L, A318P, and L335B, respectively. Most of the cattle had heterozygote genotypes for all the identified SNPs. It could also be noted that there were sequence differences in the part of the third exon identified between Madura cattle and Zebu cattle (by Tantia et al., 2006).

This study obtained morphometric information on Galekan cattle and compared phenotypic characteristics of Galekan cattle with local breed based on previous studies. This research was a case study with purposive sampling using the snowball sampling technique. The material used was 60 cattle of Galekan cattle which were kept in the Technical Unit (UPT) at Dongko and Panggul Sub Districts. The variables observed included qualitative characteristics and quantitative characteristics of morphometric components and body weight. T2-Hotelling test, descriptive statistics, and principal component analysis are used for quantitative data. The results showed that the qualitative characteristics of Galekan cattle were dominated by light brown body color with smear body color border, black backline, black vulva, black tail tip, face color as same as dominant body color, black muzzle, black upper and lower lips, smear white eye area, dark eye circles, black eyelashes, and black ear lines. The characteristics of the body shape of Galekan cattle have upward horns, no hump, and thin dewlap. The results of the T2-Hotelling analysis in UPT showed a significant difference (p

The study aimed to evaluate the production performance of male Limousine Crossbreed cattle with different feed levels with the addition of RD flour. It used nine male Limousine crossbreed cattle, aged 0,05) and on Daily gain, Average daily gain, and IOFC (P

Background and Aim: Madura cattle is local cattle in Indonesia. This cattle hasphenotypic variations in growth traits. This study aimed to identify leptin (LEP) gene polymorphism exon 2 associated with body measurements in Madura cattle. Materials and Methods: We recorded body weight (BW) and body measurements of 51 Madura cattle aged 1-4 years in Waru District, Madura. The LEP gene genotyping was conducted using the polymerase chain reaction-restricted fragment length polymorphism method with AciI restriction enzyme. Results: A 267 bp DNA fragment of the LEP gene was successfully amplified using a pair of primers. This study revealed three genotypes (TT, TC, and CC) and two alleles (T and C). The frequencies of TT, TC, and CC genotypes were 0.275, 0.45, and 0.275, respectively, whereas the frequencies of T and C alleles were 0.500 and 0.500, respectively. The c.73T>C mutation was significantly associated with BW, body length (BL), and chest girth (CG) (pC) exon 2 was polymorphic and associated with body measurements, especially BW, BL, and CG.

Latar belakang: Brain-derived neurotrophic factor (BDNF) merupakan neurotrophin yang disekresi oleh dendrit. BDNF berperan pada diferensiasi, maturasi dan menjaga survival neuron. BDNF juga berperan penting pada neuroplastisitas, learning and memory. Ekspresi BDNF menurun pada kondisi stres. Propolis mengandung chyrisin yang memiliki efek antioksidan dan neuroprotektan. Penelitian ini ingin melihat efek propolis terhadap ekspresi BDNF pada hippocampus tikus yang diinduksi stres. Tujuan: untuk mengetahui pengaruh pemberian propolis terhadap ekspresi BDNF di hippocampus pada tikus yang diinduksi stres. Metode: Penelitian eksperimental dengan menggunakan rancangan posttest only control group design. Subjek penelitian ini tikus Rattus norvegicus jantan, berumur 4 bulan dengan berat badan 200-300 gram dari galur Spraque-Dawley. Tikus dibagi secara random menjadi 5 kelompok, masing-masing kelompok terdiri dari 5 ekor tikus. Kelompok N tidak mendapat perlakuan. Kelompok K mendapat perlakuan stres, kelompok P1, P2 dan P3 mendapat perlakuan stres dan pemberian propolis dosis 100, 150 dan 200 mg/kgbb/hari. Perlakuan stres menggunakan model stres isolasi sosial. Pemberian propolis secara oral menggunakan sonde. Perlakuan stres dan pemberian propolis selama 14 hari. Hari ke-15 dilakukan terminasi dan pengambilan jaringan otak. Selanjutnya dilakuakan pembuatan sediaan histologis dengan pewarnaan imunohistokimia menggunakan antibodi anti BDNF. Pengamatan menggunakan mikroskop cahaya dengan perbesaran 1000 kali pada area CA1 hippocampus. Hasil: Terdapat perbedaan signifikan ekspresi BDNF di area CA1 hippocampus pada semua kelompok (p. 0.000). Ekspresi BDNF tertinggi pada kelompok P3. Ekspresi terendah pada kelompok K. Kesimpulan: Terdapat pengaruh pemberian propolis terhadap ekspresi BDNF pada hippocampus tikus yang diinduksi stres. Dosis propolis 100,150 dan 200mg/kgbb meningkatkan ekspresi BDNF.

The objective of this research was to evaluate the breeding value based on body weight and body measurement at one day of age in Madura cattle on the Technical Implementation Unit of Animal Breeding and Health East Java. Recording data used were withered height (WH), body length (BL), chest girth (CG), and birth weight (BW) that birth from 2014 until 2020. Analyzed variables consist of heritability, breeding value, and spearman correlation. The analysis result showed heritability for the performance of 1 day of age in Madura cattle categorized as a medium until high (0.33 – 0.64). Breeding value from 10 bull based on BW was 60% categorized positive. Meanwhile, breeding value based on WH, BL, CG was 40% categorized positive. The Spearman correlation of BW with WH and CG had a high correlation (0.73 and 0.78), while BW and BL had a low correlation (0.22). This research concluded that the highest breeding value of body weight and body measurement of Madura cattle at 1 day was sire Montahai/1661100. The genetic potency of Madura cattle should be improved by applying for a consistent breeding program with a sound recording and selection program.

