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Three scholars share their pandemic experiences, weaving narrative and poetry in an anti-anti-narrative of illness, death, stress, and fear. This is a tale of relentless living at a distance—of the drip, drip, drip, drip, drip, minute by minute, year after year, endless cascade of unresolved anxiety and the shared cortisol spike so many of us have endured slogging through the interminable pandemic in a death-indifferent culture. more...

As information ages, it may become less accurate, resulting in increased uncertainty for decision makers. For example, chemical alarm cues (AC) are a source of public information about a nearby predator attack, and these cues can become spatially inaccurate through time. These cues can also degrade quickly under natural conditions, and cue receivers are sensitive to such degradation. Although numerous studies have documented predator-recognition learning from fresh AC, no studies have explored learning from aged AC and whether the uncertainty associated with this older information contributes to shortening the retention of learned responses (i.e. the ‘memory window’). Here, we found that wood frog tadpoles, Lithobates sylvaticus , learned to recognize a novel odour as a predator when paired with AC aged under natural conditions for up to 1 h. However, only tadpoles conditioned with fresh AC were found to retain this learned response when tested 9 days after conditioning. These results support the hypothesis that the memory window is shortened by the uncertainty associated with older information, preventing the long-term costs of a learned association that was based on potentially outdated information. more...

Animals can reduce their uncertainty of predation risk by gathering new information via exploration behaviour. However, a decision to explore may also be costly due to increased predator exposure. Here, we found contextual effects of predation risk on the exploratory activity of Trinidadian guppies Poecilia reticulata in a novel environment. First, guppies were exposed to a 3-day period of either high or low background predation risk in the form of repeated exposure to either injured conspecific cues (i.e. alarm cues) or control water, respectively. A day later, guppies were moved into a testing arena with limited visual information due to structural barriers and were then presented with an acute chemical stimulus, either alarm cues (a known and reliable indicator of risk), a novel odour (an ambiguous cue), or control water. In the presence of control water, guppies from high and low background risk showed a similar willingness to explore the arena. However, high-risk individuals significantly reduced their spatial evenness, although not their movement latency, in the presence of both the alarm and novel cues. When these high-risk individuals were a member of a shoal, they became willing to explore the environment more evenly in the presence of alarm cues while remaining cautious toward the novel cue, indicating an effect of the greater uncertainty associated with the novel cue. In contrast, low-risk guppies showed a willingness to explore the arena regardless of acute threat or social context. Such contextual effects of background risk and social context highlight the complexity of exploratory decisions when uncertain. more...

This case details the presentation and surgical management of a 15-year-old male patient with multiple endocrine neoplasia syndrome type 1 (MEN1) who required distal pancreatectomy for multiple nonfunctional pancreatic tumors. An intraoperative ultrasound was utilized to allow for proper location of the distal pancreatectomy, as well as visualization of the splenic vessel relationships and to ensure all lesions were contained within the specimen. Pathology demonstrated 5 well-differentiated neuroendocrine tumors with no evidence of malignancy. This case utilized innovative technology and a multidisciplinary approach in a challenging case to achieve a safe minimally invasive resection. The use of ultrasound intraoperatively provided confidence that all lesions had been identified, as well as demonstration of safe planes separate from the nearby vasculature. more...

Many species possess damage-released chemical alarm cues that function in alerting nearby individuals to a predator attack. One hypothesis for the evolution and/or maintenance of such cues is the Predator Attraction Hypothesis, where predators, rather than prey, are the "intended" recipients of these cues. If a predator attack attracts additional predators, these secondary predators might interfere with the predation event, providing the prey with a better chance to escape. In this study, we conducted two experiments to explore this hypothesis in an amphibian predator/prey system. In Experiment 1, we found that tiger salamanders (Ambystoma mavortium) showed a foraging attraction to chemical cues from wood frog (Lithobates sylvaticus) tadpoles. Salamanders that were experienced with tadpole prey, in particular, were strongly attracted to tadpole alarm cues. In Experiment 2, we observed experimental encounters between a tadpole and either one or two salamanders. The presence of the second predator caused salamanders to increase attack speed at the cost of decreased attack accuracy (i.e., increasing the probability that the tadpole would escape attacks). We also found that the mere presence of visual and chemical cues from a second predator did not affect this speed/accuracy trade-off but did cause enough of a distraction to increase tadpole survival. Thus, our findings are consistent with the Predator Attraction Hypothesis for the evolution and/or maintenance of alarm cues. more...

