40 results on '"Lacerda, B"'
Search Results
2. Challenges and Opportunities in Clinical Diagnostic Routine of Envenomation Using Blood Plasma Proteomics
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Joeliton dos Santos Cavalcante, Denis Emanuel Garcia de Almeida, Micael Saggion Moraes, Sophia Ribeiro Santos, Pedro Moriel Pincinato, Pedro Marques Riciopo, Laís Lacerda B. de Oliveira, Wuelton Marcelo Monteiro, and Rui Seabra Ferreira-Junior
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biomarkers ,blood plasma ,clinical proteomics ,human envenoming ,Medicine - Abstract
Specific and sensitive tools for the diagnosis and monitoring of accidents by venomous animals are urgently needed. Several diagnostic and monitoring assays have been developed; however, they have not yet reached the clinic. This has resulted in late diagnoses, which represents one of the main causes of progression from mild to severe disease. Human blood is a protein-rich biological fluid that is routinely collected in hospital settings for diagnostic purposes, which can translate research progress from the laboratory to the clinic. Although it is a limited view, blood plasma proteins provide information about the clinical picture of envenomation. Proteome disturbances in response to envenomation by venomous animals have been identified, allowing mass spectrometry (MS)-based plasma proteomics to emerge as a tool in a range of clinical diagnostics and disease management that can be applied to cases of venomous animal envenomation. Here, we provide a review of the state of the art on routine laboratory diagnoses of envenomation by snakes, scorpions, bees, and spiders, as well as a review of the diagnostic methods and the challenges encountered. We present the state of the art on clinical proteomics as the standardization of procedures to be performed within and between research laboratories, favoring a more excellent peptide coverage of candidate proteins for biomarkers. Therefore, the selection of a sample type and method of preparation should be very specific and based on the discovery of biomarkers in specific approaches. However, the sample collection protocol (e.g., collection tube type) and the processing procedure of the sample (e.g., clotting temperature, time allowed for clotting, and anticoagulant used) are equally important to eliminate any bias.
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- 2023
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3. A soberania estatal e o controle do coronavírus
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FREIRE, R. R., primary, LACERDA, B. V. de, additional, and VIANA, G. P. da H., additional
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- 2021
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4. Estados Unidos e Irã: reflexões sobre a crise diplomática
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FREIRE, R. R., primary, LACERDA, B. V. de, additional, and ROCHA, L. M. P., additional
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- 2021
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5. Decision-making under uncertainty for multi-robot systems
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Lacerda, B, Gautier, A, Rutherford, A, Stephens, A, Street, C, and Hawes, N
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Artificial Intelligence - Abstract
In this overview paper, we present the work of the Goal-Oriented Long-Lived Systems Lab on multi-robot systems. We address multi-robot systems from a decision-making under uncertainty perspective, proposing approaches that explicitly reason about the inherent uncertainty of action execution, and how such stochasticity affects multi-robot coordination. To develop effective decision-making approaches, we take a special focus on (i) temporal uncertainty, in particular of action execution; (ii) the ability to provide rich guarantees of performance, both at a local (robot) level and at a global (team) level; and (iii) scaling up to systems with real-world impact. We summarise several pieces of work and highlight how they address the challenges above, and also hint at future research directions.
