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296 results on '"Laboratoire de Biotechnologie et Chimie Marines (LBCM)"'

1. Extracts from Wallis Sponges Inhibit Vibrio harveyi Biofilm Formation

Author

Caudal, Flore, Rodrigues, Sophie, Dufour, Alain, Artigaud, Sébastien, Le Blay, Gwénaëlle, Petek, Sylvain, Bazire, Alexis, Laboratoire de Biotechnologie et Chimie Marine (LBCM), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
sponge extract, anti-biofilm activity, [SDV]Life Sciences [q-bio], marine natural products, Vibrio harveyi, biofilm
Abstract

International audience; Pathogenic bacteria and their biofilms are involved in many human and animal diseases and are a major public health problem with, among other things, the development of antibiotic resistance. These biofilms are known to induce chronic infections for which classical treatments using antibiotic therapy are often ineffective. Sponges are sessile filter-feeding marine organisms known for their dynamic symbiotic partnerships with diverse microorganisms and their production of numerous metabolites of interest. In this study, we investigated the antibiofilm efficacy of different extracts from sponges, isolated in Wallis, without biocidal activity. Out of the 47 tested extracts, from 28 different genera, 11 showed a strong activity against Vibrio harveyi biofilm formation. Moreover, one of these extracts also inhibited two quorum-sensing pathways of V. harveyi.

Published
2023
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2. Identification of the quorum sensing signal of the opportunistic pathogen inducing bleaching disease in the red macroalga Halymenia floresii holobiont

Author

Shareen A Abdul Malik, Mahasweta Saha, Laure Taupin, Gilles Bedoux, Nathalie Bourgougnon, Daniel Robledo, Laboratoire de Biotechnologie et Chimie Marine (LBCM), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV]Life Sciences [q-bio], General Engineering
Abstract

Seaweed surface provides a suitable substratum for the settlement of microorganisms (bacteria, viruses, and plankton). These microbial partners may have either beneficial or detrimental effects on the host since surface microbiota can act either as a protective layer to the macroalgae (symbiotic beneficial relations) or, under changing environmental conditions, the microbial equili-brium may shift to a detrimental, pathogenic state thus inducing diseases in the host. In commer-cial aquaculture, seaweed diseases feature a growing concern. Pathogenicity of microorganisms that infect algal hosts is closely related to the release of virulence factors and the formation of biofilms, both of which are regulated by Quorum Sensing (QS). The main focus of this study is to determine the pathogenicity of the surface-associated bacteria of Halymenia floresii, a carrageenophyte that originated from distinct habitats. Twenty-five bacterial species isolated from the surface of H. floresii were individually tested for tip bleaching assay to evaluate their potential pathogenicity. Ten isolates significantly reduced the risk of tip bleaching in H. floresii and were designated as “significantly non-pathogenic”. Vibrio owensii was identified as a “significant pathogen” inducing bleaching disease in H. floresii. By using LC-MS, we here identified its HomoSerine Lactones (HSL) QS signal, as a C4-HSL (short-chain). This study thus suggests a possible involvement of QS signal (short-chain) in the disease-inducing bacterium from the aquaculture ponds (an integrated multitrophic aquaculture system). This study firstly reports on the surface-associated bacteria of a lambda-carrageenophyte. This study must contribute to the development of dedicated strategies for disease control based on HSL disruption in aquaculture.

Published
2022
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3. Monitoring de la croissance de bactéries / biofilms bactériens via une sonde coaxiale

Author

Longo, Matthieu, Rioual, Stéphane, Talbot, Philippe, Fay, Fabienne, Hellio, Claire, Lescop, Benoît, Equipe Smart Materials and Related Technologies (Lab-STICC_SMART), Laboratoire des sciences et techniques de l'information, de la communication et de la connaissance (Lab-STICC), École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-IMT Atlantique (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-IMT Atlantique (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT), Laboratoire de Biotechnologie et Chimie Marine (LBCM), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDE.IE]Environmental Sciences/Environmental Engineering, [SDE]Environmental Sciences
Abstract

International audience;  Le but de ce travail est de développer une sonde radiofréquence pour le suivi du développement de bactéries et/ou de biofilm bactérien. Il s'agit d'une sonde coaxiale ouverte plongée dans un réservoir rempli d'une solution. Des simulations HFSS ont été effectuées afin de vérifier la faisabilité de la sonde. Le suivi de la prolifération de deux bactéries modèles a été testé avec succès. La détermination de la présence d'un biofilm est aussi possible. Ces suivis peuvent être obtenus à une fréquence unique permettant un suivi temps réel à faible coût.

Published
2023

4. The absence of the Pseudomonas aeruginosa OprF protein leads to increased biofilm formation through variation in c-di-GMP level

Author

Alain Dufour, Olivier Lesouhaitier, Joerg Overhage, Gwendoline Gicquel, Gerald Brenner-Weiss, Alexis Bazire, Joana A. Moscoso, Olivier Maillot, Rachel Duchesne, Alain Filloux, Sylvie Chevalier, Nicole Orange, Thibaut Rosay, Magalie Bénard, Patrice Lerouge, Marc G. J. Feuilloley, Emeline Bouffartigues, Laurène Fito-Boncompte, Laboratoire de Microbiologie Signaux et Microenvironnement (LMSM), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Imperial College London, Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie (PRIMACEN), Normandie Université (NU)-Normandie Université (NU)-Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Karlsruhe Institute of Technology (KIT), Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Laboratoire de Biotechnologie et Chimie Marine (LBCM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-High-tech Research Infrastructures for Life Sciences (HeRacLeS), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Bazire, Alexis, Laboratoire de Microbiologie du Froid – Signaux et Micro-Environnement ( LMDF-SME ), Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Normandie Université ( NU ), Reproduction et développement des plantes ( RDP ), Centre National de la Recherche Scientifique ( CNRS ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique ( INRA ) -École normale supérieure - Lyon ( ENS Lyon ), Laboratoire de Biotechnologie et Chimie Marines ( LBCM ), Université de Bretagne Sud ( UBS ) -Université de Brest ( UBO ), Institute for Research and Innovation in Biomedicine ( IRIB ), Normandie Université ( NU ) -Normandie Université ( NU ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Karlsruhe Institute of Technology ( KIT ), Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale ( Glyco-MEV ), UR DER Développement et économie rurale, Institut Supérieur d'Agriculture Rhône-Alpes, Laboratoire Interdisciplinaire Carnot de Bourgogne ( LICB ), Université de Bourgogne ( UB ) -Centre National de la Recherche Scientifique ( CNRS ), and Biotechnology and Biological Sciences Research Council (BBSRC)

Subjects
SIGMA-FACTOR SIGX, EXPRESSION, Microbiology (medical), OUTER-MEMBRANE PROTEIN, Mutant, lcsh:QR1-502, Biology, [ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, medicine.disease_cause, Microbiology, lcsh:Microbiology, biofilm, Sigma factor, medicine, ECF, NATURAL sciences & mathematics, ComputingMilieux_MISCELLANEOUS, Original Research, Science & Technology, CYSTIC-FIBROSIS, ROLES, RECEPTOR, Pseudomonas aeruginosa, exopolysaccharides, Biofilm, OprF, SigX, c-di-GMP, biochemical phenomena, metabolism, and nutrition, EXOPOLYSACCHARIDE PRODUCTION, GENE, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Cell aggregation, POLYSACCHARIDE, Regulon, Porin, ddc:500, [SDV.MP.BAC] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Bacterial outer membrane, Life Sciences & Biomedicine, MATRIX
Abstract

OprF is the major outer membrane porin in bacteria belonging to the Pseudomonas genus. In previous studies, we have shown that OprF is required for full virulence expression of the opportunistic pathogen Pseudomonas aeruginosa. Here, we describe molecular insights on the nature of this relationship and report that the absence of OprF leads to increased biofilm formation and production of the Pel exopolysaccharide. Accordingly, the level of c-di-GMP, a key second messenger in biofilm control, is elevated in an oprF mutant. By decreasing c-di-GMP levels in this mutant, both biofilm formation and pel gene expression phenotypes were restored to wild-type levels. We further investigated the impact on two small RNAs, which are associated with the biofilm lifestyle, and found that expression of rsmZ but not of rsmY was increased in the oprF mutant and this occurs in a c-di-GMP-dependent manner. Finally, the extracytoplasmic function (ECF) sigma factors AlgU and SigX displayed higher activity levels in the oprF mutant. Two genes of the SigX regulon involved in c-di-GMP metabolism, PA1181 and adcA (PA4843), were up-regulated in the oprF mutant, partly explaining the increased c-di-GMP level. We hypothesized that the absence of OprF leads to a cell envelope stress that activates SigX and results in a c-di-GMP elevated level due to higher expression of adcA and PA1181. The c-di-GMP level can in turn stimulate Pel synthesis via increased rsmZ sRNA levels and pel mRNA, thus affecting Pel-dependent phenotypes such as cell aggregation and biofilm formation. This work highlights the connection between OprF and c-di-GMP regulatory networks, likely via SigX (ECF), on the regulation of biofilm phenotypes.

Published
2015
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5. Brown Seaweed Sargassum-Based Sorbents for the Removal of Cr(III) Ions from Aqueous Solutions

Author

Natalia Niedzbała, Katarzyna Dziergowska, Maja Wełna, Anna Szymczycha-Madeja, Jacek Chęcmanowski, Nathalie Bourgougnon, Izabela Michalak, Laboratoire de Biotechnologie et Chimie Marine (LBCM), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Process Chemistry and Technology, seaweed, Cr(III) ions sorption, [SDE]Environmental Sciences, nanoparticles biosynthesis, extraction, Chemical Engineering (miscellaneous), Bioengineering, wastewater
Abstract

International audience; In this study, zinc oxide nanoparticles (ZnO NPs) were biosynthesized with the use of an extract derived from seaweed (Sargassum sp.) and used as a sorbent for the removal of Cr(III) ions from wastewater. The biosorption properties of the seaweed itself as well as of the post-extraction residue were investigated for comparison. ZnO NPs were characterized with UV–vis, ICP-OES, FTIR, XRD, and SEM techniques. The sorption capacity of the (bio)sorbents was investigated as a function of contact time at different pH values and initial concentrations of metal ions. Sorption kinetics and isotherms were studied in order to comprehend the sorption nature and mechanism. The sorption kinetic data were well-fitted with the pseudo-second-order model, and the highest sorption capacity was calculated for ZnO NPs (137 mg/g), whereas those calculated for Sargassum sp. (82.0 mg/g) and the post-extraction residue (81.3 mg/g) were comparable (at pH 5 and 300 mg of Cr(III) ions/L). The adsorption isotherms for all sorbents were well described using the Langmuir model. According to these findings, ZnO NPs were superior to the sorption properties of the tested biosorbents and can be used as a potential sorbent for the removal of metal ions from wastewater. Renewable seaweed biomass can be used for the sustainable biosynthesis of nanoparticles used for environmental protection.

Published
2023
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6. Possible planet formation in the young, low-mass, multiple stellar system GG Tau A

Author

Tracy L. Beck, E. Chapillon, Ya-Wen Tang, Anne Dutrey, Stéphane Guilloteau, Jeffrey S. Bary, Vincent Piétu, Hervé Beust, Frederic Gueth, Arnaud Pierens, J.-M. Huré, Yann Boehler, Michal Simon, Emmanuel Di Folco, AMOR 2014, Laboratoire d'astrodynamique, d'astrophysique et d'aéronomie de bordeaux (L3AB), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire d'Astrophysique de Bordeaux [Pessac] (LAB), Université de Bordeaux (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bordeaux (UB), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Astrophysique de Bordeaux [Pessac] (LAB), Université de Bordeaux (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux (UB), Centro de Radioastronomia y Astrofisica, Universidad Nacional Autónoma de México (UNAM), Department of Physics and Astronomy [Hamilton NY], Colgate University, Space Telescope Science Institute (STSci), Institut de Planétologie et d'Astrophysique de Grenoble (IPAG), Centre National d'Études Spatiales [Toulouse] (CNES)-Université Grenoble Alpes (UGA)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire des Sciences de l'Univers de Grenoble (OSUG), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Institut de RadioAstronomie Millimétrique (IRAM), Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université de Bretagne Sud (UBS), Academia Sinica Institute of Astronomy and Astrophysics (ASIAA), Academia Sinica, Centro de Radioastronomia y Astrofisica (CRyA), Institut de Planétologie et d'Astrophysique de Grenoble (IPAG ), Observatoire des Sciences de l'Univers de Grenoble (OSUG), Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire d'Astrophysique de Bordeaux [Pessac] (LAB), Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Universidad Nacional Autónoma de México = National Autonomous University of Mexico (UNAM), Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP )-Institut national des sciences de l'Univers (INSU - CNRS)-Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'astrodynamique, d'astrophysique et d'aéronomie de bordeaux ( L3AB ), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers ( INSU - CNRS ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers ( INSU - CNRS ) -Centre National de la Recherche Scientifique ( CNRS ) -Observatoire aquitain des sciences de l'univers ( OASU ), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers ( INSU - CNRS ) -Centre National de la Recherche Scientifique ( CNRS ) -Centre National de la Recherche Scientifique ( CNRS ) -Laboratoire d'Astrophysique de Bordeaux [Pessac] ( LAB ), Université de Bordeaux ( UB ) -Institut national des sciences de l'Univers ( INSU - CNRS ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Bordeaux ( UB ), Observatoire aquitain des sciences de l'univers ( OASU ), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers ( INSU - CNRS ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire d'Astrophysique de Bordeaux [Pessac] ( LAB ), Université de Bordeaux ( UB ) -Institut national des sciences de l'Univers ( INSU - CNRS ) -Centre National de la Recherche Scientifique ( CNRS ), Université de Bordeaux ( UB ), Universidad Nacional Autónoma de México ( UNAM ), Space Telescope Science Institute ( STSci ), Institut de Planétologie et d'Astrophysique de Grenoble ( IPAG ), Observatoire des Sciences de l'Univers de Grenoble ( OSUG ), Université Joseph Fourier - Grenoble 1 ( UJF ) -Institut national des sciences de l'Univers ( INSU - CNRS ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Grenoble Alpes ( UGA ) -Université Joseph Fourier - Grenoble 1 ( UJF ) -Institut national des sciences de l'Univers ( INSU - CNRS ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Grenoble Alpes ( UGA ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de RadioAstronomie Millimétrique ( IRAM ), Centre National de la Recherche Scientifique ( CNRS ), Laboratoire de Biotechnologie et Chimie Marines ( LBCM ), Université de Bretagne Sud ( UBS ) -Université de Brest ( UBO ), and Institute of Astronomy and Astrophysics [Taipei] ( ASIAA )

Subjects
Physics, Multidisciplinary, 010504 meteorology & atmospheric sciences, [SDU.ASTR.CO]Sciences of the Universe [physics]/Astrophysics [astro-ph]/Cosmology and Extra-Galactic Astrophysics [astro-ph.CO], [ PHYS.ASTR ] Physics [physics]/Astrophysics [astro-ph], Exoplanets, Astronomical unit, Astronomy, Astrophysics, 01 natural sciences, Accretion (astrophysics), Exoplanet, Stars, Planet, [ SDU.ASTR.CO ] Sciences of the Universe [physics]/Astrophysics [astro-ph]/Cosmology and Extra-Galactic Astrophysics [astro-ph.CO], 0103 physical sciences, Binary star, Astrophysics::Solar and Stellar Astrophysics, Astrophysics::Earth and Planetary Astrophysics, Circumbinary planet, Low Mass, [PHYS.ASTR]Physics [physics]/Astrophysics [astro-ph], 010303 astronomy & astrophysics, Astrophysics::Galaxy Astrophysics, 0105 earth and related environmental sciences
Abstract

Investigation of the triple stellar system GG Tau A reveals gas fragments within the central cavity between the Keplerian outer ring orbiting the entire system and the stars themselves; gas flow from this outer ring appears capable of sustaining the inner disk surrounding component star GG Tau Aa beyond the accretion lifetime, leaving time for planet formation to occur. Atacama Large Millimetre Array (ALMA) observations of G GTau A, one of the most intensively studied stellar systems, reveal a flow of fresh material that would make planet formation possible even in the unstable environment of young binary star systems. This flow had previously been predicted by numerical simulation, but not confirmed observationally. New images show gas fragments traced by carbon monoxide emission within the GG Tau A cavity. Kinematic analysis suggests that the flow is capable of sustaining the inner disk around GG Tau Aa beyond the accretion lifetime, leaving sufficient time to allow planet formation. The formation of planets around binary stars may be more difficult than around single stars1,2,3. In a close binary star (with a separation of less than a hundred astronomical units), theory predicts the presence of circumstellar disks around each star, and an outer circumbinary disk surrounding a gravitationally cleared inner cavity around the stars4,5. Given that the inner disks are depleted by accretion onto the stars on timescales of a few thousand years, any replenishing material must be transferred from the outer reservoir to fuel planet formation (which occurs on timescales of about one million years). Gas flowing through disk cavities has been detected in single star systems6. A circumbinary disk was discovered around the young low-mass binary system GG Tau A (ref. 7), which has recently been shown to be a hierarchical triple system8. It has one large inner disk9 around the single star, GG Tau Aa, and shows small amounts of shocked hydrogen gas residing within the central cavity10, but other than a single weak detection11, the distribution of cold gas in this cavity or in any other binary or multiple star system has not hitherto been determined. Here we report imaging of gas fragments emitting radiation characteristic of carbon monoxide within the GG Tau A cavity. From the kinematics we conclude that the flow appears capable of sustaining the inner disk (around GG Tau Aa) beyond the accretion lifetime, leaving time for planet formation to occur there. These results show the complexity of planet formation around multiple stars and confirm the general picture predicted by numerical simulations.

