Search

Your search keyword '"López-Varea, Ana"' showing total 21 results
Start Over Author "López-Varea, Ana"
21 results on '"López-Varea, Ana"'

5. Genome-wide phenotypic RNAi screen in the Drosophila wing: global parameters

Author

López-Varea, Ana, primary, Ostalé, Cristina M, additional, Vega-Cuesta, Patricia, additional, Ruiz-Gómez, Ana, additional, Organista, María F, additional, Martín, Mercedes, additional, Hevia, Covadonga F, additional, Molnar, Cristina, additional, de Celis, Jesús, additional, Culi, Joaquim, additional, Esteban, Nuria, additional, and de Celis, Jose F, additional

Published
2021
Full Text
View/download PDF

7. Genome-wide phenotypic RNAi screen in the Drosophila wing: phenotypic description of functional classes

Author

López-Varea, Ana, primary, Vega-Cuesta, Patricia, additional, Ruiz-Gómez, Ana, additional, Ostalé, Cristina M, additional, Molnar, Cristina, additional, Hevia, Covadonga F, additional, Martín, Mercedes, additional, Organista, Maria F, additional, de Celis, Jesus, additional, Culí, Joaquín, additional, Esteban, Nuria, additional, and de Celis, Jose F, additional

Published
2021
Full Text
View/download PDF

11. Conserved cross-interactions in Drosophila and Xenopus between Ras/MAPK signaling and the dual-specificity phosphatase MKP3

Author

Ruiz-Gómez, Ana, López-Varea, Ana, Molnar, Cristina, Calle-Mustienes, Elisa de la, Ruiz-Gómez, Mar, Gómez-Skarmeta, José Luis, Celis, José F. de, Ministerio de Ciencia y Tecnología (España), Ministerio de Economía y Competitividad (España), and Fundación Ramón Areces

Subjects
Vein formation, Neural development, Negative feedback, Xenopus, Drosophila, MAPK, Cell signalling
Abstract

9 páginas, 7 figuras., The extracellular signal-regulated kinase (ERK) is a key transducer of the epidermal growth factor receptor (EGFR) and fibroblast growth factor receptor (FGFR) signaling pathways, and its function is required in multiple processes during animal development. The activity of ERK depends on the phosphorylation state of conserved threonine and tyrosine residues, and this state is regulated by different kinases and phosphatases. A family of phosphatases with specificity toward both threonine and tyrosine residues in ERK (dual-specificity phosphatases) play a conserved role in its dephosphorylation and consequent inactivation. Here, we characterize the function of the dual-specificity phosphatase MKP3 in Drosophila EGFR and Xenopus FGFR signaling. The function of MKP3 is required during Drosophila wing vein formation and Xenopus anteroposterior neural patterning. We find that the expression of the MKP3 gene is localized in places of high EGFR and FGFR signaling. Furthermore, this restricted expression depends on ERK function both in Drosophila and Xenopus, suggesting that MKP3 constitutes a conserved negative feedback loop on the activity of the Ras/ERK signaling pathway., Funded by Ministry of Science and Technology. Grant Numbers: BCM2000-1191, GEN2001-4846-C0501, BMC2001-2122. Dirección General de Investigación Cientófica y Técnica. Fundación Ramón Areces.

Published
2005

20. Conserved cross‐interactions in Drosophila and Xenopus between Ras/MAPK signaling and the dual‐specificity phosphatase MKP3

Author

Gómez, Ana Ruiz, López‐Varea, Ana, Molnar, Cristina, de la Calle‐Mustienes, Elisa, Ruiz‐Gómez, Mar, Gómez‐Skarmeta, José Luis, and de Celis, Jose F.

Abstract

The extracellular signal‐regulated kinase (ERK) is a key transducer of the epidermal growth factor receptor (EGFR) and fibroblast growth factor receptor (FGFR) signaling pathways, and its function is required in multiple processes during animal development. The activity of ERK depends on the phosphorylation state of conserved threonine and tyrosine residues, and this state is regulated by different kinases and phosphatases. A family of phosphatases with specificity toward both threonine and tyrosine residues in ERK (dual‐specificity phosphatases) play a conserved role in its dephosphorylation and consequent inactivation. Here, we characterize the function of the dual‐specificity phosphatase MKP3 in Drosophila EGFR and Xenopus FGFR signaling. The function of MKP3 is required during Drosophila wing vein formation and Xenopus anteroposterior neural patterning. We find that the expression of the MKP3 gene is localized in places of high EGFR and FGFR signaling. Furthermore, this restricted expression depends on ERK function both in Drosophila and Xenopus, suggesting that MKP3 constitutes a conserved negative feedback loop on the activity of the Ras/ERK signaling pathway. Developmental Dynamics 232:695–708, 2005. © 2005 Wiley‐Liss, Inc.

Published
2005
Full Text
View/download PDF

21. Drosophila laminins act as key regulators of basement membrane assembly and morphogenesis.

Author

Urbano, Jose M., Torgler, Catherine N., Molnar, Cristina, Tepass, Ulrich, López-Varea, Ana, Brown, Nicholas H., de Celis, Jose F., and Martín-Bermudo, Maria D.

Subjects
DROSOPHILA, MORPHOGENESIS, BIOLOGICAL membranes, GASTRULATION, GENOMES, GENETIC mutation, EMBRYOLOGY
Abstract

Laminins are heterotrimeric molecules found in all basement membranes. In mammals, they have been involved in diverse developmental processes, from gastrulation to tissue maintenance. The Drosophila genome encodes two laminin α chains, one β and one γ, which form two distinct laminin trimers. So far, only mutations affecting one or other trimer have been analysed. In order to study embryonic development in the complete absence of laminins, we mutated the gene encoding the sole laminin β chain in Drosophila, LanB1, so that no trimers can be made. We show that LanB1 mutant embryos develop until the end of embryogenesis. Electron microscopy analysis of mutant embryos reveals that the basement membranes are absent and the remaining extracellular material appears disorganised and diffuse. Accordingly, abnormal accumulation of major basement membrane components, such as Collagen IV and Perlecan, is observed in mutant tissues. In addition, we show that elimination of LanB1 prevents the normal morphogenesis of most organs and tissues, including the gut, trachea, muscles and nervous system. In spite of the above structural roles for laminins, our results unravel novel functions in cell adhesion, migration and rearrangement. We propose that while an early function of laminins in gastrulation is not conserved in Drosophila and mammals, their function in basement membrane assembly and organogenesis seems to be maintained throughout evolution. [ABSTRACT FROM AUTHOR]

Published
2009
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results