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5. Abstracts of presentations on plant protection issues at the xth international congress of virology: August 11–16, 1996 Binyanei haOoma, Jerusalem Iarael part 3(final part)

Author

Liu, Sijun, Bedford, Ian D., Markham, Peter G., Ghanim, Morad, Zeidan, Muhamad, Czosnek, Henryk, Bruyère, A., Herrbach, E., Brault, V., Ziegler-Graff, V., Guilley, H., van den Heuvel, J. F. J. M., Taiwo, M. A., Dijkstra, J., Martinez, B., López-Moya, J. J., Llave, C., Díaz-Ruíz, J. R., López-Abella, D., Mikoshiba, Y., Honda, K., Kanematsu, S., Fujisawa, I., Salomon, Raffi, Bernardi, Francoise, Raccah, B., Singer, S., Gal-On, A., Huet, H., López-Moya, J. J., Pirone, T. P., Visser, Peter B., Bol, John F., Hernández, Carmen, Brown, Derek J. F., Pappu, H. R., Culbreath, A. K., Todd, J. W., McPherson, R. M., Sherwood, J. L., Bertrand, P. F., Robbins, M. A., Reade, R. D., Rochon, D. M., Schönfelder, M., Körbler, M., Barg, E., Lesemann, D. -E., Vetten, H. J., Manqussopoulos, I. N., Tsagris, M., Maiss, E., Marczewski, W., Syller, J., Romero, Javier, Molina-Garcia, Antonio, Babin, Mar, Bujarski, Jozef J., Pogany, Judy, Zhang, L., Palukaitis, P., Kaplan, I. B., Qu, Feng, Morris, T. Jack, Steinkellner, H., Puehringer, H., Machado, A. M. Laimer da Câmara, Hammond, J., Brandt, S., Katinger, H., Himmler, G., Carrier, K., Hans, F., Wang, A., Sanfacon, H., Palkovics, László, Balázs, Ervin, Petrzik, K., Mráz, I., Fránová-Honetšlegrová, J., Kusiak, C., Berthome, R., Dinant, S., Astier, S., Albouy, J., Renou, J. P., Bó, E. Dal, Torre, M. E. Sánchez de la, Djelouah, K., García, M. L., Grau, O., Benvenisti, Luna, Gelman, Boris, Hai, Dalia, Yadin, Hagai, Stram, Yehuda, Becker, Yechiel, Čeřovská, N., Filigarová, M., Dědič, P., Nemchinov, L., Hadidi, A., Choi, Y. G., Randles, J. W., Samson, A. C. R., Wilford, J. N., Chapman, S., Santa Cruz, S., Wilson, T. M. A., Wilkinson, Nicola, Wilson, Louise, Marlow, Susan, King, Linda, Possee, Robert, Zhu, Fanxiu, Qi, Yipeng, Huang, Yongxiu, Hu, Jianhong, Oker-Blom, Christian, Keinänen, Kari, Chauhan, B. K., Possee, R. D., French, T. J., Finkelstein, Y., Levi, B. Z., Faktor, O., Toister-Achituv, Mira, Wang, Fushan, Qi, Yipeng, Huang, Yongxiu, Lu, Liquan, Du, Quansheng, Watson, S. K., Kalmakoff, J., Broer, R., Liu, Y., Zuidema, D., Strien, E. A. van, Vlak, J. M., Heldens, J. G. M., Chejanovsky, N., Gershburg, E., Toister-Achituv, Mira, Faruchi, S., Kamensky, B., Faktor, O., Faktor, O., Nahum, O., Stockholm, D., Rivkin, H., Gurevitz, M., Chejanovsky, N., Zilberberg, N., Gershburg, E., Stockholm, D., Rivkin, H., Chejanovsky, N., Gurevitz, M., Zilberberg, N., Gershburg, E., Smith, P., King, L. A., Bamett, A., Windass, J. D., Possee, R. D., Jacobs, C., Fielding, B., Davison, S., Kunjeku, E., Guarino, L. A., Jarvis, D. L., Reilly, L., Hoover, K., Schultz, C. M., Hammock, B. D., Gordon, K. H. J., Bawden, A. L., Brooks, E. M., Lincoln, M. R., Hanzlik, T. N., Larkin, P. J., Gordon, K. H. J., Bawden, A. L., van Hulten, M. C. W., Hanzlik, T. N., Hendry, D. A., Stephens, Rachel, Barnett, Anna, Thomas, Carole, Possee, Robert, King, Linda, Phanis, Constantinos, O’Reilly, David R., Clarke, E., Tristem, Michael, Cory, Jennifer, O’Reilly, David R., Mayo, M. A., Duncan, G. H., Reavy, B., Gildow, F. E., Lamb, J. W., Hay, R. T., Li, Shoudong, Qi, Bing, Wang, Jiawang, Qi, Yipeng, O’Reilly, David R., Kang, WonKyung, Crook, Norman E., Winstanley, Doreen, Alaoui-Ismaili, M. H., Richardson, C. D., Lundsgaard, T., Kobayashi, J., Kayama, T., Ikeda, N., Miyajima, S., Inouye, K., Kimura, T., Suzuki, N., Sugawara, M., Nuss, D. L., Matsuura, Y., Simón, Laureano, Guo, Huishan, García, Juan Antonio, Wang, S., Miller, W. A., Browning, K., Fütterer, Johannes, Potrykus, Ingo, Bao, Yiming, Li, Liu, Burns, Thomas M., Hull, Roger, Hohn, Thomas, Hefferon, K. L., AbouHaider, M. G., Hulanicka, D., Juszczuk, M., Iskakov, B. K., Shmanov, M. A., Polimbetova, N. S., Zhanybekova, S. Sh., Lee, A. V., Galiakparov, N. N., Dale, J. L., Beetham, P. R., Hafner, G. J., Harding, R. M., and Dale, J. L.

Published
1998
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7. Correction