Published
2014
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7. Design of Polyhydroxyalkanoate (PHA) Microbeads with Tunable Functional Properties and High Biodegradability in Seawater

Author

Eric Balnois, Anthony Magueresse, Chloé Volant, Stéphane Bruzaud, Guillaume Vignaud, Laboratoire de Biotechnologie et Chimie Marine (LBCM), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
chemistry.chemical_classification, Materials science, Environmental Engineering, Polymers and Plastics, Polymer, 02 engineering and technology, Biodegradation, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, Polyhydroxyalkanoates, Amorphous solid, 0104 chemical sciences, chemistry.chemical_compound, chemistry, Polylactic acid, Chemical engineering, Surface roughness, Materials Chemistry, Degradation (geology), [CHIM]Chemical Sciences, sense organs, Cellulose, 0210 nano-technology
Abstract

Commercial poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBHV) and poly(3-hydroxybutyrate-co-3-hydroxyhexanoate) (PHBHHx) were used to prepare microbeads, with diameter ranging from 50 to 100 µm, by an emulsion-evaporation process. The properties of the beads reveal that the elaboration process enables the formation of spherical particles, that the crystallinity of the former polymer is not altered during the process and that the surface roughness of the particles can be tuned by changing the nature of the lateral chain in the PHA structure, in good correlation with its crystalline behavior. The mechanical properties of the different PHA beads are also found to be intimely linked with the crystalline content of the beads, with modulus varying between 1 to 7 GPa. All these properties are also governing the degradation behavior of these materials, as tested under marine environment. With a rapid degradation, similar to cellulose, and a degradation rate correlated with the crystalline content, these results emphasize the interest in developing PHA materials with tunable functions and degradation properties.

Published
2021
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8. Dignostic écotoxicologique de la pollution marine au Sénégal

Author

Sonko, Amidou, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Bretagne Sud, Université Cheikh Anta Diop (Dakar), Isabelle Linossier, Gaël Le Pennec, Mamadou Fall, and Patrice Brehmer

Subjects
Trace metals, Plastic pollution, Microbiological parameters, Paramètres microbiologiques, Sociological survey, Marine pollution, [INFO.INFO-BT]Computer Science [cs]/Biotechnology, Bioassays, Bio-essais
Abstract

We studied the marine pollution of the sediments and coastal waters of the Cape Verde peninsula and the Petite Côte. The results show that the perception of the population is not always in line with scientific observations. The population reports a mainly moderate level of pollution, which has worsened over the last ten years. The majority of the sites studied do not comply with the microbiological quality of bathing water from a microbiological point of view. The quantities of microplastics at some sites are high. Macroplastics at sea are not significantly present at all the sites sampled. For TMEs only chromium and nickel are above the probable effect concentration (PEC) on marine organisms for the fraction ≤ 100 μm. The first trophic levels of the marine food chain do not seem to be affected by sediment toxicity, whereas the middle and upper levels are more affected. The majority (81%) of the sites studied revealed a level of ecotoxicity greater than 20%, via the Magallana gigas embryotoxicity test, which is known to be more sensitive than other bioassays. Consequently, the assessment of the toxicity of marine sediments shows that the majority of the selected sites appear to be in a poor ecotoxicological state. The inter-site variability of the results is due to the characteristics of the sites.; Nous avons étudié la pollution marine des sédiments et des eaux côtières de la presqu’ile du Cap-Vert et de la Petite côte. Les résultats montrent que la perception des populations n’est pas toujours en phase avec les observations scientifiques. La population signale un niveau de pollution principalement modérée mais qui s’est aggravé ces dix dernières années. La majorité des sites étudiés n’est pas conforme aux qualités microbiologiques de l’eau de baignade d’un point de vu microbiologique. Les quantités de microplastiques de certains sites sont élevées. Les macroplastiques en mer, ne sont pas significativement présents sur tous les sites échantillonnés. Pour les ETM seuls le chrome et le nickel sont au-dessus de la concentration d'effet probable (CEP) sur les organismes marins en ce qui concerne la fraction ≤ 100 μm. Les premiers niveaux trophiques de la chaine alimentaire du milieu marin ne semblent pas être affectés par la toxicité des sédiments alors que les niveaux moyens et supérieurs le sont davantage. La majorité (81%) des sites étudiés ont révélé un niveau d’écotoxicité supérieur à 20%, via le test d’embryotoxicité de Magallana gigas reconnu pour sa plus grande sensibilité par rapport aux autres bioessais. Par conséquent, l'évaluation de la toxicité des sédiments marins montre que la majorité des sites sélectionnés apparaissent dans un mauvais état écotoxicologique. La variabilité inter-sites des résultats sont dues aux caractéristiques des sites.

Published
2022

9. Biofuel Production from Seaweeds: A Comprehensive Review

Author

Yiru Zhao, Nathalie Bourgougnon, Jean-Louis Lanoisellé, Thomas Lendormi, Laboratoire de Biotechnologie et Chimie Marine (LBCM), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Control and Optimization, Renewable Energy, Sustainability and the Environment, [SDV]Life Sciences [q-bio], Energy Engineering and Power Technology, Building and Construction, Electrical and Electronic Engineering, Engineering (miscellaneous), Energy (miscellaneous)
Abstract

International audience; Seaweeds represent a promising and sustainable feedstock for biofuel production which raises increasing research interests. Their high availability, easy fermentable composition, and good degradation potential make them a suitable candidate for alternating fossil fuels as an advantageous energy resource. This comprehensive review aims to summarize and discuss data from the literature on the biochemical composition of seaweeds and its potential for biomethane and biohydrogen production, as well as to investigate the effect of the common pretreatment methods. Satisfactory yields comparable to terrestrial biomass could be obtained through anaerobic digestion; concerning dark fermentation, the challenge remains to better define the operating conditions allowing a stable production of biohydrogen. Finally, we propose a potential energy production scheme with the seaweed found by the Caribbean Islands of Guadeloupe and Martinique, as well as current techno-economic challenges and future prospects. An annual energy potential of 66 GWh could be attained via a two-stage biohythane production process, this tends to be promising in terms of energetic valorization and coastal management.

Published
2022
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10. Genetic and physiological insights into the diazotrophic activity of a non‐cyanobacterial marine diazotroph

Author

Aurélie Joublin‐Delavat, Katia Touahri, Pauline Crétin, Amandine Morot, Sophie Rodrigues, Bruno Jesus, Florian Trigodet, François Delavat, Unité en Sciences Biologiques et Biotechnologies de Nantes (US2B), Centre National de la Recherche Scientifique (CNRS)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE), Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ), Institut de Chimie de Strasbourg, Université de Strasbourg (UNISTRA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Des Substances et Organismes de la Mer - UR 2160 (ISOMER), Nantes Université - UFR des Sciences Pharmaceutiques et Biologiques (Nantes Univ - UFR Pharmacie), Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes université - UFR des Sciences et des Techniques (Nantes univ - UFR ST), Nantes Université - pôle Sciences et technologie, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Sciences et technologie, Nantes Université (Nantes Univ), and The University of Chicago Medicine [Chicago]

Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology, Nitrogen, Nitrogen Fixation, Oceans and Seas, Humans, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Noncommunicable Diseases, Cyanobacteria, Microbiology, Ecology, Evolution, Behavior and Systematics
Abstract

Nitrogen (N2) fixation, or diazotrophy, supports a large part of primary production in oceans. Culture-independent approaches highlighted the presence in abundance of marine non-cyanobacterial diazotrophs (NCD) but their ecophysiology remains elusive, mostly because of the low number of isolated NCD and because of the lack of available genetic tools for these isolates. Here, a dual genetic and functional approach allowed unveiling the ecophysiology of a marine NCD affiliated to the species Vibrio diazotrophicus. Physiological characterization of the first marine NCD mutant obtained so far was performed using a soft-gellan assay, demonstrating that a ΔnifH mutant in not able to grow in nitrogen-deprived media. Furthermore, we demonstrated that V. diazotrophicus produces a thick biofilm under diazotrophic conditions, suggesting biofilm production as an adaptive response of this NCD to cope with the inhibition of nitrogen-fixation by molecular oxygen. Finally, the genomic signature of V. diazotrophicus is essentially absent from metagenomic data of Tara Ocean expeditions, despite having been isolated from various marine environments. We think that the genetically tractable V. diazotrophicus strain used in this study may serve as an ideal model to study the ecophysiology of these overlooked procaryotic group.

Published
2022
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11. Anti-Biofilm Activity of a Hyaluronan-like Exopolysaccharide from the Marine Vibrio MO245 against Pathogenic Bacteria

Author

Marie Champion, Emilie Portier, Karine Vallée-Réhel, Isabelle Linossier, Eric Balnois, Guillaume Vignaud, Xavier Moppert, Claire Hellio, Fabienne Faÿ, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche Dupuy de Lôme (IRDL), École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Centre National de la Recherche Scientifique (CNRS), Pacific Biotech, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Région Bretagne, and Université de Bretagne Sud

Subjects
anti-adhesive activity, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Pharmaceutical Science, Vibrio harveyi, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, anti-biofilm activity, marine bacteria, exopolysaccharide, non biocidal agent, Pseudomonas aeruginosa, Drug Discovery, exopolysaccharide anti-adhesive activity anti-biofilm activity marine bacteria non biocidal agent Pseudomonas aeruginosa Vibrio harveyi, Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Abstract

International audience; Biofilms, responsible for many serious drawbacks in the medical and marine environment, can grow on abiotic and biotic surfaces. Commercial anti-biofilm solutions, based on the use of biocides, are available but their use increases the risk of antibiotic resistance and environmental pollution in marine industries. There is an urgent need to work on the development of ecofriendly solutions, formulated without biocidal agents, that rely on the anti-adhesive physico-chemical properties of their materials. In this context, exopolysaccharides (EPSs) are natural biopolymers with complex properties than may be used as anti-adhesive agents. This study is focused on the effect of the EPS MO245, a hyaluronic acid-like polysaccharide, on the growth, adhesion, biofilm maturation, and dispersion of two pathogenic model strains, Pseudomonas aeruginosa sp. PaO1 and Vibrio harveyi DSM19623. Our results demonstrated that MO245 may limit biofilm formation, with a biofilm inhibition between 20 and 50%, without any biocidal activity. Since EPSs have no significant impact on the bacterial motility and quorum sensing factors, our results indicate that physico-chemical interactions between the bacteria and the surfaces are modified due to the presence of an adsorbed EPS layer acting as a non-adsorbing layer.

Published
2022
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12. Biopolymère amphiphile pour surface antibiofilm

Author

Guennec, Alexandra Morgane, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Bretagne Sud, Karine Vallée Réhel, and Christelle Simon-Colin

Subjects
Diatoms, Bacteria, PHA, [CHIM.ORGA]Chemical Sciences/Organic chemistry, Amphiphilic copolymer, Copolymère amphiphile, Antifouling, Analyse transcriptomique, Transcriptomic analysis
Abstract

The development of biofilms causes serious problems in the marine and medical fields. Their high tolerance to commonly used chemical agents’ disinfectants, antibiotics, biocides) makes their eradication difficult. Moreover, the use of biocide molecules is widely controversial, considering their catastrophic environmental impact. Research has therefore focused on systems that, by their composition, limit biocontamination. Among them are amphiphilic systems that can be composed of a hydrophobic polydimethylsiloxane (PDMS) matrix and an amphiphilic PDMS-PEG copolymer. Despite their efficiency, these systems are questioned because of the petrochemical origin of PDMS. The objective of this thesis project is to substitute PDMS with a biopolymer, poly(hydroxyalkanoate) (PHA). A system was formulated with PHA as a hydrophobic matrix and a PHA-PEG copolymer as an amphiphilic additive. Two types of PHA were used in this study, PHBHV (short chain length) and PHAmcl (medium chain length). The formulated coatings were characterized physically, chemically and mechanically. Then their anti-adhesive, anti-biofilm and fouling-release capacities were evaluated on different microorganisms. Two opportunistic pathogenic bacteria, Staphylococcus aureus and Pseudomonas aeruginosa, a marine bacterium, Bacillus 4J6 and two benthic diatoms, Phaeodactylum tricornutum and Navicula perminuta. Finally, in order to better understand the molecular mechanisms involved in the adhesion of S. aureus, transcriptomic analyses were performed.; Le développement de biofilms pose de sérieux problèmes dans le domaine marin et médical. Leur grande tolérance vis-à-vis d’agents chimiques utilisés habituellement (désinfectants, antibiotiques, biocides) rend leur éradication difficile. De plus, l’utilisation de molécules biocides est largement controversée, considérant leur impact environnemental catastrophique. Les recherches se sont concentrées sur des systèmes qui, par leur composition, limitent la biocontamination. Parmi eux, on trouve des systèmes amphiphiles pouvant être composés d’une matrice hydrophobe de polydiméthylsiloxane (PDMS) et d’un copolymère amphiphile PDMS-PEG. Malgré leur efficacité, ces systèmes sont remis en cause du fait de l’origine pétrochimique du PDMS. L’objectif de ce projet de thèse est de substituer le PDMS par un biopolymère, le poly(hydroxy alcanoate) (PHA). Un système a été formulé avec du PHA en tant que matrice hydrophobe et un copolymère PHA-PEG en tant qu’additif amphiphile. Deux types de PHA ont été utilisés dans cette étude, le PHBHV (PHA à courtes chaines) et le PHAmcl (PHA à moyennes chaines). Les revêtements formulés ont été caractérisés physiquement, chimiquement et mécaniquement. Puis leur capacité anti-adhésive, anti-biofilm et fouling- release ont été évaluées sur différents microorganismes. Deux bactéries pathogènes opportunistes, Staphylococcus aureus et Pseudomonas aeruginosa, une bactérie marine, Bacillus 4J6 et deux diatomées benthiques, Phaeodactylum tricornutum et Navicula perminuta. Enfin, afin de mieux comprendre le mécanisme moléculaire impliqué dans l’adhésion de S.aureus, des analyses transcriptomiques ont été réalisées.