This research aims to determine the quantitative characteristics of Brahman cross heifer cattle's carcass and wholesale cut at different age levels. The number of heifer cattle taken as samples was 104 heifer. The research method used is a case study with the selection of locations by purpose sampling. Direct observation is used to obtain information on age, slaughter weight, carcass weight, wholesale cut weight, and parts thereof (primary, secondary, and manufacturing cut). The data obtained were then analyzed by analysis of variance (ANOVA). If there was a significant difference, it is followed by a further test of the Least Significant Difference (LSD). The results showed that the age levels in slaughter had a significant effect (P

A research group of Animal Science Faculty of Brawijaya University introduced and implemented Frozen sexed semen Artificial Insemination to 120 beef cattle farmers in Palang Village, Tuban Regency, East Java, from 2017 to 2019. The result of the program reported that the success rate of the technology is quite high, with the birth rate of male claves up to 80%. However, farmers’ adoption rate of the technology was relatively low. This research aims to evaluate factors that affected the adoption rate including the technology, characteristics of respondents, and the extension agents. Primary data were collected through a census of 120 farmers involved in the program and in-depth interviews with stakeholders. Secondary data were collected through related documents including reports of the programs, Statistics, and other related documents. This research utilized Quantitative and qualitative analyses. Data of the study indicated that respondents have neutral perceptions of the Frozen Semen Sexing Artificial Insemination. Although male calves relatively have a higher price, more than 50% of respondents did not expect specific sexing for the calves. In-depth interview data revealed that the success of the Artificial insemination technology is more valuable for the farmers rather than the sexing. Characteristics of respondents and the extension agents played significant roles in the adoption rate of the Frozen Sexed Semen Artificial Insemination technology adoption in Palang Village, Tuban Regency, East Java.

Tujuan penelitian ini adalah untuk mengidentifikasi karakteristik sapi Madura betina tipe taccek berbagai umur. Penelitian dilakukan di wilayah sumber bibit sapi Madura yang berada di Kecamatan Pasean dan Kecamatan Batumarmar, Kabupaten Pamekasan, Provinsi Jawa Timur. Penelitian dimulai tanggal 1 Desember sampai 30 Desember 2019. Materi yang digunakan dalam penelitian adalah sapi Madura betina tipe taccek berbagai umur berdasarkan PI (Permanent incicivi) sebanyak 110 ekor dengan rincian PI0 22 ekor, PI2 10 ekor, PI4 25 ekor, PI6 19 ekor dan PI8 34 ekor. Metode penelitian yang digunakan adalah survei di lapangan dengan pengambilan datasecara purposive sampling. Data statistik vitaldihitung nilai rataan dan standar deviasinya, selanjutnya dianalisis dengan menggunakan ANOVA satu arah (one way anova) dan dilanjutkan Uji Beda Nyata Terkecil (BNT) untuk membedakan 2 rataan. Data fenotip dianalisis secara deskriptif dan diamati proporsi fenotipnya. Hasil penelitian disimpulkan bahwa seiring bertambahnya umur sapi taccek maka panjang kepala, lebar kepala, bobot badan, panjang badan dan lingkar dada ukuranya bertambah. Ukuran tinggi gumba dan tinggi pinggul juga bertambah mulai PI0, PI2, PI4, sampaiPI6,namun mengalami penurunan pada PI8. Sapi taccek memiliki ciri fenotip yang spesifik yaitu bentuk mata sipit dengan garis lingkar mata hitam, bentuk telinga kecil, tanduk melengkung ke atas, berpunuk, mempunyai gelambir dan rambut ekor berwarna hitam.

ABSTRAK. Penelitian ini bertujuan mengevaluasi tingkat keberhasilan IB semen beku sexing Y. Penelitian menggunakan 39 sapi Peranakan Ongole dan 77 sapi Persilangan Limousin yang di IB secara double dosis. IB menggunakan semen beku spermatozoa Y dengan metode pemisahan Sentrifugasi Gradien Densitas Percoll (SGDP) sapi Limousin yang diproduksi oleh Balai Besar Inseminasi Buatan (BBIB) Singosari. Teknik deposisi semen adalah 4+ yaitu pada posisi cornua uteri selanjutnya dilakukan IB pada jam ke 2 dan ke 8. Untuk akseptor dilakukan penyuntikan BioATP+ dan pemberian pakan konsentrat 3 kg per hari selama 3 hari setelah IB. Variabel penelitian meliputi Non Return Rate (NRR), Service Per Conception (S/C), dan Conception Rate (CR). Hasil penelitian menunjukkan bahwa IB sapi Peranakan Ongole dan sapi Persilangan Limousin yaitu NRR1 sebesar 82,05% dan 89,61%, nilai NRR2 sebesar 76,92% dan 84,42%, nilai CR sebesar 58,97% dan 74,03%, serta nilai S/C sebesar 1,78% dan 1,46%. Kesimpulan penelitian adalah IB menggunakan semen beku hasil sexing Y sapi Limousin pada sapi persilangan Limousin memiliki tingkat keberhasilan yang lebih tinggi dibandingkan sapi Peranakan Ongole. (The success rate of artificial insemination using frozen sexing semen of different breed cow) ABSTRACT. This study aimed to evaluate the success rate of AI frozen semen sexing Y. The material in this study used 39 Filial Ongole and 77 Limousin crossbred cattle in AI in double dose. The spermatozoa used were Y frozen semen of Limousin bull with the Percoll Gradient Density Centrifugation (SGDP) separation method produced by the Singosari Center for Artificial Insemination. The semen deposition technique was 4+, which has the position of the cornua uteri (deep Insemination), then AI implemented with a double dose at the 2nd and 8th hours after estrous. The cow acceptor was injected with BioATP+ and feed by 3 kg concentrate per day for three days after AI. Research variables include Non-Return Rate (NRR), Service Per Conception (S/C), and Conception Rate (CR). The results showed that AI of Peranakan Ongol and Limousin crossbred cattle on NRR1, NRR2, CR values, and S/C values respectively was 82.05% and 89.61%; 76.92% and 84.42%; 58.97% and 74.03%; 1.78% and 1.46%. In conclusion, AI using frozen semen from sexing Y Limousin cattle in Limousin crossbred cows had a higher success rate than Filial Ongole cattle.