Author

Liu, Sijun, Bedford, Ian, Markham, Peter, Ghanim, Morad, Zeidan, Muhamad, Czosnek, Henryk, Bruyère, A., Herrbach, E., Brault, V., Ziegler-Graff, V., Guilley, H., van den Heuvel, J., Taiwo, M., Dijkstra, J., Martinez, B., López-Moya, J., Llave, C., Díaz-Ruíz, J., López-Abella, D., Mikoshiba, Y., Honda, K., Kanematsu, S., Fujisawa, I., Salomon, Raffi, Bernardi, Francoise, Raccah, B., Singer, S., Gal-On, A., Huet, H., Pirone, T., Visser, Peter, Bol, John, Hernández, Carmen, Brown, Derek, Pappu, H., Culbreath, A., Todd, J., McPherson, R., Sherwood, J., Bertrand, P., Robbins, M., Reade, R., Rochon, D., Schönfelder, M., Körbler, M., Barg, E., Lesemann, D., Vetten, H., Manqussopoulos, I., Tsagris, M., Maiss, E., Marczewski, W., Syller, J., Romero, Javier, Molina-Garcia, Antonio, Babin, Mar, Bujarski, Jozef, Pogany, Judy, Zhang, L., Palukaitis, P., Kaplan, I., Qu, Feng, Morris, T., Steinkellner, H., Puehringer, H., Machado, A., Hammond, J., Brandt, S., Katinger, H., Himmler, G., Carrier, K., Hans, F., Wang, A., Sanfacon, H., Palkovics, László, Balázs, Ervin, Petrzik, K., Mráz, I., Fránová-Honetšlegrová, J., Kusiak, C., Berthome, R., Dinant, S., Astier, S., Albouy, J., Renou, J., Bó, E., Torre, M., Djelouah, K., García, M., Grau, O., Benvenisti, Luna, Gelman, Boris, Hai, Dalia, Yadin, Hagai, Stram, Yehuda, Becker, Yechiel, Čeřovská, N., Filigarová, M., Dědič, P., Nemchinov, L., Hadidi, A., Choi, Y., Randles, J., Samson, A., Wilford, J., Chapman, S., Santa Cruz, S., Wilson, T., Wilkinson, Nicola, Wilson, Louise, Marlow, Susan, King, Linda, Possee, Robert, Zhu, Fanxiu, Qi, Yipeng, Huang, Yongxiu, Hu, Jianhong, Oker-Blom, Christian, Keinänen, Kari, Chauhan, B., Possee, R., French, T., Finkelstein, Y., Levi, B., Faktor, O., Toister-Achituv, Mira, Wang, Fushan, Lu, Liquan, Du, Quansheng, Watson, S., Kalmakoff, J., Broer, R., Liu, Y., Zuidema, D., Strien, E., Vlak, J., Heldens, J., Chejanovsky, N., Gershburg, E., Faruchi, S., Kamensky, B., Nahum, O., Stockholm, D., Rivkin, H., Gurevitz, M., Zilberberg, N., Smith, P., King, L., Bamett, A., Windass, J., Jacobs, C., Fielding, B., Davison, S., Kunjeku, E., Guarino, L., Jarvis, D., Reilly, L., Hoover, K., Schultz, C., Hammock, B., Gordon, K., Bawden, A., Brooks, E., Lincoln, M., Hanzlik, T., Larkin, P., van Hulten, M., Hendry, D., Stephens, Rachel, Barnett, Anna, Thomas, Carole, Phanis, Constantinos, O'Reilly, David, Clarke, E., Tristem, Michael, Cory, Jennifer, Mayo, M., Duncan, G., Reavy, B., Gildow, F., Lamb, J., Hay, R., Li, Shoudong, Qi, Bing, Wang, Jiawang, Kang, WonKyung, Crook, Norman, Winstanley, Doreen, Alaoui-Ismaili, M., Richardson, C., Lundsgaard, T., Kobayashi, J., Kayama, T., Ikeda, N., Miyajima, S., Inouye, K., Kimura, T., Suzuki, N., Sugawara, M., Nuss, D., Matsuura, Y., Simón, Laureano, Guo, Huishan, García, Juan, Wang, S., Miller, W., Browning, K., Fütterer, Johannes, Potrykus, Ingo, Bao, Yiming, Li, Liu, Burns, Thomas, Hull, Roger, Hohn, Thomas, Hefferon, K., AbouHaider, M., Hulanicka, D., Juszczuk, M., Iskakov, B., Shmanov, M., Polimbetova, N., Zhanybekova, S., Lee, A., Galiakparov, N., Dale, J., Beetham, P., Hafner, G., and Harding, R.

Published
2018

8. Abstracts of presentations on plant protection issues at the xth international congress of virology: August 11-16, 1996 Binyanei haOoma, Jerusalem Iarael part 3(final part)