Published
2022

13. Transcription of the oprF gene of Pseudomonas aeruginosa is dependent mainly on the SigX sigma factor and is sucrose induced

Author

Marc G. J. Feuilloley, Manjeet Bains, Nicole Orange, Robert E. W. Hancock, Sylvie Chevalier, Gwendoline Gicquel, Emeline Bouffartigues, Olivier Maillot, Alain Dufour, Alexis Bazire, Julien Vieillard, Laboratoire de Microbiologie Signaux et Microenvironnement (LMSM), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), University of British Columbia (UBC), Chimie Organique et Bioorganique : Réactivité et Analyse (COBRA), Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie Organique Fine (IRCOF), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Microbiologie du Froid – Signaux et Micro-Environnement ( LMDF-SME ), Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Normandie Université ( NU ), Laboratoire de Biotechnologie et Chimie Marines ( LBCM ), Université de Bretagne Sud ( UBS ) -Université de Brest ( UBO ), Chimie Organique et Bioorganique : Reactivité et Analyse ( COBRA ), Centre National de la Recherche Scientifique ( CNRS ) -Institut de Chimie Organique Fine ( IRCOF ), Normandie Université ( NU ) -Normandie Université ( NU ) -Institut national des sciences appliquées Rouen Normandie ( INSA Rouen Normandie ), Normandie Université ( NU ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Rouen Normandie ( UNIROUEN ), Normandie Université ( NU ) -Centre National de la Recherche Scientifique ( CNRS ), Southeastern Louisiana University, Southeastern Lousiana University, UR DER Développement et économie rurale, Institut Supérieur d'Agriculture Rhône-Alpes, Laboratoire Interdisciplinaire Carnot de Bourgogne ( LICB ), and Centre National de la Recherche Scientifique ( CNRS ) -Université de Bourgogne ( UB )

Subjects
Transcriptional Activation, Sucrose, MESH: Sodium Chloride, Transcription, Genetic, Mutant, Sigma Factor, Sodium Chloride, Biology, Microbiology, 03 medical and health sciences, Bacterial Proteins, Transcription (biology), Sigma factor, [ SDV.MP ] Life Sciences [q-bio]/Microbiology and Parasitology, MESH: Promoter Regions, Genetic, Promoter Regions, Genetic, Molecular Biology, Gene, MESH: Bacterial Proteins, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, MESH: Gene Expression Regulation, Bacterial, 030306 microbiology, MESH: Transcription, Genetic, MESH: Sucrose, MESH: Sigma Factor, Promoter, Gene Expression Regulation, Bacterial, Articles, Molecular biology, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Culture Media, MESH: Gene Deletion, Pseudomonas aeruginosa, Porin, MESH: Pseudomonas aeruginosa, MESH: Culture Media, MESH: Transcriptional Activation, [ CHIM.ANAL ] Chemical Sciences/Analytical chemistry, Cell envelope, Bacterial outer membrane, Gene Deletion
Abstract

The OprF porin is the major outer membrane protein of Pseudomonas aeruginosa . OprF is involved in several crucial functions, including cell structure, outer membrane permeability, environmental sensing, and virulence. The oprF gene is preceded by the sigX gene, which encodes the poorly studied extracytoplasmic function (ECF) sigma factor SigX. Three oprF promoters were previously identified. Two intertwined promoters dependent on σ 70 and SigX are located in the sigX - oprF intergenic region, whereas a promoter dependent on the ECF AlgU lies within the sigX gene. An additional promoter was found in the cmpX - sigX intergenic region. In this study, we dissected the contribution of each promoter region and of each sigma factor to oprF transcription using transcriptional fusions. In Luria-Bertani (LB) medium, the oprF -proximal region ( sigX - oprF intergenic region) accounted for about 80% of the oprF transcription, whereas the AlgU-dependent promoter had marginal activity. Using the sigX mutant PAOSX, we observed that the SigX-dependent promoter was largely predominant over the σ 70 -dependent promoter. oprF transcription was increased in response to low NaCl or high sucrose concentrations, and this induced transcription was strongly impaired in the absence of SigX. The lack of OprF itself increased oprF transcription. Since these conditions led to cell wall alterations, oprF transcription could be activated by signals triggered by perturbation of the cell envelope.

Published
2012
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14. Heavy Metals Concentration in Sediments of South Brittany Waters, France: An Ecological Risk Assessment Approach

Author

N.A.M. Shazili, Alexandra Connell, Virginie Dupont, David Menier, Hui-Juan Pan, M. C. Ong, Sidonie Révillon, Malaysia Institute of Oceanography, University of Malaysia, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Géosciences Océan (LGO), Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), UMR 5805 Environnements et Paléoenvironnements Océaniques et Continentaux (EPOC), Observatoire aquitain des sciences de l'univers (OASU), Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Université de Bretagne Sud (UBS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), Environnements et Paléoenvironnements OCéaniques (EPOC), and Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-École Pratique des Hautes Études (EPHE)

Subjects
Bay of Quiberon, Range (biology), Sediment, Heavy metals, Gulf of Morbihan, Heavy Metals, 6. Clean water, Current (stream), Sediment Quality Guidelines, 13. Climate action, Threshold effect, Environmental chemistry, [SDE]Environmental Sciences, Environmental science, Mariculture, Ecological risk, 14. Life underwater, Bay
Abstract

International audience; Distribution patterns of selected heavy metals content in sediments from the Bay of Quiberon and Gulf of Morbihan were studied to understand the current heavy metals contamination due to urbanization and mariculture activities in the coastal area. Therefore, a survey was conducted and 196 sediments collected were characterized for heavy metals content using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) after mix acid digestion process. The distribution maps of the concentrations of the heavy metals studied were produced as an isopleth map based on data interpolation by the ArcGIS software application. The association with the adverse effects on aquatic organisms was determined by the classification of the sediment according to the sediment quality guidelines. Therefore, two approaches were employed namely: direct comparison with Sediment Quality Guidelines (SQGs) by USEPA (United States Environmental Protection Agency) and comparison with other numerical SQGs, threshold effect level/probable effect level, and effect range low/effect range medium. In order to estimate the effect of multiple contaminations of heavy metals, the mean-ERM-quotient was calculated at each sampling point.

Published
2021
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15. A high sensitive microwave sensor to monitor bacterial and biofilm growth

Author

Longo, Matthieu, Rioual, Stéphane, Talbot, Philippe, Faÿ, Fabienne, Hellio, Claire, Lescop, Benoit, Equipe Smart Materials and Related Technologies (Lab-STICC_SMART), Laboratoire des sciences et techniques de l'information, de la communication et de la connaissance (Lab-STICC), École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-IMT Atlantique (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Institut Mines-Télécom [Paris] (IMT)-Centre National de la Recherche Scientifique (CNRS)-Université Bretagne Loire (UBL)-IMT Atlantique (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Brest (UBO), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Conductivity, Bacteria, High frequency, Monitoring, Biofilm, Signal Processing, [PHYS.COND.CM-MS]Physics [physics]/Condensed Matter [cond-mat]/Materials Science [cond-mat.mtrl-sci], Electrical and Electronic Engineering, ComputingMilieux_MISCELLANEOUS, Electronic, Optical and Magnetic Materials, Biotechnology, Sensor
Abstract

Bacterial biofilms have a significant economic and health impact in many different domains. In such films, the extracellular matrix prevents the diffusion of biocides, so antibiotic treatments require a concentration 500 to 1000 times higher than that used to eliminate the same bacteria when present as planktonic stage. Early detection of biofilms is therefore essential for effective eradication. In this paper, we present the development of a real-time and label-free radiofrequency biosensor dedicated to the monitoring of bacteria and biofilm growth. Its principle relies on an open-ended coaxial probe sensitive to the variation of the electrical conductivity of the probed medium in the microwave range. As shown, between 0.3 and 1 GHz, the high sensitivity of the method (2.3 × 104 CFU/mL) highlights the biofilm growing at the early stage of its formation. To demonstrate experimentally such effects, two model bacteria, Vibrio natriegens and P. aeruginosa, are considered. The proposed method should therefore be considered as a promising technique for biofilm monitoring in batch bioreactors or flow cells experiments.

Published
2022
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16. Antibiofilm activity in the culture supernatant of a marine Pseudomonas sp. bacterium

Author

Marianne Graber, Ana Caroline Ferro, Alexis Bazire, Florence Brian-Jaisson, Marie-Noëlle Bellon-Fontaine, Valérie Sopena, Isabelle Lanneluc, Sophie Sablé, Ibtissem Doghri, Alain Dufour, Jean-Marie Herry, LIttoral ENvironnement et Sociétés - UMR 7266 (LIENSs), Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD), MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), and AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
0303 health sciences, biology, 030306 microbiology, Chemistry, Pseudomonas aeruginosa, Pseudomonas, Biofilm, Pathogenic bacteria, bacterium, biology.organism_classification, medicine.disease_cause, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Microbiology, Bacterial cell structure, 03 medical and health sciences, Marine bacteriophage, Antibiofilm activity, medicine, 14. Life underwater, Bacteria, Flavobacterium, 030304 developmental biology
Abstract

In the marine environment, most solid surfaces are covered by microbial biofilms, mainly composed of bacteria and diatoms. The negative effects of biofilms on materials and equipment are numerous and pose a major problem for industry and human activities. Since marine micro-organisms are an important source of bioactive metabolites, it is possible that they synthesize natural ecofriendly molecules that inhibit the adhesion of organisms. In this work, the antibiofilm potential of marine bacteria was investigated using Flavobacterium sp. II2003 as a target. This strain is potentially a pioneer strain of bacteria that was previously selected from marine biofilms for its strong biofilm-forming ability. The culture supernatants of 86 marine heterotrophic bacteria were tested for their ability to inhibit Flavobacterium sp. II2003 biofilm formation and the Pseudomonas sp. IV2006 strain was identified as producing a strong antibiofilm activity. The Pseudomonas sp. IV2006 culture supernatant (SNIV2006) inhibited Flavobacterium sp. II2003 adhesion without killing the bacteria or inhibiting its growth. Moreover, SNIV2006 had no effect on the Flavobacterium sp. II2003 cell surface hydrophilic/hydrophobic and general Lewis acid–base characteristics, but modified the surface properties of glass, making it on the whole more hydrophilic and more alkaline and significantly reducing bacterial cell adhesion. The glass-coating molecules produced by Pseudomonas sp. IV2006 were found to probably be polysaccharides, whereas the antibiofilm molecules contained in SNIV2006 and acting during the 2 h adhesion step on glass and polystyrene surfaces would be proteinaceous. Finally, SNIV2006 exhibited a broad spectrum of antibiofilm activity on other marine bacteria such as Flavobacterium species that are pathogenic for fish, and human pathogens in both the medical environment, such as Staphylococcus aureus and Pseudomonas aeruginosa , and in the food industry, such as Yersinia enterocolitica . Thus, a wide range of applications could be envisaged for the SNIV2006 compounds, both in aquaculture and human health.

Published
2020
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17. Impact of co-culture on the metabolism of marine microorganisms

Author

Flore Caudal, Nathalie TAPISSIER-BONTEMPS, RuAngelie Edrada-Ebel, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Centre de recherches insulaires et observatoire de l'environnement (CRIOBE), Université de Perpignan Via Domitia (UPVD)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Excellence CORAIL (LabEX CORAIL), Institut de Recherche pour le Développement (IRD)-Université des Antilles et de la Guyane (UAG)-École des hautes études en sciences sociales (EHESS)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de La Réunion (UR)-Université de la Polynésie Française (UPF)-Université de la Nouvelle-Calédonie (UNC)-Institut d'écologie et environnement-Université des Antilles (UA), Strathclyde Institute of Pharmacy and Biomedical Sciences, and University of Strathclyde [Glasgow]

Subjects
Aquatic Organisms, Biological Products, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, natural products, marine fungi, Pharmaceutical Science, Complex Mixtures, Coculture Techniques, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, marine bacteria, Multigene Family, Drug Discovery, Fermentation, Animals, Humans, QD, Co-culture, Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Abstract

International audience; Natural products from plants have been listed for hundreds of years as a source of biologically active molecules. In recent years, the marine environment has demonstrated its ability to provide new structural entities. More than 70% of our planet’s surface is covered by oceans, and with the technical advances in diving and remotely operated vehicles, it is becoming easier to collect samples. Although the risk of rediscovery is significant, the discovery of silent gene clusters and innovative analytical techniques has renewed interest in natural product research. Different strategies have been proposed to activate these silent genes, including co-culture, or mixed fermentation, a cultivation-based approach. This review highlights the potential of co-culture of marine microorganisms to induce the production of new metabolites as well as to increase the yields of respective target metabolites with pharmacological potential, and moreover to indirectly improve the biological activity of a crude extract.

Published
2022
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18. Enzyme-assisted extraction of red seaweed Solieria chordalis (C.Agardh) J. Agardh 1842—the starting point for the production of biostimulants of plant growth and biosorbents of metal ions

Author

Olivia Spain, Kevin Hardouin, Nathalie Bourgougnon, Izabela Michalak, Laboratoire de Biotechnologie et Chimie Marine (LBCM), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Renewable Energy, Sustainability and the Environment, [SDV]Life Sciences [q-bio]
Abstract

The underexploited biomass of red seaweed Solieria chordalis (C.Agardh) J.Agardh 1842 represents a potential source for biotechnological development. Extracts obtained from S. chordalis by enzyme-assisted extraction using Protamex® and Neutrase® were evaluated as potential biostimulants of plant growth. Additionally, this alga, as well as post-extraction residues, were examined as biosorbents of metal ions. The biostimulant properties of different concentrations of algal extracts (20, 40, 60, 80, 100%) were tested in vitro on radish seeds. Chlorophyll content, plant weight, and height were measured for each experimental group. For all tested concentrations, the plants demonstrated higher chlorophyll content and were higher and heavier than the control group, showing that enzyme extracts could be used as efficient biostimulants. The biosorption properties of seaweed and post-extraction residues were tested on Cr(III) ions for different experimental conditions—pH, initial metal ion concentration, and biosorbent dosage. The maximum biosorption capacity of S. chordalis was 48.1 mg/g, for the post-extraction residue obtained with Protamex®, 47.6 mg/g, and Neutrase®, 50.5 mg/g. The binding of Cr(III) ions to the surface of biosorbents was confirmed by FT-IR analysis. Good biosorption properties of tested materials can be used in the production of novel components of fertilizers or biosorbents for wastewater treatment. In this paper, it was shown that waste biomass of S. chordalis can be turned into valuable bio-products using environmental-friendly technologies.

Published
2022
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19. Sulfated Polysaccharides from Seaweed Strandings as Renewable Source for Potential Antivirals against Herpes simplex Virus 1

Author

Hugo Pliego-Cortés, Kévin Hardouin, Gilles Bedoux, Christel Marty, Stéphane Cérantola, Yolanda Freile-Pelegrín, Daniel Robledo, Nathalie Bourgougnon, Laboratoire de Biotechnologie et Chimie Marine (LBCM), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biotechnologie et Chimie Marines, Université européenne de Bretagne - European University of Brittany (UEB)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDV]Life Sciences [q-bio], Drug Discovery, Pharmaceutical Science, Ulva sp, Sargassum muticum, Halymenia floresii, Solieria chordalis, red seaweed, Vero cells, Herpes simplex virus, polysaccharides, Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
Abstract

International audience; Herpes simplex virus 1 (HSV-1) remains a prominent health concern widespread all over the world. The increasing genital infections by HSV-1 that might facilitate acquisition and transmission of HIV-1, the cumulative evidence that HSV-1 promotes neurodegenerative disorders, and the emergence of drug resistance signify the need for new antiviral agents. In this study, the in vitro anti-herpetic activity of sulfated polysaccharides (SPs) extracted by enzyme or hot water from seaweeds collected in France and Mexico from stranding events, were evaluated. The anti-herpetic activity evaluation of the semi-refined-polysaccharides (sr-SPs) and different ion exchange purified fractions showed a wide range of antiviral activity. Among them, the sr-SPs from the Rhodophyta Halymenia floresii showed stronger activity EC50 0.68 μg/mL with SI 1470, without cytotoxicity. Further, the antiviral activity of the sr-SPs evaluated at different treatment schemes showed a high EC50 of 0.38 μg/mL during the viral adsorption assays when the polysaccharide and the virus were added simultaneously, whilst the protection on Vero cell during the post-infection assay was effective up to 1 h. The chemical composition, FTIR and 1H NMR spectroscopic, and molecular weights of the sr-SPs from H. floresii were determined and discussed based on the anti-herpetic activity. The potential utilization of seaweed stranding as a source of antiviral compounds is addressed.