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- 2022
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6. Challenges and Opportunities in Clinical Diagnostic Routine of Envenomation Using Blood Plasma Proteomics
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Cavalcante, Joeliton dos Santos, primary, de Almeida, Denis Emanuel Garcia, additional, Moraes, Micael Saggion, additional, Santos, Sophia Ribeiro, additional, Pincinato, Pedro Moriel, additional, Riciopo, Pedro Marques, additional, de Oliveira, Laís Lacerda B., additional, Monteiro, Wuelton Marcelo, additional, and Ferreira-Junior, Rui Seabra, additional
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- 2023
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7. Planning for risk-aversion and expected value in MDPs
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Rigter, M, Duckworth, P, Lacerda, B, and Hawes, N
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FOS: Computer and information sciences ,Artificial Intelligence (cs.AI) ,Computer Science - Artificial Intelligence ,FOS: Electrical engineering, electronic engineering, information engineering ,Systems and Control (eess.SY) ,Electrical Engineering and Systems Science - Systems and Control - Abstract
Planning in Markov decision processes (MDPs) typically optimises the expected cost. However, optimising the expectation does not consider the risk that for any given run of the MDP, the total cost received may be unacceptably high. An alternative approach is to find a policy which optimises a risk-averse objective such as conditional value at risk (CVaR). However, optimising the CVaR alone may result in poor performance in expectation. In this work, we begin by showing that there can be multiple policies which obtain the optimal CVaR. This motivates us to propose a lexicographic approach which minimises the expected cost subject to the constraint that the CVaR of the total cost is optimal. We present an algorithm for this problem and evaluate our approach on four domains. Our results demonstrate that our lexicographic approach improves the expected cost compared to the state of the art algorithm, while achieving the optimal CVaR., Comment: Accepted to ICAPS 2022
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- 2023
8. Risk-constrained planning for multi-agent systems with shared resources
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Gautier, AL, Rigter, M, Lacerda, B, Hawes, N, and Wooldridge, M
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Planning under uncertainty requires complex reasoning about future events, and this complexity increases with the addition of multiple agents. One problem faced when considering multi-agent systems under uncertainty is the handling of shared resources. Adding a resource constraint limits the actions that agents can take, forcing collaborative decision making on who gets to use what resources. Prior work has considered different formulations, such as satisfying a resource constraint in expectation or ensuring that a resource constraint is met some percent of the time. However, these formulations of constrained planning ignore important distributional information about resource usage. Namely, they do not consider how bad the worst cases can get. In this paper, we formulate a risk-constrained shared resource problem and aim to limit the risk of excessive use of such resources. We focus on optimising for reward while constraining the Conditional Value-at-Risk (CVaR) of the shared resource. While CVaR is well studied in the single-agent setting, we consider the challenges that arise from the state and action space explosion in the multi-agent setting. In particular, we exploit risk contributions, a measure introduced in finance research which quantifies how much individual agents affect the joint risk. We present an algorithm that uses risk contributions to iteratively update single-agent policies until the joint risk constraint is satisfied. We evaluate our algorithm on two synthetic domains.
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- 2023
9. Planning with hidden parameter polynomial MDPs
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Costen, C, Rigter, M, Lacerda, B, and Hawes, N
- Abstract
For many applications of Markov Decision Processes (MDPs), the transition function cannot be specified exactly. Bayes-Adaptive MDPs (BAMDPs) extend MDPs to consider transition probabilities governed by latent parameters. To act optimally in BAMDPs, one must maintain a belief distribution over the latent parameters. Typically, this distribution is described by a set of sample (particle) MDPs, and associated weights which represent the likelihood of a sample MDP being the true underlying MDP. However, as the number of dimensions of the latent parameter space increases, the number of sample MDPs required to sufficiently represent the belief distribution grows exponentially. Thus, maintaining an accurate belief in the form of a set of sample MDPs over complex latent spaces is computationally intensive, which in turn affects the performance of planning for these models. In this paper, we propose an alternative approach for maintaining the belief over the latent parameters. We consider a class of BAMDPs where the transition probabilities can be expressed in closed form as a polynomial of the latent parameters, and outline a method to maintain a closed-form belief distribution for the latent parameters which results in an accurate belief representation. Furthermore, the closed-form representation does away with the need to tune the number of sample MDPs required to represent the belief. We evaluate two domains and empirically show that the polynomial, closed-form, belief representation results in better plans than a sampling-based belief representation.
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- 2023
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10. Time-bounded large-scale mission planning under uncertainty for UV disinfection
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Brudermüller, L, Bhattacharyya, R, Lacerda, B, and Hawes, N
- Abstract
The COVID-19 pandemic has motivated research on mobile robot-based disinfection methods to help contain the spread of the virus, including ultraviolet (UV) germicidal inactivation. Recent approaches have focused on formulating autonomous disinfection as a coverage problem. However, the focus so far has been on maximising coverage, rather than scaling solutions to large-scale environments or making solutions robust to environmental uncertainty. Since the intensity of UV light is strongly coupled with the distance to the target surface, localisation errors should be included in the decision making process to synthesise meaningful irradiation durations. Therefore, in this paper we solve a linked path and dosage planning problem, explicitly considering localisation uncertainty in the model. Our model is formulated as a Markov decision process (MDP) which maps localisation uncertainty to dose delivery distributions given radiation and localisation models. We solve this (MDP) over a finite horizon using prioritised value iteration to maximise dose delivery within specified time bounds. Simulation experiments performed on real-world data show successful disinfection, outperforming a rule-based baseline.
