Author: "L. Gaillard" - Searchworks@Jio Institute Digital Library Search Results

Your search keyword '"L. Gaillard"' showing total 593 results

Start Over Author "L. Gaillard"

593 results on '"L. Gaillard"'

1. ERN CRANIO patient coverage of craniosynostosis in Europe

Author: O. Spivack, L. Gaillard, and ERN CRANIO hospital representatives
Subjects: Craniosynostosis, Prevalence, European Reference Network, ERN, ERN CRANIO, Medicine
Abstract: Abstract Background Against the backdrop of the European Directive on patients’ rights in cross-border healthcare, 24 European Reference Networks (ERNs) were launched in 2017. ERNs are networks of specialised hospitals working together to support patients with rare and/or complex diseases. ERN CRANIO is the ERN for craniofacial anomalies and ear, nose and throat disorders. The aim of this study was to explore ERN CRANIO’s patient coverage of craniosynostosis. Methods ERN CRANIO members and applicants were asked to retrospectively report the number of ‘new craniosynostosis patients’ (isolated and syndromic) seen in 2017. The number of live births per country in 2017 was retrieved from EUROSTAT, the EU’s statistical office. The number of new patients reported per country and the number of live births were used to generate country-specific prevalence figures per 10,000 live births. These figures were compared to expected prevalence ranges for craniosynostosis, and syndromic craniosynostosis specifically, defined by recent European studies. The percentage of syndromic craniosynostosis cases per country was also compared to the expected percentage range. Results Based on previous studies, the expected prevalence ranges for craniosynostosis and syndromic craniosynostosis specifically were respectively defined as 4.4–7.2 and 0.9–1.6 patients/10,000 live births. For craniosynostosis (‘total’; isolated + syndromic), 'new patient' data from the UK and Finland generated prevalence figures within the expected range, and those in France, Spain, Italy, Portugal and Germany are lower than expected. However, when including applicant data, the prevalence figures for France, Spain and Italy become in range. Data from the Netherlands and Sweden generated higher prevalence figures than expected. For France, Finland, Italy and Sweden, there is inconsistency between patient coverage of ‘total’ and syndromic patients. For France, Germany, Finland and Italy, the percentage of syndromic craniosynostosis was lower than the expected range. Conclusion ERN CRANIO’s coverage of craniosynostosis varies across Europe. Results may be explained by data collection methods, genetic testing policies and/or national healthcare systems. With centre caseload a driving force for quality, additional ERN membership calls may not necessarily ensure sufficient patient coverage for countries with decentralised healthcare systems. Liaison with national health ministries should be encouraged to optimise patient coverage.
Published: 2022
Full Text: View/download PDF

2. Long-term disease-free survival with chemotherapy and pembrolizumab in a patient with unmeasurable, advanced stage dedifferentiated endometrial carcinoma

Author: Joy M. Davis, Tullia Rushton, Felicity Nsiah, Rebecca L. Stone, Anna L. Beavis, Stéphanie L. Gaillard, Alice Dobi, and Amanda N. Fader
Subjects: Dedifferentiated endometrial carcinoma, Pembrolizumab, Mismatch repair deficient, Gynecology and obstetrics, RG1-991, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract: Dedifferentiated endometrial carcinoma is a rare, highly aggressive subtype of endometrial cancer associated with poor survival outcomes. Current guidelines recommend treatment of advanced-stage disease with surgical staging or cytoreduction and platinum/taxane-based chemotherapy. Despite these approaches, the achievement of long-term remission or prolonged survival is challenging. Recent Phase III studies demonstrate that the addition of PD-1 inhibitors to standard chemotherapy significantly improves progression-free survival in patients with measurable, mismatch repair deficient (dMMR) and proficient (pMMR) advanced-stage or recurrent endometrial carcinoma. However, the role of PD-1 blockade in the treatment of undifferentiated and dedifferentiated endometrial carcinoma remains unclear, as very few patients with these cancer subtypes were included in the trials. In this case report, we present a patient with dMMR dedifferentiated endometrial carcinoma, treated with primary surgery to no gross residual disease, followed by carboplatin/paclitaxel chemotherapy and a short course of maintenance pembrolizumab. To date, the patient remains with a prolonged disease-free survival of 61 months, supporting the potential use of PD-1 inhibitors in the upfront treatment of unmeasurable, advanced-stage, dMMR dedifferentiated endometrial carcinoma.
Published: 2024
Full Text: View/download PDF

3. Environmental factors associated with invasive mold infections at a tertiary-care hospital

Author: Lindsey Tully, Schuyler L. Gaillard, Lucy Zheng, Tara Millson, Princy Kumar, and Joseph Timpone
Subjects: Infectious and parasitic diseases, RC109-216, Public aspects of medicine, RA1-1270
Abstract: Background: Invasive mold infections (IMIs) in hospitalized patients can result in significant morbidity and mortality. Environmental factors, such as hospital construction and negative air-pressure rooms (NAPRs), have been associated with hospital-acquired IMI. Increased utilization of NAPRs during the COVID-19 pandemic created a unique opportunity to examine the impact of NAPRs on IMI risk. Methods: From 2018 to present, a new pavilion was being constructed adjacent to our hospital. The Theradoc platform was used to identify positive mold cultures among adult patients hospitalized at our institution between March 1, 2017, and October 15, 2022. We performed a retrospective chart review of 262 mold isolates to determine patient demographics, timing of IMI, and their relationship to hospital construction and exposure to NAPR. IMI incidence was compared across 3 observation periods: (A) before hospital construction; (B) during hospital construction alone; and (C) during hospital construction and NAPR enhancement during the COVID-19 surge. Hospital-acquired IMI was defined as an infection that occurred >72 hours after admission. A REDCap database was created for data storage and R software was used for data analysis. Results: Of the 262 mold isolates identified, 61 cases were classified as IMI, of which 29 were hospital-acquired IMI. The mean age of IMI patients was 51.8 years, and 55.2% were male. Among them, 20.7% were exposed to NAPR during admission; 65.5.% were immunocompromised; and 2 patients were diagnosed with COVID-19. The all-cause mortality rate among hospital-acquired IMI cases was 79.3% (23 of 29). Also, 82.8% of hospital-acquired IMI cases were respiratory in nature, with 83.3% of these cases due to Aspergillus spp. Yearly rates of hospital-acquired IMI were 3.0 before construction versus 5.6 during construction (periods B and C). Yearly rates of hospital-acquired IMI, respiratory IMI, and invasive pulmonary aspergillosis by period were as follows: Period A had 3 hospital-acquired IMI cases per year, 2 hospital-acquired respiratory IMI cases per year, and 3 hospital-acquired invasive pulmonary aspergillosis cases per year. Period B had 4.5 hospital-acquired IMI cases per year, 3.5 hospital-acquired respiratory IMI cases per year, and 3.0 hospital-acquired invasive pulmonary aspergillosis cases per year. Period C had 6.5 hospital-acquired IMI cases per year, 5.4 hospital-acquired respiratory IMI cases per year, and 5.0 hospital-acquired invasive pulmonary aspergillosis cases per year. Conclusions: Hospital-acquired IMI was associated with a high mortality. Our data demonstrate a >2-fold increase in yearly incidence of hospital-acquired IMI before construction compared with during construction in association with increased implementation of NAPR. We have now reversed the trend in NAPR at our hospital’s designated COVID-19 units.
Published: 2023
Full Text: View/download PDF

4. Stress hormones are associated with inflammatory cytokines and attenuation of T-cell function in the ascites from patients with high grade serous ovarian cancer

Author: Alexandra N. Aquino-Acevedo, Hope Knochenhauer, Yesenia Castillo-Ocampo, Melanie Ortiz-León, Yadiel A. Rivera-López, Camily Morales-López, Melanie E. Cruz-Robles, Elvin R. Hernández-Cordero, Shonagh Russell, Regina Whitaker, Margarita Bonilla-Claudio, Dung-Tsa Chen, Julie Dutil, Stephanie L. Gaillard, John S. Yi, Rebecca A. Previs, and Guillermo N. Armaiz-Pena
Subjects: Ovarian cancer, Ascites, Cytokines, T-cells, Norepinephrine, Epinephrine, Neurosciences. Biological psychiatry. Neuropsychiatry, RC321-571
Abstract: Mounting evidence suggests that chronic stress and subsequent distress can promote ovarian cancer progression. These altered psychological states have been linked to sustained release of stress hormones, activation of the β-adrenergic receptors in ovarian cancer cells, and induction of pro-tumoral signaling pathways. In addition, data suggest that chronic stress promotes an inflammatory landscape highlighted by increased infiltration of tumor-associated macrophages into the ovarian tumor microenvironment (TME). In ovarian cancer, ascites is a unique TME comprised of tumor, and immune cells, which secrete pro-tumoral cytokines and chemokines that modulate tumor-associated immunity. However, our knowledge about how stress hormones impact the ascites TME remains limited. We hypothesized that the ascites harbors measurable levels of stress hormones, and accumulation of these in the ascites generates a pro-tumorigenic, inflammatory, and immunosuppressive TME. We evaluated ascites samples from 49 patients with high grade serous ovarian cancer (HGSOC) and quantified cortisol and stress hormones metabolites, metanephrine (MN), and normetanephrine (NMN) in all samples. We also measured 38 individual cytokines in the ascites, including several pro-inflammatory cytokines, such as IL-6, which were positively correlated to MN or NMN levels of those samples. Conversely, we found cortisol levels were negatively correlated to several pro-inflammatory cytokines. As T-cells are integral to the TME and our analyses identified cytokines in the ascites known to modulate T-cell function, we characterized ascites-derived T-cells and assessed the impact of stress hormones on the T-cell phenotype. Our data show an altered CD4+/CD8+ T-cell ratio and a heterogeneous expression of exhaustion markers in T-cells from the ascites, while ascites-derived CD8+ T-cells exposed to epinephrine had decreased co-expression CD38 and Granzyme B. To extend these findings to animal models, we subjected ovarian cancer-bearing mice to daily restraint stress, which resulted in increased tumor growth in two models. Congruent with our human analyses, we detected corticosterone, MN, and NMN in the ascites from tumor-bearing mice, and these stress hormones correlated with several inflammatory cytokines. Moreover, daily restraint stress leads to increased CD4+PD-1+/CD8+PD-1+ T-cell ratio in the ovarian tumor microenvironment. Overall, these data highlight a role of stress hormones in the ascites TME as a driver of tumor-associated inflammation, T-cell suppression, and disease progression.
Published: 2022
Full Text: View/download PDF

5. Translational randomized phase II trial of cabozantinib in combination with nivolumab in advanced, recurrent, or metastatic endometrial cancer

Author: John Wright, Stephanie Lheureux, Ilaria Colombo, Ramy Gadalla, Pamela S Ohashi, Xuan Li, Lisa Wang, Matthew S Block, David G Brooks, Andrea Jewell, Carolyn McCourt, Eugenia Girda, Sarah Temkin, Gini F Fleming, Linda Duska, Daniela E Matei, Panagiotis A Konstantinopoulos, Ben X Wang, Stephanie L Gaillard, Michael McHale, Floor J Backes, Theresa L Werner, Siobhan Kehoe, Rachel Wildman, Shirin Soleimani, Scott Lien, and Trevor Pugh
Subjects: Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282
Abstract: Background Combining immunotherapy and antiangiogenic agents is a promising treatment strategy in endometrial cancer. To date, no biomarkers for response have been identified and data on post-immunotherapy progression are lacking. We explored the combination of a checkpoint inhibitor (nivolumab) and an antiangiogenic agent (cabozantinib) in immunotherapy-naïve endometrial cancer and in patients whose disease progressed on previous immunotherapy with baseline biopsy for immune profiling.Patients and methods In this phase II trial (ClinicalTrials.gov NCT03367741, registered December 11, 2017), women with recurrent endometrial cancer were randomized 2:1 to nivolumab with cabozantinib (Arm A) or nivolumab alone (Arm B). The primary endpoint was Response Evaluation Criteria in Solid Tumors-defined progression-free survival (PFS). Patients with carcinosarcoma or prior immune checkpoint inhibitor received combination treatment (Arm C). Baseline biopsy and serial peripheral blood mononuclear cell (PBMC) samples were analyzed and associations between patient outcome and immune data from cytometry by time of flight (CyTOF) and PBMCs were explored.Results Median PFS was 5.3 (90% CI 3.5 to 9.2) months in Arm A (n=36) and 1.9 (90% CI 1.6 to 3.4) months in Arm B (n=18) (HR=0.59, 90% CI 0.35 to 0.98; log-rank p=0.09, meeting the prespecified statistical significance criteria). The most common treatment-related adverse events in Arm A were diarrhea (50%) and elevated liver enzymes (aspartate aminotransferase 47%, alanine aminotransferase 42%). In-depth baseline CyTOF analysis across treatment arms (n=40) identified 35 immune-cell subsets. Among immunotherapy-pretreated patients in Arm C, non-progressors had significantly higher proportions of activated tissue-resident (CD103+CD69+) ɣδ T cells than progressors (adjusted p=0.009).Conclusions Adding cabozantinib to nivolumab significantly improved outcomes in heavily pretreated endometrial cancer. A subgroup of immunotherapy-pretreated patients identified by baseline immune profile and potentially benefiting from combination with antiangiogenics requires further investigation.
Published: 2022
Full Text: View/download PDF

6. Development of small molecule inhibitors targeting PBX1 transcription signaling as a novel cancer therapeutic strategy

Author: Yao-An Shen, Jin Jung, Geoffrey D. Shimberg, Fang-Chi Hsu, Yohan Suryo Rahmanto, Stephanie L. Gaillard, Jiaxin Hong, Jürgen Bosch, Ie-Ming Shih, Chi-Mu Chuang, and Tian-Li Wang
Subjects: Chemistry, Cancer, Science
Abstract: Summary: PBX1 is a transcription factor involved in diverse cellular functions including organ development, stem cell renewal, and tumorigenesis. PBX1 is localized at chr1q23.3, a frequently amplified chromosomal region, and it is overexpressed in many human malignancies. Cancer cells with elevated PBX1 signaling are particularly vulnerable to PBX1 withdrawal. We designed a series of small molecule compounds capable of docking to the interface between PBX1 and its cognate DNA target sequence. Among them, T417 is found to be a lead compound. In cell-based assays, T417 significantly suppressed self-renewal and proliferation of cancer cells expressing high levels of PBX1. T417 also re-sensitized platinum-resistant ovarian tumors to carboplatin. T417 did not affect healthy tissues likely due to their lower PBX1 expression levels. Therefore, targeting PBX-DNA interface can be a promising strategy for treating human tumors reliant on PBX1 for survival.
Published: 2021
Full Text: View/download PDF

7. Uterine MEIS1::NCOA2 Fusion Sarcoma With Lung Metastasis: A Case Report and Review of the Literature

Author: Deyin Xing, Christian F. Meyer, John M. Gross, Pedram Argani, Chien-Fu Hung, T.-C. Wu, Russell Vang, Deborah K. Armstrong, and Stéphanie L. Gaillard
Subjects: Obstetrics and Gynecology, Pathology and Forensic Medicine
Published: 2023

8. Molecular classification of endometrial cancer: entering an era of precision medicine

Author: Alison, Goulder and Stéphanie L, Gaillard
Subjects: Oncology, Humans, Obstetrics and Gynecology, Female, General Medicine, Precision Medicine, Endometrial Neoplasms
Published: 2022

9. Regulation of Mus81-Eme1 structure-specific endonuclease by Eme1 SUMO-binding and Rad3ATR kinase is essential in the absence of Rqh1BLM helicase

Author: Cédric Giaccherini, Sarah Scaglione, Stéphane Coulon, Pierre-Marie Dehé, Pierre-Henri L. Gaillard, Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC), and Coulon, Stéphane
Subjects: DNA Replication, Cancer Research, [SDV.GEN]Life Sciences [q-bio]/Genetics, Point mutation, DNA recombination, DNA Helicases, [SDV.GEN] Life Sciences [q-bio]/Genetics, Saccharomyces cerevisiae, Endonucleases, Cell cycle and cell division, DNA-Binding Proteins, Schizosaccharomyces pombe, Schizosaccharomyces, Genetics, DNA damage, Helicases, Schizosaccharomyces pombe Proteins, Phosphorylation, Molecular Biology, Genetics (clinical), Ecology, Evolution, Behavior and Systematics
Abstract: The Mus81-Eme1 structure-specific endonuclease is crucial for the processing of DNA recombination and late replication intermediates. In fission yeast, stimulation of Mus81-Eme1 in response to DNA damage at the G2/M transition relies on Cdc2CDK1 and DNA damage checkpoint-dependent phosphorylation of Eme1 and is critical for chromosome stability in absence of the Rqh1BLM helicase. Here we identify Rad3ATR checkpoint kinase consensus phosphorylation sites and two SUMO interacting motifs (SIM) within a short N-terminal domain of Eme1 that is required for cell survival in absence of Rqh1BLM. We show that direct phosphorylation of Eme1 by Rad3ATR is essential for catalytic stimulation of Mus81-Eme1. Chk1-mediated phosphorylation also contributes to the stimulation of Mus81-Eme1 when combined with phosphorylation of Eme1 by Rad3ATR. Both Rad3ATR- and Chk1-mediated phosphorylation of Eme1 as well as the SIMs are critical for cell fitness in absence of Rqh1BLM and abrogating bimodal phosphorylation of Eme1 along with mutating the SIMs is incompatible with rqh1Δ cell viability. Our findings unravel an elaborate regulatory network that relies on the poorly structured N-terminal domain of Eme1 and which is essential for the vital functions Mus81-Eme1 fulfills in absence of Rqh1BLM.
Published: 2021

10. Présentation clinique et performance de la bandelette urinaire pour le diagnostic d’infection urinaire en population gériatrique

