Search

Your search keyword '"López-Granados, Eduardo"' showing total 276 results
Start Over Author "López-Granados, Eduardo"
276 results on '"López-Granados, Eduardo"'

3. Epigenetic profiling linked to multisystem inflammatory syndrome in children (MIS-C): A multicenter, retrospective study

Author

Davalos, Veronica, García-Prieto, Carlos A., Ferrer, Gerardo, Aguilera-Albesa, Sergio, Valencia-Ramos, Juan, Rodríguez-Palmero, Agustí, Ruiz, Montserrat, Planas-Serra, Laura, Jordan, Iolanda, Alegría, Iosune, Flores-Pérez, Patricia, Cantarín, Verónica, Fumadó, Victoria, Viadero, Maria Teresa, Rodrigo, Carlos, Méndez-Hernández, Maria, López-Granados, Eduardo, Colobran, Roger, Rivière, Jacques G., Soler-Palacín, Pere, Pujol, Aurora, and Esteller, Manel

Published
2022
Full Text
View/download PDF

4. Molecular assessment of splicing variants in a cohort of patients with inborn errors of immunity: methodological approach and interpretation remarks.

Author

Miguel Berenguel, Laura, Gianelli, Carla, Matas Pérez, Elisabet, del Rosal, Teresa, Méndez Echevarría, Ana, Robles Marhuenda, Ángel, Feito Rodríguez, Marta, Caballero Molina, Maria Teresa, Magallares García, Lorena, Sánchez Garrido, Brenda, Hita Díaz, Samantha, Allende Martínez, Luis, Nozal Aranda, Pilar, Cámara Hijón, Carmen, López Granados, Eduardo, Rodríguez Pena, Rebeca, and Bravo García-Morato, María

Subjects
MESSENGER RNA, GENETIC variation, MEDICAL literature, GENETIC translation, GENETIC algorithms
Abstract

Background: Splicing is the molecular mechanism to produce mature messenger RNA (mRNA) before its translation into protein. It is estimated that 50% of disease-causing mutations disrupt splicing, mostly of them affecting canonical positions. However, variants occurring in coding regions or deep-intronic variants can also affect splicing. In these cases, interpretation of the results may be challenging and molecular validation is required. Methods: The study includes 23 patients with splicing variants out of a cohort of 187 patients diagnosed with inborn errors of immunity (IEI). Clinical features and immunophenotypes are shown. Reverse transcription-polymerase chain reaction (RT-PCR) is the molecular assay employed for pathogenicity validation. Results: We detected 23 patients of 20 pedigrees with splicing variants in IEI genes, which constitutes the 12.3% of our cohort. In total, 21 splicing variants were analyzed, 10 of which had previously been reported in the literature and 11 novel ones. Among the 23 patients, 16 showed variants at canonical splice sites. Molecular validation was required only in the cases of genes of uncertain significance (GUS), high homology pseudogenes or incompatible clinical phenotype. Seven patients showed variants outside canonical positions. All of them needed molecular validation, with the exception of two patients, whose variants had previously been well characterized in the medical literature. Conclusion: This study shows the proportion of splicing variants in a cohort of IEI patients, providing their clinical phenotypic characteristics and the methodology used to validate the splicing defects. Based on the results, an algorithm is proposed to clarify when a splicing variant should be validated by complementary methodology and when, by contrast, it can be directly considered disease causing. [ABSTRACT FROM AUTHOR]

Published
2025
Full Text
View/download PDF

5. Single-cell Atlas of common variable immunodeficiency shows germinal center-associated epigenetic dysregulation in B-cell responses

Author

Rodríguez-Ubreva, Javier, Arutyunyan, Anna, Bonder, Marc Jan, Del Pino-Molina, Lucía, Clark, Stephen J., de la Calle-Fabregat, Carlos, Garcia-Alonso, Luz, Handfield, Louis-François, Ciudad, Laura, Andrés-León, Eduardo, Krueger, Felix, Català-Moll, Francesc, Rodríguez-Cortez, Virginia C., Polanski, Krzysztof, Mamanova, Lira, van Dongen, Stijn, Kiselev, Vladimir Yu., Martínez-Saavedra, María T., Heyn, Holger, Martín, Javier, Warnatz, Klaus, López-Granados, Eduardo, Rodríguez-Gallego, Carlos, Stegle, Oliver, Kelsey, Gavin, Vento-Tormo, Roser, and Ballestar, Esteban

Published
2022
Full Text
View/download PDF

6. Immunogenicity and reactogenicity of BNT162b2 booster in ChAdOx1-S-primed participants (CombiVacS): a multicentre, open-label, randomised, controlled, phase 2 trial

Author

Martínez de Soto, Lucía, Rodríguez Mariblanca, Amelia, Díaz García, Lucía, Ramírez García, Elena, Seco Meseguer, Enrique, Stewart Balbás, Stefan Mark, Marín Candón, Alicia, García García, Irene, Urroz Elizalde, Mikel, Monserrat Villatoro, Jaime, de la Rosa, Paula, Sanz García, Marta, López Crespo, Cristina, Mauleón Martínez, Vega, de Madariaga Castell, Raquel, Vitón Vara, Laura, García Rodríguez, Julio, Buño, Antonio, López Granados, Eduardo, Cámara, Carmen, Rey Cuevas, Esther, Ayllon García, Pilar, Jiménez González, María, Hernández Rubio, Victoria, Moraga Alapont, Paloma, Sánchez, Amparo, Prieto, Rocío, Llorente Gómez, Silvia, Miragall Roig, Cristina, Aparicio Marlasca, Marina, de la Calle, Fernando, Arsuaga, Marta, Duque, Blanca, Meijide, Susana, García de Vicuña, Aitor, Santorcuato, Ana, Expósito, Iraide, de Benito, Sara, Andia, Joseba, Castillo, Cristina, Irurzun, Esther, Camino, Jesús, Temprano, Mikel, Goikoetxea, Josune, Bustinza, Alazne, Larrea, Maialen, Gallego, Mikel, García-Vázquez, Dolores, de la Hoz, Ana Belén, Pérez-Nanclares, Gustavo, Pérez-Guzmán, Estíbaliz, Idoyaga, Eneko, Lamela, Adriana, Oteo, Jesús, Castillo de la Osa, María, Hernández Gutiérrez, Lourdes, Andrés Galván, María Elena, Calonge, Esther, Bermejo, Mercedes, de la Torre-Tarazona, Erick Humberto, Cascajero, Almudena, Fedele, Giovanni, Perea, Concepción, Cervera, Isabel, Bodega-Mayor, Irene, Montes-Casado, María, Portolés, Pilar, Baranda, Jana, Granés, Laura, Lazaar, Sulayman, Herranz, Sara, Mellado, María Eugènia, Tortajada, Marta, Malet, Montserrat, Quesada, Sebastiana, Vilella, Anna, Llupià, Anna, Olivé, Victoria, Trilla, Antoni, Gómez, Begoña, González, Elisenda, Romero, Sheila, Gámez, Francisco Javier, Casals, Cristina, Burunat, Laura, Castelló, Juan José, Fernández, Patricia, Bedini, Josep Lluís, Vila, Jordi, Aguilar, Carla, Altadill, Carmen, Armadans, Lluis, Borras-Bermejo, Blanca, Calonge, Julia, Camacho, Lina, Feliu, Anna, Gili, Gisela, Llorente, Cesar, Martínez-Gómez, Xavier, Otero-Romero, Susana, Palacio, Esther, Parés, Oleguer, Pinós, Laia, Plaza, Aitana, Riera-Arnau, Judit, Rodrigo-Pendás, José Angel, Sans, Carla, Santos, José, Torres, Gloria, Torrens, Margarita, Uriona, Sonia, Ballarin Alins, Elena, Pérez Esquirol, Eulàlia, Vendrell Bosch, Lourdes, Laredo Velasco, Leonor, Uribe López, Diana, González Rojano, Esperanza, Sánchez-Craviotto, Manuel, Rivas Paterna, Ana Belén, Hernán-Gómez, Teresa Iglesias, Rodríguez Galán, Natalia, Gil Marín, José Antonio, Álvarez-Morales, Verónica, Navalpotro, Ana Belén, Jiménez-Santamaría, M Dolores, Cardós, M Carmen, Hermoso, Elena, García-Arenillas, Mar, Pérez Macías, Natalia, Domingo Fernández, Alexandra, López Picado, Amanda, Quiñones, Jorge Mario, Deidda, Nicoletta, García-Franco, Ana, Torvisco, José María, Borobia, Alberto M, Carcas, Antonio J, Pérez-Olmeda, Mayte, Castaño, Luis, Bertran, María Jesús, García-Pérez, Javier, Campins, Magdalena, Portolés, Antonio, González-Pérez, María, García Morales, María Teresa, Arana-Arri, Eunate, Aldea, Marta, Díez-Fuertes, Francisco, Fuentes, Inmaculada, Ascaso, Ana, Lora, David, Imaz-Ayo, Natale, Barón-Mira, Lourdes E, Agustí, Antonia, Pérez-Ingidua, Carla, Gómez de la Cámara, Agustín, Arribas, José Ramón, Ochando, Jordi, Alcamí, José, Belda-Iniesta, Cristóbal, and Frías, Jesús