Author

Liu, Sijun, Bedford, Ian, Markham, Peter, Ghanim, Morad, Zeidan, Muhamad, Czosnek, Henryk, Bruyère, A., Herrbach, E., Brault, V., Ziegler-Graff, V., Guilley, H., van den Heuvel, J., Taiwo, M., Dijkstra, J., Martinez, B., López-Moya, J., Llave, C., Díaz-Ruíz, J., López-Abella, D., Mikoshiba, Y., Honda, K., Kanematsu, S., Fujisawa, I., Salomon, Raffi, Bernardi, Francoise, Raccah, B., Singer, S., Gal-On, A., Huet, H., Pirone, T., Visser, Peter, Bol, John, Hernández, Carmen, Brown, Derek, Pappu, H., Culbreath, A., Todd, J., McPherson, R., Sherwood, J., Bertrand, P., Robbins, M., Reade, R., Rochon, D., Schönfelder, M., Körbler, M., Barg, E., Lesemann, D., Vetten, H., Manqussopoulos, I., Tsagris, M., Maiss, E., Marczewski, W., Syller, J., Romero, Javier, Molina-Garcia, Antonio, Babin, Mar, Bujarski, Jozef, Pogany, Judy, Zhang, L., Palukaitis, P., Kaplan, I., Qu, Feng, Morris, T., Steinkellner, H., Puehringer, H., Machado, A., Hammond, J., Brandt, S., Katinger, H., Himmler, G., Carrier, K., Hans, F., Wang, A., Sanfacon, H., Palkovics, László, Balázs, Ervin, Petrzik, K., Mráz, I., Fránová-Honetšlegrová, J., Kusiak, C., Berthome, R., Dinant, S., Astier, S., Albouy, J., Renou, J., Bó, E., Torre, M., Djelouah, K., García, M., Grau, O., Benvenisti, Luna, Gelman, Boris, Hai, Dalia, Yadin, Hagai, Stram, Yehuda, Becker, Yechiel, Čeřovská, N., Filigarová, M., Dědič, P., Nemchinov, L., Hadidi, A., Choi, Y., Randles, J., Samson, A., Wilford, J., Chapman, S., Santa Cruz, S., Wilson, T., Wilkinson, Nicola, Wilson, Louise, Marlow, Susan, King, Linda, Possee, Robert, Zhu, Fanxiu, Qi, Yipeng, Huang, Yongxiu, Hu, Jianhong, Oker-Blom, Christian, Keinänen, Kari, Chauhan, B., Possee, R., French, T., Finkelstein, Y., Levi, B., Faktor, O., Toister-Achituv, Mira, Wang, Fushan, Lu, Liquan, Du, Quansheng, Watson, S., Kalmakoff, J., Broer, R., Liu, Y., Zuidema, D., Strien, E., Vlak, J., Heldens, J., Chejanovsky, N., Gershburg, E., Faruchi, S., Kamensky, B., Nahum, O., Stockholm, D., Rivkin, H., Gurevitz, M., Zilberberg, N., Smith, P., King, L., Bamett, A., Windass, J., Jacobs, C., Fielding, B., Davison, S., Kunjeku, E., Guarino, L., Jarvis, D., Reilly, L., Hoover, K., Schultz, C., Hammock, B., Gordon, K., Bawden, A., Brooks, E., Lincoln, M., Hanzlik, T., Larkin, P., van Hulten, M., Hendry, D., Stephens, Rachel, Barnett, Anna, Thomas, Carole, Phanis, Constantinos, O'Reilly, David, Clarke, E., Tristem, Michael, Cory, Jennifer, Mayo, M., Duncan, G., Reavy, B., Gildow, F., Lamb, J., Hay, R., Li, Shoudong, Qi, Bing, Wang, Jiawang, Kang, WonKyung, Crook, Norman, Winstanley, Doreen, Alaoui-Ismaili, M., Richardson, C., Lundsgaard, T., Kobayashi, J., Kayama, T., Ikeda, N., Miyajima, S., Inouye, K., Kimura, T., Suzuki, N., Sugawara, M., Nuss, D., Matsuura, Y., Simón, Laureano, Guo, Huishan, García, Juan, Wang, S., Miller, W., Browning, K., Fütterer, Johannes, Potrykus, Ingo, Bao, Yiming, Li, Liu, Burns, Thomas, Hull, Roger, Hohn, Thomas, Hefferon, K., AbouHaider, M., Hulanicka, D., Juszczuk, M., Iskakov, B., Shmanov, M., Polimbetova, N., Zhanybekova, S., Lee, A., Galiakparov, N., Dale, J., Beetham, P., Hafner, G., Harding, R., Liu, Sijun, Bedford, Ian, Markham, Peter, Ghanim, Morad, Zeidan, Muhamad, Czosnek, Henryk, Bruyère, A., Herrbach, E., Brault, V., Ziegler-Graff, V., Guilley, H., van den Heuvel, J., Taiwo, M., Dijkstra, J., Martinez, B., López-Moya, J., Llave, C., Díaz-Ruíz, J., López-Abella, D., Mikoshiba, Y., Honda, K., Kanematsu, S., Fujisawa, I., Salomon, Raffi, Bernardi, Francoise, Raccah, B., Singer, S., Gal-On, A., Huet, H., Pirone, T., Visser, Peter, Bol, John, Hernández, Carmen, Brown, Derek, Pappu, H., Culbreath, A., Todd, J., McPherson, R., Sherwood, J., Bertrand, P., Robbins, M., Reade, R., Rochon, D., Schönfelder, M., Körbler, M., Barg, E., Lesemann, D., Vetten, H., Manqussopoulos, I., Tsagris, M., Maiss, E., Marczewski, W., Syller, J., Romero, Javier, Molina-Garcia, Antonio, Babin, Mar, Bujarski, Jozef, Pogany, Judy, Zhang, L., Palukaitis, P., Kaplan, I., Qu, Feng, Morris, T., Steinkellner, H., Puehringer, H., Machado, A., Hammond, J., Brandt, S., Katinger, H., Himmler, G., Carrier, K., Hans, F., Wang, A., Sanfacon, H., Palkovics, László, Balázs, Ervin, Petrzik, K., Mráz, I., Fránová-Honetšlegrová, J., Kusiak, C., Berthome, R., Dinant, S., Astier, S., Albouy, J., Renou, J., Bó, E., Torre, M., Djelouah, K., García, M., Grau, O., Benvenisti, Luna, Gelman, Boris, Hai, Dalia, Yadin, Hagai, Stram, Yehuda, Becker, Yechiel, Čeřovská, N., Filigarová, M., Dědič, P., Nemchinov, L., Hadidi, A., Choi, Y., Randles, J., Samson, A., Wilford, J., Chapman, S., Santa Cruz, S., Wilson, T., Wilkinson, Nicola, Wilson, Louise, Marlow, Susan, King, Linda, Possee, Robert, Zhu, Fanxiu, Qi, Yipeng, Huang, Yongxiu, Hu, Jianhong, Oker-Blom, Christian, Keinänen, Kari, Chauhan, B., Possee, R., French, T., Finkelstein, Y., Levi, B., Faktor, O., Toister-Achituv, Mira, Wang, Fushan, Lu, Liquan, Du, Quansheng, Watson, S., Kalmakoff, J., Broer, R., Liu, Y., Zuidema, D., Strien, E., Vlak, J., Heldens, J., Chejanovsky, N., Gershburg, E., Faruchi, S., Kamensky, B., Nahum, O., Stockholm, D., Rivkin, H., Gurevitz, M., Zilberberg, N., Smith, P., King, L., Bamett, A., Windass, J., Jacobs, C., Fielding, B., Davison, S., Kunjeku, E., Guarino, L., Jarvis, D., Reilly, L., Hoover, K., Schultz, C., Hammock, B., Gordon, K., Bawden, A., Brooks, E., Lincoln, M., Hanzlik, T., Larkin, P., van Hulten, M., Hendry, D., Stephens, Rachel, Barnett, Anna, Thomas, Carole, Phanis, Constantinos, O'Reilly, David, Clarke, E., Tristem, Michael, Cory, Jennifer, Mayo, M., Duncan, G., Reavy, B., Gildow, F., Lamb, J., Hay, R., Li, Shoudong, Qi, Bing, Wang, Jiawang, Kang, WonKyung, Crook, Norman, Winstanley, Doreen, Alaoui-Ismaili, M., Richardson, C., Lundsgaard, T., Kobayashi, J., Kayama, T., Ikeda, N., Miyajima, S., Inouye, K., Kimura, T., Suzuki, N., Sugawara, M., Nuss, D., Matsuura, Y., Simón, Laureano, Guo, Huishan, García, Juan, Wang, S., Miller, W., Browning, K., Fütterer, Johannes, Potrykus, Ingo, Bao, Yiming, Li, Liu, Burns, Thomas, Hull, Roger, Hohn, Thomas, Hefferon, K., AbouHaider, M., Hulanicka, D., Juszczuk, M., Iskakov, B., Shmanov, M., Polimbetova, N., Zhanybekova, S., Lee, A., Galiakparov, N., Dale, J., Beetham, P., Hafner, G., and Harding, R.