Published
2022
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20. Microbiote des Echinodermes : spécificité et plasticité des microbiotes chez Holothuria forskali (Echinodermata, Holothuroidea)

Author

Laguerre, Hélène, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Bretagne occidentale - Brest, Yannick Fleury, and Patrick Le Chevalier

Subjects
Culturable microbiota, Microbiote, 16S metabarcoding, Metabarcoding 16S, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Holothuria forskali, Microbiota, Aquaculture, Échinodermes, Microbiote cultivable, Echinoderms
Abstract

Microbiota plays an essential role in the health and the developement of the host, from its earliest stages of life. With the development of next generation sequencing technologies, understanding the complex host-microbiota-environment interactions has become possible ; especially in marine invertebrates. Among them, the echinoderms constitute an exclusively marine taxonomic group and particularly interesting by their phylogenetic position.The main objective of this thesis focused on the characterization of the variations in the microbiota of Holothuria forskali (Echinodermata, Holothuroidea) by 16S metabarcoding, in multi-scale analyses.The existence of a specific microbiota associated with an echinoderm host, an anatomical compartment and a life stage has been demonstrated.Moreover, these microbiota vary according to environmental parameters, in particular with the ecological niche, but also according to the physiological state of the host.In parallel to this work on metagenomics, the cultivable microbita was also monitored and strains were isolated in order to constitute a strain library for aquaculture. Among these strains, 142 antibacterial strains were identified on the basis of their 16S rDNA and were mainly affiliated with the genera Pseudoalteromonas, Shewanella and Vibrio, very abundant in marine microbiomes.Thus, this thesis work highlighted the specificity and plasticity of the microbiota in the host H. forskali, but also the existence of a residential core microbiota composed by ubiquitous marine bacterial genera, probably playing an essential role in holobiont homeostasis.; Le microbiote joue un rôle essentiel dans la santé et le développement de l’hôte, depuis ses premiers stades de vie. Avec le développement des techniques de séquençage de nouvelle génération, la compréhension des interactions complexes hôte - microbiote - environnement est devenue possible, notamment chez les invertébrés marins. Parmi ceux-ci, les échinodermes constituent un groupe taxonomique exclusivement marin et particulièrement intéressant par leur position phylogénétique. Parmi ceux-ci, les échinodermes constituent un groupe taxonimique exclusivement marin et particulièrement intéressant par leur position phylogénétique. L’objectif principal de cette thèse a porté sur la caractérisation des variations du microbiote de Holothuria forskali (Echinodermata, Holothuroidea) par metabarocding 16S, et ce, à plusieurs échelles. L’existence d’un microbiote spécifique associé à un hôte échinoderme, à un compartiment anatomique et à un stade de vie a été démontrée. Par ailleurs, ces microbiotes varient en fonction des paramètres de l’environnement, notamment de la niche écologique, mais aussi en fonction de l’état physiologique de l’hôte. En parallèle de ces travaux réalisés en métagénomique, le microbiote cultivable a été suivi et des souches ont été isolées afin de constituer une souchothèque d’intérêt pour l’aquaculture. Parmi ces souches, 142 souches antibactériennes ont été identifiées sur la base de leur ADNr 16S et sont affiliées majoritairement aux genres Pseudoalteromonas, Shewanella et Vibrio, très abondants dans les microbiomes marins.Ainsi, les travaux de thèse ont mis en exergue la spécificité et la plasticité des microbiotes chez l’hôte H. forskali, mais également l’existence d’un core microbiote résidentiel composé de genres bactériens marins ubiquistes, jouant très probablement un rôle essentiel dans l’homéostasie de l’holobionte.

Published
2021

21. Pseudoalteromonas ostreae sp. nov., a new bacterial species harboured by the flat oyster Ostrea edulis

Author

Amine M. Boukerb, Olivier Lesouhaitier, Yannick Fleury, Leila Parizadeh, Héléna Cuny, Camille Jégou, Clément Offret, Alexis Bazire, Patrick Le Chevalier, Benjamin Brillet, Laboratoire de Biotechnologie et Chimie Marine (LBCM), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, 0303 health sciences, Oyster, biology, Strain (chemistry), [SDV]Life Sciences [q-bio], Context (language use), General Medicine, Phenotypic trait, biology.organism_classification, 16S ribosomal RNA, 010603 evolutionary biology, 01 natural sciences, Microbiology, 03 medical and health sciences, Pseudoalteromonas, biology.animal, Ostrea edulis, Ecology, Evolution, Behavior and Systematics, Bacteria, 030304 developmental biology
Abstract

Three bacterial strains, named hOe-66T, hOe-124 and hOe-125, were isolated from the haemolymph of different specimens of the flat oyster Ostrea edulis collected in Concarneau bay (Finistère, France). These strains were characterized by a polyphasic approach, including (i) whole genome analyses with 16S rRNA gene sequence alignment and pangenome analysis, determination of the G+C content, average nucleotide identity (ANI), and in silico DNA–DNA hybridization (isDDH), and (ii) fatty acid methyl ester and other phenotypic analyses. Strains hOe-66T, hOe-124 and hOe-125 were closely related to both type strains Pseudoalteromonas rhizosphaerae RA15T and Pseudoalteromonas neustonica PAMC 28425T with less than 93.3% ANI and 52.3% isDDH values. Regarding their phenotypic traits, the three strains were Gram-negative, 1–2 µm rod-shaped, aerobic, motile and non-spore-forming bacteria. Cells grew optimally at 25 °C in 2.5% NaCl and at 7–8 pH. The most abundant fatty acids were summed feature 3 (C16:1 ω7c/C16:1 ω6c), C16:0 and C17:1 ω8c. The strains carried a genome average size of 4.64 Mb and a G+C content of 40.28 mol%. The genetic and phenotypic results suggested that strains hOe-66T, hOe-124 and hOe-125 belong to a new species of the genus Pseudoalteromonas . In this context, we propose the name Pseudoalteromonas ostreae sp. nov. The type strain is hOe-66T (=CECT 30303T=CIP 111911T).

Published
2021
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22. Mathematical Modeling of Air Impingement Drying of the Brown Algae Sargassum muticum (Fucales)

Author

Pascal Morançais, Virginie Boy, Jean-Louis Lanoisellé, Jeanne Le Loeuff, Nathalie Bourgougnon, Thibaut Colinart, Laboratoire de Biotechnologie et Chimie Marine (LBCM), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, biology, Chemistry, General Chemical Engineering, [SDV]Life Sciences [q-bio], 04 agricultural and veterinary sciences, General Chemistry, biology.organism_classification, 040401 food science, 01 natural sciences, Industrial and Manufacturing Engineering, Brown algae, 0404 agricultural biotechnology, 010608 biotechnology, Environmental chemistry, Air drying, Sorption isotherm, Sargassum muticum, Fucales, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2021
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23. Inter-Kingdom Signaling of Stress Hormones: Sensing, Transport and Modulation of Bacterial Physiology

Author

Mohamed Zommiti, Rikki Knowlton, Ouiza Mesguida, Mélyssa Cambronel, Marc G. J. Feuilloley, Sophie Rodrigues, Nathalie Connil, Amine M. Boukerb, Laboratoire de Biotechnologie et Chimie Marine (LBCM), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Microbiology (medical), [SDV]Life Sciences [q-bio], Virulence, Physiology, Review, medicine.disease_cause, Microbiology, 03 medical and health sciences, Immune system, stress hormones, medicine, Escherichia coli, sensing, 030304 developmental biology, 0303 health sciences, biology, 030306 microbiology, Biofilm, biology.organism_classification, Vibrio, QR1-502, bacterial physiology, Salmonella enterica, transport, catecholamines, Bacteria, Hormone
Abstract

Prokaryotes and eukaryotes have coexisted for millions of years. The hormonal communication between microorganisms and their hosts, dubbed inter-kingdom signaling, is a recent field of research. Eukaryotic signals such as hormones, neurotransmitters or immune system molecules have been shown to modulate bacterial physiology. Among them, catecholamines hormones epinephrine/norepinephrine, released during stress and physical effort, or used therapeutically as inotropes have been described to affect bacterial behaviors (i.e., motility, biofilm formation, virulence) of various Gram-negative bacteria (e.g., Escherichia coli, Salmonella enterica serovar Typhimurium, Pseudomonas aeruginosa, Vibrio sp.). More recently, these molecules were also shown to influence the physiology of some Gram-positive bacteria like Enterococcus faecalis. In E. coli and S. enterica, the stress-associated mammalian hormones epinephrine and norepinephrine trigger a signaling cascade by interacting with the QseC histidine sensor kinase protein. No catecholamine sensors have been well described yet in other bacteria. This review aims to provide an up to date report on catecholamine sensors in eukaryotes and prokaryotes, their transport, and known effects on bacteria.

Published
2021
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24. Antifouling properties of amphiphilic poly(3-hydroxyalkanoate): an environmentally-friendly coating

Author

Isabelle Linossier, A Guennec, Estelle Renard, Valérie Langlois, E Balnois, Fabienne Faÿ, G Q Chen, Karine Vallée-Réhel, L Brelle, Christelle Simon-Colin, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biotechnologie et Chimie Marine (LBCM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Chimie et des Matériaux Paris-Est (ICMPE), Institut de Chimie du CNRS (INC)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), and Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
Materials science, Biofouling, Polymers, Surface Properties, 02 engineering and technology, Aquatic Science, engineering.material, 010402 general chemistry, 01 natural sciences, Applied Microbiology and Biotechnology, Polyethylene Glycols, chemistry.chemical_compound, Coating, Amphiphile, Copolymer, [CHIM]Chemical Sciences, Phaeodactylum tricornutum, ComputingMilieux_MISCELLANEOUS, Water Science and Technology, Diatoms, biology, Polydimethylsiloxane, 021001 nanoscience & nanotechnology, biology.organism_classification, Environmentally friendly, 6. Clean water, 0104 chemical sciences, chemistry, Chemical engineering, engineering, Biopolymer, 0210 nano-technology
Abstract

The development of biofouling is a major problem for marine industries. The conception of antifouling and fouling release coatings, with controlled physical-chemical properties is a promising strategy. Among them, amphiphilic systems, such as those composed of a hydrophobic polydimethylsiloxane matrix and a hydrophilic polyethyleneglycol additive are the most efficient and up to date. Despite their effectiveness, these systems are questioned due to the petrochemical origin of PDMS. The aim of this project was to substitute the PDMS matrix with a biopolymer, poly(3-hydroxybuyrate-co-3-hydroxyvalerate) and to improve its anti-adhesion properties through the elaboration of an amphiphilic system, via the addition of PEG or PHBHHx-b-PEG copolymer. The results, including the physico-chemical properties of PHBHV based coatings and static adhesion tests on a marine bacterium, Bacillus 4J6 and a diatom, Phaeodactylum tricornutum are compared with those of PDMS and PEG-modified PDMS coatings. Real antiadhesion activity was obtained for the PHBHV/PHBHHx-b-PEG system for a promising eco-friendly strategy.

Published
2021
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25. Virulence of Vibrio harveyi ORM4 towards the European abalone Haliotis tuberculata involves both quorum sensing and a type III secretion system

Author

Morot, Amandine, El Fekih, Sahar, Bidault, Adeline, Le Ferrand, Alizée, Jouault, Albane, Kavousi, Javid, Bazire, Alexis, Pichereau, Vianney, Dufour, Alain, Paillard, Christine, Delavat, François, Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), This work was supported by the ‘Laboratoire d'Excellence’ LabexMER (ANR-10-LABX-19), by the ‘Ecole Universitaire de Recherche’ ISblue (ANR-17-EURE-0015) and co-funded by a grant from the French government under the programme ‘Investissements d'Avenir’, and by a grant from the Regional Council of Brittany. This project received grants from the H2020 VIVALDI project (grant agreement n°678589), and the CNRS Prematuration (CoMAq project) and EC2CO (ViViMar project) programmes. The LBCM is supported by the Région Bretagne and European FEDER. A.M. and A.J. are the recipient of doctoral fellowships co-funded by the Région Bretagne, France, and the Université de Bretagne-Sud., ANR-17-EURE-0015,ISBlue,Interdisciplinary Graduate School for the Blue planet(2017), ANR-10-LABX-0019,LabexMER,LabexMER Marine Excellence Research: a changing ocean(2010), European Project: 678589,H2020,H2020-SFS-2015-2,VIVALDI(2016), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
[SDE]Environmental Sciences, bacteria, ComputingMilieux_MISCELLANEOUS
Abstract

International audience; Environmental Vibrio strains represent a major threat in aquaculture, but the understanding of their virulence mechanisms heavily relies on the transposition of knowledge from human-pathogen vibrios. Here, the genetic bases of the virulence of Vibrio harveyi ORM4 towards the European abalone Haliotis tuberculata were characterized. We demonstrated that luxO, encoding a major regulator of the quorum sensing system, is crucial for the virulence of this strain, and that its deletion leads to a decrease in swimming motility, biofilm formation, and exopolysaccharide production. Furthermore, the biofilm formation by V. harveyi ORM4 was increased by abalone serum, which required LuxO. The absence of LuxO in V. harveyi ORM4 yielded opposite phenotypes compared with other Vibrio species including V. campbellii (still frequently named V. harveyi). In addition, we report a full type III secretion system (T3SS) gene cluster in the V. harveyi ORM4 genome. LuxO was shown to negatively regulate the promoter activity of exsA, encoding the major regulator of the T3SS genes, and the deletion of exsA abolished the virulence of V. harveyi ORM4. These results unveil virulence mechanisms set up by this environmentally important bacterial pathogen and pave the way for a better molecular understanding of the regulation of its pathogenicity.

Published
2021
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26. Cinnamomum cassia and Syzygium aromaticum Essential Oils Reduce the Colonization of Salmonella Typhimurium in an In Vivo Infection Model Using Caenorhabditis elegans

Author

Emmanuel Duteil, Marie Lang, Aude Montjarret, Gilles Bedoux, Laboratoire de Biotechnologie et Chimie Marine (LBCM), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Salmonella, [SDV]Life Sciences [q-bio], Pharmaceutical Science, Organic chemistry, medicine.disease_cause, Caenorhabditis elegans, swimming motility, Article, Analytical Chemistry, 03 medical and health sciences, QD241-441, Cassia, In vivo, Drug Discovery, medicine, Food science, Physical and Theoretical Chemistry, essential oils, 030304 developmental biology, 0303 health sciences, feed additives, Salmonella Typhimurium, biology, colonization assay, 030306 microbiology, Chemistry, biology.organism_classification, Nematode, Chemistry (miscellaneous), Syzygium, Toxicity, Molecular Medicine, Cinnamomum
Abstract

The regulation of intestinal colonization in livestock by means of non-bactericidal additives is an important management lever for zoonotic bacteria such as Salmonella spp. Caenorhabditis elegans is proposed here as a model for the evaluation of five essential oils (EOs) as anti-colonization products against Salmonella Typhimurium. An evaluation of the toxicity of EOs for C. elegans showed LD50 values ranging from 74.5 ± 9.6 µg/mL for Cinnamomum cassia (CEO) to 271.6 ± 14.9 µg/mL for Syzygium aromaticum (SyEO). Both EOs significantly inhibited bacterial colonization in the digestive tract of C. elegans with reductions of 0.88 and 0.70 log CFU/nematode at nontoxic concentrations of 50 µg/mL and 150 µg/mL, respectively. With the minimal bactericidal concentrations of CEO and SyEO against S. Typhimurium being 312.5 µg/mL and 625 µg/mL, respectively, an antibacterial effect can be excluded to explain the inhibition of the bacterial load. The anti-colonizing activity of these two EOs could, however, be related to an inhibition of the swimming motility, which was significantly reduced by 23.47% for CEO at 50 µg/mL and 19.56% for SyEO at 150 µg/mL. This study shows the potential of C. elegans as a predictive in vivo model of anti-colonizing activities that is suitable for the evaluation of essential oils.