Published
2021
Full Text
View/download PDF

7. Coronavirus disease 2019 in patients with inborn errors of immunity: An international study

Author

Meyts, Isabelle, Bucciol, Giorgia, Quinti, Isabella, Neven, Bénédicte, Fischer, Alain, Seoane, Elena, Lopez-Granados, Eduardo, Gianelli, Carla, Robles-Marhuenda, Angel, Jeandel, Pierre-Yves, Paillard, Catherine, Sankaran, Vijay G., Demirdag, Yesim Yilmaz, Lougaris, Vassilios, Aiuti, Alessandro, Plebani, Alessandro, Milito, Cinzia, Dalm, Virgil ASH., Guevara-Hoyer, Kissy, Sánchez-Ramón, Silvia, Bezrodnik, Liliana, Barzaghi, Federica, Gonzalez-Granado, Luis Ignacio, Hayman, Grant R., Uzel, Gulbu, Mendonça, Leonardo Oliveira, Agostini, Carlo, Spadaro, Giuseppe, Badolato, Raffaele, Soresina, Annarosa, Vermeulen, François, Bosteels, Cedric, Lambrecht, Bart N., Keller, Michael, Mustillo, Peter J., Abraham, Roshini S., Gupta, Sudhir, Ozen, Ahmet, Karakoc-Aydiner, Elif, Baris, Safa, Freeman, Alexandra F., Yamazaki-Nakashimada, Marco, Scheffler-Mendoza, Selma, Espinosa-Padilla, Sara, Gennery, Andrew R., Jolles, Stephen, Espinosa, Yazmin, Poli, M. Cecilia, Fieschi, Claire, Hauck, Fabian, Cunningham-Rundles, Charlotte, Mahlaoui, Nizar, Warnatz, Klaus, Sullivan, Kathleen E., and Tangye, Stuart G.

Published
2021
Full Text
View/download PDF

8. Technical challenges of intracellular flow cytometry-based assays as a functional complement to diagnosis of signaling defects of inborn errors of immunity: PI3K pathway as a case of study.

Author

del Pino Molina, Lucía, Reche Yebra, Keren, Soto Serrano, Yolanda, Clemente Bernal, Álvaro, Avendaño-Monje, Carmen L., Ocejo-Vinyals, J. Gonzalo, Rodríguez Pena, Rebeca, and López Granados, Eduardo

Subjects
B cell receptors, GENETIC variation, B cells, FLOW cytometry, NUCLEOTIDE sequencing
Abstract

Background: The use of next-generation sequencing in inborn errors of immunity (IEI) has considerably increased the identification of novel gene variants, many of which are identified in patients without the described clinical phenotype or with variants of uncertain pathogenic significance in previously described genes. Properly designed functional and cellular assays, many necessarily accomplished by research-based laboratories, reveal the pathogenic consequences of the gene variants and contribute to diagnosis. Activated PI3Kδ syndrome (APDS) is a rare disease that can be divided into APDS1, caused by gain of function (GOF) mutations in PIK3CD gene, and APDS2, with loss of function (LOF) variants in the PIK3R1 gene. Both entities present hyperactivation of the PI3K pathway, which can be analyzed through Akt and S6 phosphorylation status. Methods: Our objective was to perform an accurate, robust, and reproducible functional assay to analyze the phosphorylation status of proteins in the PI3K-Akt-S6 pathway by flow cytometry, to contribute to diagnosis, to monitor treatments, and to establish intra-assay standardization. Results: We illustrate the robustness and reproducibility of our experimental procedure in patients with APDS who had high Akt and/or S6 phosphorylation levels at baseline, and after anti-IgM stimulation in B cells. We show the relevance of an appropriate cohort of samples from healthy donors, processed within the same conditions as the suspected samples, in particular the time frame for sample processing once blood is collected. Discussion: We highlight the importance of B cell stimulation through B cell receptor signaling, which is highly recommended, especially for samples that would be processed more than 24 hours after blood extraction. Also, having a defined experimental procedure is important, including the cytometer setup, which allows cytometer reproducibility for a period of time, enabling the comparison of a sample at different times. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

9. Epstein-Barr virus-specific T-cell response in pediatric liver transplant recipients: a cross-sectional study by multiparametric flow cytometry.

Author

Cuesta-Martín de la Cámara, Ricardo, Torices-Pajares, Andrea, Miguel-Berenguel, Laura, Reche-Yebra, Keren, Frauca-Remacha, Esteban, Hierro-Llanillo, Loreto, Muñoz-Bartolo, Gema, Lledín-Barbacho, María Dolores, Gutiérrez-Arroyo, Almudena, Martínez-Feito, Ana, López-Granados, Eduardo, and Sánchez-Zapardiel, Elena

Subjects
HISTOCOMPATIBILITY antigens, EPSTEIN-Barr virus diseases, CELL surface antigens, VIRAL load, CELLULAR immunity
Abstract

Background: Epstein-Barr virus (EBV) specific T-cell response measurement can help adjust immunosuppression in transplant patients with persistent infections. We aim to define T-cell responses against EBV in a cohort of pediatric liver-transplant patients. Methods: Thirty-eight immunosuppressed pediatric liver-transplant patients (IP) and 25 EBV-seropositive healthy-adult controls (HC) were included in our cross-sectional study. Based on their EBV serological (S) and viral load (VL) status, patients were categorized into IP-SNEG, IP-SPOSVLNEG and IP-SPOSVLPOS groups. T-cell response was assessed at two timepoints by stimulating cells with EBV peptides (PepTivator®) and performing intracellular-cytokine and activation-induced marker staining. Background subtraction was used to determine EBV-specific T-lymphocyte frequency. Results: Polyfunctional CD8+ T cells indicated previous EBV contact (IP-SNEG 0.00% vs IP-SPOS 0.04% and HC 0.02%; p=0.001 and p=0.01, respectively). Polyfunctional CD8+CD107a+IFNɣ+IL2-TNFα- profile was increased in serology-positive (IP-SNEG 0.01% vs IP-SPOS 0.13% and HC 0.03%; p=0.01 and p=0.50, respectively) and viral-load positive (IP-SPOSVLPOS 0.43% vs IP-SPOSVLNEG 0.07% and HC 0.03%; p=0.03 and p=0.001, respectively) patients. Central-memory cells were increased among serology-positive adults (IP-SNEG 0.00% vs IP-SPOS 0.13% and HC 4.33%; p=0.58 and p=0.002, respectively). At the second timepoint, IP-SNEG patients remained negative (first visit 0.01% vs second visit 0.00%, p=0.44). On the other hand, IP-SPOSVLPOS patients had cleared viral loads and, subsequently, decreased polyfunctional CD8+CD107a+IFNɣ+IL2-TNFα- cells (first visit 0.43% vs second visit 0.10%, p=0.81). Conclusion: Polyfunctional CD8+ EBV-specific T-cell response allows detecting EBV previous contact in liver-transplant children. %CD8+CD107a+IFNɣ+IL2-TNFα- is increased in patients with positive viral loads. Central memory CD4+ T-cell population more effectively determines prior EBV-exposure in adults. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