Author: M. Laycuras, L. Leichowski, E. Fercot, Laurent Teillet, Sophie Moulias, Z. Mekerta, A. de Thezy, A. Dinh, M. Gourdon, A.-L. Coudert, Florence Muller, C.-L. Peng, Marion Pépin, Frédérique Bouchand, B. Davido, M. Le Crane, M. Rottman, B. Denis, R. Mahamdia, J. L. Gaillard, M. Coudert, Clara Duran, Z. Seridi, and V. Sivadon-Tardy
Subjects: Gynecology, 0303 health sciences, 03 medical and health sciences, medicine.medical_specialty, 0302 clinical medicine, Infection urinaire, 030306 microbiology, business.industry, Gastroenterology, Internal Medicine, Medicine, 030212 general & internal medicine, business
Abstract: Resume Introduction L’infection urinaire (IU) constitue la deuxieme cause d’infection bacterienne communautaire chez le sujet âge. Ce diagnostic est difficile car les symptomes sont souvent atypiques. Nous presentons les caracteristiques des patients ayant une IU parmi une population geriatrique (signes fonctionnels urinaires et/ou syndrome infectieux). Nous avons egalement evalue la sensibilite et la specificite de la bandelette urinaire (BU) pour le diagnostic d’IU dans cette population. Patients et methode Nous avons realise une etude observationnelle, prospective, monocentrique, d’avril 2017 a janvier 2018, incluant tous les patients hospitalises en geriatrie, presentant des signes fonctionnels urinaires et/ou un syndrome infectieux sans point d’appel, pour lesquels un examen cytobacteriologique des urines (ECBU) a ete demande. Une BU etait systematiquement realisee pour tous ces patients. Les caracteristiques cliniques et biologiques des patients ont ete etudiees selon le diagnostic definitif. En outre, les resultats des BU ont ete compares a ceux des ECBU en cas de diagnostic final d’IU. Resultats Au total, 165 patients ont ete inclus. Parmi eux, 67 (40,6 %) avaient une IU et 98 (59,4 %) avaient un autre diagnostic. Il y avait significativement plus de femmes ( p p La BU presentait une sensibilite elevee (92 %) et une VPN elevee (91 %) interessantes dans notre population. Conclusion En cas d’IU chez le sujet âge, peu de criteres sont discriminants avec une autre origine. Par ailleurs, une BU negative suggere l’absence d’IU en raison de sa bonne valeur predictive negative.
Published: 2019

11. Association between frontal cortico-limbic white-matter microstructure and risk for pediatric depression

Author: John D. E. Gabrieli, Allison Green, Joseph Biederman, James Capella, Mai Uchida, Caroline Kelberman, Schuylar L. Gaillard, and Yuwen Hung
Subjects: Male, medicine.medical_specialty, Neuroscience (miscellaneous), Audiology, behavioral disciplines and activities, Article, White matter, Fractional anisotropy, mental disorders, Medicine, Cingulum (brain), Humans, Radiology, Nuclear Medicine and imaging, Bipolar disorder, Child Behavior Checklist, Child, Depressive Disorder, Major, business.industry, Depression, medicine.disease, Emotional dysregulation, White Matter, Psychiatry and Mental health, medicine.anatomical_structure, Diffusion Tensor Imaging, Major depressive disorder, Anisotropy, Female, business, Diffusion MRI
Abstract: This study aimed to identify white-matter microstructural characteristics associated with risk for pediatric major depressive disorder (MDD) measured by the Child Behavior Checklist (CBCL) Anxiety/Depression scores. Children (N = 32) of both sexes, aged 6–12, underwent T1-weighted whole-head anatomical and diffusion-weighted imaging. Each participant's mean diffusion measure image was generated and thinned to create an alignment-invariant tract representation. Voxel-wise analysis on the resulting map was carried out in Track Based Spatial Statistics (TBSS) using general linear models by regressing the CBCL-Anxiety/Depression score against measures of diffusion tensor imaging (DTI). We also compared these results with prior DTI findings from the same children associated with CBCL-Emotion Dysregulation profile, an indicator for bipolar disorder. TBSS voxel-wise analysis showed a significant negative correlation between fractional anisotropy (FA) and CBCL-Anxiety/Depression scores localized in the right anterior cingulum and connected corpus callosal region. The negative FA correlations in these regions were greater in CBCL-Anxiety/ Depression scores compared to CBCL-Emotional Dysregulation scores. Reduced white-matter connectivity in the anterior cingulum and connected corpus callosal region may represent a biomarker of risk for pediatric MDD. These results may help identify brain differences associated with the development of MDD, and assist with earlier clinical identification of pediatric MDD.
Published: 2021

12. Translational randomized phase II trial of cabozantinib in combination with nivolumab in advanced, recurrent, or metastatic endometrial cancer

Author: Stephanie Lheureux, Daniela E Matei, Panagiotis A Konstantinopoulos, Ben X Wang, Ramy Gadalla, Matthew S Block, Andrea Jewell, Stephanie L Gaillard, Michael McHale, Carolyn McCourt, Sarah Temkin, Eugenia Girda, Floor J Backes, Theresa L Werner, Linda Duska, Siobhan Kehoe, Ilaria Colombo, Lisa Wang, Xuan Li, Rachel Wildman, Shirin Soleimani, Scott Lien, John Wright, Trevor Pugh, Pamela S Ohashi, David G Brooks, and Gini F Fleming
Subjects: Pharmacology, Cancer Research, Nivolumab, Oncology, Pyridines, Immunology, Leukocytes, Mononuclear, Humans, Molecular Medicine, Immunology and Allergy, Anilides, Female, Endometrial Neoplasms
Abstract: BackgroundCombining immunotherapy and antiangiogenic agents is a promising treatment strategy in endometrial cancer. To date, no biomarkers for response have been identified and data on post-immunotherapy progression are lacking. We explored the combination of a checkpoint inhibitor (nivolumab) and an antiangiogenic agent (cabozantinib) in immunotherapy-naïve endometrial cancer and in patients whose disease progressed on previous immunotherapy with baseline biopsy for immune profiling.Patients and methodsIn this phase II trial (ClinicalTrials.gov NCT03367741, registered December 11, 2017), women with recurrent endometrial cancer were randomized 2:1 to nivolumab with cabozantinib (Arm A) or nivolumab alone (Arm B). The primary endpoint was Response Evaluation Criteria in Solid Tumors-defined progression-free survival (PFS). Patients with carcinosarcoma or prior immune checkpoint inhibitor received combination treatment (Arm C). Baseline biopsy and serial peripheral blood mononuclear cell (PBMC) samples were analyzed and associations between patient outcome and immune data from cytometry by time of flight (CyTOF) and PBMCs were explored.ResultsMedian PFS was 5.3 (90% CI 3.5 to 9.2) months in Arm A (n=36) and 1.9 (90% CI 1.6 to 3.4) months in Arm B (n=18) (HR=0.59, 90% CI 0.35 to 0.98; log-rank p=0.09, meeting the prespecified statistical significance criteria). The most common treatment-related adverse events in Arm A were diarrhea (50%) and elevated liver enzymes (aspartate aminotransferase 47%, alanine aminotransferase 42%). In-depth baseline CyTOF analysis across treatment arms (n=40) identified 35 immune-cell subsets. Among immunotherapy-pretreated patients in Arm C, non-progressors had significantly higher proportions of activated tissue-resident (CD103+CD69+) ɣδ T cells than progressors (adjusted p=0.009).ConclusionsAdding cabozantinib to nivolumab significantly improved outcomes in heavily pretreated endometrial cancer. A subgroup of immunotherapy-pretreated patients identified by baseline immune profile and potentially benefiting from combination with antiangiogenics requires further investigation.
Published: 2022

13. Non-operative treatment of children with simple appendicitis: long-term follow-up (5 years) in a prospective cohort study

Author: Huibert A. Cense, J. H. van der Lee, K H In 't Hof, L.W.E. van Heurn, Max Knaapen, Ramon R. Gorter, C. M. F. Kneepkens, Hugo A. Heij, Marc H. W. A. Wijnen, E L Gaillard, Roel Bakx, Surgery, Pediatrics, General practice, Pediatric surgery, Amsterdam Reproduction & Development (AR&D), Other Research, AGEM - Re-generation and cancer of the digestive system, Graduate School, Paediatric Surgery, Amsterdam Gastroenterology Endocrinology Metabolism, General Paediatrics, APH - Methodology, and APH - Quality of Care
Subjects: medicine.medical_specialty, business.industry, Long term follow up, General surgery, MEDLINE, Non operative treatment, Unnecessary Procedures, medicine.disease, Appendicitis, Anti-Bacterial Agents, Medicine, Appendectomy, Humans, Surgery, business, Prospective cohort study, Child, Simple (philosophy), Follow-Up Studies, Randomized Controlled Trials as Topic
Abstract: Long-term results after non-operative treatment for children with simple appendicitis seem promising, possibly avoiding appendicectomy in 70 per cent of children after a median follow-up of 5 years. The need for delayed appendicectomy more than 2 years after the initial treatment is rare (0–5 per cent) and no complications occurred past 1 year, including children who underwent delayed appendicectomy.
Published: 2020

14. Cingulum-Callosal white-matter microstructure associated with emotional dysregulation in children: A diffusion tensor imaging study

Author: Caroline Kelberman, Susan Whitfield-Gabrieli, John D. E. Gabrieli, Dina R. Hirshfeld-Becker, Hilary Woodworth, Anastasia Yendiki, Xiaoqian J. Chai, Yuwen Hung, James Capella, Satrajit S. Ghosh, Schuyler L. Gaillard, Kelly Kadlec, Mai Uchida, Joseph Biederman, and Mathias Goncalves
Subjects: Male, Adolescent, Cognitive Neuroscience, Corpus callosum, Emotions, Emotional dysregulation, lcsh:Computer applications to medicine. Medical informatics, behavioral disciplines and activities, 050105 experimental psychology, lcsh:RC346-429, 03 medical and health sciences, 0302 clinical medicine, Limbic system, Cingulum, Fractional anisotropy, medicine, Humans, 0501 psychology and cognitive sciences, Radiology, Nuclear Medicine and imaging, Child Behavior Checklist, Child, Depression (differential diagnoses), lcsh:Neurology. Diseases of the nervous system, business.industry, 05 social sciences, Brain, Regular Article, medicine.disease, Mental health, White Matter, Diffusion Magnetic Resonance Imaging, Diffusion Tensor Imaging, Neurology, Mood disorders, Anxiety, Anisotropy, lcsh:R858-859.7, Female, Neurology (clinical), medicine.symptom, Nerve Net, business, 030217 neurology & neurosurgery, Clinical psychology
Abstract: Highlights • Emotional dysregulation is related to differences in cingulum-callosal microstructural integrity. • Emotional dysregulation is associated with increases in radial diffusivity and decreases in fractional anisotropy in cingulum-callosal bundles. • Cingulum-callosal bundles may be susceptibility neuromarkers for pediatric mood disorders., Emotional dysregulation symptoms in youth frequently predispose individuals to increased risk for mood disorders and other mental health difficulties. These symptoms are also known as a behavioral risk marker in predicting pediatric mood disorders. The underlying neural mechanism of emotional dysregulation, however, remains unclear. This study used the diffusion tensor imaging (DTI) technique to identify anatomically specific variation in white-matter microstructure that is associated with pediatric emotional dysregulation severity. Thirty-two children (mean age 9.53 years) with varying levels of emotional dysregulation symptoms were recruited by the Massachusetts General Hospital and underwent the DTI scans at Massachusetts Institute of Technology. Emotional dysregulation severity was measured by the empirically-derived Child Behavior Checklist Emotional Dysregulation Profile that includes the Attention, Aggression, and Anxiety/Depression subscales. Whole-brain voxel-wise regression tests revealed significantly increased radial diffusivity (RD) and decreased fractional anisotropy (FA) in the cingulum-callosal regions linked to greater emotional dysregulation in the children. The results suggest that microstructural differences in cingulum-callosal white-matter pathways may manifest as a neurodevelopmental vulnerability for pediatric mood disorders as implicated in the clinical phenotype of pediatric emotional dysregulation. These findings may offer clinically and biologically relevant neural targets for early identification and prevention efforts for pediatric mood disorders.
Published: 2020

15. Range-Wide and Regional Patterns of Population Structure and Genetic Diversity in the Gopher Tortoise

Author: Henry R. Mushinsky, Tracey D. Tuberville, S.C. Sweat, T.W. Hentges, Earl D. McCoy, Joshua R. Ennen, Carl P. Qualls, Daniel L. Gaillard, Brian R. Kreiser, Roger D. Birkhead, and Matthew J. Aresco
Subjects: 0106 biological sciences, 0301 basic medicine, Conservation genetics, Genetic diversity, education.field_of_study, Ecology, Tortoise, Range (biology), Population, Biology, biology.organism_classification, 010603 evolutionary biology, 01 natural sciences, 03 medical and health sciences, Phylogeography, 030104 developmental biology, Polyphemus, Threatened species, Animal Science and Zoology, education, Ecology, Evolution, Behavior and Systematics, Nature and Landscape Conservation
Abstract: The gopher tortoise (Gopherus polyphemus) has experienced dramatic population declines throughout its distribution in the southeastern United States and is federally listed as threatened in the area west of the Tombigbee and Mobile rivers. While there is molecular support for recognizing the listed portion of the range as genetically distinct, other research has suggested that additional population structure exists at both range-wide and regional scales. In this study, we sought to comprehensively define genetic population structure at both spatial scales by doubling the data available in terms of the number of sampling sites, individuals, and microsatellite loci compared to previously published work. We also compared patterns of genetic diversity, gene flow, and demographic history across the range. We collected 933 individuals from 47 sampling sites across the range and genotyped them for 20 microsatellite loci. Our range-wide analyses supported the recognition of five genetic groups (or regions) delineated by the Tombigbee and Mobile rivers, Apalachicola and Chattahoochee rivers, and the transitional areas between several physiographic province sections of the Coastal Plains (i.e., Eastern Gulf, Sea Island, and Floridian). We found genetic admixture at sampling sites along the boundaries of these genetically defined groups. We detected some degree of additional genetic structure within each of the five regions. Notably, within the range listed as threatened under the Endangered Species Act, we found some support for two additional genetic groups loosely delineated by the Pascagoula and Chickasawhay rivers, and we detected four more genetic groups within the Florida region that seemed to reflect the influence of the local physiography. Additionally, our range-wide analysis found the periphery of the range had lower levels of genetic diversity relative to the core. We suggest that the five main genetic groups delineated in our study warrant recognition as management units in terms of conservation planning. Intraregional population structure also points to the potential importance of other barriers to gene flow at finer spatial scales, although additional work is needed to better delineate these genetic groups.
Published: 2017

16. WRNIP1 Protects Reversed DNA Replication Forks from SLX4-Dependent Nucleolytic Cleavage

Author: Sandra Kummer, Pierre-Henri L. Gaillard, Sebastian Wild, Kerstin Gari, Bartlomiej Porebski, Sarah Scaglione, Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC), Aix Marseille Université (AMU), Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS), University of Zurich, and Porebski, Bartlomiej
Subjects: 0301 basic medicine, Genome instability, ATPase, 610 Medicine & health, [SDV.CAN]Life Sciences [q-bio]/Cancer, 02 engineering and technology, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Cleavage (embryo), Article, 03 medical and health sciences, chemistry.chemical_compound, WRNIP1, lcsh:Science, Molecular Biology, ComputingMilieux_MISCELLANEOUS, 1000 Multidisciplinary, Multidisciplinary, biology, Chemistry, 10061 Institute of Molecular Cancer Research, DNA replication, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], Cell Biology, Biological Sciences, 021001 nanoscience & nanotechnology, 3. Good health, Cell biology, 030104 developmental biology, [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics, Junction point, Molecular mechanism, biology.protein, 570 Life sciences, lcsh:Q, 0210 nano-technology, DNA
Abstract: Summary During DNA replication stress, stalled replication forks need to be stabilized to prevent fork collapse and genome instability. The AAA + ATPase WRNIP1 (Werner Helicase Interacting Protein 1) has been implicated in the protection of stalled replication forks from nucleolytic degradation, but the underlying molecular mechanism has remained unclear. Here we show that WRNIP1 exerts its protective function downstream of fork reversal. Unexpectedly though, WRNIP1 is not part of the well-studied BRCA2-dependent branch of fork protection but seems to protect the junction point of reversed replication forks from SLX4-mediated endonucleolytic degradation, possibly by directly binding to reversed replication forks. This function is specific to the shorter, less abundant, and less conserved variant of WRNIP1. Overall, our data suggest that in the absence of BRCA2 and WRNIP1 different DNA substrates are generated at reversed forks but that nascent strand degradation in both cases depends on the activity of exonucleases and structure-specific endonucleases., Graphical Abstract, Highlights • WRNIP1, as BRCA2, protects stalled replication forks downstream of fork reversal • WRNIP1 and BRCA2 act in two different branches of the fork protection pathway • WRNIP1 protects from SLX4-mediated nucleolytic cleavage, possibly by direct binding • Fork protection function is specific to the shorter isoform of WRNIP1, Biological Sciences; Molecular Biology; Cell Biology
Published: 2019

17. [Clinical presentation and performance of urine dipstick for diagnosis of urinary infection in geriatric population]