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- 2022
11. Probabilistic Planning for AUV Data Harvesting from Smart Underwater Sensor Networks
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Budd, M, Salavasidis, G, Karnarudzaman, I, Harris, CA, Phillips, AB, Duckworth, P, Hawes, N, and Lacerda, B
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Harvesting valuable ocean data, ranging from climate and marine life analysis to industrial equipment monitoring, is an extremely challenging real-world problem. Sparse underwater sensor networks are a promising approach to scale to larger and deeper environments, but these have difficulty offloading their data without external assistance. Traditionally, offloading data has been achieved by costly, fixed communication infrastructure. In this paper, we propose a planning under uncertainty method that enables an autonomous underwater vehicle (AUV) to adaptively collect data from smart sensor networks in underwater environments. Our novel solution exploits the ability of sensor nodes to provide the AUV with time-of-flight acoustic localisation, and is able to prioritise nodes with the most valuable data. In both simulated experiments and a real-world field trial, we demonstrate that our method outperforms the type of hand-designed behaviours that has previously been used in the context of underwater data harvesting.
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- 2022
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12. Bayesian reinforcement learning for single-episode missions in partially unknown environments
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Budd, M, Duckworth, P, Hawes, N, and Lacerda, B
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We consider planning for mobile robots conducting missions in realworld domains where a priori unknown dynamics affect the robot’s costs and transitions. We study single-episode missions where it is crucial that the robot appropriately trades off exploration and exploitation, such that the learning of the environment dynamics is just enough to effectively complete the mission. Thus, we propose modelling unknown dynamics using Gaussian processes, which provide a principled Bayesian framework for incorporating online observations made by the robot, and using them to predict the dynamics in unexplored areas. We then formulate the problem of mission planning in Markov decision processes under Gaussian process predictions as Bayesian model-based reinforcement learning. This allows us to employ solution techniques that plan more efficiently than previous Gaussian process planning methods are able to. We empirically evaluate the benefits of our formulation in an underwater autonomous vehicle navigation task and robot mission planning in a realistic simulation of a nuclear environment.
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- 2022
13. Shared Autonomy Systems with Stochastic Operator Models
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Costen, C, Rigter, M, Lacerda, B, and Hawes, N
- Abstract
We consider shared autonomy systems where multiple operators (AI and human), can interact with the environment, e.g. by controlling a robot. The decision problem for the shared autonomy system is to select which operator takes control at each timestep, such that a reward specifying the intended system behaviour is maximised. The performance of the human operator is influenced by unobserved factors, such as fatigue or skill level. Therefore, the system must reason over stochastic models of operator performance. We present a framework for stochastic operators in shared autonomy systems (SO-SAS), where we represent operators using rich, partially observable models. We formalise SO-SAS as a mixed-observability Markov decision process, where environment states are fully observable and internal operator states are hidden. We test SO-SAS on a simulated domain and a computer game, empirically showing it results in better performance compared to traditional formulations of shared autonomy systems.
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- 2022
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14. Context-aware modelling for multi-robot systems under uncertainty
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Street, C, Lacerda, B, Staniaszek, M, Mühlig, M, and Hawes, N
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- 2022
15. Mixed observability MDPs for shared autonomy with uncertain human behaviour
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Costen, C, Rigter, M, Lacerda, B, and Hawes, N
- Abstract
Shared autonomy allows humans and AI operators to work towards a common goal. Typically, shared autonomy systems are modelled by combining a single model for human behaviour, and a model for the AI behaviour. In this paper, we attempt to provide a richer human model, which accounts for variation in performance due to factors that are not directly observable. Our shared autonomy system will maintain a belief over the unobservable factors, and update its belief as they make observations. The new belief is used to decide who should operate the shared autonomy system. We show that using our model with a richer human representation results in better performance than using a simplistic human model.