Published
2018

9. Correction

Author

Liu, Sijun, Bedford, Ian D., Markham, Peter G., Ghanim, Morad, Zeidan, Muhamad, Czosnek, Henryk, Bruyère, A., Herrbach, E., Brault, V., Ziegler-Graff, V., Guilley, H., van den Heuvel, J. F. J. M., Taiwo, M. A., Dijkstra, J., Martinez, B., López-Moya, J. J., Llave, C., Díaz-Ruíz, J. R., López-Abella, D., Mikoshiba, Y., Honda, K., Kanematsu, S., Fujisawa, I., Salomon, Raffi, Bernardi, Francoise, Raccah, B., Singer, S., Gal-On, A., Huet, H., López-Moya, J. J., Pirone, T. P., Visser, Peter B., Bol, John F., Hernández, Carmen, Brown, Derek J. F., Pappu, H. R., Culbreath, A. K., Todd, J. W., McPherson, R. M., Sherwood, J. L., Bertrand, P. F., Robbins, M. A., Reade, R. D., Rochon, D. M., Schönfelder, M., Körbler, M., Barg, E., Lesemann, D. -E., Vetten, H. J., Manqussopoulos, I. N., Tsagris, M., Maiss, E., Marczewski, W., Syller, J., Romero, Javier, Molina-Garcia, Antonio, Babin, Mar, Bujarski, Jozef J., Pogany, Judy, Zhang, L., Palukaitis, P., Kaplan, I. B., Qu, Feng, Morris, T. Jack, Steinkellner, H., Puehringer, H., Machado, A. M. Laimer da Câmara, Hammond, J., Brandt, S., Katinger, H., Himmler, G., Carrier, K., Hans, F., Wang, A., Sanfacon, H., Palkovics, László, Balázs, Ervin, Petrzik, K., Mráz, I., Fránová-Honetšlegrová, J., Kusiak, C., Berthome, R., Dinant, S., Astier, S., Albouy, J., Renou, J. P., Bó, E. Dal, Torre, M. E. Sánchez de la, Djelouah, K., García, M. L., Grau, O., Benvenisti, Luna, Gelman, Boris, Hai, Dalia, Yadin, Hagai, Stram, Yehuda, Becker, Yechiel, Čeřovská, N., Filigarová, M., Dědič, P., Nemchinov, L., Hadidi, A., Choi, Y. G., Randles, J. W., Samson, A. C. R., Wilford, J. N., Chapman, S., Santa Cruz, S., Wilson, T. M. A., Wilkinson, Nicola, Wilson, Louise, Marlow, Susan, King, Linda, Possee, Robert, Zhu, Fanxiu, Qi, Yipeng, Huang, Yongxiu, Hu, Jianhong, Oker-Blom, Christian, Keinänen, Kari, Chauhan, B. K., Possee, R. D., French, T. J., Finkelstein, Y., Levi, B. Z., Faktor, O., Toister-Achituv, Mira, Wang, Fushan, Qi, Yipeng, Huang, Yongxiu, Lu, Liquan, Du, Quansheng, Watson, S. K., Kalmakoff, J., Broer, R., Liu, Y., Zuidema, D., Strien, E. A. van, Vlak, J. M., Heldens, J. G. M., Chejanovsky, N., Gershburg, E., Toister-Achituv, Mira, Faruchi, S., Kamensky, B., Faktor, O., Faktor, O., Nahum, O., Stockholm, D., Rivkin, H., Gurevitz, M., Chejanovsky, N., Zilberberg, N., Gershburg, E., Stockholm, D., Rivkin, H., Chejanovsky, N., Gurevitz, M., Zilberberg, N., Gershburg, E., Smith, P., King, L. A., Bamett, A., Windass, J. D., Possee, R. D., Jacobs, C., Fielding, B., Davison, S., Kunjeku, E., Guarino, L. A., Jarvis, D. L., Reilly, L., Hoover, K., Schultz, C. M., Hammock, B. D., Gordon, K. H. J., Bawden, A. L., Brooks, E. M., Lincoln, M. R., Hanzlik, T. N., Larkin, P. J., Gordon, K. H. J., Bawden, A. L., van Hulten, M. C. W., Hanzlik, T. N., Hendry, D. A., Stephens, Rachel, Barnett, Anna, Thomas, Carole, Possee, Robert, King, Linda, Phanis, Constantinos, O’Reilly, David R., Clarke, E., Tristem, Michael, Cory, Jennifer, O’Reilly, David R., Mayo, M. A., Duncan, G. H., Reavy, B., Gildow, F. E., Lamb, J. W., Hay, R. T., Li, Shoudong, Qi, Bing, Wang, Jiawang, Qi, Yipeng, O’Reilly, David R., Kang, WonKyung, Crook, Norman E., Winstanley, Doreen, Alaoui-Ismaili, M. H., Richardson, C. D., Lundsgaard, T., Kobayashi, J., Kayama, T., Ikeda, N., Miyajima, S., Inouye, K., Kimura, T., Suzuki, N., Sugawara, M., Nuss, D. L., Matsuura, Y., Simón, Laureano, Guo, Huishan, García, Juan Antonio, Wang, S., Miller, W. A., Browning, K., Fütterer, Johannes, Potrykus, Ingo, Bao, Yiming, Li, Liu, Burns, Thomas M., Hull, Roger, Hohn, Thomas, Hefferon, K. L., AbouHaider, M. G., Hulanicka, D., Juszczuk, M., Iskakov, B. K., Shmanov, M. A., Polimbetova, N. S., Zhanybekova, S. Sh., Lee, A. V., Galiakparov, N. N., Dale, J. L., Beetham, P. R., Hafner, G. J., Harding, R. M., and Dale, J. L.

Published
1998
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10. Integrated Management Of Insect Borne Viruses By Means Of Transmission Interference As An Alternative To Pesticides.

Author

Ciancio, A., Mukerji, K. G., FernáNdez-Calvino, L., LóPez-Abella, D., and LóPez-Moya, J. J.

Abstract

Viruses are important plant pathogens responsible of yield and quality losses in many crops. Most plant viruses are spread in nature surpassing plant defence barriers with the help of vector organisms, mainly insects. The application of pesticides is an insufficient strategy to stop virus dissemination and, in turn, it can cause important environmental damages. As a consequence, an active area of research is currently devoted to explore alternatives to the abuse of pesticides including, for instance, attempts to unravel the molecular mechanisms operating during insect transmission of plant viruses. All these efforts are aimed to design strategies of interference with the transmission process, which will eventually become part of Integrated Disease Management programmes for the control of virus pathogens. The present chapter reviews the available and potential means to interfere with transmission, and the prospects of such strategies. [ABSTRACT FROM AUTHOR]

Published
2007
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15. Correction

