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1. Quantitative analysis of SERS spectra of MnSOD over fluctuated aptamer signals using multivariate statistics

Author

Yasukuni Ryohei, Gillibert Raymond, Triba Mohamed N., Grinyte Ruta, Pavlov Valery, and Lamy de la Chapelle Marc

Subjects
sers, sensor, mnsod, aptamer, multivariate analysis, Physics, QC1-999
Abstract

Surface-enhanced Raman scattering (SERS) sensors using specific aptamers often show difficulties in quantitative analysis because the instable aptamer structures show fluctuated background signals. In this communication, we address the quantitative analysis of the SERS spectra of manganese superoxide dismutase (MnSOD) in different concentrations over the signal arisen from its specific aptamer using multivariate statistical analysis. MnSOD is a primary antioxidant enzyme protecting normal tissue against oxidative stress and is known as a cancer biomarker. By applying principal component analysis, SERS spectra were distinguished when MnSOD was present in a specimen even at 10 pm. The relation between SERS spectra and MnSOD concentrations calculated by partial least-squares regression predicted MnSOD concentrations within one order of magnitude. Moreover, statistically obtained spectral correlations reveal that spectral differences did not originate from additional peaks of MnSOD but from the thermodynamic stability of the aptamer structures. These results open new paths for detection and analytical strategies of SERS-based bio-sensors using aptamers.

Published
2019
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14. Physical-chemical properties of DPPC liposomes in the presence of gold nanoparticles aggregates

Author

Pašalić, Lea, Bakarić, Danijela, Liu, Qiqian, and Lamy de la Chapelle, Marc

Subjects
liposomi, nanočestice zlata, spektroskopija, kalorimetrija
Abstract

Liposomes are lipid-based spherical structures that are, due to lipid amphiphilic character, arranged as bilayers with lipid hydrophobic tails oriented towards each other and a hydrophilic head towards aqueous surroundings. Because of their nontoxicity and biodegradability they represent powerful and efficient delivery drug systems. Nanoscience is the study of structures and molecules on the scales of nanometers ranging between 1 and 100 nm and represents an expanding and prominent research area. Metal nanoparticles (NPs), particularly those made of gold (Au NPs), attract great interest due to their stability, biocompatibility and unique catalytic activity. The interaction of Au NPs and liposomes came into research focus due to their diverse purpose in application, especially in nano- and biotechnology. Up to now, the understanding the interaction of liposomes and gold nanoparticles has gained great importance due to the possibilities that their combination can be used as potential drug delivery systems. [3] Here, we examined the influence of Au NPS aggregates on thermotropic properties of liposomes prepared from 1, 2-dipalmitoyl-snglycero-3-phosphocholine (DPPC). In order to reach this goal, our experimental work included synthesis of Au NPs, surface enhanced Raman scattering (SERS) measurement (Fig. 1a), confocal microscope imaging (Fig. 1b), temperature-dependent UV-Vis spectroscopy and differential scanning calorimetry (DSC).

Published
2023

15. Raman Tweezers for micro and nanoplastics analysis in watery ecosystems

Author

Foti, Antonino, Gillibert, Raymond, Magazzù, Alessandro, Donato, Maria Grazia, Maragò, Onofrio, Callegari, Agnese, Volpe, Giovanni, Lamy De La Chapelle, Marc, Lagarde, Fabienne, and Gucciardi Pietro, Giuseppe

Subjects
microplastics, raman spectroscopy, optical tweezers, tire and road wear particles, nanoplastics
Abstract

