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Biological systems are subject to a dramatic increase in invading species in the 21st century due to increasing globalization around the world. In the near future, these species will cause more extensive ecological as well as socio-economic damage. Biotic invasions will result not only in a reduction of the original biodiversity, but also total loss, particularly degradation of natural habitats – especially Natura 2000 habitats. This study aims to understand the processes of spread of invasive plant species, their way of life, adaptation to environmental changes and displacement of native species. Without the local level, it is not possible to understand these processes on the regional and continental dimensions and thus implement scientific facts and findings into the prediction of the development of global biosystems. The research was conducted during the vegetation period in the years 2017–2021 in the studied areas of non-forest and forest habitats in the central part of Slovakia. We recorded 242 localities in the area with the occurrence of invasive plant species in an area of 3.057 km2 out of the total mapped area of 169.024 km2. We recorded the highest number of localities and the most expansive distribution for the species Stenactis annua, Robinia pseudoacacia, Solidago canadensis, Impatiens glandulifera and Fallopia japonica.

In this article we focus on the issue of determining the presence and status of the priority habitat 91I0* Euro-Siberian steppic woods with Quercus spp. in the South Slovak basin. As part of the issue, we try to verify the correctness of the procedure of the State Nature Conservancy of the Slovak Republic in the search for potential habitats and areas of European importance by converting the typological map to a map of habitats. Habitat 91I0* occurs in Slovakia in the form of three subtypes, namely Thermophilous and supra-Mediterranean oak woods ( Carpineto-Quercetum and Betuleto-Quercetum ), Acidophilous oak forests ( Quercetum ), while the last-named subtype is divided into two subunits: Medio-European acidophilous oak forests-part A and Pannonic hairy greenweed sessile oak woods-part B. Due to the current unsatisfactory state of the mentioned habitats, the requirement of the State Nature Conservancy of the Slovak Republic is to find and add new areas with the occurrence of habitat 91I0* in the south of Central Slovakia. During the mapping in the Lučenecká and Rimavská basins, greater emphasis was placed on the occurrence of the subtype Thermophilic Pontic-Pannonian oak forests on loess and sand, but its presence has not been confirmed. Subsequently, we focused on the search and identification of habitats in the model area, which is the area of European importance SKUEV0957 Uderinky. The result is a map of habitats in this area, which we then compare with a typological map, which determines the reliability of the converter used by the State Nature Conservancy of the Slovak Republic.

In the years 2018-2020, we carried out large-scale mapping in the Western Carpathians with a focus on determining the biodiversity of taxa of the family Orchidaceae using field biogeographical research. We evaluated the research using phytogeographic analysis with an emphasis on selected ecological environmental factors (substrate: ecological land unit value, soil reaction (pH), terrain: slope (°), flow and hydrogeological productivity (m 2 .s -1 ) and average annual amounts of global radiation (kWh.m -2 ). A total of 19 species were found in the area, of which the majority were Cephalenthera longifolia , Cephalenthera damasonium and Anacamptis morio . Rare findings included Epipactis muelleri , Epipactis leptochila and Limodorum abortivum . We determined the ecological demands of the abiotic environment of individual species by means of a functional analysis of communities. The research confirmed that most of the orchids that were studied occurred in acidified, calcified and basophil locations. From the location of the distribution of individual populations, it is clear that they are generally arranged compactly and occasionally scattered, which results in ecological and environmental diversity. During the research, we identified 129 localities with the occurrence of 19 species and subspecies of orchids. We identify the main factors that threaten them and propose specific measures to protect vulnerable populations.