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The article presents theoretical and experimental substantiation of relationship between the content of trace elements in the soil, water, feed and blood of mare with hypocobaltosis and hypocuprosis in biogeochemical province of Transcarpathia. The total content of Zn in soils is 50.96 mg/kg, Cu – 16.32, Co – 7.14, and Mn – 493.97 mg/kg. It was established decrease in the concentration of mobile forms of Cu and Co (0.01mg /kg), Cr (1.93), Pb (1.66), Ni (0.67), Zn (1.42). Also, in water from artesian wells of research farm it was established decreased content of Co (by 3.3 times), Cu (111), Zn (1.19), Mn (1.31), Cd (5.0 times) and on the contrary increased content of Pl by 5.6, Ni – 3.3 and Fe – 43.5 times. Providing of mares by Co is 54.7%, Cu – 88.5, Zn – 83.3, Fe – 88.4, Mn by 29%. The negative effect of Cobalt and Copper deficiency on erythrocytopoesis is shown. In particular, the number of erythrocytes, hemoglobin level, hematocrit value, and hemoglobin content in the erythrocyte indicate a development of alimentary anemia in pregnant mares. The established low level of Co and Cu that indicates the development of polymicroelementosis in mares. The Cobalt content in blood of non-pregnant mares was on the lower physiological limit and on average it was 0.48 ± 0.02 μmol/l. In the mares, at 7 month of pregnancy, the Cobalt content was lower (P < 0.01) than that of non-pegnant. In the 9–11 months of pregnancy, its level was on average 0.19 ± 0.02 μmol/l, which is lower by 2.5 and 1.9 times than in non-pregnant mares (P < 0.001) and at 4 months of pregnancy (P < 0.05). The Copper content in blood of mares at 9–11 months of pregnancy was lowest and the average was 2.09 ± 0.07 μmol/l (1.98–2.27), which is lower on 46.8 and 22.6% (P < 0.001) compared to non-pregnant and mares at 4 month of pregnancy. Hypocopperemia was established in 100% of studied mares. In non-pregnant mares, the Copper content was on average 3.93 ± 0.14 μmol/l. Taking into account the role of these microelements in metabolic transformations, changes in the composition of blood and the development of metabolism disorders in the organism of mares during the period of pregnancy can be explained.

The article presents the results of prophylactic effectiveness phytobiotic feed additive XTRACT™ 6930 for non-contagious diseases of the gastrointestinal tract in the composition of the basic diet of suckling pigs on modern pig farm. Diseases of the gastrointestinal tract make up from 40 to 60% of internal pathology in pigs. Used phytobiotic XTRACT™ 6930 with preventive measures, which had positive influence on oxygen-transport function of blood, hemoglobin synthesis and erythrocytopoesis in animals, as indicated by the number of erythrocytes, MCH and hematocrit in blood of suckling piglets from experimental group. Feeding of XTRACT™ 6930 had also positive influence on leukopoesis, as indicated by increased leukocyte rate in blood of suckling piglets from experimental group within physiological fluctuations. Increase of leukocyte rate can be caused by stimulation of non-specific resistance in piglets, which plays main role in defence of animals during early ontogenesis. As it is known, stabilization of phagocytic activity of leukocytes occurs during first months of age, when the body of pigs is able to synthesize most humoral factors of protection. Application of suckling piglets feed additive XTRACT™ 6930 for 18 days resulted in a positive impact on metabolism: increased levels of total protein, albumin, glucose serum; reduced levels of urea, phosphorus; reduced the activity of ALT, AST, ALP and GGT within physiological fluctuations. The use of phytobiotic feed additive XTRACT™ 6930 in experimental group of piglets increased the survival of piglets from experimental group in sucking period compared with control group of animals by reducing the percentage of morbidity and mortality from non-contagious diseases of the gastrointestinal tract. Consequently, it was established that the use XTRACT™ 6930 has positive effect on biochemical blood parameters of piglets, decreases their morbidity and mortality before weaning.

The inappropriate use of chemically synthesized antibacterial drugs, including fodder antibiotics, leads to a disturbance of the microbial ecosystems of the digestive canal and the appearance of pathogenic and opportunistic microorganisms of resistance to them. Therefore, in recent years, the use of animal-safe and environmentally friendly probiotics and phytobiotics has become widespread. Their action is aimed at the normalization of metabolic processes in the body and the mobilization of protective factors of the immune system, which is an effective alternative to traditional methods of prevention and treatment of diseases of the gastrointestinal tract. The article presents data on the influence of probiotic TOYOCERIN10⁹ and phytobiotic EXTRACT™ 6930 in combination with antibiotic (10% solution of enrofloxacin hydrochloride) on the clinical and haematological status of excised piglets, patients with gastroenteritis and their safety. The positive influence of probiotic and phytobiotic on the state of erythrocytopoesis was established. In particular, after treatment in experimental piglets, the number of erythrocytes decreased by 14.3% (P < 0.001) and 13.4% (P < 0.001), the hemoglobin content was 10.5% (P < 0.01) and 13.1% (P < 0.001) compared to the animals before treatment and reached the level of clinically healthy animals. The value of hematocrit in the blood of the exterminated piglets of all groups after treatment has reached the level of indices of clinically healthy animals. It is important to note that the use of probiotic and phytobiotic has positively influenced the leukocytopoiesis of piglets, as indicated by a decrease in the number of leukocytes in the blood of piglets in experimental groups by 26.9% (P < 0.001) and 25.4% (P < 0.001), compared with animal characteristics before treatment. Treatment of piglets using 10% solution of enrofloxacin hydrochloride, contributed to the improvement of the clinical condition of diseased animals in the 7–9th day, whereas in piglets, which were additionally used probiotic TOYOCERIN 10⁹ on 5–7th in the phytobiotic EXTRACT™ 6930 – on the 3–5th day. It was established that the use of probiotic TOYOCERIN 10⁹ and the phytobiotic EXTRACT™ 6930 in combination with the antibiotic increased the safety of experimental group piglets by 12.8% and 17.0%, as compared to the control group.