Hybrid instruments combining Optical Tweezers (OT) with Raman spectroscopy (Raman Tweezers, RT) are becoming increasingly popular, as they enable measurements of size and composition of micro and nanoparticles in a contact-less fashion. RTs are gaining attention as a unique analytical tool for environmental sciences and food analysis, capable to detect and chemically identify micro- and nano-plastics (MNPs). Here we show optical trapping and chemical identification of sub-20 μm plastics, down to the 50 nm range by RT [1]. Analysis at the single particle level allows us to unambiguously discriminate plastics from organic matter and mineral sediments, overcoming the capacities of standard Raman spectroscopy in liquid, intrinsically limited to ensemble measurements. Applications are demonstrated on both model particles and naturally aged environmental samples, made of common plastic pollutants, including polyethylene, polypropylene, nylon, and polystyrene, also in the presence of a thin eco-corona. Finally the analysis is extended to samples of tire and road wear particles collected from a brake test platform, where we highlight the presence of sub-micrometric agglomerates of rubber and brake debris, thanks to the presence of additional spectral features other than carbon [2]. Either used to trap & analyse single particles or coupled to field-flow fractionation techniques, RT have the potential to fill the technological gap in MNP detection. In the next years research & development will be oriented towards the exploration of the full potential and limits of RT in terms of sensitivity, size limitations, multispectral analysis and chemical information that can be extracted from contaminated MNPs and in complex environments. [3] [1] Gillibert et al. Environ. Sci. Technol. 2019, 53, 15, 9003 [2] Gillibert et al. Environ. Sci.: Nano, 2022, 9, 145 [3] Volpe et al. Roadmap for Optical Tweezers, arXiv preprint arXiv:2206.13789 (2022) Also see: https://micro2022.sciencesconf.org/425821/document, In MICRO 2022, Online Atlas Edition: Plastic Pollution from MACRO to nano

Published
2022
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17. Sensing performances of commercial SERS substrates

Author

AZZIZ Aicha, SAFAR Wafa, XIANG Yang, EDELY Mathieu, and LAMY de la CHAPELLE Marc

Subjects
Detection, SERS, Commercial substrates, 4-MBA, DNA, Sensor
Abstract

Since the discovery of Surface Enhanced Raman Spectroscopy (SERS) and the proven ability for this technique to detect molecules at trace level, numerous techniques have been reported for fabricating substrates that ensure reliable and reproducible SERS signal on its whole surface. In this paper we studied the capabilities and sensing performances of three commercial SERS substrates (RAM-SERS-SP from Ocean Optics, QSERS from Nanova Inc. and Hamamatsu from Hamamatsu Photonics) by using diluted solution of 4-mercaptobenzoic acid (MBA) at two different excitation wavelengths (633 and 785 nm). Large datasets were obtained through Raman mappings collected randomly on different areas on the substrate surface. The averaged SERS signals for one specific vibrational band of the MBA were used to compare limit of detection (LOD), limit of quantification (LOQ) and sensitivity of the three substrates. LOD and LOQ between 1 μM and 10 μM were calculated with a range of use of only one or two orders of magnitude. The best sensing performances were reached by the Hamamatsu substrate for an excitation wavelength of 633 nm.

Published
2022
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22. Raman tweezers for tire and road wear micro- and nanoparticles analysis

Author

Gillibert, Raymond, primary, Magazzù, Alessandro, additional, Callegari, Agnese, additional, Bronte-Ciriza, David, additional, Foti, Antonino, additional, Donato, Maria Grazia, additional, Maragò, Onofrio M., additional, Volpe, Giovanni, additional, Lamy de La Chapelle, Marc, additional, Lagarde, Fabienne, additional, and Gucciardi, Pietro G., additional

Published
2022
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24. Preface

Author

Chiș, Vasile, primary, Focșan, Monica, additional, Lamy de la Chapelle, Marc, additional, and Fausto, Rui, additional

Published
2021
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25. Raman Tweezers for Tire and Road Wear Micro- and Nanoparticles analysis

Author

Raymond, Gillibert, primary, Magazzù, Alessandro, additional, Callegari, Agnese, additional, Brente Ciriza, David, additional, Antonino, Foti, additional, Donato, Maria Grazia, additional, Maragò, Onofrio M., additional, Volpe, Giovanni, additional, Lamy de La Chapelle, Marc, additional, Lagarde, Fabienne, additional, and Gucciardi, Pietro Giuseppe, additional

Published
2021
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29. Raman Tweezers for Tire and Road Wear Micro- and Nanoparticles analysis

Author

gucciardi, pietro giuseppe, primary, Raymond, Gillibert, additional, Magazzù, Alessandro, additional, Callegari, Agnese, additional, Brente Ciriza, David, additional, Antonino, Foti, additional, Donato, Maria Grazia, additional, Maragò, Onofrio M., additional, Volpe, Giovanni, additional, Lamy de La Chapelle, Marc, additional, and Lagarde, Fabienne, additional