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- 2022
16. On Solving a Stochastic Shortest-Path Markov Decision Process as Probabilistic Inference
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Baioumy, M, Lacerda, B, Duckworth, P, and Hawes, N
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FOS: Computer and information sciences ,Computer Science - Machine Learning ,Artificial Intelligence (cs.AI) ,Computer Science - Artificial Intelligence ,MathematicsofComputing_NUMERICALANALYSIS ,ComputingMethodologies_ARTIFICIALINTELLIGENCE ,Machine Learning (cs.LG) - Abstract
Previous work on planning as active inference addresses finite horizon problems and solutions valid for online planning. We propose solving the general Stochastic Shortest-Path Markov Decision Process (SSP MDP) as probabilistic inference. Furthermore, we discuss online and offline methods for planning under uncertainty. In an SSP MDP, the horizon is indefinite and unknown a priori. SSP MDPs generalize finite and infinite horizon MDPs and are widely used in the artificial intelligence community. Additionally, we highlight some of the differences between solving an MDP using dynamic programming approaches widely used in the artificial intelligence community and approaches used in the active inference community., Presented at the second International Workshop on Active Inference (IWAI 2021); 11 pages, 2 figures
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- 2021
17. Time-bounded mission planning in time-varying domains with semi-MDPS and Gaussian processes
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Duckworth, P, Lacerda, B, and Hawes, N
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Uncertain, time-varying dynamic environments are ubiquitous in real world robotics. We propose an online planning framework to address time-bounded missions under time-varying dynamics, where those dynamics affect the duration and outcome of actions. We pose such problems as semi-Markov decision processes, where actions have a duration distributed according to an a priori unknown time-varying function. Our approach maintains a belief over this function, and time is propagated through a discrete search tree that efficiently maintains a subset of reachable states. We show improved mission performance on a marine vehicle simulator acting under real-world spatio-temporal ocean currents, and demonstrate the ability to solve co-safe linear temporal logic problems, which are more complex than the reachability problems tackled in previous approaches.
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- 2021
18. Minimax Regret Optimisation for Robust Planning in Uncertain Markov Decision Processes
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Rigter, M, Lacerda, B, and Hawes, N
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FOS: Computer and information sciences ,Artificial Intelligence (cs.AI) ,Computer Science - Artificial Intelligence ,MathematicsofComputing_NUMERICALANALYSIS ,General Medicine - Abstract
The parameters for a Markov Decision Process (MDP) often cannot be specified exactly. Uncertain MDPs (UMDPs) capture this model ambiguity by defining sets which the parameters belong to. Minimax regret has been proposed as an objective for planning in UMDPs to find robust policies which are not overly conservative. In this work, we focus on planning for Stochastic Shortest Path (SSP) UMDPs with uncertain cost and transition functions. We introduce a Bellman equation to compute the regret for a policy. We propose a dynamic programming algorithm that utilises the regret Bellman equation, and show that it optimises minimax regret exactly for UMDPs with independent uncertainties. For coupled uncertainties, we extend our approach to use options to enable a trade off between computation and solution quality. We evaluate our approach on both synthetic and real-world domains, showing that it significantly outperforms existing baselines., Full version of AAAI 2021 paper, with corrigendum attached that describes error in original paper
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- 2020
19. Multi-robot planning under uncertainty with congestion-aware models
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Street, C, Lacerda, B, Mühlig, M, and Hawes, N
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- 2020
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20. Convex Hull Monte-Carlo Tree Search
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Painter, M, Lacerda, B, and Hawes, N
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FOS: Computer and information sciences ,Artificial Intelligence (cs.AI) ,Computer Science - Artificial Intelligence - Abstract
This work investigates Monte-Carlo planning for agents in stochastic environments, with multiple objectives. We propose the Convex Hull Monte-Carlo Tree-Search (CHMCTS) framework, which builds upon Trial Based Heuristic Tree Search and Convex Hull Value Iteration (CHVI), as a solution to multi-objective planning in large environments. Moreover, we consider how to pose the problem of approximating multiobjective planning solutions as a contextual multi-armed bandits problem, giving a principled motivation for how to select actions from the view of contextual regret. This leads us to the use of Contextual Zooming for action selection, yielding Zooming CHMCTS. We evaluate our algorithm using the Generalised Deep Sea Treasure environment, demonstrating that Zooming CHMCTS can achieve a sublinear contextual regret and scales better than CHVI on a given computational budget., Camera-ready version of paper accepted to ICAPS 2020, along with relevant appendices
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- 2020
21. Policy generation with probabilistic guarantees for long-term autonomy of a mobile robot
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Lacerda, B, Parker, D, and Hawes, N
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- 2018
22. Partial order temporal plan merging for mobile robot tasks
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Mudrova, L, Lacerda, B, and Hawes, N
- Abstract
For many mobile service robot applications, planning problems are based on deciding how and when to navigate to certain locations and execute certain tasks. Typically, many of these tasks are independent from one another, and the main objective is to obtain plans that efficiently take into account where these tasks can be executed and when execution is allowed. In this paper, we present an approach, based on merging of partial order plans with durative actions, that can quickly and effectively generate a plan for a set of independent goals. This plan exploits some of the synergies of the plans for each single task, such as common locations where certain actions should be executed. We evaluate our approach in benchmarking domains, comparing it with state-of-the-art planners and showing how it provides a good trade-off between the approach of sequencing the plans for each task (which is fast but produces poor results), and the approach of planning for a conjunction of all the goals (which is slow but produces good results).