Ketamine and ketamine-xylazine are often used as anesthetic drugs in animal models of ischemia. However, their neuroprotective and neurotoxic effects in ischemic animal models that have undergone tBCCAO are still under debate. The protein p53 is a pro-apoptotic factor involved in the cellular mechanism of ischemia. The interaction between death-associated protein kinase 1 (DAPK 1) and p53 is fundamental in determining whether cells experience necrosis or apoptosis in an ischemic stroke. This study was purposed to identify the presence or absence of differences between the p53 expressions in the brains of tBCCAO-induced ischemic rat models after the administration of ketamine and ketamine-xylazine. It employed a post-test control group design with four groups of adult male Wistar rats as the subject: (1) sham group operated with ketamine, (2) sham group operated with ketamine-xylazine, (3) models of tBCCAO-induced ischemia with ketamine, and (4) models of tBCCAO-induced ischemia with ketamine-xylazine. Ketamine was administered at the dose of 75mg/kg BW, while xylazine was at 8 mg/kg BW. The expression of p53 in rat brains was assessed by semi-quantification, specifically IHC staining with anti-p53 antibodies. P53 expression appeared as brownish stains in the cytoplasm of forebrain pyramidal neurons, and in this study, it was measured using the Allred score. The ANOVA test yielded a p-value of >0.05, implying the absence of difference between the p53 expressions in the brains of tBCCAO-induced ischemic rat models receiving ketamine and ketamine-xylazine.

Pote goat is one of the local Indonesian goats from Bangkalan, Madura, which has not been clumped. Procurement of Pote goat breeds as germplasm in enriching genetic diversity can be done by genetic selection. This study aims to identify the GDF9 gene associated with litter size traits of Pote goats. The materials used in this study were 48 female goats that had given birth with a range of permanent incicivi (0,1,2,3,4), and with healthy conditions. This research method uses survey methods and observations in the laboratory. Sampling was done by purposive sampling. Pote goat blood samples were taken from a smallholder farm in Soket Laok Village, Bangkalan Regency, Madura, East Java. GDF9 gene has a length of 490 bp. DNA fragments were amplified and genotyped using PCR-RFLP method using Msp1 restriction enzyme. This study resulted in two genotype types (AG, and GG), and two alleles (A and G). Genotype frequencies of AG, and GG were 0.40, and 0.60. The frequencies of alleles A and G are 0.40 and 0.80. The a.2912 A>G mutation was significant with the litter size. The results showed an association between GDF9 gene diversity found with litter size traits in Pote goats (p

This study aims to analyze customer satisfaction of goat kid and service quality attributes that are a priority to increase customer satisfaction. This research was conducted at Goat Production Farm of East Java Livestock Agency in Toyomarto Village, Singosari District, Malang, East Java, which is a producer of Etawa Crossbreed and Senduro goats. Respondents of this research were carried out using the total sampling method of buyers of goat kid from 2021 to 2023 with a total of 16 respondents. The research variables represent the elements of the marketing mix known as the 7P (product, price, place, promotion, process, physical evidence, and people) which are divided into 17 attributes. Primary data in this study were obtained through surveys and interviews assisted with instruments in the form of questionnaires. At the same time, the secondary data in this study were performance reports and literature. The results showed a customer satisfaction index of 74.02% in the good category. The main priority for performance improvement can be made to improve the genetic quality of parents in order to produce good quality offspring, completeness of sales supporting documents, adequacy of sales promotion information, after-sales service in the form of customer technical assistance. Furthermore, the mechanism determines the price of goat kid, the ease of the payment process through receipts, the availability of non-cash payments, and the existence of proof of payment accompanied by agency identity.

Tujuan dari penelitian ini adalah untuk mengetahui peningkatan conception rate melalui insmeinasi buatan dengan menggunakan semen sexing double dosis pada sapi persilangan ongole. Dalam penelitian ini 96 ekor sapi induk persilangan ongole yang terdiri atas 64 ekor sapi yang diinseminasi dengan semen beku dan 32 ekor sapi yang diinseminasi dengan semen sexing beku double dosis. Sapi yang digunakan dipilih secara acak dengan berdasarkan beberapa kriteria yaitu bebas dari gangguan reproduksi dan menunjukkan tanda-tanda estrus yang jelas. Tempat deposisi semen adalah 4+ atau cornua utery untuk meningkatkan peluang keberhasilan kebuntingan. Penelitian ini terdiri dari dua perlakuan yaitu inseminasi dengan semen beku non sexing (P1) dan semen sexing beku (P2). Parameter yang diamati adalah Non Return Rate (NRR), Service per Conception (S / C) dan Conception Rate (CR). Hasil penelitian menunjukkan bahwa NRR 1 dan NRR2 pada P1 adalah 90,63% dan 79,69%, sedangkan pada P2 masing-masing adalah 81,25% dan 78,12%. Nilai S / C pada P1 adalah 1,09 sedangkan pada P2 adalah 1,19. Sedangkan, nilai CR pada P1 dan P2 masing-masing adalah 43,75% dan 68,75. Dapat disimpulkan bahwa IB dengan semen sexing double dosis dapat meningkatkan nilai conception rate pada Sapi Persilangan Ongole.

The purpose of artificial insemination using sexing semen was to obtain the calf according to the expected sex. This study aimed to determine the success of artificial insemination in non-sexing and sexing of frozen semen. The materials used in this research were 77 non-sexing artificial insemination acceptor cows and 77 sexing artificial insemination acceptor cows with double dose treatment. The method used in this study was experimental field experiment with the research treatment: P0 = frozen non-sexing semen straw and P1 = frozen semen of spermatozoa Y by sexing method using Percoll Density Gradient Centrifugation (SGDP) produced by Balai Besar Inseminasi Buatan (BBIB) Singosari. The variables of this study were Non Return Rate (NRR), Service Per Conception (S/C) and Conception Rate (CR). The data obtained were analyzed descriptively. The results of the study in each non-sexing and sexing artificial insemination treatment were: the percentage of NRR 1 were 90.91% and 77.92%, followed by NRR 2 with 88.31% and 76.62%, the pregnancy percentage were 84.42% and 75.33%, and the CR values were 79.22% and 57.14%. The conclusion of this study was artificial insemination using frozen non-sexing semen had a higher success rate compared to artificial insemination using frozen sexing semen.