Author

Liu, Sijun, Bedford, Ian, Markham, Peter, Ghanim, Morad, Zeidan, Muhamad, Czosnek, Henryk, Bruyère, A., Herrbach, E., Brault, V., Ziegler-Graff, V., Guilley, H., van den Heuvel, J., Taiwo, M., Dijkstra, J., Martinez, B., López-Moya, J., Llave, C., Díaz-Ruíz, J., López-Abella, D., Mikoshiba, Y., Honda, K., Kanematsu, S., Fujisawa, I., Salomon, Raffi, Bernardi, Francoise, Raccah, B., Singer, S., Gal-On, A., Huet, H., Pirone, T., Visser, Peter, Bol, John, Hernández, Carmen, Brown, Derek, Pappu, H., Culbreath, A., Todd, J., McPherson, R., Sherwood, J., Bertrand, P., Robbins, M., Reade, R., Rochon, D., Schönfelder, M., Körbler, M., Barg, E., Lesemann, D., Vetten, H., Manqussopoulos, I., Tsagris, M., Maiss, E., Marczewski, W., Syller, J., Romero, Javier, Molina-Garcia, Antonio, Babin, Mar, Bujarski, Jozef, Pogany, Judy, Zhang, L., Palukaitis, P., Kaplan, I., Qu, Feng, Morris, T., Steinkellner, H., Puehringer, H., Machado, A., Hammond, J., Brandt, S., Katinger, H., Himmler, G., Carrier, K., Hans, F., Wang, A., Sanfacon, H., Palkovics, László, Balázs, Ervin, Petrzik, K., Mráz, I., Fránová-Honetšlegrová, J., Kusiak, C., Berthome, R., Dinant, S., Astier, S., Albouy, J., Renou, J., Bó, E., Torre, M., Djelouah, K., García, M., Grau, O., Benvenisti, Luna, Gelman, Boris, Hai, Dalia, Yadin, Hagai, Stram, Yehuda, Becker, Yechiel, Čeřovská, N., Filigarová, M., Dědič, P., Nemchinov, L., Hadidi, A., Choi, Y., Randles, J., Samson, A., Wilford, J., Chapman, S., Santa Cruz, S., Wilson, T., Wilkinson, Nicola, Wilson, Louise, Marlow, Susan, King, Linda, Possee, Robert, Zhu, Fanxiu, Qi, Yipeng, Huang, Yongxiu, Hu, Jianhong, Oker-Blom, Christian, Keinänen, Kari, Chauhan, B., Possee, R., French, T., Finkelstein, Y., Levi, B., Faktor, O., Toister-Achituv, Mira, Wang, Fushan, Lu, Liquan, Du, Quansheng, Watson, S., Kalmakoff, J., Broer, R., Liu, Y., Zuidema, D., Strien, E., Vlak, J., Heldens, J., Chejanovsky, N., Gershburg, E., Faruchi, S., Kamensky, B., Nahum, O., Stockholm, D., Rivkin, H., Gurevitz, M., Zilberberg, N., Smith, P., King, L., Bamett, A., Windass, J., Jacobs, C., Fielding, B., Davison, S., Kunjeku, E., Guarino, L., Jarvis, D., Reilly, L., Hoover, K., Schultz, C., Hammock, B., Gordon, K., Bawden, A., Brooks, E., Lincoln, M., Hanzlik, T., Larkin, P., van Hulten, M., Hendry, D., Stephens, Rachel, Barnett, Anna, Thomas, Carole, Phanis, Constantinos, O'Reilly, David, Clarke, E., Tristem, Michael, Cory, Jennifer, Mayo, M., Duncan, G., Reavy, B., Gildow, F., Lamb, J., Hay, R., Li, Shoudong, Qi, Bing, Wang, Jiawang, Kang, WonKyung, Crook, Norman, Winstanley, Doreen, Alaoui-Ismaili, M., Richardson, C., Lundsgaard, T., Kobayashi, J., Kayama, T., Ikeda, N., Miyajima, S., Inouye, K., Kimura, T., Suzuki, N., Sugawara, M., Nuss, D., Matsuura, Y., Simón, Laureano, Guo, Huishan, García, Juan, Wang, S., Miller, W., Browning, K., Fütterer, Johannes, Potrykus, Ingo, Bao, Yiming, Li, Liu, Burns, Thomas, Hull, Roger, Hohn, Thomas, Hefferon, K., AbouHaider, M., Hulanicka, D., Juszczuk, M., Iskakov, B., Shmanov, M., Polimbetova, N., Zhanybekova, S., Lee, A., Galiakparov, N., Dale, J., Beetham, P., Hafner, G., Harding, R., Liu, Sijun, Bedford, Ian, Markham, Peter, Ghanim, Morad, Zeidan, Muhamad, Czosnek, Henryk, Bruyère, A., Herrbach, E., Brault, V., Ziegler-Graff, V., Guilley, H., van den Heuvel, J., Taiwo, M., Dijkstra, J., Martinez, B., López-Moya, J., Llave, C., Díaz-Ruíz, J., López-Abella, D., Mikoshiba, Y., Honda, K., Kanematsu, S., Fujisawa, I., Salomon, Raffi, Bernardi, Francoise, Raccah, B., Singer, S., Gal-On, A., Huet, H., Pirone, T., Visser, Peter, Bol, John, Hernández, Carmen, Brown, Derek, Pappu, H., Culbreath, A., Todd, J., McPherson, R., Sherwood, J., Bertrand, P., Robbins, M., Reade, R., Rochon, D., Schönfelder, M., Körbler, M., Barg, E., Lesemann, D., Vetten, H., Manqussopoulos, I., Tsagris, M., Maiss, E., Marczewski, W., Syller, J., Romero, Javier, Molina-Garcia, Antonio, Babin, Mar, Bujarski, Jozef, Pogany, Judy, Zhang, L., Palukaitis, P., Kaplan, I., Qu, Feng, Morris, T., Steinkellner, H., Puehringer, H., Machado, A., Hammond, J., Brandt, S., Katinger, H., Himmler, G., Carrier, K., Hans, F., Wang, A., Sanfacon, H., Palkovics, László, Balázs, Ervin, Petrzik, K., Mráz, I., Fránová-Honetšlegrová, J., Kusiak, C., Berthome, R., Dinant, S., Astier, S., Albouy, J., Renou, J., Bó, E., Torre, M., Djelouah, K., García, M., Grau, O., Benvenisti, Luna, Gelman, Boris, Hai, Dalia, Yadin, Hagai, Stram, Yehuda, Becker, Yechiel, Čeřovská, N., Filigarová, M., Dědič, P., Nemchinov, L., Hadidi, A., Choi, Y., Randles, J., Samson, A., Wilford, J., Chapman, S., Santa Cruz, S., Wilson, T., Wilkinson, Nicola, Wilson, Louise, Marlow, Susan, King, Linda, Possee, Robert, Zhu, Fanxiu, Qi, Yipeng, Huang, Yongxiu, Hu, Jianhong, Oker-Blom, Christian, Keinänen, Kari, Chauhan, B., Possee, R., French, T., Finkelstein, Y., Levi, B., Faktor, O., Toister-Achituv, Mira, Wang, Fushan, Lu, Liquan, Du, Quansheng, Watson, S., Kalmakoff, J., Broer, R., Liu, Y., Zuidema, D., Strien, E., Vlak, J., Heldens, J., Chejanovsky, N., Gershburg, E., Faruchi, S., Kamensky, B., Nahum, O., Stockholm, D., Rivkin, H., Gurevitz, M., Zilberberg, N., Smith, P., King, L., Bamett, A., Windass, J., Jacobs, C., Fielding, B., Davison, S., Kunjeku, E., Guarino, L., Jarvis, D., Reilly, L., Hoover, K., Schultz, C., Hammock, B., Gordon, K., Bawden, A., Brooks, E., Lincoln, M., Hanzlik, T., Larkin, P., van Hulten, M., Hendry, D., Stephens, Rachel, Barnett, Anna, Thomas, Carole, Phanis, Constantinos, O'Reilly, David, Clarke, E., Tristem, Michael, Cory, Jennifer, Mayo, M., Duncan, G., Reavy, B., Gildow, F., Lamb, J., Hay, R., Li, Shoudong, Qi, Bing, Wang, Jiawang, Kang, WonKyung, Crook, Norman, Winstanley, Doreen, Alaoui-Ismaili, M., Richardson, C., Lundsgaard, T., Kobayashi, J., Kayama, T., Ikeda, N., Miyajima, S., Inouye, K., Kimura, T., Suzuki, N., Sugawara, M., Nuss, D., Matsuura, Y., Simón, Laureano, Guo, Huishan, García, Juan, Wang, S., Miller, W., Browning, K., Fütterer, Johannes, Potrykus, Ingo, Bao, Yiming, Li, Liu, Burns, Thomas, Hull, Roger, Hohn, Thomas, Hefferon, K., AbouHaider, M., Hulanicka, D., Juszczuk, M., Iskakov, B., Shmanov, M., Polimbetova, N., Zhanybekova, S., Lee, A., Galiakparov, N., Dale, J., Beetham, P., Hafner, G., and Harding, R.