Published
2021
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27. Investigations by AFM of Ageing Mechanisms in PLA-Flax Fibre Composites during Garden Composting

Author

Delphin Pantaloni, Eric Balnois, Christophe Baley, Alessia Melelli, Olivier Arnould, Frédéric Jamme, Darshil U. Shah, Alain Bourmaud, Johnny Beaugrand, Shah, Darshil [0000-0002-8078-6802], Apollo - University of Cambridge Repository, Melelli, Alessia [0000-0003-0618-0041], Arnould, Olivier [0000-0001-8328-0359], Jamme, Frédéric [0000-0002-7398-7868], Shah, Darshil U [0000-0002-8078-6802], Bourmaud, Alain [0000-0003-4584-4242], Shah, Darshil U. [0000-0002-8078-6802], Institut de Recherche Dupuy de Lôme (IRDL), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Bois (BOIS), Laboratoire de Mécanique et Génie Civil (LMGC), Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Synchrotron SOLEIL (SSOLEIL), Centre National de la Recherche Scientifique (CNRS), Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of Cambridge [UK] (CAM), INTERREG IV Cross Channel programme through the FLOWER project : 23., and École Nationale d'Ingénieurs de Brest (ENIB)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-École Nationale Supérieure de Techniques Avancées Bretagne (ENSTA Bretagne)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Materials science, Morphology (linguistics), Polymers and Plastics, Scanning electron microscope, Composite number, Organic chemistry, Modulus, Mechanical properties, 02 engineering and technology, Surface finish, 010402 general chemistry, 01 natural sciences, Article, QD241-441, stomatognathic system, Indentation, Composite material, Porosity, Structural Degradation, [CHIM.MATE]Chemical Sciences/Material chemistry, General Chemistry, 021001 nanoscience & nanotechnology, Flax fibre, 0104 chemical sciences, [CHIM.POLY]Chemical Sciences/Polymers, SEM, Degradation (geology), PLA, AFM, 0210 nano-technology
Abstract

International audience; PLA-flax non-woven composites are promising materials, coupling high performance and possible degradation at their end of life. To explore their ageing mechanisms during garden composting, microstructural investigations were carried out through scanning electron microscopy (SEM) and atomic force microscopy (AFM). We observe that flax fibres preferentially degrade ‘inwards’ from the edge to the core of the composite. In addition, progressive erosion of the cell walls occurs within the fibres themselves, ‘outwards’ from the central lumen to the periphery primary wall. This preferential degradation is reflected in the decrease in indentation modulus from around 23 GPa for fibres located in the preserved core of the composite to 3–4 GPa for the remaining outer-most cell wall crowns located at the edge of the sample that is in contact with the compost. Ageing of the PLA matrix is less drastic with a relatively stable indentation modulus. Nevertheless, a change in the PLA morphology, a significant decrease in its roughness and increase of porosity, can be observed towards the edge of the sample, in comparison to the core. This work highlights the important role of intrinsic fibre porosity, called lumen, which is suspected to be a major variable of the compost ageing process, providing pathways of entry for moisture and microorganisms that are involved in cell wall degradation.

Published
2021
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28. Evaluation of immunomodulatory activities of essential oils by high content analysis

Author

Emmanuel Duteil, Pierre-Jean Ferron, Marie Lang, Alexis Bazire, Aude Montjarret, Gilles Bedoux, Julian Bursztyka, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD), Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Xénobiotiques, Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Bioarmor development SARL, Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université de Toulouse (UT)-Université de Toulouse (UT)

Subjects
Lipopolysaccharides, 0106 biological sciences, 0301 basic medicine, Cinnamomum zeylanicum, Phagocytosis, Bioengineering, Biology, 01 natural sciences, Applied Microbiology and Biotechnology, law.invention, Mice, 03 medical and health sciences, Overall response rate, law, 010608 biotechnology, Oils, Volatile, Animals, Plant Oils, Food science, ComputingMilieux_MISCELLANEOUS, Essential oil, Inflammation, Dose-Response Relationship, Drug, General Medicine, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, RAW 264.7 Cells, 030104 developmental biology, Clove Oil, High-content screening, Mentha, Biotechnology
Abstract

Nowadays, research concerning immunomodulatory products are of great interest, particularly in the treatment of inflammatory diseases or the prevention of infectious diseases. These activities are usually evaluated on cell cultures, by tracking different factors requiring dedicated manipulation. Evaluation of the immunomodulatory activities of essential oils and pure compounds using several technics adapted to high content analysis is described in this study. This approach allows a multiparametric evaluation on a single cell batch, in order to obtain an overall response. The developed method is based on the simultaneous evaluation of phagocytosis, production of iNOS and secretion of IL-6, induced by contact of RAW 264.7 cells with LPS. The results highlight the immunomodulatory activities of cinnamon and clove essential oils. They also provide information, particularly concerning the inhibitory activity of mint essential oil, which inhibits the LPS-induced phagocytosis of RAW 264.7 cells by 42%, at 100 μg/ml. This work presents for the first time the adaptation of high content analyses for the monitoring of immunomodulatory activities of essential oils. This protocol could be adjustable to other cell types and supplemented by the evaluation of additional parameters.

Published
2019
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29. Étude de l'adhésion de diatomées benthiques et du potentiel antifouling de zéolites chargées en cuivre

Author

Traon, Florian, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Bretagne Sud, Isabelle Linossier, and Fabienne Faÿ

Subjects
Zeolite, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Biofouling, Adhesion, Diatom, Antifouling, [INFO.INFO-BT]Computer Science [cs]/Biotechnology
Abstract

Biofouling development on anthropic infrastuctures remain a permanent issue causing economic and environmental damages. This phenomenon created by diverse trophic leve;s generally begin with the microfouling step, mainly composed of bacteria and microalgae, and the macrofouling one represented by the settlement of macroalgae and molluscs. To fight against this fouling, the development of antifouling strategies is crucial. Recently, pollution caused by these coatings has stimulated the research for efficient and green systems. The aim of this thesis is to study the adhesion of benthic diatoms, well known to adapt to different surfaces. On another hand, the use of copper loaded zeolites is evaluated to limit the adhesion and development of fouling diatoms. Firstly, microscopic and sequencing identification of fouling diatoms allow to determine the main genus present on antifouling coatings. Secondly, the activities of zeolites, pure or copper loaded, on the microalgae Cylindrotheca closterium and Amphora sp. are evaluated. These results highlight the mode of action of zeolites on diatoms. Then, zeolites are integrated in bio-based polymer coatings to determine their efficiency to inhibit diatom adhesion. Finally, the analyses of monosaccharidic and amino acid compositions of diatom adhesives is realized following their adhesion on substrates with different surface properties.; Le développement du biofouling sur les infrastructures anthropiques est un problème permanent causant des dégâts économiques et environnementaux. Ce phénomène causé par divers niveaux trophiques commence généralement par l’étape du microfouling, constitué majoritairement de bactéries et microalgues, puis celui du macrofouling représenté par l’établissement de macroalgues et mollusques. Afin de lutter contre ces salissures, le développement de stratégies antifouling est crucial. Récemment, la pollution causée par ces revêtements a relancé la recherche pour trouver des systèmes efficaces tout en étant plus respectueux de l’environnement. L’objectif de cette thèse est d’étudier l’adhésion des diatomées benthiques, connues pour leur capacité à s’adapter à différents types de substrats. D’un autre côté, l’utilisation de zéolites chargées en cuivre pour limiter l’adhésion et le développement de microalgues modèles est évaluée. Tout d’abord, l’identification de diatomées colonisatrices, par microscopie et séquençage, permet de déterminer les principaux genres présents sur des revêtements antifouling avec biocides. Ensuite, l’étude de l’activité de zéolites, vierges et chargées en cuivre, sur les microalgues Cylindrotheca closterium et Amphora sp. est réalisée. Ces résultats mettent en avant le mode d’action des zéolites sur les diatomées benthiques. Les zéolites sont ensuite intégrées dans des revêtements à base de polymère biosourcé afin d’en évaluer l’efficacité à inhiber l’adhésion. Enfin, l’analyse des compositions monosaccharidiques et en acides aminés des adhésifs microalgaux est réalisée suite à l’adhésion des diatomées sur des substrats aux propriétés de surfaces différentes.

Published
2021

30. Participatory sciences dedicated to the sea and coast of Brittany: state of the art, recommendations and prospects for ISblue universities

Author

sauleau, pierre, Poisson, Pauline, Ruault, Riwalenn, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Muséum national d'Histoire naturelle (MNHN), Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), and IUEM Institut Universitaire Européen de la Mer

Subjects
université, mer, sciences participatives, éducation, Bretagne, [SDE.ES]Environmental Sciences/Environmental and Society
Abstract

Ocean acidification, marine biodiversity loss, chemical and biological pollutions, overexploitation of natural resources affect not only marine ecosystems and the coastline but also the cultural heritage of the Atlantic coast. These great challenges go well beyond the sole concerns of scientists who, more and more, associate with citizens, not only to collect data, but also to participate in the creation and implementation of research programs.Relationships at the interface between science, technology and citizenship are changing very rapidly in our society. With the development of digital technology, participatory sciences dedicated to the sea and the coast are multiplying. The pivotal role of a "participatory science coordinator" becomes essential for the proper functioning of these programs in order to mediate between the scientist and the citizen. In order to respond to the creation of new maritime professions and to ensure the professional integration of students, participatory sciences then become an additional science to be taught in universities.This article aims to present the challenges of the Teaching of Participatory Sciences (ESPAR) project dedicated to the marine environment and the coast, led by the University Research School (EUR) ISblue (Interdisciplinary graduate School for the blue planet). In the first part of this article, a state of the art of participatory science programs currently being carried out in the West is presented. After some recommendations for the development of participatory sciences dedicated to the marine world and the coast, various perspectives are made, particularly in terms of educational innovations at the university.; L’acidification des océans, l’érosion de la biodiversité marine, la pollution chimique et biologique, la surexploitation des ressources naturelles affectent non seulement les écosystèmes marins, le trait de côte, mais aussi le patrimoine culturel du littoral atlantique. Ces grands défis vont bien au-delà des seules préoccupations des scientifiques qui, de plus en plus, s’associent avec les citoyens, non seulement pour collecter les données, mais aussi participer à la création et à la mise en œuvre de programmes de recherche.Les relations à l’interface entre science, technologie et citoyenneté évoluent très rapidement au sein de notre société. Avec le développement du numérique, les sciences participatives dédiées à la mer et au littoral se multiplient. Le rôle pivot d’un « coordinateur en sciences participatives » devient essentiel au bon fonctionnement de ces programmes afin d’assurer une médiation entre le scientifique et le citoyen. Afin de répondre à la création de nouveaux métiers de la mer et d’assurer l’insertion professionnelle des étudiants, les sciences participatives deviennent alors une science supplémentaire à enseigner au sein des universités.Cet article vise à présenter les enjeux du projet d’Enseignement des Sciences PARticipatives (ESPAR) dédiées au milieu marin et au littoral, porté par l’Ecole Universitaire de Recherche (EUR) ISblue (Interdisciplinary graduate School for the blue planet). Dans la première partie de cet article est présenté un état de l’art des programmes de sciences participatives menés actuellement dans le grand Ouest. Après quelques recommandations pour le développement des sciences participatives dédiées au monde marin et au littoral, sont émises diverses perspectives notamment en termes d’innovations pédagogiques à l’université.

Published
2021

31. Extraction et évaluation des propriétés biologiques des ulvanes et oligo-ulvanes de l'algue verte Ulva sp. : actions sur le métabolisme de cellules cutanées et sur le microbiote cutané

Author

Fournière, Mathilde, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Bretagne Sud, Nathalie Bourgougnon, Gilles Bedoux, Thomas Latire, and STAR, ABES

Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology, Extraction assistée par enzyme, Skin microbiota, Ulvanes, [SDV.BC]Life Sciences [q-bio]/Cellular Biology, [SDV.MHEP.DERM] Life Sciences [q-bio]/Human health and pathology/Dermatology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Microbiote cutané, Ulvans, [SDV.BC] Life Sciences [q-bio]/Cellular Biology, Enzyme-assisted extraction, [SDV.MHEP.DERM]Life Sciences [q-bio]/Human health and pathology/Dermatology
Abstract

Ulvans are matrix sulfated polysaccharides constitutive of green seaweed Ulva sp. cell wall. Although proliferative and quantitatively available, Ulva sp. is little valued with applications in low added value sectors. The objective of this project is to produce fractions enriched in ulvans and oligo-ulvans in order to evaluate their biological properties on cutaneous system. Ulvan fractions were produced by maceration and enzyme-assisted extraction followed by different purification processes: ethanolic precipitation and dialysis. Radical and acid depolymerization processes allowed to obtain oligo-ulvan fractions. Ulvan and oligo-ulvan fractions have been shown to stimulate human dermal fibroblasts proliferation. An increase in the synthesis of extracellular matrix components such as type I and III collagens, and glycosaminoglycans has been demonstrated. Fractions also stimulate the expression, synthesis and MMP-1 enzymatic activity. At the skin microbiota level, the fractions do not alter the growth of commensal bacteria S. epidermidis, S. aureus and C. acnes, but may modify the biofilm formation. Ulvan and oligo-ulvan fractions also reduce the inflammatory potential of HaCaT keratinocytes induced by C. acnes. Thus, this work has shown that the ulvan and oligo-ulvan fractions exhibit promising biological activities for dermo-cosmetic applications in tissue repair, anti-ageing or anti-inflammatory strategies while preserving commensal skin microbiota., Les ulvanes sont des polysaccharides matriciels sulfatés constitutifs de la paroi de l’algue verte Ulva sp.. Bien que proliférative et quantitativement disponible, Ulva sp. est peu valorisée avec des applications dans des domaines à faible valeur ajoutée. Ce projet de thèse a pour objectif la production de fractions enrichies en ulvanes et en oligo-ulvanes dans le but d’évaluer leurs propriétés biologiques sur le système cutané. Les fractions d’ulvanes ont été produites par macération et extraction assistée par enzyme suivie de différents procédés de purification : précipitation éthanolique et dialyse. Des dépolymérisations radicalaire et acide ont permis d’obtenir des fractions d’oligo-ulvanes. Ces fractions d’ulvanes et d’oligo-ulvanes stimulent la prolifération de fibroblastes dermiques humains. Une augmentation de la synthèse protéique de composants matriciels dont les collagènes de type I et III, et les glycosaminoglycanes a été mise en évidence. Les fractions stimulent également l’expression, la synthèse et l’activité enzymatique de MMP-1. Au niveau du microbiote cutané, ces fractions n’altèrent pas la croissance des bactéries commensales S. epidermidis, S. aureus et C. acnes, mais peuvent modifier la formation de biofilm. Les fractions d’ulvanes et d’oligo-ulvanes diminuent également le potentiel inflammatoire des kératinocytes HaCaT induit par C. acnes. Ainsi, les travaux ont démontré que les fractions d’ulvanes et d’oligo-ulvanes présentent des activités biologiques prometteuses pour des applications dermo-cosmétiques dans le cadre de stratégies de réparation tissulaire, anti-vieillissement, ou anti-inflammatoire, dans le respect du microbiote commensal cutané.