Kualitas spermatozoa pada semen beku sexing sangat penting dalam menunjang keberhasilan inseminasi buatan untuk mendapatkan pedat dengan jenis kelamin sesuai harapan. Tujuan penelitian ini bertujuan untuk mengetahui kualitas semen beku semen beku non sexing dan sexing secara makroskopis dan mikroskopis. Materi dalam penelitian menggunakan starw semen beku non sexing dan sexing dengan metode pemisahan Sentrifugasi Gradien Densitas Percoll (SGDP) sapi Limousin yang diproduksi oleh Balai Besar Inseminasi Buatan (BBIB) Singosari, Malang. Metode penelitian ini menggunakan eksperimental dengan percobaan labolatorium (laboratory experiment). Variabel penelitian meliputi keadaan umum meliputi tahun produksi straw, secara makroskopis meliputi pH semen beku dan secara mikroskopis meliputi persentase motilitas, viabilitas, abnormalitas, konsentrasi dan total spermatozoa motil. Data yang diperoleh diolah Microsoft Excel dengan analisis Ragam Analysis of variance (Anova) single factor dianalisis secara deskriptif. Hasil penelitian yaitu semen beku non sexing dan sexing meliputi keadaan umum diproduksi tahun 2012 dan 2018, kualitas secara makroskopis memiliki nilai pH sebesar 6,67 dan 6,60 dan kualitas secara mikroskopis memiliki persentase motilitas sebesar 36,00 % dan 31,40 %, viabilitas sebesar 81,70 % dan 75,89 %, abnormalitas sebesar 6,93 % dan 6,78 %, konsentrasi spermatozoa sebesar 31,67 juta/ mini straw dan 12,125 juta/ mini straw dan total spermatozoa motil sebesar 11,39 juta/mini straw dan 5,10125 juta/mini straw. Kesimpulan penelitian bahwa Kualitas post thawing semen beku non sexing lebih tinggi daripada semen beku sexing menggunakan metode SGDP baik secara makroskopis maupun mikroskopis.

Dates are a good source of nutrition for the body if consumed regularly both in the form of dried fruit, wet, as well as in the form of palm juice extracts, especially for pregnant and maternal mothers. Pregnant women who are going to give birth are in desperate need of drinks and foods that are rich in sugar, this is because of the many contractions of the uterine muscles when it comes to removing the baby, especially if it takes a long time. Dates contain potuchin hormone which functions to bind the uterus and muscles of the uterus so that it can help reduce postpartum bleeding. Besides, there is the hormone oxytocin which can help stimulate contractions in the muscles of the uterus so as to facilitate labor. The purpose of this study was to determine the effect of consumption of dates on bleeding, length of labor and type of labor. This type of research is a Pre experiment with a post-test Only Control Group Design research design. The population in this study were all pregnant women in the working area of South Klaten Public Health Center with estimated deliveries from July to September 2018. While the sample size was 60 samples consisting of 30 treatment group respondents and 30 control group respondents. The sampling technique used in this study was Quota sampling. Data analysis using Fisher Exact test and Mann-Whitney U test with p-value considered significant is p = 0.05. The results of the study of bleeding showed that there were no significant differences in the estimation of blood loss and during labor and type of delivery between the treatment group and the control group (p-value = 0.5). The results of the study about the length of labor showed that there was an effect of date consumption on the length of labor, with a value of p = 0,000

Coconut water extender is the solution to the difficulty and high cots of procurement of diluents. The objective of this study was to examine the effect of various formulations of coconut water extender on quality of PO bull sperm during storage in 4-5 oC. This study was carried out at Animal Reproduction Laboratory of Faculty of Animal Husbandry Brawijaya University and Laboratory of Beef Cattle Research Center Grati Pasuruan on March to April, 2018. The study method used laboratory experimental. The experimental design was Factorial Randomized Completely Block Design and the data were analyzed by Analyze of Variance. There are four treatments in this study (P0 = CEP-3 + 20 % of yolk, P1 = Coconut Water + 20% of yolk, P2 = Coconut Water + 20% of yolk + 0,4% white egg + fructose 1 mg/ml, dan P3 = Coconut Water + 20% of yolk + 0,4% white egg + fructose 2 mg/ml) and ten replications of each treatment. There were significant differences in sperm motility between CEP-3 extender and coconut water-based with various formula extenders during chilled storage at 2-5 oC. But the treatment between coconut water-based formulas did not show a significant difference. Coconut water extenders are able to maintain sperm motility up to day 5 with values above 57%.

Air kelapa merupakan bahan lokal yang murah dan dapat menjadi bahan baku alternatif dalam proses pembuatan pengencer semen karena mengandung beberapa zat yang dibutuhkan oleh spermatozoa seperti sumber energi berupa fruktosa. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan fruktosa dalam pengencer air kelapa hijau terhadap motilitas spermatozoa sapi PO (Peranakan Ongole). Penelitian ini menggunaan semen dari bangsa sapi Peranakan Ongole yang ditampung menggunakan metode vagina buatan. Metodologi yang digunakan adalah eksperimental laboratorium menggunakan rancangan acak kelompok (RAK). Penelitihan ini menggunakan 3 perlakukan, P1 = Pengencer air kelapa + kuning telur20%, P2 = Pengencer air kelapa + kuning telur 20% + putih telur 0,4%+ fruktosa 1 mg/ml. P3 = Pengencer air kelapa +kuning telur20% + putih telur 0,4% + fruktosa 2 mg/ml dengan 10 ulangan. Hasil memperlihatkan bahwa tidak terdapat perbedaan yang signifikan pada motilitas spermatozoa dan total spermatozoa motil antara formulasi pengencer air kelapa (P1, P2, P3) selama peyimpanan suhu 2-5 oC. Pengencer air kelapa baik dalam menjaga motilitas spermatozoa dan total spermatozoa motil hingga hari ke lima. Kata kunci: air kelapa, total motilitas motil, semen cair

