1. Degradation of nanoRNA is performed by multiple redundant RNases in Bacillus subtilis
- Author
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Ciarán Condon, Undine Mechold, Antoine Danchin, Vasily Ogryzko, Ming Fang, Wencke-Maria Zeisberg, Génétique des Génomes Bactériens, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie physico-chimique (IBPC (FR_550)), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Interactions moléculaires et cancer (IMC (UMR 8126)), Signalisation, noyaux et innovations en cancérologie (UMR8126), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Centre National de la Recherche Scientifique (CNRS), European Union Network of Excellence BioSapiens[LSHG CT-2003-503265 to A.D.], the French ResearchAgency (Agence nationale de la recherche) [BLAN06-3_135068 toC.C.], La Ligue Contre le Cancer[9ADO1217/1B1-BIOCE to V.O.], Institut national duCancer [247343/1B1-BIOCE to V.O.]. Funding for openaccess charge: Institut Pasteur., Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], and Centre National de la Recherche Scientifique (CNRS)
- Subjects
MESH: Ribonucleases ,MESH: Mutation ,RNase P ,Mutant ,Bacillus subtilis ,Biology ,medicine.disease_cause ,03 medical and health sciences ,Ribonucleases ,MESH: RNA ,Genetics ,medicine ,Genomic library ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,MESH: Phylogeny ,Escherichia coli ,Phylogeny ,030304 developmental biology ,0303 health sciences ,MESH: Genetic Complementation Test ,Oligoribonucleotides ,Nucleic Acid Enzymes ,030306 microbiology ,Genetic Complementation Test ,fungi ,MESH: DNA ,RNA ,DNA ,respiratory system ,MESH: Bacillus subtilis ,biology.organism_classification ,Molecular biology ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,Complementation ,Biochemistry ,Mutation ,MESH: Oligoribonucleotides ,sense organs ,Exoribonuclease activity - Abstract
International audience; Escherichia coli possesses only one essential oligoribonuclease (Orn), an enzyme that can degrade oligoribonucleotides of five residues and shorter in length (nanoRNA). Firmicutes including Bacillus subtilis do not have an Orn homolog. We had previously identified YtqI (NrnA) as functional analog of Orn in B. subtilis. Screening a genomic library from B. subtilis for genes that can complement a conditional orn mutant, we identify here YngD (NrnB) as a second nanoRNase in B. subtilis. Like NrnA, NrnB is a member of the DHH/DHHA1 protein family of phosphoesterases. NrnB degrades nanoRNA 5-mers in vitro similarily to Orn. Low expression levels of NrnB are sufficient for orn complementation. YhaM, a known RNase present in B. subtilis, degrades nanoRNA efficiently in vitro but requires high levels of expression for only partial complementation of the orn(-) strain. A triple mutant (nrnA(-), nrnB(-), yhaM(-)) in B. subtilis is viable and shows almost no impairment in growth. Lastly, RNase J1 seems also to have some 5'-to-3' exoribonuclease activity on nanoRNA and thus can potentially finish degradation of RNA. We conclude that, unlike in E. coli, degradation of nanoRNA is performed in a redundant fashion in B. subtilis.
- Published
- 2009