Published
2021

32. Temperate and tropical coastal waters share relatively similar microbial biofilm communities while free-living or particle-attached communities are distinct

Author

Jean Turquet, Cédric Garnier, Karine Réhel, Thomas Pollet, Isabelle Linossier, Raphaëlle Barry-Martinet, Jean-François Briand, C P Elisa Catão, Alina Tunin-Ley, Laboratoire Matériaux Polymères Interfaces Environnement Marin - EA 4323 (MAPIEM), Université de Toulon (UTLN), Animal, Santé, Territoires, Risques et Ecosystèmes (UMR ASTRE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut méditerranéen d'océanologie (MIO), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Toulon (UTLN)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Cyclotron Réunion Océan Indien (CYROI), Université de La Réunion (UR)-Centre Hospitalier Universitaire de La Réunion (CHU La Réunion), Centre technique d'appui à la pêche réunionnaise (CAP RUN - CITEB) [France], and ANR-12-CDII-0008,BIOPAINTROP,Revêtements antifouling écologiques d'origine tropicale(2012)

Subjects
0106 biological sciences, 0301 basic medicine, lifestyle, Rare biosphere, [SDV]Life Sciences [q-bio], Biology, 010603 evolutionary biology, 01 natural sciences, prokaryotes, 03 medical and health sciences, Nutrient, Mediterranean sea, RNA, Ribosomal, 16S, Genetics, Temperate climate, Mediterranean Sea, Trace metal, 14. Life underwater, Indian Ocean, marine biofilms, Ecology, Evolution, Behavior and Systematics, Ecology, Microbiota, Biofilm, Plankton, 030104 developmental biology, 13. Climate action, Biofilms, environmental drivers, geographical effect, Water quality
Abstract

International audience; Free-living (FL) marine microbial communities differ from those attached to particles (PA). Likewise, biofilms (B) colonizing artificial surfaces, including plastics or ship hulls, hardly resemble their planktonic surroundings. However, few studies have examined the effect of the environment on these lifestyles and on the source of organisms colonizing marine surfaces. Using 16S rRNA gene metabarcoding, we identified specificities of marine prokaryotic community lifestyles (FL, PA or B) sampled in three coastal polluted locations with dissimilar environmental conditions: the North-Western Mediterranean Sea and the Atlantic and Indian Oceans. Biofilms developed over polyvinyl chloride (PVC) were found to be significantly different from FL or PA collected during the immersions. Alpha-diversity increased from FL to PA and to B, illustrating the integrative aspect of the latter, with little proportion of operational taxonomic units shared with the first two. Beta-diversity clustered first the lifestyles and then the sites. FL and PA were more affected by water quality, especially by trace metal contamination, whereas B were as sensitive to trace metals as to nutrients. Although biofilms should be supplied by the planktonic (ultra) rare biosphere, source tracking could only detect small contributions of FL or PA taxa to B communities.

Published
2021
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33. Poly(oxazoline) for the design of amphiphilic silicone coatings

Author

Vincent Lapinte, Belkacem Tarek Benkhaled, Émilie Portier, Karine Réhel, Jean-François Bardeau, Fabrice Azemar, Isabelle Linossier, Fabienne Faÿ, Institut des Molécules et Matériaux du Mans (IMMM), Le Mans Université (UM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), Institut Charles Gerhardt Montpellier - Institut de Chimie Moléculaire et des Matériaux de Montpellier (ICGM ICMMM), and Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut de Chimie du CNRS (INC)

Subjects
Materials science, General Chemical Engineering, Context (language use), macromolecular substances, 02 engineering and technology, Oxazoline, engineering.material, [SPI.MAT]Engineering Sciences [physics]/Materials, 03 medical and health sciences, chemistry.chemical_compound, Silicone, Coating, Amphiphile, PEG ratio, Materials Chemistry, [CHIM]Chemical Sciences, RTV silicone, ComputingMilieux_MISCELLANEOUS, 0303 health sciences, Fouling, 030306 microbiology, Organic Chemistry, technology, industry, and agriculture, 021001 nanoscience & nanotechnology, [PHYS.PHYS.PHYS-GEN-PH]Physics [physics]/Physics [physics]/General Physics [physics.gen-ph], Surfaces, Coatings and Films, chemistry, Chemical engineering, engineering, [PHYS.PHYS.PHYS-CHEM-PH]Physics [physics]/Physics [physics]/Chemical Physics [physics.chem-ph], 0210 nano-technology
Abstract

Fouling release coatings are known to be ecofriendly and to be a good alternative to the coatings containing biocides. To improve their efficiency and lifespan the incorporation of additives seems required. Amphiphilic system as PEG-silicone coatings have a lower impact on the environment. However, PEG oxidation in seawater impacts its lifespan. In this context, the development and incorporation of new hydrophilic additives offers great potentiel. The objective of this study is to design an amphiphilic fouling release coating containing poly(oxazoline) (POx) as additives. POx have similar physical properties as PEG and have already been used in biomedical applications. POx with a trimethoxysilane end-group has been synthesized to be crosslinked in a RTV silicone coating. The impact of its incorporation in a PDMS coating has been evaluated on surface properties and on coating organization. The results have been compared with a similar PEG-silicone coating. In addition, microbiologic assays have been carried out to evaluate the bacterial adhesion and the fouling properties of the coating. Two biomedicals bacteria and a marine bacterium were used to confirm the interest of POx. POx-silicone coating showed similar fouling efficiency against bacteria as PEG-silicone coating despite a bigger surface roughness and a lower compatibility with PDMS.

Published
2021
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34. Poly- and Oligosaccharide Ulva sp. Fractions from Enzyme-Assisted Extraction Modulate the Metabolism of Extracellular Matrix in Human Skin Fibroblasts: Potential in Anti-Aging Dermo-Cosmetic Applications

Author

Thomas Latire, Nicolas Lebonvallet, Raphael Leschiera, Gilles Bedoux, Claudie Le Goff-Pain, Mathilde Fournière, Nathalie Bourgougnon, Laboratoire de Biotechnologie et Chimie Marines, Université européenne de Bretagne - European University of Brittany (UEB)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biotechnologie et Chimie Marine (LBCM), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire sur les interactions Epithéliums Neurones (LIEN), and Université de Brest (UBO)

Subjects
0106 biological sciences, Proteomics, collagen, matrix metalloproteinase, [SDV]Life Sciences [q-bio], extracellular matrix, Pharmaceutical Science, Oligosaccharides, Human skin, Cosmetics, Matrix (biology), Polysaccharide, 01 natural sciences, Article, Dermal fibroblast, Extracellular matrix, Glycosaminoglycan, 03 medical and health sciences, Ulva, Polysaccharides, Drug Discovery, Humans, Pharmacology, Toxicology and Pharmaceutics (miscellaneous), lcsh:QH301-705.5, Cells, Cultured, 030304 developmental biology, Cell Proliferation, Skin, chemistry.chemical_classification, 0303 health sciences, Ulva sp, Gene Expression Profiling, Biological activity, Oligosaccharide, Fibroblasts, human dermal fibroblast, Skin Aging, chemistry, Biochemistry, lcsh:Biology (General), seaweed, Matrix Metalloproteinase 1, 010606 plant biology & botany
Abstract

Ulva sp. is known to be a source of bioactive compounds such as ulvans, but their biological activity on human dermal fibroblast extracellular matrix (ECM) is poorly reported. In this work, the regulation of ECM has been investigated for the first time at both proteomic and transcriptomic levels in normal human skin dermal fibroblasts, after 48 h of incubation with poly- and oligosaccharide fractions from Ulva sp. obtained after enzyme-assisted extraction and depolymerization. Cell proliferation enhancement (up to +68%) without exhibiting any cytotoxic effect on fibroblasts was demonstrated at 50 and 1000 µg/mL by both fractions. At the proteomic level, polysaccharide fractions at 1000 µg/mL enhanced the most the synthesis of glycosaminoglycans (GAGs, up to +57%), total collagen, especially types I (up to +217%) and III, as well as the synthesis and activity of MMP-1 (Matrix Metalloproteinase-1, up to +309%). In contrast, oligosaccharide fractions had no effect on GAGs synthesis but exhibited similarities for collagens and MMP-1 regulation. At the transcriptomic level, the decrease of COL1A1 and COL1A2 expression, and increase of COL3A1 and MMP-1 expression, confirmed the modulation of ECM metabolism by both fractions. Our research emphasizes that poly- and oligosaccharide Ulva sp. fractions exhibit interesting biological activities and supports their potential use in the area of skin renewal for anti-aging dermo-cosmetic applications.

Published
2021
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35. Algal Derived Functional Lipids and their Role in Promoting Health

Author

Mathilde Fournière, Nolwenn Terme, Benoît Chénais, Gilles Bedoux, Nathalie Bourgougnon, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Mer, molécules et santé EA 2160 (MMS), Le Mans Université (UM)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), Université de Nantes (UN)-Université de Nantes (UN)-Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques, Université de Nantes (UN)-Université de Nantes (UN), Gaurav Rajauria, Yvonne V. Yuan, Université de Nantes - UFR des Sciences Pharmaceutiques et Biologiques, and Université de Nantes (UN)-Université de Nantes (UN)-Le Mans Université (UM)-Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST)

Subjects
macroalgae, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, purification, media_common.quotation_subject, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, Biology, human health, Cosmetics, fatty acids, metabolic syndrome, 03 medical and health sciences, Human health, 0302 clinical medicine, cardiovascular disease, medicine, cancer, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, cosmetic, 030304 developmental biology, media_common, 0303 health sciences, Traditional medicine, microalgae, Cancer, medicine.disease, 3. Good health, 13. Climate action, 030220 oncology & carcinogenesis, seaweed, Metabolic syndrome, [CHIM.OTHE]Chemical Sciences/Other, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

International audience; Algae are organisms with an enormous biodiversity and contain manifold fatty acid molecular structures and ratios. This chapter presents information about the structure and distribution of fatty acids in algae whether microalgae or macroalgae. The parameters affecting lipid and fatty acid contents as well as distribution are also discussed. Methods for isolation and purification of fatty acids from algae to obtain highly pure fatty acid or fatty acid fractions with good overall yields are presented. Algal fatty acids are known as sources of bioactive compounds for health promotion and wellness. Lipid fractions and fatty acids act as actives or additives for cosmeceutical applications. For health promotion, preventive effects of n-3 polyunsaturated fatty acids on cardiovascular disease, metabolic syndrome and cancer are discussed. The role of n-3 fatty acids in cancer therapy and antiviral activities of lipids are also exposed. Furthermore, potential commercial application domains and concepts are discussed in this chapter.

Published
2021
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36. Anti-Herpes simplex virus (HSV-1) activity and antioxidant capacity of carrageenan-rich enzymatic extracts from Solieria filiformis (Gigartinales, Rhodophyta)

Author

Madera-Santana Tomás, Bedoux Gilles, Bourgougnon Nathalie, Peñuela Ana, Freile-Pelegrin Yolanda, Robledo Daniel, Laboratoire de Biotechnologie et Chimie Marine (LBCM), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Antioxidant, medicine.medical_treatment, [SDV]Life Sciences [q-bio], Carbohydrase, Herpesvirus 1, Human, 02 engineering and technology, Carrageenan, Polysaccharide, Antiviral Agents, Biochemistry, Antioxidants, Hydrolysate, 03 medical and health sciences, chemistry.chemical_compound, Phenols, Picrates, Polysaccharides, Structural Biology, medicine, Molecular Biology, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, ABTS, Protease, biology, Plant Extracts, Biphenyl Compounds, General Medicine, 021001 nanoscience & nanotechnology, chemistry, Rhodophyta, biology.protein, 0210 nano-technology
Abstract

Solieria filiformis has been reported to have molecules with various biological activities. In this study we used environmentally friendly extraction methods, such as enzyme-assisted extraction (EAE), as a first step to obtain bioactive compounds from this species. Five combinations of protease (PRO) and carbohydrase (AMG) were utilized (1:0, 0:1, 2:1, 1:1, 1:2 PRO:AMG) to obtain Water Soluble Enzymatic Hydrolysates (WSEHs). Extraction yields, biochemical and structural characterization, as well as in vitro activity against Herpes simplex virus type 1 (HSV-1) and antioxidant capacities were determined. All PRO:AMG combinations significantly improved yields. EAE yielded heterogeneous extracts rich in iota-carrageenan and phenols, as confirmed by FTIR spectra. The highest antiherpetic activity (EC50 4.5 ± 0.4 μg mL−1) was found in the WSEHs obtained under 2:1 PRO:AMG. At this combination high antioxidant capacity was also obtained for ABTS (2,2′-Azino-Bis-3-ethylbenzoThiazoline-6-Sulfonic acid) radical scavenging activity and Ferric Reducing Antioxidant Power (FRAP). These could probably play a synergistic role associated to the strong antiviral activity obtained. These results suggest that 2:1 PRO:AMG could be effective in promoting the hydrolytic breakdown of high MW polysaccharides, contributing to the improvement of WSEHs bioactivity. Although Solieria filiformis WSEHs showed promising results, further research, including separation and purification techniques are needed.

Published
2021
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37. Ecofriendly silicon-poly(lactic acid) hybrid antifouling coatings

Author

Isabelle Linossier, Karine Réhel, Fabienne Faÿ, Fabrice Azemar, Laboratoire de Biotechnologie et Chimie Marines (LBCM), and Université de Bretagne Sud (UBS)

Subjects
Biocide, Materials science, Fouling, General Chemical Engineering, Organic Chemistry, Artificial seawater, 02 engineering and technology, 010402 general chemistry, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, Surfaces, Coatings and Films, Lactic acid, Biofouling, chemistry.chemical_compound, chemistry, Chemical engineering, Distilled water, Materials Chemistry, Copolymer, [CHIM]Chemical Sciences, Leaching (metallurgy), 0210 nano-technology
Abstract

Societal and environmental constraints have led to changes in international and European regulations to limit the use of biocides in antifouling paints. In this context, coatings combining fouling release properties and leaching of active molecules are sought. The objectives of this study are to conceive a hybrid system for antifouling paint and to observe the influence of the physio-chemical properties of the binders on its antifouling activity. Poly(lactic acid) (PLA) and poly(dimethylsiloxane) (PDMS) homopolymers have already been used as binders for different antifouling strategies. The use of a block copolymer based on PLA and PDMS is likely to allow the design of paint with biocides release associated to fouling release properties in order to be more efficient with a lower environmental impact. Triblock copolymers with different molar compositions have been synthesized. The properties of the corresponding films have been determined in distilled water (adhesion to the support, hydrophobicity). Then formulated paints have been immersed in Lorient harbor to evaluate their erosion, degradation and antifouling efficacy. Furthermore, their biocide release kinetics were studied in artificial seawater to evaluate their environmental impact. The silicon-poly(lactic acid) paints have shown an efficiency superior to a commercial paint during their in situ immersion (19 months) with a lower biocides amount (14 %).