Artificial Insemination (AI) is a technique that is widely used by farmers to improve the genetic quality of livestock. This method can be increased in value by using sexing semen that can regulate and produce the sex of the offspring as expected. The purpose of this study was to determine the success of AI by using frozen sexing semen on Ongole Crossbred cows in Malang Regency. The materials used were 98 Ongole crossbred cows in traditional farm of Malang Regency especially in Pakis, Tumpang, Jabung and Singosari Districts. The method used was a field experiment with two treatments: 49 cows were inseminated by using frozen sexing semen (P1) and 49 cows were inseminated by using non sexing semen (P0). The variables observed were the Non-Return Rate (NRR) value observedin 3 estrous cycle after AI and CR value based on pregnancy observation at 8 months of pregnancy. Data were analyzed descriptively and continued with a one-proportion z-test. The datashowed that the CR value on P1; P0 were 55.10% and 65, 31% respectively.furthemore, the NRR value on P1 from NRR1 to NRR3 were 87,76%, 73,47% and 67,35%, while on P0 95,92%, 83,67% and 75,51% respectively.It can be conclude thatCR and NRR value of sexing semenwas lower than non sexing semen, however sexing semen can be applied for certain purposes such as producing offspring as expected

The Artificial Insemination (AI)Â results in Beef cattle are expected to be male calf because they can increaseweight gain rapidly, so in this study AI was carried out by using sexing semen with Percoll density gradient centrifugation method containing Y sperm.This study was conducted to evaluate the success rate of AI using frozen sexing semen on Ongole Cross Breed. The study method used wasexperimental, comparing AI frozen sexing semen and frozen semen in 54 Ongole Cross Breed. The study variableswere Non Return Rate1 (NRR1), Non Return Rate2 (NRR2) and Conception Rate (CR).The study showed that the success rate of based on the value of NRR1 and NRR2AI using frozen sexing semen on Ongole Cross Breed is lower than un sexed frozen semen with the NRR1 value is 73,91%; 93,54% while the value of NRR2 was 69,56%; 83,87%. Based on CR value of frozen sexing semen higher than un sexed frozen semen showed CR valuewas 43,47%; 35,48%.

Penelitian ini bertujuan untuk mengetahui pengaruh pengencer CEP-3 kuning telur terhadap kualitas semen cair sapi Peranakan Ongole (PO) pada media simpan yang berbeda. Penelitian dilaksanakan di Laboratorium Lapang Fakultas Peternakan Universitas Brawijaya pada bulan Agustus sampai September 2017. Materi penelitian yang digunakan yaitu semen segar sapi Peranakan Ongole (PO) berumur 2 tahun ditampung menggunakan vagina buatan. Metode penelitian yang digunakan adalah eksperimental laboratorium dengan 5 perlakuan yaitu P0 (media simpan refrigerator suhu 5 oC), P1 (media simpan termos kosong suhu 28 oC), P2 (media simpan termos berisi es batu suhu 0 oC), P3 (media simpan termos berisi air es suhu 9 oC), dan P4 (media simpan termos berisi air sumur suhu 25 oC) masing-masing perlakuan menggunakan 10 ulangan apabila terdapat perbedaan yang nyata dilanjutkan dengan uji Jarak Duncan. Hasil analisis ragam pada penyimpanan hingga jam ke-8 menunjukkan berbeda sangat nyata (P 70 % dikemas didalam dan tanpa straw.

Studi ini bertujuan untuk mengevaluasi kecukupan nilai nutrisi untuk induk sapi potong di Desa Leran Wetan dan Leran Kulon, Kecamatan Palang, Kabupaten Tuban. Data diambil dengan metode survei menggunakan kuisioner kepada 15 responden di masing-masing desa. Sampel pakan diambil di masing-masing peternak responden kemudian dianalisis proksimat dan dilanjutkan uji kecernaan BK dan BO secara in vitro di Laboratorium Nutrisi dan Makanan Ternak, Fakultas Peternakan, Universitas Brawijaya. Analisis data dilakukan secara deskriptif kuantitatif. Hasil survei menunjukkan bahwa rata-rata kepemilikan induk sapi potong di masing-masing desa antara 2-3 ekor, bobot badan 300-350 kg/ekor, dengan tujuan pemeliharaan untuk menghasilkan pedet. Jenis pakan yang sering diberikan bervariasi meliputi rumput gajah, rumput lapang, limbah pertanian seperti jerami padi dan jerami jagung serta dedak padi sebagai pakan tambahan. Konsumsi BK pakan di Desa Leran Wetan rata-rata adalah 5,853 ± 1,663 kg/ekor/hari dan di Desa Leran Kulon adalah 5,943 ± 1,325 kg/ekor/hari sedangkan kebutuhan berdasarkan bobot badannya adalah 7,4 – 8,3 kg/ekor/hari. Ransum dengan pakan basal limbah pertanian di Desa Leran Kulon memiliki KcBK sebesar 27,844 ± 0,679% sedangkan ransum dengan pakan basal rumput memiliki nilai KcBK lebih tinggi yaitu 44,919 ± 1,800%. Nilai KcBK Ransum berbasis jerami di Desa Leran Wetan adalah 27,918 ± 0,692% dan yang berbasis rumput adalah sebesar 44,434 ± 2,026%. Nilai KcBO di Desa Leran Kulon pada ransum berbasis jerami dan rumput berturut-turut adalah 30,234 ± 46,412% dan 46,412 ± 1,778% sedangkan di Desa Leran Wetan berturut-turut adalah 33,689 ± 1,081% dan 52,989 ± 3,139%. Berdasarkan hasil studi tersebut, dapat disimpulkan bahwa pakan yang dikonsumsi oleh ternak masih belum memenuhi kebutuhannya. Penggunaan limbah pertanian dapat menurunkan nilai kecernaan ransum maka sebaiknya diganti dengan hijauan seperti leguminosa dan rumput. Pakan dengan kualitas yang baik akan meningkatkan performa reproduksi induk sapi potong.