When faced with uncertainty, animals can benefit from using multiple sources of information in order to make an optimal decision. However, information sources (e.g., social and personal cues) may conflict, while also varying in acquisition cost and reliability. Here, we assessed behavioral decisions of Trinidadian guppies (Poecilia reticulata), in situ, when presented with conflicting social and personal information about predation risk. We positioned foraging arenas within high- and low-predation streams, where guppies were exposed to a personal cue in the form of conspecific alarm cues (a known indicator of risk), a novel cue, or a control. At the same time, a conspecific shoal (a social safety cue) was either present or absent. When social safety was absent, guppies in both populations showed typical avoidance responses towards alarm cues, and high-predation guppies showed their typical avoidance of novel cues (i.e., neophobia). However, the presence of social safety cues was persuasive, overriding the neophobia of high-predation guppies and emboldening low-predation guppies to ignore alarm cues. Our experiment is one of the first to empirically assess the use of safety and risk cues in prey and suggests a threshold level of ambient risk which dictates the use of conflicting social and personal information. more...

Lateralization of cognitive functions impacts many behaviours related to fitness and, in most species, varies greatly among individuals. Laboratory and field studies have suggested that within-species variation in lateralization is partly due to phenotypic plasticity. For example, in fish, prey that have experienced predation risk during early ontogeny develop highly lateralized phenotypes, and this lateralization often favours prey in evading predators. In contexts other than predation, plasticity of lateralization has also been reported for adult fish. Therefore, we asked whether adult fathead minnows, Pimephales promelas, exposed to high predation risk would also show plasticity linked to increase lateralization. We exposed minnows to conspecific alarm cues for up to 8 days to simulate predation risk and tested their lateralization with a standard detour test. The treatment affected lateralization but in an unexpected direction: Individuals exposed to high predation risk showed lower lateralization scores compared to control fish. In addition, fish within groups exposed to risk reduced the variability in their directionality of lateralization; that is, they showed a similar turning preference in the detour task. Our study suggests that lateralization can vary in response to predation risk in adult fish. (PsycInfo Database Record (c) 2020 APA, all rights reserved). more...

Predation fear is a unifying theme across vertebrate taxa. Here, we explored how the frequency and duration of predation risk affects postrisk fear behavior in Trinidadian guppies. We first exposed individuals to visual cues of potential predators for 3 days, either frequently (6×/day) or infrequently (1×/day). Each exposure lasted for either a relatively brief (5 min) or long (30 min) duration, whereas a control group consisted of no risk exposures. One day later, we quantified guppy behavior. All background risk treatments induced a fear response toward a novel odor (i.e., neophobia), and individuals previously exposed to frequent bouts of brief risk showed elevated baseline fear. Although neophobic responses were initially similar across risk treatments (1 day later), retention of this response differed. After 8 days, only individuals previously exposed to brief bouts of risk (both frequent and infrequent) maintained neophobic responses, whereas their initially higher level of baseline fear remained elevated but was no longer significantly different from the control. These results increase our understanding of temporal factors that affect the intensity and retention of fear that persists after risk exposure, which may have applications across vertebrates in relation to problems with fearful phenotypes. more...

Even though mollusks' capacity to repair shell damage is usually studied in response to a single event, their shells have to defend them against predatory and environmental threats throughout their potentially multi-decadal life. We measured whether and how mollusks respond to chronic mechanical stress. Once a week for 7 months, we compressed whole live California mussels (Mytilus californianus) for 15 cycles at ∼55% of their predicted one-time breaking force, a treatment known to cause fatigue damage in shells. We found mussels repaired their shells. Shells of experimentally stressed mussels were just as strong at the end of the experiment as those of control mussels that had not been experimentally loaded, and they were more heavily patched internally. Additionally, stressed shells differed in morphology; they were heavier and thicker at the end of the experiment than control shells but they had increased less in width, resulting in a flatter, less domed shape. Finally, the chronic mechanical stress and repair came at a cost, with stressed mussels having higher mortality and less soft tissue than the control group. Although associated with significant cost, mussels' ability to maintain repair in response to ongoing mechanical stress may be vital to their survival in harsh and predator-filled environments. more...