Author: M, Coudert, M, Pépin, A, de Thezy, E, Fercot, M, Laycuras, A-L, Coudert, C, Duran, F, Bouchand, B, Davido, M, Le Crane, B, Denis, F, Muller, M, Gourdon, C-L, Peng, R, Mahamdia, Z, Mekerta, Z, Seridi, J-L, Gaillard, L, Leichowski, S, Moulias, M, Rottman, V, Sivadon-Tardy, L, Teillet, and A, Dinh
Subjects: Aged, 80 and over, Male, Bacteriuria, Geriatrics, Predictive Value of Tests, Urinary Tract Infections, Humans, Female, Prospective Studies, Urinalysis, Sensitivity and Specificity
Abstract: Urinary tract infections (UTI) are the second cause of community-acquired bacterial infections in the elderly. Distinguishing symptomatic UTI from asymptomatic bacteriuria is problematic, as older adults are less likely to present with localized urinary symptoms. We evaluated characteristics of patients presenting UTI among elderly with sepsis. Moreover, we aimed to evaluate the sensibility and specificity of urine dipstick tests in the diagnosis of UTI in geriatric population.We led a prospective, monocentric, observational study between April 2017 and January 2018. We included patients hospitalized in geriatric wards, who were prescribed urine culture for UTI symptoms or/and infection without primary sites for which a urine culture was prescribed. Dipstick urinalyses were performed for all patients. Clinical and biological characteristics of all patients were compared according to the final diagnosis of UTI. Moreover, results of dipstick tests were evaluated for the diagnosis of UTI in this population.Among 165 patients, 67 (40.6 %) had a UTI and 98 (59.4 %) had another diagnosis. These two groups were comparable for age and daily-living activities. In the UTI group, the proportion of women was higher than in the other group (P0.05), and mean MMSE score was lower (P0.05). Positive urine dipstick test for leukocytes and/or nitrites had high sensitivity (92 %), but low specificity (50 %). Negative predictive value of this test was high (91 %).For suspicion of UTI among elderly, few criteria are specific. Negative dipstick tests can suggest an absence of UTI due to its high negative predictive value.
Published: 2019

18. Concordance et dissociation des seuils de fatigue électromyographiques et des seuils ventilatoires chez le patient en réadaptation cardiaque

Author: L. Gaillard, S. Delanaud, T. Porcher, Thierry Weissland, A. Barnabé, M. Ghannem, N. Jaunet, P.-M. Leprêtre, Adaptations Physiologiques à l'Exercice et Réadaptation à l'effort - UR UPJV 3300 (APERE), Université de Picardie Jules Verne (UPJV)-CHU Amiens-Picardie, Biomécanique et génie biomédical (BIM), Centre National de la Recherche Scientifique (CNRS), Centre interuniversitaire de recherche et d'ingenierie des matériaux (CIRIMAT), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)
Subjects: Gynecology, 03 medical and health sciences, medicine.medical_specialty, 0302 clinical medicine, business.industry, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], Medicine, 030229 sport sciences, 030204 cardiovascular system & hematology, Cardiology and Cardiovascular Medicine, business, ComputingMilieux_MISCELLANEOUS
Abstract: Resume La prescription d’exercice repose generalement sur la determination des seuils ventilatoires (SV1, SV2) lors d’une epreuve d’effort (CPX). Chez le sujet sain, l’atteinte des seuils ventilatoires est contemporaine de modifications de la valeur RMS de l’activite electromyographique de surface du vastus lateralis (EMGth1, EMGth2). Objectif de l’etude Observer si EMGth1 et EMGth2 apparaissent en concordance avec SV1 et SV2 chez le patient cardiaque. Patients et methode Trente-quatre sujets (62,1 ± 7,3 ans, 172,1 ± 6,3 cm, 81,3 ± 15,3 kg, IMC : 27,3 ± 4,1) ont realise un CPX sur bicyclette a 60 rpm. SV1 correspondait a la cassure dans la relation dioxyde de carbone produit (CO2) – consommation d’oxygene (VO2) ; SV2 a l’intensite pour laquelle l’equivalent respiratoire en CO2 augmentait. EMGth1 et EMGth2 etaient definis comme les premier et second points de rupture dans la relation RMS-intensite. Resultats Les valeurs pics de VO2 (16,3 ± 4,6 mL·min−1·kg−1) et de frequence cardiaque (106,7 ± 13,8 bpm) ont ete atteintes a 112,9 ± 38,5 w (PMT) ; SV1 et SV2 a 71,1 ± 25,9 w (62,5 ± 5,5 % de PMT) et 87,9 ± 28,6 w (78,0 ± 5,1 % de PMT). Chez ces sujets, la relation RMS-intensite presentait deux points de cassure, identifies comme EMGth1 a 68,0 ± 24,7w et EMGth2 a 88,5 ± 30,1w, soit a 60,0 ± 7,6 et 78,6 ± 5,0 % de PMT. Une difference significative a ete observee entre les intensites a EMGth1 et SV1 (p = 0,004), mais pas entre EMGth2 et SV2 (p = 0,13) avec un faible effet de la taille de l’echantillon. Conclusion L’apparition de EMGth1 avant SV1 suppose un lien de causalite entre conditionnement musculaire et reponses ventilatoires a l’effort ; lien a prendre en compte dans la prescription d’activites physiques adaptees du patient cardiaque.
Published: 2016

19. A snapshot of the microbiome of Amblyomma tuberculatum ticks infesting the gopher tortoise, an endangered species

Author: Shahid Karim, Daniel L. Gaillard, Jaclyn Williams, Nabanita Mukherjee, and Khemraj Budachetri
Subjects: 0301 basic medicine, Ixodidae, Tortoise, 030231 tropical medicine, Tick, Microbiology, Article, 03 medical and health sciences, 0302 clinical medicine, Animals, biology, Ecology, Endangered Species, Amblyomma tuberculatum, bacterial infections and mycoses, biology.organism_classification, Gastrointestinal Microbiome, Tick Infestations, Turtles, Spotted fever, Gastrointestinal Tract, 030104 developmental biology, Infectious Diseases, Rickettsia, Insect Science, bacteria, Pyrosequencing, Female, Parasitology, Proteobacteria
Abstract: The gopher tortoise tick, Amblyomma tuberculatum, has a unique relationship with the gopher tortoise, Gopherus polyphemus, found in sandy habitats across the southeastern United States. We aimed to understand the overall bacterial community associated with A. tuberculatum while also focusing on spotted fever group Rickettsia. These tortoises in the Southern Mississippi region are a federally threatened species; therefore, we have carefully trapped the tortoises and removed the species-specific ticks attached to them. Genomic DNA was extracted from individual ticks and used to explore overall bacterial load using pyrosequencing of bacterial 16S rRNA on 454-sequencing platform. The spotted fever group of Rickettsia was explored by amplifying rickettsial outer membrane protein A (rompA) gene by nested PCR. Sequencing results revealed 330 bacterial operational taxonomic units (OTUs) after all the necessary curation of sequences. Four whole A. tuberculatum ticks showed Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes as the most dominant phyla with a total of 74 different bacterial genera detected. Together Rickettsiae and Francisella showed >85% abundance, thus dominating the bacterial community structure. Partial sequences obtained from ompA amplicons revealed the presence of an uncharacterized Rickettsia similar to the Rickettsial endosymbiont of A. tuberculatum. This is the first preliminary profile of a complete bacterial community from gopher tortoise ticks and warrants further investigation regarding the functional role of Rickettsial and Francisella-like endosymbionts in tick physiology.
Published: 2016

20. High Connectivity Observed in Populations of Ringed Sawbacks,Graptemys oculifera, in the Pearl and Bogue Chitto Rivers Using Six Microsatellite Loci

Author: Will Selman, Keri Landry, Daniel L. Gaillard, Robert L. Jones, Carl P. Qualls, and Brian R. Kreiser
Subjects: education.field_of_study, biology, Ecology, Range (biology), Population, Population genetics, Aquatic Science, engineering.material, biology.organism_classification, River drainage, Drainage system (geomorphology), engineering, Microsatellite, Animal Science and Zoology, Graptemys oculifera, education, Pearl, Ecology, Evolution, Behavior and Systematics
Abstract: Graptemys oculifera is endemic to the Pearl River drainage system in Louisiana and Mississippi, and due to this limited range it is vulnerable to environmental changes. Many impacts to this drainage system are due to anthropogenic activities, and alterations may negatively impact the connectivity among populations of G. oculifera. Previous studies show populations below the Ross Barnett Reservoir might be undergoing population declines, suggesting limited movement among populations. In addition to anthropogenic effects, the drainage geomorphology might also play a role in shaping population connectivity, as the sister species of G. oculifera, G. flavimaculata, contains two distinct populations (mainstem Pascagoula and Escatawpa River) and possible subpopulations within the Pascagoula (upper Leaf River, upper Chickasawhay River, and lower sections of the Pascagoula River). We used six polymorphic microsatellite loci to analyze the population genetics of G. oculifera at eight sites in the Pearl River draina...
Published: 2015

21. Author Correction: SLX4 interacts with RTEL1 to prevent transcription-mediated DNA replication perturbations

Author: A. Takedachi, E. Despras, S. Scaglione, R. Guérois, J. H. Guervilly, M. Blin, S. Audebert, L. Camoin, Z. Hasanova, M. Schertzer, A. Guille, D. Churikov, I. Callebaut, V. Naim, M. Chaffanet, J. P. Borg, F. Bertucci, P. Revy, D. Birnbaum, A. Londoño-Vallejo, P. L. Kannouche, and P. H. L. Gaillard
Subjects: Structural Biology, Molecular Biology
Published: 2020

22. Fasciite nécrosante humaine à Nannizziopsis spp

Author: C. Charron, I. Bourgault-Villada, A. Dinh, C. Rodaix, B. Villain, A.-L. Roux, E. Sitterlé, S. Trad, Hélène Mascitti, E. Salomon, F. Coudert, Y. Hababou, V. Tardy, Anna Philibertine Soavelomandroso, Fanny Lanternier, M.-T. Duong, M.-E. Bougnoux-Andremont, M. Tourte, J.-L. Gaillard, and B. Pages
Subjects: Dermatology
Abstract: Introduction La fasciite necrosante est une infection rare des tissus sous-cutanes atteignant le fascia. Sa mortalite s’eleve a 30 % et sa prise en charge est urgente. L’immunodepression est un facteur de risque reconnu. De causes bacteriennes, elles peuvent etre mono ou polymicrobiennes, a germes aero- ou anaerobies. Les streptocoques du groupe A, enterobacteries, P. aeruginosa et les anaerobies sont le plus souvent impliques. L’origine mycologique est tres rare et exclusivement reservee aux immunodeprimes. Les champignons responsables decrits sont : Mucorales, Aspergillus et Candida. Aucun cas de fasciite necrosante humaine a Nannizziopsis spp. n’etait encore decrit. Observations Un homme africain de 56 ans vivant en France depuis longtemps est greffe renal depuis 2013. Il prend quotidiennement du mycophenolate mofetil, tacrolimus, prednisolone et oracilline. Il n’a pas voyage recemment et n’est pas seropositif VIH. Il est hospitalise en reanimation pour un choc septique sans porte d’entree trouvee avec un traitement probabiliste initial par imipeneme. Quatre jours apres l’admission, il presente une douleur intense de la jambe droite avec apparition de lesions cutanees evocatrices de fasciite necrosante motivant une prise en charge chirurgicale urgente ( Fig. 1 ). Les hemocultures initiales sont positives a S. marcescens, K. pneumonia et P. aeruginosa mais les prelevements peroperatoires ne les retrouveront pas. Ils objectiveront un champignon filamenteux contre lequel on adjoindra a la tazocilline-daptomycine initiee en postoperatoire immediat un traitement par amphotericine B. L’identification de Nannizziopsis spp. et son antifongigramme en centre de reference ont permis le relais par voriconazole. L’ETO n’objective pas d’endocardite, l’IRMc, la PL, l’examen ORL sont normales. La TDM TAP objective des images suspectes hepatiques peut-etre en lien avec une infection invasive a Nannizziopsis spp. L’examen cutane objective des nodules sous-cutanes diffus dont l’anatomopathologie avec coloration de Gomori-Grocott est en attente ( Fig. 2 ). Discussion Nannizziopsis spp. est un champignon keratinophilique connu des veterinaires pour causer des dermatophytoses et granulomes chez les reptiles. Sept cas d’infections invasives humaines a Nannizziopsis ont ete decrites chez les immunodeprimes mais aucune sous forme de fasciite necrosante. Les patients presentaient soit une atteinte pulmonaire, soit des nodules cerebraux, soit des osteites. Ici, comme dans les autres cas decrits, le patient est d’origine africaine mais il n’avait cependant pas voyage recemment. Il n’est pas en contact avec des reptiles. L’imipeneme donne initialement a pu favoriser le developpement fongique intense sur ce terrain. Conclusion Nous rapportons le premier cas de fasciite necrosante a Nannizziopsis spp. chez un patient greffe renal. Nannizziopsis spp. semble etre un champignon emergent en pathologie humaine de l’immunodeprime.
Published: 2019

23. Manubrio-sternal joint mobility during forced ventilation using non-invasive opto-electronic plethysmography: cases studies

Author: Nicolas Houel, L. Gaillard, L. Debraux, École supérieure d'ostéopathie (ESO), Laboratoire de Mathématiques de Reims (LMR), Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), and Université de Reims Champagne-Ardenne (URCA)
Subjects: Sternum, Biomedical Engineering, Bioengineering, Sternal angle, Xiphoid process, 03 medical and health sciences, 0302 clinical medicine, Medicine, 0601 history and archaeology, Joint (geology), ComputingMilieux_MISCELLANEOUS, 060101 anthropology, business.industry, Non invasive, [SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph], 06 humanities and the arts, General Medicine, Opto electronic plethysmography, Anatomy, Flat bone, Computer Science Applications, Human-Computer Interaction, medicine.anatomical_structure, 030228 respiratory system, Breathing, business
Abstract: Sternum is a flat bone composed of three parts: manubrium superiorly, xiphoid process inferiorly and the body in between (Selthofer et al. 2006). Manubrio-Sternal Joint (MSJ), also known as Louis’ ...
Published: 2019

24. STAR Collaboration

Author: B.I. Abelev, J. Adams, M.M. Aggarwal, Z. Ahammed, J. Amonett, B.D. Anderson, M. Anderson, D. Arkhipkin, G.S. Averichev, Y. Bai, J. Balewski, O. Barannikova, L.S. Barnby, J. Baudot, S. Bekele, V.V. Belaga, A. Bellingeri-Laurikainen, R. Bellwied, F. Benedosso, S. Bhardwaj, A. Bhasin, A.K. Bhati, H. Bichsel, J. Bielcik, J. Bielcikova, L.C. Bland, S.-L. Blyth, B.E. Bonner, M. Botje, J. Bouchet, A.V. Brandin, A. Bravar, M. Bystersky, R.V. Cadman, X.Z. Cai, H. Caines, M. Calderón de la Barca Sánchez, J. Castillo, O. Catu, D. Cebra, Z. Chajecki, P. Chaloupka, S. Chattopadhyay, H.F. Chen, J.H. Chen, J. Cheng, M. Cherney, A. Chikanian, W. Christie, J.P. Coffin, T.M. Cormier, M.R. Cosentino, J.G. Cramer, H.J. Crawford, D. Das, S. Das, M. Daugherity, M.M. de Moura, T.G. Dedovich, M. DePhillips, A.A. Derevschikov, L. Didenko, T. Dietel, P. Djawotho, S.M. Dogra, W.J. Dong, X. Dong, J.E. Draper, F. Du, V.B. Dunin, J.C. Dunlop, M.R. Dutta Mazumdar, V. Eckardt, W.R. Edwards, L.G. Efimov, V. Emelianov, J. Engelage, G. Eppley, B. Erazmus, M. Estienne, P. Fachini, R. Fatemi, J. Fedorisin, K. Filimonov, P. Filip, E. Finch, V. Fine, Y. Fisyak, J. Fu, C.A. Gagliardi, L. Gaillard, M.S. Ganti, V. Ghazikhanian, P. Ghosh, J.E. Gonzalez, Y.G. Gorbunov, H. Gos, O. Grebenyuk, D. Grosnick, S.M. Guertin, K.S.F.F. Guimaraes, N. Gupta, T.D. Gutierrez, B. Haag, T.J. Hallman, A. Hamed, J.W. Harris, W. He, M. Heinz, T.W. Henry, S. Hepplemann, B. Hippolyte, A. Hirsch, E. Hjort, A.M. Hoffman, G.W. Hoffmann, M.J. Horner, H.Z. Huang, S.L. Huang, E.W. Hughes, T.J. Humanic, G. Igo, P. Jacobs, W.W. Jacobs, P. Jakl, F. Jia, H. Jiang, P.G. Jones, E.G. Judd, S. Kabana, K. Kang, J. Kapitan, M. Kaplan, D. Keane, A. Kechechyan, V.Yu. Khodyrev, B.C. Kim, J. Kiryluk, A. Kisiel, E.M. Kislov, S.R. Klein, A. Kocoloski, D.D. Koetke, T. Kollegger, M. Kopytine, L. Kotchenda, V. Kouchpil, K.L. Kowalik, M. Kramer, P. Kravtsov, V.I. Kravtsov, K. Krueger, C. Kuhn, A.I. Kulikov, A. Kumar, A.A. Kuznetsov, M.A.C. Lamont, J.M. Landgraf, S. Lange, S. LaPointe, F. Laue, J. Lauret, A. Lebedev, R. Lednicky, C.-H. Lee, S. Lehocka, M.J. LeVine, C. Li, Q. Li, Y. Li, G. Lin, X. Lin, S.J. Lindenbaum, M.A. Lisa, F. Liu, H. Liu, J. Liu, L. Liu, Z. Liu, T. Ljubicic, W.J. Llope, H. Long, R.S. Longacre, W.A. Love, Y. Lu, T. Ludlam, D. Lynn, G.L. Ma, J.G. Ma, Y.G. Ma, D. Magestro, D.P. Mahapatra, R. Majka, L.K. Mangotra, R. Manweiler, S. Margetis, C. Markert, L. Martin, H.S. Matis, Yu.A. Matulenko, C.J. McClain, T.S. McShane, Yu. Melnick, A. Meschanin, J. Millane, M.L. Miller, N.G. Minaev, S. Mioduszewski, C. Mironov, A. Mischke, D.K. Mishra, J. Mitchell, B. Mohanty, L. Molnar, C.F. Moore, D.A. Morozov, M.G. Munhoz, B.K. Nandi, C. Nattrass, T.K. Nayak, J.M. Nelson, P.K. Netrakanti, L.V. Nogach, S.B. Nurushev, G. Odyniec, A. Ogawa, V. Okorokov, M. Oldenburg, D. Olson, M. Pachr, S.K. Pal, Y. Panebratsev, S.Y. Panitkin, A.I. Pavlinov, T. Pawlak, T. Peitzmann, V. Perevoztchikov, C. Perkins, W. Peryt, S.C. Phatak, R. Picha, M. Planinic, J. Pluta, N. Poljak, N. Porile, J. Porter, A.M. Poskanzer, M. Potekhin, E. Potrebenikova, B.V.K.S. Potukuchi, D. Prindle, C. Pruneau, J. Putschke, G. Rakness, R. Raniwala, S. Raniwala, R.L. Ray, S.V. Razin, J. Reinnarth, D. Relyea, F. Retiere, A. Ridiger, H.G. Ritter, J.B. Roberts, O.V. Rogachevskiy, J.L. Romero, A. Rose, C. Roy, L. Ruan, M.J. Russcher, R. Sahoo, T. Sakuma, S. Salur, J. Sandweiss, M. Sarsour, P.S. Sazhin, J. Schambach, R.P. Scharenberg, N. Schmitz, K. Schweda, J. Seger, I. Selyuzhenkov, P. Seyboth, A. Shabetai, E. Shahaliev, M. Shao, M. Sharma, W.Q. Shen, S.S. Shimanskiy, E. Sichtermann, F. Simon, R.N. Singaraju, N. Smirnov, R. Snellings, G. Sood, P. Sorensen, J. Sowinski, J. Speltz, H.M. Spinka, B. Srivastava, A. Stadnik, T.D.S. Stanislaus, R. Stock, A. Stolpovsky, M. Strikhanov, B. Stringfellow, A.A.P. Suaide, E. Sugarbaker, M. Sumbera, Z. Sun, B. Surrow, M. Swanger, T.J.M. Symons, A. Szanto de Toledo, A. Tai, J. Takahashi, A.H. Tang, T. Tarnowsky, D. Thein, J.H. Thomas, A.R. Timmins, S. Timoshenko, M. Tokarev, T.A. Trainor, S. Trentalange, R.E. Tribble, O.D. Tsai, J. Ulery, T. Ullrich, D.G. Underwood, G. Van Buren, N. van der Kolk, M. van Leeuwen, A.M. Vander Molen, R. Varma, I.M. Vasilevski, A.N. Vasiliev, R. Vernet, S.E. Vigdor, Y.P. Viyogi, S. Vokal, S.A. Voloshin, W.T. Waggoner, F. Wang, G. Wang, J.S. Wang, X.L. Wang, Y. Wang, J.W. Watson, J.C. Webb, G.D. Westfall, A. Wetzler, C. Whitten, H. Wieman, S.W. Wissink, R. Witt, J. Wood, J. Wu, N. Xu, Q.H. Xu, Z. Xu, P. Yepes, I.-K. Yoo, V.I. Yurevich, W. Zhan, H. Zhang, W.M. Zhang, Y. Zhang, Z.P. Zhang, Y. Zhao, C. Zhong, R. Zoulkarneev, Y. Zoulkarneeva, A.N. Zubarev, and J.X. Zuo
Subjects: Nuclear and High Energy Physics
Published: 2014