Penelitian ini bertujuan untuk mengetahui karakteristik fenotip kerbau rawa (B. bubalis carabenesis). Penelitian ini dilaksanakan di Desa Guosobokerto Kecamatan Welahan Kabupaten Jepara. 211 kerbau lokal, terdiri dari 117 kerbau betina dan 91 kerbau jantan yang dikelompokkan berdasarkan jumlah pergantian gigi seri permanen yaitu PI0, P12, P14, P16dan P18. Data dianalisa dengan menggunakan annova way classification test. Hasil penelitian menunjukkan bahwa terdapat 3,79% bentuk tanduk melingkar kebawah yang tidak sesuai dengan SNI 7706.1.2011. Umur memberikan pengaruh yang sangat nyata (P

ABSTRAK. Sexing spermatozoa bertujuan untuk mengatur jenis kelamin sesuai harapan. Salah satu metode sexing adalah dengan menggunakan Sentrifugasi Gradien Densitas Percoll. Tujuan dari penelitian ini adalah untuk mengetahui berbagai parameter motilitas menggunakan Computer-assisted Sperm Analysis dan proporsi spermatozoa X dan Y menggunakan metode Sentrifugasi Gradien Densitas Percoll dengan volume awal yang berbeda. Penelitian dilakukan di Loka Penelitian Sapi Potong Grati, Pasuruan, pada bulan Januari sampai Maret 2020. Materi yang digunakan adalah Semen Sapi Peranakan Ongole berumur ±5 tahun dan bobot badan ±700 kg sebanyak 3 ekor, motilitas masa ≥ 2+ dan motilitas individu ≥ 70%. Metode yang digunakan adalah eksperimental menggunakan Rancangan Acak Kelompok (RAK) dengan 3 perlakuan volume saat sexing yaitu P1= 1 ml, P2= 1,5 ml dan P3= 2 ml dengan ulangan 11 kali. Ulangan juga berfungsi sebagai kelompok (block). Hasil penelitian menunjukkan bahwa setelah dilakukan sexing dan pendinginan hingga 5oC persentase motilitas adalah P1:71,02+10,08 %; P2: 79,63+8,65 % dan P3: 83,38+6,67 %, sedangkan motilitas progresif pada P1: 47,68+8,71%; P2: 59,61+7,26 % dan P3: 62,21+6,74%. Curvilinear Velocity (VCL) pada P1: 50,9±7,73 µm/s, P2: 55,2±5,03 µm/s dan P3: 53,2±5,97%. Straight-line Velocity (VSL) pada P1: 20,8±8,19 µm/s; P2: 21,6±6,02 µm/s; P3: 22,1±5,77 µm/s, sedangkan Average Path Velocity (VAP) pada P1: 30,2±7,82 µm/s; P2: 32,5±6,14 µm/s dan P3 : 31,5±6,18 µm/s. Linearity (LIN) pada P1: 40,2±12,26%;P2: 39,1±10,31%;P3: 39,8±7,25%. Straightness (STR) pada P1: 67,2±11,20%; P2: 65,7±10,06%;P3: 67,2±7,92% sedangkan Wobble (WOB) pada P1: 59±9,49%; P2: 58,8±8,63% dan P3: 59,1±7,74%. Kesimpulan dari penelitian adalah motilitas dan motilitas progresif spermatozoa pada sampel dengan volume awal 2 ml lebih baik dibandingkan dengan sampel dengan volume awal 1 ml dan 1,5 ml. (Characteristics of Sexing Spermatozoa Motility in Ongole Cattle with Different Initial Volume) ABSTRACT. Sperm sexing is a technique of sorting a specific type of sperm cell to fertilize the egg cell. One of the sexing methods that can be used for spermatozoa sexing is percoll gradient density centrifugation. This research aims to find out various motility parameters using Computer-assisted Sperm Analysis and the proportion of spermatozoa X and Y using density gradient centrifugation method percoll with different initial volume. The study was conducted at Grati Beef Cattle Research Station, Pasuruan, from January to March 2020. Materials of the study were Semen of 3 Filial Ongole Cattles, aged ± 5 years, with ± 700 kg body weight, mass motility ≥ 2+, and individual motility ≥ 70%. The method used was experimental using a Randomized Group Design with 3 treatment volume when sexing i.e. T0= 1 ml, T1= 1.5 ml dan T2= 2 ml with repetition 11 times. The repetition also functions as groups (blocks). The results showed that after sexing and cooling up to 5oC, the percentage of motility is T0:71.02+10.08 %; T1: 79.63+8,65 % and T2: 83.38+6.67 %. Progressive motility on T0: 47.68+8.71%; T1: 59.61+7.26 % and T2: 62.21+6.74%. Curvilinear Velocity (VCL) on T0: 50.9±7.73 µm/s, T1: 55.2±5.03 µm/s and T2: 53.2±5.97%. Straight-line Velocity (VSL) on T0: 20.8±8.19 µm/s; T1: 21.6±6.02 µm/s; T2: 22.1±5.77 µm/s. The Average Path Velocity (VAP) on T0: 30.2±7.82 µm/s; T1: 32.5±6.14 µm/s and T2 : 31.5±6.18 µm/s. Linearity (LIN) on T0: 40.2±12.26%; T1: 39.1±10.31%;T2: 39.8±7.25%. Straightness (STR) on T0: 67.2±11.20%; T1: 65.7±10.06%; T2: 67.2±7.92%. Wobble (WOB) on T0: 59±9.49%; T1: 58.8±8.63% and T2: 59.1±7.74%. The conclusion of the study is the motility and progressive motility of spermatozoa in samples with an initial volume of 2 ml is better than samples with an initial volume of 1 ml and 1.5 ml.