Background The Children's Oncology Group recommends upfront resection of Wilms tumor (WT), however, unique scenarios warrant neoadjuvant chemotherapy and delayed resection. We hypothesized that in the context of neoadjuvant chemotherapy, minimally invasive surgery (MIS) to resect WT achieves equivalent oncologic fidelity and better maintains therapy schedules. Methods A retrospective analysis of WT treated between 2010-2021 at a free-standing children's hospital was performed. Patient and disease specific characteristics were collected, and pre-resection tumor volumes (TV) were calculated. Impact of MIS or open resection on oncologic fidelity and time to resume chemotherapy was analyzed. Results For the study period, 62 patients were treated for 65 WT, and 14 patients (22.6%) received neoadjuvant chemotherapy to treat 17 WT (26.2%): 7 Stage I (all predisposition syndromes), 2 stage III, 7 stage IV, and 1 stage V (bilateral). MIS was utilized to resect 6 WT from 5 patients. For partial nephrectomy, pre-resection TV was 0.38 ml if MIS and 10.38 ml if open ( P = .025). For radical nephrectomy, pre-resection TV was 31.58 ml if MIS and 175.00 ml if open ( P = .101). No significant differences between surgical approach were detected regarding pathologic variables or survival. Epidural use was significantly greater with open procedures ( P = .001). Length of stay was 2.00 days after MIS compared to 6.00 for open resection ( P = .004). Time to resume chemotherapy was 7.00 days after MIS versus 27.00 for open ( P = .004). Conclusion After neoadjuvant chemotherapy for WT, MIS partial and radical nephrectomies achieved equivalent oncologic fidelity, reduced epidural use and post-operative stays, and better maintained adjuvant therapy timelines when compared to open resections. more...

Hard external armors have to defend against a lifetime of threats yet are traditionally understood by their ability to withstand a single attack. Survival of bivalve mollusks thus can depend on the ability to repair shell damage between encounters. We studied the capacity for repair in the intertidal mussel Mytilus californianus by compressing live mussels for 15 cycles at ∼79% of their predicted strength (critically fracturing 46% of shells), then allowing the survivors 0, 1, 2 or 4 weeks to repair. Immediately after fatigue loading, mussel shells were 20% weaker than control shells that had not experienced repetitive loading. However, mussels restored full shell strength within 1 week, and after 4 weeks shells that had experienced greater fatiguing forces were stronger than those repetitively loaded at lower forces. Microscopy supported the hypothesis that crack propagation is a mechanism of fatigue-caused weakening. However, the mechanism of repair was only partially explained, as epifluorescence microscopy of calcein staining for shell deposition showed that only half of the mussels that experienced repetitive loading had initiated direct repair via shell growth around fractures. Our findings document repair weeks to months faster than demonstrated in other mollusks. This rapid repair may be important for the mussels’ success contending with predatory and environmental threats in the harsh environment of wave-swept rocky coasts, allowing them to address non-critical but weakening damage and to initiate plastic changes to shell strength. We highlight the significant insight gained by studying biological armors not as static structures but, instead, as dynamic systems that accumulate, repair and respond to damage., Highlighted Article: Mussels repair shell damage caused by fatigue within one week and further strengthen shells within one month. Bivalve shells are a dynamic armor, responsive to accumulating weakening damage. more...