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- 2018
23. Numerical and experimental analysis of the behavior of structural elements composed of double lattice panels filled with cast-in-place concrete
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LACERDA, B. M., primary, LIMA, M. C. V., additional, GESUALDO, F. A. R., additional, and CASTILHO, V. C., additional
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- 2015
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24. Reversible dilated cardiomyopathy associated with amphotericin B therapy
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Soares, J. R., primary, Nunes, M. C. P., additional, Leite, A. F., additional, Falqueto, E. B., additional, Lacerda, B. E. R. A., additional, and Ferrari, T. C. A., additional
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- 2014
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25. LTL-based decentralized supervisory control of multi-robot tasks modelled as Petri nets
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Lacerda, B., primary and Lima, P. U., additional
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- 2011
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26. Occurrence of Agrobacterium tumefaciens Biovar 3 on Grapevine in Brazil
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De Oliveira, J. R., primary, Da Silva Romeiro, R., additional, and De Souza Leäo Lacerda, B., additional
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- 1994
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27. Reversible dilated cardiomyopathy associated with amphotericin B therapy.
- Author
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Soares, J. R., Nunes, M. C. P., Leite, A. F., Falqueto, E. B., Lacerda, B. E. R. A., and Ferrari, T. C. A.
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AMPHOTERICIN B ,DIFFERENTIAL diagnosis ,ECHOCARDIOGRAPHY ,LEISHMANIASIS ,MAGNETIC resonance imaging ,DILATED cardiomyopathy ,THERAPEUTICS - Abstract
What is known and objective: Amphotericin B (AmB) is commonly used to treat a broad spectrum of fungal infections and leishmaniasis. Its use is limited by numerous adverse effects. Reversible dilated cardiomyopathy associated with AmB is a rare disorder with only four previously reported cases, and all of them referring to patients who presented with a predisposing factor for heart failure. Case summary: A previously healthy 45-year-old man with visceral leishmaniasis treated with AmB developed acute dilated cardiomyopathy. Other causes of heart failure as well-known predisposing factors for this condition were ruled out. As with previously reported cases, the cardiac function of our patient returned to normal shortly after. What is new and conclusion: We describe the first case of dilated cardiomyopathy associated with the administration of AmB in a patient without any known predisposing factor for developing cardiac dysfunction. Available evidence suggests that AmB may induce cardiotoxicity. Further investigations are needed to clarify this issue. [ABSTRACT FROM AUTHOR]
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- 2015
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28. Occurrence of <em>Agrobacterium tumefaciens</em> Biovar 3 on Grapevine in Brazil.
- Author
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De Oliveira, J. R., Da Silva Romeiro, R., and De Souza Leáo Lacerda, B.
