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Your search keyword '"Kuwamoto, Satoshi"' showing total 249 results
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249 results on '"Kuwamoto, Satoshi"'

2. Imaging of Liver Tissues Using a Combination of Silver Impregnation and Low-Vacuum Scanning Electron Microscopy; A Simple Method for the High-Resolution Visualization of Reticulin Structures with Applicability to a Quantitative Analysis

Author

Izuta, Mikihiro, Kuwamoto, Satoshi, Kamiya, Tetsutaro, Nagata, Keiko, Matsushita, Michiko, Inaga, Sumire, Kaido, Toshiyuki, Umekita, Yoshihisa, Hayashi, Kazuhiko, and Kitamura, Yukisato

Subjects
electron, hepatocellular, scanning, reticulin, liver diseases, computer-assisted, microscopy, Original Article, carcinoma, image processing
Abstract

BACKGROUND: Low-vacuum scanning electron microscopy (LVSEM) enables the detailed three-dimensional imaging of archival tissues without special pretreatments. The clinical utility of LVSEM in the assessment of liver diseases has not yet been defined. So, we investigated the utility of LVSEM imaging in morphological assessments of normal and diseased liver tissues, with a focus on reticulin structures. METHODS: Formalin-fixed tissue samples of two normal livers and two hepatocellular carcinomas with background regenerative nodules/areas were stained with platinum blue stain or silver-impregnated using Watanabe’s method and then comparatively observed under LVSEM. We also evaluated the applicability of LVSEM imaging of liver tissues to a quantitative analysis using a digital image analysis technique. RESULTS: Optimal high-resolution images of reticulin structures were obtained using 10-μm-thick silver-impregnated sections. Reticulin fibers were clearly observed to run dendritically around sinusoids in normal livers, and markedly increased in regenerative nodules/areas. Normal reticulin frameworks were lost in hepatocellular carcinoma, leaving a few fragments of reticulin fibers within tumors. Moreover, when a quantitative analysis was applied to these images, we successfully demonstrated a significantly higher reticulin fiber density in regenerative nodules/areas than in the normal liver (P < 0.05). CONCLUSION: We not only obtained detailed three-dimensional images of reticulin structures in various liver tissues by LVSEM combined with silver impregnation but also showed their applicability to a quantitative analysis. The method presented herein may be applied to future studies for the more accurate diagnosis and better classification/risk stratification of various liver diseases.

Published
2023

4. Chronic Active Epstein?Barr Virus Infection Indistinguishable from Autoimmune Hepatitis: A Case Report

Author

Ikeda, Suguru, Sugihara, Takaaki, Kihara, Takuya, Hoshino, Yoshiki, Matsuki, Yukako, Nagahara, Takakazu, Oyama, Kenji, Okano, Jun-ichi, Kuwamoto, Satoshi, Horie, Yasushi, and Isomoto, Hajime

Subjects
Epstein–Barr virus, autoimmune hepatitis, EBV-encoded small RNA1, General Medicine, Patient Report, chronic active EBV infection
Abstract

Chronic active Epstein–Barr virus (CAEBV) infection is a rare disease, mainly affecting children, typically characterized by persistent infectious mononucleosis (IM)-like symptoms. We describe an adult case of CAEBV without IM-like symptoms, which was indistinguishable from autoimmune hepatitis (AIH). A 60-year-old woman with liver damage was diagnosed with AIH (International Diagnostic Score: 16 points). She had been treated with prednisolone for three years; however, her transaminases had never normalized. She was admitted for another liver biopsy due to repeated high fevers and worsening of her liver damage over two months. Her EBV-DNA copy number was 2.9 × 10(4) copies/μg DNA, and EBV-encoded small RNA1-positive lymphocytic infiltration was observed in both the present and previously collected (three years ago) liver tissue samples. This case implies that hepatic involvement in a CAEBV without IM-like symptoms is difficult to distinguish from AIH and may be misdiagnosed. In some steroid resistant AIH cases, evaluating for CAEBV may be valuable.

Published
2022

6. Development of a Prognostic Scoring System using MYC Expression and Soluble Interleukin Receptor -2 level for Diffuse Large B-cell Lymphoma

Author

Suzuki, Sayaka, primary, Kuwamoto, Satoshi, additional, Kawamura, Koji, additional, Matsushita, Michiko, additional, Motokura, Toru, additional, Hosoda, Yuzuru, additional, Maegaki, Masaya, additional, Hosoda, Rina, additional, Hara, Kentaro, additional, Umekita, Yoshihisa, additional, and Fukuda, Tetsuya, additional

Published
2023
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7. Epstein-Barr virus reactivation in peripheral B lymphocytes induces IgM-type thyrotropin receptor autoantibody production in patients with Graves’ disease

Author

Nagata, Keiko, primary, Hayashi, Kazuhiko, additional, Kumata, Keisuke, additional, Satoh, Yukio, additional, Osaki, Mitsuhiko, additional, Nakayama, Yuji, additional, Kuwamoto, Satoshi, additional, Ichihara, Yoshinori, additional, Okura, Tsuyoshi, additional, Matsuzawa, Kazuhiko, additional, Miake, Junichiro, additional, Fukata, Shuji, additional, and Imamura, Takeshi, additional

Published
2023
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10. Merkel cell polyomavirus and Langerhans cell neoplasm

Author

Murakami, Ichiro, Wada, Noriko, Nakashima, Junko, Iguchi, Mitsuko, Toi, Makoto, Hashida, Yumiko, Higuchi, Tomonori, Daibata, Masanori, Matsushita, Michiko, Iwasaki, Takeshi, Kuwamoto, Satoshi, Horie, Yasushi, Nagata, Keiko, Hayashi, Kazuhiko, Oka, Takashi, Yoshino, Tadashi, Imamura, Toshihiko, Morimoto, Akira, Imashuku, Shinsaku, Gogusev, Jean, and Jaubert, Francis