In the growing skeleton, angiogenesis is intimately coupled with osteogenesis. Chronic, high doses of glucocorticoids (GCs) are associated with decreased bone vasculature and induce osteoporosis and growth failure. The mechanism of GC-suppression of angiogenesis and relationship to osteoporosis and growth retardation remains largely unknown. Type H vessels, which are regulated by preosteoclast (POC) platelet-derived growth factor-BB (PDGF-BB), are specifically coupled with bone formation and development. We determined the effect of GCs on POC synthesis of PDGF-BB in relation to type H vessel formation, bone mass, and bone growth in the distal femur of 2-week-old young mice receiving prednisolone or vehicle for 2, 4, or 6 weeks. After 2 weeks of prednisolone, the number of POCs were unchanged while POC synthesis of PDGF-BB was reduced. Longer treatment with prednisolone reduced POCs numbers and PDGF-BB. These changes were associated with a reduction in type H vessels, bone formation rate, bone mass, and bone length at each time point. In vitro, excessive concentrations of prednisolone (10-6 M) resulted in decreased PDGF-BB concentration and POC numbers. Conditioned medium from POC cultures treated with control concentration of prednisolone (10-7 M) or recombinant PDGF-BB stimulated endothelial tube formation, whereas conditioned medium from control concentration of prednisolone-treated POC cultures neutralized by PDGF-BB antibody or excessive prednisolone inhibited endothelial tube formation. Administration of excessive prednisolone attenuated the P65 subunit of nuclear factor kappa B (NF-κB) binding to the Pdgfb promoter, resulting in lower Pdgfb transcription. Co-treatment with excessive prednisolone and the glucocorticoid receptor (GR) antagonist (RU486), GR siRNA, or TNFα rescued NF-κB binding to the Pdgfb promoter and endothelial tube formation. These results indicate that PDGF-BB synthesis in POCs is suppressed by GCs through transrepression of GR/NF-κB, thus inhibiting type H vessel formation and associated osteoporosis and growth failure. © 2020 American Society for Bone and Mineral Research. more...

In the mammalian skeletal system, osteogenesis and angiogenesis are intimately linked during bone growth and regeneration in bone modeling and during bone homeostasis in bone remodeling. Recent studies have expanded our knowledge about the molecular and cellular mechanisms responsible for coupling angiogenesis and bone formation. Type H vessels, termed such because of high expression of Endomucin (Emcn) and CD31, have recently been identified and have the ability to induce bone formation. Factors including platelet-derived growth factor type BB (PDGF-BB), slit guidance ligand 3 (SLIT3), hypoxia-inducible factor 1-alpha (HIF-1α), Notch, and vascular endothelial growth factor (VEGF) are involved in the coupling of angiogenesis and osteogenesis. This review summarizes the current understanding of signaling pathways that regulate type H vessels and how type H vessels modulate osteogenesis. Further studies dissecting the regulation and function of type H vessels will provide new insights into the role of bone vasculature in the metabolism of the skeleton. We also discuss considerations for therapeutic approaches targeting type H vessels to promote fracture healing, prevent pathological bone loss, osteonecrosis, osteoarthritis, and bone metastases. more...

STUDY OBJECTIVE: Newly diagnosed pediatric patients with type 1 diabetes mellitus (T1D) can be underweight, overweight, or normal weight at presentation. Study objectives were to determine if, across weight categories, admission body weight (ABW)-based initial insulin glargine dosing resulted in similar fasting blood glucose responses on day of discharge, how initial ABW-based doses differed from doses at outpatient follow-up, and whether an ideal body weight (IBW) would provide a better estimate of body weight after discharge. DESIGN: Retrospective chart review. SETTING: Urban tertiary academic medical center. PATIENTS: Eighty-one pediatric patients newly diagnosed with T1D who started therapy with subcutaneous insulin glargine between October 2014 and October 2016; patients were categorized by weight using body mass index (BMI) percentiles (underweight, normal weight, overweight/obese). MEASUREMENTS AND MAIN RESULTS: Data on patient parameters from hospitalization and outpatient physician follow-up were collected. The McLaren, Moore, and BMI IBW methods were used to calculate IBW for each patient; these IBWs were compared with weights at outpatient follow-up. Initial insulin glargine doses were similar among all weight groups: median (range) 0.299 (0.227–0.4), 0.297 (0.204–0.421), and 0.291 (0.194–0.394) units/kg/dose, respectively, for the underweight, normal weight, overweight/obese groups. No significant differences in discharge fasting glucose level or insulin glargine dose change from admission to first outpatient follow-up visit were noted. Underweight patients gained significantly more weight within 60 days after discharge compared with normal and overweight/obese patients, (median 16.3% vs 7.7% and 5.7%, respectively, p=0.002), aligning closest with the McLaren IBW. ABW was the best estimate of weight at outpatient follow-up in the overweight/obese patient group. CONCLUSION: For children who presented underweight, the McLaren IBW method was the best predictor of outpatient dose and body weight, whereas ABW was the best estimate in overweight and obese patients. Further investigation of the role of IBW or adjusted body weight–based dosing methods in underweight pediatric patients with T1D may assist in optimal dosing. more...

