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Objective: This study aimed to investigate the effects of different concentrations of preservatives on storage quality of orah fruits, so as to reduce the use of preservatives during its planting. Methods: The weight loss rate, vitamin C content, soluble solid content and titratable acid content of fruits were determined under different storage conditions, by using different concentrations of preservative. Results: At 4 ℃ and room temperature, the weight loss rate of fruits treated with preservatives was lower than that of the control group, and preservative treatment had different fresh-keeping effects on orah fruits. At 4 ℃, significant differences in vitamin C content among different treatments at 5, 28 and 42 days of storage were found, but no significant differences in other storage time. No significant difference in soluble solids and titratable acid contents among different treatments appeared. Among the treatment groups (treatment 1, treatment 2 and treatment 3), the weight loss rate, vitamin C content, soluble solid content and titratable acid content of orah fruit in treatment 2 were better than those in treatment 1 and 3. At room temperature, vitamin C content was significantly different among different treatments on the third day of storage. After 14 days of storage, the soluble solid content of treatment groups 1 and 2 was significantly different. On the 14th day of storage time, titratable acid content in treatment group 2 was significantly higher than that in treatment group 3. The contents of vitamin C, soluble solids and titratable acid in treatment group 2 were higher than those in other treatment groups, during most storage time. Conclusion: At 4 ℃, each preservative treatment has different degrees of preservation effect on orah fruits. 450 g/L midazamine +500 g/kg statin +100 g/kg 2,4-D+400 g/kg bellkute has better preservation effect on orah fruit.

Objective *To explore the differential gene modules and hub genes of major depressive disorder (MDD) by weighted gene co-expression network analysis (WGCNA), and annotate the biological functions of the differential gene modules. Methods *Based on the previous experiment of peripheral blood mRNA microarray analysis of 8 MDD patients and 8 healthy controls (control group), t-test statistical method was used to screen the differentially expressed genes between MDD and the control group. WGCNA package in R software was used to analyze the weighted gene co-expression network. The correlation coefficient threshold was set to 0.9 with βi =14 to construct the co-expression network of the gene dataset. The hybrid dynamic tree cutting method was used to cut the network to generate gene modules. Pearson correlation test was used to evaluate the correlation between gene modules and MDD. The gene modules with the strongest positive correlation and the strongest negative correlation with depression were selected respectively, and the top three genes with the highest connectivity in the modules were defined as hub genes. Finally, GO functional enrichment analysis and KEGG pathway analysis were used to annotate the functions of the two modules. Results *A total of 4 125 differentially expressed genes were obtained from 16 samples, and 9 gene modules were identified. The blue module (R= - 0.91, P=0.000) and the cyan module (R=0.76, P=0.000) were selected for further study. The hub genes of blue module were JAM2 (junctional adhesion molecule 2), SCRN2 (secernin 2) and IGHV7-81 (immunoglobulin heavy variable 7-81). The hub genes of cyan module were SCFD2 (Sec1 family domain containing 2), NR5A2 (nuclear receptor subfamily 5 group A member 2) and KCNMA1 (potassium calcium-activated channel subfamily M alpha 1). It was found in the annotation of biological function that the genes of the cyan module were mainly enriched in the embryo development, cell growth and immune and inflammatory response, while the genes of the blue module were mainly enriched in material processing and transport and infection. Conclusion *Two peripheral blood mRNA gene modules and six hub genes (JAM2, SCRN2, IGHV7-81, SCFD2, NR5A2 and KCNMA1) may be significantly associated with MDD. These two gene modules may play a role in the embryonic development, immune and inflammatory response, and material processing and transportation. [ABSTRACT FROM AUTHOR]