25. Dissociation des réponses cardiorespiratoires et neuromusculaires à l’exercice à la suite de 4 semaines de réentraînement à l’effort chez des patients cardiaques

Author: P.-M. Leprêtre, L. Gaillard, Thierry Weissland, S. Delanaud, M. Ghannem, T. Porcher, A. Barnabé, N. Jaunet, Adaptations Physiologiques à l'Exercice et Réadaptation à l'effort - UR UPJV 3300 (APERE), CHU Amiens-Picardie-Université de Picardie Jules Verne (UPJV), Biomécanique et génie biomédical (BIM), Centre National de la Recherche Scientifique (CNRS), Centre interuniversitaire de recherche et d'ingenierie des matériaux (CIRIMAT), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)
Subjects: Gynecology, 03 medical and health sciences, medicine.medical_specialty, 0302 clinical medicine, business.industry, medicine, [SDV.MHEP.PHY]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO], 030229 sport sciences, 030204 cardiovascular system & hematology, Cardiology and Cardiovascular Medicine, business, ComputingMilieux_MISCELLANEOUS
Abstract: Resume Un programme court de reentrainement a l’effort (RE) en readaptation cardiovasculaire peut induire une dissociation des reponses du pic de consommation d’oxygene (VO2pic) et de puissance maximale toleree (PMT) ; soit des adaptations cardiorespiratoires et neuromusculaires differentes a l’effort. Objectif de l’etude Examiner les effets d’un court RE sur des indices de reponses cardiorespiratoires (VO2pic) et musculaires (RMS de l’activite electromyographique de surface du vastus lateralis) chez le patient cardiaque. Patients et methode Neuf sujets (64,0 ± 3,1 ans, 172,9 ± 4,8 cm, 83,4 ± 16,3 kg, IMC : 27,83 ± 4,5) ont realise un CPX sur ergocycle, avant et apres quatre semaines de RE. SV1 correspondait a la cassure dans la relation dioxyde de carbone exhale (CO2) – consommation d’oxygene ; SV2 a l’intensite pour laquelle l’equivalent respiratoire en CO2 augmentait. EMGs1 et EMGs2 etaient definis comme les premier et second points de rupture dans la relation RMS – intensite d’exercice. Resultats Le RE a induit des augmentations significatives de VO2pic (+3,5 ± 2,9 mL min−1 kg−1), PMT, SV1 et SV2 (p Conclusion Le reentrainement a l’effort peut induire des adaptations cardiorespiratoires et neuromusculaires differentes d’ou l’importance d’apprehender, des le debut de prise en charge, les facteurs limitatifs de chaque patient cardiaque pour leur prescrire les activites physiques adaptees les mieux adaptees afin d’optimiser leur etat de sante.
Published: 2017

26. [Short-term exercise-based cardiac rehabilitation induced changes in cardiorespiratory, mechanical and neuromuscular responses to progressive exercise testing]

Author: P-M, Leprêtre, M, Ghannem, S, Delanaud, N, Jaunet, L, Gaillard, A, Barnabé, T, Porcher, and T, Weissland
Subjects: Cardiac Rehabilitation, Electromyography, Respiration, Exercise Test, Humans, Heart, Middle Aged, Biomechanical Phenomena, Exercise Therapy, Quadriceps Muscle
Abstract: Previous studies showed that changes in peak of oxygen uptake value (VOThe aim of the study was to compare the responses to exercise training of electromyographic activities of vastus lateralis (rms-EMG) and respiratory parameters.Nine cardiac patients (64.0±3.1y, 172.9±4.8cm, 83.4±16.3kg, BMI: 27.8±4.5) performed an incremental cycling exercise test to determine MTP, VOShort-term exercise training (23.7±8.8 days) induced a significant increase in VOThis might be taken into account for prescribing exercise rehabilitation according initial clinical limitations of patients.
Published: 2017

27. Control of structure-specific endonucleases to maintain genome stability

Author: Pierre-Marie Dehé, Pierre-Henri L. Gaillard, Centre de Recherche en Cancérologie de Marseille (CRCM), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Aix Marseille Université (AMU), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC), Aix Marseille Université (AMU), Institut National de la Santé et de la Recherche Médicale (INSERM), and Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, DNA Replication, Xeroderma pigmentosum, DNA Repair, Flap structure-specific endonuclease 1, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Genomic Instability, Nucleic acid secondary structure, 03 medical and health sciences, Endonuclease, Nuclear Matrix-Associated Proteins, Transcription (biology), medicine, Holliday junction, Animals, Humans, Molecular Biology, Genetics, biology, Cell Cycle, DNA replication, [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Cell Biology, medicine.disease, Endonucleases, MUS81, 030104 developmental biology, [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics, biology.protein
Abstract: International audience; Structure-specific endonucleases (SSEs) have key roles in DNA replication, recombination and repair, and emerging roles in transcription. These enzymes have specificity for DNA secondary structure rather than for sequence, and therefore their activity must be precisely controlled to ensure genome stability. In this Review, we discuss how SSEs are controlled as part of genome maintenance pathways in eukaryotes, with an emphasis on the elaborate mechanisms that regulate the members of the major SSE families — including the xeroderma pigmentosum group F-complementing protein (XPF) and MMS and UV-sensitive protein 81 (MUS81)-dependent nucleases, and the flap endonuclease 1 (FEN1), XPG and XPG-like endonuclease 1 (GEN1) enzymes — during processes such as DNA adduct repair, Holliday junction processing and replication stress. We also discuss newly characterized connections between SSEs and other classes of DNA-remodelling enzymes and cell cycle control machineries, which reveal the importance of SSE scaffolds such as the synthetic lethal of unknown function 4 (SLX4) tumour suppressor for the maintenance of genome stability.
Published: 2017

28. PNS52 ASSESSMENT OF THE IMPACT OF THE LEVEL OF ASMR ON THE DRUG'S PRICE AND ITS NEGOTIATION TIME WITH THE HEALTH AUTHORITIES IN TREATMENTS THAT BENEFITED FROM THE FRENCH EARLY ACCESS PROGRAM

Author: L. Gaillard and G. Leiba
Subjects: Negotiation, Health Policy, media_common.quotation_subject, Public Health, Environmental and Occupational Health, Business, Marketing, media_common
Published: 2019

29. Regulation of Mus81–Eme1 Holliday junction resolvase in response to DNA damage

Author: Bertrand Llorente, Pierre-Henri L. Gaillard, Pierre-Marie Dehé, Arato Takedachi, Stéphane Coulon, Paul Shanahan, John R. Yates, Sarah Scaglione, James A. Wohlschlegel, Paul Russell, Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), The Scripps Research Institute [La Jolla], University of California [San Diego] (UC San Diego), University of California-University of California, Department of Surgical and Radiological Sciences, University of California [Davis] (UC Davis), Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC), Aix Marseille Université (AMU), Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS), The Scripps Research Institute [La Jolla, San Diego], University of California (UC)-University of California (UC), and gaillard, pierre-henri
Subjects: Genome instability, DNA damage, Cell Cycle Proteins, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], [SDV.GEN.GH] Life Sciences [q-bio]/Genetics/Human genetics, [SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Models, Biological, DNA-binding protein, Article, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, [SDV.CAN] Life Sciences [q-bio]/Cancer, Structural Biology, Gene Expression Regulation, Fungal, CDC2 Protein Kinase, Schizosaccharomyces, [SDV.BC.BC] Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Holliday junction, Phosphorylation, Molecular Biology, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Genetics, 0303 health sciences, biology, Holliday Junction Resolvases, DNA replication, Helicase, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], Endonucleases, MUS81, Cell biology, DNA-Binding Proteins, Checkpoint Kinase 2, [SDV.BBM.MN] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics, chemistry, biology.protein, Schizosaccharomyces pombe Proteins, Protein Kinases, Protein Processing, Post-Translational, 030217 neurology & neurosurgery, DNA, DNA Damage, Signal Transduction
Abstract: Structure-specific DNA endonucleases have critical roles during DNA replication, repair and recombination, yet they also harbor the potential for causing genome instability. Controlling these enzymes may be essential to ensure efficient processing of ad hoc substrates and to prevent random, unscheduled processing of other DNA structures, but it is unknown whether structure-specific endonucleases are regulated in response to DNA damage. Here, we uncover DNA damage-induced activation of Mus81-Eme1 Holliday junction resolvase in fission yeast. This novel regulation requires both Cdc2CDK1 and Rad3ATR-dependent phosphorylations of Eme1. Mus81-Eme1 activation prevents gross chromosomal rearrangements in cells lacking the BLM-related DNA helicase Rqh1. We propose that linking Mus81-Eme1 DNA damaged-induced activation to cell cycle progression ensures efficient resolution of Holliday junctions that escape dissolution by Rqh1-TopIII while preventing unnecessary DNA cleavages.
Published: 2013

30. Impaired Frontal-Limbic White Matter Maturation in Children at Risk for Major Depression

Author: Joseph Biederman, Susan Whitfield-Gabrieli, Mai Uchida, John D. E. Gabrieli, Pavel Yarmak, Xiaoqian J. Chai, Oliver Doehrmann, Dina R. Hirshfeld-Becker, Michelle Han, Zeynep M. Saygin, Yuwen Hung, Tara Kenworthy, and Schuyler L. Gaillard
Subjects: Male, Genu of the corpus callosum, Adolescent, Cognitive Neuroscience, Physiology, Corpus callosum, 050105 experimental psychology, Corpus Callosum, White matter, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, Fractional anisotropy, History of depression, Cingulum (brain), Medicine, Humans, 0501 psychology and cognitive sciences, Risk factor, Child, Depression (differential diagnoses), Depressive Disorder, Major, business.industry, 05 social sciences, Original Articles, White Matter, Affect, medicine.anatomical_structure, Diffusion Magnetic Resonance Imaging, Diffusion Tensor Imaging, Anisotropy, Female, Nerve Net, business, 030217 neurology & neurosurgery
Abstract: Depression is among the most common neuropsychiatric disorders. It remains unclear whether brain abnormalities associated with depression reflect the pathological state of the disease or neurobiological traits predisposing individuals to depression. Parental history of depression is a risk factor that more than triples the risk of depression. We compared white matter (WM) microstructure cross-sectionally in 40 children ages 8-14 with versus without parental history of depression (At-Risk vs. Control). There were significant differences in age-related changes of fractional anisotropy (FA) between the groups, localized in the anterior fronto-limbic WM pathways, including the anterior cingulum and the genu of the corpus callosum. Control children exhibited typical increasing FA with age, whereas At-Risk children exhibited atypical decreasing FA with age in these fronto-limbic regions. Furthermore, dorsal cingulate FA significantly correlated with depressive symptoms for At-Risk children. The results suggest maturational WM microstructure differences in mood-regulatory neurocircuitry that may contribute to neurodevelopmental risk for depression. The study provides new insights into neurodevelopmental susceptibility to depression and related disabilities that may promote early preventive intervention approaches.
Published: 2016

31. Near-side azimuthal and pseudorapidity correlations using neutral strange baryons and mesons ind+Au, Cu + Cu, and Au + Au collisions atsNN=200GeV