10. Executive Summary of the Consensus Document on the Diagnosis and Management of Patients with Primary Immunodeficiencies

Author

Cordero, Elisa, Goycochea-Valdivia, Walter, Mendez-Echevarria, Ana, Allende, Luis M., Alsina, Laia, Bravo García-Morato, Maria, Gil-Herrera, Juana, Gudiol, Carlota, Len-Abad, Oscar, López-Medrano, Francisco, Moreno-Pérez, David, Muñoz, Patricia, Olbrich, Peter, Sánchez-Ramón, Silvia, Soler-Palacín, Pere, Aguilera Cros, Clara, Arostegui, Juan Ignacio, Badell Serra, Isabel, Carbone, Javier, Fortún, Jesús, Gonzalez-Granado, Luis I., López-Granados, Eduardo, Lucena, José Manuel, Parody, Rocío, Ramakers, Jan, Regueiro, José R., Rivière, Jacques G., Roca-Oporto, Cristina, Rodríguez Pena, Rebeca, Santos-Pérez, Juan Luis, Rodríguez-Gallego, Carlos, and Neth, Olaf

Published
2020
Full Text
View/download PDF

11. Determining value in the treatment of activated PI3Kδ syndrome in Spain: a multicriteria decision analysis from the perspective of key stakeholders

Author

Gil, Alicia, primary, Reyes Abad, María, additional, Alerany, Carmen, additional, Alsina, Laia, additional, López Granados, Eduardo, additional, Neth, Olaf, additional, Poveda, José Luis, additional, Rivière, Jacques G., additional, Rodríguez-Gallego, Carlos, additional, Tutein Nolthenius, Joanne B., additional, Figueiredo, Raquel, additional, and Subías Labazuy, Silvia, additional

Published
2024
Full Text
View/download PDF

12. Severe Impairment of T-cell Immunity and Pulmonary GvHD Are Major Risk Factors for Nontuberculous Mycobacterial Infection After Pediatric Allogeneic Hematopoietic Stem Cell Transplantation

Author

Ascunce, Marina, primary, Coccolo, Alejandra, additional, Mozo, Yasmina, additional, Baquero-Artigao, Fernando, additional, Rodriguez-Molino, Paula, additional, Toro-Rueda, Carlos, additional, García-Clemente, Paloma, additional, Sánchez-Zapardiel, Elena, additional, López-Granados, Eduardo, additional, Corral-Sánchez, Dolores, additional, Bueno, David, additional, Sisinni, Luisa, additional, Pérez-Martínez, Antonio, additional, Calvo, Cristina, additional, and del Rosal, Teresa, additional

Published
2024
Full Text
View/download PDF

15. This title is unavailable for guests, please login to see more information.

Author

Gil, Alicia, Reyes Abad, María, Alerany, Carmen, Alsina, Laia, López Granados, Eduardo, Neth, Olaf, Poveda, José Luis, Rivière, Jacques G., Rodríguez-Gallego, Carlos, Tutein Nolthenius, Joanne B., Figueiredo , Raquel, Subías Labazuy , Silvia, Gil, Alicia, Reyes Abad, María, Alerany, Carmen, Alsina, Laia, López Granados, Eduardo, Neth, Olaf, Poveda, José Luis, Rivière, Jacques G., Rodríguez-Gallego, Carlos, Tutein Nolthenius, Joanne B., Figueiredo , Raquel, and Subías Labazuy , Silvia

Published
2024

16. The lack of either IRF9, or STAT2, has surprisingly little effect on human natural killer cell development and function

Author

Instituto de Salud Carlos III, Secretaría de Estado de Investigación, Desarrollo e Innovación (España), 0000-0001-6917-8980, 0000-0002-7065-2923, 0000-0003-2855-1595, Calvo-Apalategi, Ane, Nevado, Marta López, Bravo-Gallego, Luz Yadira, González-Granado, Luis Ignacio, Allende, Luis M, Pena, Rebeca Rodríguez, López-Granados, Eduardo, Reyburn, Hugh T, Instituto de Salud Carlos III, Secretaría de Estado de Investigación, Desarrollo e Innovación (España), 0000-0001-6917-8980, 0000-0002-7065-2923, 0000-0003-2855-1595, Calvo-Apalategi, Ane, Nevado, Marta López, Bravo-Gallego, Luz Yadira, González-Granado, Luis Ignacio, Allende, Luis M, Pena, Rebeca Rodríguez, López-Granados, Eduardo, and Reyburn, Hugh T

Abstract

Analysis of genetically defined immunodeficient patients allows study of the effect of the absence of specific proteins on human immune function in real-world conditions. Here we have addressed the importance of type I interferon signalling for human NK cell development by studying the phenotype and function of circulating NK cells isolated from patients suffering primary immunodeficiency disease due to mutation of either the human interferon regulatory factor 9 (IRF9) or the signal transducer and activator of transcription 2 (STAT2) genes. IRF9, together with phosphorylated STAT1 and STAT2, form a heterotrimer called interferon stimulated gene factor 3 (ISGF3) which promotes the expression of hundreds of IFN-stimulated genes that mediate antiviral function triggered by exposure to type I interferons. IRF9- and STAT2-deficient patients are unable to respond efficiently to stimulation by type I interferons and so our experiments provide insights into the importance of type I interferon signalling and the consequences of its impairment on human NK cell biology. Surprisingly, the NK cells of these patients display essentially normal phenotype and function.

Published
2024

18. Selection and validation of antibody clones against IgG and IgA subclasses in switched memory B-cells and plasma cells

Author

Blanco, Elena, Perez-Andres, Martin, Sanoja-Flores, Luzalba, Wentink, Marjolein, Pelak, Ondrej, Martín-Ayuso, Marta, Grigore, Georgiana, Torres-Canizales, Juan, López-Granados, Eduardo, Kalina, Tomas, van der Burg, Mirjam, Arriba-Méndez, Sonia, Santa Cruz, Santiago, Puig, Noemí, van Dongen, Jacques J.M., and Orfao, Alberto

Published
2019
Full Text
View/download PDF

19. Defects in memory B-cell and plasma cell subsets expressing different immunoglobulin-subclasses in patients with CVID and immunoglobulin subclass deficiencies

Author

Blanco, Elena, Pérez-Andrés, Martín, Arriba-Méndez, Sonia, Serrano, Cristina, Criado, Ignacio, Del Pino-Molina, Lucía, Silva, Susana, Madruga, Ignacio, Bakardjieva, Marina, Martins, Catarina, Serra-Caetano, Ana, Romero, Alfonso, Contreras-Sanfeliciano, Teresa, Bonroy, Carolien, Sala, Francisco, Martín, Alejandro, Bastida, José María, Lorente, Félix, Prieto, Carlos, Dávila, Ignacio, Marcos, Miguel, Kalina, Tomas, Vlkova, Marcela, Chovancova, Zita, Cordeiro, Ana Isabel, Philippé, Jan, Haerynck, Filomeen, López-Granados, Eduardo, Sousa, Ana E., van der Burg, Mirjam, van Dongen, Jacques J.M., and Orfao, Alberto

Published
2019
Full Text
View/download PDF

20. The lack of either IRF9, or STAT2, has surprisingly little effect on human natural killer cell development and function.

Author

Calvo‐Apalategi, Ane, Nevado, Marta López, Bravo‐Gallego, Luz Yadira, González‐Granado, Luis Ignacio, Allende, Luis M., Pena, Rebeca Rodríguez, López‐Granados, Eduardo, and Reyburn, Hugh T.