Published
2021
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30. Plasmonics-Nanofluidics Metamaterial: An Ultrasensitive Platform for Terahertz biosensing and Quantitative Measurement of Molecules

Author

Lamy de la Chapelle Marc, Jinchun He, Xia Ke, Fu Weiling, Xie Mingzhen, Zhang Yang, Mengya Li, Huiyan Tian, Xinyu Zhan, and Liu Yu

Subjects
Materials science, Terahertz radiation, Metamaterial, Nanotechnology, Nanofluidics, Biosensor, Plasmon
Abstract

Background: The terahertz spectroscopy has attracted enormous attention for label free chemical and biological sensing, due to the presence of molecular fingerprints and remote sensing capabilities. However, because of the strong absorption of THz waves in aqueous solutions, detecting liquid-phase biological samples by THz spectroscopy has encountered strong constraints. Results: Here, we demonstrate for the first time a rapid and label-free sensing for liquid-phase biological samples using THz spectroscopy based on microfluidic metasensors. The metasensors were integrated with microfluidic chip to precisely control the liquid sample thickness within 220 nanometer (MMS-N) and 50 micrometer (MMS-U). The metasensors include a periodic split ring resonator (SRR) with two resonant peaks in the THz range. The sensitivity(S) and corresponding figure of merit (FOM) of the two resonance peaks of MMS-N and MMS-U are analyzed with respect to different refractive index matching solution. Both the two resonance peaks of the metasensors are sensitive to the varies refractive index environment and high-frequency resonant of MMS-N shows the best sensitivity(~136GHz/RIU). In addition, the label-free biological sensing capability was conducted through qualitative and quantitative LDL and ox-LDL, where a shift of resonance frequency was observed as the LDL and ox-LDL concentration increased. Conclusions: The results indicated nanofluidic metasensor presented excellent sensing capability for liquid-phase molecular analysis by THz spectroscopy, and the proposed sensing system has the potentials to be developed as a rapid, label-free and highly sensitive detection tool for molecular analysis by THz sensing.

Published
2020
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33. New insight into the aptamer conformation and aptamer/protein interaction by surface-enhanced Raman scattering and multivariate statistical analysis

Author

Safar, Wafa, primary, Tatar, Andra-Sorina, additional, Leray, Aymeric, additional, Potara, Monica, additional, Liu, Qiqian, additional, Edely, Mathieu, additional, Djaker, Nadia, additional, Spadavecchia, Jolanda, additional, Fu, Weiling, additional, Derouich, Sarra Gam, additional, Felidj, Nordin, additional, Astilean, Simion, additional, Finot, Eric, additional, and Lamy de la Chapelle, Marc, additional

Published
2021
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37. Raman Tweezers for Small Microplastics and Nanoplastics Identification in Seawater

Author

Gillibert, Raymond, Balakrishnan, Gireeshkumar, Deshoules, Quentin, Tardivel, Morgan, Magazzu, Alessandro, Donato, Maria Grazia, Marago, Onofrio M., Lamy De La Chapelle, Marc, Colas, Florent, Lagarde, Fabienne, Gucciardi, Pietro G., Gillibert, Raymond, Balakrishnan, Gireeshkumar, Deshoules, Quentin, Tardivel, Morgan, Magazzu, Alessandro, Donato, Maria Grazia, Marago, Onofrio M., Lamy De La Chapelle, Marc, Colas, Florent, Lagarde, Fabienne, and Gucciardi, Pietro G.

Abstract

Our understanding of the fate and distribution of micro- and nano- plastics in the marine environment is limited by the intrinsic difficulties of the techniques currently used for the detection, quantification, and chemical identification of small particles in liquid (light scattering, vibrational spectroscopies, and optical and electron microscopies). Here we introduce Raman Tweezers (RTs), namely optical tweezers combined with Raman spectroscopy, as an analytical tool for the study of micro- and nanoplastics in seawater. We show optical trapping and chemical identification of sub-20 mu m plastics, down to the 50 nm range. Analysis at the single particle level allows us to unambiguously discriminate plastics from organic matter and mineral sediments, overcoming the capacities of standard Raman spectroscopy in liquid, intrinsically limited to ensemble measurements. Being a microscopy technique, RTs also permits one to assess the size and shapes of particles (beads, fragments, and fibers), with spatial resolution only limited by diffraction. Applications are shown on both model particles and naturally aged environmental samples, made of common plastic pollutants, including polyethylene, polypropylene, nylon, and polystyrene, also in the presence of a thin ecocorona. Coupled to suitable extraction and concentration protocols, RTs have the potential to strongly impact future research on micro and nanoplastics environmental pollution, and enable the understanding of the fragmentation processes on a multiscale level of aged polymers.