Author: N. Xu, P. Filip, D. G. Underwood, Q. Yang, Jorn Henning Putschke, J. Cheng, L. C. De Silva, A. V. Brandin, P. Pile, G. D. Westfall, K. Xin, R. Witt, W. Guryn, D. L. Olvitt, Xiangming Sun, Vipul Bairathi, Roli Esha, Jie Zhao, T. Lin, S. Horvat, M. Simko, James Brandenburg, W. Zha, A. Sarkar, J. Fedorisin, R. Vertesi, Q. H. Xu, S. Mioduszewski, J. Xu, W. J. Llope, J. Porter, L. Yi, F. Liu, H. Z. Huang, S. Vokal, N. K. Pruthi, Lee Stuart Barnby, J. Engelage, G. Odyniec, D. Arkhipkin, Donald M. McDonald, Siwei Luo, A. Hamed, Y. Zoulkarneeva, J. K. Adkins, C. Zhong, E. C. Aschenauer, S. Margetis, G. Nigmatkulov, Alexander Jentsch, Prithwish Tribedy, B. K. Srivastava, P. V. Shanmuganathan, T. J. Humanic, R. Fatemi, L. He, Xiaofeng Luo, Guo-Liang Ma, G. Eppley, Ivan Kisel, K. Yip, B. Huang, K. Krueger, Jaroslav Bielcik, J. S. Wang, Shangfeng Yang, J. M. Landgraf, Zhigang Xiao, X. Dong, Mukesh Sharma, T. Huang, G. Wang, Zongye Zhang, R. R. Debbe, J. Jia, Arka Chatterjee, M. Calderon De La Barca Sanchez, A. I. Hamad, J. H. Chen, L. E. Dunkelberger, A. Attri, H. G. Ritter, M. Strikhanov, M. Vandenbroucke, R. D. Majka, Y. Guo, Michal Sumbera, Rafal Sikora, J. Pluta, A. S. Hirsch, W. Li, Rene Bellwied, D. A. Morozov, J. C. Dunlop, L. Wen, J. Sandweiss, L. Gaillard, R. E. Tribble, R. S. Longacre, Jongmin Lee, R. L. Ray, M.U. Ashraf, Xiang Li, N. G. Minaev, Guannan Xie, Xuan Zhang, I. G. Bordyuzhin, M. Cherney, H. Qiu, Nasim, A. A. Derevschikov, O. V. Rogachevskiy, H. J. Jang, M. K. Mustafa, Y. Li, S. Singha, R. Ma, P. Kravtsov, A. M. Schmah, T. Ljubicic, Sergey Voloshin, J. Schambach, F. Videbæk, Christine Nattrass, Anju Bhasin, L. C. Bland, M. Tokarev, Yang Yang, G. Igo, Lokesh Kumar, J. Novak, G. Webb, B. Pawlik, Gerald W Hoffmann, J. Rusnak, Z. J. Sun, Hua Pei, S. K. Tripathy, Hanna Paulina Zbroszczyk, Abdel Nasser Tawfik, A. F. Kraishan, R. Varma, I. Sakrejda, B. Sharma, Niseem Magdy, K. Jiang, H. Caines, Zubayer Ahammed, Jana Bielcikova, A. Quintero, Sukalyan Chattopadhyay, J. Bouchet, L. Ruan, T. Niida, B. J. Summa, K. Poniatowska, A. Lebedev, A. Gibson, Yanjun Wu, S. K. Gupta, Giacomo Contin, Q. Y. Shou, Yi-Fei Xu, Basanta Kumar Nandi, P. Seyboth, E. P. Sichtermann, K. D. Landry, J. Butterworth, D. N. Svirida, S. W. Wissink, Y. Zhang, R. Pak, J. L. Drachenberg, T. Todoroki, Olga Evdokimov, Nihar Sahoo, Z. Shi, B. S. Page, D. P. Kikola, Z. H. Khan, W. Solyst, P. Sorensen, X. C. Chen, P. Huck, Z. Chang, T. D. S. Stanislaus, V. A. Okorokov, Madan M. Aggarwal, Peter Graham Jones, Fuqiang Wang, Anthony Robert Timmins, Z. Feng, Jay Roberts, B. Stringfellow, J. B. Zhang, D. K. Mishra, D. D. Koetke, J. E. Draper, Leszek Adamczyk, T. Ullrich, H. H. Wieman, X. Bai, Adam Ryszard Kisiel, Nilay Shah, M. Lomnitz, C-Q. Li, H. W. Ke, P. Federic, Bedangadas Mohanty, H. J. Crawford, Z. W. Miller, S. Ramachandran, O. Eyser, B. Di Ruzza, C. M. Du, H. S. Xu, J. M. Campbell, Jian Deng, R. P. Scharenberg, Christina Markert, L. Didenko, I. Chakaberia, A. Manion, D. Kalinkin, M. J. Skoby, M. M. Mondal, D. Smirnov, D. Tlusty, L. Fulek, G. Agakishiev, D. S. Gunarathne, A. M. Poskanzer, Z. Ye, O. Rusnakova, H. S. Matis, M. A.C. Lamont, S. Y. Noh, W. Christie, A. Vossen, M. Posik, T. G. Dedovich, X. Huang, A. Kechechyan, D. Cebra, M. R. Girard, I. K. Yoo, L. K. Kosarzewski, R. Haque, Maksym Zyzak, W. W. Jacobs, C. E. Flores, E. G. Judd, S. Kabana, Pradip Kumar Sahu, H. M. Spinka, Andrey Vasiliev, J. Lauret, Yu-Gang Ma, P. Chaloupka, Z. J. Xu, P. Bhattarai, Isaac Upsal, Y. Fisyak, J. C. Webb, J. Y. Zhang, Lin Ma, N. Yu, Kejun Kang, K. Oh, S. Trentalange, L. V. Nogach, I. G. Alekseev, Sumit R. Das, R. Lednicky, G. Van Nieuwenhuizen, M. A. Lisa, J. C. Mei, L. Kochenda, Janet Elizabeth Seger, T. Tarnowsky, J. H. Thomas, Bernd Surrow, Y. J. Sun, L. P. Zhou, C. Dilks, S. Fazio, B. I. Abelev, M. Stepanov, G. S. Averichev, W. B. Schmidke, C. A. Gagliardi, Haiyan Wang, J. Thäder, C. Yang, Xiaofeng Zhu, K. Kauder, S. B. Nurushev, A. Ogawa, M. Shao, Y. X. Pan, G. Van Buren, S. S. Shi, Marek Bombara, J. L. Romero, J. W. Harris, A. H. Tang, I. Bunzarov, Wei Xie, A. Aparin, Y. Pandit, N. Schmitz, E. Shahaliev, Anik Gupta, Frank Jm Geurts, K. Meehan, S. H. Zhang, A. Sharma, S. McKinzie, Zebo Tang, Leo Clifford Greiner, C. Perkins, O. D. Tsai, L. Song, W. Q. Shen, D. Garand, Sevil Salur, Y. Wang, A. K. Bhati, D. Keane, Nikolai Smirnov, R. Stock, L. G. Efimov, T. K. Nayak, Y. Panebratsev, D. Grosnick, and Samuel Heppelmann
Subjects: Physics, Strange quark, Particle physics, Meson, 010308 nuclear & particles physics, Branching fraction, Hadron, Hyperon, Elementary particle, 01 natural sciences, Charged particle, Nuclear physics, Pseudorapidity, 0103 physical sciences, 010306 general physics
Abstract: Author(s): Abelev, B; Adamczyk, L; Adkins, JK; Agakishiev, G; Aggarwal, MM; Ahammed, Z; Alekseev, I; Aparin, A; Arkhipkin, D; Aschenauer, EC; Ashraf, MU; Attri, A; Averichev, GS; Bai, X; Bairathi, V; Barnby, LS; Bellwied, R; Bhasin, A; Bhati, AK; Bhattarai, P; Bielcik, J; Bielcikova, J; Bland, LC; Bombara, M; Bordyuzhin, IG; Bouchet, J; Brandenburg, JD; Brandin, AV; Bunzarov, I; Butterworth, J; Caines, H; Calderon De La Barca Sanchez, M; Campbell, JM; Cebra, D; Chakaberia, I; Chaloupka, P; Chang, Z; Chatterjee, A; Chattopadhyay, S; Chen, JH; Chen, X; Cheng, J; Cherney, M; Christie, W; Contin, G; Crawford, HJ; Das, S; De Silva, LC; Debbe, RR; Dedovich, TG; Deng, J; Derevschikov, AA; Di Ruzza, B; Didenko, L; Dilks, C; Dong, X; Drachenberg, JL; Draper, JE; Du, CM; Dunkelberger, LE; Dunlop, JC; Efimov, LG; Engelage, J; Eppley, G; Esha, R; Evdokimov, O; Eyser, O; Fatemi, R; Fazio, S; Federic, P; Fedorisin, J; Feng, Z; Filip, P; Fisyak, Y; Flores, CE; Fulek, L; Gagliardi, CA; Gaillard, L; Garand, D; Geurts, F; Gibson, A; Girard, M; Greiner, L; Grosnick, D; Gunarathne, DS | Abstract: We present measurements of the near side of triggered di-hadron correlations using neutral strange baryons (Λ,Λ) and mesons (KS0) at intermediate transverse momentum (3 l pT l6 GeV/c) to look for possible flavor and baryon-meson dependence. This study is performed in d+Au, Cu+Cu, and Au+Au collisions at sNN=200 GeV measured by the STAR experiment at RHIC. The near-side di-hadron correlation contains two structures, a peak which is narrow in azimuth and pseudorapidity consistent with correlations from jet fragmentation, and a correlation in azimuth which is broad in pseudorapidity. The particle composition of the jet-like correlation is determined using identified associated particles. The dependence of the conditional yield of the jet-like correlation on the trigger particle momentum, associated particle momentum, and centrality for correlations with unidentified trigger particles are presented. The neutral strange particle composition in jet-like correlations with unidentified charged particle triggers is not well described by PYTHIA. However, the yield of unidentified particles in jet-like correlations with neutral strange particle triggers is described reasonably well by the same model.
Published: 2016

32. [Is there a disassociation of ventilatory and electromyographic thresholds in patients with heart disease during a graded cycling exercise?]

Author: P-M, Leprêtre, M, Ghannem, S, Delanaud, T, Porcher, A, Barnabé, L, Gaillard, N, Jaunet, and T, Weissland
Subjects: Male, Oxygen, Anaerobic Threshold, Electromyography, Muscle Fatigue, Exercise Test, Humans, Female, Carbon Dioxide, Middle Aged, Pulmonary Ventilation, Aged, Quadriceps Muscle
Abstract: Exercise prescription was generally based on the determination of ventilatory thresholds (VTTo observe the occurrence of EMGtThirty-four CP (62.1±7.3years, 172.1±6.3cm, 81.3±15.3kg, BMI: 27.3±4.1) performed a cycle CPX at a 60-rpm cadence. VTPeak values of oxygen uptake (16.3±4.6mL·minThe EMG
Published: 2016

33. Isolation and characterization of polymorphic microsatellite loci for the gopher tortoise (Gopherus polyphemus) and cross-amplification in other species of Gopherus

Author: Brian R. Kreiser, Carl P. Qualls, Daniel L. Gaillard, and Joshua R. Ennen
Subjects: Conservation genetics, Loss of heterozygosity, biology, Tortoise, Polyphemus, Genetics, Microsatellite, Zoology, biology.organism_classification, Cross amplification, Gopherus flavomarginatus, Ecology, Evolution, Behavior and Systematics, Gopherus berlandieri
Abstract: All members of the tortoise genus Gopherus have some degree of conservation concern. We report on the development of thirty-two new microsatellite loci for gopher tortoise, which were tested on 31 individuals from Ft. Benning, Georgia, USA. These loci possessed an average number of 5.09 alleles, an average observed heterozygosity of 0.574 and an average expected heterozygosity of 0.589. We also tested these loci in three congeners: Gopherus morafkai, Gopherus flavomarginatus and Gopherus berlandieri. The new microsatellite loci should prove useful in conservation efforts of Gopherus polyphemus and other Gopherus species.
Published: 2012

34. Mitochondrial DNA Assessment of the Phylogeography of the Gopher Tortoise

Author: Brian R. Kreiser, Matthew J. Aresco, Aaron W. Schrey, Thomas W. ″Bill″ Hentges, Daniel L. Gaillard, Roger D. Birkhead, Tracey D. Tuberville, Henry R. Mushinsky, Carl P. Qualls, Joshua R. Ennen, and Earl D. McCoy
Subjects: Conservation genetics, education.field_of_study, Ecology, Tortoise, Range (biology), Population, Zoology, Biology, Phylogeography, Genetic structure, Threatened species, Animal Science and Zoology, education, Genetic isolate, Ecology, Evolution, Behavior and Systematics, Nature and Landscape Conservation
Abstract: Identifying geographic barriers that define genetic structure within a species is crucial in formulating an effective conservation plan. The identification of appropriate management units is critical for the protection and recovery of the gopher tortoise Gopherus polyphemus, which have declined across their entire range. Previous molecular work at various spatial scales has identified distinct population assemblages of the gopher tortoise. The goal of this study was to assess the genetic structure in gopher tortoises through a more complete sampling of the federally listed as threatened portion of the range and evaluate the extent of genetic isolation imposed by several potential geographic barriers. We sequenced a 712–base-pair portion of a mitochondrial gene (NADH dehydrogenase 4) for 322 individuals from 42 sites across the range. We found two major assemblages of haplotypes separated by a modest phylogenetic break (average uncorrected p distance = 0.015). The biogeographic barrier that best...
Published: 2012

35. Disruption of mouse Slx4, a regulator of structure-specific nucleases, phenocopies Fanconi anemia

Author: Gerry P. Crossan, Mikko I. Kettunen, Frederic Langevin, Mark J. Arends, David Y. Lewis, Sanger Mouse Genetics, Iván V. Rosado, David J. Adams, Louise van der Weyden, Pierre-Henri L. Gaillard, Ferdia A. Gallagher, Ketan J. Patel, Kevin M. Brindle, Rebecca E. McIntyre, Medical Research Council, The Wellcome Trust Sanger Institute [Cambridge], Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC), Centre de Recherche en Cancérologie de Marseille (CRCM / U891 Inserm), Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Cancer Research UK Cambridge Institute [Cambridge, Royaume-Uni] (CRUK), University of Cambridge [UK] (CAM), Addenbrooke's Hospital, and Cambridge University NHS Trust
Subjects: Male, congenital, hereditary, and neonatal diseases and abnormalities, Fanconi anemia, complementation group C, DNA damage, Regulator, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Biology, Article, Recombinases, Mice, 03 medical and health sciences, 0302 clinical medicine, Fanconi anemia, hemic and lymphatic diseases, Genetics, medicine, Recombinase, Animals, Humans, Gene, Cellular Senescence, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Mice, Knockout, Phenocopy, 0303 health sciences, Genetic Complementation Test, nutritional and metabolic diseases, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], Fibroblasts, Hematopoietic Stem Cells, medicine.disease, Magnetic Resonance Imaging, Cross-Linking Reagents, Fanconi Anemia, [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics, 030220 oncology & carcinogenesis, Female, Cell aging, DNA Damage
Abstract: The evolutionarily conserved SLX4 protein, a key regulator of nucleases, is critical for DNA damage response. SLX4 nuclease complexes mediate repair during replication and can also resolve Holliday junctions formed during homologous recombination. Here we describe the phenotype of the Btbd12 knockout mouse, the mouse ortholog of SLX4, which recapitulates many key features of the human genetic illness Fanconi anemia. Btbd12-deficient animals are born at sub-Mendelian ratios, have greatly reduced fertility, are developmentally compromised and are prone to blood cytopenias. Btbd12(-/-) cells prematurely senesce, spontaneously accumulate damaged chromosomes and are particularly sensitive to DNA crosslinking agents. Genetic complementation reveals a crucial requirement for Btbd12 (also known as Slx4) to interact with the structure-specific endonuclease Xpf-Ercc1 to promote crosslink repair. The Btbd12 knockout mouse therefore establishes a disease model for Fanconi anemia and genetically links a regulator of nuclease incision complexes to the Fanconi anemia DNA crosslink repair pathway.
Published: 2011

36. Structured Didactic Education Program for Writing Case Reports Can Motivate Medical Students

Author: Yosuke Kakisaka, Mayu Fujikawa, and Schuyler L. Gaillard
Subjects: lcsh:R5-920, Report writing, Case writing, business.industry, lcsh:Public aspects of medicine, Diagnostic reasoning, lcsh:RA1-1270, Structured education, Nothing, ComputingMilieux_COMPUTERSANDEDUCATION, Mathematics education, Medicine, na, Clinical case, lcsh:Medicine (General), business, Educational program
Abstract: As we described in our editorial in this issue, writing case reports is crucial in both building case writing skills and enhancing clinical diagnostic reasoning. Case reports have always been an integral part of medical history. However, case reporting instruction has previously tended to be vague, whereas students may need more detailed support such as step-by-step guidance. To address this issue, we recently launched an educational program, entitled “How to write a case report: Visualizing the trajectory from nothing to submission.” The program consists of two parts: (1) a didactic lecture on the theoretical principles of case-writing, and (2) hands-on case report writing practice using a clinical case. The program is suitable for entry-level writers intending to make a submission to a journal. The present article introduces the contents of the didactic part of our program and evaluates the motivational level of medical students towards case writing. The feasibility of structured education in case writing will also be discussed.
Published: 2016

37. Human SLX4 Is a Holliday Junction Resolvase Subunit that Binds Multiple DNA Repair/Recombination Endonucleases

Author: Pierre-Henri L. Gaillard, Charly Chahwan, Ewan R. Taylor, Samira Fekairi, Stéphane Coulon, Paul Russell, Sarah Scaglione, Cristian I. Ruse, Clare H. McGowan, Agnès Tissier, John R. Yates, Meng-Qiu Dong, Robert P. P. Fuchs, Instabilité du génome et cancérogénèse (UPR3081), Centre National de la Recherche Scientifique (CNRS), Centre de Recherche en Cancérologie de Marseille (CRCM / U891 Inserm), Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), The Scripps Research Institute [La Jolla], University of California [San Diego] (UC San Diego), and University of California-University of California
Subjects: DNA Repair, PROTEINS, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Article, Chromosomes, General Biochemistry, Genetics and Molecular Biology, Homing endonuclease, 03 medical and health sciences, 0302 clinical medicine, Holliday junction, Animals, Humans, Site-specific recombination, Flap endonuclease, Endodeoxyribonucleases, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Recombination, Genetic, Genetics, DNA, Cruciform, 0303 health sciences, biology, Biochemistry, Genetics and Molecular Biology(all), Holliday Junction Resolvases, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], DNA, MUS81, Cell biology, [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics, 030220 oncology & carcinogenesis, biology.protein, Nucleic Acid Conformation, Homologous recombination, Flap endonuclease activity
Abstract: SummaryStructure-specific endonucleases resolve DNA secondary structures generated during DNA repair and recombination. The yeast 5′ flap endonuclease Slx1-Slx4 has received particular attention with the finding that Slx4 has Slx1-independent key functions in genome maintenance. Although Slx1 is a highly conserved protein in eukaryotes, no orthologs of Slx4 were reported other than in fungi. Here we report the identification of Slx4 orthologs in metazoa, including fly MUS312, essential for meiotic recombination, and human BTBD12, an ATM/ATR checkpoint kinase substrate. Human SLX1-SLX4 displays robust Holliday junction resolvase activity in addition to 5′ flap endonuclease activity. Depletion of SLX1 and SLX4 results in 53BP1 foci accumulation and H2AX phosphorylation as well as cellular hypersensitivity to MMS. Furthermore, we show that SLX4 binds the XPFERCC4 and MUS81 subunits of the XPF-ERCC1 and MUS81-EME1 endonucleases and is required for DNA interstrand crosslink repair. We propose that SLX4 acts as a docking platform for multiple structure-specific endonucleases.
Published: 2009