Subjects
KILLER cells, TYPE I interferons, STAT proteins, CELL physiology, INTERFERON regulatory factors
Abstract

Analysis of genetically defined immunodeficient patients allows study of the effect of the absence of specific proteins on human immune function in real‐world conditions. Here we have addressed the importance of type I interferon signalling for human NK cell development by studying the phenotype and function of circulating NK cells isolated from patients suffering primary immunodeficiency disease due to mutation of either the human interferon regulatory factor 9 (IRF9) or the signal transducer and activator of transcription 2 (STAT2) genes. IRF9, together with phosphorylated STAT1 and STAT2, form a heterotrimer called interferon stimulated gene factor 3 (ISGF3) which promotes the expression of hundreds of IFN‐stimulated genes that mediate antiviral function triggered by exposure to type I interferons. IRF9‐ and STAT2‐deficient patients are unable to respond efficiently to stimulation by type I interferons and so our experiments provide insights into the importance of type I interferon signalling and the consequences of its impairment on human NK cell biology. Surprisingly, the NK cells of these patients display essentially normal phenotype and function. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

22. Unexpected relevant role of gene mosaicism in patients with primary immunodeficiency diseases

Author

Mensa-Vilaró, Anna, Bravo García-Morato, María, de la Calle-Martin, Oscar, Franco-Jarava, Clara, Martínez-Saavedra, María Teresa, González-Granado, Luis I., González-Roca, Eva, Fuster, Jose Luis, Alsina, Laia, Mutchinick, Osvaldo M., Balderrama-Rodríguez, Angélica, Ramos, Eduardo, Modesto, Consuelo, Mesa-del-Castillo, Pablo, Ortego-Centeno, Norberto, Clemente, Daniel, Souto, Alejandro, Palmou, Natalia, Remesal, Agustín, Leslie, Kieron S., Gómez de la Fuente, Enrique, Yadira Bravo Gallego, Luz, Campistol, Josep María, Dhouib, Naouel Guirat, Bejaoui, Mohamed, Dutra, Lívia Almeida, Terreri, Maria Teresa, Mosquera, Catalina, González, Tatiana, Cañellas, Jerónima, García-Ruiz de Morales, José María, Wouters, Carine H., Bosque, María Teresa, Cham, Weng Tarng, Jiménez-Treviño, Santiago, de Inocencio, Jaime, Bloomfield, Markéta, Pérez de Diego, Rebeca, Martínez-Pomar, Natalia, Rodríguez-Pena, Rebeca, González-Santesteban, Cecilia, Soler-Palacín, Pere, Casals, Ferran, Yagüe, Jordi, Allende, Luis M., Rodríguez-Gallego, José Carlos, Colobran, Roger, Martínez-Martínez, Laura, López-Granados, Eduardo, and Aróstegui, Juan I.

Published
2019
Full Text
View/download PDF

23. Unexpected High Incidence of Human Herpesvirus-6 Encephalitis after Naive T Cell–Depleted Graft of Haploidentical Stem Cell Transplantation in Pediatric Patients

Author

Sisinni, Luisa, Gasior, Mercedes, de Paz, Raquel, Querol, Sergio, Bueno, David, Fernández, Lucia, Marsal, Julia, Sastre, Ana, Gimeno, Ramon, Alonso, Laura, Badell, Isabel, López-Granados, Eduardo, Torres, Juan, Medina, Laura, Torrent, Montserrat, Diaz de Heredia, Cristina, Escudero, Adela, and Pérez-Martínez, Antonio

Published
2018
Full Text
View/download PDF

25. DECTIN-1: A modifier protein in CTLA-4 haploinsufficiency

Author

Turnbull, Cynthia, primary, Bones, Josiah, additional, Stanley, Maurice, additional, Medhavy, Arti, additional, Wang, Hao, additional, Lorenzo, Ayla May D., additional, Cappello, Jean, additional, Shanmuganandam, Somasundhari, additional, Pandey, Abhimanu, additional, Seneviratne, Sandali, additional, Brown, Grant J, additional, Meng, Xiangpeng, additional, Fulcher, David, additional, Burgio, Gaetan, additional, Man, Si Ming, additional, de Lucas Collantes, Carmen, additional, Gasior, Mercedes, additional, López Granados, Eduardo, additional, Martin, Pilar, additional, Jiang, Simon H., additional, Cook, Matthew C., additional, Ellyard, Julia I., additional, Athanasopoulos, Vicki, additional, Corry, Ben, additional, Canete, Pablo F., additional, and Vinuesa, Carola G., additional

Published
2023
Full Text
View/download PDF

26. Predicting anti-TNF treatment response in rheumatoid arthritis: An artificial intelligence-driven model using cytokine profile and routine clinical practice parameters

Author

Valdivieso Shephard, Juan Luis, primary, Alvarez Robles, Enrique Josue, additional, Cámara Hijón, Carmen, additional, Hernandez Breijo, Borja, additional, Novella-Navarro, Marta, additional, Bogas Schay, Patricia, additional, Cuesta de la Cámara, Ricardo, additional, Balsa Criado, Alejandro, additional, López Granados, Eduardo, additional, and Plasencia Rodríguez, Chamaida, additional

Published
2023
Full Text
View/download PDF

27. The challenge of standardizing CAR‐T cell monitoring: A comparison of two flow‐cytometry methods and correlation with qPCR technique.

Author

Valdivieso‐Shephard, Juan Luis, Matas‐Pérez, Elisabet, García‐Bujalance, Silvia, Mirones‐Aguilar, Isabel, González‐Martínez, Berta, Pérez‐Martínez, Antonio, López‐Granados, Eduardo, Martínez‐Feito, Ana, and Sánchez‐Zapardiel, Elena

Abstract

Chimeric antigen receptor (CAR) T‐cell therapy is a breakthrough in hematologic malignancies, such as acute B lymphoblastic leukemia (B‐ALL). Monitoring this treatment is recommended, although standardized protocols have not been developed yet. This work compares two flow cytometry monitoring strategies and correlates this technique with qPCR method. CAR‐T cells were detected by two different flow‐cytometry protocols (A and B) in nine blood samples from one healthy donor and five B‐ALL patients treated with Tisagenlecleucel (Kymriah®, USA). HIV‐1 viral load allowed CAR detection by qPCR, using samples from seven healthy donors and nine B‐ALL patients. CAR detection by protocol A and B did not yield statistically significant differences (1.9% vs. 11.8% CD3 + CAR+, p = 0.07). However, protocol B showed a better discrimination of the CD3 + CAR+ population. A strong correlation was observed between protocol B and qPCR (r = 0.7, p < 0.0001). CD3 + CAR+ cells were detected by flow cytometry only when HIV‐1 viral load was above 104 copies/mL. In conclusion, protocol B was the most specific flow‐cytometry procedure for the identification of CAR‐T cells and showed a high correlation with qPCR. Further efforts are needed to achieve a standardized monitoring approach. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

29. Determining value in the treatment of activated PI3KÎ' syndrome in Spain: a multicriteria decision analysis from the perspective of key stakeholders.