Published
2020
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38. Phidianidine A and Synthetic Analogues as Naturally Inspired Marine Antifoulants

Author

Karine Réhel, Lindon W. K. Moodie, Johan Svenson, Jannie Staffansson, Christophe Labriere, Hugo Denardou, Gunnar Cervin, Jørn H. Hansen, Henrik Pavia, Claire Hellio, Vijayaragavan Elumalai, Tiffany Le Norcy, Department of Chemistry [Tromsø], University of Tromsø (UiT), Department of Marine Sciences [Gothenburg], University of Gothenburg (GU), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Uppsala Universitet [Uppsala], Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), RISE Bioscience and Materials/Chemistry and Materials, and RISE Research Institutes of Sweden

Subjects
Biofouling, Pharmaceutical Science, panorama, 01 natural sciences, Analytical Chemistry, Indole Alkaloids, chemistry.chemical_compound, Marine bacteriophage, Ascomycota, Drug Discovery, Ic50 values, Animals, Seawater, 14. Life underwater, UBO, Pharmacology, Oxadiazoles, Natural product, 010405 organic chemistry, Chemistry, ACL, Organic Chemistry, Thoracica, Combinatorial chemistry, 0104 chemical sciences, 010404 medicinal & biomolecular chemistry, Complementary and alternative medicine, Molecular Medicine, Chemical defense, Pharmacophore, [SDE.BE]Environmental Sciences/Biodiversity and Ecology
Abstract

Stationary and slow-moving marine organisms regularly employ a natural product chemical defense to prevent being colonized by marine micro- and macroorganisms. While these natural antifoulants can be structurally diverse, they often display highly conserved chemistries and physicochemical properties, suggesting a natural marine antifouling pharmacophore. In our current report, we investigate the marine natural product phidianidine A, which displays several chemical properties found in highly potent marine antifoulants. Phidianidine A and synthetic analogues were screened against the settlement and metamorphosis of Amphibalanus improvisus cyprids, and several of the compounds displayed inhibitory activities at low micromolar concentrations with IC50 values down to 0.7 μg/mL observed. The settlement study highlights that phidianidine A is a potent natural antifoulant and that the scaffold can be tuned to generate simpler and improved synthetic analogues. The bioactivity is closely linked to the size of the compound and to its basicity. The study also illustrates that active analogues can be prepared in the absence of the natural constrained 1,2,4-oxadiazole ring. A synthetic lead analogue of phidianidine A was incorporated in a coating and included in antifouling field trials, where it was shown that the coating induced potent inhibition of marine bacteria and microalgae settlement.

Published
2020
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39. Food sources, digestive efficiency and resource allocation in the sea cucumber Holothuria forskali (Echinodermata: Holothuroidea): Insights from pigments and fatty acids

Author

Hélène Laguerre, Gwen Herault, Cédric Hubas, Théo Bordelet, Frank David, Nadia Ameziane, Aicha Badou, Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU), Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biotechnologie et Chimie Marine (LBCM), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Station de Biologie Marine de Concarneau, Direction générale déléguée à la Recherche, à l’Expertise, à la Valorisation et à l’Enseignement-Formation (DGD.REVE), Muséum national d'Histoire naturelle (MNHN)-Muséum national d'Histoire naturelle (MNHN), Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Muséum national d'Histoire naturelle (MNHN), and Muséum national d'Histoire naturelle (MNHN)-École Pratique des Hautes Études (EPHE)

Subjects
0106 biological sciences, 0303 health sciences, Holothuria forskali, biology, 010604 marine biology & hydrobiology, Aquatic Science, biology.organism_classification, 01 natural sciences, 03 medical and health sciences, Sea cucumber, Botany, [SDE]Environmental Sciences, Resource allocation, 14. Life underwater, 030304 developmental biology
Abstract

International audience; Various research projects in Europe and North Africa have recently intended to breed temperate holothurians to alleviate fishing pressure on natural populations. However, to date little is known about the nutritional requirements of East Atlantic and Mediterranean species. In this study, we propose a “natural population”‐oriented approach to characterize food sources, digestive efficiency and resources allocation based on the composition of pigments and fatty acids (FA) in gut contents and tissues (muscles, gonads and digestive tract walls) of wild individuals of the species Holothuria (Panningothuria) forskali (Delle Chiaje, 1823) sampled in Brittany (France). Our study reveals that neither green nor red algae enter the diet of H. forskali in spring and that the only fresh vegetal material found in gut contents is brown algae (very likely diatoms). The high nutritional quality of gut contents however contrasts with the detrital nature of the ingested food sources, suggesting that a trophic upgrading of organic matter occurs before digestion. In addition, unusual FA (i.e. only present in a few groups of living species) such as long‐chain monounsaturated FA (especially the FA 23:1ω9) were found in large proportions in muscles and gonad and their effect on sea cucumber fitness needs further investigation.

Published
2020
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40. Alterocin, an Antibiofilm Protein Secreted by Pseudoalteromonas sp. Strain 3J6

Author

Erwan Corre, David Vallenet, Emilie Portier, Marjolaine Simon, Alexis Bazire, Yannick Fleury, Fanny Gaillard, Angélique Gobet, Gurvan Michel, Morgan Perennou, Albane Jouault, Alain Dufour, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), MARine Biodiversity Exploitation and Conservation (UMR MARBEC), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Fédération de recherche de Roscoff (FR2424), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Analyse Bio-Informatique pour la Génomique et le Métabolisme (LABGeM), Génomique métabolique (UMR 8030), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS)-Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Institut de Recherche pour le Développement (IRD), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE)-Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)-Université d'Évry-Val-d'Essonne (UEVE), Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)

Subjects
Signal peptide, Operon, [SDV]Life Sciences [q-bio], medicine.disease_cause, Applied Microbiology and Biotechnology, biofilm, Microbiology, 03 medical and health sciences, Pseudoalteromonas, Marine bacteriophage, medicine, 14. Life underwater, Gene, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, 0303 health sciences, Ecology, biology, 030306 microbiology, Pseudomonas aeruginosa, Biofilm, biology.organism_classification, 3. Good health, antibiofilm protein, Bacteria, Food Science, Biotechnology
Abstract

We sought to identify and study the antibiofilm protein secreted by the marine bacterium Pseudoalteromonas sp. strain 3J6. The latter is active against marine and terrestrial bacteria, including Pseudomonas aeruginosa clinical strains forming different biofilm types. Several amino acid sequences were obtained from the partially purified antibiofilm protein, named alterocin. The Pseudoalteromonas sp. 3J6 genome was sequenced, and a candidate alt gene was identified by comparing the genome-encoded proteins to the sequences from purified alterocin. Expressing the alt gene in another nonactive Pseudoalteromonas sp. strain, 3J3, demonstrated that it is responsible for the antibiofilm activity. Alterocin is a 139-residue protein that includes a predicted 20-residue signal sequence, which would be cleaved off upon export by the general secretion system. No sequence homology was found between alterocin and proteins of known functions. The alt gene is not part of an operon and adjacent genes do not seem related to alterocin production, immunity, or regulation, suggesting that these functions are not fulfilled by devoted proteins. During growth in liquid medium, the alt mRNA level peaked during the stationary phase. A single promoter was experimentally identified, and several inverted repeats could be binding sites for regulators. alt genes were found in about 30% of the Pseudoalteromonas genomes and in only a few instances of other marine bacteria of the Hahella and Paraglaciecola genera. Comparative genomics yielded the hypothesis that alt gene losses occurred within the Pseudoalteromonas genus. Overall, alterocin is a novel kind of antibiofilm protein of ecological and biotechnological interest. IMPORTANCE Biofilms are microbial communities that develop on solid surfaces or interfaces and are detrimental in a number of fields, including for example food industry, aquaculture, and medicine. In the latter, antibiotics are insufficient to clear biofilm infections, leading to chronic infections such as in the case of infection by Pseudomonas aeruginosa of the lungs of cystic fibrosis patients. Antibiofilm molecules are thus urgently needed to be used in conjunction with conventional antibiotics, as well as in other fields of application, especially if they are environmentally friendly molecules. Here, we describe alterocin, a novel antibiofilm protein secreted by a marine bacterium belonging to the Pseudoalteromonas genus, and its gene. Alterocin homologs were found in about 30% of Pseudoalteromonas strains, indicating that this new family of antibiofilm proteins likely plays an important albeit nonessential function in the biology of these bacteria. This study opens up the possibility of a variety of applications.

Published
2020
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41. Marine Antibiofouling Properties of TiO2 and Ti-Cu-O Films Deposited by Aerosol-Assisted Chemical Vapor Deposition

Author

Carmen Jiménez, Caroline Villardi de Oliveira, Akram Alhussein, Frédéric Sanchette, Frédéric Schuster, Odette Chaix-Pluchery, Jean-Luc Deschanvres, Fabienne Faÿ, Julie Petitbois, School of Biological Sciences, University of Portsmouth, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Systèmes Mécaniques et d'Ingénierie Simultanée (LASMIS), Institut Charles Delaunay (ICD), Université de Technologie de Troyes (UTT)-Centre National de la Recherche Scientifique (CNRS)-Université de Technologie de Troyes (UTT)-Centre National de la Recherche Scientifique (CNRS), Commissariat à l'Energie Atomique, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Laboratoire des matériaux et du génie physique (LMGP ), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), and Université Grenoble Alpes (UGA)-Université Grenoble Alpes (UGA)

Subjects
Anatase, Materials science, Band gap, chemistry.chemical_element, 02 engineering and technology, Chemical vapor deposition, 010402 general chemistry, 01 natural sciences, Catalysis, [SPI.MAT]Engineering Sciences [physics]/Materials, diatoms, Biofouling, Aerosol-CVD, Materials Chemistry, Chemical composition, Fouling, Surfaces and Interfaces, antibiofouling, 021001 nanoscience & nanotechnology, Copper, 0104 chemical sciences, Surfaces, Coatings and Films, chemistry, Chemical engineering, lcsh:TA1-2040, copper, 0210 nano-technology, lcsh:Engineering (General). Civil engineering (General), TiO 2, TiO2
Abstract

The actual interest in developing light-induced catalytic coatings to act as an antibiofouling alternative has recently prompted interest in the incorporation of Cu into TiO2 films, working as a visible light sensitizer catalyst. TiO2 and new Ti-Cu-O films with Cu contents ranging between 16% and 75% Cu/(Cu + Ti) are deposited by aerosol-assisted metalorganic chemical vapor deposition at a substrate temperature of 550 &deg, C. The films are composed of TiO2 anatase phase, mixed with Cu2O when including Cu in the composition. Pure TiO2 films&rsquo, morphologies are characterized by the formation of microflower-like structures with nanometric petals, which induce a high specific surface. These features are not present in Ti-Cu-O films. A UV-Visible study revealed that the optical band gap energy decreases with increasing Cu content. Interestingly, Ti-Cu-O films presented a highly photo-catalytic activity in the orange-G degradation. Marine biofouling field tests in Lorient&rsquo, s Harbor in France and in vitro tests were carried out in order to evaluate the antifouling performance of the films, revealing that topography and chemical composition can act differently on different species. Field tests revealed that TiO2 microflowers reduced the fouling coverage. Besides, Ti-Cu-O films with 16 at.% Cu presented lower fouling coverage than films containing 58 at.% Cu. In vitro tests using two diatoms (P. tricornutum and N. perminuta) showed that the spaces between microflowers play a significant role in the adhesion of diatoms: microalgae adhere less when spaces are bigger than their cells, compared to when spaces are of the same size as cells. Films containing Cu did not alter N. perminuta growth nor adhesion, while they affected P. tricornutum by lowering its growth rate and adhesion without noticeable toxicity. Indeed, Cu-Ti-O is a very promising non-toxic fouling release film for marine and industrial applications.

Published
2020
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42. Défense développée en surface de l’algue rouge Halymenia floresil : profil métabolique et interactions avec les bactéries associées

Author

A Abdul Malik, Shareen, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Bretagne Sud, Nathalie Bourgougnon, and Daniel Robledo

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Quorum sensing, Halymenia floresii, Surface-associated bacteria, Bactéries associées à la surface, Metabolomics, Antifouling, [INFO.INFO-BT]Computer Science [cs]/Biotechnology, Molécules de défense, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Antibiofilm, Defence molecules
Abstract

The surface of Halymenia floresii, a Mexican Rhodophyta, was observed to be remarkably free of epiphytes under Integrated MultiTrophic Aquaculture (IMTA) conditions. This suggests the presence of allelopathic active compounds released by this macroalgae. The aim of this work was to explore the defence mechanisms developed by H. floresii against surface epibiosis, to detect and identify the secondary metabolites produced at the surface of the algae, and to study its relation with surface associated bacteria. For the first time, we isolated the epibacterial community of H. floresii cultivated under controlled conditions (IMTA) and uncontrolled ones (beach-cast material collected in the area). The isolated epibacteria were screened in vitro to analyse Quorum Sensing (QS) signals, and others H. floresii surface extracts were assayed for any QS interference with them. We differentiated the epibacteria significant pathogens from the non- pathogens ones by their ability to induce bleaching, a well-known algal disease. Vibrio owensii was identified as an opportunistic pathogen inducing bleaching in H. floresii which was also associated to the presence of its C4-HSL QS signal. The surface and whole cell metabolites extracts from H. floresii specimens cultivated under controlled conditions were analysed by means of LC/MS. An untargeted metabolomic analysis of H. floresii was performed to provide a global metabolic profile as a first database. We identified ‘41’ active metabolites in H. floresii, among which halogenated compounds, furanones and various inhibitors were overrepresented. Interestingly, the first two classes are well known potent QS interfering compounds. The relatively higher occurrences of allelopathic metabolites at the surface of H. floresii strongly supports the hypothesis that they must be involved in the host protection. Further investigations are needed to explore the secondary metabolites of H. floresii et their role in the seaweed.; Halymenia floresii, une Rhodophycée présente une surface remarquablement exempte d'épiphytes dans les conditions de l'Aquaculture MultiTrophique Intégrée (AMTI). Ce phénomène traduit la présence potentielle en surface de composés actifs allélopathiques. L'objectif de ce travail a été d'explorer les mécanismes de défense développés par H. floresii contre l'épibiose, de détecter et d'identifier les métabolites secondaires produits à la surface de l’algue et d'étudier les relations avec les bactéries épiphytes. Nous avons ainsi pu isoler la communauté épibactérienne de H. floresii cultivée dans des conditions contrôlées (AMTI) et non contrôlées (échantillons collectés in situ). Les épibactéries isolées ont été criblées in vitro pour analyser les signaux de détection de Quorum Sensing (QS). Les extraits produits en surface ont été analysés pour détecter toute interférence avec le Quorum Sensing. Les épibactéries pathogènes et non-pathogènes ont été différenciées par leur capacité à induire une maladie algale, le blanchiment. Vibrio owensii, ainsi que son signal C4-HSL QS, a été identifié comme pathogène opportuniste induisant un blanchiment. Les métabolites extraits de la surface et de cellules entières de H. floresii ont été analysés par LC-MS. Une base de données a été constituée à partir d’une analyse métabolomique non ciblée. Quarante et un métabolites actifs ont été identifiés, parmi lesquels les composés halogénés, des furanones et divers inhibiteurs étaient surreprésentés. Fait intéressant, les deux premières classes sont connues comme de puissants composés interférant avec le QS. La présence relativement élevée de métabolites allélopathiques à la surface de H. floresii soutient fortement l'hypothèse selon laquelle ils doivent être impliqués dans la protection de l'hôte. Des recherches supplémentaires seront nécessaires pour explorer l’ensemble des métabolites secondaires produits par H. floresii et leurs rôles chez l’algue.