38. Energy and system size dependence of ϕ meson production in Cu+Cu and Au+Au collisions

Author: M. Shao, G. Van Buren, L. Kotchenda, S. Hepplemann, Y. Panebratsev, D. Grosnick, V. Kouchpil, M. Heinz, Lee Stuart Barnby, J. C. Dunlop, S. S. Shi, Jaroslav Bielcik, J. S. Wang, T. W. Ludlam, Zubayer Ahammed, M. J. LeVine, M. R. Dutta Mazumdar, O. V. Rogachevskiy, Rolf K. Reed, A. Ridiger, W. Christie, J. M. Nelson, L. K. Mangotra, W. M. Zhang, V. Ghazikhanian, W. A. Love, D. D. Koetke, G. Lin, Anik Gupta, Y. G. Ma, W. Peryt, Marek Bombara, C. Nepali, M. J. Skoby, E. Elhalhuli, Jifa Tian, R. Manweiler, Hongfang Liu, J. L. Drachenberg, T. S. McShane, W. T. Waggoner, S. H. Zhang, Andrey Vasiliev, Jorn Henning Putschke, A. V. Brandin, A. Feng, Jay Roberts, A. M. Poskanzer, B. E. Bonner, O. D. Tsai, David Jonathan Hofman, M. Elnimr, M. C. Cervantes, A. Kechechyan, Andre Mischke, A. Ogawa, H. F. Chen, Thomas A. Trainor, W. R. Edwards, J. H. Thomas, A. Stadnik, Peter Martin Jacobs, M. Krus, R. Cendejas, C-Q. Li, R. P. Scharenberg, Christina Markert, J. Takahashi, D. Kettler, H. S. Matis, R. Witt, D. Thein, Sevil Salur, S. Mioduszewski, J. W. Harris, Raimond Snellings, H. Okada, S. B. Nurushev, S. Baumgart, Matthew Walker, V. Yu Khodyrev, R. N. Singaraju, I. Selyuzhenkov, Vitaly Okorokov, W. W. Jacobs, G. S. Averichev, J. Lauret, F. Videbæk, Sukalyan Chattopadhyay, S. Bharadwaj, B. K. Srivastava, I. M. Vasilevski, W. J. Llope, J. L. Romero, K. Kajimoto, E. Finch, Y. Li, S. M. Guertin, M. Kopytine, R. Lednicky, P. Seyboth, E. J. Garcia-Solis, M. Tokarev, Boris Hippolyte, V. Emelianov, A. K. Bhati, D. Keane, Nikolai Smirnov, S. E. Vigdor, Christian Claude Kuhn, A. P. Meschanin, Bernd Surrow, M. S. Ganti, P. Yepes, A. H. Tang, Xiangming Sun, D. P. Mahapatra, R. S. Hollis, Janet Elizabeth Seger, A. M. Hoffman, A. Szanto de Toledo, J. Kapitan, J. Mitchell, L. G. Efimov, Y. J. Sun, S. Kabana, Y. P. Viyogi, A. R. Timmins, S. M. Dogra, D. Tlusty, Mirko Planinic, Pawan Kumar Netrakanti, M. Zawisza, H. M. Spinka, Morton Kaplan, X. Z. Cai, C-H. Lee, T. K. Nayak, S. U. Chung, T. D. S. Stanislaus, C. A. Gagliardi, R. Derradi de Souza, B. I. Abelev, M. Calderon De La Barca Sanchez, Y. N. Gorbunov, J. E. Draper, O. Catu, O. I. Mall, H. H. Wieman, H. Zhang, T. Pawlak, Y. P. Lu, V. Perevoztchikov, R. S. Longacre, Bedangadas Mohanty, A. Kocoloski, S. Dash, T. J. Humanic, M. A.C. Lamont, B. D. Anderson, I.A. Savin, I. Sakrejda, Debasish Das, Raghunath Sahoo, Marcelo Gameiro Munhoz, D. J. Prindle, M. J. M. Codrington, N. K. Pruthi, J. X. Zuo, Madan M. Aggarwal, Fuqiang Wang, A. M. Vander Molen, F. Liu, Sergey Voloshin, G. Igo, Christine Nattrass, Guo-Liang Ma, G. Eppley, X. Lin, Qian Yue, M. Bystersky, J. Bouchet, S. LaPointe, A. Gordon, B. V.K.S. Potukuchi, E. Hjort, Y. F. Wu, J. Pluta, J. Engelage, R. Millner, J. P. Coffin, Jie Liu, W. L. Zhan, Z. P. Zhang, R. Varma, Premomoy Ghosh, Yu A. Matulenko, B. Grube, B. S. Page, S. Timoshenko, A. S. Hirsch, J. C. Webb, P. Kravtsov, Frank Simon, Andrew Rose, Y. Zhao, D. P. Kikola, I. K. Yoo, P. Fachini, J. M. Landgraf, M. Pachr, Hanna Paulina Zbroszczyk, R. E. Tribble, C. L. Jones, R. D. Majka, K. S.F.F. Guimaraes, J. Sowinski, M. A. Lisa, M. Strikhanov, D. R. Beavis, Gerald W Hoffmann, Y. Fisyak, Y. Wang, R. F. Clarke, B. Stringfellow, D. R. Gangadharan, E. G. Judd, Ki-Seok Choi, J. Sandweiss, H. Z. Huang, S. Vokal, M. DePhillips, J. Schambach, Michal Sumbera, W. Guryn, A. A. P. Suaide, Anju Bhasin, S. Bueltmann, O. G. Grebenyuk, R. P. Redwine, Y. Bai, S. W. Wissink, Y. Zhang, J. G. Cramer, Nikola Poljak, William Axel Leight, K. Kang, A. Shabetai, G. Wang, Elena Bruna, J. Baudot, J. Joseph, S. R. Klein, T. Tarnowsky, N. L. Subba, A. Lebedev, J. Kiryluk, J. H. Chen, G. M.S. Vasconcelos, M. Sarsour, D. A. Morozov, J. Fedorisin, M. Botje, Q. H. Xu, A. G. Knospe, Y. Pandit, D. L. Olson, Z. J. Sun, T. P. Burton, N. Schmitz, E. Shahaliev, H. Bichsel, G. Odyniec, A. A. Derevschikov, A. L. Trattner, Y. Zoulkarneeva, C. Zhong, Russell Richard Betts, P. Djawotho, D. G. Underwood, Jana Bielcikova, P. Sorensen, X. H. Shi, X. F. Wang, W. He, Peter Graham Jones, David Lynn, Q. Wang, M. J. Russcher, T. J. Hallman, J. Cheng, T. J. M. Symons, L. Didenko, Jing Zhou, M. Sharma, B. Erazmus, N. G. Minaev, N. Xu, P. Filip, Neeraj Gupta, D. Cebra, M. Estienne, G. D. Westfall, Petr Jákl, C. A. Whitten, Feng Jin, L. Ruan, R. Corliss, S. J. Lindenbaum, Basanta Kumar Nandi, B. Biritz, C. Roy, C. De Silva, Y. Xu, P. Chaloupka, Z. J. Xu, S. Trentalange, A. Chikanian, V. N. Tram, Ermes Braidot, D. Arkhipkin, Adam Ryszard Kisiel, H. J. Crawford, A. Hamed, R. Fatemi, Michael Joseph Betancourt, K. Krueger, A. Iordanova, J. A. Vanfossen, J. Callner, M. Cherney, Thomas Michael Cormier, Lokesh Kumar, B. Haag, Zebo Tang, Z. Chajecki, C. Perkins, T. Peitzmann, P. Kurnadi, M. R. Cosentino, F. Benedosso, T. G. Dedovich, T. Sakuma, S. C. Phatak, V. Fine, Jake Y. Chen, F. Du, V. Rykov, L. V. Nogach, M. J. Ng, V. I. Kravtsov, E. P. Sichtermann, L. C. Bland, J. Balewski, Rashmi Raniwala, Claude Andre Pruneau, L. Liu, M. C. Suarez, T. Ullrich, R. Zoulkarneev, L. Eun, M. S. Daugherity, R. L. Ray, D. Staszak, J. Ulery, H. G. Ritter, T. Ljubicic, O. Barannikova, R. Bellwied, L. Gaillard, H. Caines, Sudhir Raniwala, M. Wada, X. L. Wang, S. Margetis, M. van Leeuwen, X. Dong, and S. K. Pal
Subjects: Physics, Nuclear and High Energy Physics, Particle physics, Meson, 010308 nuclear & particles physics, High Energy Physics::Phenomenology, Nuclear Theory, Hadron, Strangeness, Lambda baryon, Phi meson, 01 natural sciences, Nuclear physics, Baryon, Particle decay, Pair production, 0103 physical sciences, High Energy Physics::Experiment, Nuclear Experiment, 010306 general physics
Abstract: We study the beam-energy and system-size dependence of phi meson production (using the hadronic decay mode phi -> K(+) K(-)) by comparing the new results from Cu + Cu collisions and previously reported Au + Au collisions at root s(NN) = 62.4 and 200 GeV measured in the STAR experiment at RHIC. Data presented in this Letter are from mid-rapidity (vertical bar y vertical bar ) and net strangeness = 2 (Xi). The enhancement for phi mesons is observed to be higher at root s(NN) = 200 GeV compared to 62.4 GeV. These observations for the produced phi(s (s) over bar) mesons clearly suggest that, at these collision energies, the source of enhancement of strange hadrons is related to the formation of a dense partonic medium in high energy nucleus-nucleus collisions and cannot be alone due to canonical suppression of their production in smaller systems. (C) 2009 Elsevier B.V. All rights reserved.
Published: 2009

39. Energy dependence of π±, p and p¯ transverse momentum spectra for Au+Au collisions at sNN=62.4 and 200 GeV

Author: B.I. Abelev, M.M. Aggarwal, Z. Ahammed, B.D. Anderson, D. Arkhipkin, G.S. Averichev, Y. Bai, J. Balewski, O. Barannikova, L.S. Barnby, S. Baumgart, V.V. Belaga, A. Bellingeri-Laurikainen, R. Bellwied, F. Benedosso, R.R. Betts, S. Bharadwaj, A. Bhasin, A.K. Bhati, H. Bichsel, J. Bielcik, J. Bielcikova, A. Billmeier, L.C. Bland, S.-L. Blyth, M. Bombara, B.E. Bonner, M. Botje, J. Bouchet, A.V. Brandin, A. Bravar, T.P. Burton, M. Bystersky, R.V. Cadman, X.Z. Cai, H. Caines, M. Calderón de la Barca Sánchez, J. Callner, O. Catu, D. Cebra, Z. Chajecki, P. Chaloupka, S. Chattopadhyay, H.F. Chen, J.H. Chen, J.Y. Chen, J. Cheng, M. Cherney, A. Chikanian, H.A. Choi, W. Christie, S.U. Chung, J.P. Coffin, T.M. Cormier, M.R. Cosentino, J.G. Cramer, H.J. Crawford, D. Das, S. Dash, M. Daugherity, M.M. de Moura, T.G. Dedovich, M. DePhillips, A.A. Derevschikov, L. Didenko, T. Dietel, P. Djawotho, S.M. Dogra, X. Dong, J.L. Drachenberg, J.E. Draper, F. Du, V.B. Dunin, J.C. Dunlop, M.R. Dutta Mazumdar, V. Eckardt, W.R. Edwards, L.G. Efimov, V. Emelianov, J. Engelage, G. Eppley, B. Erazmus, M. Estienne, P. Fachini, R. Fatemi, J. Fedorisin, A. Feng, P. Filip, E. Finch, V. Fine, Y. Fisyak, K.S.F. Fornazier, J. Fu, C.A. Gagliardi, L. Gaillard, M.S. Ganti, E. Garcia-Solis, V. Ghazikhanian, P. Ghosh, Y.G. Gorbunov, H. Gos, O. Grebenyuk, D. Grosnick, S.M. Guertin, K.S.F.F. Guimaraes, N. Gupta, B. Haag, T.J. Hallman, A. Hamed, J.W. Harris, W. He, M. Heinz, T.W. Henry, S. Hepplemann, B. Hippolyte, A. Hirsch, E. Hjort, A.M. Hoffman, G.W. Hoffmann, D. Hofman, R. Hollis, M.J. Horner, H.Z. Huang, E.W. Hughes, T.J. Humanic, G. Igo, A. Iordanova, P. Jacobs, W.W. Jacobs, P. Jakl, F. Jia, H. Jiang, P.G. Jones, E.G. Judd, S. Kabana, K. Kang, J. Kapitan, M. Kaplan, D. Keane, A. Kechechyan, D. Kettler, V.Yu. Khodyrev, B.C. Kim, J. Kiryluk, A. Kisiel, E.M. Kislov, S.R. Klein, A.G. Knospe, A. Kocoloski, D.D. Koetke, T. Kollegger, M. Kopytine, L. Kotchenda, V. Kouchpil, K.L. Kowalik, M. Kramer, P. Kravtsov, V.I. Kravtsov, K. Krueger, C. Kuhn, A.I. Kulikov, A. Kumar, P. Kurnadi, A.A. Kuznetsov, M.A.C. Lamont, J.M. Landgraf, S. Lange, S. LaPointe, F. Laue, J. Lauret, A. Lebedev, R. Lednicky, C.-H. Lee, S. Lehocka, M.J. LeVine, C. Li, Q. Li, Y. Li, G. Lin, X. Lin, S.J. Lindenbaum, M.A. Lisa, F. Liu, H. Liu, J. Liu, L. Liu, T. Ljubicic, W.J. Llope, H. Long, R.S. Longacre, M. Lopez-Noriega, W.A. Love, Y. Lu, T. Ludlam, D. Lynn, G.L. Ma, J.G. Ma, Y.G. Ma, D.P. Mahapatra, R. Majka, L.K. Mangotra, R. Manweiler, S. Margetis, C. Markert, L. Martin, H.S. Matis, Yu.A. Matulenko, C.J. McClain, T.S. McShane, Yu. Melnick, A. Meschanin, J. Millane, M.L. Miller, N.G. Minaev, S. Mioduszewski, C. Mironov, A. Mischke, J. Mitchell, B. Mohanty, L. Molnar, D.A. Morozov, M.G. Munhoz, B.K. Nandi, C. Nattrass, T.K. Nayak, J.M. Nelson, C. Nepali, P.K. Netrakanti, V.A. Nikitin, L.V. Nogach, S.B. Nurushev, G. Odyniec, A. Ogawa, V. Okorokov, M. Oldenburg, D. Olson, M. Pachr, S.K. Pal, Y. Panebratsev, S.Y. Panitkin, A.I. Pavlinov, T. Pawlak, T. Peitzmann, V. Perevoztchikov, C. Perkins, W. Peryt, S.C. Phatak, M. Planinic, J. Pluta, N. Poljak, N. Porile, A.M. Poskanzer, M. Potekhin, E. Potrebenikova, B.V.K.S. Potukuchi, D. Prindle, C. Pruneau, J. Putschke, I.A. Qattan, R. Raniwala, S. Raniwala, R.L. Ray, S.V. Razin, J. Reinnarth, D. Relyea, A. Ridiger, H.G. Ritter, J.B. Roberts, O.V. Rogachevskiy, J.L. Romero, A. Rose, C. Roy, L. Ruan, M.J. Russcher, R. Sahoo, I. Sakrejda, T. Sakuma, S. Salur, J. Sandweiss, M. Sarsour, I. Savin, P.S. Sazhin, J. Schambach, R.P. Scharenberg, N. Schmitz, J. Seger, I. Selyuzhenkov, P. Seyboth, A. Shabetai, E. Shahaliev, M. Shao, M. Sharma, W.Q. Shen, S.S. Shimanskiy, E. Sichtermann, F. Simon, R.N. Singaraju, N. Smirnov, R. Snellings, P. Sorensen, J. Sowinski, J. Speltz, H.M. Spinka, B. Srivastava, A. Stadnik, T.D.S. Stanislaus, R. Stock, M. Strikhanov, B. Stringfellow, A.A.P. Suaide, M.C. Suarez, N.L. Subba, M. Sumbera, X.M. Sun, Z. Sun, B. Surrow, T.J.M. Symons, A. Szanto de Toledo, J. Takahashi, A.H. Tang, T. Tarnowsky, J.H. Thomas, A.R. Timmins, S. Timoshenko, M. Tokarev, T.A. Trainor, S. Trentalange, R.E. Tribble, O.D. Tsai, J. Ulery, T. Ullrich, D.G. Underwood, G. Van Buren, N. van der Kolk, M. van Leeuwen, A.M. Vander Molen, R. Varma, I.M. Vasilevski, A.N. Vasiliev, R. Vernet, S.E. Vigdor, Y.P. Viyogi, S. Vokal, S.A. Voloshin, W.T. Waggoner, F. Wang, G. Wang, J.S. Wang, X.L. Wang, Y. Wang, J.W. Watson, J.C. Webb, G.D. Westfall, A. Wetzler, C. Whitten Jr., H. Wieman, S.W. Wissink, R. Witt, J. Wu, N. Xu, Q.H. Xu, Z. Xu, P. Yepes, I.-K. Yoo, Q. Yue, V.I. Yurevich, W. Zhan, H. Zhang, W.M. Zhang, Y. Zhang, Z.P. Zhang, Y. Zhao, C. Zhong, R. Zoulkarneev, Y. Zoulkarneeva, A.N. Zubarev, and J.X. Zuo
Subjects: Nuclear reaction, Physics, Nuclear and High Energy Physics, Meson, Proton, 010308 nuclear & particles physics, Hadron, 01 natural sciences, Nuclear physics, Baryon, Pion, Antimatter, 0103 physical sciences, High Energy Physics::Experiment, Atomic physics, Nuclear Experiment, 010306 general physics, Jet quenching
Abstract: We study the energy dependense of the transverse momentum (PT) spectra for charged pions, protons and anti-protons for An + An collisions at root(NN)-N-s = 62.4 and 200 GeV. Data are presented at mid-rapidity (broken vertical bar y broken vertical bar 7 GeV/c) the modification is similar for both energies. The p/pi(+) and (p) over bar/pi(-) ratios for central collisions at root(NN)-N-s = 62.4 GeV peak at PT similar or equal to 2 GeV/c. In the PT range where recombination is expected to dominate, the p/pi(+) ratios at 62.4 GeV are larger than at 200 GeV, while the (p) over bar/pi(-) ratios are smaller. For PT > 2 GeV/c, the (p) over bar/pi(-) ratios at the two beam energies are independent of P-T and centrality indicating that the dependence of the (p) over bar/pi(-) ratio on P-T does not change between 62.4 and 200 GeV. These findings challenge various models incorporating jet quenching and/or constituent quark coalescence. (C) 2007 Elsevier B.V. All rights reserved.
Published: 2007