Author

Abad, María Reyes, Alerany, Carmen, Alsina, Laia, López Granados, Eduardo, Neth, Olaf, Luis Poveda, Jose, Rivière, Jacques G., Rodríguez-Gallego, Carlos, Nolthenius, Joanne B. Tutein, Figueiredo, Raquel, Labazuy, Silvia Subías, and Gil, Alicia

Subjects
HEMATOPOIETIC stem cell transplantation, PHOSPHATIDYLINOSITOL 3-kinases, DECISION making, EXPERT evidence, DELAYED diagnosis
Abstract

Introduction: Activated phosphoinositide 3-kinase (PI3K)Î' syndrome (APDS) is an ultra-rare inborn error of immunity (IEI) combining immunodeficiency and immune dysregulation. This study determined what represents value in APDS in Spain from a multidisciplinary perspective applying multicriteria decision analysis (MCDA) methodology. Methods: A multidisciplinary committee of nine experts scored the evidence matrix. A specific framework for orphan drug evaluation in Spain and the weights assigned by a panel of 98 evaluators and decision-makers was used. Re-evaluation of scores was performed. Results: APDS is considered a very severe disease with important unmet needs, including misdiagnosis and diagnostic delay. Current management is limited to treatment of symptoms with off-label use of therapies supported by limited evidence. Therapeutic benefit is partial, resulting in limited disease control. Haematopoietic stem cell transplantation (HSCT), the only potential curative alternative, is restricted to a reduced patient population and without evidence of long-term efficacy or safety. All options present a limited safety profile. Data on patients' quality of life are lacking. APDS is associated with high pharmacological, medical and indirect costs. Conclusions: APDS is considered a severe disease, with limited understanding by key stakeholders of how treatment success is assessed in clinical practice, the serious impact that has on patients and the associated high economic burden. This study brings to light how MCDA methodology could represent a useful tool to complement current clinical and decision-making methods used by APDS experts and evaluators. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

30. Detection of specific RBD+ IgG+ memory B cells by flow cytometry in healthcare workers and patients with inborn errors of immunity after BNT162b2 m RNA COVID-19 vaccination

Author

del Pino Molina, Lucía, primary, Bravo Gallego, Luz Yadira, additional, Nozal, Pilar, additional, Soto-Serrano, Yolanda, additional, Martínez-Feito, Ana, additional, Reche-Yebra, Keren, additional, González-Torbay, Andrea, additional, Cuesta-Martín de la Cámara, Ricardo, additional, Gianelli, Carla, additional, Cámara, Carmen, additional, González-García, J., additional, González-Muñoz, Miguel, additional, Rodríguez-Pena, Rebeca, additional, and López Granados, Eduardo, additional

Published
2023
Full Text
View/download PDF

33. Familial CD45RA– T cells to treat severe refractory infections in immunocompromised patients

Author

Al-Akioui Sanz, Karima, primary, Echecopar Parente, Carlos, additional, Ferreras, Cristina, additional, Menéndez Ribes, Marta, additional, Navarro, Alfonso, additional, Mestre, Carmen, additional, Clares, Laura, additional, Vicario, José Luis, additional, Balas, Antonio, additional, De Paz, Raquel, additional, López Granados, Eduardo, additional, Sánchez Zapardiel, Elena, additional, Jiménez, Carlos, additional, López-Oliva, María, additional, Ramos, Esther, additional, Hernández-Oliveros, Francisco, additional, and Pérez-Martínez, Antonio, additional

Published
2023
Full Text
View/download PDF

34. Tittle: Cytokine Profile to Predict Remission in Rheumatoid Arthritis Patients Treated with Anti-Tnf, an Artificial Intelligence-Based Approach

Author

Valdivieso, Juan, primary, Alvarez-Robles, Enrique, additional, Cámara-Hijón, Carmen, additional, Hernandez-Breijo, Borja, additional, Novella Navarro, Marta, additional, Bogas-Schay, Patricia, additional, Cuesta-de-la-Cámara, Ricardo, additional, Balsa-Criado, Alejandro, additional, López-Granados, Eduardo, additional, and Plasencia-Rodríguez, Chamaida, additional

Published
2023
Full Text
View/download PDF

39. Humoral and cellular immune responses to Pfizer-BioNTech BNT162b2 SARS-CoV-2 vaccine in adolescents with liver transplantation: Single center experience

Author

Sánchez-Zapardiel, Elena, primary, Alós, María, additional, Nozal, Pilar, additional, González-Muñoz, Miguel, additional, Frauca-Remacha, Esteban, additional, Gavilán, Lucía Blanca, additional, Quiles, María José, additional, Hierro, Loreto, additional, and López-Granados, Eduardo, additional

Published
2022
Full Text
View/download PDF

40. Single-cell Atlas of common variable immunodeficiency shows germinal center-associated epigenetic dysregulation in B-cell responses.

Author

Generalitat de Catalunya, Fundación Josep Carreras, Ministerio de Ciencia e Innovación (España), Instituto de Salud Carlos III, Jeffrey Modell Foundation, Chan Zuckerberg Initiative, Wellcome Sanger Institute, European Commission, Biotechnology and Biological Sciences Research Council (UK), Medical Research Council (UK), Rodríguez-Ubreva, Javier, Arutyunyan, Anna, Bonder, Marc J., Pino-Molina, Lucía del, Clark, Stephen J., Calle-Fabregat, Carlos de la, García-Alonso, Luz, Handfield, Louis-François, Ciudad, Laura, Andrés-León, Eduardo, Krueger, Felix, Català-Moll, Francesc, Rodríguez-Cortez, Virginia C., Polanski, Krzysztof, Mamanova, Lira, Dongen, Stijn van, Kiselev, Vladimir Yu, Martínez-Saavedra, María T., Heyn, Holger, Martin, Javier, Warnatz, Klaus, López-Granados, Eduardo, Rodriguez-Gallego, Carlos, Stegle, Oliver, Kelsey, Gavin, Vento-Tormo, Roser, Ballestar, Esteban, Generalitat de Catalunya, Fundación Josep Carreras, Ministerio de Ciencia e Innovación (España), Instituto de Salud Carlos III, Jeffrey Modell Foundation, Chan Zuckerberg Initiative, Wellcome Sanger Institute, European Commission, Biotechnology and Biological Sciences Research Council (UK), Medical Research Council (UK), Rodríguez-Ubreva, Javier, Arutyunyan, Anna, Bonder, Marc J., Pino-Molina, Lucía del, Clark, Stephen J., Calle-Fabregat, Carlos de la, García-Alonso, Luz, Handfield, Louis-François, Ciudad, Laura, Andrés-León, Eduardo, Krueger, Felix, Català-Moll, Francesc, Rodríguez-Cortez, Virginia C., Polanski, Krzysztof, Mamanova, Lira, Dongen, Stijn van, Kiselev, Vladimir Yu, Martínez-Saavedra, María T., Heyn, Holger, Martin, Javier, Warnatz, Klaus, López-Granados, Eduardo, Rodriguez-Gallego, Carlos, Stegle, Oliver, Kelsey, Gavin, Vento-Tormo, Roser, and Ballestar, Esteban

Abstract

Common variable immunodeficiency (CVID), the most prevalent symptomatic primary immunodeficiency, displays impaired terminal B-cell differentiation and defective antibody responses. Incomplete genetic penetrance and ample phenotypic expressivity in CVID suggest the participation of additional pathogenic mechanisms. Monozygotic (MZ) twins discordant for CVID are uniquely valuable for studying the contribution of epigenetics to the disease. Here, we generate a single-cell epigenomics and transcriptomics census of naïve-to-memory B cell differentiation in a CVID-discordant MZ twin pair. Our analysis identifies DNA methylation, chromatin accessibility and transcriptional defects in memory B-cells mirroring defective cell-cell communication upon activation. These findings are validated in a cohort of CVID patients and healthy donors. Our findings provide a comprehensive multi-omics map of alterations in naïve-to-memory B-cell transition in CVID and indicate links between the epigenome and immune cell cross-talk. Our resource, publicly available at the Human Cell Atlas, gives insight into future diagnosis and treatments of CVID patients.

