1. [Optimization of method for detecting the activation of potassium channels in rat cardiac muscle cells].
- Author
-
Wang X, Zhang L, Zhou S, and Zou W
- Subjects
- Animals, Blotting, Western methods, Cell Membrane metabolism, Female, Immunoprecipitation methods, Kv1.2 Potassium Channel analysis, Kv1.2 Potassium Channel genetics, Kv1.5 Potassium Channel analysis, Kv1.5 Potassium Channel genetics, Male, Phosphorylation, Rats, Rats, Sprague-Dawley, Kv1.2 Potassium Channel metabolism, Kv1.5 Potassium Channel metabolism, Myocytes, Cardiac cytology, Myocytes, Cardiac metabolism
- Abstract
To detect the phosphorylation of potassium channel protein (Kv1.2 and Kv1.5) in rat cardiac muscle cells accurately, we applied the combined method of immunoprecipitation and Western blot in this study. Compared with using Western blot alone, the combination of immunoprecipitation and Western blot displayed high sensitivity to detect the activation of potassium channel proteins. Because of its simplicity, quickness and reproducibility, we find that this method was promising for detecting the phosphorylation of Kv1.2 and Kv1.5 proteins or other potassium channel proteins in rat cardiac muscle cells.
- Published
- 2008