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GRAPE diseases & pests ,VINEYARDS ,PHYTOPATHOGENIC microorganisms ,AGROBACTERIUM tumefaciens - Abstract
Copyright of Journal of Phytopathology is the property of Wiley-Blackwell and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
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- 1994
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29. Negotiated Path Planning for Non-Cooperative Multi-Robot Systems
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Gautier, A, Stephens, A, Lacerda, B, Hawes, N, and Wooldridge, M
- Abstract
As autonomous systems are deployed at a large scale in both public and private spaces, robots owned and operated by competing organisations will be required to interact. Interactions in such settings will be inherently non-cooperative. In this paper, we address the problem of non-cooperative multi-agent path finding. We design an auction mechanism that allows a group of agents to reach their goals whilst minimising the total cost of the system. In particular, we aim to design a mechanism such that rational agents are incentivised to participate. Our privileged knowledge auction consists of a modified combinatorial Vickrey-Clarke-Groves auction. Our approach limits the initial number of bids in the Vickrey-Clarke-Groves auction, then uses the privileged knowledge of the auctioneer to identify and solve path conflicts. In order to maintain agent autonomy in the non-cooperative system, individual agents are provided with final say over paths. The mechanism provides a heuristic method to maximise social welfare whilst remaining computationally efficient. We also consider single-agent bid generation and propose a similarity metric to use in dissimilar shortest path generation. We then show this bid generation method increases the success likelihood of both the limited-bid VCG auction and our novel approach on synthetic data. Our experiments with synthetic data outperform existing work on the non-cooperative problem.
30. Designing Petri net supervisors for multi-agent systems from LTL specifications
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Lacerda, B. and Pedro Lima
31. Designing Petri net supervisors from LTL specifications
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Lacerda, B. and Pedro Lima
32. Risk-sensitive and robust model-based reinforcement learning and planning
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Rigter, Marc, Hawes, N, and Lacerda, B
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FOS: Computer and information sciences ,Computer Science - Machine Learning ,Artificial intelligence ,Artificial Intelligence (cs.AI) ,Computer Science - Artificial Intelligence ,Machine learning ,Robotics ,Operations research ,Machine Learning (cs.LG) - Abstract
Many sequential decision-making problems that are currently automated, such as those in manufacturing or recommender systems, operate in an environment where there is either little uncertainty, or zero risk of catastrophe. As companies and researchers attempt to deploy autonomous systems in less constrained environments, it is increasingly important that we endow sequential decision-making algorithms with the ability to reason about uncertainty and risk. In this thesis, we will address both planning and reinforcement learning (RL) approaches to sequential decision-making. In the planning setting, it is assumed that a model of the environment is provided, and a policy is optimised within that model. Reinforcement learning relies upon extensive random exploration, and therefore usually requires a simulator in which to perform training. In many real-world domains, it is impossible to construct a perfectly accurate model or simulator. Therefore, the performance of any policy is inevitably uncertain due to the incomplete knowledge about the environment. Furthermore, in stochastic domains, the outcome of any given run is also uncertain due to the inherent randomness of the environment. These two sources of uncertainty are usually classified as epistemic, and aleatoric uncertainty, respectively. The over-arching goal of this thesis is to contribute to developing algorithms that mitigate both sources of uncertainty in sequential decision-making problems. We make a number of contributions towards this goal, with a focus on model-based algorithms..., DPhil (PhD) thesis, University of Oxford
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- 2023
33. Multi-robot coordination under temporal uncertainty
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Street, C, Hawes, N, Lacerda, B, and Mühlig, M
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Planning ,Uncertainty ,Robotics ,Markov Processes - Abstract
Sources of temporal uncertainty affect the duration and start time of robot actions during execution. For example, mobile robots may slip on uneven terrain, slowing them down. The presence of multiple robots in the environment contributes towards temporal uncertainty, as robot interactions such as congestion affect navigation performance. Existing multi-robot coordination solutions often disregard temporal uncertainty through simplifying assumptions such as fixed, identical action durations, which simplifies the problem at the cost of inefficient execution-time behaviour. To synthesise multi-robot behaviour that is robust to unexpected temporal disturbances, we must explicitly capture temporal uncertainty during coordination. In this thesis, we present techniques for effective multi-robot coordination under temporal uncertainty. To represent temporal uncertainty, we construct probabilistic models of action duration for each spatiotemporal situation an action may be executed in. With this, we build formal multi-robot models which accurately capture asynchronous robot execution in continuous time. We then develop planning and task allocation solutions which reason over the effects of temporal uncertainty to synthesise efficient multi-robot behaviour. Further, we consider the problem of policy evaluation, i.e. evaluating properties of synthesised multi-robot behaviour prior to execution, and present two approaches which trade between the accuracy of the model and the solution methods used for policy evaluation. Empirically, our methods outperform solutions which ignore temporal uncertainty, use simplified models, or constrain robot behaviour to reduce temporal uncertainty.