Published
2022

42. Clinical similarities and differences of patients with X-linked lymphoproliferative syndrome type 1 (XLP-1/SAP deficiency) versus type 2 (XLP-2/XIAP deficiency)

Author

Schmid, Jana Pachlopnik, Canioni, Danielle, Moshous, Despina, Touzot, Fabien, Mahlaoui, Nizar, Hauck, Fabian, Kanegane, Hirokazu, Lopez-Granados, Eduardo, Mejstrikova, Ester, Pellier, Isabelle, Galicier, Lionel, Galambrun, Claire, Barlogis, Vincent, Bordigoni, Pierre, Fourmaintraux, Alain, Hamidou, Mohamed, Dabadie, Alain, Le Deist, Françoise, Haerynck, Filomeen, Ouachée-Chardin, Marie, Rohrlich, Pierre, Stephan, Jean-Louis, Lenoir, Christelle, Rigaud, Stéphanie, Lambert, Nathalie, Milili, Michèle, Schiff, Claudin, Chapel, Helen, Picard, Capucine, de Saint Basile, Geneviève, Blanche, Stéphane, Fischer, Alain, and Latour, Sylvain

Published
2011
Full Text
View/download PDF

43. Hematopoietic stem cell transplantation for CD3δ deficiency

Author

Marcus, Nufar, Takada, Hidetoshi, Law, Jason, Cowan, Morton J., Gil, Juana, Regueiro, Jose R., Plaza Lopez de Sabando, Diego, Lopez-Granados, Eduardo, Dalal, Jignesh, Friedrich, Wilhelm, Manfred, Hoenig, Hanson, Imelda Celine, Grunebaum, Eyal, Shearer, William T., and Roifman, Chaim M.

Published
2011
Full Text
View/download PDF

45. Detection of specific RBD+ IgG+ memory B cells by flow cytometry in healthcare workers and patients with inborn errors of immunity after BNT162b2 m RNA COVID-19 vaccination.

Author

del Pino Molina, Lucía, Bravo Gallego, Luz Yadira, Nozal, Pilar, Soto-Serrano, Yolanda, Martínez-Feito, Ana, Reche-Yebra, Keren, González-Torbay, Andrea, Cuesta-Martín de la Cámara, Ricardo, Gianelli, Carla, Cámara, Carmen, González-García, J., González-Muñoz, Miguel, Rodríguez-Pena, Rebeca, and López Granados, Eduardo

Subjects
IMMUNOLOGIC memory, MEDICAL personnel, COVID-19 vaccines, FLOW cytometry, IMMUNITY, PSYCHONEUROIMMUNOLOGY
Abstract

Introduction: Inborn errors of immunity (IEI) are a heterogeneous group of diseases caused by intrinsic defects of the immune system. Estimating the immune competence of immunocompromised patients for an infection risk assessment or after SARS-CoV-2 vaccination constituted a challenge. Methods: The aim of this study was to determine the humoral responses of patients with IEI through a comprehensive analysis of specific receptor-binding domain-positive (RBD+) IgG+ memory B cells (MBCs) by flow cytometry, together with routine S-specific IgG antibodies and QuantiFERON SARS-CoV-2 (T-cell response), before the vaccine and 3 weeks after a second dose. Results and discussion: We first analyzed the percentage of specific RBD+ IgG+ MBCs in healthy healthcare workers. Within the control group, there was an increase in the percentage of specific IgG+ RBD+ MBCs 21 days after the second dose, which was consistent with S-specific IgG antibodies. Thirty-one patients with IEI were included for the pre- and post-vaccination study; IgG+ RBD+ MBCs were not evaluated in 6 patients due to an absence of B cells in peripheral blood. We detected various patterns among the patients with IEI with circulating B cells (25, 81%): an adequate humoral response was observed in 12/25, consider by the detection of positive S-specific IgG antibodies and the presence of specific IgG+ RBD+ MBCs, presenting a positive T-cell response; in 4/25, very low S-specific IgG antibody counts correlated with undetectable events in the IgG+ RBD+ MBC compartment but with positive cellular response. Despite the presence of Sspecific IgG antibodies, we were unable to detect a relevant percentage of IgG+ RBD+ MBCs in 5/25; however, all presented positive T-cell response. Lastly, we observed a profound failure of B and T-cell response in 3 (10%) patients with IEI, with no assessment of S-specific IgG antibodies, IgG+ RBD+ MBCs, and negative cellular response. The identification of specific IgG+ RBD+MBCs by flow cytometry provides information on different humoral immune response outcomes in patients with IEI and aids the assessment of immune competence status after SARS-CoV-2mRNA vaccine (BNT162b2), together with S-specific IgG antibodies and T-cell responses. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

48. Differential effects of the second SARS-CoV-2 mRNA vaccine dose on T cell immunity in naive and COVID-19 recovered individuals

Author

Lozano-Ojalvo, Daniel, Camara, Carmen, Lopez-Granados, Eduardo, Nozal, Pilar, del Pino-Molina, Lucía, Bravo-Gallego, Luz Yadira, Paz-Artal, Estela, Pion, Marjorie, Correa-Rocha, Rafael, Ortiz, Alberto, Lopez-Hoyos, Marcos, Iribarren, Marta Erro, Portoles, Jose, Rojo-Portoles, Maria Pilar, Ojeda, Gloria, Cervera, Isabel, Gonzalez-Perez, Maria, Bodega-Mayor, Irene, Montes-Casado, Maria, Portoles, Pilar, Perez-Olmeda, Mayte, Oteo, Jesus, Sanchez-Tarjuelo, Rodrigo, Pothula, Venu, Schwarz, Megan, Brahmachary, Manisha, Tan, Anthony Tanoto, Le Bert, Nina, Berin, Cecilia, Bertoletti, Antonio, Guccione, Ernesto, and Ochando, Jordi