16. Abstracts of presentations on plant protection issues at the xth international congress of virology: August 11-16, 1996 Binyanei haOoma, Jerusalem Iarael part 3(final part)

Author

Liu, Sijun, Bedford, Ian, Markham, Peter, Ghanim, Morad, Zeidan, Muhamad, Czosnek, Henryk, Bruyère, A., Herrbach, E., Brault, V., Ziegler-Graff, V., Guilley, H., van den Heuvel, J., Taiwo, M., Dijkstra, J., Martinez, B., López-Moya, J., Llave, C., Díaz-Ruíz, J., López-Abella, D., Mikoshiba, Y., Honda, K., Kanematsu, S., Fujisawa, I., Salomon, Raffi, Bernardi, Francoise, Raccah, B., Singer, S., Gal-On, A., Huet, H., Pirone, T., Visser, Peter, Bol, John, Hernández, Carmen, Brown, Derek, Pappu, H., Culbreath, A., Todd, J., McPherson, R., Sherwood, J., Bertrand, P., Robbins, M., Reade, R., Rochon, D., Schönfelder, M., Körbler, M., Barg, E., Lesemann, D., Vetten, H., Manqussopoulos, I., Tsagris, M., Maiss, E., Marczewski, W., Syller, J., Romero, Javier, Molina-Garcia, Antonio, Babin, Mar, Bujarski, Jozef, Pogany, Judy, Zhang, L., Palukaitis, P., Kaplan, I., Qu, Feng, Morris, T., Steinkellner, H., Puehringer, H., Machado, A., Hammond, J., Brandt, S., Katinger, H., Himmler, G., Carrier, K., Hans, F., Wang, A., Sanfacon, H., Palkovics, László, Balázs, Ervin, Petrzik, K., Mráz, I., Fránová-Honetšlegrová, J., Kusiak, C., Berthome, R., Dinant, S., Astier, S., Albouy, J., Renou, J., Bó, E., Torre, M., Djelouah, K., García, M., Grau, O., Benvenisti, Luna, Gelman, Boris, Hai, Dalia, Yadin, Hagai, Stram, Yehuda, Becker, Yechiel, Čeřovská, N., Filigarová, M., Dědič, P., Nemchinov, L., Hadidi, A., Choi, Y., Randles, J., Samson, A., Wilford, J., Chapman, S., Santa Cruz, S., Wilson, T., Wilkinson, Nicola, Wilson, Louise, Marlow, Susan, King, Linda, Possee, Robert, Zhu, Fanxiu, Qi, Yipeng, Huang, Yongxiu, Hu, Jianhong, Oker-Blom, Christian, Keinänen, Kari, Chauhan, B., Possee, R., French, T., Finkelstein, Y., Levi, B., Faktor, O., Toister-Achituv, Mira, Wang, Fushan, Lu, Liquan, Du, Quansheng, Watson, S., Kalmakoff, J., Broer, R., Liu, Y., Zuidema, D., Strien, E., Vlak, J., Heldens, J., Chejanovsky, N., Gershburg, E., Faruchi, S., Kamensky, B., Nahum, O., Stockholm, D., Rivkin, H., Gurevitz, M., Zilberberg, N., Smith, P., King, L., Bamett, A., Windass, J., Jacobs, C., Fielding, B., Davison, S., Kunjeku, E., Guarino, L., Jarvis, D., Reilly, L., Hoover, K., Schultz, C., Hammock, B., Gordon, K., Bawden, A., Brooks, E., Lincoln, M., Hanzlik, T., Larkin, P., van Hulten, M., Hendry, D., Stephens, Rachel, Barnett, Anna, Thomas, Carole, Phanis, Constantinos, O'Reilly, David, Clarke, E., Tristem, Michael, Cory, Jennifer, Mayo, M., Duncan, G., Reavy, B., Gildow, F., Lamb, J., Hay, R., Li, Shoudong, Qi, Bing, Wang, Jiawang, Kang, WonKyung, Crook, Norman, Winstanley, Doreen, Alaoui-Ismaili, M., Richardson, C., Lundsgaard, T., Kobayashi, J., Kayama, T., Ikeda, N., Miyajima, S., Inouye, K., Kimura, T., Suzuki, N., Sugawara, M., Nuss, D., Matsuura, Y., Simón, Laureano, Guo, Huishan, García, Juan, Wang, S., Miller, W., Browning, K., Fütterer, Johannes, Potrykus, Ingo, Bao, Yiming, Li, Liu, Burns, Thomas, Hull, Roger, Hohn, Thomas, Hefferon, K., AbouHaider, M., Hulanicka, D., Juszczuk, M., Iskakov, B., Shmanov, M., Polimbetova, N., Zhanybekova, S., Lee, A., Galiakparov, N., Dale, J., Beetham, P., Hafner, G., Harding, R., Liu, Sijun, Bedford, Ian, Markham, Peter, Ghanim, Morad, Zeidan, Muhamad, Czosnek, Henryk, Bruyère, A., Herrbach, E., Brault, V., Ziegler-Graff, V., Guilley, H., van den Heuvel, J., Taiwo, M., Dijkstra, J., Martinez, B., López-Moya, J., Llave, C., Díaz-Ruíz, J., López-Abella, D., Mikoshiba, Y., Honda, K., Kanematsu, S., Fujisawa, I., Salomon, Raffi, Bernardi, Francoise, Raccah, B., Singer, S., Gal-On, A., Huet, H., Pirone, T., Visser, Peter, Bol, John, Hernández, Carmen, Brown, Derek, Pappu, H., Culbreath, A., Todd, J., McPherson, R., Sherwood, J., Bertrand, P., Robbins, M., Reade, R., Rochon, D., Schönfelder, M., Körbler, M., Barg, E., Lesemann, D., Vetten, H., Manqussopoulos, I., Tsagris, M., Maiss, E., Marczewski, W., Syller, J., Romero, Javier, Molina-Garcia, Antonio, Babin, Mar, Bujarski, Jozef, Pogany, Judy, Zhang, L., Palukaitis, P., Kaplan, I., Qu, Feng, Morris, T., Steinkellner, H., Puehringer, H., Machado, A., Hammond, J., Brandt, S., Katinger, H., Himmler, G., Carrier, K., Hans, F., Wang, A., Sanfacon, H., Palkovics, László, Balázs, Ervin, Petrzik, K., Mráz, I., Fránová-Honetšlegrová, J., Kusiak, C., Berthome, R., Dinant, S., Astier, S., Albouy, J., Renou, J., Bó, E., Torre, M., Djelouah, K., García, M., Grau, O., Benvenisti, Luna, Gelman, Boris, Hai, Dalia, Yadin, Hagai, Stram, Yehuda, Becker, Yechiel, Čeřovská, N., Filigarová, M., Dědič, P., Nemchinov, L., Hadidi, A., Choi, Y., Randles, J., Samson, A., Wilford, J., Chapman, S., Santa Cruz, S., Wilson, T., Wilkinson, Nicola, Wilson, Louise, Marlow, Susan, King, Linda, Possee, Robert, Zhu, Fanxiu, Qi, Yipeng, Huang, Yongxiu, Hu, Jianhong, Oker-Blom, Christian, Keinänen, Kari, Chauhan, B., Possee, R., French, T., Finkelstein, Y., Levi, B., Faktor, O., Toister-Achituv, Mira, Wang, Fushan, Lu, Liquan, Du, Quansheng, Watson, S., Kalmakoff, J., Broer, R., Liu, Y., Zuidema, D., Strien, E., Vlak, J., Heldens, J., Chejanovsky, N., Gershburg, E., Faruchi, S., Kamensky, B., Nahum, O., Stockholm, D., Rivkin, H., Gurevitz, M., Zilberberg, N., Smith, P., King, L., Bamett, A., Windass, J., Jacobs, C., Fielding, B., Davison, S., Kunjeku, E., Guarino, L., Jarvis, D., Reilly, L., Hoover, K., Schultz, C., Hammock, B., Gordon, K., Bawden, A., Brooks, E., Lincoln, M., Hanzlik, T., Larkin, P., van Hulten, M., Hendry, D., Stephens, Rachel, Barnett, Anna, Thomas, Carole, Phanis, Constantinos, O'Reilly, David, Clarke, E., Tristem, Michael, Cory, Jennifer, Mayo, M., Duncan, G., Reavy, B., Gildow, F., Lamb, J., Hay, R., Li, Shoudong, Qi, Bing, Wang, Jiawang, Kang, WonKyung, Crook, Norman, Winstanley, Doreen, Alaoui-Ismaili, M., Richardson, C., Lundsgaard, T., Kobayashi, J., Kayama, T., Ikeda, N., Miyajima, S., Inouye, K., Kimura, T., Suzuki, N., Sugawara, M., Nuss, D., Matsuura, Y., Simón, Laureano, Guo, Huishan, García, Juan, Wang, S., Miller, W., Browning, K., Fütterer, Johannes, Potrykus, Ingo, Bao, Yiming, Li, Liu, Burns, Thomas, Hull, Roger, Hohn, Thomas, Hefferon, K., AbouHaider, M., Hulanicka, D., Juszczuk, M., Iskakov, B., Shmanov, M., Polimbetova, N., Zhanybekova, S., Lee, A., Galiakparov, N., Dale, J., Beetham, P., Hafner, G., and Harding, R.