- Published
- 2023
34. O constitucionalismo italiano entre os modelos europeus e a crise do liberalismo
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ALVAZZI DEL FRATE, PAOLO, AA. VV., AMARO LACERDA B. - KOKKE M., and ALVAZZI DEL FRATE, Paolo
- Published
- 2009
35. A missense mutation in the tyrosinase gene explains acromelanism in domesticated canaries.
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Guimarães-Moreira M, Marques CI, Afonso S, Lacerda B, Carneiro M, and Araújo PM
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- Animals, Albinism genetics, Albinism veterinary, Phenotype, Hair Color genetics, Mutation, Missense, Monophenol Monooxygenase genetics, Canaries genetics
- Abstract
Acromelanism is a form of albinism observed in several vertebrate species. In mammals, acromelanism is known to be caused by mutations in the tyrosinase gene (TYR) that induce a temperature-sensitive behavior of melanin synthesis, resulting in a characteristic hair color gradient. In birds, several phenotypes consistent with acromelanism have been reported, but their genetic basis remains unknown. This study aimed to identify the genetic basis of an acromelanistic phenotype in domesticated canaries known as pearl and test whether it is caused by the same molecular mechanism described for mammals. To do this, we compared the genomes of pearl and non-pearl canaries and searched for potentially causative genetic mutations. Our results suggest that the pearl phenotype is caused by a mutation in the TYR gene encoding a TYR-P45H missense substitution. Our findings further suggest that reports of acromelanism in other bird species might be explained by TYR mutations., (© 2024 Stichting International Foundation for Animal Genetics.)
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- 2024
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36. Editorial: Variable autonomy for human-robot teaming.
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Theodorou A, Chiou M, Lacerda B, and Rothfuß S
- Abstract
Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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- 2024
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37. Planning under uncertainty for safe robot exploration using Gaussian process prediction.
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Stephens A, Budd M, Staniaszek M, Casseau B, Duckworth P, Fallon M, Hawes N, and Lacerda B
- Abstract
The exploration of new environments is a crucial challenge for mobile robots. This task becomes even more complex with the added requirement of ensuring safety. Here, safety refers to the robot staying in regions where the values of certain environmental conditions (such as terrain steepness or radiation levels) are within a predefined threshold. We consider two types of safe exploration problems. First, the robot has a map of its workspace, but the values of the environmental features relevant to safety are unknown beforehand and must be explored. Second, both the map and the environmental features are unknown, and the robot must build a map whilst remaining safe. Our proposed framework uses a Gaussian process to predict the value of the environmental features in unvisited regions. We then build a Markov decision process that integrates the Gaussian process predictions with the transition probabilities of the environmental model. The Markov decision process is then incorporated into an exploration algorithm that decides which new region of the environment to explore based on information value, predicted safety, and distance from the current position of the robot. We empirically evaluate the effectiveness of our framework through simulations and its application on a physical robot in an underground environment., Competing Interests: Conflict of interestThe authors declare that they have no conflict of interest., (© The Author(s) 2024, corrected publication 2024.)
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- 2024
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38. Mechanisms of action of fluoridated acidic liquid dentifrices against dental caries.
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Cardoso Cde A, Lacerda B, Mangueira DF, Charone S, Olympio KP, Magalhães AC, Pessan JP, Vilhena FV, Sampaio FC, and Buzalaf MA
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- Brazil, Cariostatic Agents chemistry, Child, Preschool, Dentifrices chemistry, Double-Blind Method, Fluorosis, Dental prevention & control, Hardness, Humans, Hydrogen-Ion Concentration, Infant, Cariostatic Agents pharmacology, Dental Caries prevention & control, Dentifrices pharmacology, Fluorides pharmacology, Phosphates pharmacology
- Abstract
Objective: This study attempted to clarify the mechanisms of action of fluoridated acidic liquid dentifrices against dental caries., Design: In the in vitro leg, enamel specimens were submitted to a pH-cycling model, treated with distinct dentifrices (0, 550 μgF/g pH 4.5 and pH 7.0, 1100 or 5000 μgF/g pH 7.0) and analyzed using hardness. Alkali-soluble fluoride (F) deposition was quantified on pre-demineralized specimens treated with the dentifrices. In the clinical leg, 2-to-4-year-old children who had been using liquid dentifrices for 6 months (550 μgF/g pH 4.5 or pH 7.0 or 1100 μgF/g pH 7.0) had their plaque samples collected 5 and 60 min after the last brushing. Fluoride uptake in whole plaque was evaluated., Results: The reduction of the pH had a partial preventive effect on subsurface hardness loss only. [F] had a significant influence on the deposition of fluoride, surface and subsurface hardness loss. In vivo, the reduction of the pH was able to significantly increase plaque F uptake, leading to similar levels as those found for the neutral dentifrice containing twice [F]., Conclusion: The results obtained from in vitro studies whose design does not include the presence of dental plaque should be interpreted with caution., (Copyright © 2014 Elsevier Ltd. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