Published
2021
Full Text
View/download PDF

49. Colaboradores

Author

Abajo Iglesias, Francisco J., Abalovich, Marcos S., Adamo, Bárbara, García-Navarro, Àlvar Agustí, García-Navarro, Carlos Agustí, Albarrán Artahona, Víctor, Alcalá Hernández, Luis, Alcaraz Asensio, Antonio, Alcázar Arroyo, Roberto, Alegre de Miquel, Víctor, Alejaldre Monforte, Aida, Almirante Gracera, Benito, del Pino Alonso Ortega, M.ª, Alonso Pérez, Manuel, Alonso-Tarrés, Carles, Manrique de Lara, Laia Alsina, Álvarez Larrán, Alberto, Álvarez-Martínez, Miriam José, Álvarez-Sala Walther, José Luís, Álvarez-Twose, Iván, Ambrosioni Czyrco, Juan, Amor Fernández, Antonio J., Ancochea Bermúdez, Julio, Andrade Bellido, Raúl, Andrés Collado, Mariano, Antón López, Jordi, Antón Nieto, Esperanza, Aran Perramon, Josep M.ª, Arance Fernández, Ana M.ª, Arias Gómez, Manuel, Arias Rivas, Susana, Arismendi Núñez, Ebymar, Ariza Cardenal, Xavier, Armengol Dulcet, Lluís, Aróstegui Gorospe, Juan Ignacio, Arranz Amo, José Antonio, Arrese, Marco, Audí Parera, Laura, Aulinas Masó, Anna, Badia Jobal, Joan Ramon, Badimon Maestro, Lina, Baena Caparrós, Jacinto, Baenas Soto, Isabel M.ª, Baeza Pertegaz, Inmaculada, Balcells Oliveró, Mercè, Martínez de Irujo, Jaume Baldirà, Barbé Illa, Ferran Eduard, Barberà Mir, Joan Albert, Barberán López, José, Barile Fabrís, Leonor A., Bartra Tomás, Joan, Bassas Arnau, Lluis, Bassat, Quique, Basté Rotllan, Neus, Bataller Alberola, Luis, Bataller Alberola, Ramón, Berdasco Menéndez, María, Bernabeu Morón, Ignacio, Berraondo López, Pedro, Berzigotti, Annalissa, Biagetti, Betina, Bielsa Marsol, Isabel, Blanco Amil, Carla L., Blanco Arévalo, José Luis, Blanco García, Francisco Javier, Blanco Vich, Isabel, Blasco Pelicano, Miquel, Bodro Marimont, Marta, Boronat Guerrero, Susana, Borrego Rabasco, Francisco, Bouza Santiago, Emilio, Brito Zerón, Pilar, Brugada Terradellas, Josep, Brugaletta, Salvatore, Bruix Tudó, Jordi, Bruna Escuer, Jordi, Buján Rivas, Segundo, Fernández de Piérola, Luis Bujanda, Buti Ferret, Maria, Cabellos Mínguez, Carmen, Cahn, Pedro E., Calvet Calvo, Xavier, Calvo Escalona, Rosa, Calvo González, Xavier, Campins Martí, Magda, Campistol Plana, Josep M.ª, Campo Güerri, Elías, Campuzano Larrea, Óscar, Campuzano Uceda, Victoria, Cardellach López, Francesc, Cardoner Alvárez, Narcís, Cardozo Espínola, Celia G., Carmena Rodríguez, Rafael, Carmona Herrera, Francisco, Carracedo Álvarez, Ángel, Carracedo Pérez, Arkaitz, Carreño Serrano, Mar, Carreres Mola, Anna, Casabona i Barbarà, Jordi, Casademont Pou, Jordi, Casanueva Freijo, Felipe F., Casas García, Irma, Cases Amenós, Aleix, Castells Garangou, Antoni, Castelo-Branco, Camil, Castón Osorio, Juanjo, Castro Fornieles, Josefina, Castro Rebollo, Pedro, Catalán Eraso, Beatriz, Caylà Buqueras, Joan, Cervera Álvarez, Carlos, Cervera Segura, Ricard, Chamorro Sánchez, Ángel, Cinta Cid Xutglà, M.ª, Cigudosa García, Juan C., José Coll Rosell, M.ª, Colmenero Arroyo, Jordi, Compta Hirnyj, Yaroslau, Conde Blanco, Estefanía, Corell Almuzara, Alfredo, Cortés Vicente, Elena, Crespo Casal, Manuel, Crespo Conde, Gonzalo, Crespo García, Javier, Cristófol Allué, Ramón, Cubiella Fernández, Joaquín, Cuenca Estrella, Manuel, Dalmases Cleries, Mireia, Dalmau Obrador, Josep, de Frutos Seminario, Fernando, de la Sierra Iserte, Alejandro, de Madaria Pascual, Enrique, del Campo Casanelles, Miguel, Dolores del Olmo García, M.ª, del Río Bueno, Ana, Delgado González, Julio, Diekmann, Fritz, Domínguez Benítez, José Antonio, Domínguez García, Ángela, Domínguez Luengo, Carmen, Ángeles Domínguez Luzón, M.ª, Jesús Domínguez Tordable, M.ª, Dopazo Blázquez, Joaquín, Dueñas Laita, Antonio, Eduardo Durán Rebolledo, Carlos, Duró Pujol, Joan Carles, Echevarría Mayo, Juan Emilio, Eiros Bouza, José M.ª, Ignasi Elizalde Frez, J., Embid López, Cristina, Engel Rocamora, Pablo, de Salamanca Lorente, Rafael Enríquez, España Alonso, Agustín, Espígol-Frigolé, Georgina, Esteller Badosa, Manel, Esteve Comas, María, Esteve Reyner, Jordi, Estruch Riba, Ramón, Feliu Sánchez, Jesús, Fernández Bañares, Fernando, Fernández Fernández, Óscar, Fernández Gómez, Javier, Fernández Llama, Patricia, Fernández Pombo, Antía, Ferrer Monreal, Miguel, Figuerola Pino, Daniel, Fillat Fonts, Cristina, Fonollosa Pla, Vicent, Fontanellas Romà, Antonio, Forcada Vega, Carme, Forner González, Alejandro, Forns Bernhardt, Xavier, Fortuny Guasch, Claudia, Franco de Castro, Agustín, de la Vega, Irene Fuertes, Fullana Rivas, Miquel Àngel, Gaba García, Lydia, Gaig Ventura, Carles, Galarza Delgado, Dionicio Ángel, Galicia Paredes, Miguel, Gállego Culleré, Montserrat, García de Vinuesa, Pastora Gallego, García Lareo, Manuel, García-Moncó, Juan Carlos, García Nieto, Víctor Manuel, García Olivé, Ignasi, Carlos García-Pagán, Juan, García Pavía, Pablo, García Rizo, Clemente, García Sánchez, José Elías, García-Vidal, C., García Villoria, Julia, Garrabou Tornos, Glòria, Garrido Marín, Eduardo, Gascón i Brustenga, Joaquim, Gatell Artigas, José M.ª, Gaya Valls, Anna, Gaztambide Sáenz, Sonia, Genescà Ferrer, Joan, Giavina-Bianchi, Pedro, Gil Rodríguez, Almudena, Giménez Pérez, Montserrat, Giné Soca, Eva, Ginés Gibert, Pere, Goday Arnó, Alberto, Gómez Balaguer, Marcelino, Gómez-Batiste Alentorn, Xavier, Gómez Bueno, Manuel, Gómez Dantés, Héctor, Gomollón García, Fernando, González Argenté, Francesc Xavier, González Juanatey, José Ramón, González Macías, Jesús, González Martín, Julià, Gracia Guillén, Diego Miguel, Grande i Fullana, Iria, Grau Junyent, Josep M.ª, García-Milà, Isabel Graupera, Graus Ribas, Francesc, Guañabens Gay, Nuria, Guasch Casany, Eduard, Gurguí Ferrer, Mercè, Ponce de León, Fernando Gutiérrez, Alexandra Hanzu, Felicia, Hawkins Carranza, Federico, Hernández Boluda, Juan Carlos, Hernández García, Miguel Teodoro, Hernández-Gea, Virginia, Hernández Rodríguez, José, Herrero Santos, José Ignacio, Ibáñez Toda, Lourdes, Iranzo de Riquer, Alejandro, Jiménez Castro, David, Juan Otero, Manuel, Juanola Roura, Xavier, Kalil, Jorge, Khamashta, Munther, Labarca Labarca, Jaime, Laborde García, Amalia, Lamas Oliveira, Cristina, Lanas Arbeloa, Ángel, Landete Rodríguez, Pedro, Larrosa Escartín, Nieves, Lázaro García, Luisa, Lens García, Sabela, Lleó Bisa, Alberto, Carlota Londoño Hurtado, M.ª, Bernaldo de Quirós, Juan Carlos López, López Granados, Eduardo, López Guillermo, Armando, López Meseguer, Manuel, López-Soto, Alfonso, López-Vélez Pérez, Rogelio, Lozano Mejorada, Rebeca, Lozano Sánchez, Francisco. S., Lozano Soto, Francisco, Luján Torné, Manuel, Lumbreras Bermejo, Carlos, Mallolas Masferrer, Josep, Manzanera López, Rafael, Mañá Rey, Juan, Marco Reverté, Francesc, Ángeles Marcos Maeso, M.