40. DLC solid lubricant coatings on ball bearings for space applications

Author: L. Eersels, F. Velasco, Danny Havermans, A. Vanhulsel, E. W. Roberts, Ian Sherrington, R. Jacobs, M. J. Anderson, and L. Gaillard
Subjects: Bearing (mechanical), Materials science, Hydrogen, Mechanical Engineering, chemistry.chemical_element, Surfaces and Interfaces, engineering.material, Tribology, Nitrogen, Surfaces, Coatings and Films, law.invention, Air bearing, Coating, chemistry, Mechanics of Materials, law, engineering, Lubrication, Lubricant, Composite material
Abstract: The environment of space offers special challenges for the lubrication of components in sliding and rolling mechanisms. Hydrogenated diamond-like carbon (DLC) films are being studied as solid lubricant coatings to simultaneously fulfil specifications regarding wear resistance and low friction behaviour under ambient atmosphere and in vacuum. In this paper, the tribological behaviour of highly hydrogenated DLC coatings ( ∼ 50 at% hydrogen) is assessed. Coating composition was optimised on flat AISI 52100 steel substrates based on ball-on-disc tribotest results in air, vacuum and dry nitrogen environments. The developed DLC coatings can be tailored to yield ultra-low friction values in vacuum ( μ = 0.008 ) . The average friction coefficient range obtained in humid air, dry nitrogen and vacuum for the range of applied loads were, respectively, 0.22 to 0.27, 0.02 to 0.03, and 0.007 to 0.013. New in this work is that optimised DLC coatings were applied to ball bearings for space applications. The torque and life tests of coated pairs of angular contact bearings in air revealed that relatively high bearing torques are generated which increase with time, but the amount of coating wear generated during in-air operation appears relatively light. In vacuum, low torques are generated after a prolonged running-in period. Low-torque life exceeds that observed for MoS 2 by a factor of about two. It is concluded that, in contrast to MoS 2 coated bearings, DLC-coated bearings for space applications might therefore be capable of undergoing in-air ground testing without too much disruption of the subsequent in-space performance.
Published: 2007

41. Trace Proteinuria

Author: Y. Manuel, J. P. Revillard, R. Fran�ois, J. Traeger, L. Gaillard, B. Salle, M. T. Freycon, and I. Borenstain
Published: 2015

42. Proteolysis of milk proteins by AprX, an extracellular protease identified in [i]Pseudomonas[/i] LBSA1 isolated from bulk raw milk, and implications for the stability of UHT milk

Author: M. Guyard-Nicodème, F. Baglinière, Julien Jardin, Annie Dary, Gérard Humbert, J.-L. Gaillard, Frédéric Gaucheron, Aurélie Matéos, Unité de Recherches Animal et Fonctionnalités des Produits Animaux (URAFPA), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), Aliments Bioprocédés Toxicologie Environnements (ABTE), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), and PhD and postdoctoral scholarships from CNIEL (National Interprofessional Center of the Dairy Economy).
Subjects: medicine.medical_treatment, Proteolysis, micelle de caséine, Applied Microbiology and Biotechnology, produit laitier, chemistry.chemical_compound, Hydrolysis, contamination, pseudomonas LBSA1, [SDV.IDA]Life Sciences [q-bio]/Food engineering, medicine, Aromatic amino acids, Proline, lait UHT, Protease, medicine.diagnostic_test, biology, Chemistry, Pseudomonas, conservation, Raw milk, biology.organism_classification, Serralysin, Biochemistry, peptide du lait, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Food Science, traitement thermique
Abstract: UHT milk made from milk contaminated by Pseudomonas LBSA1 destabilised during storage. Sedimentation of UHT milk was observed; zeta potential of casein micelles decreased, while contents of non-casein nitrogen and non-protein nitrogen increased. Pseudomonas LBSA1 produced an extracellular protease that hydrolysed caseins but not whey proteins; this was identified as AprX, a thermoresistant protease belonging to the serralysin family. This protease showed a broad range of pH activity (pH 6 to pH 10) and an optimal temperature of activity of 40 °C. Peptides released from purified αS1-, β- and κ-caseins were determined by tandem mass spectrometry. The identified cleavage sites did not reveal a strong specificity of the extracellular protease. However, the presence of basic or aromatic amino acid residues in the P1 position had a positive influence on cleavage in comparison with acidic amino acid residues or proline.
Published: 2015

43. The SLX4 Complex Is a SUMO E3 Ligase that Impacts on Replication Stress Outcome and Genome Stability

Author: Charly Chawhan, Emmanuelle Despras, Yoann Lovera, Filippo Rosselli, Arato Takedachi, Pierre-Henri L. Gaillard, Patricia Kannouche, Sarah Scaglione, Jean-Hugues Guervilly, Isao Kuraoka, Valeria Naim, gaillard, pierre-henri, Centre de Recherche en Cancérologie de Marseille (CRCM), Aix Marseille Université (AMU)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Stabilité Génétique et Oncogenèse (UMR 8200), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Centre National de la Recherche Scientifique (CNRS), Centre for Genome Damage and Stability, University of Sussex, Osaka University [Osaka], Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC), Aix Marseille Université (AMU), Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Paoli-Calmettes, Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Fédération nationale des Centres de lutte contre le Cancer (FNCLCC)-Aix Marseille Université (AMU), Graduate School of Frontier Biosciences, and Osaka University [Osaka]-Japan Science and Technology Agency-Core Research for Evolutional Science and Technology
Subjects: Genetics, biology, DNA repair, Chromosomal fragile site, Protein subunit, Regulator, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular Networks [q-bio.MN], [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC], Cell Biology, Ubiquitin ligase, Endonuclease, chemistry.chemical_compound, chemistry, [SDV.GEN.GH]Life Sciences [q-bio]/Genetics/Human genetics, biology.protein, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Mitotic catastrophe, Molecular Biology, DNA, ComputingMilieux_MISCELLANEOUS
Abstract: International audience; The SLX4 Fanconi anemia protein is a tumor suppres- sor that may act as a key regulator that engages the cell into specific genome maintenance pathways. Here, we show that the SLX4 complex is a SUMO E3 ligase that SUMOylates SLX4 itself and the XPF sub- unit of the DNA repair/recombination XPF-ERCC1 endonuclease. This SLX4-dependent activity is medi- ated by a remarkably specific interaction between SLX4 and the SUMO-charged E2 conjugating enzyme UBC9 and relies not only on newly identified SUMO- interacting motifs (SIMs) in SLX4 but also on its BTB domain. In contrast to its ubiquitin-binding UBZ4 motifs, SLX4 SIMs are dispensable for its DNA inter- strand crosslink repair functions. Instead, while detri- mental in response to global replication stress, the SUMO E3 ligase activity of the SLX4 complex is crit- ical to prevent mitotic catastrophe following common fragile site expression.
Published: 2015

44. Identified two-hadron correlations at STAR using Λ, Λ̄, and K0 Short triggers with charged hadrons in Au + Au collisions at $\sqrt{s_{NN}}$ =200 GeV

Author: L. Gaillard
Subjects: Physics, Particle physics, Physics and Astronomy (miscellaneous), Astrophysics::High Energy Astrophysical Phenomena, Hadron, Elementary particle, Jet (particle physics), Star (graph theory), Ridge (differential geometry), Nuclear physics, Angular correlation, Correlation analysis, Nuclear Experiment, Engineering (miscellaneous)
Abstract: Here we present initial studies of two-dimensional angular correlations of Λ, Λ, and K0Short triggers with unidentified charged hadrons in Au+Au collisions at $\sqrt{s_{NN}}$ =200 GeV measured by STAR. Distributions of pseudo-rapidity difference, Δη, and azimuthal separation, Δφ, are constructed containing structures observed in unidentified hadron correlations, including a jet peak at small Δη-Δφ accompanied by a flow-like ridge extended over Δη. These features are studied as a function of centrality via integrated yields and fitting to projections onto Δη and Δφ axes. Yields are found to be consistent with unidentified correlation analyses, and no clear distinction is observed between the three species.
Published: 2006

45. Transverse-momentumptcorrelations on (η, phi) from mean-ptfluctuations in Au–Au collisions at GeV

Author: STAR Collaboration, J Adams, M M Aggarwal, Z Ahammed, J Amonett, B D Anderson, D Arkhipkin, G S Averichev, S K Badyal, Y Bai, J Balewski, O Barannikova, L S Barnby, J Baudot, S Bekele, V V Belaga, A Bellingeri-Laurikainen, R Bellwied, J Berger, B I Bezverkhny, S Bharadwaj, A Bhasin, A K Bhati, V S Bhatia, H Bichsel, J Bielcik, J Bielcikova, A Billmeier, L C Bland, C O Blyth, S-L Blyth, B E Bonner, M Botje, A Boucham, J Bouchet, A V Brandin, A Bravar, M Bystersky, R V Cadman, X Z Cai, H Caines, M Calderón de la Barca Sánchez, J Castillo, O Catu, D Cebra, Z Chajecki, P Chaloupka, S Chattopadhyay, H F Chen, J H Chen, Y Chen, J Cheng, M Cherney, A Chikanian, H A Choi, W Christie, J P Coffin, T M Cormier, M R Cosentino, J G Cramer, H J Crawford, D Das, S Das, M Daugherity, M M de Moura, T G Dedovich, M DePhillips, A A Derevschikov, L Didenko, T Dietel, S M Dogra, W J Dong, X Dong, J E Draper, F Du, A K Dubey, V B Dunin, J C Dunlop, M R Dutta Mazumdar, V Eckardt, W R Edwards, L G Efimov, V Emelianov, J Engelage, G Eppley, B Erazmus, M Estienne, P Fachini, J Faivre, R Fatemi, J Fedorisin, K Filimonov, P Filip, E Finch, V Fine, Y Fisyak, K S F Fornazier, J Fu, C A Gagliardi, L Gaillard, J Gans, M S Ganti, F Geurts, V Ghazikhanian, P Ghosh, J E Gonzalez, H Gos, O Grachov, O Grebenyuk, D Grosnick, S M Guertin, Y Guo, A Gupta, N Gupta, T D Gutierrez, T J Hallman, A Hamed, D Hardtke, J W Harris, M Heinz, T W Henry, S Hepplemann, B Hippolyte, A Hirsch, E Hjort, G W Hoffmann, M J Horner, H Z Huang, S L Huang, E W Hughes, T J Humanic, G Igo, A Ishihara, P Jacobs, W W Jacobs, M Jedynak, H Jiang, P G Jones, E G Judd, S Kabana, K Kang, M Kaplan, D Keane, A Kechechyan, V Yu Khodyrev, B C Kim, J Kiryluk, A Kisiel, E M Kislov, J Klay, S R Klein, D D Koetke, T Kollegger, M Kopytine, L Kotchenda, K L Kowalik, M Kramer, P Kravtsov, V I Kravtsov, K Krueger, C Kuhn, A I Kulikov, A Kumar, R Kh Kutuev, A A Kuznetsov, M A C Lamont, J M Landgraf, S Lange, F Laue, J Lauret, A Lebedev, R Lednicky, C-H Lee, S Lehocka, M J LeVine, C Li, Q Li, Y Li, G Lin, S J Lindenbaum, M A Lisa, F Liu, H Liu, J Liu, L Liu, Q J Liu, Z Liu, T Ljubicic, W J Llope, H Long, R S Longacre, M Lopez-Noriega, W A Love, Y Lu, T Ludlam, D Lynn, G L Ma, J G Ma, Y G Ma, D Magestro, S Mahajan, D P Mahapatra, R Majka, L K Mangotra, R Manweiler, S Margetis, C Markert, L Martin, J N Marx, H S Matis, Yu A Matulenko, C J McClain, T S McShane, F Meissner, Yu Melnick, A Meschanin, M L Miller, N G Minaev, C Mironov, A Mischke, D K Mishra, J Mitchell, B Mohanty, L Molnar, C F Moore, D A Morozov, M G Munhoz, B K Nandi, S K Nayak, T K Nayak, J M Nelson, P K Netrakanti, V A Nikitin, L V Nogach, S B Nurushev, G Odyniec, A Ogawa, V Okorokov, M Oldenburg, D Olson, S K Pal, Y Panebratsev, S Y Panitkin, A I Pavlinov, T Pawlak, T Peitzmann, V Perevoztchikov, C Perkins, W Peryt, V A Petrov, S C Phatak, R Picha, M Planinic, J Pluta, and N Porile
Subjects: Physics, Nuclear and High Energy Physics, Particle physics, 010308 nuclear & particles physics, Pseudorapidity, 0103 physical sciences, Transverse momentum, Dissipative system, High Energy Physics::Experiment, Parton, Nuclear Experiment, 010306 general physics, 01 natural sciences
Abstract: We present first measurements of the pseudorapidity and azimuth $(\eta,\phi)$ bin-size dependence of event-wise mean transverse momentum $ $ fluctuations for Au-Au collisions at $\sqrt{s_{NN}} = 200$ GeV. We invert that dependence to obtain $p_t$ autocorrelations on differences $(\eta_\Delta,\phi_\Delta)$ interpreted to represent velocity/temperature distributions on ($\eta,\phi$). The general form of the autocorrelations suggests that the basic correlation mechanism is parton fragmentation. The autocorrelations vary strongly with collision centrality, which suggests that fragmentation is strongly modified by a dissipative medium in the more central Au-Au collisions relative to peripheral or p-p collisions. \\
Published: 2006

46. Hadronization geometry from net-charge angular correlations on momentum subspace (η,ϕ) in Au–Au collisions at sNN=130 GeV

Author: J. Adams, M.M. Aggarwal, Z. Ahammed, J. Amonett, B.D. Anderson, D. Arkhipkin, G.S. Averichev, S.K. Badyal, Y. Bai, J. Balewski, O. Barannikova, L.S. Barnby, J. Baudot, S. Bekele, V.V. Belaga, A. Bellingeri-Laurikainen, R. Bellwied, J. Berger, B.I. Bezverkhny, S. Bharadwaj, A. Bhasin, A.K. Bhati, V.S. Bhatia, H. Bichsel, J. Bielcik, J. Bielcikova, A. Billmeier, L.C. Bland, C.O. Blyth, S.-L. Blyth, B.E. Bonner, M. Botje, A. Boucham, J. Bouchet, A.V. Brandin, A. Bravar, M. Bystersky, R.V. Cadman, X.Z. Cai, H. Caines, M. Calderón de la Barca Sánchez, J. Castillo, O. Catu, D. Cebra, Z. Chajecki, P. Chaloupka, S. Chattopadhyay, H.F. Chen, J.H. Chen, Y. Chen, J. Cheng, M. Cherney, A. Chikanian, H.A. Choi, W. Christie, J.P. Coffin, T.M. Cormier, M.R. Cosentino, J.G. Cramer, H.J. Crawford, D. Das, S. Das, M. Daugherity, M.M. de Moura, T.G. Dedovich, M. DePhillips, A.A. Derevschikov, L. Didenko, T. Dietel, S.M. Dogra, W.J. Dong, X. Dong, J.E. Draper, F. Du, A.K. Dubey, V.B. Dunin, J.C. Dunlop, M.R. Dutta Mazumdar, V. Eckardt, W.R. Edwards, L.G. Efimov, V. Emelianov, J. Engelage, G. Eppley, B. Erazmus, M. Estienne, P. Fachini, J. Faivre, R. Fatemi, J. Fedorisin, K. Filimonov, P. Filip, E. Finch, V. Fine, Y. Fisyak, K.S.F. Fornazier, J. Fu, C.A. Gagliardi, L. Gaillard, J. Gans, M.S. Ganti, F. Geurts, V. Ghazikhanian, P. Ghosh, J.E. Gonzalez, H. Gos, O. Grachov, O. Grebenyuk, D. Grosnick, S.M. Guertin, Y. Guo, A. Gupta, N. Gupta, T.D. Gutierrez, T.J. Hallman, A. Hamed, D. Hardtke, J.W. Harris, M. Heinz, T.W. Henry, S. Hepplemann, B. Hippolyte, A. Hirsch, E. Hjort, G.W. Hoffmann, M.J. Horner, H.Z. Huang, S.L. Huang, E.W. Hughes, T.J. Humanic, G. Igo, A. Ishihara, P. Jacobs, W.W. Jacobs, M. Jedynak, H. Jiang, P.G. Jones, E.G. Judd, S. Kabana, K. Kang, M. Kaplan, D. Keane, A. Kechechyan, V.Yu. Khodyrev, B.C. Kim, J. Kiryluk, A. Kisiel, E.M. Kislov, J. Klay, S.R. Klein, D.D. Koetke, T. Kollegger, M. Kopytine, L. Kotchenda, K.L. Kowalik, M. Kramer, P. Kravtsov, V.I. Kravtsov, K. Krueger, C. Kuhn, A.I. Kulikov, A. Kumar, R.Kh. Kutuev, A.A. Kuznetsov, M.A.C. Lamont, J.M. Landgraf, S. Lange, F. Laue, J. Lauret, A. Lebedev, R. Lednicky, C.-H. Lee, S. Lehocka, M.J. LeVine, C. Li, Q. Li, Y. Li, G. Lin, S.J. Lindenbaum, M.A. Lisa, F. Liu, H. Liu, J. Liu, L. Liu, Q.J. Liu, Z. Liu, T. Ljubicic, W.J. Llope, H. Long, R.S. Longacre, M. Lopez-Noriega, W.A. Love, Y. Lu, T. Ludlam, D. Lynn, G.L. Ma, J.G. Ma, Y.G. Ma, D. Magestro, S. Mahajan, D.P. Mahapatra, R. Majka, L.K. Mangotra, R. Manweiler, S. Margetis, C. Markert, L. Martin, J.N. Marx, H.S. Matis, Yu.A. Matulenko, C.J. McClain, T.S. McShane, F. Meissner, Yu. Melnick, A. Meschanin, M.L. Miller, N.G. Minaev, C. Mironov, A. Mischke, D.K. Mishra, J. Mitchell, B. Mohanty, L. Molnar, C.F. Moore, D.A. Morozov, M.G. Munhoz, B.K. Nandi, S.K. Nayak, T.K. Nayak, J.M. Nelson, P.K. Netrakanti, V.A. Nikitin, L.V. Nogach, S.B. Nurushev, G. Odyniec, A. Ogawa, V. Okorokov, M. Oldenburg, D. Olson, S.K. Pal, Y. Panebratsev, S.Y. Panitkin, A.I. Pavlinov, T. Pawlak, T. Peitzmann, V. Perevoztchikov, C. Perkins, W. Peryt, V.A. Petrov, S.C. Phatak, R. Picha, M. Planinic, J. Pluta, N. Porile, J. Porter, A.M. Poskanzer, M. Potekhin, E. Potrebenikova, B.V.K.S. Potukuchi, D. Prindle, C. Pruneau, J. Putschke, G. Rakness, R. Raniwala, S. Raniwala, O. Ravel, R.L. Ray, S.V. Razin, D. Reichhold, J.G. Reid, J. Reinnarth, G. Renault, F. Retiere, A. Ridiger, H.G. Ritter, J.B. Roberts, O.V. Rogachevskiy, J.L. Romero, A. Rose, C. Roy, L. Ruan, M.J. Russcher, R. Sahoo, I. Sakrejda, S. Salur, J. Sandweiss, M. Sarsour, I. Savin, P.S. Sazhin, J. Schambach, R.P. Scharenberg, N. Schmitz, K. Schweda, J. Seger, P. Seyboth, E. Shahaliev, M. Shao, W. Shao, M. Sharma, W.Q. Shen, K.E. Shestermanov, S.S. Shimanskiy, E. Sichtermann, F. Simon, R.N. Singaraju, N. Smirnov, R. Snellings, G. Sood, P. Sorensen, J. Sowinski, J. Speltz, H.M. Spinka, B. Srivastava, A. Stadnik, T.D.S. Stanislaus, R. Stock, A. Stolpovsky, M. Strikhanov, B. Stringfellow, A.A.P. Suaide, E. Sugarbaker, M. Sumbera, B. Surrow, M. Swanger, T.J.M. Symons, A. Szanto de Toledo, A. Tai, J. Takahashi, A.H. Tang, T. Tarnowsky, D. Thein, J.H. Thomas, A.R. Timmins, S. Timoshenko, M. Tokarev, T.A. Trainor, S. Trentalange, R.E. Tribble, O.D. Tsai, J. Ulery, T. Ullrich, D.G. Underwood, G. Van Buren, N. van der Kolk, M. van Leeuwen, A.M. Vander Molen, R. Varma, I.M. Vasilevski, A.N. Vasiliev, R. Vernet, S.E. Vigdor, Y.P. Viyogi, S. Vokal, S.A. Voloshin, W.T. Waggoner, F. Wang, G. Wang, X.L. Wang, Y. Wang, Z.M. Wang, H. Ward, J.W. Watson, J.C. Webb, G.D. Westfall, A. Wetzler, C. Whitten, H. Wieman, S.W. Wissink, R. Witt, J. Wood, J. Wu, N. Xu, Z. Xu, Z.Z. Xu, E. Yamamoto, P. Yepes, I.-K. Yoo, V.I. Yurevich, I. Zborovsky, H. Zhang, W.M. Zhang, Y. Zhang, Z.P. Zhang, C. Zhong, R. Zoulkarneev, Y. Zoulkarneeva, A.N. Zubarev, and J.X. Zuo
Subjects: Physics, Nuclear and High Energy Physics, Particle physics, 010308 nuclear & particles physics, Hadron, Angular difference, Geometry, 01 natural sciences, Hadronization, Azimuth, Nuclear physics, Pseudorapidity, 0103 physical sciences, Transverse momentum, Beam direction, High Energy Physics::Experiment, Nuclear Experiment, 010306 general physics, Subspace topology
Abstract: We present the first measurements of charge-dependent correlations on angular difference variables η 1 − η 2 (pseudorapidity) and ϕ 1 − ϕ 2 (azimuth) for primary charged hadrons with transverse momentum 0.15 ⩽ p t ⩽ 2 GeV / c and | η | ⩽ 1.3 from Au–Au collisions at s N N = 130 GeV . We observe correlation structures not predicted by theory but consistent with evolution of hadron emission geometry with increasing centrality from one-dimensional fragmentation of color strings along the beam direction to an at least two-dimensional hadronization geometry along the beam and azimuth directions of a hadron-opaque bulk medium.
Published: 2006