21. The helper-component protease transmission factor of tobacco etch potyvirus binds specifically to an aphid ribosomal protein homologous to the laminin receptor precursor.

Author

Fernández-Calvino L, Goytia E, López-Abella D, Giner A, Urizarna M, Vilaplana L, and López-Moya JJ

Subjects
Animals, Aphids virology, Blotting, Far-Western, Mutant Proteins genetics, Mutant Proteins metabolism, Plant Diseases virology, Protein Binding, Protein Interaction Mapping, Receptors, Laminin genetics, Ribosomal Proteins genetics, Nicotiana virology, Viral Proteins genetics, Insect Proteins metabolism, Peptide Hydrolases metabolism, Potyvirus physiology, Ribosomal Proteins metabolism, Viral Proteins metabolism
Abstract

Potyviruses are plant pathogens transmitted by aphids in a non-persistent manner. During transmission, the virus-encoded factor helper-component protease (HCPro) is presumed to act as a molecular bridge, mediating the reversible retention of virions to uncharacterized binding sites in the vector mouthparts. Whilst the predicted interaction between HCPro and the coat protein (CP) of virions has been confirmed experimentally, the characterization of putative HCPro-specific receptors in aphids has remained elusive, with the exception of a report that described binding of HCPro of zucchini yellow mosaic virus to several cuticle proteins. To identify other aphid components that could play a role during transmission, this study used purified HCPro of tobacco etch virus (TEV) in far-Western blotting assays as bait to select interactors among proteins extracted from aphid heads. With this approach, new HCPro-interacting proteins were found, and several were identified after mass spectrometry analysis and searches in databases dedicated to aphid sequences. Among these interactors, a ribosomal protein S2 (RPS2) was chosen for further investigation due to its homology with the laminin receptor precursor, known to act as the receptor of several viruses. The specific interaction between RPS2 and TEV HCPro was confirmed after cloning and heterologous expression of the corresponding Myzus persicae gene. The possible involvement of RPS2 in the transmission process was further suggested by testing a variant of HCPro that was non-functional for transmission due to a mutation in the conserved KITC motif (EITC variant). This variant retained its ability to bind CP but failed to interact with RPS2.

Published
2010
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22. Production of plum pox virus HC-Pro functionally active for aphid transmission in a transient-expression system.