ª, Marimón Ortiz de Zárate, José M.ª, Mariño Méndez, Zoe, Maroñas Amigo, Olalla, Marrades Sicart, Ramon, de la Iglesia, Jaume Marrugat, José Martí Domènech, M.ª, Martí Mestre, Xavier, Martí Ripoll, Mercè, Cristina Martín Sierra, M.ª, Díaz-Guerra, Guillermo Martínez, Martínez Lavín, Manuel, Martínez Martínez, José Antonio, Martínez Valle, Fernando, Martínez Vea, Alberto, Martínez Yoldi, Miguel Julián, Masana Marín, Lluís, Mascaró Galy, José Manuel, Matas Andreu, Lurdes, Mateo Soria, Lourdes, Victoria Mateos Manteca, M.ª, Maurel Santasusana, Joan, Melero Bermejo, Ignacio, Melero Maseda, Marcelo José, Mensa Pueyo, José, Mensa Vilaró, Anna, Mercé Klein, Jordi, Mestres Alomar, Gaspar, Mezquita Pérez, Laura, Milà Recasens, Montserrat, Milisenda, José, Milone, Jorge H., Miralles Basseda, Ramón, Miralles Hernández, Manuel, Miró Meda, Josep M.ª, Mòdol Deltell, Josep M.ª, Molero Richard, Xavier, Molina Infante, Javier, Molina Molina, María, Molins López-Rodó, Laureano, Mont Girbau, Josep Lluís, Montoro Huguet, Miguel Ángel, Montoya González, María, Mora Porta, Mireia, Morales-Olivas, Francisco, Morales Romero, Blai, Morán Chorro, Indalecio, Morata Ruiz, Laura, Asunción Moreno Camacho, M.ª, Muga Bustamante, Roberto, Muñoz Almagro, Carmen, Muñoz Bermúdez, Rosana, Muñoz Gall, Xavier, Esteban Muñoz García, José, Muñoz García-Paredes, Patricia, Muñoz Gutiérrez, José, Muñoz Mateu, Monserrat, Muñoz Villegas, Álvaro, Murillo Rubio, Óscar, África Muxí Pradas, M.ª, Navarro Acebes, Xavier, Navarro Otano, Judith, Nicolás Arfelis, Josep M.ª, Nolla Solé, Joan Miquel, Norman, Francesca F., Obach Baurier, Víctor, Tomás Obrador Vera, Gregorio, Oliva Dámaso, Elena, Olivé Plana, Montserrat, Oriola Ambrós, Josep, Ortiz Arduan, Alberto, de Lejarazu Leonardo, Raúl Ortiz, Oteo Revuelta, José Antonio, Palau Martínez, Francesc, Pallarés Giner, Román, Palou Rivera, Eduard, Paredes Deiros, Roger, Parramon Puig, Gemma, Pascal Capdevila, Mariona, Pascual Gómez, Eliseo, Pascual Gómez, Julio, Pascual Mateos, Juan Carlos, Pedro-Botet Montoya, Juan, Luisa Pedro-Botet Montoya, M.ª, Pereira Saavedra, Arturo, Pérez Gisbert, Javier, Picado Puig, Albert, Picó Alfonso, Antonio, Pigrau Serrallach, Carlos, Jesús Pinazo Delgado, M.ª, Pintado García, Vicente, Pintor Pérez, Luis, Pintos Morell, Guillem, Piñeiro Sánchez, Daniel José, Piqueras Carrasco, Josep, Plaza Moral, Vicente, López de Briñas, Esteban Poch, Pons-Estel, Bernardo A., Pons-Estel, Guillermo J., Pons Lladó, Guillem, Porcel Pérez, José Manuel, Pozo-Rosich, Patricia, Praga Terente, Manuel, de Medicina, Catedrático, de Medicina, Facultad, Complutense de Madrid, Universidad, Prat Aparicio, Aleix, Prat Marín, Andrés, Puig Domingo, Manuel, Pulido Mestre, Marta, Pumarola Suñé, Tomás, Puras Mallagray, Enrique, Queiro Silva, Rubén, Quintana Porras, Luis F., Quintero Carrión, Enrique, Adrián Rabinovich, Gabriel, Rabionet Janssen, Raquel, Ramos Casals, Manuel, Antoni Ramos Quiroga, J., Raya Sánchez, José M.ª, José Rego Castro, M.ª, Reig Monzón, María, Reig Torras, Óscar, Reverter Calatayud, Joan Carles, Reverter Calatayud, Jorge Luis, Reverter Segura, Enric, Reyes Cabanillas, Roxana, Reyes Moreno, José, Riambau Alonso, Vicenç, Ribera Casado, José Manuel, Ribera Santasusana, Josep M.ª, Ribes Rubió, Antonia, Ricart Gómez, Elena, Rico Caballero, Verónica, Robert Boter, Neus, Roca Lecumberri, Alba, Roca Luque, Ivo, Roca Torrent, Josep, Roche Rebollo, Enric, Gonzalo de Liria, Carlos Rodrigo, Rodríguez Baño, Jesús, de Castro, Felipe Rodríguez, Rodríguez Pérez, José Carlos, Rodríguez-Revenga, Laia, Rodríguez-Valero, Natalia, Roé Crespo, Esther, Rojas García, Ricardo, Romero-Gómez, Manuel, Romero Romero, Beatriz, Ros Cerro, Cristina, Rosiñol Dachs, Laura, Rovira Cañellas, Àlex, Rozman, Ciril, Rozman Jurado, María, Teresa Ruiz Cantero, M.ª, Ruiz Manzano, Juan, Ruiz Moreno, Javier, Ortiz de Arizabaleta, Estíbaliz Ruiz, Sabaté Tenas, Manel, Sáiz Hinarejos, Albert, Sala Sanjaume, Joan, Salgado García, Emilio José, Salinas Vert, Isabel, del Valle Díaz, Raquel Sánchez, Sánchez González, Marcelo, Sanmartí Sala, Raimon, Sanz Alonso, Miguel Ángel, Saperas Franch, Esteban, Saurí Nadal, Tamara, Schwarzstein, Diego, Segovia Cubero, Javier, Segura Egea, Antònia, Segura Porta, Ferran, Sellarés Torres, Jacobo, Selva O’Callaghan, Albert, Serra Majem, Lluís, Serra Pueyo, Jordi, Serrano Gimaré, Mercedes, Sibila Vidal, Oriol, Sierra Gil, Jorge, Sierra Romero, Gustavo Adolfo, Sionis, Alessandro, Sitges Carreño, Marta, Solà Vergès, Elsa, Solana Lara, Rafael, Solans Laqué, Roser, Gloria Soldevila Melgarejo, M.ª, Soler Porcar, Néstor, Sopena Galindo, Nieves, Soriano Viladomiu, Álex, Soy Muner, Dolores, Suárez Fernández, Ricardo, Suela Rubio, Javier, Targarona Soler, Eduardo M.ª, Tolosa Sarró, Eduardo, Tornero Molina, Jesús, Tornos Mas, Pilar, Torra Balcells, Roser, Torregrosa Prats, José Vicente, Torres Hortal, Montserrat, Torres Martí, Antonio, Torres Ramírez, Armando, Trilla García, Antoni, Trujillo Reyes, Juan Carlos, Tuca Rodríguez, Albert, Tudela Hita, Pere, Urbano Ispizua, Álvaro, Urra Nuin, Xabier, de Diego, Agustín Urrutia, Valero Santiago, Antonio L., Vallderiola Serra, Francesc, Valle Velasco, Laura, Vallés Angulo, Sara, Vaquero Raya, Eva, Vargas Núñez, Juan Antonio, Varsavsky, Mariela, Vázquez Martínez, Clotilde, San Miguel, Federico Vázquez, Vergara Gómez, Andrea, Vicente García, Vicente, Vidal Bermúdez, José Ernesto, Vidal Losada, María, Vidal-Puig, Antonio, Vieta Pascual, Eduard, Vila Estapé, Jordi, Vilabona Artero, Carles, Vilardell Tarrés, Miquel, Vilaseca González, Isabel, Vilella i Morató, Anna, Villà Freixa, Salvador, Villamor Casas, Neus, Vives Corrons, Joan Lluís, Vives Pi, Marta, Volberg Vincent, Verónica Inés, Vollmer Torrubiano, Ivan, Webb Youdale, Susan, Yagüe Ribes, Jordi, and Yugueros Castellnou, Xavier

Published
2024
Full Text
View/download PDF

50. Chilblain-like lesions and COVID-19 infection: A prospective observational study at Spain's ground zero

Author

Feito-Rodríguez, Marta, Mayor-Ibarguren, Ander, Cámara-Hijón, Carmen, Montero-Vega, Dolores, Servera-Negre, Guillermo, Ruiz-Bravo, Elena, Nozal, Pilar, Rodríguez-Peralto, José Luis, Enguita, Ana Belén, Bravo-Gallego, Luz Yadira, Granados-Fernández, Marí, Fernández-Alcalde, Celia, Fernández-Heredero, Álvaro, Alonso-Riaño, Marina, Jiménez-Yuste, Víctor, Nuño-González, Almudena, De Lucas-Laguna, Raúl, López-Granados, Eduardo, and Herranz-Pinto, Pedro

Published
2021
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results