47. Friction and Wear of Self-Lubricating Polyimides in Vacuum and Air to High Temperatures

Author: A. Merstallinger, H. Bieringer, L. Gaillard, Josef Brenner, and E. Kubinger
Subjects: Materials science, Mechanical Engineering, General Chemical Engineering, Surfaces, Coatings and Films
Published: 2005

48. Pion, kaon, proton and anti-proton transverse momentum distributions from p+p and d+Au collisions at sNN=200 GeV

Author: J. Adams, M.M. Aggarwal, Z. Ahammed, J. Amonett, B.D. Anderson, D. Arkhipkin, G.S. Averichev, S.K. Badyal, Y. Bai, J. Balewski, O. Barannikova, L.S. Barnby, J. Baudot, S. Bekele, V.V. Belaga, R. Bellwied, J. Berger, B.I. Bezverkhny, S. Bharadwaj, A. Bhasin, A.K. Bhati, V.S. Bhatia, H. Bichsel, J. Bielcik, J. Bielcikova, A. Billmeier, L.C. Bland, C.O. Blyth, B.E. Bonner, M. Botje, A. Boucham, A.V. Brandin, A. Bravar, M. Bystersky, R.V. Cadman, X.Z. Cai, H. Caines, M. Calderón de la Barca Sánchez, J. Castillo, O. Catu, D. Cebra, Z. Chajecki, P. Chaloupka, S. Chattopadhyay, H.F. Chen, Y. Chen, J. Cheng, M. Cherney, A. Chikanian, W. Christie, J.P. Coffin, T.M. Cormier, J.G. Cramer, H.J. Crawford, D. Das, S. Das, M.M. de Moura, A.A. Derevschikov, L. Didenko, T. Dietel, S.M. Dogra, W.J. Dong, X. Dong, J.E. Draper, F. Du, A.K. Dubey, V.B. Dunin, J.C. Dunlop, M.R. Dutta Mazumdar, V. Eckardt, W.R. Edwards, L.G. Efimov, V. Emelianov, J. Engelage, G. Eppley, B. Erazmus, M. Estienne, P. Fachini, J. Faivre, R. Fatemi, J. Fedorisin, K. Filimonov, P. Filip, E. Finch, V. Fine, Y. Fisyak, K. Fomenko, J. Fu, C.A. Gagliardi, L. Gaillard, J. Gans, M.S. Ganti, L. Gaudichet, F. Geurts, V. Ghazikhanian, P. Ghosh, J.E. Gonzalez, O. Grachov, O. Grebenyuk, D. Grosnick, S.M. Guertin, Y. Guo, A. Gupta, T.D. Gutierrez, T.J. Hallman, A. Hamed, D. Hardtke, J.W. Harris, M. Heinz, T.W. Henry, S. Hepplemann, B. Hippolyte, A. Hirsch, E. Hjort, G.W. Hoffmann, H.Z. Huang, S.L. Huang, E.W. Hughes, T.J. Humanic, G. Igo, A. Ishihara, P. Jacobs, W.W. Jacobs, M. Janik, H. Jiang, P.G. Jones, E.G. Judd, S. Kabana, K. Kang, M. Kaplan, D. Keane, V.Yu. Khodyrev, J. Kiryluk, A. Kisiel, E.M. Kislov, J. Klay, S.R. Klein, D.D. Koetke, T. Kollegger, M. Kopytine, L. Kotchenda, M. Kramer, P. Kravtsov, V.I. Kravtsov, K. Krueger, C. Kuhn, A.I. Kulikov, A. Kumar, R.Kh. Kutuev, A.A. Kuznetsov, M.A.C. Lamont, J.M. Landgraf, S. Lange, F. Laue, J. Lauret, A. Lebedev, R. Lednicky, S. Lehocka, M.J. LeVine, C. Li, Q. Li, Y. Li, G. Lin, S.J. Lindenbaum, M.A. Lisa, F. Liu, L. Liu, Q.J. Liu, Z. Liu, T. Ljubicic, W.J. Llope, H. Long, R.S. Longacre, M. Lopez-Noriega, W.A. Love, Y. Lu, T. Ludlam, D. Lynn, G.L. Ma, J.G. Ma, Y.G. Ma, D. Magestro, S. Mahajan, D.P. Mahapatra, R. Majka, L.K. Mangotra, R. Manweiler, S. Margetis, C. Markert, L. Martin, J.N. Marx, H.S. Matis, Yu.A. Matulenko, C.J. McClain, T.S. McShane, F. Meissner, Yu. Melnick, A. Meschanin, M.L. Miller, N.G. Minaev, C. Mironov, A. Mischke, D.K. Mishra, J. Mitchell, B. Mohanty, L. Molnar, C.F. Moore, D.A. Morozov, M.G. Munhoz, B.K. Nandi, S.K. Nayak, T.K. Nayak, J.M. Nelson, P.K. Netrakanti, V.A. Nikitin, L.V. Nogach, S.B. Nurushev, G. Odyniec, A. Ogawa, V. Okorokov, M. Oldenburg, D. Olson, S.K. Pal, Y. Panebratsev, S.Y. Panitkin, A.I. Pavlinov, T. Pawlak, T. Peitzmann, V. Perevoztchikov, C. Perkins, W. Peryt, V.A. Petrov, S.C. Phatak, R. Picha, M. Planinic, J. Pluta, N. Porile, J. Porter, A.M. Poskanzer, M. Potekhin, E. Potrebenikova, B.V.K.S. Potukuchi, D. Prindle, C. Pruneau, J. Putschke, G. Rakness, R. Raniwala, S. Raniwala, O. Ravel, R.L. Ray, S.V. Razin, D. Reichhold, J.G. Reid, G. Renault, F. Retiere, A. Ridiger, H.G. Ritter, J.B. Roberts, O.V. Rogachevskiy, J.L. Romero, A. Rose, C. Roy, L. Ruan, R. Sahoo, I. Sakrejda, S. Salur, J. Sandweiss, M. Sarsour, I. Savin, P.S. Sazhin, J. Schambach, R.P. Scharenberg, N. Schmitz, K. Schweda, J. Seger, P. Seyboth, E. Shahaliev, M. Shao, W. Shao, M. Sharma, W.Q. Shen, K.E. Shestermanov, S.S. Shimanskiy, E. Sichtermann, F. Simon, R.N. Singaraju, G. Skoro, N. Smirnov, R. Snellings, G. Sood, P. Sorensen, J. Sowinski, J. Speltz, H.M. Spinka, B. Srivastava, A. Stadnik, T.D.S. Stanislaus, R. Stock, A. Stolpovsky, M. Strikhanov, B. Stringfellow, A.A.P. Suaide, E. Sugarbaker, C. Suire, M. Sumbera, B. Surrow, T.J.M. Symons, A. Szanto de Toledo, P. Szarwas, A. Tai, J. Takahashi, A.H. Tang, T. Tarnowsky, D. Thein, J.H. Thomas, S. Timoshenko, M. Tokarev, T.A. Trainor, S. Trentalange, R.E. Tribble, O.D. Tsai, J. Ulery, T. Ullrich, D.G. Underwood, A. Urkinbaev, G. Van Buren, M. van Leeuwen, A.M. Vander Molen, R. Varma, I.M. Vasilevski, A.N. Vasiliev, R. Vernet, S.E. Vigdor, Y.P. Viyogi, S. Vokal, S.A. Voloshin, M. Vznuzdaev, W.T. Waggoner, F. Wang, G. Wang, X.L. Wang, Y. Wang, Z.M. Wang, H. Ward, J.W. Watson, J.C. Webb, R. Wells, G.D. Westfall, A. Wetzler, C. Whitten, H. Wieman, S.W. Wissink, R. Witt, J. Wood, J. Wu, N. Xu, Z. Xu, Z.Z. Xu, E. Yamamoto, P. Yepes, V.I. Yurevich, Y.V. Zanevsky, H. Zhang, W.M. Zhang, Z.P. Zhang, R. Zoulkarneev, Y. Zoulkarneeva, and A.N. Zubarev
Subjects: Quantum chromodynamics, Physics, Nuclear and High Energy Physics, Proton, 010308 nuclear & particles physics, Hadron, 7. Clean energy, 01 natural sciences, Particle identification, Baryon, Nuclear physics, Pion, Antiproton, 0103 physical sciences, High Energy Physics::Experiment, Nuclear Experiment, 010306 general physics, Bar (unit)
Abstract: Identified mid-rapidity particle spectra of pi(+/-), K+/-, and p((p) over bar) from 200 GeV p + p and d + Au collisions are reported. A time-of-flight detector based on multi-gap resistive plate chamber technology is used for particle identification. The particle-species dependence of the Cronin effect is observed to be significantly smaller than that at lower energies. The ratio of the nuclear modification factor (R-dAu) between protons (p + (p) over bar) and charged hadrons (h) in the transverse momentum range 1.2 < p(T) < 3.0 GeV/c is measured to be 1.19 +/- 0.05(stat) +/- 0.03(syst) in minimum-bias collisions and shows little centrality dependence. The yield ratio of (p + (p) over bar)/h in minimum-bias d + Au collisions is found to be a factor of 2 lower than that in Au + Au collisions, indicating that the Cronin effect alone is not enough to account for the relative baryon enhancement observed in heavy ion collisions at RHIC. (c) 2005 Elsevier B.V. All rights reserved.
Published: 2005

49. RNA Interference Inhibition of Mus81 Reduces Mitotic Recombination in Human Cells

Author: Veronique Blais, Michael N. Boddy, Paul Russell, Cherilyn A. Elwell, Clare H. McGowan, Hui Gao, and Pierre-Henri L. Gaillard
Subjects: Saccharomyces cerevisiae Proteins, Mitotic crossover, Cell Survival, Nucleolus, Protein subunit, Molecular Sequence Data, Mitosis, DNA-binding protein, Endonuclease, RNA interference, Humans, Amino Acid Sequence, Cloning, Molecular, Molecular Biology, Recombination, Genetic, biology, Holliday Junction Resolvases, Articles, Cell Biology, Endonucleases, MUS81, Molecular biology, DNA-Binding Proteins, biology.protein, RNA Interference, Schizosaccharomyces pombe Proteins, Cell Nucleolus, DNA Damage, HeLa Cells, Protein Binding
Abstract: Mus81 is a highly conserved endonuclease with homology to the XPF subunit of the XPF-ERCC1 complex. In yeast Mus81 associates with a second subunit, Eme1 or Mms4, which is essential for endonuclease activity in vitro and for in vivo function. Human Mus81 binds to a homolog of fission yeast Eme1 in vitro and in vivo. We show that recombinant Mus81-Eme1 cleaves replication forks, 3′ flap substrates, and Holliday junctions in vitro. By use of differentially tagged versions of Mus81 and Eme1, we find that Mus81 associates with Mus81 and that Eme1 associates with Eme1. Thus, complexes containing two or more Mus81-Eme1 units could function to coordinate substrate cleavage in vivo. Down-regulation of Mus81 by RNA interference reduces mitotic recombination in human somatic cells. The recombination defect is rescued by expression of a bacterial Holliday junction resolvase. These data provide direct evidence for a role of Mus81-Eme1 in mitotic recombination in higher eukaryotes and support the hypothesis that Mus81-Eme1 resolves Holliday junctions in vivo.
Published: 2004

50. The Endogenous Mus81-Eme1 Complex Resolves Holliday Junctions by a Nick and Counternick Mechanism

Author: Paul Russell, Pierre-Henri L. Gaillard, Paul Shanahan, and Eishi Noguchi
Subjects: DNA Replication, Saccharomyces cerevisiae Proteins, Macromolecular Substances, Mutant, Cleavage (embryo), Gene Expression Regulation, Enzymologic, law.invention, Substrate Specificity, law, Gene Expression Regulation, Fungal, Schizosaccharomyces, Holliday junction, A-DNA, Molecular Biology, Polymerase, Cells, Cultured, biology, Fungal genetics, Proteins, DNA, Cell Biology, DNA Polymerase I, Endonucleases, MUS81, Molecular biology, DNA-Binding Proteins, Mutation, biology.protein, Recombinant DNA, Biophysics, Nucleic Acid Conformation, Schizosaccharomyces pombe Proteins
Abstract: Functional studies strongly suggest that the Mus81-Eme1 complex resolves Holliday junctions (HJs) in fission yeast, but in vitro it preferentially cleaves flexible three-way branched structures that model replication forks or 3' flaps. Here we report that a nicked HJ is the preferred substrate of endogenous and recombinant Mus81-Eme1. Cleavage occurs specifically on the strand that opposes the nick, resulting in resolution of the structure into linear duplex products. Resolving cuts made by the endogenous Mus81-Eme1 complex on an intact HJ are quasi-simultaneous, indicating that Mus81-Eme1 resolves HJs by a nick and counternick mechanism, with a large rate enhancement of the second cut arising from the flexible nature of the nicked HJ intermediate. Recombinant Mus81-Eme1 is ineffective at making the first cut. We also report that HJs accumulate in a DNA polymerase alpha mutant that lacks Mus81, providing further evidence that the Mus81-Eme1 complex targets HJs in vivo.
Published: 2003
Full Text: View/download PDF

Catalog

Books, media, physical & digital resources

See catalog results

Searchworks

Select search scope, currently: Articles Catalog books, media & more in Jio Institute collections Articles journal articles & other e-resources

Search

Search Constraints

Refine your results

Search Limiters

Topic

Publication Year Range

Language

Publication Type

Journal

Region

Database

Publisher

593 results on '"L. Gaillard"'

Search Results

Catalog

Select search scope, currently: Articles

Catalog

books, media & more in Jio Institute collections

Articles

journal articles & other e-resources