1. Identification, antioxidant and cytotoxic potentials of casticin in Vitex agnus-castus fruit from different geographical regions of Turkey
- Author
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Günay Sariyar, Turgut Taşkın, Esra Eroglu Ozkan, Afife Mat, Gizem Gulsoy Toplan, Mahmoud Abudayyak, Toplan, Gizem Gulsoy, Ozkan, Esra Eroglu, Taskin, Turgut, Abudayyak, Mahmoud, Mat, Afife, and Sariyar, Gunay
- Subjects
Antioxidant ,biology ,Traditional medicine ,Cytotoxic activity ,DPPH ,medicine.medical_treatment ,Vitex ,Pharmaceutical Science ,Casticin ,biology.organism_classification ,Vitex agnus-castus ,APOPTOSIS ,HeLa ,chemistry.chemical_compound ,Anticancer ,chemistry ,L. FRUITS ,Pepper ,CELLS ,medicine ,Pharmacology (medical) ,Gallic acid - Abstract
Purpose: To evaluate the antioxidant and cytotoxic effects, and casticin contents of Vitex agnus-castus (VAC) fruit extract from five geographical regions of Turkey.Methods: Casticin determination in VAC fruit extracts was performed using HPLC-DAD method. The method was validated for linearity, sensitivity, selectivity, accuracy and precision. The methanol extracts of the VAC fruits were analyzed for antioxidant effects using 2,2-diphenyl-1-picrylhydrazyl (DPPH•) radical scavenging capacity, metal-chelation capacity assay, radical cation scavenging capacity (ABTS•+), and total phenolic content. The cytotoxic potential of the extracts against NRK-52E and HeLa cells were determined using MTT and LDH assays.Results: The casticin content of the extracts ranged from 0.048 and 0.152 %. Total phenol content was in the range of 36.67 ± 0.7 to 74.20 ± 1.02 mg gallic acid equivalent (GAE)/100 mg of extracts. High-tomoderate antioxidant properties were observed in the extracts. Cytotoxicity data demonstrated that all extracts showed high cytotoxic effects on HeLa cell line when compared to the NRK-52E cell line (p < 0.05).Conclusion: These results suggest that VAC fruits (Monk’s pepper fruits) from Aegean sea coasts have a potential for use in the preparation of phytopharmaceutical products. Keywords: Vitex agnus-castus, Casticin, Antioxidant, Cytotoxic activity, Anticancer
- Published
- 2020