Among the various climate variables, heat stress has been reported to be the most detrimental factor to the economy of the livestock industry. Heat stress is one of the most stressful events in the life of livestock with harmful consequences for animal health, productivity and product quality. Heat shock proteins (HSPs), also known as molecular chaperons, are prominent stress markers. Heat shock proteins consist of highly conserved protein expressed at the time of stress, and play an important role in adaptation to the environmental stress. This review discusses the scientific evidence regarding the effects of heat stress and role of HSP during heat stress on Goats.

Increased reproduction is closely related to increased bull productivity. The high productivity of these bulls will increase the amount of straw. The productivity capability of Bali bull is essential in producing straw. High productivity will result in high straw production as well. So the research aims to determine the productivity of Bali bull at SNAIC in the last year. 31 Bali bulls aged 4 – 11 years were used in this study at SNAIC. Total 1.273 ejaculations collection from these during one year. The parameters used in this study include the number of successful holding of each bull, total productivity, and estimation of straw production in one year. From 31 Bali Bulls, the ranking was grouped into two based on values of sperm productivity. The two groups were analyzed using an unpaired T-test. The results showed that the pH, volume, individual motility, concentration, and abnormality were significantly different (P < 0.05) between the high and low groups. The percentage of successful shelters, total TSM, and the number of straws produced in one year in the high productivity group were higher than the low productivity group. High productivity groups are the best with high productivity potential and potential as a source of semen.

ABSTRAK. Pejantan untuk program inseminasi buatan adalah pejantan unggul yang diseleksi berdasarkan libido dan produksi spermatozoa. Penelitian bertujuan untuk menganalisa hubungan antara tingkah laku seksual dengan produksi spermatozoa sapi Brahman. Materi penelitian adalah 3 pejantan sapi Brahman yang ditampung menggunakan vagina buatan. Metode penelitian adalah observasional dengan mengamati waktu reaksi dan lama ejakulasi. Observasi laboratorium dengan mengamati volume, konsentrasi, dan motilitas individu untuk menghitung total spermatozoa (TS) dan total spermatozoa motil (TSM). Pengamatan dilakukan sebanyak 10 ulangan. Data dianalisa secara deskriptif serta hubungan antara tingkah laku seksual dan produksi spermatozoa dihitung dengan korelasi Pearson. Hasil penelitian menunjukkan terdapat variasi tingkah laku seksual dan produksi spermatozoa pada sapi Brahman. Individu Cipta mempunyai waktu reaksi dan lama ejakulasi paling singkat serta produksi spermatozoa paling rendah. Hubungan signifikan antara waktu reaksi dengan TS serta TSM pada individu Bizzyard dengan koefisien determinasi (R2) sebesar 14 % dan 20%. Hubungan sangat signifikan antara lama ejakulasi dengan TS serta TSM pada individu Cipta dengan R2 sebesar 46% dan 58%. Kesimpulan penelitian adalah terdapat hubungan antara tingkah laku seksual dengan produksi spermatozoa pada sapi Brahman, dengan koefisien korelasi (r) tertinggi sebesar 0,72 dan R2 sebesar 58% antara lama ejakulasi dan total spermatozoa motil pada individu Cipta. (The relationship between sexual behavior and semen production in Brahman bulls) ABSTRACT. Bulls for artificial insemination must be selected based on libido and sperm production. The study aimed to analyze the relationship between sexual behavior with sperm production on Brahman bulls. The material was 3 Brahman bulls collected using artificial vagina. The method was observational by observing reaction time and ejaculation duration. Laboratory observations by observing volume, concentration, and motility to calculate total sperm (TS) and total motile sperm counts (TMSC). Observations were replicated 10 times. The data were analyzed descriptively, the relationship between behavior and sperm production was examined using Pearson correlation. The results showed that there were variations in sexual behavior and sperm production in each bull. Cipta have the shortest reaction time and ejaculation duration and the lowest sperm production. A significant relationship between reaction time and TS and TMSC in Bizzyard with coefficient of determination (R2) of 14% and 20%. A very significant between ejaculation duration with TS and TMSC in Cipta with R2 of 46% and 58%. In conclusion, there was a relationship between sexual behavior and sperm production in Brahman bulls, with the highest correlation coefficient (r) of 0.72 and R2 of 58% between ejaculation duration and the total motile sperm counts in Cipta.

ABSTRAK. Penelitian ini bertujuan untuk menghitung nilai korelasi antara bobot badan dengan ukuran tubuh serta mengestimasi bobot badan (BB) berdasarkan ukuran tubuh. Materi penelitian ini adalah 159 ekor sapi Aceh umur sapih dan umur satu tahun. Metode penelitian adalah studi kasus berdasarkan data bobot sapih dan bobot satu tahun beserta ukuran tubuh sapi Aceh. Hasil penelitian pada sapi Aceh umur sapih menunjukkan korelasi bobot badan dengan lingkar dada, panjang badan, dan tinggi pundak berturut-turut 0.65, 0,56 dan 0,64, sedangkan nilai determinasinya adalah 0,42, 0,30 dan 0,41. Model regresi untuk estimasi bobot badan umur sapih yaitu BB= -30,23 + 0,47LD + 0,13PB + 0,50TP. Untuk sapi Aceh umur satu tahun nilai korelasi bobot badan dengan lingkar dada, panjang badan, dan tinggi pundak berturut-turut 0.69, 0,58 dan 0,55, nilai determinasinya adalah 0,47, 0,34 dan 0,30. Persamaan garis regresi untuk sapi Aceh umur satu tahun yaitu BB = -44,96 + 0,72LD + 0,46PB + 0,24TP. Berdasarkan hasil analisis korelasi menunjukkan bahwa korelasi lingkar dada dengan bobot badan memiliki hubungan paling kuat dibandingkan dengan panjang badan dan tinggi pundak. (Correlation between body size and body weight of Aceh Cattle at weaning and yearling age) ABSTRACT. This study aimed to evaluate the correlation between body weight and body size, as well to estimate body weight based on body size. A total of 159 Aceh cattle at weaning and yearling age were used in this study. The method used was a case study based on the data on weaning weight, yearling weight, and body size of Aceh cattle. The results showed that at the weaning age, the correlation values between body weight (BW) with chest circumference (CC), body length (BL), and shoulder height (SH) were 0.65, 0.56, and 0.64, respectively, while the determinant values were 0.42, 0.30, and 0.41, respectively. Regression model for estimating body weight at weaning age was BW = -30.23 + 0.47CC + 0.13BL + 0.50SH. At the yearling age, the correlation values of body weight with chest circumference, body length, and shoulder height were 0.69, 0.58, and 0.55, respectively, while the determinant values were 0.47, 0.34, and 0.30, respectively. The regression equation for yearling Aceh cattle was BW = -44.96 + 0.72CC + 0.46BL + 0.24SH. This study showed that the highest correlation was observed on the body weight with chest circumference compared to those with either body length or shoulder height.

