1. Myoblasts transplanted into rat infarcted myocardium are functionally isolated from their host
- Author
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Serge Charpak, Jean-Thomas Vilquin, Bertrand Léobon, Isabelle Garcin, Etienne Audinat, Philippe Menasché, Laboratoire de Physiologie ESPCI INSERM EPI00-02, Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Physiopathologie et thérapie du muscle strié, Université Pierre et Marie Curie - Paris 6 (UPMC)-IFR14-Institut National de la Santé et de la Recherche Médicale (INSERM), CHU Pitié-Salpêtrière [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Physiopathie cellulaire et moléculaire de l'insuffisance cardiaque, and Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR6
- Subjects
Cardiac function curve ,Cell Transplantation ,Cellular differentiation ,Myoblasts, Skeletal ,[SDV]Life Sciences [q-bio] ,Green Fluorescent Proteins ,Myocardial Infarction ,Biology ,Green fluorescent protein ,Myoblasts ,Fluorescence microscope ,Myocyte ,Animals ,Transplantation, Homologous ,Rats, Wistar ,Muscle, Skeletal ,Cells, Cultured ,Multidisciplinary ,Microscopy, Video ,Myogenesis ,Muscles ,Myocardium ,Cell Differentiation ,Anatomy ,Biological Sciences ,Cell biology ,Rats ,Transplantation ,Electrophysiology ,Luminescent Proteins ,Animals, Newborn ,Microscopy, Fluorescence ,cardiovascular system ,Intracellular - Abstract
Survival and differentiation of myogenic cells grafted into infarcted myocardium have raised the hope that cell transplantation becomes a new therapy for cardiovascular diseases. The approach was further supported by transplantation of skeletal myoblasts, which was shown to improve cardiac performance in several animal species. Despite the success of myoblast transplantation and its recent trial in human, the mechanism responsible for the functional improvement remains unclear. Here, we used intracellular recordings coupled to video and fluorescence microscopy to establish whether myoblasts, genetically labeled with enhanced GFP and transplanted into rat infarcted myocardium, retain excitable and contractile properties, and participate actively to cardiac function. Our results indicate that grafted myoblasts differentiate into peculiar hyperexcitable myotubes with a contractile activity fully independent of neighboring cardiomyocytes. We conclude that mechanisms other than electromechanical coupling between grafted and host cells are involved in the improvement of cardiac function.
- Published
- 2003
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