Published
2020

43. Influence of Catecholamines (Epinephrine/Norepinephrine) on Biofilm Formation and Adhesion in Pathogenic and Probiotic Strains of Enterococcus faecalis

Author

Linossier, I, Cambronel, Mélyssa, Nilly, Flore, Mesguida, Ouiza, Boukerb, Amine Mohamed, Racine, Pierre-Jean, Baccouri, Olfa, Borrel, Valérie, Martel, Jérome, Fécamp, Florian, Knowlton, Rikki, Zimmermann, Kurt, Domann, Eugen, Rodrigues, Sophie, Feuilloley, Marc, Connil, Nathalie, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), and Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Microbiology (medical), [SDV]Life Sciences [q-bio], lcsh:QR1-502, Context (language use), medicine.disease_cause, Microbiology, lcsh:Microbiology, Enterococcus faecalis, biofilm, law.invention, 03 medical and health sciences, Probiotic, law, medicine, Escherichia coli, ComputingMilieux_MISCELLANEOUS, VicK, 030304 developmental biology, Original Research, 2. Zero hunger, 0303 health sciences, biology, 030306 microbiology, Chemistry, adrenergic sensor, Biofilm, biology.organism_classification, 3. Good health, HaCaT, adhesion, Enterococcus, E. faecalis, Bacteria
Abstract

Enterococcus faecalis has controversial status due to its emerging role in nosocomial infections, while some strains with beneficial effects are used as probiotics and starter cultures in dairy industry. These bacteria can be found as resident or transient germs in the gut or on skin, where they are continually exposed to various eukaryotic molecules. In this context, the aim of our work was to evaluate the effect of the catecholamine stress hormones, epinephrine (Epi), and norepinephrine (NE) on some Enterococcus strains. Four E. faecalis strains were included in this study: E. faecalis MMH594 and E. faecalis V583, pathogenic strains of clinical origin, E. faecalis Symbioflor 1 clone DSM 16431, a pharmaceutical probiotic, and E. faecalis OB15, a probiotic strain previously isolated from Tunisian rigouta (Baccouri et al., 2019). Epi was found to modulate the formation of biofilm (biovolume and thickness) in E. faecalis, whether pathogens or probiotics. NE had less effect on biofilm formation of these bacteria. We also investigated the effect of Epi and NE on adhesion of E. faecalis to eukaryotic cells as it is the first step of colonization of the host. Epi was found to significantly enhance the adhesion of MMH594 and OB15 to Caco-2/TC7 intestinal cells and HaCaT keratinocyte cells, whereas NE significantly increased the adhesion of V583 and Symbioflor 1 DSM 16431 to Caco-2/TC7 cells, the adhesion of MMH594, Symbioflor 1 DSM 16431, and OB15 to HaCaT cells. Analysis of a putative adrenergic sensor of Epi/NE in E. faecalis, compared to QseC, the Escherichia coli adrenergic receptor, allowed the identification of VicK as the nearest protein to QseC with 29% identity and 46% similarity values. Structure modeling and molecular docking of VicK corroborated the hypothesis of possible interactions of this putative adrenergic sensor with Epi and NE, with binding energies of −4.08 and −4.49 kcal/mol, respectively. In conclusion, this study showed for the first time that stress hormones could increase biofilm formation and adhesion to eukaryotic cells in E. faecalis. Future experiments will aim to confirm by in vivo studies the role of VicK as adrenergic sensor in E. faecalis probiotic and pathogen strains. This may help to develop new strategies of antagonism/competition in the gut or skin ecological niches, and to prevent the colonization by opportunistic pathogens.

Published
2020
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44. Surface plasma treatment (Ar/CF4) decreases biofouling on polycarbonate surfaces

Author

Fabienne Poncin-Epaillard, Karine Réhel, Tiffany Le Norcy, Isabelle Linossier, Fabienne Faÿ, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut des Molécules et Matériaux du Mans (IMMM), Le Mans Université (UM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Poncin-Epaillard, Fabienne

Subjects
Materials science, Plasma treatment, surface chemistry, 02 engineering and technology, Anti-microbial, 010402 general chemistry, 01 natural sciences, Biofouling, [CHIM] Chemical Sciences, Materials Chemistry, [CHIM]Chemical Sciences, Polycarbonate, Plasma processing, plasma processing, Process Chemistry and Technology, [SPI.PLASMA]Engineering Sciences [physics]/Plasmas, polymeric materials, Adhesion, Plasma, Antifouling, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Surfaces, Coatings and Films, [CHIM.POLY]Chemical Sciences/Polymers, Chemical engineering, surface energy, visual_art, surface roughness, visual_art.visual_art_medium, 0210 nano-technology
Abstract

International audience; The purpose of this study was to examine the impact of hydrophobicity, surface chemistry, and topography on bacterial and microalgal adhesion. To this end, the effects of surface plasma treatments (Argon and Tetrafluorocarbon) of polycarbonate substrates on bioadhesion were investigated in vitro. Surfaces were characterized by goniometry, atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS). Two marine bacterial strains, a hydrophobic Pseudoalteromonas sp. 5M6 and a hydrophilic Paracoccus sp. 4M6 and two microalgae (Cylindrotheca closterium and Porphyridium purpureum) involved in marine biofouling were selected. Their adhesion on the substrates was observed and quantified using confocal laser scanning microscopy-microfluidic flow-cells. It was demonstrated that the combination of three parameters, namely surface energy, fluorination, and nanotopography, significantly decreases the adhesion of three microorganisms out of four (Pseudoalteromonas sp. 5M6, Cylindrotheca Closterium, and Porphyridium purpureum) whereas one parameter on its own is insufficient.

Published
2020
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45. An Analysis of the Nutritional and Health Values of Caulerpa racemosa (Forsskål) and Ulva fasciata (Delile)—Two Chlorophyta Collected from the Philippines

Author

Magdugo, Rexie, Terme, Nolwenn, Lang, Marie, Pliego-Cortés, Hugo, Marty, Christel, Hurtado, Anicia, Bedoux, Gilles, Bourgougnon, Nathalie, Institut des Sciences Chimiques de Rennes (ISCR), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Ecole Nationale Supérieure de Chimie de Rennes-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biotechnologie et Chimie Marine (LBCM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Université de Bretagne Sud - Vannes (UBS Vannes), and Université de Bretagne Sud (UBS)

Subjects
lipids, amino acids, [SDV]Life Sciences [q-bio], human food, biological activities, green seaweeds, ComputingMilieux_MISCELLANEOUS
Abstract

International audience; Polysaccharides, lipids and amino acid profiles were investigated to understand the nutritional value of Caulerpa racemosa and Ulva fasciata from the Philippines. The results revealed that both species contain high amounts of proteins (8.8-19.9% for C. racemosa and 8.0-11.1% for U. fasciata). The portions of the total amino acids that were essential amino acids (EAAs) (45.28 ± 0.12% for C. racemosa and 42.17 ± 0.12% for U. fasciata) out were comparable to FAO/WHO requirements. Leucine, valine, isoleucine, and lysine are the dominant EAAs in C. racemosa, while leucine, valine, lysine, and phenylalanine are those in U. fasciata. The fatty acid profiles are dominated by monounsaturated fatty acids and polyunsaturated fatty acids in C. racemosa (56.2%), while saturated fatty acids (72.1%) are dominant in U. fasciata. High C18/C20 polyunsaturated fatty acid ratios were recorded in both species. Mineral contents for both seaweeds were within levels considered safe for functional foods. Total pigment content of C. racemosa (140.84 mg/g dw) was almost 20 times higher than that of U. fasciata (7.54 mg/g dw). Hot water extract (HWE) from C. racemosa showed in vitro antiherpetic activity without cytotoxicity. Nutritional characteristics confirmed that C. racemosa could be potentially used as a nutritious and functional food items for human consumption.

Published
2020
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46. Chemical defense against microfouling by allelopathic active metabolites of Halymenia floresii (Rhodophyta)

Author

A Abdul Malik, Shareen, Bedoux, Gilles, Robledo, Daniel, García-Maldonado, José, Freile-Pelegrín, Yolanda, Bourgougnon, Nathalie, Laboratoire de Biotechnologie et Chimie Marine (LBCM), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Bretagne Sud - Vannes (UBS Vannes), and Université de Bretagne Sud (UBS)

Subjects
[SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV]Life Sciences [q-bio], [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2020
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47. Algues marines d'importance économique aux Philippines : évaluation de six espèces Caulerpa racemosa (Forsskål), Ulva fasciata (Delile), Sargassum polycystum (C. Agardh), Sargassum ilicifolium (Turner) C. Agardh, Halymenia durvillei (Bory de Saint-Vincent) et Halymenia dilatata (Zanardini) des Philippines

Author

Magdugo, Rexie, Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Université de Bretagne Sud, Nathalie Bourgougnon, Gilles Bedoux, and STAR, ABES

Subjects
Halymenia, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, [SDV.SA] Life Sciences [q-bio]/Agricultural sciences, Ulva, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, Biological activities, Sargassum, Caulerpa, Activités biologiques, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, [SDV.BIO] Life Sciences [q-bio]/Biotechnology, Nutrition
Abstract

The Philippines is known for its diverse marine flora with more than 1291 taxa of benthic marine algae. They represent a rich natural plant production. Out of 350 species considered to be of economic importance, only 5% are used. Currently at the international level, only the red algae of the genera Eucheuma and Kappaphycus are valued by the production of carrageenans. The objective of this work was to bring new knowledge on 6 seaweeds traditionally used in human food in the villages, confirm their nutritional interests and propose new ways of valorization. The study carried out allowed to highlight and confirmed (1) the nutritional potential of these species by their richness in protein content, the quality of essential amino acids, fatty acids and minerals (2) the presence of parietal polysaccharides with a high yield that can be valorized in the texturizing sector and also presenting antiviral and antioxidant activities interesting the human health sector., Les Philippines sont connues pour sa flore marine diversifiée avec plus de 1291 taxons d'algues benthiques marines. Elles représentent une production végétale naturelle riche. Sur 350 espèces considérées comme d'importance économique, seulement 5% d'entre elles sont utilisées. Actuellement au niveau international, seules les algues rouges des genres Eucheuma et Kappaphycus sont valorisées par la production de carraghénanes. L’objectif de ce travail visait à apporter de nouvelles connaissances sur 6 algues utilisées traditionnellement en alimentation humaine dans les villages, confirmer leurs intérêts nutritionnels et proposer de nouvelles voies de valorisation. L’étude réalisée a permis de mettre en valeur et a confirmé (1) le potentiel nutritionnel de ces espèces par leur richesse en teneur en protéines, la qualité des acides aminés essentiels, des acides gras et des minéraux (2) la présence de polysaccharides pariétaux présentant un rendement élevé pouvant être valorisé dans le secteur des texturants et présentant également des activités antivirales et antioxydantes intéressant le secteur de la santé humaine.

Published
2020

48. Inferring Biochemical Reactions and Metabolite Structures to Understand Metabolic Pathway Drift

Author

Pierre Sauleau, Camille Trottier, Catherine Boyen, Arnaud Belcour, Simon M. Dittami, Gabriel V. Markov, Jean Girard, Charlotte Marteau, Anne Siegel, Jonas Collén, Cédric Leroux, Méziane Aite, Ludovic Delage, Jacques Nicolas, Catherine Leblanc, Erwan Corre, Dynamics, Logics and Inference for biological Systems and Sequences (Dyliss), Inria Rennes – Bretagne Atlantique, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-GESTION DES DONNÉES ET DE LA CONNAISSANCE (IRISA-D7), Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT), Laboratoire de Biologie Intégrative des Modèles Marins (LBI2M), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Station biologique de Roscoff (SBR), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Fédération de recherche de Roscoff (FR2424), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), ABiMS - Informatique et bioinformatique = Analysis and Bioinformatics for Marine Science (ABIMS), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Station biologique de Roscoff (SBR), Scalable, Optimized and Parallel Algorithms for Genomics (GenScale), ANR-10-BTBR-04, Agence Nationale de la Recherche, 9673, Région Bretagne, Université de Bretagne Sud (UBS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National de Recherche en Informatique et en Automatique (Inria)-École normale supérieure - Rennes (ENS Rennes)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-CentraleSupélec-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Bretagne Sud (UBS)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-École normale supérieure - Rennes (ENS Rennes)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), ABiMS - Informatique et bioinformatique = Analysis and Bioinformatics for Marine Science (FR2424), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-Institut National de Recherche en Informatique et en Automatique (Inria)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique), Institut Mines-Télécom [Paris] (IMT)-Institut Mines-Télécom [Paris] (IMT)-Université de Rennes 1 (UR1), and Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Université de Bretagne Sud (UBS)-École normale supérieure - Rennes (ENS Rennes)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-IMT Atlantique Bretagne-Pays de la Loire (IMT Atlantique)

Subjects
0301 basic medicine, Bioinformatics, Metabolite, ved/biology.organism_classification_rank.species, Genomics, 02 engineering and technology, Computational biology, Biology, Article, 03 medical and health sciences, chemistry.chemical_compound, Metabolomics, Chondrus crispus, Genome-scale metabolic network, Model organism, lcsh:Science, Gene, Amino acid synthesis, chemistry.chemical_classification, Metabolic pathway drift, Multidisciplinary, ved/biology, food and beverages, Mycosporine-like amino acid, 021001 nanoscience & nanotechnology, Phenotype, 3. Good health, Metabolic pathway, Sterols, 030104 developmental biology, chemistry, lcsh:Q, [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM], 0210 nano-technology
Abstract

Summary Inferring genome-scale metabolic networks in emerging model organisms is challenged by incomplete biochemical knowledge and partial conservation of biochemical pathways during evolution. Therefore, specific bioinformatic tools are necessary to infer biochemical reactions and metabolic structures that can be checked experimentally. Using an integrative approach combining genomic and metabolomic data in the red algal model Chondrus crispus, we show that, even metabolic pathways considered as conserved, like sterols or mycosporine-like amino acid synthesis pathways, undergo substantial turnover. This phenomenon, here formally defined as “metabolic pathway drift,” is consistent with findings from other areas of evolutionary biology, indicating that a given phenotype can be conserved even if the underlying molecular mechanisms are changing. We present a proof of concept with a methodological approach to formalize the logical reasoning necessary to infer reactions and molecular structures, abstracting molecular transformations based on previous biochemical knowledge., Graphical Abstract, Highlights • Combination of metabolite profiling and genome-scale metabolic networks analysis • New method to infer biochemical reactions and metabolites in promiscuous pathways • Red algal model for sterol and mycosporine-like amino acid biosynthesis pathways • Metabolic drift if pathway variation despite conserved initial and final metabolites, Bioinformatics; Genomics; Metabolomics

Published
2020
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49. Draft Genome Sequences of Four Pseudomonas aeruginosa Clinical Strains with Various Biofilm Phenotypes

Author

Olivier Lesouhaitier, Albane Jouault, Emeline Bouffartigues, Alexis Bazire, Elise Persyn, Emilie Portier, Amine M. Boukerb, Sylvie Chevalier, Jocelyne Caillon, Erwan Pernet, Alain Dufour, Marjolaine Simon, Marc G. J. Feuilloley, Laboratoire de Microbiologie Signaux et Microenvironnement (LMSM), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biotechnologie et Chimie Marine (LBCM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Thérapeutiques cliniques et expérimentales des infections (EA 3826) (EA 3826), Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), and Université de Nantes (UN)-Université de Nantes (UN)

Subjects
congenital, hereditary, and neonatal diseases and abnormalities, 0303 health sciences, Pseudomonas aeruginosa, Genome Sequences, Biofilm, Biology, medicine.disease_cause, medicine.disease, Genome, C content, Phenotype, Cystic fibrosis, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, 3. Good health, Microbiology, 03 medical and health sciences, 0302 clinical medicine, Immunology and Microbiology (miscellaneous), Genetics, medicine, Molecular Biology, Genome size, 030217 neurology & neurosurgery, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology
Abstract

Biofilms produced by Pseudomonas aeruginosa present a serious threat to cystic fibrosis patients. Here, we report the draft genome sequences of four cystic fibrosis isolates displaying various mucoid and biofilm phenotypes. The estimated average genome size was about 6,255,986 ± 50,202 bp with a mean G+C content of 66.52 ± 0.06%.

Published
2020
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50. Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia

Author

Kristiana, Rhesi, Bedoux, Gilles, Pals, Gerard, Mudianta, I. Wayan, Taupin, Laure, Marty, Christel, Asagabaldan, Meezan Ardhanu, Ayuningrum, Diah, Trianto, Agus, Bourgougnon, Nathalie, Radjasa, Ocky Karna, Sabdono, Agus, Hanafi, Muhammad, Laboratoire de Biotechnologie et Chimie Marine (LBCM), Laboratoire de Biotechnologie et Chimie Marines (LBCM), Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Bretagne Sud (UBS)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National de la Recherche Scientifique (CNRS), Clinical Genetics, VU University Medical Center [Amsterdam], Université de Bretagne Sud - Vannes (UBS Vannes), and Université de Bretagne Sud (UBS)

Subjects
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, [SDV]Life Sciences [q-bio], ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2020
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