Published
2018
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16. An unresectable osteoblastoma of the axis controlled with denosumab

Author

Yamaga, Kensaku, primary, Kuwamoto, Satoshi, additional, Tanishima, Shinji, additional, Yamashita, Hideki, additional, Asano, Naofumi, additional, Matsushita, Michiko, additional, Akahori, Keiichi, additional, Osaki, Mari, additional, Hisaoka, Masanori, additional, and Nagashima, Hideki, additional

Published
2022
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32. Merkel cell polyomavirus–negative Merkel cell carcinoma is associated with JAK‐STAT and MEK‐ERK pathway activation.

Author

Iwasaki, Takeshi, Hayashi, Kazuhiko, Matsushita, Michiko, Nonaka, Daisuke, Kohashi, Kenichi, Kuwamoto, Satoshi, Umekita, Yoshihisa, and Oda, Yoshinao

Abstract

Merkel cell polyomavirus (MCPyV) is monoclonally integrated into the genomes of approximately 80% of Merkel cell carcinomas (MCCs). While the presence of MCPyV affects the clinicopathological features of MCC, the molecular mechanisms of MCC pathogenesis after MCPyV infection are unclear. This study investigates the association between MCPyV infection and activation of the MEK‐ERK and JAK‐STAT signaling pathways in MCC to identify new molecular targets for MCC treatment. The clinicopathological characteristics of 30 MCPyV‐positive and 20 MCPyV‐negative MCC cases were analyzed. The phosphorylation status of MEK, ERK, JAK, and STAT was determined by immunohistochemical analysis. The activation status of the MEK‐ERK and JAK‐STAT pathways and the effects of a JAK inhibitor (ruxolitinib) was analyzed in MCC cell lines. Immunohistochemically, the expression of pJAK2 (P =.038) and pERK1/2 (P =.019) was significantly higher in MCPyV‐negative than in MCPyV‐positive MCCs. Male gender (hazard ratio [HR] 2.882, P =.039), older age (HR 1.137, P <.001), negative MCPyV status (HR 0.324, P =.013), and advanced cancer stage (HR 2.672, P =.041) were identified as unfavorable prognostic factors; however, the phosphorylation states of JAK2, STAT3, MEK1/2, and ERK1/2 were unrelated to the prognosis. The inhibition of cell proliferation by ruxolitinib was greater in MCPyV‐negative MCC cell lines than in an MCPyV‐positive MCC cell line. The expression of pERK1/2 and pMEK was higher in MCPyV‐negative than in MCPyV‐positive cell lines. These results suggest that activation of the JAK2 and MEK‐ERK pathways was more prevalent in MCPyV‐negative than in MCPyV‐positive MCC and the JAK inhibitor ruxolitinib inhibited MEK‐ERK pathway activation. Consequently, the JAK‐STAT and MEK‐ERK signaling pathways may be potential targets for MCPyV‐negative MCC treatment. [ABSTRACT FROM AUTHOR]

Published
2022
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37. Identification of human herpes virus 1 encoded microRNAs in biopsy samples of lower esophageal sphincter muscle during peroral endoscopic myotomy for esophageal achalasia

Author

Ikebuchi, Yuichiro, primary, Kanda, Tsutomu, additional, Ikeda, Haruo, additional, Yoshida, Akira, additional, Sakaguchi, Takuki, additional, Urabe, Shigetoshi, additional, Minami, Hitomi, additional, Nakao, Kazuhiko, additional, Kuwamoto, Satoshi, additional, Inoue, Haruhiro, additional, and Isomoto, Hajime, additional

Published
2019
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42. Expression of the IDO1/TDO2-AhR pathway in tumor cells or the tumor microenvironment is associated with Merkel cell polyomavirus status and prognosis in Merkel cell carcinoma

Author

Wardhani, Lusi Oka, primary, Matsushita, Michiko, additional, Iwasaki, Takeshi, additional, Kuwamoto, Satoshi, additional, Nonaka, Daisuke, additional, Nagata, Keiko, additional, Kato, Masako, additional, Kitamura, Yukisato, additional, and Hayashi, Kazuhiko, additional

Published
2019
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46. Possible Relationship Between MYBL1 Alterations and Specific Primary Sites in Adenoid Cystic Carcinoma: A Clinicopathological and Molecular Study of 36 Cases

Author

Endo, Yukari, primary, Kuwamoto, Satoshi, additional, Ohira, Takahito, additional, Matsushita, Michiko, additional, Matsushige, Takahiro, additional, Fukuhara, Takahiro, additional, Nakamoto, Shu, additional, Hayashi, Kazuhiko, additional, Kugoh, Hiroyuki, additional, and Hirooka, Yasuaki, additional

Published
2019
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49. Epstein–Barr Virus Lytic Reactivation Induces IgG4 Production by Host B Lymphocytes in Graves' Disease Patients and Controls: A Subset of Graves' Disease Is an IgG4-Related Disease-Like Condition

Author

Nagata, Keiko, primary, Hara, Sayuri, additional, Nakayama, Yuji, additional, Higaki, Katsumi, additional, Sugihara, Hirotsugu, additional, Kuwamoto, Satoshi, additional, Matsushita, Michiko, additional, Kato, Masako, additional, Tanio, Shunsuke, additional, Ishiguro, Kiyosuke, additional, and Hayashi, Kazuhiko, additional

Published
2018
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