Published
2019
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38. Preface

Author

Chiș, Vasile, Focșan, Monica, Lamy de la Chapelle, Marc, and Fausto, Rui

Published
2022
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39. Gold Nanostructures for bimodal SPRI-SERS biosensors and study of their thermoplasmonic properties

Author

Bryche, J-F., Moreau, J., Maillart, E., Lamy de La Chapelle, Marc, Cloarec, Jp., Bartenlian, B., Canva., M., Laboratoire Nanotechnologies Nanosystèmes (LN2 ), Université de Sherbrooke (UdeS)-École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut Interdisciplinaire d'Innovation Technologique [Sherbrooke] (3IT), Université de Sherbrooke (UdeS), STMicroelectronics [Crolles] (ST-CROLLES), Service de Neurologie [CHU Pitié-Salpêtrière], IFR70-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut d'électronique fondamentale (IEF), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Sherbrooke (UdeS)-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), and LN2, UMI3463

Subjects
[SPI]Engineering Sciences [physics], [SPI] Engineering Sciences [physics], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

40. Innovative Gold Nanostructred biochips for surface plasmon resonance imaging and surface enhanced raman spectroscopy

Author

Bryche, J-F., Moreau, Julien, Barbillon, G., Sarkar, M., Maillart, E., Banville, F., Charette, P. G, Lamy de La Chapelle, Marc, Cloarec, J-P., Bartenlian, B., Canva., M., LN2, UMI3463, Laboratoire Nanotechnologies Nanosystèmes (LN2 ), Université de Sherbrooke (UdeS)-École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Institut Interdisciplinaire d'Innovation Technologique [Sherbrooke] (3IT), Université de Sherbrooke (UdeS), Laboratoire Charles Fabry / Biophotonique, Laboratoire Charles Fabry (LCF), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS), Service de Neurologie [CHU Pitié-Salpêtrière], IFR70-CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Institut d'électronique fondamentale (IEF), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Sherbrooke (UdeS)-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Université Paris-Sud - Paris 11 (UP11)-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS), and Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)

Subjects
[SPI]Engineering Sciences [physics], [SPI] Engineering Sciences [physics], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

41. Surface enhanced Raman scattering sensor for highly sensitive and selective detection of ochratoxin A

Author

Gillibert, Raymond, Triba, Mohamed, Lamy De La Chapelle, Marc, Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques (CSPBAT), and Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris 13 (UP13)-Institut Galilée-Université Sorbonne Paris Cité (USPC)

Subjects
[PHYS]Physics [physics], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

43. Raman Tweezers for Small Microplastics and Nanoplastics Identification in Seawater

Author

Gillibert, Raymond, primary, Balakrishnan, Gireeshkumar, additional, Deshoules, Quentin, additional, Tardivel, Morgan, additional, Magazzù, Alessandro, additional, Donato, Maria Grazia, additional, Maragò, Onofrio M., additional, Lamy de La Chapelle, Marc, additional, Colas, Florent, additional, Lagarde, Fabienne, additional, and Gucciardi, Pietro G., additional

Published
2019
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46. Raman Tweezers as a Tool for Small Microplastics and Nanoplastics Identification in Sea Water

Author

Gillibert, Raymond, primary, Balakrishnan, Gireeshkumar, additional, Deshoules, Quentin, additional, Tardivel, Morgan, additional, Magazzù, Alessandro, additional, Donato, Maria G., additional, Marago, Onofrio M., additional, Lamy de La Chapelle, Marc, additional, Colas, Florent, additional, Lagarde, Fabienne, additional, and Gucciardi, Pietro G., additional

Published
2019
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49. Next generation of Nanostructured Biochips for a bimodal SPRI-SERS detection