There are numerous examples of species introductions that have caused declines in native populations. In many cases, exotic species are predators of native prey which do not respond correctly to these new and often much different threats. Amphibians, as a group, have been strongly affected by introductions of fish and other aquatic predators such as crayfish. Our goal in this study was to explore the potential impacts of exotic crayfish on the behaviour, morphology, and survival of naive wood frog tadpoles, Lithobates sylvaticus. In mesocosms, groups of tadpoles were exposed to either a native predator (larval beetle or dragonfly nymph) or an exotic crayfish, Orconectes virilis. Tadpoles were the largest following exposure to dragonflies, indicating that dragonflies were selecting smaller tadpoles. Vertical space use of tadpoles was highest in the presence of crayfish, suggesting that tadpoles were learning to avoid crayfish in the benthos. Mortality was highest in the presence of beetles and lowest with crayfish, and hence in isolation, exotic crayfish were poorer predators of wood frog tadpoles. However, half way through the experiment, we replaced each predator with a new predator of either the same species or a different species to assess how the impact of the new predator was affected by experience with the first predator. When crayfish were added following beetles, the mortality due to crayfish increased significantly, possibly due to differences in predator space use and foraging mode. more...

Chemical information has an important role in the sensory ecology of aquatic species. For aquatic prey, chemical cues are a vital source of information related to predator avoidance and risk assessment. For instance, alarm cues are released by prey that have been injured by predators. In addition to providing accurate information about current risk, repeated exposure to alarm cues can elicit a fear response to novel stimuli (neophobia) in prey. Another source of chemical information is disturbance cues, released by prey that have been disturbed or harassed (but not injured) by a predator. While disturbance cues have received much less attention than alarm cues, they appear to be useful as an early warning signal of predation risk and have the potential to be used as a priming cue for learning. In this study, we used wood frog (Lithobates sylvaticus) tadpoles to test whether repeated exposure to disturbance cues during the embryonic stage can induce neophobic behaviour. Three weeks following repeated exposure to disturbance cues, tadpoles showed reduced activity when exposed to a novel odour, but they no longer displayed an antipredator response to disturbance cues. In a second experiment, we found that tadpoles failed to learn that a novel odour was dangerous following a pairing with disturbance cues, whereas alarm cues facilitated such learning. Our results add to the growing body of information about disturbance cues and provide evidence of their function as an embryonic risk cue but not as an associative learning cue. more...

Animals can reduce their uncertainty of predation risk by gathering new information via exploration behaviour. However, a decision to explore may also be costly due to increased predator exposure. Here, we found contextual effects of predation risk on the exploratory activity of Trinidadian guppies Poecilia reticulata in a novel environment. First, guppies were exposed to a 3-day period of either high or low background predation risk in the form of repeated exposure to either injured conspecific cues (i.e. alarm cues) or control water, respectively. A day later, guppies were moved into a testing arena with limited visual information due to structural barriers and were then presented with an acute chemical stimulus, either alarm cues (a known and reliable indicator of risk), a novel odour (an ambiguous cue), or control water. In the presence of control water, guppies from high and low background risk showed a similar willingness to explore the arena. However, high-risk individuals significantly reduced their spatial evenness, although not their movement latency, in the presence of both the alarm and novel cues. When these high-risk individuals were a member of a shoal, they became willing to explore the environment more evenly in the presence of alarm cues while remaining cautious toward the novel cue, indicating an effect of the greater uncertainty associated with the novel cue. In contrast, low-risk guppies showed a willingness to explore the arena regardless of acute threat or social context. Such contextual effects of background risk and social context highlight the complexity of exploratory decisions when uncertain. more...