39. Proton leak modulation in testicular mitochondria affects reactive oxygen species production and lipid peroxidation.
- Author
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Rodrigues AS, Lacerda B, Moreno AJ, and Ramalho-Santos J
- Subjects
- Animals, Guanosine Diphosphate metabolism, Hydrogen Peroxide metabolism, Ion Channels metabolism, Kidney cytology, Linoleic Acid metabolism, Male, Membrane Potential, Mitochondrial physiology, Mitochondria, Liver metabolism, Mitochondrial Proteins metabolism, Oxidants metabolism, Oxidative Phosphorylation, Oxidative Stress, Oxygen Consumption, Rats, Rats, Wistar, Uncoupling Protein 2, Lipid Peroxidation, Mitochondria metabolism, Protons, Reactive Oxygen Species metabolism, Testis cytology
- Abstract
Mitochondrial proton leak can account for almost 20% of oxygen consumption and it is generally accepted that this process contributes to basal metabolism. In order to clarify the role of basal proton leak in testicular mitochondria, we performed a comparative study with kidney and liver mitochondrial fractions. Proton leak stimulated by linoleic acid and inhibited by guanosine diphosphate (GDP) was detected, in a manner that was correlated with protein levels for uncoupling protein 2 (UCP2) in the three fractions. Modulation of proton leak had an effect on reactive oxygen species production as well as on lipid peroxidation, and this effect was also tissue-dependent. However, a possible role for the adenine nucleotide transporter (ANT) in testicular mitochondria proton leak could not be excluded. The modulation of proton leak appears as a possible and attractive target to control oxidative stress with implications for male gametogenesis., (Copyright (c) 2010 John Wiley & Sons, Ltd.)
- Published
- 2010
- Full Text
- View/download PDF
40. Testicular mitochondrial alterations in untreated streptozotocin-induced diabetic rats.
- Author
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Amaral S, Mota PC, Lacerda B, Alves M, Pereira Mde L, Oliveira PJ, and Ramalho-Santos J
- Subjects
- Animals, Apoptosis, Calcium metabolism, Epididymis pathology, Male, Membrane Potentials, Oxidative Stress, Oxygen Consumption, Rats, Rats, Wistar, Spermatogenesis, Testis pathology, Diabetes Mellitus, Experimental metabolism, Diabetes Mellitus, Experimental pathology, Mitochondria metabolism, Streptozocin pharmacology, Testis metabolism
- Abstract
Diabetes-induced complications are associated with mitochondrial dysfunction and increasing evidence suggests that diabetes has an adverse effect on male reproductive function. The STZ-induced diabetic rat was used as an animal model for the type 1 form of the disease with the aim of determining its effects in spermatogenesis and testicular mitochondrial function. Several aspects of mitochondrial function were measured, including respiratory and electric potential function, as well as mitochondrial calcium loading capacity. Additionally oxidative stress production, antioxidant levels and possible apoptotic alterations were also evaluated. We observed that diabetic animals present alterations in spermatogenesis in both the testis and epidydimus. However, and surprisingly, the overall results in mitochondrial parameters failed to reveal severe testicular mitochondrial dysfunction in diabetic animals, with the exception of a decrease in calcium load. Taken together, results suggest that in animal models that mimic untreated type 1 diabetes the severe effects of the condition on spermatogenesis are not directly mitochondrial-mediated.
- Published
- 2009
- Full Text
- View/download PDF
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