Author

Bryche, Jean-François, Moreau, Julien, Sarkar, Mitradeep, Olivéro, Aurore, Gillibert, Raymond, Maillart, Emmanuel, Hamouda, Frédéric, Aassime, Abdelhanin, Besbes, Mondher, Barbillon, Gregory, Gogol, Philippe, Laurenceau, Emmanuelle, Frydman, Chiraz, Chevolot, Yann, Lamy De La Chapelle, Marc, Cloarec, Jean-Pierre, Bartenlian, Bernard, Canva, Michael, Institut d'électronique fondamentale (IEF), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Charles Fabry / Biophotonique, Laboratoire Charles Fabry (LCF), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS)-Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)-Institut d'Optique Graduate School (IOGS), Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques (CSPBAT), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris 13 (UP13)-Institut Galilée-Université Sorbonne Paris Cité (USPC), HORIBA Scientific [France], INL - Chimie et Nanobiotechnologies (INL - C&N), Institut des Nanotechnologies de Lyon (INL), École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-École Centrale de Lyon (ECL), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Nanotechnologies Nanosystèmes (LN2 ), Université de Sherbrooke (UdeS)-École Centrale de Lyon (ECL), Université de Lyon-Université de Lyon-École supérieure de Chimie Physique Electronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Université Paris-Sud - Paris 11 (UP11)-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Institut d'Optique Graduate School (IOGS)-Centre National de la Recherche Scientifique (CNRS), and Université Paris 13 (UP13)-Institut Galilée-Université Sorbonne Paris Cité (USPC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[CHIM.MATE]Chemical Sciences/Material chemistry, [CHIM.OTHE]Chemical Sciences/Other, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

50. Phosphorylation impact on Spleen Tyrosine kinase conformation by Surface Enhanced Raman Spectroscopy

Author

Cottat, Maximilien, Yasukuni, Ryohei, Homma, Yo, Lidgi-Guigui, Nathalie, Varin-Blank, Nadine, Lamy de la Chapelle, Marc, Le Roy, Christine, Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques (CSPBAT), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Paris 13 (UP13)-Institut Galilée-Université Sorbonne Paris Cité (USPC), Université Paris 13 (UP13), Department of Applied Physics, Osaka University [Osaka], Adaptateurs de signalisation en hématologie (ASIH), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Sorbonne Paris Cité (USPC)-Université Paris 13 (UP13), Université Sorbonne Paris Cité (USPC)-Institut Galilée-Université Paris 13 (UP13)-Centre National de la Recherche Scientifique (CNRS), Université Paris 13 (UP13)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Paris 13 (UP13)-Institut Galilée-Université Sorbonne Paris Cité (USPC)-Centre National de la Recherche Scientifique (CNRS), and Université Paris 13 (UP13)-Institut Galilée-Université Sorbonne Paris Cité (USPC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Protein Conformation, [SDV]Life Sciences [q-bio], chemical and pharmacologic phenomena, hemic and immune systems, macromolecular substances, Protein Engineering, Spectrum Analysis, Raman, environment and public health, Article, enzymes and coenzymes (carbohydrates), Allosteric Regulation, Immune System, Humans, Syk Kinase, Phosphorylation, Allosteric Site, ComputingMilieux_MISCELLANEOUS, Signal Transduction
Abstract

Spleen Tyrosine Kinase (Syk) plays a crucial role in immune cell signalling and its altered expression or activation are involved in several cancers. Syk activity relies on its phosphorylation status and its multiple phosphorylation sites predict several Syk conformations. In this report, we characterized Syk structural changes according to its phosphorylation/activation status by Surface Enhanced Raman Spectroscopy (SERS). Unphosphorylated/inactive and phosphorylated/active Syk forms were produced into two expression systems with different phosphorylation capability. Syk forms were then analysed by SERS that was carried out in liquid condition on a lithographically designed gold nanocylinders array. Our study demonstrated that SERS signatures of the two Syk forms were drastically distinct, indicating structural modifications related to their phosphorylation status. By comparison with the atomic structure of the unphosphorylated Syk, the SERS peak assignments of the phosphorylated Syk nearest gold nanostructures revealed a differential interaction with the gold surface. We finally described a model for Syk conformational variations according to its phosphorylation status. In conclusion, SERS is an efficient technical approach for studying in vitro protein conformational changes and might be a powerful tool to determine protein functions in tumour cells.

Published
2017
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