Author

Goytia E, Fernández-Calvino L, Martínez-García B, López-Abella D, and López-Moya JJ

Subjects
Animals, Cysteine Endopeptidases physiology, Plant Leaves metabolism, Plum Pox Virus chemistry, Potyvirus chemistry, Potyvirus pathogenicity, Recombinant Proteins biosynthesis, Rhizobium metabolism, Nicotiana metabolism, Viral Proteins physiology, Virulence, Aphids virology, Cysteine Endopeptidases biosynthesis, Insect Vectors virology, Plant Diseases virology, Plum Pox Virus pathogenicity, Protein Engineering methods, Viral Proteins biosynthesis
Abstract

Potyviruses are non-persistently transmitted by aphid vectors with the assistance of a viral accessory factor known as helper component (HC-Pro), a multifunctional protein that is also involved in many other essential processes during the virus infection cycle. A transient Agrobacterium-mediated expression system was used to produce Plum pox virus (PPV) HC-Pro in Nicotiana benthamiana leaves from constructs that incorporated the 5' region of the genome, yielding high levels of HC-Pro in agroinfiltrated leaves. The expressed PPV HC-Pro was able to assist aphid transmission of purified virus particles in a sequential feeding assay, and to complement transmission-defective variants of the virus. Also, HC-Pro of a second potyvirus, Tobacco etch virus (TEV), was expressed and found to be functional for aphid transmission. These results show that this transient system can be useful for production of functionally active HC-Pro in potyviruses, and the possible uses of this approach to study the mechanism of transmission are discussed.

Published
2006
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23. Structural analysis of tobacco etch potyvirus HC-pro oligomers involved in aphid transmission.

Author

Ruiz-Ferrer V, Boskovic J, Alfonso C, Rivas G, Llorca O, López-Abella D, and López-Moya JJ

Subjects
Cysteine Endopeptidases chemistry, Cysteine Endopeptidases metabolism, Dimerization, Image Processing, Computer-Assisted, Microscopy, Electron, Plants virology, Ultracentrifugation, Viral Proteins chemistry, Viral Proteins metabolism, Cysteine Endopeptidases isolation & purification, Cysteine Endopeptidases ultrastructure, Potyvirus metabolism, Viral Proteins isolation & purification, Viral Proteins ultrastructure
Abstract

Oligomeric forms of the HC-Pro protein of the tobacco etch potyvirus (TEV) have been analyzed by analytical ultracentrifugation and single-particle electron microscopy combined with three-dimensional (3D) reconstruction. Highly purified HC-Pro protein was obtained from plants infected with TEV by using a modified version of the virus that incorporates a histidine tag at the HC-Pro N terminus (hisHC-Pro). The purified protein retained a high biological activity in solution when tested for aphid transmission. Sedimentation equilibrium showed that the hisHC-Pro preparations were heterogeneous in size. Sedimentation velocity confirmed the previous observation and revealed that the active protein solution contained several sedimenting species compatible with dimers, tetramers, hexamers, and octamers of the protein. Electron microscopy fields of purified protein showed particles of different sizes and shapes. The reconstructed 3D structures suggested that the observed particles could correspond to dimeric, tetrameric, and hexameric forms of the protein. A model of the interactions required for oligomerization of the HC-Pro of potyviruses is proposed.

Published
2005
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24. Expression of functionally active helper component protein of Tobacco etch potyvirus in the yeast Pichia pastoris.

Author

Ruiz-Ferrer V, Goytia E, Martínez-García B, López-Abella D, and López-Moya JJ

Subjects
Amino Acid Sequence, Animals, Aphids physiology, Base Sequence, Cysteine Endopeptidases chemistry, Genetic Vectors, Molecular Sequence Data, Pichia genetics, Potyvirus genetics, Recombinant Proteins metabolism, Viral Proteins chemistry, Virion metabolism, Cysteine Endopeptidases genetics, Cysteine Endopeptidases metabolism, Pichia metabolism, Potyvirus metabolism, Nicotiana virology, Viral Proteins genetics, Viral Proteins metabolism
Abstract

Tobacco etch potyvirus (TEV) is transmitted by aphids in a non-persistent manner with the assistance of a virus-encoded protein known as helper component (HC-Pro). To produce a biologically active form of recombinant TEV HC-Pro protein, heterologous expression in the methylotrophic yeast Pichia pastoris was used. A cDNA encoding the TEV HC-Pro region, fused to a Saccharomyces cerevisiae alpha-mating factor secretory peptide coding region, was inserted into the P. pastoris genome using a modified version of the pPIC9 vector. The expressed TEV HC-Pro protein was obtained directly from culture medium of recombinant yeast colonies; it was able to interact with TEV particles in a protein overlay binding assay, and also to assist aphid transmission of purified TEV particles to plants using the aphid Myzus persicae as vector. Our results indicate that P. pastoris provides a rapid and low-cost heterologous expression system that can be used to obtain biologically active potyvirus HC-Pro protein for in vitro transmission assays.

Published
2004
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25. Serological characterization of Hungarian plum pox virus isolates.

Author

López-Moya JJ, López-Abella D, Díaz-Rúiz JR, Martinez-Garcia B, and Gáborjányi R

Subjects
Antibodies, Monoclonal, Antigens, Viral analysis, Capsid analysis, Capsid chemistry, Enzyme-Linked Immunosorbent Assay, Hungary, Immunoblotting, Moldova, Plum Pox Virus genetics, Plum Pox Virus isolation & purification, Polymerase Chain Reaction, Serotyping, Spain, Plum Pox Virus classification
Abstract

Three Hungarian (no. 2, 4 and 9), and a Moldavian (K) plum pox virus isolates were compared with a characterized Spanish isolate (5.15) by RT-PCR, ELISA, dot-blot and Western blot analysis. Monoclonal antibodies prepared against the external, intermediate and internal sequences of the coat protein of the Spanish isolate were able to differentiate the four isolates. Hungarian isolate No. 2 proved to be serologically identical to the Spanish isolate, while No. 4 showed appreciable differences and No. 9 could be recognized only by the monoclonal antibodies representing the intermedial and internal parts of the coat protein. K isolate showed a more distant relationship to other isolates. Our experiment provided the first demonstration of the presence of D type isolates in Hungary.

Published
1997
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26. Transmission by aphids of a naturally non-transmissible plum pox virus isolate with the aid of potato virus Y helper component.

Author

López-Moya JJ, Canto T, Díaz-Ruíz JR, and López-Abella D

Subjects
Amino Acid Sequence, Animals, Aphids, Capsid physiology, Insect Vectors, Molecular Sequence Data, Plum Pox Virus isolation & purification, Sequence Homology, Amino Acid, Cysteine Endopeptidases, Plant Diseases virology, Plum Pox Virus pathogenicity, Viral Proteins physiology
Abstract

Two Spanish plum pox virus (PPV) isolates, 5.15 and 3.3, were used in transmission experiments involving the aphid vector Myzus persicae, with woody and herbaceous host plants. These isolates differ in the size of their coat protein (CP) and sequence analysis revealed that isolate 3.3 has a 15 amino acid deletion near the N terminus of the CP, affecting the same positions as in a previously reported non-aphid-transmissible PPV isolate from Germany. Aphid transmission experiments showed that isolate 5.15 was transmitted from infected plants whereas isolate 3.3 was not. In contrast, both isolates were readily aphid-transmitted when acquired through artificial membranes from purified virus preparations supplemented with purified helper component (HC) obtained from potato virus Y-infected plants. This indicates that non-transmissibility of isolate 3.3 may be due to a defect in the HC rather than in the CP.

Published
1995
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