mplant contraception is one type of contraceptive in the form of an implant made of silastik rubber containing the progestin hormone (levonogestrel) attached to the upper arm. This contraceptives are effective enough to prevent pregnancy in long-term with a lifetime of 3 years for 2 sticks and 1 stick implant. The main principle of levonorgestrel implants was to inhibite the ovulation resulting suppression of estrogen production. Estrogen is one of the important factors in bone remodeling. This becomes our concerns about the effect of implant use on the bone health status of the acceptors. The objective of this study is to determine mean difference of bone mineral density of the Lumbar Spine L1-L4 and Femur Neck on childbearing age woman who are implant acceptors and non hormonal family planning acceptors. This study was analytic survey with cross sectional design. The population in this study were all childbearing age woman in the work area of South Klaten Public Health Center. The sample were 30 people using purposive sampling. Data analysis was performed using Unpaired T-test with p ≤ 0,05. The result of examination on implant acceptor group totaling 15 people shown that the average of T-Score Lumbar Spine L1-L4 bone density was 0,51 and T-score of Femur neck bone was 0,1. While the results of bone density examination in the group of acceptor non hormonal totaling 15 people shown that the average value of T-score Lumbar Spine L1-L4 was 0.72, whereas the mean value of T-score of femur neck bone was 0.06. Unpaired T-Test statistic test for difference of bumble density of Lumbar Spine L1-L4 and Femur Neck between the two groups obtained P value 0.611 and 0.889 (P> 0,05). So there is no statistically significant difference between Lumbar spine L1-L4 and Femur neck bone density on childbearing age woman who are non-hormonal and implant acceptors. Keyword: Bone density, Lumbar Spine L1-L4, Femur neck, Implant, non hormonal Contraceptive

Abstract: The purpose of this research was to investigate the successful difference of artificial insemination (AI) using frozen semen and liquid semen with CEP-3 + egg yolk diluent of Ongole Crossbred cow indicated by Non Return Rate (NRR) and Conception Rate (CR). The materials used were 54 heads of cow which selected purposively using some criteria such as healthy, free from any reproduction disorder and having at least sexual desire criteria 3A (red, swollen and warm). This field experiment consisted of direct observation and interview to acquire primary and secondary data. The datas were analyzed descriptively and continued by Chi-square test. The results showed that the value of NRR was insignificant influenced between the two treatment. The Conception Rate for frozen semen and liquid semen was found 62.96% and 51.85% respectively. The conclusion of this experiment was the successful rate of artificial insemination (AI) using liquid semen with the CEP-3 + 20% egg yolk diluent was lower than artificial insemination using frozen semen. The value of Conception Rate of frozen semen treatment was 62.96% and the liquid semen treatment was 51.85%.

A total of 106 late gestation goats (age 4-5 months of pregnancy) records each of Etawah Crosbred goats kept on small farmer over a period of 10 years were assessed to determine the effects of environmental factors on them. Body Condition Score is non-genetic factor and in dairy goats to predicted milk production, fertility, and general health of the animal because BCS is visualisasi from feeding manajement. The objective of this study was to investigated the relationship between Body Condition Score (BCS) of late gestation with litter size and birth weight on etawah crossbred goats. The results showed that the average of litter size and birth weight (kg) were 1.89±0.66 and 3.84±0.73 respectively. The relationship between BCS with litter size were 0.13 (very low) and the relationship between BCS with birth weight were 0.11 (very low). The conclusion of this research is BCS of late gestation had positive correlation on litter size and birth weight, however this was low and birth weight as first indicator of future growth rate.

Although Etawah Crossbred (PE) goat is considered to be dual purpose (meat and milk) goat, it is mainly raised for meat production. Since early 1990, there has been a growing interest of the farmer in some places to raise PE goat for milk production without sacrificing its role to produce kids for meat. Although milk yield of PE goat was not as high as milk yield of some other dairy goats, the ability of PE goat to cope with harsh local environment, particularly climate and feed conditions, was an advantage. Therefore, raising PE goat would still be an important part of farmer activities in the rural areas in Indonesia. Identification of the genes underlying livestock production traits leads to more efficient breeding programs and it is a promising way to improve production traits of farm animals. Growth hormone is a polypeptide hormone which is the major regulator of the metabolic procedures of growth and development and it is encoded by GH gene. The objective of this study was to detect the genetic polymorphism of GH gene in major Etawah Crossbred (PE) goat using PCR-RFLP. The PCR amplified fragment were digested with HaeIII endonuclease and the result showed the presence of two genotype CC and CD. The total frequency were 47.0% and 53.0% for CC and CD genotype respectively in 94 tested goats. Statistical analysis showed that in the fragment amplified by the pair of primer, CD genotype had significant higher birth weight and weight of 100 days old (weaning weight) than CC genotype (P